Restriction map for
pF1KSDA0841
(Circular) (Six-base) MAPSORT of: pF1KSDA0841.seq Check: 4206 from: 1 to: 5038
pF1KSDA0841 5038 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1134 1134
Size: 5038
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5038
Acc65I G'GTAC_C
Cuts at: 2052 2052
Size: 5038
AclI AA'CG_TT
Cuts at: 4878 4878
Size: 5038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1858 2861 3293 4042 4310 1081
Size: 777 1003 432 749 268 1809
Fragments arranged by size:
1809 1003 777 749 432 268
AfeI AGC'GCT
Cuts at: 1610 1610
Size: 5038
AflIII A'CryG_T
Cuts at: 1119 2388 3494 4471 1119
Size: 1269 1106 977 1686
Fragments arranged by size:
1686 1269 1106 977
AgeI A'CCGG_T
Cuts at: 3426 3426
Size: 5038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 893 1528 893
Size: 635 4403
AlwNI CAG_nnn'CTG
Cuts at: 417 573 720 807 984 1098 1149 1218 1248
Size: 156 147 87 177 114 51 69 30
Cuts at: 1248 1779 1836 1869 1959 2016 2341 3910 417
Size: 531 57 33 90 57 325 1569 1545
Fragments arranged by size:
1569 1545 531 325 177 156 147 114
90 87 69 57 57 51 33 30
ApaI G_GGCC'C
Cuts at: 204 519 714 2007 204
Size: 315 195 1293 3235
Fragments arranged by size:
3235 1293 315 195
ApaLI G'TGCA_C
Cuts at: 274 3808 274
Size: 3534 1504
ApoI r'AATT_y
Cuts at: 2040 4391 4647 2040
Size: 2351 256 2431
Fragments arranged by size:
2431 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5038
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5038
AvaI C'yCGr_G
Cuts at: 197 1377 2056 197
Size: 1180 679 3179
Fragments arranged by size:
3179 1180 679
BamHI G'GATC_C
Cuts at: 637 1129 1280 2061 637
Size: 492 151 781 3614
Fragments arranged by size:
3614 781 492 151
BanI G'GyrC_C
Cuts at: 183 590 1292 1434 2052 2466 2714 2749 183
Size: 407 702 142 618 414 248 35 2472
Fragments arranged by size:
2472 702 618 414 407 248 142 35
BanII G_rGCy'C
Cuts at: 204 504 519 671 714 814 1190 1378 1597
Size: 300 15 152 43 100 376 188 219
Cuts at: 1597 1949 2007 2050 3080 204
Size: 352 58 43 1030 2162
Fragments arranged by size:
2162 1030 376 352 300 219 188 152
100 58 43 43 15
BbeI G_GCGC'C
Cuts at: 594 1296 2470 2718 594
Size: 702 1174 248 2914
Fragments arranged by size:
2914 1174 702 248
BbsI GAAGACnn'nnnn_
Cuts at: 543 1750 543
Size: 1207 3831
BbvCI CC'TCA_GC
Cuts at: 1252 1252
Size: 5038
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 680 714 680
Size: 34 5004
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3697 5038 2934
Size: 763 1341 2934
Fragments arranged by size:
2934 1341 763
BclI T'GATC_A
Cuts at: 2556 2556
Size: 5038
BglI GCCn_nnn'nGGC
Cuts at: 1094 1974 2002 1094
Size: 880 28 4130
Fragments arranged by size:
4130 880 28
BglII A'GATC_T
Cuts at: 2551 4590 2551
Size: 2039 2999
BlpI GC'TnA_GC
Cuts at: 808 1612 2148 808
Size: 804 536 3698
Fragments arranged by size:
3698 804 536
Bme1580I G_kGCm'C
Cuts at: 188 204 278 519 714 1507 2007 2661 2754
Size: 16 74 241 195 793 500 654 93
Cuts at: 2754 3812 188
Size: 1058 1414
Fragments arranged by size:
1414 1058 793 654 500 241 195 93
74 16
BmgBI CAC'GTC
Cuts at: 472 647 852 472
Size: 175 205 4658
Fragments arranged by size:
4658 205 175
BmrI ACTGGGnnnn_n'
Cuts at: 163 708 1165 1965 2248 2329 2664 4810 163
Size: 545 457 800 283 81 335 2146 391
Fragments arranged by size:
2146 800 545 457 391 335 283 81
BmtI G_CTAG'C
Cuts at: 136 1393 136
Size: 1257 3781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 523 3403 523
Size: 2880 2158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 685 957 1075 1486 1581 1619 1867 1891
Size: 246 272 118 411 95 38 248 24
Cuts at: 1891 439
Size: 3586
Fragments arranged by size:
3586 411 272 248 246 118 95 38
24
Bpu10I CC'TnA_GC
Cuts at: 446 1252 1883 446
Size: 806 631 3601
Fragments arranged by size:
3601 806 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 1174 2212 3091 3585 3883 467
Size: 707 1038 879 494 298 1622
Fragments arranged by size:
1622 1038 879 707 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1044 1303 4742 1044
Size: 259 3439 1340
Fragments arranged by size:
3439 1340 259
BsaAI yAC'GTr
Cuts at: 2028 2389 2028
Size: 361 4677
BsaBI GATnn'nnATC
Cuts at: 6 2550 4589 6
Size: 2544 2039 455
Fragments arranged by size:
2544 2039 455
BsaHI Gr'CG_yC
Cuts at: 591 1293 2467 2715 3419 4915 591
Size: 702 1174 248 704 1496 714
Fragments arranged by size:
1496 1174 714 704 702 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3426 3700 3847 4258 2225
Size: 521 680 274 147 411 3005
Fragments arranged by size:
3005 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 499 1120 1321 1483 1957 496
Size: 3 621 201 162 474 3577
Fragments arranged by size:
3577 621 474 201 162 3
BseYI C'CCAG_C
Cuts at: 161 491 523 1043 1082 1231 1623 1724 1985
Size: 330 32 520 39 149 392 101 261
Cuts at: 1985 2461 3219 3798 4294 161
Size: 476 758 579 496 905
Fragments arranged by size:
905 758 579 520 496 476 392 330
261 149 101 39 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 930 1183 1270 1558 2040 684
Size: 246 253 87 288 482 3682
Fragments arranged by size:
3682 482 288 253 246 87
BsiEI CG_ry'CG
Cuts at: 104 170 2578 2624 3834 104
Size: 66 2408 46 1210 1308
Fragments arranged by size:
2408 1308 1210 66 46
BsiHKAI G_wGCw'C
Cuts at: 278 504 814 872 1054 1190 1949 2050 2828
Size: 226 310 58 182 136 759 101 778
Cuts at: 2828 3018 3812 278
Size: 190 794 1504
Fragments arranged by size:
1504 794 778 759 310 226 190 182
136 101 58
BsmBI CGTCTCn'nnnn_
Cuts at: 814 1110 814
Size: 296 4742
Bsp1286I G_dGCh'C
Cuts at: 188 204 278 504 519 671 714 814 872
Size: 16 74 226 15 152 43 100 58
Cuts at: 872 1054 1190 1378 1507 1597 1949 2007 2050
Size: 182 136 188 129 90 352 58 43
Cuts at: 2050 2661 2754 2828 3018 3080 3812 188
Size: 611 93 74 190 62 732 1414
Fragments arranged by size:
1414 732 611 352 226 190 188 182
152 136 129 100 93 90 74 74
62 58 58 43 43 16 15
BspEI T'CCGG_A
Cuts at: 4258 4258
Size: 5038
BspHI T'CATG_A
Cuts at: 771 4214 771
Size: 3443 1595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1134 2086 2300 2602 2983 1134
Size: 952 214 302 381 3189
Fragments arranged by size:
3189 952 381 302 214
BsrBI CCG'CTC
Cuts at: 50 885 1068 3328 3382 4868 5033 50
Size: 835 183 2260 54 1486 165 55
Fragments arranged by size:
2260 1486 835 183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2948 2948
Size: 5038
BsrFI r'CCGG_y
Cuts at: 1662 3034 3215 3426 1662
Size: 1372 181 211 3274
Fragments arranged by size:
3274 1372 211 181
BssSI C'ACGA_G
Cuts at: 3307 3667 3307
Size: 360 4678
BstAPI GCAn_nnn'nTGC
Cuts at: 273 573 1248 2413 273
Size: 300 675 1165 2898
Fragments arranged by size:
2898 1165 675 300
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5038
BstYI r'GATC_y
Cuts at: 1 299 558 637 1129 1280 1728 1787 2061
Size: 298 259 79 492 151 448 59 274
Cuts at: 2061 2528 2551 2886 3132 4146 4232 4244 4590
Size: 467 23 335 246 1014 86 12 346
Cuts at: 4590 1
Size: 449
Fragments arranged by size:
1014 492 467 449 448 346 335 298
274 259 246 151 86 79 59 23
12
BtgI C'CryG_G
Cuts at: 125 188 458 671 992 1547 125
Size: 63 270 213 321 555 3616
Fragments arranged by size:
3616 555 321 270 213 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2986 3167 3330 2986
Size: 181 163 4694
Fragments arranged by size:
4694 181 163
BtsI GCAGTG_nn'
Cuts at: 808 918 1436 2279 808
Size: 110 518 843 3567
Fragments arranged by size:
3567 843 518 110
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5038
DrdI GACnn_nn'nnGTC
Cuts at: 987 2742 3602 987
Size: 1755 860 2423
Fragments arranged by size:
2423 1755 860
EaeI y'GGCC_r
Cuts at: 324 455 1349 2621 2795 3186 3213 4560 324
Size: 131 894 1272 174 391 27 1347 802
Fragments arranged by size:
1347 1272 894 802 391 174 131 27
EagI C'GGCC_G
Cuts at: 2621 2621
Size: 5038
EarI CTCTTCn'nnn_
Cuts at: 167 1682 1834 3059 3269 167
Size: 1515 152 1225 210 1936
Fragments arranged by size:
1936 1515 1225 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1035 1315 3244 3556 3702 4848 1035
Size: 280 1929 312 146 1146 1225
Fragments arranged by size:
1929 1225 1146 312 280 146
EcoICRI GAG'CTC
Cuts at: 502 812 1188 1947 2048 502
Size: 310 376 759 101 3492
Fragments arranged by size:
3492 759 376 310 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 685 957 1075 1081 1486 1581 1619 1858
Size: 246 272 118 6 405 95 38 239
Cuts at: 1858 1867 1891 2861 3293 4042 4310 439
Size: 9 24 970 432 749 268 1167
Fragments arranged by size:
1167 970 749 432 405 272 268 246
239 118 95 38 24 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1060 1102 1828 1939 1060
Size: 42 726 111 4159
Fragments arranged by size:
4159 726 111 42
EcoO109I rG'GnC_Cy
Cuts at: 201 954 1095 1194 1686 1956 2003 2004 2175
Size: 753 141 99 492 270 47 1 171
Cuts at: 2175 201
Size: 3064
Fragments arranged by size:
3064 753 492 270 171 141 99 47
1
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5038
EcoRV GAT'ATC
Cuts at: 941 941
Size: 5038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 5038
FspI TGC'GCA
Cuts at: 220 2817 220
Size: 2597 2441
HaeII r_GCGC'y
Cuts at: 594 1089 1170 1296 1612 2470 2718 3742 4721
Size: 495 81 126 316 858 248 1024 979
Cuts at: 4721 594
Size: 911
Fragments arranged by size:
1024 979 911 858 495 316 248 126
81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 886 918 2878 2908 2910 2940 886
Size: 32 1960 30 2 30 2984
Fragments arranged by size:
2984 1960 32 30 30 2
HincII GTy'rAC
Cuts at: 2075 2075
Size: 5038
KasI G'GCGC_C
Cuts at: 590 1292 2466 2714 590
Size: 702 1174 248 2914
Fragments arranged by size:
2914 1174 702 248
KpnI G_GTAC'C
Cuts at: 2056 2056
Size: 5038
MluI A'CGCG_T
Cuts at: 1119 4471 1119
Size: 3352 1686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 331 3709 3893 331
Size: 3378 184 1476
Fragments arranged by size:
3378 1476 184
MscI TGG'CCA
Cuts at: 326 2797 326
Size: 2471 2567
MslI CAynn'nnrTG
Cuts at: 457 3025 3152 457
Size: 2568 127 2343
Fragments arranged by size:
2568 2343 127
MspA1I CmG'CkG
Cuts at: 570 1047 1231 1278 1299 1572 1710 2147 2461
Size: 477 184 47 21 273 138 437 314
Cuts at: 2461 2821 3836 4081 570
Size: 360 1015 245 1527
Fragments arranged by size:
1527 1015 477 437 360 314 273 245
184 138 47 21
NaeI GCC'GGC
Cuts at: 1664 3217 1664
Size: 1553 3485
NarI GG'CG_CC
Cuts at: 591 1293 2467 2715 591
Size: 702 1174 248 2914
Fragments arranged by size:
2914 1174 702 248
NcoI C'CATG_G
Cuts at: 125 458 992 125
Size: 333 534 4171
Fragments arranged by size:
4171 534 333
NgoMIV G'CCGG_C
Cuts at: 1662 3215 1662
Size: 1553 3485
NheI G'CTAG_C
Cuts at: 132 1389 132
Size: 1257 3781
NruI TCG'CGA
Cuts at: 3399 3399
Size: 5038
NspI r_CATG'y
Cuts at: 274 2089 2589 3498 274
Size: 1815 500 909 1814
Fragments arranged by size:
1815 1814 909 500
PasI CC'CwG_GG
Cuts at: 1998 1998
Size: 5038
PciI A'CATG_T
Cuts at: 3494 3494
Size: 5038
PflMI CCAn_nnn'nTGG
Cuts at: 417 855 1098 1302 1623 417
Size: 438 243 204 321 3832
Fragments arranged by size:
3832 438 321 243 204
PfoI T'CCnGG_A
Cuts at: 398 1007 398
Size: 609 4429
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4177 4209 4177
Size: 32 5006
PpuMI rG'GwC_Cy
Cuts at: 1686 1686
Size: 5038
PspOMI G'GGCC_C
Cuts at: 200 515 710 2003 200
Size: 315 195 1293 3235
Fragments arranged by size:
3235 1293 315 195
PstI C_TGCA'G
Cuts at: 578 599 725 882 1223 1703 1787 1892 2012
Size: 21 126 157 341 480 84 105 120
Cuts at: 2012 2083 2768 578
Size: 71 685 2848
Fragments arranged by size:
2848 685 480 341 157 126 120 105
84 71 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5038
PvuII CAG'CTG
Cuts at: 1231 1299 1572 2461 2821 1231
Size: 68 273 889 360 3448
Fragments arranged by size:
3448 889 360 273 68
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5038
SacI G_AGCT'C
Cuts at: 504 814 1190 1949 2050 504
Size: 310 376 759 101 3492
Fragments arranged by size:
3492 759 376 310 101
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5038
SapI GCTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4828
SbfI CC_TGCA'GG
Cuts at: 599 1703 2083 599
Size: 1104 380 3554
Fragments arranged by size:
3554 1104 380
ScaI AGT'ACT
Cuts at: 4555 4555
Size: 5038
SfcI C'TryA_G
Cuts at: 33 574 595 721 878 1219 1261 1699 1783
Size: 541 21 126 157 341 42 438 84
Cuts at: 1783 1888 2008 2079 2764 3759 3950 33
Size: 105 120 71 685 995 191 1121
Fragments arranged by size:
1121 995 685 541 438 341 191 157
126 120 105 84 71 42 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2002 2002
Size: 5038
SfoI GGC'GCC
Cuts at: 592 1294 2468 2716 592
Size: 702 1174 248 2914
Fragments arranged by size:
2914 1174 702 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5038
SmaI CCC'GGG
Cuts at: 199 1379 2058 199
Size: 1180 679 3179
Fragments arranged by size:
3179 1180 679
SmlI C'TyrA_G
Cuts at: 482 1189 2191 3106 3600 3862 482
Size: 707 1002 915 494 262 1658
Fragments arranged by size:
1658 1002 915 707 494 262
SnaBI TAC'GTA
Cuts at: 2028 2028
Size: 5038
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5038
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4538
SrfI GCCC'GGGC
Cuts at: 199 199
Size: 5038
StuI AGG'CCT
Cuts at: 1645 1763 1645
Size: 118 4920
StyI C'CwwG_G
Cuts at: 125 458 863 992 1588 2170 2524 125
Size: 333 405 129 596 582 354 2639
Fragments arranged by size:
2639 596 582 405 354 333 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 890 1018 1786 2619 2752 3211 3422 505
Size: 385 128 768 833 133 459 211 2121
Fragments arranged by size:
2121 833 768 459 385 211 133 128
TatI w'GTAC_w
Cuts at: 4553 4553
Size: 5038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1014 1046 1567 1599 4555 4587 1014
Size: 32 521 32 2956 32 1465
Fragments arranged by size:
2956 1465 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2833 2833
Size: 5038
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5038
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 2531
Size: 5038
XmaI C'CCGG_G
Cuts at: 197 1377 2056 197
Size: 1180 679 3179
Fragments arranged by size:
3179 1180 679
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BfrBI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NotI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SspI
SwaI XhoI XmnI ZraI