Restriction map for
pF1KSDA0813
(Circular) (Six-base) MAPSORT of: pF1KSDA0813.seq Check: 4587 from: 1 to: 7741
pF1KSDA0813 7741 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 250 955 994 2387 2950 3067 3217 3343 250
Size: 705 39 1393 563 117 150 126 4648
Fragments arranged by size:
4648 1393 705 563 150 126 117 39
AccI GT'mk_AC
Cuts at: 2467 4777 2467
Size: 2310 5431
Acc65I G'GTAC_C
Cuts at: 4755 4755
Size: 7741
AclI AA'CG_TT
Cuts at: 7581 7581
Size: 7741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 1846 2259 4654 5564 5996 6745 7013 1732
Size: 114 413 2395 910 432 749 268 2460
Fragments arranged by size:
2460 2395 910 749 432 413 268 114
AflII C'TTAA_G
Cuts at: 2520 2520
Size: 7741
AflIII A'CryG_T
Cuts at: 491 5091 6197 7174 491
Size: 4600 1106 977 1058
Fragments arranged by size:
4600 1106 1058 977
AgeI A'CCGG_T
Cuts at: 6129 6129
Size: 7741
AleI CACnn'nnGTG
Cuts at: 2334 4280 2334
Size: 1946 5795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 1931 4404 4414 295
Size: 1636 2473 10 3622
Fragments arranged by size:
3622 2473 1636 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 493 494 525 526 3073 3105 493
Size: 1 31 1 2547 32 5129
Fragments arranged by size:
5129 2547 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 813 1281 1335 1425 1894 4407 5044 6613 813
Size: 468 54 90 469 2513 637 1569 1941
Fragments arranged by size:
2513 1941 1569 637 469 468 90 54
ApaI G_GGCC'C
Cuts at: 859 2388 2813 3326 3414 4205 4377 859
Size: 1529 425 513 88 791 172 4223
Fragments arranged by size:
4223 1529 791 513 425 172 88
ApaLI G'TGCA_C
Cuts at: 981 6511 981
Size: 5530 2211
ApoI r'AATT_y
Cuts at: 606 4743 7094 7350 606
Size: 4137 2351 256 997
Fragments arranged by size:
4137 2351 997 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7741
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7741
AvaI C'yCGr_G
Cuts at: 139 852 1048 4347 4759 139
Size: 713 196 3299 412 3121
Fragments arranged by size:
3299 3121 713 412 196
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2063 2096 2063
Size: 33 7708
BamHI G'GATC_C
Cuts at: 350 3728 4764 350
Size: 3378 1036 3327
Fragments arranged by size:
3378 3327 1036
BanI G'GyrC_C
Cuts at: 206 214 1513 1535 2716 2938 3055 3310 3972
Size: 8 1299 22 1181 222 117 255 662
Cuts at: 3972 4045 4131 4432 4755 5169 5417 5452 206
Size: 73 86 301 323 414 248 35 2495
Fragments arranged by size:
2495 1299 1181 662 414 323 301 255
248 222 117 86 73 35 22 8
BanII G_rGCy'C
Cuts at: 179 377 839 859 2388 2813 3326 3414 3514
Size: 198 462 20 1529 425 513 88 100
Cuts at: 3514 3925 4205 4377 4388 4597 4753 5783 179
Size: 411 280 172 11 209 156 1030 2137
Fragments arranged by size:
2137 1529 1030 513 462 425 411 280
209 198 172 156 100 88 20 11
BbeI G_GCGC'C
Cuts at: 210 1539 3976 5173 5421 210
Size: 1329 2437 1197 248 2530
Fragments arranged by size:
2530 2437 1329 1197 248
BbsI GAAGACnn'nnnn_
Cuts at: 2776 2776
Size: 7741
BbvCI CC'TCA_GC
Cuts at: 814 2569 3455 814
Size: 1755 886 5100
Fragments arranged by size:
5100 1755 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 981 1015 3742 3776 981
Size: 34 2727 34 4946
Fragments arranged by size:
4946 2727 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2091 5637 6400 7741 2091
Size: 3546 763 1341 2091
Fragments arranged by size:
3546 2091 1341 763
BclI T'GATC_A
Cuts at: 5259 5259
Size: 7741
BglI GCCn_nnn'nGGC
Cuts at: 168 562 872 1036 2987 3590 4172 4412 168
Size: 394 310 164 1951 603 582 240 3497
Fragments arranged by size:
3497 1951 603 582 394 310 240 164
BglII A'GATC_T
Cuts at: 5254 7293 5254
Size: 2039 5702
BlpI GC'TnA_GC
Cuts at: 1587 4851 1587
Size: 3264 4477
Bme1580I G_kGCm'C
Cuts at: 229 859 944 985 1396 1518 2329 2388 2813
Size: 630 85 41 411 122 811 59 425
Cuts at: 2813 2941 3058 3220 3313 3326 3414 4205 4304
Size: 128 117 162 93 13 88 791 99
Cuts at: 4304 4356 4377 4446 4603 4719 5364 5457 6515
Size: 52 21 69 157 116 645 93 1058
Cuts at: 6515 229
Size: 1455
Fragments arranged by size:
1455 1058 811 791 645 630 425 411
162 157 128 122 117 116 99 93
93 88 85 69 59 52 41 21
13
BmgBI CAC'GTC
Cuts at: 1656 2259 2688 1656
Size: 603 429 6709
Fragments arranged by size:
6709 603 429
BmrI ACTGGGnnnn_n'
Cuts at: 840 1737 1808 3294 3408 4951 5032 5367 7513
Size: 897 71 1486 114 1543 81 335 2146
Cuts at: 7513 840
Size: 1068
Fragments arranged by size:
2146 1543 1486 1068 897 335 114 81
71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6106 6106
Size: 7741
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 694 1093 1124 1689 1810 1948 2089 478
Size: 216 399 31 565 121 138 141 6130
Fragments arranged by size:
6130 565 399 216 141 138 121 31
Bpu10I CC'TnA_GC
Cuts at: 814 915 1144 1730 2497 2569 3455 814
Size: 101 229 586 767 72 886 5100
Fragments arranged by size:
5100 886 767 586 229 101 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 564 1450 1989 2980 4915 5794 6288 6586
Size: 203 886 539 991 1935 879 494 298
Cuts at: 6586 361
Size: 1516
Fragments arranged by size:
1935 1516 991 886 879 539 494 298
203
BsaI GGTCTCn'nnnn_
Cuts at: 1045 3711 7445 1045
Size: 2666 3734 1341
Fragments arranged by size:
3734 2666 1341
BsaAI yAC'GTr
Cuts at: 3095 4731 5092 3095
Size: 1636 361 5744
Fragments arranged by size:
5744 1636 361
BsaBI GATnn'nnATC
Cuts at: 6 1783 5253 7292 6
Size: 1777 3470 2039 455
Fragments arranged by size:
3470 2039 1777 455
BsaHI Gr'CG_yC
Cuts at: 207 1178 1536 3179 3973 5170 5418 6122 7618
Size: 971 358 1643 794 1197 248 704 1496
Cuts at: 7618 207
Size: 330
Fragments arranged by size:
1643 1496 1197 971 794 704 358 330
248
BsaWI w'CCGG_w
Cuts at: 247 498 1580 1937 3270 4928 5449 6129 6403
Size: 251 1082 357 1333 1658 521 680 274
Cuts at: 6403 6550 6961 247
Size: 147 411 1027
Fragments arranged by size:
1658 1333 1082 1027 680 521 411 357
274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2111 2141 2677 2707 3424 3454 2111
Size: 30 536 30 717 30 6398
Fragments arranged by size:
6398 717 536 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2038 2311 2827 4699 2038
Size: 273 516 1872 5080
Fragments arranged by size:
5080 1872 516 273
BseYI C'CCAG_C
Cuts at: 858 2762 2806 2983 3817 4226 5164 5922 6501
Size: 1904 44 177 834 409 938 758 579
Cuts at: 6501 6997 858
Size: 496 1602
Fragments arranged by size:
1904 1602 938 834 758 579 496 409
177 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 228 472 745 933 1411 1891 4222 4680
Size: 6 244 273 188 478 480 2331 458
Cuts at: 4680 222
Size: 3283
Fragments arranged by size:
3283 2331 480 478 458 273 244 188
6
BsiEI CG_ry'CG
Cuts at: 104 172 5281 5327 6537 104
Size: 68 5109 46 1210 1308
Fragments arranged by size:
5109 1308 1210 68 46
BsiHKAI G_wGCw'C
Cuts at: 179 663 853 985 1588 1705 3100 3268 4753
Size: 484 190 132 603 117 1395 168 1485
Cuts at: 4753 5531 5721 6515 179
Size: 778 190 794 1405
Fragments arranged by size:
1485 1405 1395 794 778 603 484 190
190 168 132 117
BsmI GAATG_Cn'
Cuts at: 1296 2399 2869 1296
Size: 1103 470 6168
Fragments arranged by size:
6168 1103 470
BsmBI CGTCTCn'nnnn_
Cuts at: 1498 3835 1498
Size: 2337 5404
Bsp1286I G_dGCh'C
Cuts at: 179 229 377 663 839 853 859 944 985
Size: 50 148 286 176 14 6 85 41
Cuts at: 985 1396 1518 1588 1705 2329 2388 2813 2941
Size: 411 122 70 117 624 59 425 128
Cuts at: 2941 3058 3100 3220 3268 3313 3326 3414 3514
Size: 117 42 120 48 45 13 88 100
Cuts at: 3514 3925 4205 4304 4356 4377 4388 4446 4597
Size: 411 280 99 52 21 11 58 151
Cuts at: 4597 4603 4719 4753 5364 5457 5531 5721 5783
Size: 6 116 34 611 93 74 190 62
Cuts at: 5783 6515 179
Size: 732 1405
Fragments arranged by size:
1405 732 624 611 425 411 411 286
280 190 176 151 148 128 122 120
117 117 116 100 99 93 88 85
74 70 62 59 58 52 50 48
45 42 41 34 21 14 13 11
6 6
BspEI T'CCGG_A
Cuts at: 1937 3270 6961 1937
Size: 1333 3691 2717
Fragments arranged by size:
3691 2717 1333
BspHI T'CATG_A
Cuts at: 6917 6917
Size: 7741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 250 955 994 1052 1343 2387 2489 2950 3067
Size: 705 39 58 291 1044 102 461 117
Cuts at: 3067 3217 3343 3662 3677 3693 4789 5003 5305
Size: 150 126 319 15 16 1096 214 302
Cuts at: 5305 5686 250
Size: 381 2305
Fragments arranged by size:
2305 1096 1044 705 461 381 319 302
291 214 150 126 117 102 58 39
16 15
BsrBI CCG'CTC
Cuts at: 50 462 1196 1943 2621 6031 6085 7571 7736
Size: 412 734 747 678 3410 54 1486 165
Cuts at: 7736 50
Size: 55
Fragments arranged by size:
3410 1486 747 734 678 412 165 55
54
BsrDI GCAATG_nn'
Cuts at: 999 2655 2792 3758 4300 5651 999
Size: 1656 137 966 542 1351 3089
Fragments arranged by size:
3089 1656 1351 966 542 137
BsrFI r'CCGG_y
Cuts at: 162 3109 4048 5737 5918 6129 162
Size: 2947 939 1689 181 211 1774
Fragments arranged by size:
2947 1774 1689 939 211 181
BsrGI T'GTAC_A
Cuts at: 2966 3737 4076 2966
Size: 771 339 6631
Fragments arranged by size:
6631 771 339
BssSI C'ACGA_G
Cuts at: 2488 3292 4596 6010 6370 2488
Size: 804 1304 1414 360 3859
Fragments arranged by size:
3859 1414 1304 804 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2003 4299 4407 5116 2003
Size: 2296 108 709 4628
Fragments arranged by size:
4628 2296 709 108
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7741
BstXI CCAn_nnnn'nTGG
Cuts at: 1695 1926 3552 3933 1695
Size: 231 1626 381 5503
Fragments arranged by size:
5503 1626 381 231
BstYI r'GATC_y
Cuts at: 1 350 1373 1724 3273 3482 3728 4034 4764
Size: 349 1023 351 1549 209 246 306 730
Cuts at: 4764 5231 5254 5589 5835 6849 6935 6947 7293
Size: 467 23 335 246 1014 86 12 346
Cuts at: 7293 1
Size: 449
Fragments arranged by size:
1549 1023 1014 730 467 449 351 349
346 335 306 246 246 209 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 4428 4428
Size: 7741
BtgI C'CryG_G
Cuts at: 125 254 267 1245 1748 2354 2643 3682 3710
Size: 129 13 978 503 606 289 1039 28
Cuts at: 3710 3932 3998 4056 4269 4287 125
Size: 222 66 58 213 18 3579
Fragments arranged by size:
3579 1039 978 606 503 289 222 213
129 66 58 28 18 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2229 4679 5689 5870 6033 2229
Size: 2450 1010 181 163 3937
Fragments arranged by size:
3937 2450 1010 181 163
BtsI GCAGTG_nn'
Cuts at: 814 1025 1348 2593 3372 4212 4408 4416 4982
Size: 211 323 1245 779 840 196 8 566
Cuts at: 4982 814
Size: 3573
Fragments arranged by size:
3573 1245 840 779 566 323 211 196
8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 760 795 3096 3131 760
Size: 35 2301 35 5370
Fragments arranged by size:
5370 2301 35 35
DraI TTT'AAA
Cuts at: 1809 4738 1809
Size: 2929 4812
DraIII CAC_nnn'GTG
Cuts at: 848 4450 4452 4706 848
Size: 3602 2 254 3883
Fragments arranged by size:
3883 3602 254 2
DrdI GACnn_nn'nnGTC
Cuts at: 2356 2697 5445 6305 2356
Size: 341 2748 860 3792
Fragments arranged by size:
3792 2748 860 341
EaeI y'GGCC_r
Cuts at: 169 823 974 1037 2132 2708 3679 4272 4413
Size: 654 151 63 1095 576 971 593 141
Cuts at: 4413 5324 5498 5889 5916 7263 169
Size: 911 174 391 27 1347 647
Fragments arranged by size:
1347 1095 971 911 654 647 593 576
391 174 151 141 63 27
EagI C'GGCC_G
Cuts at: 169 5324 169
Size: 5155 2586
EarI CTCTTCn'nnn_
Cuts at: 844 892 5762 5972 844
Size: 48 4870 210 2613
Fragments arranged by size:
4870 2613 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 745 4012 5947 6259 6405 7551 745
Size: 3267 1935 312 146 1146 935
Fragments arranged by size:
3267 1935 1146 935 312 146
EcoICRI GAG'CTC
Cuts at: 177 4751 177
Size: 4574 3167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 694 1093 1124 1689 1732 1810 1846 1948
Size: 216 399 31 565 43 78 36 102
Cuts at: 1948 2089 2259 4654 5564 5996 6745 7013 478
Size: 141 170 2395 910 432 749 268 1206
Fragments arranged by size:
2395 1206 910 749 565 432 399 268
216 170 141 102 78 43 36 31
EcoNI CCTnn'n_nnAGG
Cuts at: 1387 2392 2668 1387
Size: 1005 276 6460
Fragments arranged by size:
6460 1005 276
EcoO109I rG'GnC_Cy
Cuts at: 149 650 1188 1332 1874 1973 2385 2810 2872
Size: 501 538 144 542 99 412 425 62
Cuts at: 2872 3410 4201 4373 4374 4509 4623 4670 4878
Size: 538 791 172 1 135 114 47 208
Cuts at: 4878 149
Size: 3012
Fragments arranged by size:
3012 791 542 538 538 501 425 412
208 172 144 135 114 99 62 47
1
EcoRI G'AATT_C
Cuts at: 4743 4743
Size: 7741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 4668 5758 381
Size: 4287 1090 2364
Fragments arranged by size:
4287 2364 1090
FspI TGC'GCA
Cuts at: 1489 2035 4725 5520 1489
Size: 546 2690 795 3710
Fragments arranged by size:
3710 2690 795 546
HaeII r_GCGC'y
Cuts at: 133 210 321 1539 3976 5173 5421 6445 7424
Size: 77 111 1218 2437 1197 248 1024 979
Cuts at: 7424 133
Size: 450
Fragments arranged by size:
2437 1218 1197 1024 979 450 248 111
77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1137 1169 2677 2709 3250 3282 3885 3917 3994
Size: 32 1508 32 541 32 603 32 77
Cuts at: 3994 4026 4330 4362 4477 4509 4657 4689 5581
Size: 32 304 32 115 32 148 32 892
Cuts at: 5581 5611 5613 5643 1137
Size: 30 2 30 3235
Fragments arranged by size:
3235 1508 892 603 541 304 148 115
77 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3289 3432 3630 4008 4778 3289
Size: 143 198 378 770 6252
Fragments arranged by size:
6252 770 378 198 143
HindIII A'AGCT_T
Cuts at: 578 578
Size: 7741
KasI G'GCGC_C
Cuts at: 206 1535 3972 5169 5417 206
Size: 1329 2437 1197 248 2530
Fragments arranged by size:
2530 2437 1329 1197 248
KpnI G_GTAC'C
Cuts at: 4759 4759
Size: 7741
MluI A'CGCG_T
Cuts at: 7174 7174
Size: 7741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 2902 6412 6596 791
Size: 2111 3510 184 1936
Fragments arranged by size:
3510 2111 1936 184
MscI TGG'CCA
Cuts at: 2134 2710 3681 4274 4415 5500 2134
Size: 576 971 593 141 1085 4375
Fragments arranged by size:
4375 1085 971 593 576 141
MslI CAynn'nnrTG
Cuts at: 359 715 1801 2334 2542 2648 3164 3788 3931
Size: 356 1086 533 208 106 516 624 143
Cuts at: 3931 4280 5728 5855 359
Size: 349 1448 127 2245
Fragments arranged by size:
2245 1448 1086 624 533 516 356 349
208 143 127 106
MspA1I CmG'CkG
Cuts at: 384 399 486 615 635 822 911 1520 1632
Size: 15 87 129 20 187 89 609 112
Cuts at: 1632 1679 1926 2003 2160 2463 2553 2750 2762
Size: 47 247 77 157 303 90 197 12
Cuts at: 2762 2987 3026 3252 3398 4015 4255 4325 4850
Size: 225 39 226 146 617 240 70 525
Cuts at: 4850 5164 5524 6539 6784 384
Size: 314 360 1015 245 1341
Fragments arranged by size:
1341 1015 617 609 525 360 314 303
247 245 240 226 225 197 187 157
146 129 112 90 89 87 77 70
47 39 20 15 12
NaeI GCC'GGC
Cuts at: 5920 5920
Size: 7741
NarI GG'CG_CC
Cuts at: 207 1536 3973 5170 5418 207
Size: 1329 2437 1197 248 2530
Fragments arranged by size:
2530 2437 1329 1197 248
NcoI C'CATG_G
Cuts at: 125 3932 4269 4287 125
Size: 3807 337 18 3579
Fragments arranged by size:
3807 3579 337 18
NdeI CA'TA_TG
Cuts at: 734 734
Size: 7741
NgoMIV G'CCGG_C
Cuts at: 5918 5918
Size: 7741
NruI TCG'CGA
Cuts at: 6102 6102
Size: 7741
NspI r_CATG'y
Cuts at: 495 1950 3342 4792 5292 6201 495
Size: 1455 1392 1450 500 909 2035
Fragments arranged by size:
2035 1455 1450 1392 909 500
PasI CC'CwG_GG
Cuts at: 4198 4198
Size: 7741
PciI A'CATG_T
Cuts at: 491 6197 491
Size: 5706 2035
PflMI CCAn_nnn'nTGG
Cuts at: 2820 3130 3306 3420 2820
Size: 310 176 114 7141
Fragments arranged by size:
7141 310 176 114
PfoI T'CCnGG_A
Cuts at: 1101 1328 1787 2454 4517 1101
Size: 227 459 667 2063 4325
Fragments arranged by size:
4325 2063 667 459 227
PmeI GTTT'AAAC
Cuts at: 4738 4738
Size: 7741
PmlI CAC'GTG
Cuts at: 3095 3095
Size: 7741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6880 6912 6880
Size: 32 7709
PpuMI rG'GwC_Cy
Cuts at: 149 1332 1973 2872 4509 4670 149
Size: 1183 641 899 1637 161 3220
Fragments arranged by size:
3220 1637 1183 899 641 161
PshAI GACnn'nnGTC
Cuts at: 2463 2463
Size: 7741
PspOMI G'GGCC_C
Cuts at: 855 2384 2809 3322 3410 4201 4373 855
Size: 1529 425 513 88 791 172 4223
Fragments arranged by size:
4223 1529 791 513 425 172 88
PstI C_TGCA'G
Cuts at: 284 398 991 1340 2255 2486 2588 3227 3367
Size: 114 593 349 915 231 102 639 140
Cuts at: 3367 3388 3659 4097 4393 4403 4786 5471 284
Size: 21 271 438 296 10 383 685 2554
Fragments arranged by size:
2554 915 685 639 593 438 383 349
296 271 231 140 114 102 21 10
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7741
PvuII CAG'CTG
Cuts at: 384 399 486 615 635 911 1632 1926 2003
Size: 15 87 129 20 276 721 294 77
Cuts at: 2003 2160 2463 2553 2762 3026 3252 4015 4325
Size: 157 303 90 209 264 226 763 310
Cuts at: 4325 5164 5524 384
Size: 839 360 2601
Fragments arranged by size:
2601 839 763 721 360 310 303 294
276 264 226 209 157 129 90 87
77 20 15
RsrII CG'GwC_CG
Cuts at: 165 5934 165
Size: 5769 1972
SacI G_AGCT'C
Cuts at: 179 4753 179
Size: 4574 3167
SalI G'TCGA_C
Cuts at: 4776 4776
Size: 7741
SanDI GG'GwC_CC
Cuts at: 4509 4509
Size: 7741
SapI GCTCTTCn'nnn_
Cuts at: 844 5762 5972 844
Size: 4918 210 2613
Fragments arranged by size:
4918 2613 210
SbfI CC_TGCA'GG
Cuts at: 2255 4786 2255
Size: 2531 5210
ScaI AGT'ACT
Cuts at: 1603 7258 1603
Size: 5655 2086
SexAI A'CCwGG_T
Cuts at: 4529 4529
Size: 7741
SfcI C'TryA_G
Cuts at: 33 280 394 987 1119 1273 1336 2251 2482
Size: 247 114 593 132 154 63 915 231
Cuts at: 2482 2584 3223 3363 3384 3462 3655 3831 4093
Size: 102 639 140 21 78 193 176 262
Cuts at: 4093 4320 4389 4399 4782 5467 6462 6653 33
Size: 227 69 10 383 685 995 191 1121
Fragments arranged by size:
1121 995 915 685 639 593 383 262
247 231 227 193 191 176 154 140
132 114 102 78 69 63 21 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 168 168
Size: 7741
SfoI GGC'GCC
Cuts at: 208 1537 3974 5171 5419 208
Size: 1329 2437 1197 248 2530
Fragments arranged by size:
2530 2437 1329 1197 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7741
SmaI CCC'GGG
Cuts at: 141 4761 141
Size: 4620 3121
SmlI C'TyrA_G
Cuts at: 376 543 1465 1968 2520 2959 4894 5809 6303
Size: 167 922 503 552 439 1935 915 494
Cuts at: 6303 6565 376
Size: 262 1552
Fragments arranged by size:
1935 1552 922 915 552 503 494 439
262 167
SnaBI TAC'GTA
Cuts at: 4731 4731
Size: 7741
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7741
SphI G_CATG'C
Cuts at: 3342 4792 5292 3342
Size: 1450 500 5791
Fragments arranged by size:
5791 1450 500
StuI AGG'CCT
Cuts at: 1022 1262 3241 3570 4397 4426 1022
Size: 240 1979 329 827 29 4337
Fragments arranged by size:
4337 1979 827 329 240 29
StyI C'CwwG_G
Cuts at: 125 1388 2393 2868 3932 4269 4287 4436 4873
Size: 1263 1005 475 1064 337 18 149 437
Cuts at: 4873 5227 125
Size: 354 2639
Fragments arranged by size:
2639 1263 1064 1005 475 437 354 337
149 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 859 2754 4218 4569 5322 5455 5914 6125
Size: 657 1895 1464 351 753 133 459 211
Cuts at: 6125 202
Size: 1818
Fragments arranged by size:
1895 1818 1464 753 657 459 351 211
133
TatI w'GTAC_w
Cuts at: 1601 2966 3737 4076 7256 1601
Size: 1365 771 339 3180 2086
Fragments arranged by size:
3180 2086 1365 771 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 2111 2143 2436 2468 7258 7290 242
Size: 32 1837 32 293 32 4790 32 693
Fragments arranged by size:
4790 1837 693 293 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1657 4513 5536 1657
Size: 2856 1023 3862
Fragments arranged by size:
3862 2856 1023
XbaI T'CTAG_A
Cuts at: 4770 4770
Size: 7741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4219 4336 5234 4219
Size: 117 898 6726
Fragments arranged by size:
6726 898 117
XmaI C'CCGG_G
Cuts at: 139 4759 139
Size: 4620 3121
XmnI GAAnn'nnTTC
Cuts at: 1444 2440 1444
Size: 996 6745
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BfrBI BmtI BsiWI
BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NheI NotI NsiI PacI PsiI PspXI PsrI
SacII SgrAI SrfI SspI SwaI XhoI ZraI