Restriction map for
pF1KSDA0782
(Circular) (Six-base) MAPSORT of: pF1KSDA0782.seq Check: 7229 from: 1 to: 6754
pF1KSDA0782 6754 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 467 3466 467
Size: 2999 3755
AccI GT'mk_AC
Cuts at: 988 3422 3740 3790 988
Size: 2434 318 50 3952
Fragments arranged by size:
3952 2434 318 50
Acc65I G'GTAC_C
Cuts at: 2588 3512 3768 2588
Size: 924 256 5574
Fragments arranged by size:
5574 924 256
AclI AA'CG_TT
Cuts at: 6594 6594
Size: 6754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1180 4577 5009 5758 6026 894
Size: 286 3397 432 749 268 1622
Fragments arranged by size:
3397 1622 749 432 286 268
AfeI AGC'GCT
Cuts at: 499 678 1105 2488 499
Size: 179 427 1383 4765
Fragments arranged by size:
4765 1383 427 179
AflIII A'CryG_T
Cuts at: 3609 4104 5210 6187 3609
Size: 495 1106 977 4176
Fragments arranged by size:
4176 1106 977 495
AgeI A'CCGG_T
Cuts at: 5142 5142
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 2268 3720 2268
Size: 1452 5302
AleI CACnn'nnGTG
Cuts at: 1558 3475 1558
Size: 1917 4837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3337 3337
Size: 6754
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2023 2055 2023
Size: 32 6722
AlwNI CAG_nnn'CTG
Cuts at: 630 1037 1521 2157 2400 2950 3317 3628 4057
Size: 407 484 636 243 550 367 311 429
Cuts at: 4057 5626 630
Size: 1569 1758
Fragments arranged by size:
1758 1569 636 550 484 429 407 367
311 243
ApaI G_GGCC'C
Cuts at: 666 1686 2028 2087 2726 3369 666
Size: 1020 342 59 639 643 4051
Fragments arranged by size:
4051 1020 643 639 342 59
ApaLI G'TGCA_C
Cuts at: 2434 2698 5524 2434
Size: 264 2826 3664
Fragments arranged by size:
3664 2826 264
ApoI r'AATT_y
Cuts at: 1531 3756 6107 6363 1531
Size: 2225 2351 256 1922
Fragments arranged by size:
2351 2225 1922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6754
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6754
AvaI C'yCGr_G
Cuts at: 659 1464 1685 1967 3617 3772 659
Size: 805 221 282 1650 155 3641
Fragments arranged by size:
3641 1650 805 282 221 155
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1855 1888 1855
Size: 33 6721
BamHI G'GATC_C
Cuts at: 3777 3777
Size: 6754
BanI G'GyrC_C
Cuts at: 1301 1560 2588 3512 3768 4182 4430 4465 1301
Size: 259 1028 924 256 414 248 35 3590
Fragments arranged by size:
3590 1028 924 414 259 256 248 35
BanII G_rGCy'C
Cuts at: 146 355 666 1056 1202 1686 2028 2063 2087
Size: 209 311 390 146 484 342 35 24
Cuts at: 2087 2516 2726 2828 2996 3369 3542 3605 3766
Size: 429 210 102 168 373 173 63 161
Cuts at: 3766 4796 146
Size: 1030 2104
Fragments arranged by size:
2104 1030 484 429 390 373 342 311
210 209 173 168 161 146 102 63
35 24
BbeI G_GCGC'C
Cuts at: 4186 4434 4186
Size: 248 6506
BbsI GAAGACnn'nnnn_
Cuts at: 2064 2395 2783 2064
Size: 331 388 6035
Fragments arranged by size:
6035 388 331
BbvCI CC'TCA_GC
Cuts at: 977 1058 2987 977
Size: 81 1929 4744
Fragments arranged by size:
4744 1929 81
BciVI GTATCCnnnnn_n'
Cuts at: 441 4650 5413 6754 441
Size: 4209 763 1341 441
Fragments arranged by size:
4209 1341 763 441
BclI T'GATC_A
Cuts at: 453 560 4272 453
Size: 107 3712 2935
Fragments arranged by size:
3712 2935 107
BglI GCCn_nnn'nGGC
Cuts at: 589 1631 2511 3178 3260 589
Size: 1042 880 667 82 4083
Fragments arranged by size:
4083 1042 880 667 82
BglII A'GATC_T
Cuts at: 4267 6306 4267
Size: 2039 4715
BlpI GC'TnA_GC
Cuts at: 190 647 3864 190
Size: 457 3217 3080
Fragments arranged by size:
3217 3080 457
Bme1580I G_kGCm'C
Cuts at: 660 666 1256 1686 1811 2028 2087 2438 2702
Size: 6 590 430 125 217 59 351 264
Cuts at: 2702 2726 3139 3369 4377 4470 5528 660
Size: 24 413 230 1008 93 1058 1886
Fragments arranged by size:
1886 1058 1008 590 430 413 351 264
230 217 125 93 59 24 6
BmrI ACTGGGnnnn_n'
Cuts at: 1077 1253 3964 4045 4380 6526 1077
Size: 176 2711 81 335 2146 1305
Fragments arranged by size:
2711 2146 1305 335 176 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2598 5119 2598
Size: 2521 4233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1330 1906 2425 2566 2568 3022 751
Size: 579 576 519 141 2 454 4483
Fragments arranged by size:
4483 579 576 519 454 141 2
Bpu10I CC'TnA_GC
Cuts at: 292 977 1058 1732 1928 2011 2827 2987 292
Size: 685 81 674 196 83 816 160 4059
Fragments arranged by size:
4059 816 685 674 196 160 83 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 2467 3928 4807 5301 5599 2425
Size: 42 1461 879 494 298 3580
Fragments arranged by size:
3580 1461 879 494 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 1414 2586 3367 6458 1414
Size: 1172 781 3091 1710
Fragments arranged by size:
3091 1710 1172 781
BsaAI yAC'GTr
Cuts at: 528 762 2460 3744 4105 528
Size: 234 1698 1284 361 3177
Fragments arranged by size:
3177 1698 1284 361 234
BsaBI GATnn'nnATC
Cuts at: 6 452 4266 6305 6
Size: 446 3814 2039 455
Fragments arranged by size:
3814 2039 455 446
BsaHI Gr'CG_yC
Cuts at: 260 1139 1989 4183 4431 5135 6631 260
Size: 879 850 2194 248 704 1496 383
Fragments arranged by size:
2194 1496 879 850 704 383 248
BsaWI w'CCGG_w
Cuts at: 1547 3341 3941 4462 5142 5416 5563 5974 1547
Size: 1794 600 521 680 274 147 411 2327
Fragments arranged by size:
2327 1794 680 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 934 964 1057 1087 1303 1333 2023 2053 934
Size: 30 93 30 216 30 690 30 5635
Fragments arranged by size:
5635 690 216 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 820 1046 1384 2584 2587 2857 3004 3091
Size: 666 226 338 1200 3 270 147 87
Cuts at: 3091 154
Size: 3817
Fragments arranged by size:
3817 1200 666 338 270 226 147 87
3
BseYI C'CCAG_C
Cuts at: 307 687 1212 1225 1276 1288 1447 1983 2226
Size: 380 525 13 51 12 159 536 243
Cuts at: 2226 2528 2894 2970 3132 3461 3639 4177 4935
Size: 302 366 76 162 329 178 538 758
Cuts at: 4935 5514 6010 307
Size: 579 496 1051
Fragments arranged by size:
1051 758 579 538 536 525 496 380
366 329 302 243 178 162 159 76
51 13 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1130 2901 3079 3109 3592 805
Size: 325 1771 178 30 483 3967
Fragments arranged by size:
3967 1771 483 325 178 30
BsiEI CG_ry'CG
Cuts at: 104 1619 3294 4294 4340 5550 104
Size: 1515 1675 1000 46 1210 1308
Fragments arranged by size:
1675 1515 1308 1210 1000 46
BsiHKAI G_wGCw'C
Cuts at: 1121 1202 1838 2063 2438 2702 2828 2996 3542
Size: 81 636 225 375 264 126 168 546
Cuts at: 3542 3766 4544 4734 5528 1121
Size: 224 778 190 794 2347
Fragments arranged by size:
2347 794 778 636 546 375 264 225
224 190 168 126 81
BsmI GAATG_Cn'
Cuts at: 1933 1933
Size: 6754
BsmBI CGTCTCn'nnnn_
Cuts at: 1146 1146
Size: 6754
Bsp1286I G_dGCh'C
Cuts at: 146 355 660 666 1056 1121 1202 1256 1686
Size: 209 305 6 390 65 81 54 430
Cuts at: 1686 1811 1838 2028 2063 2087 2438 2516 2702
Size: 125 27 190 35 24 351 78 186
Cuts at: 2702 2726 2828 2996 3139 3369 3542 3605 3766
Size: 24 102 168 143 230 173 63 161
Cuts at: 3766 4377 4470 4544 4734 4796 5528 146
Size: 611 93 74 190 62 732 1372
Fragments arranged by size:
1372 732 611 430 390 351 305 230
209 190 190 186 173 168 161 143
125 102 93 81 78 74 65 63
62 54 35 27 24 24 6
BspEI T'CCGG_A
Cuts at: 3341 5974 3341
Size: 2633 4121
BspHI T'CATG_A
Cuts at: 1905 5930 1905
Size: 4025 2729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 2510 3061 3466 3802 4016 4318 4699 467
Size: 2043 551 405 336 214 302 381 2522
Fragments arranged by size:
2522 2043 551 405 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 609 1353 1752 1980 2202 3362 5044 5098
Size: 559 744 399 228 222 1160 1682 54
Cuts at: 5098 6584 6749 50
Size: 1486 165 55
Fragments arranged by size:
1682 1486 1160 744 559 399 228 222
165 55 54
BsrDI GCAATG_nn'
Cuts at: 4664 4664
Size: 6754
BsrFI r'CCGG_y
Cuts at: 872 1657 1700 2902 3383 4750 4931 5142 872
Size: 785 43 1202 481 1367 181 211 2484
Fragments arranged by size:
2484 1367 1202 785 481 211 181 43
BsrGI T'GTAC_A
Cuts at: 1850 2186 1850
Size: 336 6418
BssHII G'CGCG_C
Cuts at: 2425 2425
Size: 6754
BssSI C'ACGA_G
Cuts at: 5023 5383 5023
Size: 360 6394
BstAPI GCAn_nnn'nTGC
Cuts at: 630 1521 4129 630
Size: 891 2608 3255
Fragments arranged by size:
3255 2608 891
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 1413 1491 2743 1413
Size: 78 1252 5424
Fragments arranged by size:
5424 1252 78
BstYI r'GATC_y
Cuts at: 1 412 1956 3005 3777 4244 4267 4602 4848
Size: 411 1544 1049 772 467 23 335 246
Cuts at: 4848 5862 5948 5960 6306 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1544 1049 1014 772 467 449 411 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1475 3276 3629 1475
Size: 1801 353 4600
Fragments arranged by size:
4600 1801 353
BtgI C'CryG_G
Cuts at: 163 332 641 767 1122 1824 3173 3355 3710
Size: 169 309 126 355 702 1349 182 355
Cuts at: 3710 163
Size: 3207
Fragments arranged by size:
3207 1349 702 355 355 309 182 169
126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 957 4702 4883 5046 957
Size: 3745 181 163 2665
Fragments arranged by size:
3745 2665 181 163
BtsI GCAGTG_nn'
Cuts at: 952 1763 1811 2344 2849 2879 3514 3629 3995
Size: 811 48 533 505 30 635 115 366
Cuts at: 3995 952
Size: 3711
Fragments arranged by size:
3711 811 635 533 505 366 115 48
30
DraI TTT'AAA
Cuts at: 3751 3751
Size: 6754
DraIII CAC_nnn'GTG
Cuts at: 2339 2796 3153 2339
Size: 457 357 5940
Fragments arranged by size:
5940 457 357
DrdI GACnn_nn'nnGTC
Cuts at: 4458 5318 4458
Size: 860 5894
EaeI y'GGCC_r
Cuts at: 179 764 1001 2763 3197 4337 4511 4902 4929
Size: 585 237 1762 434 1140 174 391 27
Cuts at: 4929 6276 179
Size: 1347 657
Fragments arranged by size:
1762 1347 1140 657 585 434 391 237
174 27
EagI C'GGCC_G
Cuts at: 4337 4337
Size: 6754
EarI CTCTTCn'nnn_
Cuts at: 230 1207 1685 2087 2521 2737 2816 2927 3731
Size: 977 478 402 434 216 79 111 804
Cuts at: 3731 4775 4985 230
Size: 1044 210 1999
Fragments arranged by size:
1999 1044 977 804 478 434 402 216
210 111 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 287 4960 5272 5418 6564 287
Size: 4673 312 146 1146 477
Fragments arranged by size:
4673 1146 477 312 146
EcoICRI GAG'CTC
Cuts at: 1200 2061 2826 2994 3540 3764 1200
Size: 861 765 168 546 224 4190
Fragments arranged by size:
4190 861 765 546 224 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 894 1180 1330 1906 2425 2566 2568 3022
Size: 143 286 150 576 519 141 2 454
Cuts at: 3022 4577 5009 5758 6026 751
Size: 1555 432 749 268 1479
Fragments arranged by size:
1555 1479 749 576 519 454 432 286
268 150 143 141 2
EcoNI CCTnn'n_nnAGG
Cuts at: 634 1951 2416 2443 634
Size: 1317 465 27 4945
Fragments arranged by size:
4945 1317 465 27
EcoO109I rG'GnC_Cy
Cuts at: 627 981 1266 1383 1470 1479 1728 2083 2084
Size: 354 285 117 87 9 249 355 1
Cuts at: 2084 2640 2891 2967 3365 3366 3891 627
Size: 556 251 76 398 1 525 3490
Fragments arranged by size:
3490 556 525 398 355 354 285 251
249 117 87 76 9 1 1
EcoRI G'AATT_C
Cuts at: 1531 3756 1531
Size: 2225 4529
EcoRV GAT'ATC
Cuts at: 2298 2298
Size: 6754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4771 4771
Size: 6754
FspI TGC'GCA
Cuts at: 3733 4533 3733
Size: 800 5954
HaeII r_GCGC'y
Cuts at: 501 680 1107 1136 1226 1432 1707 2482 2490
Size: 179 427 29 90 206 275 775 8
Cuts at: 2490 2535 3090 4186 4434 5458 6437 501
Size: 45 555 1096 248 1024 979 818
Fragments arranged by size:
1096 1024 979 818 775 555 427 275
248 206 179 90 45 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 404 436 475 507 970 1002 1057
Size: 32 179 32 39 32 463 32 55
Cuts at: 1057 1089 2254 2286 2413 2445 2458 2490 2997
Size: 32 1165 32 127 32 13 32 507
Cuts at: 2997 3029 3706 3738 4594 4624 4626 4656 193
Size: 32 677 32 856 30 2 30 2291
Fragments arranged by size:
2291 1165 856 677 507 463 179 127
55 39 32 32 32 32 32 32
32 32 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 2622 3066 3791 2622
Size: 444 725 5585
Fragments arranged by size:
5585 725 444
KasI G'GCGC_C
Cuts at: 4182 4430 4182
Size: 248 6506
KpnI G_GTAC'C
Cuts at: 2592 3516 3772 2592
Size: 924 256 5574
Fragments arranged by size:
5574 924 256
MfeI C'AATT_G
Cuts at: 2711 2711
Size: 6754
MluI A'CGCG_T
Cuts at: 3609 6187 3609
Size: 2578 4176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 932 2252 5425 5609 932
Size: 1320 3173 184 2077
Fragments arranged by size:
3173 2077 1320 184
MscI TGG'CCA
Cuts at: 181 3199 4513 181
Size: 3018 1314 2422
Fragments arranged by size:
3018 2422 1314
MslI CAynn'nnrTG
Cuts at: 251 841 847 1558 2462 2903 3475 3532 4741
Size: 590 6 711 904 441 572 57 1209
Cuts at: 4741 4868 251
Size: 127 2137
Fragments arranged by size:
2137 1209 904 711 590 572 441 127
57 6
MspA1I CmG'CkG
Cuts at: 1212 2022 2100 2318 2397 2838 3317 3712 3863
Size: 810 78 218 79 441 479 395 151
Cuts at: 3863 4177 4537 5552 5797 1212
Size: 314 360 1015 245 2169
Fragments arranged by size:
2169 1015 810 479 441 395 360 314
245 218 151 79 78
NaeI GCC'GGC
Cuts at: 1659 1702 4933 1659
Size: 43 3231 3480
Fragments arranged by size:
3480 3231 43
NarI GG'CG_CC
Cuts at: 4183 4431 4183
Size: 248 6506
NcoI C'CATG_G
Cuts at: 332 641 1824 3173 332
Size: 309 1183 1349 3913
Fragments arranged by size:
3913 1349 1183 309
NgoMIV G'CCGG_C
Cuts at: 1657 1700 4931 1657
Size: 43 3231 3480
Fragments arranged by size:
3480 3231 43
NruI TCG'CGA
Cuts at: 5115 5115
Size: 6754
NspI r_CATG'y
Cuts at: 2902 3805 4305 5214 2902
Size: 903 500 909 4442
Fragments arranged by size:
4442 909 903 500
PciI A'CATG_T
Cuts at: 5210 5210
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 2157 2796 3153 3174 3461 2157
Size: 639 357 21 287 5450
Fragments arranged by size:
5450 639 357 287 21
PfoI T'CCnGG_A
Cuts at: 2075 3008 3542 2075
Size: 933 534 5287
Fragments arranged by size:
5287 933 534
PmeI GTTT'AAAC
Cuts at: 3751 3751
Size: 6754
PmlI CAC'GTG
Cuts at: 528 2460 528
Size: 1932 4822
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2055 5893 5925 2023
Size: 32 3838 32 2852
Fragments arranged by size:
3838 2852 32 32
PpuMI rG'GwC_Cy
Cuts at: 1470 1728 2891 2967 1470
Size: 258 1163 76 5257
Fragments arranged by size:
5257 1163 258 76
PspOMI G'GGCC_C
Cuts at: 662 1682 2024 2083 2722 3365 662
Size: 1020 342 59 639 643 4051
Fragments arranged by size:
4051 1020 643 639 342 59
PstI C_TGCA'G
Cuts at: 635 1023 1274 1398 1526 2120 3799 4484 635
Size: 388 251 124 128 594 1679 685 2905
Fragments arranged by size:
2905 1679 685 594 388 251 128 124
PvuI CG_AT'CG
Cuts at: 104 3294 104
Size: 3190 3564
PvuII CAG'CTG
Cuts at: 1212 3317 4177 4537 1212
Size: 2105 860 360 3429
Fragments arranged by size:
3429 2105 860 360
RsrII CG'GwC_CG
Cuts at: 4947 4947
Size: 6754
SacI G_AGCT'C
Cuts at: 1202 2063 2828 2996 3542 3766 1202
Size: 861 765 168 546 224 4190
Fragments arranged by size:
4190 861 765 546 224 168
SacII CC_GC'GG
Cuts at: 3713 3713
Size: 6754
SalI G'TCGA_C
Cuts at: 3789 3789
Size: 6754
SanDI GG'GwC_CC
Cuts at: 1470 2891 1470
Size: 1421 5333
SapI GCTCTTCn'nnn_
Cuts at: 1207 2087 2521 2737 2816 4775 4985 1207
Size: 880 434 216 79 1959 210 2976
Fragments arranged by size:
2976 1959 880 434 216 210 79
SbfI CC_TGCA'GG
Cuts at: 3799 3799
Size: 6754
ScaI AGT'ACT
Cuts at: 6271 6271
Size: 6754
SexAI A'CCwGG_T
Cuts at: 2150 3149 2150
Size: 999 5755
SfcI C'TryA_G
Cuts at: 33 631 1019 1270 1394 1522 1669 2017 2116
Size: 598 388 251 124 128 147 348 99
Cuts at: 2116 3795 4480 5475 5666 33
Size: 1679 685 995 191 1121
Fragments arranged by size:
1679 1121 995 685 598 388 348 251
191 147 128 124 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 3178 3178
Size: 6754
SfoI GGC'GCC
Cuts at: 4184 4432 4184
Size: 248 6506
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6754
SmaI CCC'GGG
Cuts at: 661 1687 1969 3619 3774 661
Size: 1026 282 1650 155 3641
Fragments arranged by size:
3641 1650 1026 282 155
SmlI C'TyrA_G
Cuts at: 2440 2482 3907 4822 5316 5578 2440
Size: 42 1425 915 494 262 3616
Fragments arranged by size:
3616 1425 915 494 262 42
SnaBI TAC'GTA
Cuts at: 3744 3744
Size: 6754
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6754
SphI G_CATG'C
Cuts at: 2902 3805 4305 2902
Size: 903 500 5351
Fragments arranged by size:
5351 903 500
SrfI GCCC'GGGC
Cuts at: 661 661
Size: 6754
StuI AGG'CCT
Cuts at: 2551 2551
Size: 6754
StyI C'CwwG_G
Cuts at: 222 332 641 1145 1716 1824 3173 3886 4240
Size: 110 309 504 571 108 1349 713 354
Cuts at: 4240 222
Size: 2736
Fragments arranged by size:
2736 1349 713 571 504 354 309 110
108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 426 1837 3468 4335 4468 4927 5138 426
Size: 1411 1631 867 133 459 211 2042
Fragments arranged by size:
2042 1631 1411 867 459 211 133
TatI w'GTAC_w
Cuts at: 1850 2186 2657 6269 1850
Size: 336 471 3612 2335
Fragments arranged by size:
3612 2335 471 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6271 6303 6271
Size: 32 6722
Tth111I GACn'n_nGTC
Cuts at: 1726 4549 1726
Size: 2823 3931
XbaI T'CTAG_A
Cuts at: 801 3783 801
Size: 2982 3772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 820 1354 1410 3458 4247 820
Size: 534 56 2048 789 3327
Fragments arranged by size:
3327 2048 789 534 56
XmaI C'CCGG_G
Cuts at: 659 1685 1967 3617 3772 659
Size: 1026 282 1650 155 3641
Fragments arranged by size:
3641 1650 1026 282 155
XmnI GAAnn'nnTTC
Cuts at: 1234 1535 1234
Size: 301 6453
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BcgI BfrBI BmgBI BmtI
BsiWI BstEII BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI NdeI NheI NotI NsiI PacI PasI PshAI
PsiI PspXI PsrI SgrAI SspI SwaI XhoI ZraI