Restriction map for
pF1KSDA0798
(Circular) (Six-base) MAPSORT of: pF1KSDA0798.seq Check: 9616 from: 1 to: 5093
pF1KSDA0798 5093 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2131 2131
Size: 5093
Acc65I G'GTAC_C
Cuts at: 2109 2109
Size: 5093
AclI AA'CG_TT
Cuts at: 4933 4933
Size: 5093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 2918 3350 4097 4365 439
Size: 2479 432 747 268 1167
Fragments arranged by size:
2479 1167 747 432 268
AflIII A'CryG_T
Cuts at: 2445 3549 4526 2445
Size: 1104 977 3012
Fragments arranged by size:
3012 1104 977
AgeI A'CCGG_T
Cuts at: 3481 3481
Size: 5093
AlwNI CAG_nnn'CTG
Cuts at: 198 2398 3965 198
Size: 2200 1567 1326
Fragments arranged by size:
2200 1567 1326
ApaI G_GGCC'C
Cuts at: 205 205
Size: 5093
ApaLI G'TGCA_C
Cuts at: 3863 3863
Size: 5093
ApoI r'AATT_y
Cuts at: 543 595 658 884 1808 2097 4446 4702 543
Size: 52 63 226 924 289 2349 256 934
Fragments arranged by size:
2349 934 924 289 256 226 63 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 5093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 2113 2113
Size: 5093
BamHI G'GATC_C
Cuts at: 2118 2118
Size: 5093
BanI G'GyrC_C
Cuts at: 2109 2523 2771 2806 2109
Size: 414 248 35 4396
Fragments arranged by size:
4396 414 248 35
BanII G_rGCy'C
Cuts at: 205 2107 3137 205
Size: 1902 1030 2161
Fragments arranged by size:
2161 1902 1030
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 924 924
Size: 5093
BciVI GTATCCnnnnn_n'
Cuts at: 2991 3752 5093 2991
Size: 761 1341 2991
Fragments arranged by size:
2991 1341 761
BclI T'GATC_A
Cuts at: 128 381 789 2613 128
Size: 253 408 1824 2608
Fragments arranged by size:
2608 1824 408 253
BfrBI ATG'CAT
Cuts at: 451 451
Size: 5093
BglII A'GATC_T
Cuts at: 242 2608 4645 242
Size: 2366 2037 690
Fragments arranged by size:
2366 2037 690
BlpI GC'TnA_GC
Cuts at: 2205 2205
Size: 5093
Bme1580I G_kGCm'C
Cuts at: 205 2718 2811 3867 205
Size: 2513 93 1056 1431
Fragments arranged by size:
2513 1431 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1115 2305 2386 2721 4865 1115
Size: 1190 81 335 2144 1343
Fragments arranged by size:
2144 1343 1190 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 837 998 1082 1586 1670 1754 1922 175
Size: 662 161 84 504 84 84 168 3346
Fragments arranged by size:
3346 662 504 168 161 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3148 3640 3938 2269
Size: 879 492 298 3424
Fragments arranged by size:
3424 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4797 4797
Size: 5093
BsaAI yAC'GTr
Cuts at: 741 2085 2446 741
Size: 1344 361 3388
Fragments arranged by size:
3388 1344 361
BsaBI GATnn'nnATC
Cuts at: 6 383 2607 4644 6
Size: 377 2224 2037 455
Fragments arranged by size:
2224 2037 455 377
BsaHI Gr'CG_yC
Cuts at: 2524 2772 3474 4970 2524
Size: 248 702 1496 2647
Fragments arranged by size:
2647 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2282 2803 3481 3755 3902 4313 2282
Size: 521 678 274 147 411 3062
Fragments arranged by size:
3062 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 326 2019 199
Size: 127 1693 3273
Fragments arranged by size:
3273 1693 127
BseYI C'CCAG_C
Cuts at: 2518 3276 3853 4349 2518
Size: 758 577 496 3262
Fragments arranged by size:
3262 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1605 1773 1605
Size: 168 4925
BsiEI CG_ry'CG
Cuts at: 104 2635 2681 3889 104
Size: 2531 46 1208 1308
Fragments arranged by size:
2531 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2107 2885 3075 3867 2107
Size: 778 190 792 3333
Fragments arranged by size:
3333 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 205 2107 2718 2811 2885 3075 3137 3867 205
Size: 1902 611 93 74 190 62 730 1431
Fragments arranged by size:
1902 1431 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4313 4313
Size: 5093
BspHI T'CATG_A
Cuts at: 504 720 792 4269 504
Size: 216 72 3477 1328
Fragments arranged by size:
3477 1328 216 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 2143 2357 2659 3040 263
Size: 1880 214 302 381 2316
Fragments arranged by size:
2316 1880 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3385 3439 4923 5088 50
Size: 3335 54 1484 165 55
Fragments arranged by size:
3335 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1005 1257 1677 3005 1005
Size: 252 420 1328 3093
Fragments arranged by size:
3093 1328 420 252
BsrFI r'CCGG_y
Cuts at: 3091 3272 3481 3091
Size: 181 209 4703
Fragments arranged by size:
4703 209 181
BsrGI T'GTAC_A
Cuts at: 1041 1377 1545 1713 1797 2049 1041
Size: 336 168 168 84 252 4085
Fragments arranged by size:
4085 336 252 168 168 84
BssSI C'ACGA_G
Cuts at: 3364 3722 3364
Size: 358 4735
BstAPI GCAn_nnn'nTGC
Cuts at: 2470 2470
Size: 5093
BstBI TT'CG_AA
Cuts at: 110 3454 110
Size: 3344 1749
BstYI r'GATC_y
Cuts at: 1 242 2118 2585 2608 2943 3189 4201 4287
Size: 241 1876 467 23 335 246 1012 86
Cuts at: 4287 4299 4645 1
Size: 12 346 449
Fragments arranged by size:
1876 1012 467 449 346 335 246 241
86 23 12
BtgI C'CryG_G
Cuts at: 322 322
Size: 5093
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3043 3224 3387 3043
Size: 181 163 4749
Fragments arranged by size:
4749 181 163
BtsI GCAGTG_nn'
Cuts at: 753 921 1341 1509 1593 1761 2013 2336 753
Size: 168 420 168 84 168 252 323 3510
Fragments arranged by size:
3510 420 323 252 168 168 168 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 987 1022 1239 1274 1659 1694 987
Size: 35 217 35 385 35 4386
Fragments arranged by size:
4386 385 217 35 35 35
DraI TTT'AAA
Cuts at: 2092 2092
Size: 5093
DrdI GACnn_nn'nnGTC
Cuts at: 2799 3657 2799
Size: 858 4235
EaeI y'GGCC_r
Cuts at: 2678 2852 3243 3270 4615 2678
Size: 174 391 27 1345 3156
Fragments arranged by size:
3156 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2678 2678
Size: 5093
EarI CTCTTCn'nnn_
Cuts at: 415 937 1105 1273 1357 1525 1814 1845 1945
Size: 522 168 168 84 168 289 31 100
Cuts at: 1945 3116 3326 415
Size: 1171 210 2182
Fragments arranged by size:
2182 1171 522 289 210 168 168 168
100 84 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3301 3611 3757 4903 3301
Size: 310 146 1146 3491
Fragments arranged by size:
3491 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 439 837 998 1082 1586 1670 1754 1922
Size: 264 398 161 84 504 84 84 168
Cuts at: 1922 2918 3350 4097 4365 175
Size: 996 432 747 268 903
Fragments arranged by size:
996 903 747 504 432 398 268 264
168 161 84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 202 2232 202
Size: 2030 3063
EcoRI G'AATT_C
Cuts at: 884 2097 884
Size: 1213 3880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 5093
FspI TGC'GCA
Cuts at: 2874 2874
Size: 5093
HaeII r_GCGC'y
Cuts at: 2527 2775 3797 4776 2527
Size: 248 1022 979 2844
Fragments arranged by size:
2844 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 598 630 945 977 1281 1313 1533
Size: 32 286 32 315 32 304 32 220
Cuts at: 1533 1565 1617 1649 2935 2965 2967 2997 280
Size: 32 52 32 1286 30 2 30 2376
Fragments arranged by size:
2376 1286 315 304 286 220 52 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 558 2132 558
Size: 1574 3519
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 2113 2113
Size: 5093
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 284 3764 3948 218
Size: 66 3480 184 1363
Fragments arranged by size:
3480 1363 184 66
MscI TGG'CCA
Cuts at: 2854 2854
Size: 5093
MslI CAynn'nnrTG
Cuts at: 1245 1413 3082 3209 1245
Size: 168 1669 127 3129
Fragments arranged by size:
3129 1669 168 127
MspA1I CmG'CkG
Cuts at: 2204 2518 2878 3891 4136 2204
Size: 314 360 1013 245 3161
Fragments arranged by size:
3161 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3274 3274
Size: 5093
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 322 322
Size: 5093
NdeI CA'TA_TG
Cuts at: 994 1330 1666 994
Size: 336 336 4421
Fragments arranged by size:
4421 336 336
NgoMIV G'CCGG_C
Cuts at: 3272 3272
Size: 5093
NsiI A_TGCA'T
Cuts at: 453 453
Size: 5093
NspI r_CATG'y
Cuts at: 1119 1455 1502 2146 2646 3553 1119
Size: 336 47 644 500 907 2659
Fragments arranged by size:
2659 907 644 500 336 47
PciI A'CATG_T
Cuts at: 3549 3549
Size: 5093
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 5061
PspOMI G'GGCC_C
Cuts at: 201 201
Size: 5093
PstI C_TGCA'G
Cuts at: 392 654 2140 2825 392
Size: 262 1486 685 2660
Fragments arranged by size:
2660 1486 685 262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 2518 2878 2518
Size: 360 4733
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 5093
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5093
SalI G'TCGA_C
Cuts at: 2130 2130
Size: 5093
SapI GCTCTTCn'nnn_
Cuts at: 937 1105 1273 1945 3116 3326 937
Size: 168 168 672 1171 210 2704
Fragments arranged by size:
2704 1171 672 210 168 168
SbfI CC_TGCA'GG
Cuts at: 2140 2140
Size: 5093
ScaI AGT'ACT
Cuts at: 4610 4610
Size: 5093
SfcI C'TryA_G
Cuts at: 33 246 388 650 1164 2136 2821 3814 4005
Size: 213 142 262 514 972 685 993 191
Cuts at: 4005 33
Size: 1121
Fragments arranged by size:
1121 993 972 685 514 262 213 191
142
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 2115 2115
Size: 5093
SmlI C'TyrA_G
Cuts at: 2248 3163 3655 3917 2248
Size: 915 492 262 3424
Fragments arranged by size:
3424 915 492 262
SnaBI TAC'GTA
Cuts at: 2085 2085
Size: 5093
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 1119 1455 2146 2646 1119
Size: 336 691 500 3566
Fragments arranged by size:
3566 691 500 336
StyI C'CwwG_G
Cuts at: 322 1862 2227 2581 322
Size: 1540 365 354 2834
Fragments arranged by size:
2834 1540 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2676 2809 3268 3477 2676
Size: 133 459 209 4292
Fragments arranged by size:
4292 459 209 133
TatI w'GTAC_w
Cuts at: 670 829 1041 1377 1545 1713 1797 2049 4608
Size: 159 212 336 168 168 84 252 2559
Cuts at: 4608 670
Size: 1155
Fragments arranged by size:
2559 1155 336 252 212 168 168 159
84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 5061
Tth111I GACn'n_nGTC
Cuts at: 2890 2890
Size: 5093
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2588 2588
Size: 5093
XmaI C'CCGG_G
Cuts at: 2113 2113
Size: 5093
XmnI GAAnn'nnTTC
Cuts at: 1183 1267 1519 1603 1183
Size: 84 252 84 4673
Fragments arranged by size:
4673 252 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI