Restriction map for
pF1KSDA0839
(Circular) (Six-base) MAPSORT of: pF1KSDA0839.seq Check: 617 from: 1 to: 7318
pF1KSDA0839 7318 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 206 1029 206
Size: 823 6495
AccI GT'mk_AC
Cuts at: 897 4354 897
Size: 3457 3861
Acc65I G'GTAC_C
Cuts at: 1397 4332 1397
Size: 2935 4383
AclI AA'CG_TT
Cuts at: 1733 1932 7158 1733
Size: 199 5226 1893
Fragments arranged by size:
5226 1893 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 593 2806 2961 3005 3430 3664 4196 5141
Size: 267 2213 155 44 425 234 532 945
Cuts at: 5141 5573 6322 6590 326
Size: 432 749 268 1054
Fragments arranged by size:
2213 1054 945 749 532 432 425 268
267 234 155 44
AflII C'TTAA_G
Cuts at: 2164 2164
Size: 7318
AflIII A'CryG_T
Cuts at: 3267 4668 5774 6751 3267
Size: 1401 1106 977 3834
Fragments arranged by size:
3834 1401 1106 977
AgeI A'CCGG_T
Cuts at: 5706 5706
Size: 7318
AleI CACnn'nnGTG
Cuts at: 4177 4177
Size: 7318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2681 3189 2681
Size: 508 6810
AlwNI CAG_nnn'CTG
Cuts at: 1711 2829 4038 4621 6190 1711
Size: 1118 1209 583 1569 2839
Fragments arranged by size:
2839 1569 1209 1118 583
ApaLI G'TGCA_C
Cuts at: 6088 6088
Size: 7318
ApoI r'AATT_y
Cuts at: 1485 1544 2082 2701 3747 4320 6671 6927 1485
Size: 59 538 619 1046 573 2351 256 1876
Fragments arranged by size:
2351 1876 1046 619 573 538 256 59
AseI AT'TA_AT
Cuts at: 20 1867 20
Size: 1847 5471
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7318
AvaI C'yCGr_G
Cuts at: 176 401 4336 176
Size: 225 3935 3158
Fragments arranged by size:
3935 3158 225
BamHI G'GATC_C
Cuts at: 3741 4341 3741
Size: 600 6718
BanI G'GyrC_C
Cuts at: 220 1397 3489 4332 4746 4994 5029 220
Size: 1177 2092 843 414 248 35 2509
Fragments arranged by size:
2509 2092 1177 843 414 248 35
BanII G_rGCy'C
Cuts at: 3152 4330 5360 3152
Size: 1178 1030 5110
Fragments arranged by size:
5110 1178 1030
BbeI G_GCGC'C
Cuts at: 224 4750 4998 224
Size: 4526 248 2544
Fragments arranged by size:
4526 2544 248
BbsI GAAGACnn'nnnn_
Cuts at: 976 2497 3652 976
Size: 1521 1155 4642
Fragments arranged by size:
4642 1521 1155
BbvCI CC'TCA_GC
Cuts at: 214 2423 214
Size: 2209 5109
BciVI GTATCCnnnnn_n'
Cuts at: 547 1631 3495 5214 5977 7318 547
Size: 1084 1864 1719 763 1341 547
Fragments arranged by size:
1864 1719 1341 1084 763 547
BclI T'GATC_A
Cuts at: 921 960 4836 921
Size: 39 3876 3403
Fragments arranged by size:
3876 3403 39
BfrBI ATG'CAT
Cuts at: 3691 3691
Size: 7318
BglII A'GATC_T
Cuts at: 1688 3449 4831 6870 1688
Size: 1761 1382 2039 2136
Fragments arranged by size:
2136 2039 1761 1382
BlpI GC'TnA_GC
Cuts at: 2882 4428 2882
Size: 1546 5772
Bme1580I G_kGCm'C
Cuts at: 4941 5034 6092 4941
Size: 93 1058 6167
Fragments arranged by size:
6167 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 233 264 578 670 4528 4609 4944 7090 233
Size: 31 314 92 3858 81 335 2146 461
Fragments arranged by size:
3858 2146 461 335 314 92 81 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3615 3656 5683 3615
Size: 41 2027 5250
Fragments arranged by size:
5250 2027 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 504 2155 2824 151
Size: 353 1651 669 4645
Fragments arranged by size:
4645 1651 669 353
Bpu10I CC'TnA_GC
Cuts at: 214 2423 2527 214
Size: 2209 104 5005
Fragments arranged by size:
5005 2209 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2020 3085 3166 4492 5371 5865 6163 2020
Size: 1065 81 1326 879 494 298 3175
Fragments arranged by size:
3175 1326 1065 879 494 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 2548 7022 2548
Size: 4474 2844
BsaAI yAC'GTr
Cuts at: 4308 4669 4308
Size: 361 6957
BsaBI GATnn'nnATC
Cuts at: 6 4830 6869 6
Size: 4824 2039 455
Fragments arranged by size:
4824 2039 455
BsaHI Gr'CG_yC
Cuts at: 221 573 1278 4747 4995 5699 7195 221
Size: 352 705 3469 248 704 1496 344
Fragments arranged by size:
3469 1496 705 704 352 344 248
BsaWI w'CCGG_w
Cuts at: 4505 5026 5706 5980 6127 6538 4505
Size: 521 680 274 147 411 5285
Fragments arranged by size:
5285 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1812 1842 3840 3870 1812
Size: 30 1998 30 5260
Fragments arranged by size:
5260 1998 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 573 220
Size: 353 6965
BseYI C'CCAG_C
Cuts at: 3176 4741 5499 6078 6574 3176
Size: 1565 758 579 496 3920
Fragments arranged by size:
3920 1565 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2654 3812 2654
Size: 1158 6160
BsiEI CG_ry'CG
Cuts at: 104 4858 4904 6114 104
Size: 4754 46 1210 1308
Fragments arranged by size:
4754 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 531 647 1564 2297 3152 3596 3613 4046 4330
Size: 116 917 733 855 444 17 433 284
Cuts at: 4330 5108 5298 6092 531
Size: 778 190 794 1757
Fragments arranged by size:
1757 917 855 794 778 733 444 433
284 190 116 17
BsmI GAATG_Cn'
Cuts at: 2658 2658
Size: 7318
Bsp1286I G_dGCh'C
Cuts at: 531 647 1564 2297 3152 3596 3613 4046 4330
Size: 116 917 733 855 444 17 433 284
Cuts at: 4330 4941 5034 5108 5298 5360 6092 531
Size: 611 93 74 190 62 732 1757
Fragments arranged by size:
1757 917 855 733 732 611 444 433
284 190 116 93 74 62 17
BspEI T'CCGG_A
Cuts at: 6538 6538
Size: 7318
BspHI T'CATG_A
Cuts at: 3629 4008 6494 3629
Size: 379 2486 4453
Fragments arranged by size:
4453 2486 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 595 1029 4366 4580 4882 5263 206
Size: 389 434 3337 214 302 381 2261
Fragments arranged by size:
3337 2261 434 389 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2635 5608 5662 7148 7313 50
Size: 2585 2973 54 1486 165 55
Fragments arranged by size:
2973 2585 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2936 3168 3281 3416 4154 5228 2936
Size: 232 113 135 738 1074 5026
Fragments arranged by size:
5026 1074 738 232 135 113
BsrFI r'CCGG_y
Cuts at: 5314 5495 5706 5314
Size: 181 211 6926
Fragments arranged by size:
6926 211 181
BssSI C'ACGA_G
Cuts at: 5587 5947 5587
Size: 360 6958
BstAPI GCAn_nnn'nTGC
Cuts at: 838 2595 4693 838
Size: 1757 2098 3463
Fragments arranged by size:
3463 2098 1757
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7318
BstYI r'GATC_y
Cuts at: 1 209 1688 3204 3438 3449 3741 3831 4341
Size: 208 1479 1516 234 11 292 90 510
Cuts at: 4341 4808 4831 5166 5412 6426 6512 6524 6870
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6870 1
Size: 449
Fragments arranged by size:
1516 1479 1014 510 467 449 346 335
292 246 234 208 90 86 23 12
11
BtgI C'CryG_G
Cuts at: 125 1281 2581 3524 125
Size: 1156 1300 943 3919
Fragments arranged by size:
3919 1300 1156 943
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5266 5447 5610 5266
Size: 181 163 6974
Fragments arranged by size:
6974 181 163
BtsI GCAGTG_nn'
Cuts at: 1605 2872 4559 1605
Size: 1267 1687 4364
Fragments arranged by size:
4364 1687 1267
ClaI AT'CG_AT
Cuts at: 2549 2549
Size: 7318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 969 1004 3843 3878 969
Size: 35 2839 35 4409
Fragments arranged by size:
4409 2839 35 35
DraI TTT'AAA
Cuts at: 1646 1985 2967 4315 1646
Size: 339 982 1348 4649
Fragments arranged by size:
4649 1348 982 339
DraIII CAC_nnn'GTG
Cuts at: 1756 1794 4130 1756
Size: 38 2336 4944
Fragments arranged by size:
4944 2336 38
DrdI GACnn_nn'nnGTC
Cuts at: 4018 5022 5882 4018
Size: 1004 860 5454
Fragments arranged by size:
5454 1004 860
EaeI y'GGCC_r
Cuts at: 2543 3880 4901 5075 5466 5493 6840 2543
Size: 1337 1021 174 391 27 1347 3021
Fragments arranged by size:
3021 1347 1337 1021 391 174 27
EagI C'GGCC_G
Cuts at: 4901 4901
Size: 7318
EarI CTCTTCn'nnn_
Cuts at: 737 812 1759 2312 3836 3983 4298 5339 5549
Size: 75 947 553 1524 147 315 1041 210
Cuts at: 5549 737
Size: 2506
Fragments arranged by size:
2506 1524 1041 947 553 315 210 147
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5524 5836 5982 7128 5524
Size: 312 146 1146 5714
Fragments arranged by size:
5714 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3150 4328 3150
Size: 1178 6140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 326 504 593 2155 2806 2824 2961 3005
Size: 175 178 89 1562 651 18 137 44
Cuts at: 3005 3430 3664 4196 5141 5573 6322 6590 151
Size: 425 234 532 945 432 749 268 879
Fragments arranged by size:
1562 945 879 749 651 532 432 425
268 234 178 175 137 89 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 202 228 1580 202
Size: 26 1352 5940
Fragments arranged by size:
5940 1352 26
EcoO109I rG'GnC_Cy
Cuts at: 162 234 672 1437 1573 2736 4020 4161 4455
Size: 72 438 765 136 1163 1284 141 294
Cuts at: 4455 162
Size: 3025
Fragments arranged by size:
3025 1284 1163 765 438 294 141 136
72
EcoRI G'AATT_C
Cuts at: 4320 4320
Size: 7318
EcoRV GAT'ATC
Cuts at: 2678 2678
Size: 7318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 4039 5335 2179
Size: 1860 1296 4162
Fragments arranged by size:
4162 1860 1296
FspI TGC'GCA
Cuts at: 2599 2648 5097 2599
Size: 49 2449 4820
Fragments arranged by size:
4820 2449 49
FspAI rTGC'GCAy
Cuts at: 2599 2599
Size: 7318
HaeII r_GCGC'y
Cuts at: 224 4750 4998 6022 7001 224
Size: 4526 248 1024 979 541
Fragments arranged by size:
4526 1024 979 541 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1725 2554 2586 5158 5188 5190 5220 1693
Size: 32 829 32 2572 30 2 30 3791
Fragments arranged by size:
3791 2572 829 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3051 3975 4355 3051
Size: 924 380 6014
Fragments arranged by size:
6014 924 380
HindIII A'AGCT_T
Cuts at: 1904 1904
Size: 7318
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 7318
KasI G'GCGC_C
Cuts at: 220 4746 4994 220
Size: 4526 248 2544
Fragments arranged by size:
4526 2544 248
KpnI G_GTAC'C
Cuts at: 1401 4336 1401
Size: 2935 4383
MfeI C'AATT_G
Cuts at: 4184 4184
Size: 7318
MluI A'CGCG_T
Cuts at: 6751 6751
Size: 7318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 400 1159 3333 5989 6173 400
Size: 759 2174 2656 184 1545
Fragments arranged by size:
2656 2174 1545 759 184
MscI TGG'CCA
Cuts at: 2545 3882 5077 2545
Size: 1337 1195 4786
Fragments arranged by size:
4786 1337 1195
MslI CAynn'nnrTG
Cuts at: 595 929 3409 4177 5305 5432 595
Size: 334 2480 768 1128 127 2481
Fragments arranged by size:
2481 2480 1128 768 334 127
MspA1I CmG'CkG
Cuts at: 218 766 2427 2802 3796 4035 4427 4741 5101
Size: 548 1661 375 994 239 392 314 360
Cuts at: 5101 6116 6361 218
Size: 1015 245 1175
Fragments arranged by size:
1661 1175 1015 994 548 392 375 360
314 245 239
NaeI GCC'GGC
Cuts at: 5497 5497
Size: 7318
NarI GG'CG_CC
Cuts at: 221 4747 4995 221
Size: 4526 248 2544
Fragments arranged by size:
4526 2544 248
NcoI C'CATG_G
Cuts at: 125 1281 2581 125
Size: 1156 1300 4862
Fragments arranged by size:
4862 1300 1156
NdeI CA'TA_TG
Cuts at: 1294 1294
Size: 7318
NgoMIV G'CCGG_C
Cuts at: 5495 5495
Size: 7318
NruI TCG'CGA
Cuts at: 5679 5679
Size: 7318
NsiI A_TGCA'T
Cuts at: 3693 3693
Size: 7318
NspI r_CATG'y
Cuts at: 1389 2653 3166 3271 4369 4869 5778 1389
Size: 1264 513 105 1098 500 909 2929
Fragments arranged by size:
2929 1264 1098 909 513 500 105
PciI A'CATG_T
Cuts at: 3267 5774 3267
Size: 2507 4811
PflMI CCAn_nnn'nTGG
Cuts at: 1063 1169 1711 1063
Size: 106 542 6670
Fragments arranged by size:
6670 542 106
PfoI T'CCnGG_A
Cuts at: 158 158
Size: 7318
PmeI GTTT'AAAC
Cuts at: 4315 4315
Size: 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6457 6489 6457
Size: 32 7286
PpuMI rG'GwC_Cy
Cuts at: 162 234 672 1437 1573 4020 4161 162
Size: 72 438 765 136 2447 141 3319
Fragments arranged by size:
3319 2447 765 438 141 136 72
PsiI TTA'TAA
Cuts at: 884 884
Size: 7318
PstI C_TGCA'G
Cuts at: 843 1612 2879 4363 5048 843
Size: 769 1267 1484 685 3113
Fragments arranged by size:
3113 1484 1267 769 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7318
PvuII CAG'CTG
Cuts at: 766 2427 2802 3796 4035 4741 5101 766
Size: 1661 375 994 239 706 360 2983
Fragments arranged by size:
2983 1661 994 706 375 360 239
RsrII CG'GwC_CG
Cuts at: 5511 5511
Size: 7318
SacI G_AGCT'C
Cuts at: 3152 4330 3152
Size: 1178 6140
SalI G'TCGA_C
Cuts at: 4353 4353
Size: 7318
SanDI GG'GwC_CC
Cuts at: 234 234
Size: 7318
SapI GCTCTTCn'nnn_
Cuts at: 5339 5549 5339
Size: 210 7108
SbfI CC_TGCA'GG
Cuts at: 4363 4363
Size: 7318
ScaI AGT'ACT
Cuts at: 680 2779 6835 680
Size: 2099 4056 1163
Fragments arranged by size:
4056 2099 1163
SfcI C'TryA_G
Cuts at: 33 839 1043 1430 1608 2023 2875 4000 4359
Size: 806 204 387 178 415 852 1125 359
Cuts at: 4359 5044 6039 6230 33
Size: 685 995 191 1121
Fragments arranged by size:
1125 1121 995 852 806 685 415 387
359 204 191 178
SfoI GGC'GCC
Cuts at: 222 4748 4996 222
Size: 4526 248 2544
Fragments arranged by size:
4526 2544 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7318
SmaI CCC'GGG
Cuts at: 178 4338 178
Size: 4160 3158
SmlI C'TyrA_G
Cuts at: 401 1999 2164 3064 3145 4471 5386 5880 6142
Size: 1598 165 900 81 1326 915 494 262
Cuts at: 6142 401
Size: 1577
Fragments arranged by size:
1598 1577 1326 915 900 494 262 165
81
SnaBI TAC'GTA
Cuts at: 4308 4308
Size: 7318
SpeI A'CTAG_T
Cuts at: 67 4203 67
Size: 4136 3182
SphI G_CATG'C
Cuts at: 3166 4369 4869 3166
Size: 1203 500 5615
Fragments arranged by size:
5615 1203 500
SspI AAT'ATT
Cuts at: 2272 3257 2272
Size: 985 6333
StyI C'CwwG_G
Cuts at: 125 1281 1778 2581 2893 4450 4804 125
Size: 1156 497 803 312 1557 354 2639
Fragments arranged by size:
2639 1557 1156 803 497 354 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2710 3867 4096 4137 4899 5032 5491 5702 2710
Size: 1157 229 41 762 133 459 211 4326
Fragments arranged by size:
4326 1157 762 459 229 211 133 41
TatI w'GTAC_w
Cuts at: 678 2777 4132 6833 678
Size: 2099 1355 2701 1163
Fragments arranged by size:
2701 2099 1355 1163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 558 590 3840 3872 6835 6867 429
Size: 32 97 32 3250 32 2963 32 880
Fragments arranged by size:
3250 2963 880 97 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 948 5113 948
Size: 4165 3153
XbaI T'CTAG_A
Cuts at: 1273 4347 1273
Size: 3074 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1839 3549 4811 387
Size: 1452 1710 1262 2894
Fragments arranged by size:
2894 1710 1452 1262
XhoI C'TCGA_G
Cuts at: 401 401
Size: 7318
XmaI C'CCGG_G
Cuts at: 176 4336 176
Size: 4160 3158
XmnI GAAnn'nnTTC
Cuts at: 1868 3304 1868
Size: 1436 5882
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I FseI NheI NotI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI StuI SwaI ZraI