Restriction map for
pF1KSDA0827
(Circular) (Six-base) MAPSORT of: pF1KSDA0827.seq Check: 2855 from: 1 to: 7730
pF1KSDA0827 7730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2953 4768 2953
Size: 1815 5915
Acc65I G'GTAC_C
Cuts at: 714 1010 3235 4746 714
Size: 296 2225 1511 3698
Fragments arranged by size:
3698 2225 1511 296
AclI AA'CG_TT
Cuts at: 7570 7570
Size: 7730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 657 991 2422 2537 2786 2870 2887 3497
Size: 340 334 1431 115 249 84 17 610
Cuts at: 3497 3559 3619 4117 5555 5987 6734 7002 317
Size: 62 60 498 1438 432 747 268 1045
Fragments arranged by size:
1438 1431 1045 747 610 498 432 340
334 268 249 115 84 62 60 17
AflIII A'CryG_T
Cuts at: 2747 5082 6186 7163 2747
Size: 2335 1104 977 3314
Fragments arranged by size:
3314 2335 1104 977
AgeI A'CCGG_T
Cuts at: 6118 6118
Size: 7730
AhdI GACnn_n'nnGTC
Cuts at: 3531 3531
Size: 7730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 1836 2621 448
Size: 1388 785 5557
Fragments arranged by size:
5557 1388 785
AlwNI CAG_nnn'CTG
Cuts at: 1600 1768 2203 2457 3010 5035 6602 1600
Size: 168 435 254 553 2025 1567 2728
Fragments arranged by size:
2728 2025 1567 553 435 254 168
ApaLI G'TGCA_C
Cuts at: 6500 6500
Size: 7730
ApoI r'AATT_y
Cuts at: 2633 3300 4346 4428 4617 4734 7083 7339 2633
Size: 667 1046 82 189 117 2349 256 3024
Fragments arranged by size:
3024 2349 1046 667 256 189 117 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 7730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7730
AvaI C'yCGr_G
Cuts at: 4750 4750
Size: 7730
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 698 731 2294 2327 4198 4231 698
Size: 33 1563 33 1871 33 4197
Fragments arranged by size:
4197 1871 1563 33 33 33
BamHI G'GATC_C
Cuts at: 4755 4755
Size: 7730
BanI G'GyrC_C
Cuts at: 714 1010 3235 4746 5160 5408 5443 714
Size: 296 2225 1511 414 248 35 3001
Fragments arranged by size:
3001 2225 1511 414 296 248 35
BanII G_rGCy'C
Cuts at: 925 1008 3511 3775 4744 5774 925
Size: 83 2503 264 969 1030 2881
Fragments arranged by size:
2881 2503 1030 969 264 83
BbeI G_GCGC'C
Cuts at: 5164 5412 5164
Size: 248 7482
BbsI GAAGACnn'nnnn_
Cuts at: 3308 3308
Size: 7730
BbvCI CC'TCA_GC
Cuts at: 453 453
Size: 7730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 988 1022 988
Size: 34 7696
BciVI GTATCCnnnnn_n'
Cuts at: 5628 6389 7730 5628
Size: 761 1341 5628
Fragments arranged by size:
5628 1341 761
BclI T'GATC_A
Cuts at: 4527 5250 4527
Size: 723 7007
BglII A'GATC_T
Cuts at: 1720 1975 5245 7282 1720
Size: 255 3270 2037 2168
Fragments arranged by size:
3270 2168 2037 255
BlpI GC'TnA_GC
Cuts at: 151 4842 151
Size: 4691 3039
Bme1580I G_kGCm'C
Cuts at: 5355 5448 6504 5355
Size: 93 1056 6581
Fragments arranged by size:
6581 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 4146 4942 5023 5358 7502 4146
Size: 796 81 335 2144 4374
Fragments arranged by size:
4374 2144 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 465 3875 6097 312
Size: 153 3410 2222 1945
Fragments arranged by size:
3410 2222 1945 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 929 1736 2555 4241 290
Size: 639 807 819 1686 3779
Fragments arranged by size:
3779 1686 819 807 639
Bpu10I CC'TnA_GC
Cuts at: 453 453
Size: 7730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3374 3506 4211 4906 5785 6277 6575 3167
Size: 207 132 705 695 879 492 298 4322
Fragments arranged by size:
4322 879 705 695 492 298 207 132
BsaI GGTCTCn'nnnn_
Cuts at: 2137 7434 2137
Size: 5297 2433
BsaAI yAC'GTr
Cuts at: 4722 5083 4722
Size: 361 7369
BsaBI GATnn'nnATC
Cuts at: 6 624 5244 7281 6
Size: 618 4620 2037 455
Fragments arranged by size:
4620 2037 618 455
BsaHI Gr'CG_yC
Cuts at: 3964 5161 5409 6111 7607 3964
Size: 1197 248 702 1496 4087
Fragments arranged by size:
4087 1496 1197 702 248
BsaWI w'CCGG_w
Cuts at: 4919 5440 6118 6392 6539 6950 4919
Size: 521 678 274 147 411 5699
Fragments arranged by size:
5699 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 1734 1764 217
Size: 30 1487 30 6183
Fragments arranged by size:
6183 1487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 333 441 2987 3438 4181 4304 293
Size: 40 108 2546 451 743 123 3719
Fragments arranged by size:
3719 2546 743 451 123 108 40
BseYI C'CCAG_C
Cuts at: 1758 5155 5913 6490 6986 1758
Size: 3397 758 577 496 2502
Fragments arranged by size:
3397 2502 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2627
Size: 7730
BsiEI CG_ry'CG
Cuts at: 104 5272 5318 6526 104
Size: 5168 46 1208 1308
Fragments arranged by size:
5168 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 688 1008 4744 5522 5712 6504 688
Size: 320 3736 778 190 792 1914
Fragments arranged by size:
3736 1914 792 778 320 190
BsmI GAATG_Cn'
Cuts at: 1305 4475 1305
Size: 3170 4560
BsmBI CGTCTCn'nnnn_
Cuts at: 3971 3971
Size: 7730
Bsp1286I G_dGCh'C
Cuts at: 688 925 1008 3511 3775 4744 5355 5448 5522
Size: 237 83 2503 264 969 611 93 74
Cuts at: 5522 5712 5774 6504 688
Size: 190 62 730 1914
Fragments arranged by size:
2503 1914 969 730 611 264 237 190
93 83 74 62
BspEI T'CCGG_A
Cuts at: 6950 6950
Size: 7730
BspHI T'CATG_A
Cuts at: 1382 1659 1725 6906 1382
Size: 277 66 5181 2206
Fragments arranged by size:
5181 2206 277 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3033 4780 4994 5296 5677 3033
Size: 1747 214 302 381 5086
Fragments arranged by size:
5086 1747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6022 6076 7560 7725 50
Size: 5972 54 1484 165 55
Fragments arranged by size:
5972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 145 1853 2816 3491 4519 4681 5642 145
Size: 1708 963 675 1028 162 961 2233
Fragments arranged by size:
2233 1708 1028 963 961 675 162
BsrFI r'CCGG_y
Cuts at: 5728 5909 6118 5728
Size: 181 209 7340
Fragments arranged by size:
7340 209 181
BsrGI T'GTAC_A
Cuts at: 551 981 551
Size: 430 7300
BssSI C'ACGA_G
Cuts at: 1352 6001 6359 1352
Size: 4649 358 2723
Fragments arranged by size:
4649 2723 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 2203 3607 5107 1771
Size: 432 1404 1500 4394
Fragments arranged by size:
4394 1500 1404 432
BstBI TT'CG_AA
Cuts at: 110 6091 110
Size: 5981 1749
BstEII G'GTnAC_C
Cuts at: 4214 4214
Size: 7730
BstYI r'GATC_y
Cuts at: 1 1720 1975 4755 5222 5245 5580 5826 6838
Size: 1719 255 2780 467 23 335 246 1012
Cuts at: 6838 6924 6936 7282 1
Size: 86 12 346 449
Fragments arranged by size:
2780 1719 1012 467 449 346 335 255
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 569 3758 569
Size: 3189 4541
BtgI C'CryG_G
Cuts at: 353 458 848 1029 3146 3950 353
Size: 105 390 181 2117 804 4133
Fragments arranged by size:
4133 2117 804 390 181 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2243 5680 5861 6024 2243
Size: 3437 181 163 3949
Fragments arranged by size:
3949 3437 181 163
BtsI GCAGTG_nn'
Cuts at: 1408 2505 2983 4973 1408
Size: 1097 478 1990 4165
Fragments arranged by size:
4165 1990 1097 478
DraI TTT'AAA
Cuts at: 4729 4729
Size: 7730
DraIII CAC_nnn'GTG
Cuts at: 831 831
Size: 7730
DrdI GACnn_nn'nnGTC
Cuts at: 2001 5436 6294 2001
Size: 3435 858 3437
Fragments arranged by size:
3437 3435 858
EaeI y'GGCC_r
Cuts at: 4538 5315 5489 5880 5907 7252 4538
Size: 777 174 391 27 1345 5016
Fragments arranged by size:
5016 1345 777 391 174 27
EagI C'GGCC_G
Cuts at: 5315 5315
Size: 7730
EarI CTCTTCn'nnn_
Cuts at: 351 390 529 913 1428 1826 2709 3516 4252
Size: 39 139 384 515 398 883 807 736
Cuts at: 4252 5753 5963 351
Size: 1501 210 2118
Fragments arranged by size:
2118 1501 883 807 736 515 398 384
210 139 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5938 6248 6394 7540 5938
Size: 310 146 1146 6128
Fragments arranged by size:
6128 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1006 4742 1006
Size: 3736 3994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 317 657 929 991 1736 2422 2537 2555
Size: 27 340 272 62 745 686 115 18
Cuts at: 2555 2786 2870 2887 3497 3559 3619 4117 4241
Size: 231 84 17 610 62 60 498 124
Cuts at: 4241 5555 5987 6734 7002 290
Size: 1314 432 747 268 1018
Fragments arranged by size:
1314 1018 747 745 686 610 498 432
340 272 268 231 124 115 84 62
62 60 27 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1018 3379 1018
Size: 2361 5369
EcoO109I rG'GnC_Cy
Cuts at: 4144 4869 4144
Size: 725 7005
EcoRI G'AATT_C
Cuts at: 4346 4428 4617 4734 4346
Size: 82 189 117 7342
Fragments arranged by size:
7342 189 117 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1255 1494 2617 3208 5749 1255
Size: 239 1123 591 2541 3236
Fragments arranged by size:
3236 2541 1123 591 239
FspI TGC'GCA
Cuts at: 5511 5511
Size: 7730
HaeII r_GCGC'y
Cuts at: 5164 5412 6434 7413 5164
Size: 248 1022 979 5481
Fragments arranged by size:
5481 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 157 159 188 189 220 649 681 1364
Size: 30 2 29 1 31 429 32 683
Cuts at: 1364 1396 1567 1599 3268 3300 3517 3549 5572
Size: 32 171 32 1669 32 217 32 2023
Cuts at: 5572 5602 5604 5634 127
Size: 30 2 30 2223
Fragments arranged by size:
2223 2023 1669 683 429 217 171 32
32 32 32 32 31 30 30 30
29 2 2 1
HincII GTy'rAC
Cuts at: 497 2511 3189 3536 3641 4769 497
Size: 2014 678 347 105 1128 3458
Fragments arranged by size:
3458 2014 1128 678 347 105
HindIII A'AGCT_T
Cuts at: 1476 1476
Size: 7730
HpaI GTT'AAC
Cuts at: 497 3189 497
Size: 2692 5038
KasI G'GCGC_C
Cuts at: 5160 5408 5160
Size: 248 7482
KpnI G_GTAC'C
Cuts at: 718 1014 3239 4750 718
Size: 296 2225 1511 3698
Fragments arranged by size:
3698 2225 1511 296
MluI A'CGCG_T
Cuts at: 7163 7163
Size: 7730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 571 692 1989 2544 3696 4136 6401 6585 571
Size: 121 1297 555 1152 440 2265 184 1716
Fragments arranged by size:
2265 1716 1297 1152 555 440 184 121
MscI TGG'CCA
Cuts at: 4540 5491 4540
Size: 951 6779
MslI CAynn'nnrTG
Cuts at: 3064 3496 5719 5846 3064
Size: 432 2223 127 4948
Fragments arranged by size:
4948 2223 432 127
MspA1I CmG'CkG
Cuts at: 310 1768 2217 2457 2848 4841 5155 5515 6528
Size: 1458 449 240 391 1993 314 360 1013
Cuts at: 6528 6773 310
Size: 245 1267
Fragments arranged by size:
1993 1458 1267 1013 449 391 360 314
245 240
NaeI GCC'GGC
Cuts at: 5911 5911
Size: 7730
NarI GG'CG_CC
Cuts at: 5161 5409 5161
Size: 248 7482
NcoI C'CATG_G
Cuts at: 353 3146 3950 353
Size: 2793 804 4133
Fragments arranged by size:
4133 2793 804
NgoMIV G'CCGG_C
Cuts at: 5909 5909
Size: 7730
NruI TCG'CGA
Cuts at: 195 195
Size: 7730
NspI r_CATG'y
Cuts at: 4341 4783 5283 6190 4341
Size: 442 500 907 5881
Fragments arranged by size:
5881 907 500 442
PasI CC'CwG_GG
Cuts at: 3768 3768
Size: 7730
PciI A'CATG_T
Cuts at: 6186 6186
Size: 7730
PmeI GTTT'AAAC
Cuts at: 4729 4729
Size: 7730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 675 707 6869 6901 675
Size: 32 6162 32 1504
Fragments arranged by size:
6162 1504 32 32
PpuMI rG'GwC_Cy
Cuts at: 4144 4144
Size: 7730
PshAI GACnn'nnGTC
Cuts at: 3148 3148
Size: 7730
PstI C_TGCA'G
Cuts at: 643 1168 3006 4427 4777 5462 643
Size: 525 1838 1421 350 685 2911
Fragments arranged by size:
2911 1838 1421 685 525 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7730
PvuII CAG'CTG
Cuts at: 310 1768 2217 2457 2848 5155 5515 310
Size: 1458 449 240 391 2307 360 2525
Fragments arranged by size:
2525 2307 1458 449 391 360 240
RsrII CG'GwC_CG
Cuts at: 5925 5925
Size: 7730
SacI G_AGCT'C
Cuts at: 1008 4744 1008
Size: 3736 3994
SalI G'TCGA_C
Cuts at: 4767 4767
Size: 7730
SapI GCTCTTCn'nnn_
Cuts at: 913 2709 3516 5753 5963 913
Size: 1796 807 2237 210 2680
Fragments arranged by size:
2680 2237 1796 807 210
SbfI CC_TGCA'GG
Cuts at: 4777 4777
Size: 7730
ScaI AGT'ACT
Cuts at: 2484 7247 2484
Size: 4763 2967
SfcI C'TryA_G
Cuts at: 33 257 639 1067 1097 1164 3002 4423 4488
Size: 224 382 428 30 67 1838 1421 65
Cuts at: 4488 4773 5458 6451 6642 33
Size: 285 685 993 191 1121
Fragments arranged by size:
1838 1421 1121 993 685 428 382 285
224 191 67 65 30
SfoI GGC'GCC
Cuts at: 5162 5410 5162
Size: 248 7482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7730
SmaI CCC'GGG
Cuts at: 4752 4752
Size: 7730
SmlI C'TyrA_G
Cuts at: 3182 3389 3521 4226 4885 5800 6292 6554 3182
Size: 207 132 705 659 915 492 262 4358
Fragments arranged by size:
4358 915 705 659 492 262 207 132
SnaBI TAC'GTA
Cuts at: 4722 4722
Size: 7730
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7730
SphI G_CATG'C
Cuts at: 4783 5283 4783
Size: 500 7230
SspI AAT'ATT
Cuts at: 1884 2406 1884
Size: 522 7208
StuI AGG'CCT
Cuts at: 1162 1162
Size: 7730
StyI C'CwwG_G
Cuts at: 353 2126 3146 3950 4692 4864 5218 353
Size: 1773 1020 804 742 172 354 2865
Fragments arranged by size:
2865 1773 1020 804 742 354 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5313 5446 5905 6114 5313
Size: 133 459 209 6929
Fragments arranged by size:
6929 459 209 133
TatI w'GTAC_w
Cuts at: 551 981 1438 2482 7245 551
Size: 430 457 1044 4763 1036
Fragments arranged by size:
4763 1044 1036 457 430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 598 630 1734 1766 7247 7279 598
Size: 32 1104 32 5481 32 1049
Fragments arranged by size:
5481 1104 1049 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2515 5527 2515
Size: 3012 4718
XbaI T'CTAG_A
Cuts at: 244 2278 4761 244
Size: 2034 2483 3213
Fragments arranged by size:
3213 2483 2034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 904 3957 5225 904
Size: 3053 1268 3409
Fragments arranged by size:
3409 3053 1268
XmaI C'CCGG_G
Cuts at: 4750 4750
Size: 7730
XmnI GAAnn'nnTTC
Cuts at: 1619 2661 2726 1619
Size: 1042 65 6623
Fragments arranged by size:
6623 1042 65
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BfrBI BglI BmgBI BmtI BsiWI BssHII BstXI
BstZ17I ClaI CspCI EcoRV FseI FspAI MfeI NdeI
NheI NotI NsiI PacI PflMI PfoI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI