Restriction map for
pF1KSDA0780
(Circular) (Six-base) MAPSORT of: pF1KSDA0780.seq Check: 8665 from: 1 to: 6280
pF1KSDA0780 6280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1431 2943 1431
Size: 1512 4768
AccI GT'mk_AC
Cuts at: 1258 1597 3316 1258
Size: 339 1719 4222
Fragments arranged by size:
4222 1719 339
Acc65I G'GTAC_C
Cuts at: 3294 3294
Size: 6280
AclI AA'CG_TT
Cuts at: 6120 6120
Size: 6280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1208 1394 2941 4103 4535 5284 5552 165
Size: 1043 186 1547 1162 432 749 268 893
Fragments arranged by size:
1547 1162 1043 893 749 432 268 186
AflII C'TTAA_G
Cuts at: 1491 2419 1491
Size: 928 5352
AflIII A'CryG_T
Cuts at: 3017 3630 4736 5713 3017
Size: 613 1106 977 3584
Fragments arranged by size:
3584 1106 977 613
AgeI A'CCGG_T
Cuts at: 4668 4668
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 2719 2719
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2304 2304
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 3583 5152 3583
Size: 1569 4711
ApaI G_GGCC'C
Cuts at: 2448 2932 2448
Size: 484 5796
ApaLI G'TGCA_C
Cuts at: 1047 5050 1047
Size: 4003 2277
ApoI r'AATT_y
Cuts at: 928 999 1099 1452 3159 3282 5633 5889 928
Size: 71 100 353 1707 123 2351 256 1319
Fragments arranged by size:
2351 1707 1319 353 256 123 100 71
AseI AT'TA_AT
Cuts at: 20 539 722 3221 20
Size: 519 183 2499 3079
Fragments arranged by size:
3079 2499 519 183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6280
AvaI C'yCGr_G
Cuts at: 1615 3298 1615
Size: 1683 4597
BamHI G'GATC_C
Cuts at: 3043 3303 3043
Size: 260 6020
BanI G'GyrC_C
Cuts at: 516 1838 1896 3294 3708 3956 3991 516
Size: 1322 58 1398 414 248 35 2805
Fragments arranged by size:
2805 1398 1322 414 248 58 35
BanII G_rGCy'C
Cuts at: 249 746 2448 2932 3292 4322 249
Size: 497 1702 484 360 1030 2207
Fragments arranged by size:
2207 1702 1030 497 484 360
BbeI G_GCGC'C
Cuts at: 1842 1900 3712 3960 1842
Size: 58 1812 248 4162
Fragments arranged by size:
4162 1812 248 58
BbsI GAAGACnn'nnnn_
Cuts at: 682 707 1517 682
Size: 25 810 5445
Fragments arranged by size:
5445 810 25
BbvCI CC'TCA_GC
Cuts at: 1390 2939 1390
Size: 1549 4731
BciVI GTATCCnnnnn_n'
Cuts at: 4176 4939 6280 4176
Size: 763 1341 4176
Fragments arranged by size:
4176 1341 763
BclI T'GATC_A
Cuts at: 935 1155 3798 935
Size: 220 2643 3417
Fragments arranged by size:
3417 2643 220
BfrBI ATG'CAT
Cuts at: 838 1543 2555 838
Size: 705 1012 4563
Fragments arranged by size:
4563 1012 705
BglII A'GATC_T
Cuts at: 1867 2333 3793 5832 1867
Size: 466 1460 2039 2315
Fragments arranged by size:
2315 2039 1460 466
BlpI GC'TnA_GC
Cuts at: 2015 3390 2015
Size: 1375 4905
Bme1580I G_kGCm'C
Cuts at: 1051 2358 2448 2649 2932 3903 3996 5054 1051
Size: 1307 90 201 283 971 93 1058 2277
Fragments arranged by size:
2277 1307 1058 971 283 201 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 1763 1793 3490 3571 3906 6052 1763
Size: 30 1697 81 335 2146 1991
Fragments arranged by size:
2146 1991 1697 335 81 30
BmtI G_CTAG'C
Cuts at: 1433 1433
Size: 6280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1683 4645 1683
Size: 2962 3318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 1157 1844 2609 944
Size: 213 687 765 4615
Fragments arranged by size:
4615 765 687 213
Bpu10I CC'TnA_GC
Cuts at: 1390 2939 1390
Size: 1549 4731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1339 3454 4333 4827 5125 785
Size: 554 2115 879 494 298 1940
Fragments arranged by size:
2115 1940 879 554 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2589 5984 2589
Size: 3395 2885
BsaAI yAC'GTr
Cuts at: 3270 3631 3270
Size: 361 5919
BsaBI GATnn'nnATC
Cuts at: 6 3792 5831 6
Size: 3786 2039 455
Fragments arranged by size:
3786 2039 455
BsaHI Gr'CG_yC
Cuts at: 1839 1897 3709 3957 4661 6157 1839
Size: 58 1812 248 704 1496 1962
Fragments arranged by size:
1962 1812 1496 704 248 58
BsaWI w'CCGG_w
Cuts at: 768 3467 3988 4668 4942 5089 5500 768
Size: 2699 521 680 274 147 411 1548
Fragments arranged by size:
2699 1548 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 2936 2966 475
Size: 30 2431 30 3789
Fragments arranged by size:
3789 2431 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 1224 1227 1951 2423 211
Size: 1013 3 724 472 4068
Fragments arranged by size:
4068 1013 724 472 3
BseYI C'CCAG_C
Cuts at: 160 1758 2656 2925 3191 3703 4461 5040 5536
Size: 1598 898 269 266 512 758 579 496
Cuts at: 5536 160
Size: 904
Fragments arranged by size:
1598 904 898 758 579 512 496 269
266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 1007 2052 551
Size: 456 1045 4779
Fragments arranged by size:
4779 1045 456
BsiEI CG_ry'CG
Cuts at: 104 2616 3820 3866 5076 104
Size: 2512 1204 46 1210 1308
Fragments arranged by size:
2512 1308 1210 1204 46
BsiHKAI G_wGCw'C
Cuts at: 1051 2418 3292 4070 4260 5054 1051
Size: 1367 874 778 190 794 2277
Fragments arranged by size:
2277 1367 874 794 778 190
BsiWI C'GTAC_G
Cuts at: 2292 2292
Size: 6280
BsmI GAATG_Cn'
Cuts at: 2392 2392
Size: 6280
Bsp1286I G_dGCh'C
Cuts at: 249 746 1051 2358 2418 2448 2649 2932 3292
Size: 497 305 1307 60 30 201 283 360
Cuts at: 3292 3903 3996 4070 4260 4322 5054 249
Size: 611 93 74 190 62 732 1475
Fragments arranged by size:
1475 1307 732 611 497 360 305 283
201 190 93 74 62 60 30
BspEI T'CCGG_A
Cuts at: 768 5500 768
Size: 4732 1548
BspHI T'CATG_A
Cuts at: 932 2328 5456 932
Size: 1396 3128 1756
Fragments arranged by size:
3128 1756 1396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1431 2943 3157 3328 3542 3844 4225 1431
Size: 1512 214 171 214 302 381 3486
Fragments arranged by size:
3486 1512 381 302 214 214 171
BsrBI CCG'CTC
Cuts at: 50 4570 4624 6110 6275 50
Size: 4520 54 1486 165 55
Fragments arranged by size:
4520 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2112 3062 4190 2112
Size: 950 1128 4202
Fragments arranged by size:
4202 1128 950
BsrFI r'CCGG_y
Cuts at: 4276 4457 4668 4276
Size: 181 211 5888
Fragments arranged by size:
5888 211 181
BssSI C'ACGA_G
Cuts at: 4549 4909 4549
Size: 360 5920
BstAPI GCAn_nnn'nTGC
Cuts at: 2561 3655 2561
Size: 1094 5186
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 132 922 1735 2656 132
Size: 790 813 921 3756
Fragments arranged by size:
3756 921 813 790
BstYI r'GATC_y
Cuts at: 1 1867 2333 3043 3303 3770 3793 4128 4374
Size: 1866 466 710 260 467 23 335 246
Cuts at: 4374 5388 5474 5486 5832 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1866 1014 710 467 466 449 346 335
260 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 281 1274 1919 2894 281
Size: 993 645 975 3667
Fragments arranged by size:
3667 993 975 645
BtgI C'CryG_G
Cuts at: 125 191 1728 2765 125
Size: 66 1537 1037 3640
Fragments arranged by size:
3640 1537 1037 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 418 2819 4228 4409 4572 418
Size: 2401 1409 181 163 2126
Fragments arranged by size:
2401 2126 1409 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 3521 1388
Size: 2133 4147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2944 2979 2944
Size: 35 6245
DraI TTT'AAA
Cuts at: 3277 3277
Size: 6280
DraIII CAC_nnn'GTG
Cuts at: 2059 2059
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 1357 3984 4844 1357
Size: 2627 860 2793
Fragments arranged by size:
2793 2627 860
EaeI y'GGCC_r
Cuts at: 134 431 1645 2039 3863 4037 4428 4455 5802
Size: 297 1214 394 1824 174 391 27 1347
Cuts at: 5802 134
Size: 612
Fragments arranged by size:
1824 1347 1214 612 394 391 297 174
27
EagI C'GGCC_G
Cuts at: 3863 3863
Size: 6280
EarI CTCTTCn'nnn_
Cuts at: 611 1283 1412 1703 2243 3055 3083 4301 4511
Size: 672 129 291 540 812 28 1218 210
Cuts at: 4511 611
Size: 2380
Fragments arranged by size:
2380 1218 812 672 540 291 210 129
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4486 4798 4944 6090 4486
Size: 312 146 1146 4676
Fragments arranged by size:
4676 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3290 3290
Size: 6280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 944 1157 1208 1394 1844 2609 2941 4103
Size: 779 213 51 186 450 765 332 1162
Cuts at: 4103 4535 5284 5552 165
Size: 432 749 268 893
Fragments arranged by size:
1162 893 779 765 749 450 432 332
268 213 186 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1266 2248 1266
Size: 982 5298
EcoO109I rG'GnC_Cy
Cuts at: 1323 3146 3417 1323
Size: 1823 271 4186
Fragments arranged by size:
4186 1823 271
EcoRI G'AATT_C
Cuts at: 928 3159 3282 928
Size: 2231 123 3926
Fragments arranged by size:
3926 2231 123
EcoRV GAT'ATC
Cuts at: 1086 1117 2901 1086
Size: 31 1784 4465
Fragments arranged by size:
4465 1784 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1574 4297 1574
Size: 2723 3557
FspI TGC'GCA
Cuts at: 4059 4059
Size: 6280
HaeII r_GCGC'y
Cuts at: 1842 1900 3712 3960 4984 5963 1842
Size: 58 1812 248 1024 979 2159
Fragments arranged by size:
2159 1812 1024 979 248 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 733 765 1282 1314 1912 1944 2582 2614 2705
Size: 32 517 32 598 32 638 32 91
Cuts at: 2705 2737 2854 2886 2936 2968 3056 3088 4120
Size: 32 117 32 50 32 88 32 1032
Cuts at: 4120 4150 4152 4182 733
Size: 30 2 30 2831
Fragments arranged by size:
2831 1032 638 598 517 117 91 88
50 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1697 2724 3317 1697
Size: 1027 593 4660
Fragments arranged by size:
4660 1027 593
HindIII A'AGCT_T
Cuts at: 1239 1535 1239
Size: 296 5984
HpaI GTT'AAC
Cuts at: 1697 1697
Size: 6280
KasI G'GCGC_C
Cuts at: 1838 1896 3708 3956 1838
Size: 58 1812 248 4162
Fragments arranged by size:
4162 1812 248 58
KpnI G_GTAC'C
Cuts at: 3298 3298
Size: 6280
MluI A'CGCG_T
Cuts at: 5713 5713
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4951 5135 4951
Size: 184 6096
MscI TGG'CCA
Cuts at: 433 1647 2041 4039 433
Size: 1214 394 1998 2674
Fragments arranged by size:
2674 1998 1214 394
MslI CAynn'nnrTG
Cuts at: 130 1733 2764 4267 4394 130
Size: 1603 1031 1503 127 2016
Fragments arranged by size:
2016 1603 1503 1031 127
MspA1I CmG'CkG
Cuts at: 164 429 1487 1762 2014 3389 3703 4063 5078
Size: 265 1058 275 252 1375 314 360 1015
Cuts at: 5078 5323 164
Size: 245 1121
Fragments arranged by size:
1375 1121 1058 1015 360 314 275 265
252 245
NaeI GCC'GGC
Cuts at: 4459 4459
Size: 6280
NarI GG'CG_CC
Cuts at: 1839 1897 3709 3957 1839
Size: 58 1812 248 4162
Fragments arranged by size:
4162 1812 248 58
NcoI C'CATG_G
Cuts at: 125 191 1728 125
Size: 66 1537 4677
Fragments arranged by size:
4677 1537 66
NdeI CA'TA_TG
Cuts at: 948 948
Size: 6280
NgoMIV G'CCGG_C
Cuts at: 4457 4457
Size: 6280
NheI G'CTAG_C
Cuts at: 1429 1429
Size: 6280
NruI TCG'CGA
Cuts at: 4641 4641
Size: 6280
NsiI A_TGCA'T
Cuts at: 840 1545 2557 840
Size: 705 1012 4563
Fragments arranged by size:
4563 1012 705
NspI r_CATG'y
Cuts at: 672 2708 3021 3331 3831 4740 672
Size: 2036 313 310 500 909 2212
Fragments arranged by size:
2212 2036 909 500 313 310
PciI A'CATG_T
Cuts at: 3017 4736 3017
Size: 1719 4561
PflMI CCAn_nnn'nTGG
Cuts at: 752 752
Size: 6280
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3277 3277
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1810 1842 5419 5451 1810
Size: 32 3577 32 2639
Fragments arranged by size:
3577 2639 32 32
PpuMI rG'GwC_Cy
Cuts at: 1323 3146 1323
Size: 1823 4457
PspOMI G'GGCC_C
Cuts at: 2444 2928 2444
Size: 484 5796
PstI C_TGCA'G
Cuts at: 1208 2566 3325 4010 1208
Size: 1358 759 685 3478
Fragments arranged by size:
3478 1358 759 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6280
PvuII CAG'CTG
Cuts at: 164 429 1487 2014 3703 4063 164
Size: 265 1058 527 1689 360 2381
Fragments arranged by size:
2381 1689 1058 527 360 265
RsrII CG'GwC_CG
Cuts at: 4473 4473
Size: 6280
SacI G_AGCT'C
Cuts at: 3292 3292
Size: 6280
SalI G'TCGA_C
Cuts at: 3315 3315
Size: 6280
SapI GCTCTTCn'nnn_
Cuts at: 4301 4511 4301
Size: 210 6070
SbfI CC_TGCA'GG
Cuts at: 3325 3325
Size: 6280
ScaI AGT'ACT
Cuts at: 493 5797 493
Size: 5304 976
SexAI A'CCwGG_T
Cuts at: 3023 3023
Size: 6280
SfcI C'TryA_G
Cuts at: 33 714 1204 2562 3321 4006 5001 5192 33
Size: 681 490 1358 759 685 995 191 1121
Fragments arranged by size:
1358 1121 995 759 685 681 490 191
SfoI GGC'GCC
Cuts at: 1840 1898 3710 3958 1840
Size: 58 1812 248 4162
Fragments arranged by size:
4162 1812 248 58
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6280
SmaI CCC'GGG
Cuts at: 3300 3300
Size: 6280
SmlI C'TyrA_G
Cuts at: 800 1354 1491 2419 3433 4348 4842 5104 800
Size: 554 137 928 1014 915 494 262 1976
Fragments arranged by size:
1976 1014 928 915 554 494 262 137
SnaBI TAC'GTA
Cuts at: 3270 3270
Size: 6280
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6280
SphI G_CATG'C
Cuts at: 3331 3831 3331
Size: 500 5780
SspI AAT'ATT
Cuts at: 448 2224 2995 3009 448
Size: 1776 771 14 3719
Fragments arranged by size:
3719 1776 771 14
StuI AGG'CCT
Cuts at: 1272 1857 1272
Size: 585 5695
StyI C'CwwG_G
Cuts at: 125 191 825 1728 2741 3412 3766 125
Size: 66 634 903 1013 671 354 2639
Fragments arranged by size:
2639 1013 903 671 634 354 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2247 3861 3994 4453 4664 2247
Size: 1614 133 459 211 3863
Fragments arranged by size:
3863 1614 459 211 133
TatI w'GTAC_w
Cuts at: 383 451 491 1495 5795 383
Size: 68 40 1004 4300 868
Fragments arranged by size:
4300 1004 868 68 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5797 5829 5797
Size: 32 6248
Tth111I GACn'n_nGTC
Cuts at: 1339 1995 4075 1339
Size: 656 2080 3544
Fragments arranged by size:
3544 2080 656
XbaI T'CTAG_A
Cuts at: 3309 3309
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3773 3773
Size: 6280
XmaI C'CCGG_G
Cuts at: 3298 3298
Size: 6280
XmnI GAAnn'nnTTC
Cuts at: 418 418
Size: 6280
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AleI AloI AscI AvrII BaeI BcgI
BglI BmgBI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
FseI FspAI MfeI NotI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SwaI XhoI ZraI