Restriction map for
pF1KSDA0814
(Circular) (Six-base) MAPSORT of: pF1KSDA0814.seq Check: 1075 from: 1 to: 7706
pF1KSDA0814 7706 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3007 3040 3163 3430 3559 3669 3007
Size: 33 123 267 129 110 7044
Fragments arranged by size:
7044 267 129 123 110 33
AccI GT'mk_AC
Cuts at: 4744 4744
Size: 7706
Acc65I G'GTAC_C
Cuts at: 4722 4722
Size: 7706
AclI AA'CG_TT
Cuts at: 7546 7546
Size: 7706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1144 2493 2663 3420 5531 5963 6710 6978
Size: 675 1349 170 757 2111 432 747 268
Cuts at: 6978 469
Size: 1197
Fragments arranged by size:
2111 1349 1197 757 747 675 432 268
170
AfeI AGC'GCT
Cuts at: 662 662
Size: 7706
AflIII A'CryG_T
Cuts at: 908 1316 2516 3179 4292 5058 6162 7139 908
Size: 408 1200 663 1113 766 1104 977 1475
Fragments arranged by size:
1475 1200 1113 1104 977 766 663 408
AgeI A'CCGG_T
Cuts at: 6094 6094
Size: 7706
AhdI GACnn_n'nnGTC
Cuts at: 4483 4483
Size: 7706
AleI CACnn'nnGTG
Cuts at: 598 1669 3041 3157 598
Size: 1071 1372 116 5147
Fragments arranged by size:
5147 1372 1071 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2506 3550 2506
Size: 1044 6662
AlwNI CAG_nnn'CTG
Cuts at: 447 1185 1329 1692 1881 3873 4101 5011 6578
Size: 738 144 363 189 1992 228 910 1567
Cuts at: 6578 447
Size: 1575
Fragments arranged by size:
1992 1575 1567 910 738 363 228 189
144
ApaI G_GGCC'C
Cuts at: 1512 3387 3522 4577 1512
Size: 1875 135 1055 4641
Fragments arranged by size:
4641 1875 1055 135
ApaLI G'TGCA_C
Cuts at: 1912 2310 4626 6476 1912
Size: 398 2316 1850 3142
Fragments arranged by size:
3142 2316 1850 398
ApoI r'AATT_y
Cuts at: 4710 7059 7315 4710
Size: 2349 256 5101
Fragments arranged by size:
5101 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
AvaI C'yCGr_G
Cuts at: 133 293 570 1642 4229 4726 133
Size: 160 277 1072 2587 497 3113
Fragments arranged by size:
3113 2587 1072 497 277 160
AvrII C'CTAG_G
Cuts at: 2188 2188
Size: 7706
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3168 3201 3168
Size: 33 7673
BamHI G'GATC_C
Cuts at: 566 4731 566
Size: 4165 3541
BanI G'GyrC_C
Cuts at: 151 433 2192 3028 4542 4722 5136 5384 5419
Size: 282 1759 836 1514 180 414 248 35
Cuts at: 5419 151
Size: 2438
Fragments arranged by size:
2438 1759 1514 836 414 282 248 180
35
BanII G_rGCy'C
Cuts at: 377 935 1391 1512 2293 2354 2792 3387 3522
Size: 558 456 121 781 61 438 595 135
Cuts at: 3522 3548 4415 4577 4720 5750 377
Size: 26 867 162 143 1030 2333
Fragments arranged by size:
2333 1030 867 781 595 558 456 438
162 143 135 121 61 26
BbeI G_GCGC'C
Cuts at: 155 437 5140 5388 155
Size: 282 4703 248 2473
Fragments arranged by size:
4703 2473 282 248
BbsI GAAGACnn'nnnn_
Cuts at: 410 2710 4611 410
Size: 2300 1901 3505
Fragments arranged by size:
3505 2300 1901
BbvCI CC'TCA_GC
Cuts at: 1960 2557 1960
Size: 597 7109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 975 1009 3130 3164 975
Size: 34 2121 34 5517
Fragments arranged by size:
5517 2121 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5604 6365 7706 5604
Size: 761 1341 5604
Fragments arranged by size:
5604 1341 761
BclI T'GATC_A
Cuts at: 5226 5226
Size: 7706
BfrBI ATG'CAT
Cuts at: 4040 4519 4040
Size: 479 7227
BglI GCCn_nnn'nGGC
Cuts at: 172 1940 3083 3431 3566 4196 4433 172
Size: 1768 1143 348 135 630 237 3445
Fragments arranged by size:
3445 1768 1143 630 348 237 135
BglII A'GATC_T
Cuts at: 5221 7258 5221
Size: 2037 5669
BlpI GC'TnA_GC
Cuts at: 314 2287 4362 4818 314
Size: 1973 2075 456 3202
Fragments arranged by size:
3202 2075 1973 456
Bme1580I G_kGCm'C
Cuts at: 916 1512 1642 1666 1916 2314 2429 2990 3387
Size: 596 130 24 250 398 115 561 397
Cuts at: 3387 3522 4009 4547 4577 4630 5331 5424 6480
Size: 135 487 538 30 53 701 93 1056
Cuts at: 6480 916
Size: 2142
Fragments arranged by size:
2142 1056 701 596 561 538 487 398
397 250 135 130 115 93 53 30
24
BmrI ACTGGGnnnn_n'
Cuts at: 1104 3381 3516 4289 4572 4918 4999 5334 7478
Size: 2277 135 773 283 346 81 335 2144
Cuts at: 7478 1104
Size: 1332
Fragments arranged by size:
2277 2144 1332 773 346 335 283 135
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 227 3809 6073 227
Size: 3582 2264 1860
Fragments arranged by size:
3582 2264 1860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1151 1465 1786 1924 2874 3763 3904 197
Size: 954 314 321 138 950 889 141 3999
Fragments arranged by size:
3999 954 950 889 321 314 141 138
Bpu10I CC'TnA_GC
Cuts at: 1581 1960 2470 2542 2557 2786 3245 1581
Size: 379 510 72 15 229 459 6042
Fragments arranged by size:
6042 510 459 379 229 72 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 2197 4882 5761 6253 6551 361
Size: 1836 2685 879 492 298 1516
Fragments arranged by size:
2685 1836 1516 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1900 7410 1900
Size: 5510 2196
BsaAI yAC'GTr
Cuts at: 909 980 3180 3630 3762 4698 5059 909
Size: 71 2200 450 132 936 361 3556
Fragments arranged by size:
3556 2200 936 450 361 132 71
BsaBI GATnn'nnATC
Cuts at: 6 2224 5220 7257 6
Size: 2218 2996 2037 455
Fragments arranged by size:
2996 2218 2037 455
BsaHI Gr'CG_yC
Cuts at: 152 434 5137 5385 6087 7583 152
Size: 282 4703 248 702 1496 275
Fragments arranged by size:
4703 1496 702 282 275 248
BsaWI w'CCGG_w
Cuts at: 4895 5416 6094 6368 6515 6926 4895
Size: 521 678 274 147 411 5675
Fragments arranged by size:
5675 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 895 925 943 973 1259 1289 1333 1363 895
Size: 30 18 30 286 30 44 30 7238
Fragments arranged by size:
7238 286 44 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1267 1270 1977 2284 4629 1267
Size: 3 707 307 2345 4344
Fragments arranged by size:
4344 2345 707 307 3
BseYI C'CCAG_C
Cuts at: 273 370 915 2685 5131 5889 6466 6962 273
Size: 97 545 1770 2446 758 577 496 1017
Fragments arranged by size:
2446 1770 1017 758 577 545 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 960 1002 1894 2964 3556 4113 913
Size: 47 42 892 1070 592 557 4506
Fragments arranged by size:
4506 1070 892 592 557 47 42
BsiEI CG_ry'CG
Cuts at: 104 5248 5294 6502 104
Size: 5144 46 1208 1308
Fragments arranged by size:
5144 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 520 1916 2314 4451 4630 4720 5498 5688 6480
Size: 1396 398 2137 179 90 778 190 792
Cuts at: 6480 520
Size: 1746
Fragments arranged by size:
2137 1746 1396 792 778 398 190 179
90
Bsp1286I G_dGCh'C
Cuts at: 377 520 916 935 1391 1512 1642 1666 1916
Size: 143 396 19 456 121 130 24 250
Cuts at: 1916 2293 2314 2354 2429 2792 2990 3387 3522
Size: 377 21 40 75 363 198 397 135
Cuts at: 3522 3548 4009 4415 4451 4547 4577 4630 4720
Size: 26 461 406 36 96 30 53 90
Cuts at: 4720 5331 5424 5498 5688 5750 6480 377
Size: 611 93 74 190 62 730 1603
Fragments arranged by size:
1603 730 611 461 456 406 397 396
377 363 250 198 190 143 135 130
121 96 93 90 75 74 62 53
40 36 30 26 24 21 19
BspEI T'CCGG_A
Cuts at: 6926 6926
Size: 7706
BspHI T'CATG_A
Cuts at: 6882 6882
Size: 7706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 250 617 1337 1595 1742 1808 3007 3040 3163
Size: 367 720 258 147 66 1199 33 123
Cuts at: 3163 3430 3559 3669 4756 4970 5272 5653 250
Size: 267 129 110 1087 214 302 381 2303
Fragments arranged by size:
2303 1199 1087 720 381 367 302 267
258 214 147 129 123 110 66 33
BsrBI CCG'CTC
Cuts at: 50 4157 5998 6052 7536 7701 50
Size: 4107 1841 54 1484 165 55
Fragments arranged by size:
4107 1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 951 2628 2883 5618 951
Size: 1677 255 2735 3039
Fragments arranged by size:
3039 2735 1677 255
BsrFI r'CCGG_y
Cuts at: 2002 2552 3574 3982 4013 4204 5704 5885 6094
Size: 550 1022 408 31 191 1500 181 209
Cuts at: 6094 2002
Size: 3614
Fragments arranged by size:
3614 1500 1022 550 408 209 191 181
31
BsrGI T'GTAC_A
Cuts at: 3806 3806
Size: 7706
BssHII G'CGCG_C
Cuts at: 162 162
Size: 7706
BssSI C'ACGA_G
Cuts at: 1633 5977 6335 1633
Size: 4344 358 3004
Fragments arranged by size:
4344 3004 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1619 2882 2978 4127 4136 4525 5083 1619
Size: 1263 96 1149 9 389 558 4242
Fragments arranged by size:
4242 1263 1149 558 389 96 9
BstBI TT'CG_AA
Cuts at: 110 6067 110
Size: 5957 1749
BstEII G'GTnAC_C
Cuts at: 717 717
Size: 7706
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3159 3058
Size: 101 7605
BstYI r'GATC_y
Cuts at: 1 557 566 677 743 4731 5198 5221 5556
Size: 556 9 111 66 3988 467 23 335
Cuts at: 5556 5802 6814 6900 6912 7258 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3988 1012 556 467 449 346 335 246
111 86 66 23 12 9
BtgI C'CryG_G
Cuts at: 125 267 585 1929 2616 2792 3378 3467 4032
Size: 142 318 1344 687 176 586 89 565
Cuts at: 4032 4160 4260 4380 125
Size: 128 100 120 3451
Fragments arranged by size:
3451 1344 687 586 565 318 176 142
128 120 100 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1184 2349 2751 5656 5837 6000 1184
Size: 1165 402 2905 181 163 2890
Fragments arranged by size:
2905 2890 1165 402 181 163
BtsI GCAGTG_nn'
Cuts at: 2314 3941 4393 4449 4949 2314
Size: 1627 452 56 500 5071
Fragments arranged by size:
5071 1627 500 452 56
DraI TTT'AAA
Cuts at: 4705 4705
Size: 7706
DraIII CAC_nnn'GTG
Cuts at: 236 3240 3846 4136 236
Size: 3004 606 290 3806
Fragments arranged by size:
3806 3004 606 290
DrdI GACnn_nn'nnGTC
Cuts at: 412 5412 6270 412
Size: 5000 858 1848
Fragments arranged by size:
5000 1848 858
EaeI y'GGCC_r
Cuts at: 834 1469 2237 2867 3683 4245 5291 5465 5856
Size: 635 768 630 816 562 1046 174 391
Cuts at: 5856 5883 7228 834
Size: 27 1345 1312
Fragments arranged by size:
1345 1312 1046 816 768 635 630 562
391 174 27
EagI C'GGCC_G
Cuts at: 5291 5291
Size: 7706
EarI CTCTTCn'nnn_
Cuts at: 1396 2158 4646 5729 5939 1396
Size: 762 2488 1083 210 3163
Fragments arranged by size:
3163 2488 1083 762 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 901 2184 2430 3182 3633 4489 4623 5914 6224
Size: 1283 246 752 451 856 134 1291 310
Cuts at: 6224 6370 7516 901
Size: 146 1146 1091
Fragments arranged by size:
1291 1283 1146 1091 856 752 451 310
246 146 134
EcoICRI GAG'CTC
Cuts at: 4718 4718
Size: 7706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 469 1144 1151 1465 1786 1924 2493 2663
Size: 272 675 7 314 321 138 569 170
Cuts at: 2663 2874 3420 3763 3904 5531 5963 6710 6978
Size: 211 546 343 141 1627 432 747 268
Cuts at: 6978 197
Size: 925
Fragments arranged by size:
1627 925 747 675 569 546 432 343
321 314 272 268 211 170 141 138
7
EcoO109I rG'GnC_Cy
Cuts at: 444 1182 1326 1508 2066 2925 3383 3518 4083
Size: 738 144 182 558 859 458 135 565
Cuts at: 4083 4233 4283 4574 4845 444
Size: 150 50 291 271 3305
Fragments arranged by size:
3305 859 738 565 558 458 291 271
182 150 144 135 50
EcoRI G'AATT_C
Cuts at: 4710 4710
Size: 7706
EcoRV GAT'ATC
Cuts at: 1154 1154
Size: 7706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 1407 5725 381
Size: 1026 4318 2362
Fragments arranged by size:
4318 2362 1026
FspI TGC'GCA
Cuts at: 4054 5487 4054
Size: 1433 6273
FspAI rTGC'GCAy
Cuts at: 4054 4054
Size: 7706
HaeII r_GCGC'y
Cuts at: 155 178 190 321 437 664 2686 5140 5388
Size: 23 12 131 116 227 2022 2454 248
Cuts at: 5388 6410 7389 155
Size: 1022 979 472
Fragments arranged by size:
2454 2022 1022 979 472 248 227 131
116 23 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 735 767 922 954 2650 2682 4469 4481 4501
Size: 32 155 32 1696 32 1787 12 20
Cuts at: 4501 4513 4624 4656 5548 5578 5580 5610 735
Size: 12 111 32 892 30 2 30 2831
Fragments arranged by size:
2831 1787 1696 892 155 111 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 3606 4745 3606
Size: 1139 6567
KasI G'GCGC_C
Cuts at: 151 433 5136 5384 151
Size: 282 4703 248 2473
Fragments arranged by size:
4703 2473 282 248
KpnI G_GTAC'C
Cuts at: 4726 4726
Size: 7706
MluI A'CGCG_T
Cuts at: 7139 7139
Size: 7706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 1706 2135 2472 2711 3678 3820 6377 6561
Size: 915 429 337 239 967 142 2557 184
Cuts at: 6561 791
Size: 1936
Fragments arranged by size:
2557 1936 967 915 429 337 239 184
142
MscI TGG'CCA
Cuts at: 836 2239 2869 3685 5467 836
Size: 1403 630 816 1782 3075
Fragments arranged by size:
3075 1782 1403 816 630
MslI CAynn'nnrTG
Cuts at: 358 598 1443 1669 2317 2488 2621 3041 3056
Size: 240 845 226 648 171 133 420 15
Cuts at: 3056 3157 3532 4293 5695 5822 358
Size: 101 375 761 1402 127 2242
Fragments arranged by size:
2242 1402 845 761 648 420 375 240
226 171 133 127 101 15
MspA1I CmG'CkG
Cuts at: 250 587 635 1272 1514 1577 1902 2286 2774
Size: 337 48 637 242 63 325 384 488
Cuts at: 2774 2945 3068 3099 3302 3326 3557 4817 5131
Size: 171 123 31 203 24 231 1260 314
Cuts at: 5131 5491 6504 6749 250
Size: 360 1013 245 1207
Fragments arranged by size:
1260 1207 1013 637 488 384 360 337
325 314 245 242 231 203 171 123
63 48 31 24
NaeI GCC'GGC
Cuts at: 2004 3576 5887 2004
Size: 1572 2311 3823
Fragments arranged by size:
3823 2311 1572
NarI GG'CG_CC
Cuts at: 152 434 5137 5385 152
Size: 282 4703 248 2473
Fragments arranged by size:
4703 2473 282 248
NcoI C'CATG_G
Cuts at: 125 2616 2792 3467 4260 4380 125
Size: 2491 176 675 793 120 3451
Fragments arranged by size:
3451 2491 793 675 176 120
NgoMIV G'CCGG_C
Cuts at: 2002 3574 5885 2002
Size: 1572 2311 3823
Fragments arranged by size:
3823 2311 1572
NsiI A_TGCA'T
Cuts at: 4042 4521 4042
Size: 479 7227
NspI r_CATG'y
Cuts at: 738 2376 2493 2520 2918 3419 4296 4759 5259
Size: 1638 117 27 398 501 877 463 500
Cuts at: 5259 6166 738
Size: 907 2278
Fragments arranged by size:
2278 1638 907 877 501 500 463 398
117 27
PasI CC'CwG_GG
Cuts at: 2062 3429 4101 2062
Size: 1367 672 5667
Fragments arranged by size:
5667 1367 672
PciI A'CATG_T
Cuts at: 2516 4292 6162 2516
Size: 1776 1870 4060
Fragments arranged by size:
4060 1870 1776
PflMI CCAn_nnn'nTGG
Cuts at: 273 1692 2235 3393 273
Size: 1419 543 1158 4586
Fragments arranged by size:
4586 1419 1158 543
PfoI T'CCnGG_A
Cuts at: 440 1481 2228 2243 440
Size: 1041 747 15 5903
Fragments arranged by size:
5903 1041 747 15
PmeI GTTT'AAAC
Cuts at: 4705 4705
Size: 7706
PmlI CAC'GTG
Cuts at: 980 3762 980
Size: 2782 4924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6845 6877 6845
Size: 32 7674
PpuMI rG'GwC_Cy
Cuts at: 444 1326 2925 4233 4283 444
Size: 882 1599 1308 50 3867
Fragments arranged by size:
3867 1599 1308 882 50
PshAI GACnn'nnGTC
Cuts at: 2025 2714 2025
Size: 689 7017
PspOMI G'GGCC_C
Cuts at: 1508 3383 3518 4573 1508
Size: 1875 135 1055 4641
Fragments arranged by size:
4641 1875 1055 135
PstI C_TGCA'G
Cuts at: 1098 1334 1373 1406 1519 2716 2779 3482 3680
Size: 236 39 33 113 1197 63 703 198
Cuts at: 3680 4073 4753 5438 1098
Size: 393 680 685 3366
Fragments arranged by size:
3366 1197 703 685 680 393 236 198
113 63 39 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7706
PvuII CAG'CTG
Cuts at: 635 1272 1902 2286 3068 3302 5131 5491 635
Size: 637 630 384 782 234 1829 360 2850
Fragments arranged by size:
2850 1829 782 637 630 384 360 234
RsrII CG'GwC_CG
Cuts at: 4011 5901 4011
Size: 1890 5816
SacI G_AGCT'C
Cuts at: 4720 4720
Size: 7706
SacII CC_GC'GG
Cuts at: 588 588
Size: 7706
SalI G'TCGA_C
Cuts at: 4743 4743
Size: 7706
SanDI GG'GwC_CC
Cuts at: 4233 4233
Size: 7706
SapI GCTCTTCn'nnn_
Cuts at: 1396 5729 5939 1396
Size: 4333 210 3163
Fragments arranged by size:
4333 3163 210
SbfI CC_TGCA'GG
Cuts at: 1098 3680 4753 1098
Size: 2582 1073 4051
Fragments arranged by size:
4051 2582 1073
ScaI AGT'ACT
Cuts at: 7223 7223
Size: 7706
SexAI A'CCwGG_T
Cuts at: 410 410
Size: 7706
SfcI C'TryA_G
Cuts at: 33 1094 1267 1330 1369 1402 1515 2712 2775
Size: 1061 173 63 39 33 113 1197 63
Cuts at: 2775 2958 3321 3478 3676 4069 4749 5434 6427
Size: 183 363 157 198 393 680 685 993
Cuts at: 6427 6618 33
Size: 191 1121
Fragments arranged by size:
1197 1121 1061 993 685 680 393 363
198 191 183 173 157 113 63 63
39 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 1940 1940
Size: 7706
SfoI GGC'GCC
Cuts at: 153 435 5138 5386 153
Size: 282 4703 248 2473
Fragments arranged by size:
4703 2473 282 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
SmaI CCC'GGG
Cuts at: 135 4231 4728 135
Size: 4096 497 3113
Fragments arranged by size:
4096 3113 497
SmlI C'TyrA_G
Cuts at: 376 2212 4861 5776 6268 6530 376
Size: 1836 2649 915 492 262 1552
Fragments arranged by size:
2649 1836 1552 915 492 262
SnaBI TAC'GTA
Cuts at: 4698 4698
Size: 7706
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7706
SphI G_CATG'C
Cuts at: 2376 2493 4759 5259 2376
Size: 117 2266 500 4823
Fragments arranged by size:
4823 2266 500 117
StyI C'CwwG_G
Cuts at: 125 182 1238 2188 2378 2616 2792 3467 3647
Size: 57 1056 950 190 238 176 675 180
Cuts at: 3647 3960 4260 4380 4404 4538 4840 5194 125
Size: 313 300 120 24 134 302 354 2637
Fragments arranged by size:
2637 1056 950 675 354 313 302 300
238 190 180 176 134 120 57 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1123 2404 2727 2935 3480 5289 5422 5881 6090
Size: 1281 323 208 545 1809 133 459 209
Cuts at: 6090 1123
Size: 2739
Fragments arranged by size:
2739 1809 1281 545 459 323 209 208
133
TatI w'GTAC_w
Cuts at: 776 1112 3806 7221 776
Size: 336 2694 3415 1261
Fragments arranged by size:
3415 2694 1261 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 895 927 1621 1653 2163 2195 3321 3353 3455
Size: 32 694 32 510 32 1126 32 102
Cuts at: 3455 3487 4256 4288 7223 7255 895
Size: 32 769 32 2935 32 1346
Fragments arranged by size:
2935 1346 1126 769 694 510 102 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5503 5503
Size: 7706
XbaI T'CTAG_A
Cuts at: 4737 4737
Size: 7706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1689 2232 3436 5201 1689
Size: 543 1204 1765 4194
Fragments arranged by size:
4194 1765 1204 543
XmaI C'CCGG_G
Cuts at: 133 4229 4726 133
Size: 4096 497 3113
Fragments arranged by size:
4096 3113 497
XmnI GAAnn'nnTTC
Cuts at: 580 580
Size: 7706
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI BmgBI BmtI BsiWI BsmI
BsmBI BstZ17I Bsu36I ClaI CspCI EcoNI FseI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PsiI
PspXI PsrI SgrAI SrfI SspI StuI SwaI XhoI
ZraI