Restriction map for
pF1KSDA0828
(Circular) (Six-base) MAPSORT of: pF1KSDA0828.seq Check: 1992 from: 1 to: 4969
pF1KSDA0828 4969 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1862 1862
Size: 4969
AatII G_ACGT'C
Cuts at: 546 546
Size: 4969
AccI GT'mk_AC
Cuts at: 1808 2005 1808
Size: 197 4772
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4969
AclI AA'CG_TT
Cuts at: 4809 4809
Size: 4969
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 2792 3224 3973 4241 202
Size: 2590 432 749 268 930
Fragments arranged by size:
2590 930 749 432 268
AflIII A'CryG_T
Cuts at: 1094 2319 3425 4402 1094
Size: 1225 1106 977 1661
Fragments arranged by size:
1661 1225 1106 977
AgeI A'CCGG_T
Cuts at: 3357 3357
Size: 4969
AlwNI CAG_nnn'CTG
Cuts at: 2272 3841 2272
Size: 1569 3400
ApaI G_GGCC'C
Cuts at: 332 876 1931 332
Size: 544 1055 3370
Fragments arranged by size:
3370 1055 544
ApaLI G'TGCA_C
Cuts at: 3739 3739
Size: 4969
ApoI r'AATT_y
Cuts at: 514 1222 1971 4322 4578 514
Size: 708 749 2351 256 905
Fragments arranged by size:
2351 905 749 708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4969
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4969
AvaI C'yCGr_G
Cuts at: 318 324 1987 318
Size: 6 1663 3300
Fragments arranged by size:
3300 1663 6
BamHI G'GATC_C
Cuts at: 1992 1992
Size: 4969
BanI G'GyrC_C
Cuts at: 165 241 363 416 454 468 1983 2397 2645
Size: 76 122 53 38 14 1515 414 248
Cuts at: 2645 2680 165
Size: 35 2454
Fragments arranged by size:
2454 1515 414 248 122 76 53 38
35 14
BanII G_rGCy'C
Cuts at: 196 325 332 777 876 1369 1931 1981 3011
Size: 129 7 445 99 493 562 50 1030
Cuts at: 3011 196
Size: 2154
Fragments arranged by size:
2154 1030 562 493 445 129 99 50
7
BbeI G_GCGC'C
Cuts at: 245 472 2401 2649 245
Size: 227 1929 248 2565
Fragments arranged by size:
2565 1929 248 227
BciVI GTATCCnnnnn_n'
Cuts at: 1097 2865 3628 4969 1097
Size: 1768 763 1341 1097
Fragments arranged by size:
1768 1341 1097 763
BclI T'GATC_A
Cuts at: 2487 2487
Size: 4969
BglI GCCn_nnn'nGGC
Cuts at: 256 452 256
Size: 196 4773
BglII A'GATC_T
Cuts at: 1624 2482 4521 1624
Size: 858 2039 2072
Fragments arranged by size:
2072 2039 858
BlpI GC'TnA_GC
Cuts at: 594 2079 594
Size: 1485 3484
Bme1580I G_kGCm'C
Cuts at: 332 876 1407 1931 2592 2685 3743 332
Size: 544 531 524 661 93 1058 1558
Fragments arranged by size:
1558 1058 661 544 531 524 93
BmgBI CAC'GTC
Cuts at: 1581 1581
Size: 4969
BmrI ACTGGGnnnn_n'
Cuts at: 870 2179 2260 2595 4741 870
Size: 1309 81 335 2146 1098
Fragments arranged by size:
2146 1309 1098 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 4969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 818 1157 266
Size: 552 339 4078
Fragments arranged by size:
4078 552 339
Bpu10I CC'TnA_GC
Cuts at: 190 1699 190
Size: 1509 3460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1613 2143 3022 3516 3814 761
Size: 852 530 879 494 298 1916
Fragments arranged by size:
1916 879 852 530 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 263 4673 263
Size: 4410 559
BsaAI yAC'GTr
Cuts at: 1959 2320 1959
Size: 361 4608
BsaBI GATnn'nnATC
Cuts at: 6 2481 4520 6
Size: 2475 2039 455
Fragments arranged by size:
2475 2039 455
BsaHI Gr'CG_yC
Cuts at: 242 373 469 543 2398 2646 3350 4846 242
Size: 131 96 74 1855 248 704 1496 365
Fragments arranged by size:
1855 1496 704 365 248 131 96 74
BsaWI w'CCGG_w
Cuts at: 2156 2677 3357 3631 3778 4189 2156
Size: 521 680 274 147 411 2936
Fragments arranged by size:
2936 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 4969
BseYI C'CCAG_C
Cuts at: 1168 2392 3150 3729 4225 1168
Size: 1224 758 579 496 1912
Fragments arranged by size:
1912 1224 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 154
Size: 4969
BsiEI CG_ry'CG
Cuts at: 104 233 2509 2555 3765 104
Size: 129 2276 46 1210 1308
Fragments arranged by size:
2276 1308 1210 129 46
BsiHKAI G_wGCw'C
Cuts at: 229 777 867 1517 1730 1981 2759 2949 3743
Size: 548 90 650 213 251 778 190 794
Cuts at: 3743 229
Size: 1455
Fragments arranged by size:
1455 794 778 650 548 251 213 190
90
Bsp1286I G_dGCh'C
Cuts at: 196 229 325 332 777 867 876 1369 1407
Size: 33 96 7 445 90 9 493 38
Cuts at: 1407 1517 1730 1931 1981 2592 2685 2759 2949
Size: 110 213 201 50 611 93 74 190
Cuts at: 2949 3011 3743 196
Size: 62 732 1422
Fragments arranged by size:
1422 732 611 493 445 213 201 190
110 96 93 90 74 62 50 38
33 9 7
BspEI T'CCGG_A
Cuts at: 4189 4189
Size: 4969
BspHI T'CATG_A
Cuts at: 4145 4145
Size: 4969
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 1862 2017 2231 2533 2914 126
Size: 1736 155 214 302 381 2181
Fragments arranged by size:
2181 1736 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 300 340 3259 3313 4799 4964 50
Size: 250 40 2919 54 1486 165 55
Fragments arranged by size:
2919 1486 250 165 55 54 40
BsrDI GCAATG_nn'
Cuts at: 2879 2879
Size: 4969
BsrFI r'CCGG_y
Cuts at: 2965 3146 3357 2965
Size: 181 211 4577
Fragments arranged by size:
4577 211 181
BsrGI T'GTAC_A
Cuts at: 1483 1483
Size: 4969
BssSI C'ACGA_G
Cuts at: 3238 3598 3238
Size: 360 4609
BstAPI GCAn_nnn'nTGC
Cuts at: 2344 2344
Size: 4969
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4969
BstEII G'GTnAC_C
Cuts at: 1503 1503
Size: 4969
BstXI CCAn_nnnn'nTGG
Cuts at: 810 1827 1908 810
Size: 1017 81 3871
Fragments arranged by size:
3871 1017 81
BstYI r'GATC_y
Cuts at: 1 488 1059 1624 1992 2459 2482 2817 3063
Size: 487 571 565 368 467 23 335 246
Cuts at: 3063 4077 4163 4175 4521 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 571 565 487 467 449 368 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 170 275 368 1784 170
Size: 105 93 1416 3355
Fragments arranged by size:
3355 1416 105 93
BtgI C'CryG_G
Cuts at: 149 236 245 293 1361 149
Size: 87 9 48 1068 3757
Fragments arranged by size:
3757 1068 87 48 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 617 2917 3098 3261 617
Size: 2300 181 163 2325
Fragments arranged by size:
2325 2300 181 163
BtsI GCAGTG_nn'
Cuts at: 678 827 1736 2210 678
Size: 149 909 474 3437
Fragments arranged by size:
3437 909 474 149
ClaI AT'CG_AT
Cuts at: 1001 1001
Size: 4969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 519 554 519
Size: 35 4934
DraI TTT'AAA
Cuts at: 1966 1966
Size: 4969
DrdI GACnn_nn'nnGTC
Cuts at: 457 2673 3533 457
Size: 2216 860 1893
Fragments arranged by size:
2216 1893 860
EaeI y'GGCC_r
Cuts at: 230 377 1841 2552 2726 3117 3144 4491 230
Size: 147 1464 711 174 391 27 1347 708
Fragments arranged by size:
1464 1347 711 708 391 174 147 27
EagI C'GGCC_G
Cuts at: 230 2552 230
Size: 2322 2647
EarI CTCTTCn'nnn_
Cuts at: 711 763 2990 3200 711
Size: 52 2227 210 2480
Fragments arranged by size:
2480 2227 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 3175 3487 3633 4779 220
Size: 2955 312 146 1146 410
Fragments arranged by size:
2955 1146 410 312 146
EcoICRI GAG'CTC
Cuts at: 775 1979 775
Size: 1204 3765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 266 818 1157 2792 3224 3973 4241 202
Size: 64 552 339 1635 432 749 268 930
Fragments arranged by size:
1635 930 749 552 432 339 268 64
EcoO109I rG'GnC_Cy
Cuts at: 186 328 435 872 1928 2106 186
Size: 142 107 437 1056 178 3049
Fragments arranged by size:
3049 1056 437 178 142 107
EcoRI G'AATT_C
Cuts at: 514 1971 514
Size: 1457 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4969
FspI TGC'GCA
Cuts at: 2748 2748
Size: 4969
HaeII r_GCGC'y
Cuts at: 245 472 2401 2649 3673 4652 245
Size: 227 1929 248 1024 979 562
Fragments arranged by size:
1929 1024 979 562 248 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 616 648 791 823 2809 2839 2841
Size: 32 391 32 143 32 1986 30 2
Cuts at: 2841 2871 193
Size: 30 2291
Fragments arranged by size:
2291 1986 391 143 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1635 2006 1635
Size: 371 4598
KasI G'GCGC_C
Cuts at: 241 468 2397 2645 241
Size: 227 1929 248 2565
Fragments arranged by size:
2565 1929 248 227
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4969
MluI A'CGCG_T
Cuts at: 4402 4402
Size: 4969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1482 1589 3640 3824 1482
Size: 107 2051 184 2627
Fragments arranged by size:
2627 2051 184 107
MscI TGG'CCA
Cuts at: 1843 2728 1843
Size: 885 4084
MslI CAynn'nnrTG
Cuts at: 130 2956 3083 130
Size: 2826 127 2016
Fragments arranged by size:
2826 2016 127
MspA1I CmG'CkG
Cuts at: 151 295 414 598 2078 2392 2752 3767 4012
Size: 144 119 184 1480 314 360 1015 245
Cuts at: 4012 151
Size: 1108
Fragments arranged by size:
1480 1108 1015 360 314 245 184 144
119
NaeI GCC'GGC
Cuts at: 3148 3148
Size: 4969
NarI GG'CG_CC
Cuts at: 242 469 2398 2646 242
Size: 227 1929 248 2565
Fragments arranged by size:
2565 1929 248 227
NcoI C'CATG_G
Cuts at: 245 1361 245
Size: 1116 3853
NgoMIV G'CCGG_C
Cuts at: 3146 3146
Size: 4969
NruI TCG'CGA
Cuts at: 3330 3330
Size: 4969
NspI r_CATG'y
Cuts at: 1098 2020 2520 3429 1098
Size: 922 500 909 2638
Fragments arranged by size:
2638 922 909 500
PasI CC'CwG_GG
Cuts at: 638 638
Size: 4969
PciI A'CATG_T
Cuts at: 1094 3425 1094
Size: 2331 2638
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4108 4140 4108
Size: 32 4937
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 4969
PsiI TTA'TAA
Cuts at: 1079 1079
Size: 4969
PspOMI G'GGCC_C
Cuts at: 328 872 1927 328
Size: 544 1055 3370
Fragments arranged by size:
3370 1055 544
PstI C_TGCA'G
Cuts at: 2014 2699 2014
Size: 685 4284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4969
PvuII CAG'CTG
Cuts at: 2392 2752 2392
Size: 360 4609
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 4969
SacI G_AGCT'C
Cuts at: 777 1981 777
Size: 1204 3765
SacII CC_GC'GG
Cuts at: 152 296 152
Size: 144 4825
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4969
SapI GCTCTTCn'nnn_
Cuts at: 2990 3200 2990
Size: 210 4759
SbfI CC_TGCA'GG
Cuts at: 2014 2014
Size: 4969
ScaI AGT'ACT
Cuts at: 1670 4486 1670
Size: 2816 2153
SfcI C'TryA_G
Cuts at: 33 588 1947 2010 2695 3690 3881 33
Size: 555 1359 63 685 995 191 1121
Fragments arranged by size:
1359 1121 995 685 555 191 63
SfoI GGC'GCC
Cuts at: 243 470 2399 2647 243
Size: 227 1929 248 2565
Fragments arranged by size:
2565 1929 248 227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4969
SmaI CCC'GGG
Cuts at: 320 1989 320
Size: 1669 3300
SmlI C'TyrA_G
Cuts at: 776 1628 2122 3037 3531 3793 776
Size: 852 494 915 494 262 1952
Fragments arranged by size:
1952 915 852 494 494 262
SnaBI TAC'GTA
Cuts at: 1959 1959
Size: 4969
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4969
SphI G_CATG'C
Cuts at: 2020 2520 2020
Size: 500 4469
SrfI GCCC'GGGC
Cuts at: 320 320
Size: 4969
StyI C'CwwG_G
Cuts at: 161 245 1361 2101 2455 161
Size: 84 1116 740 354 2675
Fragments arranged by size:
2675 1116 740 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 850 2550 2683 3142 3353 850
Size: 1700 133 459 211 2466
Fragments arranged by size:
2466 1700 459 211 133
TatI w'GTAC_w
Cuts at: 1483 1668 4484 1483
Size: 185 2816 1968
Fragments arranged by size:
2816 1968 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1164 1196 1289 1321 4486 4518 1164
Size: 32 93 32 3165 32 1615
Fragments arranged by size:
3165 1615 93 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1207 2764 1207
Size: 1557 3412
XbaI T'CTAG_A
Cuts at: 1998 1998
Size: 4969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1515 1607 2462 1515
Size: 92 855 4022
Fragments arranged by size:
4022 855 92
XmaI C'CCGG_G
Cuts at: 318 1987 318
Size: 1669 3300
XmnI GAAnn'nnTTC
Cuts at: 1253 1253
Size: 4969
ZraI GAC'GTC
Cuts at: 544 544
Size: 4969
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BsmBI BssHII BstZ17I DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PflMI PfoI PmlI PshAI PspXI PsrI SanDI
SexAI SfiI SgrAI SspI StuI SwaI XhoI