Restriction map for
pF1KSDA0776
(Circular) (Six-base) MAPSORT of: pF1KSDA0776.seq Check: 9444 from: 1 to: 5521
pF1KSDA0776 5521 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1146 1146
Size: 5521
AccI GT'mk_AC
Cuts at: 1916 2557 1916
Size: 641 4880
Acc65I G'GTAC_C
Cuts at: 2535 2535
Size: 5521
AclI AA'CG_TT
Cuts at: 5361 5361
Size: 5521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1337 1867 3344 3776 4525 4793 1135
Size: 202 530 1477 432 749 268 1863
Fragments arranged by size:
1863 1477 749 530 432 268 202
AflIII A'CryG_T
Cuts at: 336 2871 3977 4954 336
Size: 2535 1106 977 903
Fragments arranged by size:
2535 1106 977 903
AgeI A'CCGG_T
Cuts at: 3909 3909
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 964 2118 2824 4393 964
Size: 1154 706 1569 2092
Fragments arranged by size:
2092 1569 1154 706
ApaLI G'TGCA_C
Cuts at: 4291 4291
Size: 5521
ApoI r'AATT_y
Cuts at: 735 928 1225 1955 2282 2523 4874 5130 735
Size: 193 297 730 327 241 2351 256 1126
Fragments arranged by size:
2351 1126 730 327 297 256 241 193
AseI AT'TA_AT
Cuts at: 20 383 20
Size: 363 5158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5521
AvaI C'yCGr_G
Cuts at: 1666 2539 1666
Size: 873 4648
BamHI G'GATC_C
Cuts at: 2544 2544
Size: 5521
BanI G'GyrC_C
Cuts at: 2535 2949 3197 3232 2535
Size: 414 248 35 4824
Fragments arranged by size:
4824 414 248 35
BanII G_rGCy'C
Cuts at: 2533 3563 2533
Size: 1030 4491
BbeI G_GCGC'C
Cuts at: 2953 3201 2953
Size: 248 5273
BbsI GAAGACnn'nnnn_
Cuts at: 1685 2039 2171 2365 1685
Size: 354 132 194 4841
Fragments arranged by size:
4841 354 194 132
BciVI GTATCCnnnnn_n'
Cuts at: 703 3417 4180 5521 703
Size: 2714 763 1341 703
Fragments arranged by size:
2714 1341 763 703
BclI T'GATC_A
Cuts at: 3039 3039
Size: 5521
BglII A'GATC_T
Cuts at: 1323 3034 5073 1323
Size: 1711 2039 1771
Fragments arranged by size:
2039 1771 1711
BlpI GC'TnA_GC
Cuts at: 2631 2631
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 3144 3237 4295 3144
Size: 93 1058 4370
Fragments arranged by size:
4370 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2372 2731 2812 3147 5293 2372
Size: 359 81 335 2146 2600
Fragments arranged by size:
2600 2146 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3886 3886
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 858 284
Size: 574 4947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 2695 3574 4068 4366 808
Size: 1887 879 494 298 1963
Fragments arranged by size:
1963 1887 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5225 5225
Size: 5521
BsaAI yAC'GTr
Cuts at: 691 2511 2872 691
Size: 1820 361 3340
Fragments arranged by size:
3340 1820 361
BsaBI GATnn'nnATC
Cuts at: 6 3033 5072 6
Size: 3027 2039 455
Fragments arranged by size:
3027 2039 455
BsaHI Gr'CG_yC
Cuts at: 134 2950 3198 3902 5398 134
Size: 2816 248 704 1496 257
Fragments arranged by size:
2816 1496 704 257 248
BsaWI w'CCGG_w
Cuts at: 2708 3229 3909 4183 4330 4741 2708
Size: 521 680 274 147 411 3488
Fragments arranged by size:
3488 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 856
Size: 30 5491
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 662 1439 1442 1624 662
Size: 777 3 182 4559
Fragments arranged by size:
4559 777 182 3
BseYI C'CCAG_C
Cuts at: 2944 3702 4281 4777 2944
Size: 758 579 496 3688
Fragments arranged by size:
3688 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 2167
Size: 5521
BsiEI CG_ry'CG
Cuts at: 104 164 3061 3107 4317 104
Size: 60 2897 46 1210 1308
Fragments arranged by size:
2897 1308 1210 60 46
BsiHKAI G_wGCw'C
Cuts at: 2533 3311 3501 4295 2533
Size: 778 190 794 3759
Fragments arranged by size:
3759 794 778 190
BsmI GAATG_Cn'
Cuts at: 1168 1168
Size: 5521
Bsp1286I G_dGCh'C
Cuts at: 2533 3144 3237 3311 3501 3563 4295 2533
Size: 611 93 74 190 62 732 3759
Fragments arranged by size:
3759 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4741 4741
Size: 5521
BspHI T'CATG_A
Cuts at: 4697 4697
Size: 5521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1146 2569 2783 3085 3466 1146
Size: 1423 214 302 381 3201
Fragments arranged by size:
3201 1423 381 302 214
BsrBI CCG'CTC
Cuts at: 50 213 3811 3865 5351 5516 50
Size: 163 3598 54 1486 165 55
Fragments arranged by size:
3598 1486 165 163 55 54
BsrDI GCAATG_nn'
Cuts at: 1446 2261 3431 1446
Size: 815 1170 3536
Fragments arranged by size:
3536 1170 815
BsrFI r'CCGG_y
Cuts at: 3517 3698 3909 3517
Size: 181 211 5129
Fragments arranged by size:
5129 211 181
BsrGI T'GTAC_A
Cuts at: 1240 1766 1240
Size: 526 4995
BssSI C'ACGA_G
Cuts at: 3790 4150 3790
Size: 360 5161
BstAPI GCAn_nnn'nTGC
Cuts at: 2896 2896
Size: 5521
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5521
BstEII G'GTnAC_C
Cuts at: 534 534
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2239
Size: 5521
BstYI r'GATC_y
Cuts at: 1 1323 2544 3011 3034 3369 3615 4629 4715
Size: 1322 1221 467 23 335 246 1014 86
Cuts at: 4715 4727 5073 1
Size: 12 346 449
Fragments arranged by size:
1322 1221 1014 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1607 1607
Size: 5521
BtgI C'CryG_G
Cuts at: 125 696 125
Size: 571 4950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3469 3650 3813 3469
Size: 181 163 5177
Fragments arranged by size:
5177 181 163
BtsI GCAGTG_nn'
Cuts at: 1416 2762 1416
Size: 1346 4175
DraI TTT'AAA
Cuts at: 2518 2518
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 3225 4085 3225
Size: 860 4661
EaeI y'GGCC_r
Cuts at: 161 3104 3278 3669 3696 5043 161
Size: 2943 174 391 27 1347 639
Fragments arranged by size:
2943 1347 639 391 174 27
EagI C'GGCC_G
Cuts at: 161 3104 161
Size: 2943 2578
EarI CTCTTCn'nnn_
Cuts at: 137 494 2498 3542 3752 137
Size: 357 2004 1044 210 1906
Fragments arranged by size:
2004 1906 1044 357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 3727 4039 4185 5331 145
Size: 3582 312 146 1146 335
Fragments arranged by size:
3582 1146 335 312 146
EcoICRI GAG'CTC
Cuts at: 2531 2531
Size: 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 858 1135 1337 1867 3344 3776 4525 4793
Size: 574 277 202 530 1477 432 749 268
Cuts at: 4793 284
Size: 1012
Fragments arranged by size:
1477 1012 749 574 530 432 277 268
202
EcoNI CCTnn'n_nnAGG
Cuts at: 906 906
Size: 5521
EcoO109I rG'GnC_Cy
Cuts at: 2658 2658
Size: 5521
EcoRI G'AATT_C
Cuts at: 735 2523 735
Size: 1788 3733
EcoRV GAT'ATC
Cuts at: 1866 1866
Size: 5521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 3538 2008
Size: 1530 3991
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5521
HaeII r_GCGC'y
Cuts at: 2953 3201 4225 5204 2953
Size: 248 1024 979 3270
Fragments arranged by size:
3270 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 856 888 925 938 957 970 1492 1524 3361
Size: 32 37 13 19 13 522 32 1837
Cuts at: 3361 3391 3393 3423 856
Size: 30 2 30 2954
Fragments arranged by size:
2954 1837 522 37 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 2558 2558
Size: 5521
HindIII A'AGCT_T
Cuts at: 665 2066 665
Size: 1401 4120
KasI G'GCGC_C
Cuts at: 2949 3197 2949
Size: 248 5273
KpnI G_GTAC'C
Cuts at: 2539 2539
Size: 5521
MluI A'CGCG_T
Cuts at: 4954 4954
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2148 4192 4376 2148
Size: 2044 184 3293
Fragments arranged by size:
3293 2044 184
MscI TGG'CCA
Cuts at: 3280 3280
Size: 5521
MslI CAynn'nnrTG
Cuts at: 3508 3635 3508
Size: 127 5394
MspA1I CmG'CkG
Cuts at: 174 730 2630 2944 3304 4319 4564 174
Size: 556 1900 314 360 1015 245 1131
Fragments arranged by size:
1900 1131 1015 556 360 314 245
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 5521
NarI GG'CG_CC
Cuts at: 2950 3198 2950
Size: 248 5273
NcoI C'CATG_G
Cuts at: 125 125
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 5521
NruI TCG'CGA
Cuts at: 3882 3882
Size: 5521
NspI r_CATG'y
Cuts at: 340 445 2229 2302 2572 3072 3981 340
Size: 105 1784 73 270 500 909 1880
Fragments arranged by size:
1880 1784 909 500 270 105 73
PciI A'CATG_T
Cuts at: 336 3977 336
Size: 3641 1880
PfoI T'CCnGG_A
Cuts at: 874 1559 874
Size: 685 4836
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4660 4692 4660
Size: 32 5489
PsiI TTA'TAA
Cuts at: 1231 1231
Size: 5521
PstI C_TGCA'G
Cuts at: 1929 2061 2566 3251 1929
Size: 132 505 685 4199
Fragments arranged by size:
4199 685 505 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5521
PvuII CAG'CTG
Cuts at: 730 2944 3304 730
Size: 2214 360 2947
Fragments arranged by size:
2947 2214 360
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5521
SacI G_AGCT'C
Cuts at: 2533 2533
Size: 5521
SalI G'TCGA_C
Cuts at: 2556 2556
Size: 5521
SapI GCTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5311
SbfI CC_TGCA'GG
Cuts at: 2566 2566
Size: 5521
ScaI AGT'ACT
Cuts at: 888 1636 2424 5038 888
Size: 748 788 2614 1371
Fragments arranged by size:
2614 1371 788 748
SfcI C'TryA_G
Cuts at: 33 725 1190 1925 2057 2562 3247 4242 4433
Size: 692 465 735 132 505 685 995 191
Cuts at: 4433 33
Size: 1121
Fragments arranged by size:
1121 995 735 692 685 505 465 191
132
SfoI GGC'GCC
Cuts at: 2951 3199 2951
Size: 248 5273
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5521
SmaI CCC'GGG
Cuts at: 2541 2541
Size: 5521
SmlI C'TyrA_G
Cuts at: 787 1666 2674 3589 4083 4345 787
Size: 879 1008 915 494 262 1963
Fragments arranged by size:
1963 1008 915 879 494 262
SnaBI TAC'GTA
Cuts at: 2511 2511
Size: 5521
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5521
SphI G_CATG'C
Cuts at: 2229 2302 2572 3072 2229
Size: 73 270 500 4678
Fragments arranged by size:
4678 500 270 73
StuI AGG'CCT
Cuts at: 1183 1183
Size: 5521
StyI C'CwwG_G
Cuts at: 125 2653 3007 125
Size: 2528 354 2639
Fragments arranged by size:
2639 2528 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 3102 3235 3694 3905 882
Size: 2220 133 459 211 2498
Fragments arranged by size:
2498 2220 459 211 133
TatI w'GTAC_w
Cuts at: 886 1240 1357 1451 1634 1766 1858 2422 5036
Size: 354 117 94 183 132 92 564 2614
Cuts at: 5036 886
Size: 1371
Fragments arranged by size:
2614 1371 564 354 183 132 117 94
92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5038 5070 5038
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 3316 3316
Size: 5521
XbaI T'CTAG_A
Cuts at: 924 2550 924
Size: 1626 3895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3014 3014
Size: 5521
XhoI C'TCGA_G
Cuts at: 1666 1666
Size: 5521
XmaI C'CCGG_G
Cuts at: 2539 2539
Size: 5521
XmnI GAAnn'nnTTC
Cuts at: 266 266
Size: 5521
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BssHII BstZ17I ClaI CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI ZraI