(Circular) (Six-base) MAPSORT of: pF1KSDA0797.seq Check: 6910 from: 1 to: 6475 pF1KSDA0797 6475 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AccI GT'mk_AC Cuts at: 528 865 1473 2918 3511 528 Size: 337 608 1445 593 3492 Fragments arranged by size: 3492 1445 608 593 337 Acc65I G'GTAC_C Cuts at: 3489 3489 Size: 6475 AclI AA'CG_TT Cuts at: 6315 6315 Size: 6475 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2242 2528 2906 3189 3373 4298 4730 5479 5747 Size: 286 378 283 184 925 432 749 268 Cuts at: 5747 2242 Size: 2970 Fragments arranged by size: 2970 925 749 432 378 286 283 268 184 AflII C'TTAA_G Cuts at: 2847 2847 Size: 6475 AflIII A'CryG_T Cuts at: 452 3825 4931 5908 452 Size: 3373 1106 977 1019 Fragments arranged by size: 3373 1106 1019 977 AgeI A'CCGG_T Cuts at: 4863 4863 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1702 1702 Size: 6475 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 503 535 503 Size: 32 6443 AlwNI CAG_nnn'CTG Cuts at: 3212 3778 5347 3212 Size: 566 1569 4340 Fragments arranged by size: 4340 1569 566 ApaLI G'TGCA_C Cuts at: 5245 5245 Size: 6475 ApoI r'AATT_y Cuts at: 373 900 1746 1871 2014 2164 2243 2743 3092 Size: 527 846 125 143 150 79 500 349 Cuts at: 3092 3285 3477 5828 6084 373 Size: 193 192 2351 256 764 Fragments arranged by size: 2351 846 764 527 500 349 256 193 192 150 143 125 79 AseI AT'TA_AT Cuts at: 20 641 1826 20 Size: 621 1185 4669 Fragments arranged by size: 4669 1185 621 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6475 AvaI C'yCGr_G Cuts at: 3493 3493 Size: 6475 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 744 777 744 Size: 33 6442 BamHI G'GATC_C Cuts at: 3498 3498 Size: 6475 BanI G'GyrC_C Cuts at: 3489 3903 4151 4186 3489 Size: 414 248 35 5778 Fragments arranged by size: 5778 414 248 35 BanII G_rGCy'C Cuts at: 3175 3487 4517 3175 Size: 312 1030 5133 Fragments arranged by size: 5133 1030 312 BbeI G_GCGC'C Cuts at: 3907 4155 3907 Size: 248 6227 BbsI GAAGACnn'nnnn_ Cuts at: 391 1301 2237 391 Size: 910 936 4629 Fragments arranged by size: 4629 936 910 BbvCI CC'TCA_GC Cuts at: 1184 1184 Size: 6475 BciVI GTATCCnnnnn_n' Cuts at: 3052 4371 5134 6475 3052 Size: 1319 763 1341 3052 Fragments arranged by size: 3052 1341 1319 763 BclI T'GATC_A Cuts at: 234 3993 234 Size: 3759 2716 BglII A'GATC_T Cuts at: 3988 6027 3988 Size: 2039 4436 BlpI GC'TnA_GC Cuts at: 1675 3585 1675 Size: 1910 4565 Bme1580I G_kGCm'C Cuts at: 4098 4191 5249 4098 Size: 93 1058 5324 Fragments arranged by size: 5324 1058 93 BmrI ACTGGGnnnn_n' Cuts at: 2068 3685 3766 4101 6247 2068 Size: 1617 81 335 2146 2296 Fragments arranged by size: 2296 2146 1617 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4840 4840 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 926 1394 2066 2090 926 Size: 468 672 24 5311 Fragments arranged by size: 5311 672 468 24 Bpu10I CC'TnA_GC Cuts at: 1184 2111 1184 Size: 927 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1385 3649 4528 5022 5320 1385 Size: 2264 879 494 298 2540 Fragments arranged by size: 2540 2264 879 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 6179 6179 Size: 6475 BsaAI yAC'GTr Cuts at: 2367 3465 3826 2367 Size: 1098 361 5016 Fragments arranged by size: 5016 1098 361 BsaBI GATnn'nnATC Cuts at: 6 2642 3987 6026 6 Size: 2636 1345 2039 455 Fragments arranged by size: 2636 2039 1345 455 BsaHI Gr'CG_yC Cuts at: 3904 4152 4856 6352 3904 Size: 248 704 1496 4027 Fragments arranged by size: 4027 1496 704 248 BsaWI w'CCGG_w Cuts at: 3662 4183 4863 5137 5284 5695 3662 Size: 521 680 274 147 411 4442 Fragments arranged by size: 4442 680 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 767 770 1172 2307 767 Size: 3 402 1135 4935 Fragments arranged by size: 4935 1135 402 3 BseYI C'CCAG_C Cuts at: 3898 4656 5235 5731 3898 Size: 758 579 496 4642 Fragments arranged by size: 4642 758 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1199 2128 2399 1199 Size: 929 271 5275 Fragments arranged by size: 5275 929 271 BsiEI CG_ry'CG Cuts at: 104 134 4015 4061 5271 104 Size: 30 3881 46 1210 1308 Fragments arranged by size: 3881 1308 1210 46 30 BsiHKAI G_wGCw'C Cuts at: 3487 4265 4455 5249 3487 Size: 778 190 794 4713 Fragments arranged by size: 4713 794 778 190 BsmI GAATG_Cn' Cuts at: 1286 1286 Size: 6475 Bsp1286I G_dGCh'C Cuts at: 3175 3487 4098 4191 4265 4455 4517 5249 3175 Size: 312 611 93 74 190 62 732 4401 Fragments arranged by size: 4401 732 611 312 190 93 74 62 BspEI T'CCGG_A Cuts at: 5695 5695 Size: 6475 BspHI T'CATG_A Cuts at: 237 2301 5651 237 Size: 2064 3350 1061 Fragments arranged by size: 3350 2064 1061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2150 3523 3737 4039 4420 2150 Size: 1373 214 302 381 4205 Fragments arranged by size: 4205 1373 381 302 214 BsrBI CCG'CTC Cuts at: 50 143 4765 4819 6305 6470 50 Size: 93 4622 54 1486 165 55 Fragments arranged by size: 4622 1486 165 93 55 54 BsrDI GCAATG_nn' Cuts at: 1318 2619 4385 1318 Size: 1301 1766 3408 Fragments arranged by size: 3408 1766 1301 BsrFI r'CCGG_y Cuts at: 133 4471 4652 4863 133 Size: 4338 181 211 1745 Fragments arranged by size: 4338 1745 211 181 BsrGI T'GTAC_A Cuts at: 1909 1909 Size: 6475 BssSI C'ACGA_G Cuts at: 4744 5104 4744 Size: 360 6115 BstAPI GCAn_nnn'nTGC Cuts at: 3850 3850 Size: 6475 BstBI TT'CG_AA Cuts at: 110 110 Size: 6475 BstXI CCAn_nnnn'nTGG Cuts at: 2556 2556 Size: 6475 BstYI r'GATC_y Cuts at: 1 3498 3965 3988 4323 4569 5583 5669 5681 Size: 3497 467 23 335 246 1014 86 12 Cuts at: 5681 6027 1 Size: 346 449 Fragments arranged by size: 3497 1014 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1474 1474 Size: 6475 BtgI C'CryG_G Cuts at: 125 125 Size: 6475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2888 4423 4604 4767 2888 Size: 1535 181 163 4596 Fragments arranged by size: 4596 1535 181 163 BtsI GCAGTG_nn' Cuts at: 2697 2961 3716 2697 Size: 264 755 5456 Fragments arranged by size: 5456 755 264 DraI TTT'AAA Cuts at: 216 420 1460 2171 3014 3472 216 Size: 204 1040 711 843 458 3219 Fragments arranged by size: 3219 1040 843 711 458 204 DrdI GACnn_nn'nnGTC Cuts at: 4179 5039 4179 Size: 860 5615 EaeI y'GGCC_r Cuts at: 131 4058 4232 4623 4650 5997 131 Size: 3927 174 391 27 1347 609 Fragments arranged by size: 3927 1347 609 391 174 27 EagI C'GGCC_G Cuts at: 131 4058 131 Size: 3927 2548 EarI CTCTTCn'nnn_ Cuts at: 692 1161 1495 1919 2583 4496 4706 692 Size: 469 334 424 664 1913 210 2461 Fragments arranged by size: 2461 1913 664 469 424 334 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4681 4993 5139 6285 4681 Size: 312 146 1146 4871 Fragments arranged by size: 4871 1146 312 146 EcoICRI GAG'CTC Cuts at: 3485 3485 Size: 6475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 926 1394 2066 2090 2242 2528 2906 3189 3373 Size: 468 672 24 152 286 378 283 184 Cuts at: 3373 4298 4730 5479 5747 926 Size: 925 432 749 268 1654 Fragments arranged by size: 1654 925 749 672 468 432 378 286 283 268 184 152 24 EcoO109I rG'GnC_Cy Cuts at: 2139 3067 3612 2139 Size: 928 545 5002 Fragments arranged by size: 5002 928 545 EcoRI G'AATT_C Cuts at: 373 900 2243 2743 3477 373 Size: 527 1343 500 734 3371 Fragments arranged by size: 3371 1343 734 527 500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4492 4492 Size: 6475 FspI TGC'GCA Cuts at: 4254 4254 Size: 6475 HaeII r_GCGC'y Cuts at: 3907 4155 5179 6158 3907 Size: 248 1024 979 4224 Fragments arranged by size: 4224 1024 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 219 251 691 723 2079 2111 2283 2315 3320 Size: 32 440 32 1356 32 172 32 1005 Cuts at: 3320 3352 3388 3420 4315 4345 4347 4377 219 Size: 32 36 32 895 30 2 30 2317 Fragments arranged by size: 2317 1356 1005 895 440 172 36 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2919 3512 2919 Size: 593 5882 HindIII A'AGCT_T Cuts at: 176 176 Size: 6475 KasI G'GCGC_C Cuts at: 3903 4151 3903 Size: 248 6227 KpnI G_GTAC'C Cuts at: 3493 3493 Size: 6475 MfeI C'AATT_G Cuts at: 440 977 440 Size: 537 5938 MluI A'CGCG_T Cuts at: 5908 5908 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1707 5146 5330 1707 Size: 3439 184 2852 Fragments arranged by size: 3439 2852 184 MscI TGG'CCA Cuts at: 4234 4234 Size: 6475 MslI CAynn'nnrTG Cuts at: 545 2486 2492 2840 4462 4589 545 Size: 1941 6 348 1622 127 2431 Fragments arranged by size: 2431 1941 1622 348 127 6 MspA1I CmG'CkG Cuts at: 3584 3898 4258 5273 5518 3584 Size: 314 360 1015 245 4541 Fragments arranged by size: 4541 1015 360 314 245 NaeI GCC'GGC Cuts at: 135 4654 135 Size: 4519 1956 NarI GG'CG_CC Cuts at: 3904 4152 3904 Size: 248 6227 NcoI C'CATG_G Cuts at: 125 125 Size: 6475 NdeI CA'TA_TG Cuts at: 390 2686 390 Size: 2296 4179 NgoMIV G'CCGG_C Cuts at: 133 4652 133 Size: 4519 1956 NruI TCG'CGA Cuts at: 4836 4836 Size: 6475 NspI r_CATG'y Cuts at: 456 1205 3526 4026 4935 456 Size: 749 2321 500 909 1996 Fragments arranged by size: 2321 1996 909 749 500 PciI A'CATG_T Cuts at: 452 4931 452 Size: 4479 1996 PflMI CCAn_nnn'nTGG Cuts at: 1225 1225 Size: 6475 PmeI GTTT'AAAC Cuts at: 3472 3472 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5614 5646 5614 Size: 32 6443 PpuMI rG'GwC_Cy Cuts at: 2139 2139 Size: 6475 PshAI GACnn'nnGTC Cuts at: 915 915 Size: 6475 PsiI TTA'TAA Cuts at: 2675 2675 Size: 6475 PstI C_TGCA'G Cuts at: 2989 3520 4205 2989 Size: 531 685 5259 Fragments arranged by size: 5259 685 531 PvuI CG_AT'CG Cuts at: 104 104 Size: 6475 PvuII CAG'CTG Cuts at: 3898 4258 3898 Size: 360 6115 RsrII CG'GwC_CG Cuts at: 4668 4668 Size: 6475 SacI G_AGCT'C Cuts at: 3487 3487 Size: 6475 SalI G'TCGA_C Cuts at: 2917 3510 2917 Size: 593 5882 SapI GCTCTTCn'nnn_ Cuts at: 4496 4706 4496 Size: 210 6265 SbfI CC_TGCA'GG Cuts at: 3520 3520 Size: 6475 ScaI AGT'ACT Cuts at: 5992 5992 Size: 6475 SfcI C'TryA_G Cuts at: 33 515 756 1568 1634 2519 2806 2868 2985 Size: 482 241 812 66 885 287 62 117 Cuts at: 2985 3358 3516 4201 5196 5387 33 Size: 373 158 685 995 191 1121 Fragments arranged by size: 1121 995 885 812 685 482 373 287 241 191 158 117 66 62 SfoI GGC'GCC Cuts at: 3905 4153 3905 Size: 248 6227 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6475 SmaI CCC'GGG Cuts at: 3495 3495 Size: 6475 SmlI C'TyrA_G Cuts at: 1400 2847 3628 4543 5037 5299 1400 Size: 1447 781 915 494 262 2576 Fragments arranged by size: 2576 1447 915 781 494 262 SnaBI TAC'GTA Cuts at: 3465 3465 Size: 6475 SpeI A'CTAG_T Cuts at: 67 67 Size: 6475 SphI G_CATG'C Cuts at: 3526 4026 3526 Size: 500 5975 StyI C'CwwG_G Cuts at: 125 666 3607 3961 125 Size: 541 2941 354 2639 Fragments arranged by size: 2941 2639 541 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 608 2255 3065 4056 4189 4648 4859 608 Size: 1647 810 991 133 459 211 2224 Fragments arranged by size: 2224 1647 991 810 459 211 133 TatI w'GTAC_w Cuts at: 738 1909 5990 738 Size: 1171 4081 1223 Fragments arranged by size: 4081 1223 1171 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 608 640 2778 2810 5992 6024 608 Size: 32 2138 32 3182 32 1059 Fragments arranged by size: 3182 2138 1059 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1450 4270 1450 Size: 2820 3655 XbaI T'CTAG_A Cuts at: 3504 3504 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3968 3968 Size: 6475 XmaI C'CCGG_G Cuts at: 3493 3493 Size: 6475 XmnI GAAnn'nnTTC Cuts at: 971 3150 971 Size: 2179 4296 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AhdI AleI ApaI AscI AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NheI NotI NsiI PacI PasI PfoI PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI