Restriction map for
pF1KSDA0789
(Circular) (Six-base) MAPSORT of: pF1KSDA0789.seq Check: 6880 from: 1 to: 4834
pF1KSDA0789 4834 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 770 1870 770
Size: 1100 3734
Acc65I G'GTAC_C
Cuts at: 1848 1848
Size: 4834
AclI AA'CG_TT
Cuts at: 4674 4674
Size: 4834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2657 3089 3838 4106 1594
Size: 1063 432 749 268 2322
Fragments arranged by size:
2322 1063 749 432 268
AfeI AGC'GCT
Cuts at: 1684 1684
Size: 4834
AflIII A'CryG_T
Cuts at: 890 1187 2184 3290 4267 890
Size: 297 997 1106 977 1457
Fragments arranged by size:
1457 1106 997 977 297
AgeI A'CCGG_T
Cuts at: 3222 3222
Size: 4834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1040 1172 1564 1040
Size: 132 392 4310
Fragments arranged by size:
4310 392 132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1111 1143 1111
Size: 32 4802
AlwNI CAG_nnn'CTG
Cuts at: 1180 2137 3706 1180
Size: 957 1569 2308
Fragments arranged by size:
2308 1569 957
ApaI G_GGCC'C
Cuts at: 552 552
Size: 4834
ApaLI G'TGCA_C
Cuts at: 1558 3604 1558
Size: 2046 2788
ApoI r'AATT_y
Cuts at: 145 1836 4187 4443 145
Size: 1691 2351 256 536
Fragments arranged by size:
2351 1691 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4834
AvaI C'yCGr_G
Cuts at: 261 1702 1852 261
Size: 1441 150 3243
Fragments arranged by size:
3243 1441 150
BamHI G'GATC_C
Cuts at: 1857 1857
Size: 4834
BanI G'GyrC_C
Cuts at: 652 751 955 1848 2262 2510 2545 652
Size: 99 204 893 414 248 35 2941
Fragments arranged by size:
2941 893 414 248 204 99 35
BanII G_rGCy'C
Cuts at: 468 552 1197 1694 1846 2876 468
Size: 84 645 497 152 1030 2426
Fragments arranged by size:
2426 1030 645 497 152 84
BbeI G_GCGC'C
Cuts at: 656 755 2266 2514 656
Size: 99 1511 248 2976
Fragments arranged by size:
2976 1511 248 99
BbsI GAAGACnn'nnnn_
Cuts at: 216 1741 216
Size: 1525 3309
BbvCI CC'TCA_GC
Cuts at: 302 302
Size: 4834
BciVI GTATCCnnnnn_n'
Cuts at: 2730 3493 4834 2730
Size: 763 1341 2730
Fragments arranged by size:
2730 1341 763
BclI T'GATC_A
Cuts at: 2352 2352
Size: 4834
BfrBI ATG'CAT
Cuts at: 336 336
Size: 4834
BglI GCCn_nnn'nGGC
Cuts at: 1402 1436 1619 1402
Size: 34 183 4617
Fragments arranged by size:
4617 183 34
BglII A'GATC_T
Cuts at: 2347 4386 2347
Size: 2039 2795
BlpI GC'TnA_GC
Cuts at: 311 1944 311
Size: 1633 3201
Bme1580I G_kGCm'C
Cuts at: 552 1029 1562 2457 2550 3608 552
Size: 477 533 895 93 1058 1778
Fragments arranged by size:
1778 1058 895 533 477 93
BmrI ACTGGGnnnn_n'
Cuts at: 2044 2125 2460 4606 2044
Size: 81 335 2146 2272
Fragments arranged by size:
2272 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 4834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 664 477
Size: 187 4647
Bpu10I CC'TnA_GC
Cuts at: 302 1686 302
Size: 1384 3450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 454 1516 2008 2887 3381 3679 357
Size: 97 1062 492 879 494 298 1512
Fragments arranged by size:
1512 1062 879 494 492 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 4538 4538
Size: 4834
BsaAI yAC'GTr
Cuts at: 1190 1824 2185 1190
Size: 634 361 3839
Fragments arranged by size:
3839 634 361
BsaBI GATnn'nnATC
Cuts at: 6 589 2346 4385 6
Size: 583 1757 2039 455
Fragments arranged by size:
2039 1757 583 455
BsaHI Gr'CG_yC
Cuts at: 653 752 1361 2263 2511 3215 4711 653
Size: 99 609 902 248 704 1496 776
Fragments arranged by size:
1496 902 776 704 609 248 99
BsaWI w'CCGG_w
Cuts at: 1694 2021 2542 3222 3496 3643 4054 1694
Size: 327 521 680 274 147 411 2474
Fragments arranged by size:
2474 680 521 411 327 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 572 1060 572
Size: 488 4346
BseYI C'CCAG_C
Cuts at: 211 297 1596 1617 2257 3015 3594 4090 211
Size: 86 1299 21 640 758 579 496 955
Fragments arranged by size:
1299 955 758 640 579 496 86 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 903 1056 836
Size: 67 153 4614
Fragments arranged by size:
4614 153 67
BsiEI CG_ry'CG
Cuts at: 104 2374 2420 3630 104
Size: 2270 46 1210 1308
Fragments arranged by size:
2270 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1562 1846 2624 2814 3608 1562
Size: 284 778 190 794 2788
Fragments arranged by size:
2788 794 778 284 190
Bsp1286I G_dGCh'C
Cuts at: 468 552 1029 1197 1562 1694 1846 2457 2550
Size: 84 477 168 365 132 152 611 93
Cuts at: 2550 2624 2814 2876 3608 468
Size: 74 190 62 732 1694
Fragments arranged by size:
1694 732 611 477 365 190 168 152
132 93 84 74 62
BspEI T'CCGG_A
Cuts at: 1694 4054 1694
Size: 2360 2474
BspHI T'CATG_A
Cuts at: 4010 4010
Size: 4834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 794 1882 2096 2398 2779 377
Size: 417 1088 214 302 381 2432
Fragments arranged by size:
2432 1088 417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 996 1284 3124 3178 4664 4829 50
Size: 946 288 1840 54 1486 165 55
Fragments arranged by size:
1840 1486 946 288 165 55 54
BsrDI GCAATG_nn'
Cuts at: 377 1154 2744 377
Size: 777 1590 2467
Fragments arranged by size:
2467 1590 777
BsrFI r'CCGG_y
Cuts at: 773 1640 2830 3011 3222 773
Size: 867 1190 181 211 2385
Fragments arranged by size:
2385 1190 867 211 181
BssSI C'ACGA_G
Cuts at: 3103 3463 3103
Size: 360 4474
BstAPI GCAn_nnn'nTGC
Cuts at: 743 949 962 2209 743
Size: 206 13 1247 3368
Fragments arranged by size:
3368 1247 206 13
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4834
BstEII G'GTnAC_C
Cuts at: 625 625
Size: 4834
BstXI CCAn_nnnn'nTGG
Cuts at: 1669 1669
Size: 4834
BstYI r'GATC_y
Cuts at: 1 677 1857 2324 2347 2682 2928 3942 4028
Size: 676 1180 467 23 335 246 1014 86
Cuts at: 4028 4040 4386 1
Size: 12 346 449
Fragments arranged by size:
1180 1014 676 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1501 125
Size: 1376 3458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2782 2963 3126 2782
Size: 181 163 4490
Fragments arranged by size:
4490 181 163
BtsI GCAGTG_nn'
Cuts at: 824 2075 824
Size: 1251 3583
DraI TTT'AAA
Cuts at: 1275 1831 1275
Size: 556 4278
DrdI GACnn_nn'nnGTC
Cuts at: 1588 2538 3398 1588
Size: 950 860 3024
Fragments arranged by size:
3024 950 860
EaeI y'GGCC_r
Cuts at: 128 669 1163 1214 1335 1428 1474 2417 2591
Size: 541 494 51 121 93 46 943 174
Cuts at: 2591 2982 3009 4356 128
Size: 391 27 1347 606
Fragments arranged by size:
1347 943 606 541 494 391 174 121
93 51 46 27
EagI C'GGCC_G
Cuts at: 2417 2417
Size: 4834
EarI CTCTTCn'nnn_
Cuts at: 494 2855 3065 494
Size: 2361 210 2263
Fragments arranged by size:
2361 2263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 1797 3040 3352 3498 4644 151
Size: 1646 1243 312 146 1146 341
Fragments arranged by size:
1646 1243 1146 341 312 146
EcoICRI GAG'CTC
Cuts at: 1844 1844
Size: 4834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 664 1594 2657 3089 3838 4106 477
Size: 187 930 1063 432 749 268 1205
Fragments arranged by size:
1205 1063 930 749 432 268 187
EcoNI CCTnn'n_nnAGG
Cuts at: 1576 1576
Size: 4834
EcoO109I rG'GnC_Cy
Cuts at: 549 1569 1581 1971 549
Size: 1020 12 390 3412
Fragments arranged by size:
3412 1020 390 12
EcoRI G'AATT_C
Cuts at: 1836 1836
Size: 4834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 4834
FspI TGC'GCA
Cuts at: 1448 2613 1448
Size: 1165 3669
HaeII r_GCGC'y
Cuts at: 656 755 1686 2266 2514 3538 4517 656
Size: 99 931 580 248 1024 979 973
Fragments arranged by size:
1024 979 973 931 580 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 980 1012 1295 1327 2674 2704 2706 2736 980
Size: 32 283 32 1347 30 2 30 3078
Fragments arranged by size:
3078 1347 283 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1871 1871
Size: 4834
HindIII A'AGCT_T
Cuts at: 433 433
Size: 4834
KasI G'GCGC_C
Cuts at: 652 751 2262 2510 652
Size: 99 1511 248 2976
Fragments arranged by size:
2976 1511 248 99
KpnI G_GTAC'C
Cuts at: 1852 1852
Size: 4834
MluI A'CGCG_T
Cuts at: 4267 4267
Size: 4834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3505 3689 3505
Size: 184 4650
MscI TGG'CCA
Cuts at: 130 1165 1216 1476 2593 130
Size: 1035 51 260 1117 2371
Fragments arranged by size:
2371 1117 1035 260 51
MslI CAynn'nnrTG
Cuts at: 895 1756 2821 2948 895
Size: 861 1065 127 2781
Fragments arranged by size:
2781 1065 861 127
MspA1I CmG'CkG
Cuts at: 153 256 283 301 783 877 939 1301 1596
Size: 103 27 18 482 94 62 362 295
Cuts at: 1596 1943 2257 2617 3632 3877 153
Size: 347 314 360 1015 245 1110
Fragments arranged by size:
1110 1015 482 362 360 347 314 295
245 103 94 62 27 18
NaeI GCC'GGC
Cuts at: 3013 3013
Size: 4834
NarI GG'CG_CC
Cuts at: 653 752 2263 2511 653
Size: 99 1511 248 2976
Fragments arranged by size:
2976 1511 248 99
NcoI C'CATG_G
Cuts at: 125 125
Size: 4834
NgoMIV G'CCGG_C
Cuts at: 3011 3011
Size: 4834
NruI TCG'CGA
Cuts at: 3195 3195
Size: 4834
NsiI A_TGCA'T
Cuts at: 338 338
Size: 4834
NspI r_CATG'y
Cuts at: 336 894 1453 1731 1885 2385 3294 336
Size: 558 559 278 154 500 909 1876
Fragments arranged by size:
1876 909 559 558 500 278 154
PciI A'CATG_T
Cuts at: 890 3290 890
Size: 2400 2434
PflMI CCAn_nnn'nTGG
Cuts at: 137 1180 137
Size: 1043 3791
PfoI T'CCnGG_A
Cuts at: 353 485 353
Size: 132 4702
PmeI GTTT'AAAC
Cuts at: 1831 1831
Size: 4834
PmlI CAC'GTG
Cuts at: 1190 1190
Size: 4834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3973 4005 3973
Size: 32 4802
PpuMI rG'GwC_Cy
Cuts at: 1569 1581 1569
Size: 12 4822
PshAI GACnn'nnGTC
Cuts at: 1096 1096
Size: 4834
PspOMI G'GGCC_C
Cuts at: 548 548
Size: 4834
PspXI vC'TCGA_Gb
Cuts at: 1702 1702
Size: 4834
PstI C_TGCA'G
Cuts at: 291 782 1879 2564 291
Size: 491 1097 685 2561
Fragments arranged by size:
2561 1097 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4834
PvuII CAG'CTG
Cuts at: 301 783 877 1596 2257 2617 301
Size: 482 94 719 661 360 2518
Fragments arranged by size:
2518 719 661 482 360 94
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 4834
SacI G_AGCT'C
Cuts at: 1846 1846
Size: 4834
SalI G'TCGA_C
Cuts at: 1869 1869
Size: 4834
SapI GCTCTTCn'nnn_
Cuts at: 494 2855 3065 494
Size: 2361 210 2263
Fragments arranged by size:
2361 2263 210
SbfI CC_TGCA'GG
Cuts at: 1879 1879
Size: 4834
ScaI AGT'ACT
Cuts at: 4351 4351
Size: 4834
SfcI C'TryA_G
Cuts at: 33 287 778 1875 2560 3555 3746 33
Size: 254 491 1097 685 995 191 1121
Fragments arranged by size:
1121 1097 995 685 491 254 191
SfoI GGC'GCC
Cuts at: 654 753 2264 2512 654
Size: 99 1511 248 2976
Fragments arranged by size:
2976 1511 248 99
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4834
SmaI CCC'GGG
Cuts at: 1854 1854
Size: 4834
SmlI C'TyrA_G
Cuts at: 372 469 1531 1702 1987 2902 3396 3658 372
Size: 97 1062 171 285 915 494 262 1548
Fragments arranged by size:
1548 1062 915 494 285 262 171 97
SnaBI TAC'GTA
Cuts at: 1824 1824
Size: 4834
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4834
SphI G_CATG'C
Cuts at: 1453 1885 2385 1453
Size: 432 500 3902
Fragments arranged by size:
3902 500 432
StuI AGG'CCT
Cuts at: 1354 1354
Size: 4834
StyI C'CwwG_G
Cuts at: 125 1966 2320 125
Size: 1841 354 2639
Fragments arranged by size:
2639 1841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 553 2415 2548 3007 3218 316
Size: 237 1862 133 459 211 1932
Fragments arranged by size:
1932 1862 459 237 211 133
TatI w'GTAC_w
Cuts at: 512 4349 512
Size: 3837 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 803 835 4351 4383 803
Size: 32 3516 32 1254
Fragments arranged by size:
3516 1254 32 32
Tth111I GACn'n_nGTC
Cuts at: 2629 2629
Size: 4834
XbaI T'CTAG_A
Cuts at: 1863 1863
Size: 4834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1518 2327 1518
Size: 809 4025
XhoI C'TCGA_G
Cuts at: 1702 1702
Size: 4834
XmaI C'CCGG_G
Cuts at: 1852 1852
Size: 4834
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BcgI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI PacI PasI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI XmnI ZraI