Restriction map for
pF1KSDA0831
(Circular) (Six-base) MAPSORT of: pF1KSDA0831.seq Check: 3885 from: 1 to: 4276
pF1KSDA0831 4276 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1186 1186
Size: 4276
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4276
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4276
AclI AA'CG_TT
Cuts at: 4116 4116
Size: 4276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 437 521 2099 2531 3280 3548 221
Size: 216 84 1578 432 749 268 949
Fragments arranged by size:
1578 949 749 432 268 216 84
AflII C'TTAA_G
Cuts at: 333 333
Size: 4276
AflIII A'CryG_T
Cuts at: 1626 2732 3709 1626
Size: 1106 977 2193
Fragments arranged by size:
2193 1106 977
AgeI A'CCGG_T
Cuts at: 2664 2664
Size: 4276
AhdI GACnn_n'nnGTC
Cuts at: 535 535
Size: 4276
AlwNI CAG_nnn'CTG
Cuts at: 1579 3148 1579
Size: 1569 2707
ApaI G_GGCC'C
Cuts at: 968 968
Size: 4276
ApaLI G'TGCA_C
Cuts at: 3046 3046
Size: 4276
ApoI r'AATT_y
Cuts at: 195 784 814 879 1003 1278 3629 3885 195
Size: 589 30 65 124 275 2351 256 586
Fragments arranged by size:
2351 589 586 275 256 124 65 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4276
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4276
AvaI C'yCGr_G
Cuts at: 821 1294 821
Size: 473 3803
AvrII C'CTAG_G
Cuts at: 951 951
Size: 4276
BamHI G'GATC_C
Cuts at: 1011 1299 1011
Size: 288 3988
BanI G'GyrC_C
Cuts at: 257 1290 1704 1952 1987 257
Size: 1033 414 248 35 2546
Fragments arranged by size:
2546 1033 414 248 35
BanII G_rGCy'C
Cuts at: 389 968 1288 2318 389
Size: 579 320 1030 2347
Fragments arranged by size:
2347 1030 579 320
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4028
BbsI GAAGACnn'nnnn_
Cuts at: 443 443
Size: 4276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1205 1239 1205
Size: 34 4242
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2935 4276 2172
Size: 763 1341 2172
Fragments arranged by size:
2172 1341 763
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4276
BglII A'GATC_T
Cuts at: 1789 3828 1789
Size: 2039 2237
BlpI GC'TnA_GC
Cuts at: 1386 1386
Size: 4276
Bme1580I G_kGCm'C
Cuts at: 968 1084 1899 1992 3050 968
Size: 116 815 93 1058 2194
Fragments arranged by size:
2194 1058 815 116 93
BmrI ACTGGGnnnn_n'
Cuts at: 1098 1125 1486 1567 1902 4048 1098
Size: 27 361 81 335 2146 1326
Fragments arranged by size:
2146 1326 361 335 81 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 2641 408
Size: 2233 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 1046 462
Size: 584 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1450 2329 2823 3121 1009
Size: 441 879 494 298 2164
Fragments arranged by size:
2164 879 494 441 298
BsaI GGTCTCn'nnnn_
Cuts at: 428 3980 428
Size: 3552 724
BsaAI yAC'GTr
Cuts at: 1266 1627 1266
Size: 361 3915
BsaBI GATnn'nnATC
Cuts at: 6 755 1788 3827 6
Size: 749 1033 2039 455
Fragments arranged by size:
2039 1033 749 455
BsaHI Gr'CG_yC
Cuts at: 1168 1705 1953 2657 4153 1168
Size: 537 248 704 1496 1291
Fragments arranged by size:
1496 1291 704 537 248
BsaWI w'CCGG_w
Cuts at: 1463 1984 2664 2938 3085 3496 1463
Size: 521 680 274 147 411 2243
Fragments arranged by size:
2243 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 1006 1200 652
Size: 354 194 3728
Fragments arranged by size:
3728 354 194
BseYI C'CCAG_C
Cuts at: 629 1699 2457 3036 3532 629
Size: 1070 758 579 496 1373
Fragments arranged by size:
1373 1070 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1214 1214
Size: 4276
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3072 104
Size: 1712 46 1210 1308
Fragments arranged by size:
1712 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 252 389 1162 1288 2066 2256 3050 252
Size: 137 773 126 778 190 794 1478
Fragments arranged by size:
1478 794 778 773 190 137 126
BsmI GAATG_Cn'
Cuts at: 846 846
Size: 4276
Bsp1286I G_dGCh'C
Cuts at: 252 389 968 1084 1162 1288 1899 1992 2066
Size: 137 579 116 78 126 611 93 74
Cuts at: 2066 2256 2318 3050 252
Size: 190 62 732 1478
Fragments arranged by size:
1478 732 611 579 190 137 126 116
93 78 74 62
BspEI T'CCGG_A
Cuts at: 3496 3496
Size: 4276
BspHI T'CATG_A
Cuts at: 3452 3452
Size: 4276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 947 1186 1324 1538 1840 2221 947
Size: 239 138 214 302 381 3002
Fragments arranged by size:
3002 381 302 239 214 138
BsrBI CCG'CTC
Cuts at: 50 1043 2566 2620 4106 4271 50
Size: 993 1523 54 1486 165 55
Fragments arranged by size:
1523 1486 993 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4276
BsrFI r'CCGG_y
Cuts at: 2272 2453 2664 2272
Size: 181 211 3884
Fragments arranged by size:
3884 211 181
BssSI C'ACGA_G
Cuts at: 2545 2905 2545
Size: 360 3916
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4276
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4276
BstEII G'GTnAC_C
Cuts at: 304 1227 304
Size: 923 3353
BstYI r'GATC_y
Cuts at: 1 1011 1299 1766 1789 2124 2370 3384 3470
Size: 1010 288 467 23 335 246 1014 86
Cuts at: 3470 3482 3828 1
Size: 12 346 449
Fragments arranged by size:
1014 1010 467 449 346 335 288 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 910 910
Size: 4276
BtgI C'CryG_G
Cuts at: 998 998
Size: 4276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1076 1164 2224 2405 2568 1076
Size: 88 1060 181 163 2784
Fragments arranged by size:
2784 1060 181 163 88
BtsI GCAGTG_nn'
Cuts at: 833 898 1197 1517 833
Size: 65 299 320 3592
Fragments arranged by size:
3592 320 299 65
DraI TTT'AAA
Cuts at: 1242 1273 1242
Size: 31 4245
DrdI GACnn_nn'nnGTC
Cuts at: 1980 2840 1980
Size: 860 3416
EaeI y'GGCC_r
Cuts at: 1859 2033 2424 2451 3798 1859
Size: 174 391 27 1347 2337
Fragments arranged by size:
2337 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4276
EarI CTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2794 2940 4086 2482
Size: 312 146 1146 2672
Fragments arranged by size:
2672 1146 312 146
EcoICRI GAG'CTC
Cuts at: 387 1286 387
Size: 899 3377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 437 462 521 1046 2099 2531 3280 3548
Size: 216 25 59 525 1053 432 749 268
Cuts at: 3548 221
Size: 949
Fragments arranged by size:
1053 949 749 525 432 268 216 59
25
EcoO109I rG'GnC_Cy
Cuts at: 580 658 964 965 1413 580
Size: 78 306 1 448 3443
Fragments arranged by size:
3443 448 306 78 1
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4276
EcoRV GAT'ATC
Cuts at: 1122 1122
Size: 4276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4276
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4276
HaeII r_GCGC'y
Cuts at: 1708 1956 2980 3959 1708
Size: 248 1024 979 2025
Fragments arranged by size:
2025 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 521 553 2116 2146 2148 2178 276
Size: 32 213 32 1563 30 2 30 2374
Fragments arranged by size:
2374 1563 213 32 32 30 30 2
HincII GTy'rAC
Cuts at: 729 1313 729
Size: 584 3692
HindIII A'AGCT_T
Cuts at: 235 493 1244 235
Size: 258 751 3267
Fragments arranged by size:
3267 751 258
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4028
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4276
MluI A'CGCG_T
Cuts at: 3709 3709
Size: 4276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2947 3131 2947
Size: 184 4092
MscI TGG'CCA
Cuts at: 2035 2035
Size: 4276
MslI CAynn'nnrTG
Cuts at: 2263 2390 2263
Size: 127 4149
MspA1I CmG'CkG
Cuts at: 629 723 1385 1699 2059 3074 3319 629
Size: 94 662 314 360 1015 245 1586
Fragments arranged by size:
1586 1015 662 360 314 245 94
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4276
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4028
NcoI C'CATG_G
Cuts at: 998 998
Size: 4276
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4276
NruI TCG'CGA
Cuts at: 1183 2637 1183
Size: 1454 2822
NspI r_CATG'y
Cuts at: 877 1327 1827 2736 877
Size: 450 500 909 2417
Fragments arranged by size:
2417 909 500 450
PasI CC'CwG_GG
Cuts at: 654 654
Size: 4276
PciI A'CATG_T
Cuts at: 2732 2732
Size: 4276
PflMI CCAn_nnn'nTGG
Cuts at: 1137 1137
Size: 4276
PfoI T'CCnGG_A
Cuts at: 1014 1014
Size: 4276
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3415 3447 3415
Size: 32 4244
PspOMI G'GGCC_C
Cuts at: 964 964
Size: 4276
PspXI vC'TCGA_Gb
Cuts at: 821 821
Size: 4276
PstI C_TGCA'G
Cuts at: 1218 1321 2006 1218
Size: 103 685 3488
Fragments arranged by size:
3488 685 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4276
PvuII CAG'CTG
Cuts at: 629 723 1699 2059 629
Size: 94 976 360 2846
Fragments arranged by size:
2846 976 360 94
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4276
SacI G_AGCT'C
Cuts at: 389 1288 389
Size: 899 3377
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4276
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4066
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4276
ScaI AGT'ACT
Cuts at: 3793 3793
Size: 4276
SexAI A'CCwGG_T
Cuts at: 308 926 308
Size: 618 3658
SfcI C'TryA_G
Cuts at: 33 624 1214 1317 2002 2997 3188 33
Size: 591 590 103 685 995 191 1121
Fragments arranged by size:
1121 995 685 591 590 191 103
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4028
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4276
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4276
SmlI C'TyrA_G
Cuts at: 333 821 988 1429 2344 2838 3100 333
Size: 488 167 441 915 494 262 1509
Fragments arranged by size:
1509 915 494 488 441 262 167
SnaBI TAC'GTA
Cuts at: 1266 1266
Size: 4276
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4276
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3776
SspI AAT'ATT
Cuts at: 849 849
Size: 4276
StuI AGG'CCT
Cuts at: 206 206
Size: 4276
StyI C'CwwG_G
Cuts at: 218 583 951 998 1408 1762 218
Size: 365 368 47 410 354 2732
Fragments arranged by size:
2732 410 368 365 354 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 621 1177 1191 1857 1990 2449 2660 621
Size: 556 14 666 133 459 211 2237
Fragments arranged by size:
2237 666 556 459 211 133 14
TatI w'GTAC_w
Cuts at: 3791 3791
Size: 4276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3793 3825 3793
Size: 32 4244
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4276
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 1769 1005
Size: 764 3512
XhoI C'TCGA_G
Cuts at: 821 821
Size: 4276
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4276
XmnI GAAnn'nnTTC
Cuts at: 206 206
Size: 4276
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI AscI BaeI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NsiI
PacI PmlI PpuMI PshAI PsiI PsrI SacII SanDI
SfiI SgrAI SrfI SwaI ZraI