Restriction map for
pF1KSDA0778
(Circular) (Six-base) MAPSORT of: pF1KSDA0778.seq Check: 6775 from: 1 to: 6197
pF1KSDA0778 6197 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 165 165
Size: 6197
AatII G_ACGT'C
Cuts at: 2339 2339
Size: 6197
AccI GT'mk_AC
Cuts at: 3235 3235
Size: 6197
Acc65I G'GTAC_C
Cuts at: 612 3213 612
Size: 2601 3596
AclI AA'CG_TT
Cuts at: 6037 6037
Size: 6197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 2146 4022 4454 5201 5469 244
Size: 1902 1876 432 747 268 972
Fragments arranged by size:
1902 1876 972 747 432 268
AflIII A'CryG_T
Cuts at: 1289 3549 4653 5630 1289
Size: 2260 1104 977 1856
Fragments arranged by size:
2260 1856 1104 977
AgeI A'CCGG_T
Cuts at: 1664 4585 1664
Size: 2921 3276
AleI CACnn'nnGTG
Cuts at: 2398 2398
Size: 6197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 977 1009 977
Size: 32 6165
AlwNI CAG_nnn'CTG
Cuts at: 1921 2703 3502 5069 1921
Size: 782 799 1567 3049
Fragments arranged by size:
3049 1567 799 782
ApaI G_GGCC'C
Cuts at: 350 1647 350
Size: 1297 4900
ApaLI G'TGCA_C
Cuts at: 2110 4967 2110
Size: 2857 3340
ApoI r'AATT_y
Cuts at: 1825 3201 5550 5806 1825
Size: 1376 2349 256 2216
Fragments arranged by size:
2349 2216 1376 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6197
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
AvaI C'yCGr_G
Cuts at: 338 805 1814 1901 2470 3217 338
Size: 467 1009 87 569 747 3318
Fragments arranged by size:
3318 1009 747 569 467 87
BamHI G'GATC_C
Cuts at: 3222 3222
Size: 6197
BanI G'GyrC_C
Cuts at: 612 1255 3213 3627 3875 3910 612
Size: 643 1958 414 248 35 2899
Fragments arranged by size:
2899 1958 643 414 248 35
BanII G_rGCy'C
Cuts at: 324 350 583 791 1225 1505 1647 1880 2990
Size: 26 233 208 434 280 142 233 1110
Cuts at: 2990 3211 4241 324
Size: 221 1030 2280
Fragments arranged by size:
2280 1110 1030 434 280 233 233 221
208 142 26
BbeI G_GCGC'C
Cuts at: 3631 3879 3631
Size: 248 5949
BbsI GAAGACnn'nnnn_
Cuts at: 1407 1613 2940 1407
Size: 206 1327 4664
Fragments arranged by size:
4664 1327 206
BbvCI CC'TCA_GC
Cuts at: 617 617
Size: 6197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1263 1297 2266 2300 1263
Size: 34 969 34 5160
Fragments arranged by size:
5160 969 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 4095 4856 6197 3161
Size: 934 761 1341 3161
Fragments arranged by size:
3161 1341 934 761
BclI T'GATC_A
Cuts at: 1001 1955 3717 1001
Size: 954 1762 3481
Fragments arranged by size:
3481 1762 954
BglI GCCn_nnn'nGGC
Cuts at: 169 1122 2251 2590 2760 169
Size: 953 1129 339 170 3606
Fragments arranged by size:
3606 1129 953 339 170
BglII A'GATC_T
Cuts at: 3712 5749 3712
Size: 2037 4160
BlpI GC'TnA_GC
Cuts at: 3309 3309
Size: 6197
Bme1580I G_kGCm'C
Cuts at: 350 1258 1647 2114 2527 2557 3822 3915 4971
Size: 908 389 467 413 30 1265 93 1056
Cuts at: 4971 350
Size: 1576
Fragments arranged by size:
1576 1265 1056 908 467 413 389 93
30
BmgBI CAC'GTC
Cuts at: 1229 1229
Size: 6197
BmrI ACTGGGnnnn_n'
Cuts at: 2713 2776 2793 3409 3490 3825 5969 2713
Size: 63 17 616 81 335 2144 2941
Fragments arranged by size:
2941 2144 616 335 81 63 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1455 4564 1455
Size: 3109 3088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 970 1093 1222 2830 3013 850
Size: 120 123 129 1608 183 4034
Fragments arranged by size:
4034 1608 183 129 123 120
Bpu10I CC'TnA_GC
Cuts at: 323 617 323
Size: 294 5903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2146 3373 4252 4744 5042 1474
Size: 672 1227 879 492 298 2629
Fragments arranged by size:
2629 1227 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 677 701 826 899 1035 1402 5901 677
Size: 24 125 73 136 367 4499 973
Fragments arranged by size:
4499 973 367 136 125 73 24
BsaAI yAC'GTr
Cuts at: 1370 1626 2867 3189 3550 1370
Size: 256 1241 322 361 4017
Fragments arranged by size:
4017 1241 361 322 256
BsaBI GATnn'nnATC
Cuts at: 6 655 3711 5748 6
Size: 649 3056 2037 455
Fragments arranged by size:
3056 2037 649 455
BsaHI Gr'CG_yC
Cuts at: 2336 3628 3876 4578 6074 2336
Size: 1292 248 702 1496 2459
Fragments arranged by size:
2459 1496 1292 702 248
BsaWI w'CCGG_w
Cuts at: 728 908 1664 2792 3386 3907 4585 4859 5006
Size: 180 756 1128 594 521 678 274 147
Cuts at: 5006 5417 728
Size: 411 1508
Fragments arranged by size:
1508 1128 756 678 594 521 411 274
180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 351 381 674 698 704 728 1654 1684 2234
Size: 30 293 24 6 24 926 30 550
Cuts at: 2234 2264 2368 2398 2851 2881 2989 3019 351
Size: 30 104 30 453 30 108 30 3529
Fragments arranged by size:
3529 926 550 453 293 108 104 30
30 30 30 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2419 3010 3094 2419
Size: 591 84 5522
Fragments arranged by size:
5522 591 84
BseYI C'CCAG_C
Cuts at: 270 1218 1749 1773 2201 3161 3622 4380 4957
Size: 948 531 24 428 960 461 758 577
Cuts at: 4957 5453 270
Size: 496 1014
Fragments arranged by size:
1014 960 948 758 577 531 496 461
428 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2923 1705
Size: 1218 4979
BsiEI CG_ry'CG
Cuts at: 104 3739 3785 4993 104
Size: 3635 46 1208 1308
Fragments arranged by size:
3635 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 583 1046 1505 1672 2114 3211 3989 4179 4971
Size: 463 459 167 442 1097 778 190 792
Cuts at: 4971 583
Size: 1809
Fragments arranged by size:
1809 1097 792 778 463 459 442 190
167
BsmI GAATG_Cn'
Cuts at: 1655 2878 1655
Size: 1223 4974
BsmBI CGTCTCn'nnnn_
Cuts at: 1207 2201 2343 2992 1207
Size: 994 142 649 4412
Fragments arranged by size:
4412 994 649 142
Bsp1286I G_dGCh'C
Cuts at: 324 350 583 791 1046 1225 1258 1505 1647
Size: 26 233 208 255 179 33 247 142
Cuts at: 1647 1672 1880 2114 2527 2557 2990 3211 3822
Size: 25 208 234 413 30 433 221 611
Cuts at: 3822 3915 3989 4179 4241 4971 324
Size: 93 74 190 62 730 1550
Fragments arranged by size:
1550 730 611 433 413 255 247 234
233 221 208 208 190 179 142 93
74 62 33 30 26 25
BspEI T'CCGG_A
Cuts at: 728 5417 728
Size: 4689 1508
BspHI T'CATG_A
Cuts at: 2609 5373 2609
Size: 2764 3433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 165 3247 3461 3763 4144 165
Size: 3082 214 302 381 2218
Fragments arranged by size:
3082 2218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 800 1704 3063 4489 4543 6027 6192 50
Size: 750 904 1359 1426 54 1484 165 55
Fragments arranged by size:
1484 1426 1359 904 750 165 55 54
BsrDI GCAATG_nn'
Cuts at: 245 980 1994 2045 2309 2498 4109 245
Size: 735 1014 51 264 189 1611 2333
Fragments arranged by size:
2333 1611 1014 735 264 189 51
BsrFI r'CCGG_y
Cuts at: 1664 4195 4376 4585 1664
Size: 2531 181 209 3276
Fragments arranged by size:
3276 2531 209 181
BssSI C'ACGA_G
Cuts at: 1597 4468 4826 1597
Size: 2871 358 2968
Fragments arranged by size:
2968 2871 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2884 3574 2884
Size: 690 5507
BstBI TT'CG_AA
Cuts at: 110 4558 110
Size: 4448 1749
BstEII G'GTnAC_C
Cuts at: 1962 1962
Size: 6197
BstXI CCAn_nnnn'nTGG
Cuts at: 591 975 1170 1680 2007 2580 2895 591
Size: 384 195 510 327 573 315 3893
Fragments arranged by size:
3893 573 510 384 327 315 195
BstYI r'GATC_y
Cuts at: 1 1697 2156 2972 3222 3689 3712 4047 4293
Size: 1696 459 816 250 467 23 335 246
Cuts at: 4293 5305 5391 5403 5749 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1696 1012 816 467 459 449 346 335
250 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 942 1118 942
Size: 176 6021
BtgI C'CryG_G
Cuts at: 125 132 167 479 1853 2315 125
Size: 7 35 312 1374 462 4007
Fragments arranged by size:
4007 1374 462 312 35 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2427 4147 4328 4491 2427
Size: 1720 181 163 4133
Fragments arranged by size:
4133 1720 181 163
BtsI GCAGTG_nn'
Cuts at: 875 2911 3440 875
Size: 2036 529 3632
Fragments arranged by size:
3632 2036 529
DraI TTT'AAA
Cuts at: 3196 3196
Size: 6197
DraIII CAC_nnn'GTG
Cuts at: 1145 2707 3077 1145
Size: 1562 370 4265
Fragments arranged by size:
4265 1562 370
DrdI GACnn_nn'nnGTC
Cuts at: 1380 3903 4761 1380
Size: 2523 858 2816
Fragments arranged by size:
2816 2523 858
EaeI y'GGCC_r
Cuts at: 141 1106 1130 2252 3782 3956 4347 4374 5719
Size: 965 24 1122 1530 174 391 27 1345
Cuts at: 5719 141
Size: 619
Fragments arranged by size:
1530 1345 1122 965 619 391 174 27
24
EagI C'GGCC_G
Cuts at: 3782 3782
Size: 6197
EarI CTCTTCn'nnn_
Cuts at: 744 4220 4430 744
Size: 3476 210 2511
Fragments arranged by size:
3476 2511 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 975 2764 4405 4715 4861 6007 975
Size: 1789 1641 310 146 1146 1165
Fragments arranged by size:
1789 1641 1165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 581 1503 3209 581
Size: 922 1706 3569
Fragments arranged by size:
3569 1706 922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 850 970 1093 1222 2146 2830 3013 4022
Size: 606 120 123 129 924 684 183 1009
Cuts at: 4022 4454 5201 5469 244
Size: 432 747 268 972
Fragments arranged by size:
1009 972 924 747 684 606 432 268
183 129 123 120
EcoNI CCTnn'n_nnAGG
Cuts at: 298 691 1684 298
Size: 393 993 4811
Fragments arranged by size:
4811 993 393
EcoO109I rG'GnC_Cy
Cuts at: 303 1643 1644 3336 303
Size: 1340 1 1692 3164
Fragments arranged by size:
3164 1692 1340 1
EcoRI G'AATT_C
Cuts at: 3201 3201
Size: 6197
EcoRV GAT'ATC
Cuts at: 2607 2607
Size: 6197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 276 1884 4216 276
Size: 1608 2332 2257
Fragments arranged by size:
2332 2257 1608
FspI TGC'GCA
Cuts at: 3978 3978
Size: 6197
HaeII r_GCGC'y
Cuts at: 3631 3879 4901 5880 3631
Size: 248 1022 979 3948
Fragments arranged by size:
3948 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 698 730 751 783 1345 1377 1654
Size: 32 188 32 21 32 562 32 277
Cuts at: 1654 1686 1881 1913 2122 2154 4039 4069 4071
Size: 32 195 32 209 32 1885 30 2
Cuts at: 4071 4101 478
Size: 30 2574
Fragments arranged by size:
2574 1885 562 277 209 195 188 32
32 32 32 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 1786 2082 3236 1786
Size: 296 1154 4747
Fragments arranged by size:
4747 1154 296
HindIII A'AGCT_T
Cuts at: 1861 1861
Size: 6197
KasI G'GCGC_C
Cuts at: 3627 3875 3627
Size: 248 5949
KpnI G_GTAC'C
Cuts at: 616 3217 616
Size: 2601 3596
MluI A'CGCG_T
Cuts at: 5630 5630
Size: 6197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 764 4868 5052 521
Size: 243 4104 184 1666
Fragments arranged by size:
4104 1666 243 184
MscI TGG'CCA
Cuts at: 1108 1132 3958 1108
Size: 24 2826 3347
Fragments arranged by size:
3347 2826 24
MslI CAynn'nnrTG
Cuts at: 973 1102 1441 1681 1885 1951 2068 2218 2314
Size: 129 339 240 204 66 117 150 96
Cuts at: 2314 2398 2560 2668 2896 4186 4313 973
Size: 84 162 108 228 1290 127 2857
Fragments arranged by size:
2857 1290 339 240 228 204 162 150
129 127 117 108 96 84 66
MspA1I CmG'CkG
Cuts at: 318 532 999 1067 1462 1587 2596 2847 3308
Size: 214 467 68 395 125 1009 251 461
Cuts at: 3308 3622 3982 4995 5240 318
Size: 314 360 1013 245 1275
Fragments arranged by size:
1275 1013 1009 467 461 395 360 314
251 245 214 125 68
NaeI GCC'GGC
Cuts at: 4378 4378
Size: 6197
NarI GG'CG_CC
Cuts at: 3628 3876 3628
Size: 248 5949
NcoI C'CATG_G
Cuts at: 125 479 2315 125
Size: 354 1836 4007
Fragments arranged by size:
4007 1836 354
NgoMIV G'CCGG_C
Cuts at: 4376 4376
Size: 6197
NspI r_CATG'y
Cuts at: 1293 2105 3057 3250 3750 4657 1293
Size: 812 952 193 500 907 2833
Fragments arranged by size:
2833 952 907 812 500 193
PciI A'CATG_T
Cuts at: 1289 4653 1289
Size: 3364 2833
PflMI CCAn_nnn'nTGG
Cuts at: 1629 2703 1629
Size: 1074 5123
PfoI T'CCnGG_A
Cuts at: 635 2990 635
Size: 2355 3842
PmeI GTTT'AAAC
Cuts at: 3196 3196
Size: 6197
PmlI CAC'GTG
Cuts at: 1626 2867 1626
Size: 1241 4956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5336 5368 5336
Size: 32 6165
PspOMI G'GGCC_C
Cuts at: 346 1643 346
Size: 1297 4900
PstI C_TGCA'G
Cuts at: 3244 3929 3244
Size: 685 5512
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6197
PvuII CAG'CTG
Cuts at: 532 999 1067 1462 1587 2596 3622 3982 532
Size: 467 68 395 125 1009 1026 360 2747
Fragments arranged by size:
2747 1026 1009 467 395 360 125 68
RsrII CG'GwC_CG
Cuts at: 4392 4392
Size: 6197
SacI G_AGCT'C
Cuts at: 583 1505 3211 583
Size: 922 1706 3569
Fragments arranged by size:
3569 1706 922
SalI G'TCGA_C
Cuts at: 3234 3234
Size: 6197
SapI GCTCTTCn'nnn_
Cuts at: 4220 4430 4220
Size: 210 5987
SbfI CC_TGCA'GG
Cuts at: 3244 3244
Size: 6197
ScaI AGT'ACT
Cuts at: 151 5714 151
Size: 5563 634
SexAI A'CCwGG_T
Cuts at: 686 3073 686
Size: 2387 3810
SfcI C'TryA_G
Cuts at: 33 1062 3096 3240 3925 4918 5109 33
Size: 1029 2034 144 685 993 191 1121
Fragments arranged by size:
2034 1121 1029 993 685 191 144
SfoI GGC'GCC
Cuts at: 3629 3877 3629
Size: 248 5949
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
SmaI CCC'GGG
Cuts at: 1903 3219 1903
Size: 1316 4881
SmlI C'TyrA_G
Cuts at: 338 1489 2161 3352 4267 4759 5021 338
Size: 1151 672 1191 915 492 262 1514
Fragments arranged by size:
1514 1191 1151 915 672 492 262
SnaBI TAC'GTA
Cuts at: 3189 3189
Size: 6197
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6197
SphI G_CATG'C
Cuts at: 2105 3250 3750 2105
Size: 1145 500 4552
Fragments arranged by size:
4552 1145 500
StuI AGG'CCT
Cuts at: 947 947
Size: 6197
StyI C'CwwG_G
Cuts at: 125 260 299 479 1544 1988 2096 2315 2579
Size: 135 39 180 1065 444 108 219 264
Cuts at: 2579 3331 3685 125
Size: 752 354 2637
Fragments arranged by size:
2637 1065 752 444 354 264 219 180
135 108 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 829 981 1279 2695 3028 3153 3780 3913
Size: 324 152 298 1416 333 125 627 133
Cuts at: 3913 4372 4581 505
Size: 459 209 2121
Fragments arranged by size:
2121 1416 627 459 333 324 298 209
152 133 125
TatI w'GTAC_w
Cuts at: 149 5712 149
Size: 5563 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 351 383 674 706 2234 2266 2851
Size: 32 78 32 291 32 1528 32 585
Cuts at: 2851 2883 5714 5746 241
Size: 32 2831 32 692
Fragments arranged by size:
2831 1528 692 585 291 78 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 712 3994 712
Size: 3282 2915
XbaI T'CTAG_A
Cuts at: 3228 3228
Size: 6197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2103 2704 2805 2895 3692 2103
Size: 601 101 90 797 4608
Fragments arranged by size:
4608 797 601 101 90
XhoI C'TCGA_G
Cuts at: 338 338
Size: 6197
XmaI C'CCGG_G
Cuts at: 1901 3217 1901
Size: 1316 4881
XmnI GAAnn'nnTTC
Cuts at: 2746 2746
Size: 6197
ZraI GAC'GTC
Cuts at: 2337 2337
Size: 6197
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SspI SwaI