Restriction map for
pF1KSDA0797
(Circular) (Six-base) MAPSORT of: pF1KSDA0797.seq Check: 6910 from: 1 to: 6475
pF1KSDA0797 6475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 528 865 1473 2918 3511 528
Size: 337 608 1445 593 3492
Fragments arranged by size:
3492 1445 608 593 337
Acc65I G'GTAC_C
Cuts at: 3489 3489
Size: 6475
AclI AA'CG_TT
Cuts at: 6315 6315
Size: 6475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 2528 2906 3189 3373 4298 4730 5479 5747
Size: 286 378 283 184 925 432 749 268
Cuts at: 5747 2242
Size: 2970
Fragments arranged by size:
2970 925 749 432 378 286 283 268
184
AflII C'TTAA_G
Cuts at: 2847 2847
Size: 6475
AflIII A'CryG_T
Cuts at: 452 3825 4931 5908 452
Size: 3373 1106 977 1019
Fragments arranged by size:
3373 1106 1019 977
AgeI A'CCGG_T
Cuts at: 4863 4863
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1702 1702
Size: 6475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 503 535 503
Size: 32 6443
AlwNI CAG_nnn'CTG
Cuts at: 3212 3778 5347 3212
Size: 566 1569 4340
Fragments arranged by size:
4340 1569 566
ApaLI G'TGCA_C
Cuts at: 5245 5245
Size: 6475
ApoI r'AATT_y
Cuts at: 373 900 1746 1871 2014 2164 2243 2743 3092
Size: 527 846 125 143 150 79 500 349
Cuts at: 3092 3285 3477 5828 6084 373
Size: 193 192 2351 256 764
Fragments arranged by size:
2351 846 764 527 500 349 256 193
192 150 143 125 79
AseI AT'TA_AT
Cuts at: 20 641 1826 20
Size: 621 1185 4669
Fragments arranged by size:
4669 1185 621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6475
AvaI C'yCGr_G
Cuts at: 3493 3493
Size: 6475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 744 777 744
Size: 33 6442
BamHI G'GATC_C
Cuts at: 3498 3498
Size: 6475
BanI G'GyrC_C
Cuts at: 3489 3903 4151 4186 3489
Size: 414 248 35 5778
Fragments arranged by size:
5778 414 248 35
BanII G_rGCy'C
Cuts at: 3175 3487 4517 3175
Size: 312 1030 5133
Fragments arranged by size:
5133 1030 312
BbeI G_GCGC'C
Cuts at: 3907 4155 3907
Size: 248 6227
BbsI GAAGACnn'nnnn_
Cuts at: 391 1301 2237 391
Size: 910 936 4629
Fragments arranged by size:
4629 936 910
BbvCI CC'TCA_GC
Cuts at: 1184 1184
Size: 6475
BciVI GTATCCnnnnn_n'
Cuts at: 3052 4371 5134 6475 3052
Size: 1319 763 1341 3052
Fragments arranged by size:
3052 1341 1319 763
BclI T'GATC_A
Cuts at: 234 3993 234
Size: 3759 2716
BglII A'GATC_T
Cuts at: 3988 6027 3988
Size: 2039 4436
BlpI GC'TnA_GC
Cuts at: 1675 3585 1675
Size: 1910 4565
Bme1580I G_kGCm'C
Cuts at: 4098 4191 5249 4098
Size: 93 1058 5324
Fragments arranged by size:
5324 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2068 3685 3766 4101 6247 2068
Size: 1617 81 335 2146 2296
Fragments arranged by size:
2296 2146 1617 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4840 4840
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 1394 2066 2090 926
Size: 468 672 24 5311
Fragments arranged by size:
5311 672 468 24
Bpu10I CC'TnA_GC
Cuts at: 1184 2111 1184
Size: 927 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 3649 4528 5022 5320 1385
Size: 2264 879 494 298 2540
Fragments arranged by size:
2540 2264 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6179 6179
Size: 6475
BsaAI yAC'GTr
Cuts at: 2367 3465 3826 2367
Size: 1098 361 5016
Fragments arranged by size:
5016 1098 361
BsaBI GATnn'nnATC
Cuts at: 6 2642 3987 6026 6
Size: 2636 1345 2039 455
Fragments arranged by size:
2636 2039 1345 455
BsaHI Gr'CG_yC
Cuts at: 3904 4152 4856 6352 3904
Size: 248 704 1496 4027
Fragments arranged by size:
4027 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3662 4183 4863 5137 5284 5695 3662
Size: 521 680 274 147 411 4442
Fragments arranged by size:
4442 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 767 770 1172 2307 767
Size: 3 402 1135 4935
Fragments arranged by size:
4935 1135 402 3
BseYI C'CCAG_C
Cuts at: 3898 4656 5235 5731 3898
Size: 758 579 496 4642
Fragments arranged by size:
4642 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 2128 2399 1199
Size: 929 271 5275
Fragments arranged by size:
5275 929 271
BsiEI CG_ry'CG
Cuts at: 104 134 4015 4061 5271 104
Size: 30 3881 46 1210 1308
Fragments arranged by size:
3881 1308 1210 46 30
BsiHKAI G_wGCw'C
Cuts at: 3487 4265 4455 5249 3487
Size: 778 190 794 4713
Fragments arranged by size:
4713 794 778 190
BsmI GAATG_Cn'
Cuts at: 1286 1286
Size: 6475
Bsp1286I G_dGCh'C
Cuts at: 3175 3487 4098 4191 4265 4455 4517 5249 3175
Size: 312 611 93 74 190 62 732 4401
Fragments arranged by size:
4401 732 611 312 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5695 5695
Size: 6475
BspHI T'CATG_A
Cuts at: 237 2301 5651 237
Size: 2064 3350 1061
Fragments arranged by size:
3350 2064 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2150 3523 3737 4039 4420 2150
Size: 1373 214 302 381 4205
Fragments arranged by size:
4205 1373 381 302 214
BsrBI CCG'CTC
Cuts at: 50 143 4765 4819 6305 6470 50
Size: 93 4622 54 1486 165 55
Fragments arranged by size:
4622 1486 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 1318 2619 4385 1318
Size: 1301 1766 3408
Fragments arranged by size:
3408 1766 1301
BsrFI r'CCGG_y
Cuts at: 133 4471 4652 4863 133
Size: 4338 181 211 1745
Fragments arranged by size:
4338 1745 211 181
BsrGI T'GTAC_A
Cuts at: 1909 1909
Size: 6475
BssSI C'ACGA_G
Cuts at: 4744 5104 4744
Size: 360 6115
BstAPI GCAn_nnn'nTGC
Cuts at: 3850 3850
Size: 6475
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6475
BstXI CCAn_nnnn'nTGG
Cuts at: 2556 2556
Size: 6475
BstYI r'GATC_y
Cuts at: 1 3498 3965 3988 4323 4569 5583 5669 5681
Size: 3497 467 23 335 246 1014 86 12
Cuts at: 5681 6027 1
Size: 346 449
Fragments arranged by size:
3497 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1474 1474
Size: 6475
BtgI C'CryG_G
Cuts at: 125 125
Size: 6475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2888 4423 4604 4767 2888
Size: 1535 181 163 4596
Fragments arranged by size:
4596 1535 181 163
BtsI GCAGTG_nn'
Cuts at: 2697 2961 3716 2697
Size: 264 755 5456
Fragments arranged by size:
5456 755 264
DraI TTT'AAA
Cuts at: 216 420 1460 2171 3014 3472 216
Size: 204 1040 711 843 458 3219
Fragments arranged by size:
3219 1040 843 711 458 204
DrdI GACnn_nn'nnGTC
Cuts at: 4179 5039 4179
Size: 860 5615
EaeI y'GGCC_r
Cuts at: 131 4058 4232 4623 4650 5997 131
Size: 3927 174 391 27 1347 609
Fragments arranged by size:
3927 1347 609 391 174 27
EagI C'GGCC_G
Cuts at: 131 4058 131
Size: 3927 2548
EarI CTCTTCn'nnn_
Cuts at: 692 1161 1495 1919 2583 4496 4706 692
Size: 469 334 424 664 1913 210 2461
Fragments arranged by size:
2461 1913 664 469 424 334 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4681 4993 5139 6285 4681
Size: 312 146 1146 4871
Fragments arranged by size:
4871 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3485 3485
Size: 6475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 1394 2066 2090 2242 2528 2906 3189 3373
Size: 468 672 24 152 286 378 283 184
Cuts at: 3373 4298 4730 5479 5747 926
Size: 925 432 749 268 1654
Fragments arranged by size:
1654 925 749 672 468 432 378 286
283 268 184 152 24
EcoO109I rG'GnC_Cy
Cuts at: 2139 3067 3612 2139
Size: 928 545 5002
Fragments arranged by size:
5002 928 545
EcoRI G'AATT_C
Cuts at: 373 900 2243 2743 3477 373
Size: 527 1343 500 734 3371
Fragments arranged by size:
3371 1343 734 527 500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4492 4492
Size: 6475
FspI TGC'GCA
Cuts at: 4254 4254
Size: 6475
HaeII r_GCGC'y
Cuts at: 3907 4155 5179 6158 3907
Size: 248 1024 979 4224
Fragments arranged by size:
4224 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 251 691 723 2079 2111 2283 2315 3320
Size: 32 440 32 1356 32 172 32 1005
Cuts at: 3320 3352 3388 3420 4315 4345 4347 4377 219
Size: 32 36 32 895 30 2 30 2317
Fragments arranged by size:
2317 1356 1005 895 440 172 36 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2919 3512 2919
Size: 593 5882
HindIII A'AGCT_T
Cuts at: 176 176
Size: 6475
KasI G'GCGC_C
Cuts at: 3903 4151 3903
Size: 248 6227
KpnI G_GTAC'C
Cuts at: 3493 3493
Size: 6475
MfeI C'AATT_G
Cuts at: 440 977 440
Size: 537 5938
MluI A'CGCG_T
Cuts at: 5908 5908
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1707 5146 5330 1707
Size: 3439 184 2852
Fragments arranged by size:
3439 2852 184
MscI TGG'CCA
Cuts at: 4234 4234
Size: 6475
MslI CAynn'nnrTG
Cuts at: 545 2486 2492 2840 4462 4589 545
Size: 1941 6 348 1622 127 2431
Fragments arranged by size:
2431 1941 1622 348 127 6
MspA1I CmG'CkG
Cuts at: 3584 3898 4258 5273 5518 3584
Size: 314 360 1015 245 4541
Fragments arranged by size:
4541 1015 360 314 245
NaeI GCC'GGC
Cuts at: 135 4654 135
Size: 4519 1956
NarI GG'CG_CC
Cuts at: 3904 4152 3904
Size: 248 6227
NcoI C'CATG_G
Cuts at: 125 125
Size: 6475
NdeI CA'TA_TG
Cuts at: 390 2686 390
Size: 2296 4179
NgoMIV G'CCGG_C
Cuts at: 133 4652 133
Size: 4519 1956
NruI TCG'CGA
Cuts at: 4836 4836
Size: 6475
NspI r_CATG'y
Cuts at: 456 1205 3526 4026 4935 456
Size: 749 2321 500 909 1996
Fragments arranged by size:
2321 1996 909 749 500
PciI A'CATG_T
Cuts at: 452 4931 452
Size: 4479 1996
PflMI CCAn_nnn'nTGG
Cuts at: 1225 1225
Size: 6475
PmeI GTTT'AAAC
Cuts at: 3472 3472
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5614 5646 5614
Size: 32 6443
PpuMI rG'GwC_Cy
Cuts at: 2139 2139
Size: 6475
PshAI GACnn'nnGTC
Cuts at: 915 915
Size: 6475
PsiI TTA'TAA
Cuts at: 2675 2675
Size: 6475
PstI C_TGCA'G
Cuts at: 2989 3520 4205 2989
Size: 531 685 5259
Fragments arranged by size:
5259 685 531
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6475
PvuII CAG'CTG
Cuts at: 3898 4258 3898
Size: 360 6115
RsrII CG'GwC_CG
Cuts at: 4668 4668
Size: 6475
SacI G_AGCT'C
Cuts at: 3487 3487
Size: 6475
SalI G'TCGA_C
Cuts at: 2917 3510 2917
Size: 593 5882
SapI GCTCTTCn'nnn_
Cuts at: 4496 4706 4496
Size: 210 6265
SbfI CC_TGCA'GG
Cuts at: 3520 3520
Size: 6475
ScaI AGT'ACT
Cuts at: 5992 5992
Size: 6475
SfcI C'TryA_G
Cuts at: 33 515 756 1568 1634 2519 2806 2868 2985
Size: 482 241 812 66 885 287 62 117
Cuts at: 2985 3358 3516 4201 5196 5387 33
Size: 373 158 685 995 191 1121
Fragments arranged by size:
1121 995 885 812 685 482 373 287
241 191 158 117 66 62
SfoI GGC'GCC
Cuts at: 3905 4153 3905
Size: 248 6227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6475
SmaI CCC'GGG
Cuts at: 3495 3495
Size: 6475
SmlI C'TyrA_G
Cuts at: 1400 2847 3628 4543 5037 5299 1400
Size: 1447 781 915 494 262 2576
Fragments arranged by size:
2576 1447 915 781 494 262
SnaBI TAC'GTA
Cuts at: 3465 3465
Size: 6475
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6475
SphI G_CATG'C
Cuts at: 3526 4026 3526
Size: 500 5975
StyI C'CwwG_G
Cuts at: 125 666 3607 3961 125
Size: 541 2941 354 2639
Fragments arranged by size:
2941 2639 541 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 608 2255 3065 4056 4189 4648 4859 608
Size: 1647 810 991 133 459 211 2224
Fragments arranged by size:
2224 1647 991 810 459 211 133
TatI w'GTAC_w
Cuts at: 738 1909 5990 738
Size: 1171 4081 1223
Fragments arranged by size:
4081 1223 1171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 608 640 2778 2810 5992 6024 608
Size: 32 2138 32 3182 32 1059
Fragments arranged by size:
3182 2138 1059 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1450 4270 1450
Size: 2820 3655
XbaI T'CTAG_A
Cuts at: 3504 3504
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3968 3968
Size: 6475
XmaI C'CCGG_G
Cuts at: 3493 3493
Size: 6475
XmnI GAAnn'nnTTC
Cuts at: 971 3150 971
Size: 2179 4296
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NsiI PacI PasI
PfoI PmlI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI