Restriction map for
pF1KSDA0842
(Circular) (Six-base) MAPSORT of: pF1KSDA0842.seq Check: 3287 from: 1 to: 6196
pF1KSDA0842 6196 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1910 1910
Size: 6196
AatII G_ACGT'C
Cuts at: 2564 2564
Size: 6196
AccI GT'mk_AC
Cuts at: 1889 2030 3232 1889
Size: 141 1202 4853
Fragments arranged by size:
4853 1202 141
Acc65I G'GTAC_C
Cuts at: 3210 3210
Size: 6196
AclI AA'CG_TT
Cuts at: 6036 6036
Size: 6196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4019 4451 5200 5468 4019
Size: 432 749 268 4747
Fragments arranged by size:
4747 749 432 268
AfeI AGC'GCT
Cuts at: 1288 1339 1818 1288
Size: 51 479 5666
Fragments arranged by size:
5666 479 51
AflIII A'CryG_T
Cuts at: 1922 3546 4652 5629 1922
Size: 1624 1106 977 2489
Fragments arranged by size:
2489 1624 1106 977
AgeI A'CCGG_T
Cuts at: 4584 4584
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 699 2106 699
Size: 1407 4789
AleI CACnn'nnGTG
Cuts at: 985 985
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 258 648 961 1026 1353 1400 1704 1756 2001
Size: 390 313 65 327 47 304 52 245
Cuts at: 2001 2169 2780 3128 3149 3499 5068 258
Size: 168 611 348 21 350 1569 1386
Fragments arranged by size:
1569 1386 611 390 350 348 327 313
304 245 168 65 52 47 21
ApaI G_GGCC'C
Cuts at: 2404 2404
Size: 6196
ApaLI G'TGCA_C
Cuts at: 988 2145 2368 4966 988
Size: 1157 223 2598 2218
Fragments arranged by size:
2598 2218 1157 223
ApoI r'AATT_y
Cuts at: 2305 3094 3198 5549 5805 2305
Size: 789 104 2351 256 2696
Fragments arranged by size:
2696 2351 789 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 6196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6196
AvaI C'yCGr_G
Cuts at: 1054 1450 2428 3167 3214 1054
Size: 396 978 739 47 4036
Fragments arranged by size:
4036 978 739 396 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 628 661 628
Size: 33 6163
BamHI G'GATC_C
Cuts at: 152 2531 3219 152
Size: 2379 688 3129
Fragments arranged by size:
3129 2379 688
BanI G'GyrC_C
Cuts at: 2434 2473 3210 3624 3872 3907 2434
Size: 39 737 414 248 35 4723
Fragments arranged by size:
4723 737 414 248 39 35
BanII G_rGCy'C
Cuts at: 919 1055 1421 1855 2404 3208 4238 919
Size: 136 366 434 549 804 1030 2877
Fragments arranged by size:
2877 1030 804 549 434 366 136
BbeI G_GCGC'C
Cuts at: 3628 3876 3628
Size: 248 5948
BbsI GAAGACnn'nnnn_
Cuts at: 668 2509 668
Size: 1841 4355
BbvCI CC'TCA_GC
Cuts at: 1820 1820
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 322 356 1035 1069 1347 1381 322
Size: 34 679 34 278 34 5137
Fragments arranged by size:
5137 679 278 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1674 4092 4855 6196 1674
Size: 2418 763 1341 1674
Fragments arranged by size:
2418 1674 1341 763
BclI T'GATC_A
Cuts at: 2231 3714 2231
Size: 1483 4713
BglI GCCn_nnn'nGGC
Cuts at: 1190 2707 1190
Size: 1517 4679
BglII A'GATC_T
Cuts at: 3709 5748 3709
Size: 2039 4157
BlpI GC'TnA_GC
Cuts at: 1415 2521 3306 1415
Size: 1106 785 4305
Fragments arranged by size:
4305 1106 785
Bme1580I G_kGCm'C
Cuts at: 992 1979 2149 2372 2404 2437 2476 2869 3819
Size: 987 170 223 32 33 39 393 950
Cuts at: 3819 3912 4970 992
Size: 93 1058 2218
Fragments arranged by size:
2218 1058 987 950 393 223 170 93
39 33 32
BmgBI CAC'GTC
Cuts at: 2507 2507
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 327 840 2382 3406 3487 3822 5968 327
Size: 513 1542 1024 81 335 2146 555
Fragments arranged by size:
2146 1542 1024 555 513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 183 1712 4561 183
Size: 1529 2849 1818
Fragments arranged by size:
2849 1818 1529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 760 1726 2698 178
Size: 582 966 972 3676
Fragments arranged by size:
3676 972 966 582
Bpu10I CC'TnA_GC
Cuts at: 1354 1697 1820 2980 1354
Size: 343 123 1160 4570
Fragments arranged by size:
4570 1160 343 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 3129 3370 4249 4743 5041 1768
Size: 1361 241 879 494 298 2923
Fragments arranged by size:
2923 1361 879 494 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 836 1784 3164 5900 836
Size: 948 1380 2736 1132
Fragments arranged by size:
2736 1380 1132 948
BsaAI yAC'GTr
Cuts at: 381 1923 2007 2825 3186 3547 381
Size: 1542 84 818 361 361 3030
Fragments arranged by size:
3030 1542 818 361 361 84
BsaBI GATnn'nnATC
Cuts at: 6 3708 5747 6
Size: 3702 2039 455
Fragments arranged by size:
3702 2039 455
BsaHI Gr'CG_yC
Cuts at: 1663 2561 3625 3873 4577 6073 1663
Size: 898 1064 248 704 1496 1786
Fragments arranged by size:
1786 1496 1064 898 704 248
BsaWI w'CCGG_w
Cuts at: 149 230 1961 2039 3383 3904 4584 4858 5005
Size: 81 1731 78 1344 521 680 274 147
Cuts at: 5005 5416 149
Size: 411 929
Fragments arranged by size:
1731 1344 929 680 521 411 274 147
81 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 1431 1461 3129 3159 421
Size: 30 980 30 1668 30 3458
Fragments arranged by size:
3458 1668 980 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 297 960 961 1027 1132 1261 1658 1661 1664
Size: 663 1 66 105 129 397 3 3
Cuts at: 1664 2947 297
Size: 1283 3546
Fragments arranged by size:
3546 1283 663 397 129 105 66 3
3 1
BseYI C'CCAG_C
Cuts at: 673 820 923 1393 1712 1798 2260 2773 2877
Size: 147 103 470 319 86 462 513 104
Cuts at: 2877 3619 4377 4956 5452 673
Size: 742 758 579 496 1417
Fragments arranged by size:
1417 758 742 579 513 496 470 462
319 147 104 103 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 2443 2970 2999 1029
Size: 1414 527 29 4226
Fragments arranged by size:
4226 1414 527 29
BsiEI CG_ry'CG
Cuts at: 104 2667 3159 3736 3782 4992 104
Size: 2563 492 577 46 1210 1308
Fragments arranged by size:
2563 1308 1210 577 492 46
BsiHKAI G_wGCw'C
Cuts at: 269 326 992 1055 1408 1972 2149 2372 2522
Size: 57 666 63 353 564 177 223 150
Cuts at: 2522 3208 3986 4176 4970 269
Size: 686 778 190 794 1495
Fragments arranged by size:
1495 794 778 686 666 564 353 223
190 177 150 63 57
BsmI GAATG_Cn'
Cuts at: 510 510
Size: 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2901 2901
Size: 6196
Bsp1286I G_dGCh'C
Cuts at: 269 326 919 992 1055 1408 1421 1855 1972
Size: 57 593 73 63 353 13 434 117
Cuts at: 1972 1979 2149 2372 2404 2437 2476 2522 2869
Size: 7 170 223 32 33 39 46 347
Cuts at: 2869 3208 3819 3912 3986 4176 4238 4970 269
Size: 339 611 93 74 190 62 732 1495
Fragments arranged by size:
1495 732 611 593 434 353 347 339
223 190 170 117 93 74 73 63
62 57 46 39 33 32 13 7
BspEI T'CCGG_A
Cuts at: 230 1961 2039 5416 230
Size: 1731 78 3377 1010
Fragments arranged by size:
3377 1731 1010 78
BspHI T'CATG_A
Cuts at: 1913 5372 1913
Size: 3459 2737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 656 1910 2000 2042 3244 3458 3760 4141
Size: 195 1254 90 42 1202 214 302 381
Cuts at: 4141 461
Size: 2516
Fragments arranged by size:
2516 1254 1202 381 302 214 195 90
42
BsrBI CCG'CTC
Cuts at: 50 872 2858 4486 4540 6026 6191 50
Size: 822 1986 1628 54 1486 165 55
Fragments arranged by size:
1986 1628 1486 822 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4106 4106
Size: 6196
BsrFI r'CCGG_y
Cuts at: 951 1441 2666 4192 4373 4584 951
Size: 490 1225 1526 181 211 2563
Fragments arranged by size:
2563 1526 1225 490 211 181
BssSI C'ACGA_G
Cuts at: 325 1686 4465 4825 325
Size: 1361 2779 360 1696
Fragments arranged by size:
2779 1696 1361 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1403 2169 2789 3571 1403
Size: 766 620 782 4028
Fragments arranged by size:
4028 782 766 620
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1068 1068
Size: 6196
BstXI CCAn_nnnn'nTGG
Cuts at: 393 738 2310 393
Size: 345 1572 4279
Fragments arranged by size:
4279 1572 345
BstYI r'GATC_y
Cuts at: 1 152 218 585 2531 3219 3686 3709 4044
Size: 151 66 367 1946 688 467 23 335
Cuts at: 4044 4290 5304 5390 5402 5748 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1946 1014 688 467 449 367 346 335
246 151 86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 1616 1616
Size: 6196
BtgI C'CryG_G
Cuts at: 125 684 1271 1331 2282 2627 3153 125
Size: 559 587 60 951 345 526 3168
Fragments arranged by size:
3168 951 587 559 526 345 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4144 4325 4488 4144
Size: 181 163 5852
Fragments arranged by size:
5852 181 163
BtsI GCAGTG_nn'
Cuts at: 812 2415 2605 3437 812
Size: 1603 190 832 3571
Fragments arranged by size:
3571 1603 832 190
DraI TTT'AAA
Cuts at: 3193 3193
Size: 6196
DraIII CAC_nnn'GTG
Cuts at: 1403 1403
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 552 2026 3900 4760 552
Size: 1474 1874 860 1988
Fragments arranged by size:
1988 1874 1474 860
EaeI y'GGCC_r
Cuts at: 383 1563 1587 1626 2300 2708 3156 3779 3953
Size: 1180 24 39 674 408 448 623 174
Cuts at: 3953 4344 4371 5718 383
Size: 391 27 1347 861
Fragments arranged by size:
1347 1180 861 674 623 448 408 391
174 39 27 24
EagI C'GGCC_G
Cuts at: 3156 3779 3156
Size: 623 5573
EarI CTCTTCn'nnn_
Cuts at: 544 1631 1934 2069 4217 4427 544
Size: 1087 303 135 2148 210 2313
Fragments arranged by size:
2313 2148 1087 303 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4402 4714 4860 6006 4402
Size: 312 146 1146 4592
Fragments arranged by size:
4592 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1053 3206 1053
Size: 2153 4043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 760 1726 2698 4019 4451 5200 5468 178
Size: 582 966 972 1321 432 749 268 906
Fragments arranged by size:
1321 972 966 906 749 582 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 909 1357 2488 909
Size: 448 1131 4617
Fragments arranged by size:
4617 1131 448
EcoO109I rG'GnC_Cy
Cuts at: 246 1023 1735 1760 2379 2401 3333 246
Size: 777 712 25 619 22 932 3109
Fragments arranged by size:
3109 932 777 712 619 25 22
EcoRI G'AATT_C
Cuts at: 3198 3198
Size: 6196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 685 2526 4213 685
Size: 1841 1687 2668
Fragments arranged by size:
2668 1841 1687
FspI TGC'GCA
Cuts at: 3975 3975
Size: 6196
HaeII r_GCGC'y
Cuts at: 930 1290 1341 1820 2194 2894 3128 3628 3876
Size: 360 51 479 374 700 234 500 248
Cuts at: 3876 4900 5879 930
Size: 1024 979 1247
Fragments arranged by size:
1247 1024 979 700 500 479 374 360
248 234 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 338 370 685 717 949 981 1431
Size: 32 14 32 315 32 232 32 450
Cuts at: 1431 1463 2092 2124 2709 2741 3129 3161 4036
Size: 32 629 32 585 32 388 32 875
Cuts at: 4036 4066 4068 4098 292
Size: 30 2 30 2390
Fragments arranged by size:
2390 875 629 585 450 388 315 232
32 32 32 32 32 32 32 32
30 30 14 2
HincII GTy'rAC
Cuts at: 3233 3233
Size: 6196
KasI G'GCGC_C
Cuts at: 3624 3872 3624
Size: 248 5948
KpnI G_GTAC'C
Cuts at: 3214 3214
Size: 6196
MluI A'CGCG_T
Cuts at: 5629 5629
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 908 2687 3107 4867 5051 908
Size: 1779 420 1760 184 2053
Fragments arranged by size:
2053 1779 1760 420 184
MscI TGG'CCA
Cuts at: 385 1589 1628 2302 3955 385
Size: 1204 39 674 1653 2626
Fragments arranged by size:
2626 1653 1204 674 39
MslI CAynn'nnrTG
Cuts at: 985 1330 4183 4310 985
Size: 345 2853 127 2871
Fragments arranged by size:
2871 2853 345 127
MspA1I CmG'CkG
Cuts at: 1386 1596 1716 1947 1998 3135 3155 3305 3619
Size: 210 120 231 51 1137 20 150 314
Cuts at: 3619 3979 4994 5239 1386
Size: 360 1015 245 2343
Fragments arranged by size:
2343 1137 1015 360 314 245 231 210
150 120 51 20
NaeI GCC'GGC
Cuts at: 953 4375 953
Size: 3422 2774
NarI GG'CG_CC
Cuts at: 3625 3873 3625
Size: 248 5948
NcoI C'CATG_G
Cuts at: 125 1331 2282 125
Size: 1206 951 4039
Fragments arranged by size:
4039 1206 951
NgoMIV G'CCGG_C
Cuts at: 951 4373 951
Size: 3422 2774
NruI TCG'CGA
Cuts at: 4557 4557
Size: 6196
NspI r_CATG'y
Cuts at: 209 624 1603 2457 3247 3747 4656 209
Size: 415 979 854 790 500 909 1749
Fragments arranged by size:
1749 979 909 854 790 500 415
PasI CC'CwG_GG
Cuts at: 2397 2760 2966 2397
Size: 363 206 5627
Fragments arranged by size:
5627 363 206
PciI A'CATG_T
Cuts at: 4652 4652
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1026 1204 1400 1756 1026
Size: 178 196 356 5466
Fragments arranged by size:
5466 356 196 178
PfoI T'CCnGG_A
Cuts at: 155 464 3071 155
Size: 309 2607 3280
Fragments arranged by size:
3280 2607 309
PmeI GTTT'AAAC
Cuts at: 3193 3193
Size: 6196
PmlI CAC'GTG
Cuts at: 381 1923 381
Size: 1542 4654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 1965 5335 5367 1933
Size: 32 3370 32 2762
Fragments arranged by size:
3370 2762 32 32
PpuMI rG'GwC_Cy
Cuts at: 246 1760 2379 246
Size: 1514 619 4063
Fragments arranged by size:
4063 1514 619
PspOMI G'GGCC_C
Cuts at: 2400 2400
Size: 6196
PspXI vC'TCGA_Gb
Cuts at: 1054 1054
Size: 6196
PstI C_TGCA'G
Cuts at: 377 520 1147 1581 1997 3241 3926 377
Size: 143 627 434 416 1244 685 2647
Fragments arranged by size:
2647 1244 685 627 434 416 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6196
PvuII CAG'CTG
Cuts at: 1386 1716 1947 1998 3619 3979 1386
Size: 330 231 51 1621 360 3603
Fragments arranged by size:
3603 1621 360 330 231 51
RsrII CG'GwC_CG
Cuts at: 1439 2550 4389 1439
Size: 1111 1839 3246
Fragments arranged by size:
3246 1839 1111
SacI G_AGCT'C
Cuts at: 1055 3208 1055
Size: 2153 4043
SacII CC_GC'GG
Cuts at: 3156 3156
Size: 6196
SalI G'TCGA_C
Cuts at: 3231 3231
Size: 6196
SanDI GG'GwC_CC
Cuts at: 1760 1760
Size: 6196
SapI GCTCTTCn'nnn_
Cuts at: 544 1934 4217 4427 544
Size: 1390 2283 210 2313
Fragments arranged by size:
2313 2283 1390 210
SbfI CC_TGCA'GG
Cuts at: 1581 3241 1581
Size: 1660 4536
ScaI AGT'ACT
Cuts at: 493 2920 5713 493
Size: 2427 2793 976
Fragments arranged by size:
2793 2427 976
SexAI A'CCwGG_T
Cuts at: 1399 2066 1399
Size: 667 5529
SfcI C'TryA_G
Cuts at: 33 250 373 516 1143 1577 1993 3237 3922
Size: 217 123 143 627 434 416 1244 685
Cuts at: 3922 4917 5108 33
Size: 995 191 1121
Fragments arranged by size:
1244 1121 995 685 627 434 416 217
191 143 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 2707 2707
Size: 6196
SfoI GGC'GCC
Cuts at: 3626 3874 3626
Size: 248 5948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6196
SmaI CCC'GGG
Cuts at: 1452 3216 1452
Size: 1764 4432
SmlI C'TyrA_G
Cuts at: 1054 1783 3108 3349 4264 4758 5020 1054
Size: 729 1325 241 915 494 262 2230
Fragments arranged by size:
2230 1325 915 729 494 262 241
SnaBI TAC'GTA
Cuts at: 3186 3186
Size: 6196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6196
SphI G_CATG'C
Cuts at: 2457 3247 3747 2457
Size: 790 500 4906
Fragments arranged by size:
4906 790 500
StuI AGG'CCT
Cuts at: 904 904
Size: 6196
StyI C'CwwG_G
Cuts at: 125 440 548 1203 1331 2282 3328 3682 125
Size: 315 108 655 128 951 1046 354 2639
Fragments arranged by size:
2639 1046 951 655 354 315 128 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 309 669 937 3017 3777 3910 4369 4580 309
Size: 360 268 2080 760 133 459 211 1925
Fragments arranged by size:
2080 1925 760 459 360 268 211 133
TatI w'GTAC_w
Cuts at: 491 2918 5711 491
Size: 2427 2793 976
Fragments arranged by size:
2793 2427 976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5713 5745 5713
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 244 856 963 3991 244
Size: 612 107 3028 2449
Fragments arranged by size:
3028 2449 612 107
XbaI T'CTAG_A
Cuts at: 3225 3225
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1338 2289 3045 3689 1338
Size: 951 756 644 3845
Fragments arranged by size:
3845 951 756 644
XhoI C'TCGA_G
Cuts at: 1054 1054
Size: 6196
XmaI C'CCGG_G
Cuts at: 1450 3214 1450
Size: 1764 4432
XmnI GAAnn'nnTTC
Cuts at: 937 3094 937
Size: 2157 4039
ZraI GAC'GTC
Cuts at: 2562 2562
Size: 6196
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AloI AscI AvrII BfrBI BmtI BsiWI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PshAI PsiI PsrI SgrAI SrfI SspI SwaI