Restriction map for
pF1KSDA0849
(Circular) (Six-base) MAPSORT of: pF1KSDA0849.seq Check: 2293 from: 1 to: 5998
pF1KSDA0849 5998 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 3034 3034
Size: 5998
Acc65I G'GTAC_C
Cuts at: 3012 3012
Size: 5998
AclI AA'CG_TT
Cuts at: 5838 5838
Size: 5998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 1565 1653 1720 1744 2168 3821 4253 5002
Size: 1013 88 67 24 424 1653 432 749
Cuts at: 5002 5270 552
Size: 268 1280
Fragments arranged by size:
1653 1280 1013 749 432 424 268 88
67 24
AfeI AGC'GCT
Cuts at: 1786 1786
Size: 5998
AflII C'TTAA_G
Cuts at: 1815 1815
Size: 5998
AflIII A'CryG_T
Cuts at: 3348 4454 5431 3348
Size: 1106 977 3915
Fragments arranged by size:
3915 1106 977
AgeI A'CCGG_T
Cuts at: 4386 4386
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 377 2964 377
Size: 2587 3411
AlwNI CAG_nnn'CTG
Cuts at: 781 1153 3301 4870 781
Size: 372 2148 1569 1909
Fragments arranged by size:
2148 1909 1569 372
ApaLI G'TGCA_C
Cuts at: 2918 4768 2918
Size: 1850 4148
ApoI r'AATT_y
Cuts at: 344 547 2167 2264 2347 2420 3000 5351 5607
Size: 203 1620 97 83 73 580 2351 256
Cuts at: 5607 344
Size: 735
Fragments arranged by size:
2351 1620 735 580 256 203 97 83
73
AseI AT'TA_AT
Cuts at: 20 20
Size: 5998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5998
AvaI C'yCGr_G
Cuts at: 3016 3016
Size: 5998
BamHI G'GATC_C
Cuts at: 3021 3021
Size: 5998
BanI G'GyrC_C
Cuts at: 2358 3012 3426 3674 3709 2358
Size: 654 414 248 35 4647
Fragments arranged by size:
4647 654 414 248 35
BanII G_rGCy'C
Cuts at: 1537 3010 4040 1537
Size: 1473 1030 3495
Fragments arranged by size:
3495 1473 1030
BbeI G_GCGC'C
Cuts at: 3430 3678 3430
Size: 248 5750
BbsI GAAGACnn'nnnn_
Cuts at: 1190 1268 2468 2885 2931 1190
Size: 78 1200 417 46 4257
Fragments arranged by size:
4257 1200 417 78 46
BciVI GTATCCnnnnn_n'
Cuts at: 3894 4657 5998 3894
Size: 763 1341 3894
Fragments arranged by size:
3894 1341 763
BclI T'GATC_A
Cuts at: 3516 3516
Size: 5998
BfrBI ATG'CAT
Cuts at: 2941 2941
Size: 5998
BglII A'GATC_T
Cuts at: 532 1168 3511 5550 532
Size: 636 2343 2039 980
Fragments arranged by size:
2343 2039 980 636
BlpI GC'TnA_GC
Cuts at: 3108 3108
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1698 2922 3621 3714 4772 1698
Size: 1224 699 93 1058 2924
Fragments arranged by size:
2924 1224 1058 699 93
BmgBI CAC'GTC
Cuts at: 504 504
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 180 960 1437 2620 2664 3208 3289 3624 5770
Size: 780 477 1183 44 544 81 335 2146
Cuts at: 5770 180
Size: 408
Fragments arranged by size:
2146 1183 780 544 477 408 335 81
44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4363 4363
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 810 1306 2682 2883 575
Size: 235 496 1376 201 3690
Fragments arranged by size:
3690 1376 496 235 201
Bpu10I CC'TnA_GC
Cuts at: 906 906
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2813 3172 4051 4545 4843 2567
Size: 246 359 879 494 298 3722
Fragments arranged by size:
3722 879 494 359 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 5702 5702
Size: 5998
BsaAI yAC'GTr
Cuts at: 2988 3349 2988
Size: 361 5637
BsaBI GATnn'nnATC
Cuts at: 6 3510 5549 6
Size: 3504 2039 455
Fragments arranged by size:
3504 2039 455
BsaHI Gr'CG_yC
Cuts at: 3427 3675 4379 5875 3427
Size: 248 704 1496 3550
Fragments arranged by size:
3550 1496 704 248
BsaWI w'CCGG_w
Cuts at: 601 2532 3185 3706 4386 4660 4807 5218 601
Size: 1931 653 521 680 274 147 411 1381
Fragments arranged by size:
1931 1381 680 653 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 221 251 1304 1334 221
Size: 30 1053 30 4885
Fragments arranged by size:
4885 1053 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 1319 460
Size: 859 5139
BseYI C'CCAG_C
Cuts at: 3421 4179 4758 5254 3421
Size: 758 579 496 4165
Fragments arranged by size:
4165 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1454 1667 994
Size: 460 213 5325
Fragments arranged by size:
5325 460 213
BsiEI CG_ry'CG
Cuts at: 104 2796 3538 3584 4794 104
Size: 2692 742 46 1210 1308
Fragments arranged by size:
2692 1308 1210 742 46
BsiHKAI G_wGCw'C
Cuts at: 1619 2922 3010 3788 3978 4772 1619
Size: 1303 88 778 190 794 2845
Fragments arranged by size:
2845 1303 794 778 190 88
BsmI GAATG_Cn'
Cuts at: 211 294 2524 2886 211
Size: 83 2230 362 3323
Fragments arranged by size:
3323 2230 362 83
Bsp1286I G_dGCh'C
Cuts at: 1537 1619 1698 2922 3010 3621 3714 3788 3978
Size: 82 79 1224 88 611 93 74 190
Cuts at: 3978 4040 4772 1537
Size: 62 732 2763
Fragments arranged by size:
2763 1224 732 611 190 93 88 82
79 74 62
BspEI T'CCGG_A
Cuts at: 601 2532 5218 601
Size: 1931 2686 1381
Fragments arranged by size:
2686 1931 1381
BspHI T'CATG_A
Cuts at: 5174 5174
Size: 5998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 786 2221 2581 2891 3046 3260 3562 3943
Size: 20 1435 360 310 155 214 302 381
Cuts at: 3943 766
Size: 2821
Fragments arranged by size:
2821 1435 381 360 310 302 214 155
20
BsrBI CCG'CTC
Cuts at: 50 180 4288 4342 5828 5993 50
Size: 130 4108 54 1486 165 55
Fragments arranged by size:
4108 1486 165 130 55 54
BsrDI GCAATG_nn'
Cuts at: 1756 2507 3908 1756
Size: 751 1401 3846
Fragments arranged by size:
3846 1401 751
BsrFI r'CCGG_y
Cuts at: 1764 3994 4175 4386 1764
Size: 2230 181 211 3376
Fragments arranged by size:
3376 2230 211 181
BsrGI T'GTAC_A
Cuts at: 2978 2978
Size: 5998
BssSI C'ACGA_G
Cuts at: 4267 4627 4267
Size: 360 5638
BstAPI GCAn_nnn'nTGC
Cuts at: 2681 3373 2681
Size: 692 5306
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5998
BstYI r'GATC_y
Cuts at: 1 532 1138 1168 2157 3021 3488 3511 3846
Size: 531 606 30 989 864 467 23 335
Cuts at: 3846 4092 5106 5192 5204 5550 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 989 864 606 531 467 449 346
335 246 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 2162 2162
Size: 5998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3946 4127 4290 3946
Size: 181 163 5654
Fragments arranged by size:
5654 181 163
BtsI GCAGTG_nn'
Cuts at: 752 1466 3239 752
Size: 714 1773 3511
Fragments arranged by size:
3511 1773 714
DraI TTT'AAA
Cuts at: 465 2406 2415 2995 465
Size: 1941 9 580 3468
Fragments arranged by size:
3468 1941 580 9
DrdI GACnn_nn'nnGTC
Cuts at: 3702 4562 3702
Size: 860 5138
EaeI y'GGCC_r
Cuts at: 1416 1496 2789 3581 3755 4146 4173 5520 1416
Size: 80 1293 792 174 391 27 1347 1894
Fragments arranged by size:
1894 1347 1293 792 391 174 80 27
EagI C'GGCC_G
Cuts at: 3581 3581
Size: 5998
EarI CTCTTCn'nnn_
Cuts at: 170 1451 1720 2596 2779 4019 4229 170
Size: 1281 269 876 183 1240 210 1939
Fragments arranged by size:
1939 1281 1240 876 269 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4204 4516 4662 5808 4204
Size: 312 146 1146 4394
Fragments arranged by size:
4394 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3008 3008
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 575 810 1306 1565 1653 1720 1744 2168
Size: 23 235 496 259 88 67 24 424
Cuts at: 2168 2682 2883 3821 4253 5002 5270 552
Size: 514 201 938 432 749 268 1280
Fragments arranged by size:
1280 938 749 514 496 432 424 268
259 235 201 88 67 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1702 1702
Size: 5998
EcoO109I rG'GnC_Cy
Cuts at: 574 778 3135 574
Size: 204 2357 3437
Fragments arranged by size:
3437 2357 204
EcoRI G'AATT_C
Cuts at: 2167 3000 2167
Size: 833 5165
EcoRV GAT'ATC
Cuts at: 1029 1289 1029
Size: 260 5738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 323 2608 4015 323
Size: 2285 1407 2306
Fragments arranged by size:
2306 2285 1407
FspI TGC'GCA
Cuts at: 3777 3777
Size: 5998
HaeII r_GCGC'y
Cuts at: 1711 1788 3430 3678 4702 5681 1711
Size: 77 1642 248 1024 979 2028
Fragments arranged by size:
2028 1642 1024 979 248 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2116 2148 2347 2379 2595 2627 3838 3868 3870
Size: 32 199 32 216 32 1211 30 2
Cuts at: 3870 3900 2116
Size: 30 4214
Fragments arranged by size:
4214 1211 216 199 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1206 3035 1206
Size: 1829 4169
HindIII A'AGCT_T
Cuts at: 903 903
Size: 5998
KasI G'GCGC_C
Cuts at: 3426 3674 3426
Size: 248 5750
KpnI G_GTAC'C
Cuts at: 3016 3016
Size: 5998
MfeI C'AATT_G
Cuts at: 438 438
Size: 5998
MluI A'CGCG_T
Cuts at: 5431 5431
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 825 1564 2989 4669 4853 825
Size: 739 1425 1680 184 1970
Fragments arranged by size:
1970 1680 1425 739 184
MscI TGG'CCA
Cuts at: 1418 1498 3757 1418
Size: 80 2259 3659
Fragments arranged by size:
3659 2259 80
MslI CAynn'nnrTG
Cuts at: 1022 1390 2364 2946 3985 4112 1022
Size: 368 974 582 1039 127 2908
Fragments arranged by size:
2908 1039 974 582 368 127
MspA1I CmG'CkG
Cuts at: 528 2545 3107 3421 3781 4796 5041 528
Size: 2017 562 314 360 1015 245 1485
Fragments arranged by size:
2017 1485 1015 562 360 314 245
NaeI GCC'GGC
Cuts at: 4177 4177
Size: 5998
NarI GG'CG_CC
Cuts at: 3427 3675 3427
Size: 248 5750
NgoMIV G'CCGG_C
Cuts at: 4175 4175
Size: 5998
NruI TCG'CGA
Cuts at: 4359 4359
Size: 5998
NsiI A_TGCA'T
Cuts at: 2943 2943
Size: 5998
NspI r_CATG'y
Cuts at: 2955 3049 3549 4458 2955
Size: 94 500 909 4495
Fragments arranged by size:
4495 909 500 94
PciI A'CATG_T
Cuts at: 4454 4454
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 2691 2691
Size: 5998
PfoI T'CCnGG_A
Cuts at: 552 2899 552
Size: 2347 3651
PmeI GTTT'AAAC
Cuts at: 2995 2995
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 436 468 5137 5169 436
Size: 32 4669 32 1265
Fragments arranged by size:
4669 1265 32 32
PpuMI rG'GwC_Cy
Cuts at: 574 778 574
Size: 204 5794
PstI C_TGCA'G
Cuts at: 1735 3043 3728 1735
Size: 1308 685 4005
Fragments arranged by size:
4005 1308 685
PvuI CG_AT'CG
Cuts at: 104 2796 104
Size: 2692 3306
PvuII CAG'CTG
Cuts at: 528 2545 3421 3781 528
Size: 2017 876 360 2745
Fragments arranged by size:
2745 2017 876 360
RsrII CG'GwC_CG
Cuts at: 4191 4191
Size: 5998
SacI G_AGCT'C
Cuts at: 3010 3010
Size: 5998
SalI G'TCGA_C
Cuts at: 3033 3033
Size: 5998
SapI GCTCTTCn'nnn_
Cuts at: 170 1451 1720 2779 4019 4229 170
Size: 1281 269 1059 1240 210 1939
Fragments arranged by size:
1939 1281 1240 1059 269 210
SbfI CC_TGCA'GG
Cuts at: 3043 3043
Size: 5998
ScaI AGT'ACT
Cuts at: 2860 5515 2860
Size: 2655 3343
SfcI C'TryA_G
Cuts at: 33 1133 1292 1731 3039 3724 4719 4910 33
Size: 1100 159 439 1308 685 995 191 1121
Fragments arranged by size:
1308 1121 1100 995 685 439 191 159
SfoI GGC'GCC
Cuts at: 3428 3676 3428
Size: 248 5750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5998
SmaI CCC'GGG
Cuts at: 3018 3018
Size: 5998
SmlI C'TyrA_G
Cuts at: 1815 2582 2828 3151 4066 4560 4822 1815
Size: 767 246 323 915 494 262 2991
Fragments arranged by size:
2991 915 767 494 323 262 246
SnaBI TAC'GTA
Cuts at: 2988 2988
Size: 5998
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5998
SphI G_CATG'C
Cuts at: 3049 3549 3049
Size: 500 5498
SspI AAT'ATT
Cuts at: 608 2011 2178 608
Size: 1403 167 4428
Fragments arranged by size:
4428 1403 167
StuI AGG'CCT
Cuts at: 491 1062 1737 491
Size: 571 675 4752
Fragments arranged by size:
4752 675 571
StyI C'CwwG_G
Cuts at: 669 1493 2891 2910 3130 3484 669
Size: 824 1398 19 220 354 3183
Fragments arranged by size:
3183 1398 824 354 220 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1579 3579 3712 4171 4382 1579
Size: 2000 133 459 211 3195
Fragments arranged by size:
3195 2000 459 211 133
TatI w'GTAC_w
Cuts at: 522 1003 2858 2978 5513 522
Size: 481 1855 120 2535 1007
Fragments arranged by size:
2535 1855 1007 481 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 221 253 1304 1336 1627 1659 5515 5547 221
Size: 32 1051 32 291 32 3856 32 672
Fragments arranged by size:
3856 1051 672 291 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3793 3793
Size: 5998
XbaI T'CTAG_A
Cuts at: 348 1524 3027 348
Size: 1176 1503 3319
Fragments arranged by size:
3319 1503 1176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3491 3491
Size: 5998
XmaI C'CCGG_G
Cuts at: 3016 3016
Size: 5998
XmnI GAAnn'nnTTC
Cuts at: 548 1360 2815 548
Size: 812 1455 3731
Fragments arranged by size:
3731 1455 812
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI BsiWI BsmBI BssHII
BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII FseI
FspAI HpaI NcoI NdeI NheI NotI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI