Restriction map for
pF1KSDA0823
(Circular) (Six-base) MAPSORT of: pF1KSDA0823.seq Check: 6687 from: 1 to: 4840
pF1KSDA0823 4840 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 562 562
Size: 4840
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4840
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4840
AclI AA'CG_TT
Cuts at: 4680 4680
Size: 4840
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 409 1594 2663 3095 3844 4112 247
Size: 162 1185 1069 432 749 268 975
Fragments arranged by size:
1185 1069 975 749 432 268 162
AflIII A'CryG_T
Cuts at: 2190 3296 4273 2190
Size: 1106 977 2757
Fragments arranged by size:
2757 1106 977
AgeI A'CCGG_T
Cuts at: 3228 3228
Size: 4840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 570 767 947 1256 1604 570
Size: 197 180 309 348 3806
Fragments arranged by size:
3806 348 309 197 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1027 1059 1027
Size: 32 4808
AlwNI CAG_nnn'CTG
Cuts at: 165 225 789 2143 3712 165
Size: 60 564 1354 1569 1293
Fragments arranged by size:
1569 1354 1293 564 60
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4840
ApaLI G'TGCA_C
Cuts at: 3610 3610
Size: 4840
ApoI r'AATT_y
Cuts at: 1354 1842 4193 4449 1354
Size: 488 2351 256 1745
Fragments arranged by size:
2351 1745 488 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4840
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4840
AvaI C'yCGr_G
Cuts at: 1858 1858
Size: 4840
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4840
BanI G'GyrC_C
Cuts at: 174 496 811 1473 1854 2268 2516 2551 174
Size: 322 315 662 381 414 248 35 2463
Fragments arranged by size:
2463 662 414 381 322 315 248 35
BanII G_rGCy'C
Cuts at: 219 866 920 1416 1555 1852 2882 219
Size: 647 54 496 139 297 1030 2177
Fragments arranged by size:
2177 1030 647 496 297 139 54
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4592
BbvCI CC'TCA_GC
Cuts at: 355 1102 1247 355
Size: 747 145 3948
Fragments arranged by size:
3948 747 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 623 589
Size: 34 4806
BciVI GTATCCnnnnn_n'
Cuts at: 838 2736 3499 4840 838
Size: 1898 763 1341 838
Fragments arranged by size:
1898 1341 838 763
BclI T'GATC_A
Cuts at: 1085 2358 1085
Size: 1273 3567
BglI GCCn_nnn'nGGC
Cuts at: 334 334
Size: 4840
BglII A'GATC_T
Cuts at: 2353 4392 2353
Size: 2039 2801
BlpI GC'TnA_GC
Cuts at: 860 1603 1950 860
Size: 743 347 3750
Fragments arranged by size:
3750 743 347
Bme1580I G_kGCm'C
Cuts at: 179 219 240 2463 2556 3614 179
Size: 40 21 2223 93 1058 1405
Fragments arranged by size:
2223 1405 1058 93 40 21
BmrI ACTGGGnnnn_n'
Cuts at: 912 2050 2131 2466 4612 912
Size: 1138 81 335 2146 1140
Fragments arranged by size:
2146 1140 1138 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1003 3205 1003
Size: 2202 2638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 205 364 854 1081 1119 1351 1551 1588
Size: 18 159 490 227 38 232 200 37
Cuts at: 1588 187
Size: 3439
Fragments arranged by size:
3439 490 232 227 200 159 38 37
18
Bpu10I CC'TnA_GC
Cuts at: 355 737 1102 1247 1618 355
Size: 382 365 145 371 3577
Fragments arranged by size:
3577 382 371 365 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1140 2014 2893 3387 3685 1140
Size: 874 879 494 298 2295
Fragments arranged by size:
2295 879 874 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 670 1282 4544 670
Size: 612 3262 966
Fragments arranged by size:
3262 966 612
BsaAI yAC'GTr
Cuts at: 138 1830 2191 138
Size: 1692 361 2787
Fragments arranged by size:
2787 1692 361
BsaBI GATnn'nnATC
Cuts at: 6 2352 4391 6
Size: 2346 2039 455
Fragments arranged by size:
2346 2039 455
BsaHI Gr'CG_yC
Cuts at: 365 1517 2269 2517 3221 4717 365
Size: 1152 752 248 704 1496 488
Fragments arranged by size:
1496 1152 752 704 488 248
BsaWI w'CCGG_w
Cuts at: 1416 2027 2548 3228 3502 3649 4060 1416
Size: 611 521 680 274 147 411 2196
Fragments arranged by size:
2196 680 611 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 872 902 1027 1057 872
Size: 30 125 30 4655
Fragments arranged by size:
4655 125 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 856 1048 1801 1810 475
Size: 381 192 753 9 3505
Fragments arranged by size:
3505 753 381 192 9
BseYI C'CCAG_C
Cuts at: 218 911 2263 3021 3600 4096 218
Size: 693 1352 758 579 496 962
Fragments arranged by size:
1352 962 758 693 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1262 808
Size: 454 4386
BsiEI CG_ry'CG
Cuts at: 104 303 2380 2426 3636 104
Size: 199 2077 46 1210 1308
Fragments arranged by size:
2077 1308 1210 199 46
BsiHKAI G_wGCw'C
Cuts at: 866 1852 2630 2820 3614 866
Size: 986 778 190 794 2092
Fragments arranged by size:
2092 986 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 179 219 240 866 920 1416 1555 1852 2463
Size: 40 21 626 54 496 139 297 611
Cuts at: 2463 2556 2630 2820 2882 3614 179
Size: 93 74 190 62 732 1405
Fragments arranged by size:
1405 732 626 611 496 297 190 139
93 74 62 54 40 21
BspEI T'CCGG_A
Cuts at: 4060 4060
Size: 4840
BspHI T'CATG_A
Cuts at: 1088 4016 1088
Size: 2928 1912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 562 688 857 1888 2102 2404 2785 152
Size: 410 126 169 1031 214 302 381 2207
Fragments arranged by size:
2207 1031 410 381 302 214 169 126
BsrBI CCG'CTC
Cuts at: 50 189 282 3130 3184 4670 4835 50
Size: 139 93 2848 54 1486 165 55
Fragments arranged by size:
2848 1486 165 139 93 55 54
BsrDI GCAATG_nn'
Cuts at: 423 1722 2750 423
Size: 1299 1028 2513
Fragments arranged by size:
2513 1299 1028
BsrFI r'CCGG_y
Cuts at: 2836 3017 3228 2836
Size: 181 211 4448
Fragments arranged by size:
4448 211 181
BssSI C'ACGA_G
Cuts at: 1373 3109 3469 1373
Size: 1736 360 2744
Fragments arranged by size:
2744 1736 360
BstAPI GCAn_nnn'nTGC
Cuts at: 255 1473 2215 255
Size: 1218 742 2880
Fragments arranged by size:
2880 1218 742
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4840
BstEII G'GTnAC_C
Cuts at: 1740 1740
Size: 4840
BstXI CCAn_nnnn'nTGG
Cuts at: 138 138
Size: 4840
BstYI r'GATC_y
Cuts at: 1 578 1367 1752 1863 2330 2353 2688 2934
Size: 577 789 385 111 467 23 335 246
Cuts at: 2934 3948 4034 4046 4392 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 789 577 467 449 385 346 335
246 111 86 23 12
BtgI C'CryG_G
Cuts at: 125 492 876 975 1004 1149 125
Size: 367 384 99 29 145 3816
Fragments arranged by size:
3816 384 367 145 99 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4496
Fragments arranged by size:
4496 181 163
BtsI GCAGTG_nn'
Cuts at: 762 817 1671 2081 762
Size: 55 854 410 3521
Fragments arranged by size:
3521 854 410 55
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4840
DraIII CAC_nnn'GTG
Cuts at: 1378 1378
Size: 4840
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3404 2544
Size: 860 3980
EaeI y'GGCC_r
Cuts at: 128 300 558 1442 1511 1643 2423 2597 2988
Size: 172 258 884 69 132 780 174 391
Cuts at: 2988 3015 4362 128
Size: 27 1347 606
Fragments arranged by size:
1347 884 780 606 391 258 174 172
132 69 27
EagI C'GGCC_G
Cuts at: 300 2423 300
Size: 2123 2717
EarI CTCTTCn'nnn_
Cuts at: 1034 2861 3071 1034
Size: 1827 210 2803
Fragments arranged by size:
2803 1827 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 3358 3504 4650 3046
Size: 312 146 1146 3236
Fragments arranged by size:
3236 1146 312 146
EcoICRI GAG'CTC
Cuts at: 864 1850 864
Size: 986 3854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 205 247 364 409 854 1081 1119 1351
Size: 18 42 117 45 445 227 38 232
Cuts at: 1351 1551 1588 1594 2663 3095 3844 4112 187
Size: 200 37 6 1069 432 749 268 915
Fragments arranged by size:
1069 915 749 445 432 268 232 227
200 117 45 42 38 37 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 343 354 646 1030 343
Size: 11 292 384 4153
Fragments arranged by size:
4153 384 292 11
EcoO109I rG'GnC_Cy
Cuts at: 786 1050 1130 1314 1677 1721 1776 1977 786
Size: 264 80 184 363 44 55 201 3649
Fragments arranged by size:
3649 363 264 201 184 80 55 44
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1134 1698 2857 1134
Size: 564 1159 3117
Fragments arranged by size:
3117 1159 564
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4840
HaeII r_GCGC'y
Cuts at: 213 2272 2520 3544 4523 213
Size: 2059 248 1024 979 530
Fragments arranged by size:
2059 1024 979 530 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1208 1240 2680 2710 2712 2742 1208
Size: 32 1440 30 2 30 3306
Fragments arranged by size:
3306 1440 32 30 30 2
HincII GTy'rAC
Cuts at: 615 1877 615
Size: 1262 3578
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4592
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4840
MluI A'CGCG_T
Cuts at: 4273 4273
Size: 4840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1160 3511 3695 1160
Size: 2351 184 2305
Fragments arranged by size:
2351 2305 184
MscI TGG'CCA
Cuts at: 130 1444 1645 2599 130
Size: 1314 201 954 2371
Fragments arranged by size:
2371 1314 954 201
MslI CAynn'nnrTG
Cuts at: 1076 2827 2954 1076
Size: 1751 127 2962
Fragments arranged by size:
2962 1751 127
MspA1I CmG'CkG
Cuts at: 162 225 778 859 931 1101 1236 1246 1257
Size: 63 553 81 72 170 135 10 11
Cuts at: 1257 1584 1748 1949 2263 2623 3638 3883 162
Size: 327 164 201 314 360 1015 245 1119
Fragments arranged by size:
1119 1015 553 360 327 314 245 201
170 164 135 81 72 63 11 10
NaeI GCC'GGC
Cuts at: 3019 3019
Size: 4840
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4592
NcoI C'CATG_G
Cuts at: 125 492 876 975 1004 125
Size: 367 384 99 29 3961
Fragments arranged by size:
3961 384 367 99 29
NdeI CA'TA_TG
Cuts at: 636 636
Size: 4840
NgoMIV G'CCGG_C
Cuts at: 3017 3017
Size: 4840
NotI GC'GGCC_GC
Cuts at: 300 300
Size: 4840
NruI TCG'CGA
Cuts at: 3201 3201
Size: 4840
NspI r_CATG'y
Cuts at: 633 928 1392 1891 2391 3300 633
Size: 295 464 499 500 909 2173
Fragments arranged by size:
2173 909 500 499 464 295
PasI CC'CwG_GG
Cuts at: 807 807
Size: 4840
PciI A'CATG_T
Cuts at: 3296 3296
Size: 4840
PflMI CCAn_nnn'nTGG
Cuts at: 789 1229 789
Size: 440 4400
PfoI T'CCnGG_A
Cuts at: 869 869
Size: 4840
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4840
PmlI CAC'GTG
Cuts at: 138 138
Size: 4840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1027 1059 3979 4011 1027
Size: 32 2920 32 1856
Fragments arranged by size:
2920 1856 32 32
PpuMI rG'GwC_Cy
Cuts at: 786 1050 1130 1314 1721 786
Size: 264 80 184 407 3905
Fragments arranged by size:
3905 407 264 184 80
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4840
PstI C_TGCA'G
Cuts at: 161 549 1593 1885 2570 161
Size: 388 1044 292 685 2431
Fragments arranged by size:
2431 1044 685 388 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4840
PvuII CAG'CTG
Cuts at: 162 225 859 931 1101 1246 2263 2623 162
Size: 63 634 72 170 145 1017 360 2379
Fragments arranged by size:
2379 1017 634 360 170 145 72 63
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4840
SacI G_AGCT'C
Cuts at: 866 1852 866
Size: 986 3854
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4840
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4630
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4840
ScaI AGT'ACT
Cuts at: 4357 4357
Size: 4840
SexAI A'CCwGG_T
Cuts at: 569 569
Size: 4840
SfcI C'TryA_G
Cuts at: 33 157 545 1482 1589 1881 2566 3561 3752
Size: 124 388 937 107 292 685 995 191
Cuts at: 3752 33
Size: 1121
Fragments arranged by size:
1121 995 937 685 388 292 191 124
107
SfiI GGCCn_nnn'nGGCC
Cuts at: 334 334
Size: 4840
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4592
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4840
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4840
SmlI C'TyrA_G
Cuts at: 1119 1993 2908 3402 3664 1119
Size: 874 915 494 262 2295
Fragments arranged by size:
2295 915 874 494 262
SnaBI TAC'GTA
Cuts at: 1830 1830
Size: 4840
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4840
SphI G_CATG'C
Cuts at: 928 1891 2391 928
Size: 963 500 3377
Fragments arranged by size:
3377 963 500
StuI AGG'CCT
Cuts at: 349 349
Size: 4840
StyI C'CwwG_G
Cuts at: 125 492 512 876 975 1004 1972 2326 125
Size: 367 20 364 99 29 968 354 2639
Fragments arranged by size:
2639 968 367 364 354 99 29 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 2421 2554 3013 3224 718
Size: 1703 133 459 211 2334
Fragments arranged by size:
2334 1703 459 211 133
TatI w'GTAC_w
Cuts at: 4355 4355
Size: 4840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 1099 1131 4357 4389 872
Size: 32 195 32 3226 32 1323
Fragments arranged by size:
3226 1323 195 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4840
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1788 2333 1788
Size: 545 4295
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4840
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NsiI PacI PshAI PsiI
PspXI PsrI SacII SanDI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI