Restriction map for pF1KSDA0797
 (Circular) (Six-base) MAPSORT of: pF1KSDA0797.seq  Check: 6910  from: 1  to: 6475



pF1KSDA0797  6475 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:    528     865    1473    2918    3511     528 
   Size:        337     608    1445     593    3492

  Fragments arranged by size: 

                3492    1445     608     593     337
 
Acc65I G'GTAC_C

Cuts at:   3489    3489 
   Size:       6475
 
AclI AA'CG_TT

Cuts at:   6315    6315 
   Size:       6475
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2242    2528    2906    3189    3373    4298    4730    5479    5747 
   Size:        286     378     283     184     925     432     749     268

Cuts at:   5747    2242 
   Size:       2970

  Fragments arranged by size: 

                2970     925     749     432     378     286     283     268
                 184
 
AflII C'TTAA_G

Cuts at:   2847    2847 
   Size:       6475
 
AflIII A'CryG_T

Cuts at:    452    3825    4931    5908     452 
   Size:       3373    1106     977    1019

  Fragments arranged by size: 

                3373    1106    1019     977
 
AgeI A'CCGG_T

Cuts at:   4863    4863 
   Size:       6475
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1702    1702 
   Size:       6475
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    503     535     503 
   Size:         32    6443
 
AlwNI CAG_nnn'CTG

Cuts at:   3212    3778    5347    3212 
   Size:        566    1569    4340

  Fragments arranged by size: 

                4340    1569     566
 
ApaLI G'TGCA_C

Cuts at:   5245    5245 
   Size:       6475
 
ApoI r'AATT_y

Cuts at:    373     900    1746    1871    2014    2164    2243    2743    3092 
   Size:        527     846     125     143     150      79     500     349

Cuts at:   3092    3285    3477    5828    6084     373 
   Size:        193     192    2351     256     764

  Fragments arranged by size: 

                2351     846     764     527     500     349     256     193
                 192     150     143     125      79
 
AseI AT'TA_AT

Cuts at:     20     641    1826      20 
   Size:        621    1185    4669

  Fragments arranged by size: 

                4669    1185     621
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6475
 
AvaI C'yCGr_G

Cuts at:   3493    3493 
   Size:       6475
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    744     777     744 
   Size:         33    6442
 
BamHI G'GATC_C

Cuts at:   3498    3498 
   Size:       6475
 
BanI G'GyrC_C

Cuts at:   3489    3903    4151    4186    3489 
   Size:        414     248      35    5778

  Fragments arranged by size: 

                5778     414     248      35
 
BanII G_rGCy'C

Cuts at:   3175    3487    4517    3175 
   Size:        312    1030    5133

  Fragments arranged by size: 

                5133    1030     312
 
BbeI G_GCGC'C

Cuts at:   3907    4155    3907 
   Size:        248    6227
 
BbsI GAAGACnn'nnnn_

Cuts at:    391    1301    2237     391 
   Size:        910     936    4629

  Fragments arranged by size: 

                4629     936     910
 
BbvCI CC'TCA_GC

Cuts at:   1184    1184 
   Size:       6475
 
BciVI GTATCCnnnnn_n'

Cuts at:   3052    4371    5134    6475    3052 
   Size:       1319     763    1341    3052

  Fragments arranged by size: 

                3052    1341    1319     763
 
BclI T'GATC_A

Cuts at:    234    3993     234 
   Size:       3759    2716
 
BglII A'GATC_T

Cuts at:   3988    6027    3988 
   Size:       2039    4436
 
BlpI GC'TnA_GC

Cuts at:   1675    3585    1675 
   Size:       1910    4565
 
Bme1580I G_kGCm'C

Cuts at:   4098    4191    5249    4098 
   Size:         93    1058    5324

  Fragments arranged by size: 

                5324    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2068    3685    3766    4101    6247    2068 
   Size:       1617      81     335    2146    2296

  Fragments arranged by size: 

                2296    2146    1617     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4840    4840 
   Size:       6475
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    926    1394    2066    2090     926 
   Size:        468     672      24    5311

  Fragments arranged by size: 

                5311     672     468      24
 
Bpu10I CC'TnA_GC

Cuts at:   1184    2111    1184 
   Size:        927    5548
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1385    3649    4528    5022    5320    1385 
   Size:       2264     879     494     298    2540

  Fragments arranged by size: 

                2540    2264     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   6179    6179 
   Size:       6475
 
BsaAI yAC'GTr

Cuts at:   2367    3465    3826    2367 
   Size:       1098     361    5016

  Fragments arranged by size: 

                5016    1098     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2642    3987    6026       6 
   Size:       2636    1345    2039     455

  Fragments arranged by size: 

                2636    2039    1345     455
 
BsaHI Gr'CG_yC

Cuts at:   3904    4152    4856    6352    3904 
   Size:        248     704    1496    4027

  Fragments arranged by size: 

                4027    1496     704     248
 
BsaWI w'CCGG_w

Cuts at:   3662    4183    4863    5137    5284    5695    3662 
   Size:        521     680     274     147     411    4442

  Fragments arranged by size: 

                4442     680     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    767     770    1172    2307     767 
   Size:          3     402    1135    4935

  Fragments arranged by size: 

                4935    1135     402       3
 
BseYI C'CCAG_C

Cuts at:   3898    4656    5235    5731    3898 
   Size:        758     579     496    4642

  Fragments arranged by size: 

                4642     758     579     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1199    2128    2399    1199 
   Size:        929     271    5275

  Fragments arranged by size: 

                5275     929     271
 
BsiEI CG_ry'CG

Cuts at:    104     134    4015    4061    5271     104 
   Size:         30    3881      46    1210    1308

  Fragments arranged by size: 

                3881    1308    1210      46      30
 
BsiHKAI G_wGCw'C

Cuts at:   3487    4265    4455    5249    3487 
   Size:        778     190     794    4713

  Fragments arranged by size: 

                4713     794     778     190
 
BsmI GAATG_Cn'

Cuts at:   1286    1286 
   Size:       6475
 
Bsp1286I G_dGCh'C

Cuts at:   3175    3487    4098    4191    4265    4455    4517    5249    3175 
   Size:        312     611      93      74     190      62     732    4401

  Fragments arranged by size: 

                4401     732     611     312     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   5695    5695 
   Size:       6475
 
BspHI T'CATG_A

Cuts at:    237    2301    5651     237 
   Size:       2064    3350    1061

  Fragments arranged by size: 

                3350    2064    1061
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2150    3523    3737    4039    4420    2150 
   Size:       1373     214     302     381    4205

  Fragments arranged by size: 

                4205    1373     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     143    4765    4819    6305    6470      50 
   Size:         93    4622      54    1486     165      55

  Fragments arranged by size: 

                4622    1486     165      93      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1318    2619    4385    1318 
   Size:       1301    1766    3408

  Fragments arranged by size: 

                3408    1766    1301
 
BsrFI r'CCGG_y

Cuts at:    133    4471    4652    4863     133 
   Size:       4338     181     211    1745

  Fragments arranged by size: 

                4338    1745     211     181
 
BsrGI T'GTAC_A

Cuts at:   1909    1909 
   Size:       6475
 
BssSI C'ACGA_G

Cuts at:   4744    5104    4744 
   Size:        360    6115
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3850    3850 
   Size:       6475
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       6475
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2556    2556 
   Size:       6475
 
BstYI r'GATC_y

Cuts at:      1    3498    3965    3988    4323    4569    5583    5669    5681 
   Size:       3497     467      23     335     246    1014      86      12

Cuts at:   5681    6027       1 
   Size:        346     449

  Fragments arranged by size: 

                3497    1014     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:   1474    1474 
   Size:       6475
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       6475
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2888    4423    4604    4767    2888 
   Size:       1535     181     163    4596

  Fragments arranged by size: 

                4596    1535     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2697    2961    3716    2697 
   Size:        264     755    5456

  Fragments arranged by size: 

                5456     755     264
 
DraI TTT'AAA

Cuts at:    216     420    1460    2171    3014    3472     216 
   Size:        204    1040     711     843     458    3219

  Fragments arranged by size: 

                3219    1040     843     711     458     204
 
DrdI GACnn_nn'nnGTC

Cuts at:   4179    5039    4179 
   Size:        860    5615
 
EaeI y'GGCC_r

Cuts at:    131    4058    4232    4623    4650    5997     131 
   Size:       3927     174     391      27    1347     609

  Fragments arranged by size: 

                3927    1347     609     391     174      27
 
EagI C'GGCC_G

Cuts at:    131    4058     131 
   Size:       3927    2548
 
EarI CTCTTCn'nnn_

Cuts at:    692    1161    1495    1919    2583    4496    4706     692 
   Size:        469     334     424     664    1913     210    2461

  Fragments arranged by size: 

                2461    1913     664     469     424     334     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4681    4993    5139    6285    4681 
   Size:        312     146    1146    4871

  Fragments arranged by size: 

                4871    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   3485    3485 
   Size:       6475
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    926    1394    2066    2090    2242    2528    2906    3189    3373 
   Size:        468     672      24     152     286     378     283     184

Cuts at:   3373    4298    4730    5479    5747     926 
   Size:        925     432     749     268    1654

  Fragments arranged by size: 

                1654     925     749     672     468     432     378     286
                 283     268     184     152      24
 
EcoO109I rG'GnC_Cy

Cuts at:   2139    3067    3612    2139 
   Size:        928     545    5002

  Fragments arranged by size: 

                5002     928     545
 
EcoRI G'AATT_C

Cuts at:    373     900    2243    2743    3477     373 
   Size:        527    1343     500     734    3371

  Fragments arranged by size: 

                3371    1343     734     527     500
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4492    4492 
   Size:       6475
 
FspI TGC'GCA

Cuts at:   4254    4254 
   Size:       6475
 
HaeII r_GCGC'y

Cuts at:   3907    4155    5179    6158    3907 
   Size:        248    1024     979    4224

  Fragments arranged by size: 

                4224    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    219     251     691     723    2079    2111    2283    2315    3320 
   Size:         32     440      32    1356      32     172      32    1005

Cuts at:   3320    3352    3388    3420    4315    4345    4347    4377     219 
   Size:         32      36      32     895      30       2      30    2317

  Fragments arranged by size: 

                2317    1356    1005     895     440     172      36      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2919    3512    2919 
   Size:        593    5882
 
HindIII A'AGCT_T

Cuts at:    176     176 
   Size:       6475
 
KasI G'GCGC_C

Cuts at:   3903    4151    3903 
   Size:        248    6227
 
KpnI G_GTAC'C

Cuts at:   3493    3493 
   Size:       6475
 
MfeI C'AATT_G

Cuts at:    440     977     440 
   Size:        537    5938
 
MluI A'CGCG_T

Cuts at:   5908    5908 
   Size:       6475
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1707    5146    5330    1707 
   Size:       3439     184    2852

  Fragments arranged by size: 

                3439    2852     184
 
MscI TGG'CCA

Cuts at:   4234    4234 
   Size:       6475
 
MslI CAynn'nnrTG

Cuts at:    545    2486    2492    2840    4462    4589     545 
   Size:       1941       6     348    1622     127    2431

  Fragments arranged by size: 

                2431    1941    1622     348     127       6
 
MspA1I CmG'CkG

Cuts at:   3584    3898    4258    5273    5518    3584 
   Size:        314     360    1015     245    4541

  Fragments arranged by size: 

                4541    1015     360     314     245
 
NaeI GCC'GGC

Cuts at:    135    4654     135 
   Size:       4519    1956
 
NarI GG'CG_CC

Cuts at:   3904    4152    3904 
   Size:        248    6227
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       6475
 
NdeI CA'TA_TG

Cuts at:    390    2686     390 
   Size:       2296    4179
 
NgoMIV G'CCGG_C

Cuts at:    133    4652     133 
   Size:       4519    1956
 
NruI TCG'CGA

Cuts at:   4836    4836 
   Size:       6475
 
NspI r_CATG'y

Cuts at:    456    1205    3526    4026    4935     456 
   Size:        749    2321     500     909    1996

  Fragments arranged by size: 

                2321    1996     909     749     500
 
PciI A'CATG_T

Cuts at:    452    4931     452 
   Size:       4479    1996
 
PflMI CCAn_nnn'nTGG

Cuts at:   1225    1225 
   Size:       6475
 
PmeI GTTT'AAAC

Cuts at:   3472    3472 
   Size:       6475
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5614    5646    5614 
   Size:         32    6443
 
PpuMI rG'GwC_Cy

Cuts at:   2139    2139 
   Size:       6475
 
PshAI GACnn'nnGTC

Cuts at:    915     915 
   Size:       6475
 
PsiI TTA'TAA

Cuts at:   2675    2675 
   Size:       6475
 
PstI C_TGCA'G

Cuts at:   2989    3520    4205    2989 
   Size:        531     685    5259

  Fragments arranged by size: 

                5259     685     531
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6475
 
PvuII CAG'CTG

Cuts at:   3898    4258    3898 
   Size:        360    6115
 
RsrII CG'GwC_CG

Cuts at:   4668    4668 
   Size:       6475
 
SacI G_AGCT'C

Cuts at:   3487    3487 
   Size:       6475
 
SalI G'TCGA_C

Cuts at:   2917    3510    2917 
   Size:        593    5882
 
SapI GCTCTTCn'nnn_

Cuts at:   4496    4706    4496 
   Size:        210    6265
 
SbfI CC_TGCA'GG

Cuts at:   3520    3520 
   Size:       6475
 
ScaI AGT'ACT

Cuts at:   5992    5992 
   Size:       6475
 
SfcI C'TryA_G

Cuts at:     33     515     756    1568    1634    2519    2806    2868    2985 
   Size:        482     241     812      66     885     287      62     117

Cuts at:   2985    3358    3516    4201    5196    5387      33 
   Size:        373     158     685     995     191    1121

  Fragments arranged by size: 

                1121     995     885     812     685     482     373     287
                 241     191     158     117      66      62
 
SfoI GGC'GCC

Cuts at:   3905    4153    3905 
   Size:        248    6227
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6475
 
SmaI CCC'GGG

Cuts at:   3495    3495 
   Size:       6475
 
SmlI C'TyrA_G

Cuts at:   1400    2847    3628    4543    5037    5299    1400 
   Size:       1447     781     915     494     262    2576

  Fragments arranged by size: 

                2576    1447     915     781     494     262
 
SnaBI TAC'GTA

Cuts at:   3465    3465 
   Size:       6475
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6475
 
SphI G_CATG'C

Cuts at:   3526    4026    3526 
   Size:        500    5975
 
StyI C'CwwG_G

Cuts at:    125     666    3607    3961     125 
   Size:        541    2941     354    2639

  Fragments arranged by size: 

                2941    2639     541     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    608    2255    3065    4056    4189    4648    4859     608 
   Size:       1647     810     991     133     459     211    2224

  Fragments arranged by size: 

                2224    1647     991     810     459     211     133
 
TatI w'GTAC_w

Cuts at:    738    1909    5990     738 
   Size:       1171    4081    1223

  Fragments arranged by size: 

                4081    1223    1171
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    608     640    2778    2810    5992    6024     608 
   Size:         32    2138      32    3182      32    1059

  Fragments arranged by size: 

                3182    2138    1059      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1450    4270    1450 
   Size:       2820    3655
 
XbaI T'CTAG_A

Cuts at:   3504    3504 
   Size:       6475
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3968    3968 
   Size:       6475
 
XmaI C'CCGG_G

Cuts at:   3493    3493 
   Size:       6475
 
XmnI GAAnn'nnTTC

Cuts at:    971    3150     971 
   Size:       2179    4296
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NruI     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     ApaI     AscI    AvrII
     BcgI    BfrBI     BglI    BmgBI     BmtI    BsaXI    BsiWI    BsmBI
   BssHII   BstEII   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI     HpaI     NheI     NotI     NsiI     PacI     PasI
     PfoI     PmlI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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