Restriction map for
pF1KSDA0851
(Circular) (Six-base) MAPSORT of: pF1KSDA0851.seq Check: 7984 from: 1 to: 4900
pF1KSDA0851 4900 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 303 1936 303
Size: 1633 3267
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4900
AclI AA'CG_TT
Cuts at: 4740 4740
Size: 4900
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 260 1333 1345 2723 3155 3904 4172 172
Size: 88 1073 12 1378 432 749 268 900
Fragments arranged by size:
1378 1073 900 749 432 268 88 12
AflIII A'CryG_T
Cuts at: 2250 3356 4333 2250
Size: 1106 977 2817
Fragments arranged by size:
2817 1106 977
AgeI A'CCGG_T
Cuts at: 3288 3288
Size: 4900
AhdI GACnn_n'nnGTC
Cuts at: 231 231
Size: 4900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1103 1103
Size: 4900
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1261 1293 1567 1599 1261
Size: 32 274 32 4562
Fragments arranged by size:
4562 274 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2203 3772 2203
Size: 1569 3331
ApaLI G'TGCA_C
Cuts at: 856 3670 856
Size: 2814 2086
ApoI r'AATT_y
Cuts at: 175 373 580 1411 1684 1902 4253 4509 175
Size: 198 207 831 273 218 2351 256 566
Fragments arranged by size:
2351 831 566 273 256 218 207 198
AseI AT'TA_AT
Cuts at: 20 728 20
Size: 708 4192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4900
AvaI C'yCGr_G
Cuts at: 759 893 1918 759
Size: 134 1025 3741
Fragments arranged by size:
3741 1025 134
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4900
BanI G'GyrC_C
Cuts at: 1483 1914 2328 2576 2611 1483
Size: 431 414 248 35 3772
Fragments arranged by size:
3772 431 414 248 35
BanII G_rGCy'C
Cuts at: 1063 1912 2942 1063
Size: 849 1030 3021
Fragments arranged by size:
3021 1030 849
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4652
BbsI GAAGACnn'nnnn_
Cuts at: 436 1094 1498 436
Size: 658 404 3838
Fragments arranged by size:
3838 658 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1271 1305 1271
Size: 34 4866
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3559 4900 2796
Size: 763 1341 2796
Fragments arranged by size:
2796 1341 763
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4900
BfrBI ATG'CAT
Cuts at: 714 714
Size: 4900
BglII A'GATC_T
Cuts at: 2413 4452 2413
Size: 2039 2861
BlpI GC'TnA_GC
Cuts at: 2010 2010
Size: 4900
Bme1580I G_kGCm'C
Cuts at: 313 860 2523 2616 3674 313
Size: 547 1663 93 1058 1539
Fragments arranged by size:
1663 1539 1058 547 93
BmrI ACTGGGnnnn_n'
Cuts at: 316 2110 2191 2526 4672 316
Size: 1794 81 335 2146 544
Fragments arranged by size:
2146 1794 544 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1096 2074 2953 3447 3745 919
Size: 177 978 879 494 298 2074
Fragments arranged by size:
2074 978 879 494 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 4604 4604
Size: 4900
BsaAI yAC'GTr
Cuts at: 1890 2251 1890
Size: 361 4539
BsaBI GATnn'nnATC
Cuts at: 6 754 2412 4451 6
Size: 748 1658 2039 455
Fragments arranged by size:
2039 1658 748 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3281 4777 2329
Size: 248 704 1496 2452
Fragments arranged by size:
2452 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2087 2608 3288 3562 3709 4120 2087
Size: 521 680 274 147 411 2867
Fragments arranged by size:
2867 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 756 786 756
Size: 30 4870
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 777 780 777
Size: 3 4897
BseYI C'CCAG_C
Cuts at: 2323 3081 3660 4156 2323
Size: 758 579 496 3067
Fragments arranged by size:
3067 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 641
Size: 4900
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3696 104
Size: 2336 46 1210 1308
Fragments arranged by size:
2336 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 860 1787 1912 2690 2880 3674 860
Size: 927 125 778 190 794 2086
Fragments arranged by size:
2086 927 794 778 190 125
BsmI GAATG_Cn'
Cuts at: 714 714
Size: 4900
Bsp1286I G_dGCh'C
Cuts at: 313 860 1063 1787 1912 2523 2616 2690 2880
Size: 547 203 724 125 611 93 74 190
Cuts at: 2880 2942 3674 313
Size: 62 732 1539
Fragments arranged by size:
1539 732 724 611 547 203 190 125
93 74 62
BspEI T'CCGG_A
Cuts at: 4120 4120
Size: 4900
BspHI T'CATG_A
Cuts at: 4076 4076
Size: 4900
BspMI ACCTGCnnnn'nnnn_
Cuts at: 319 1948 2162 2464 2845 319
Size: 1629 214 302 381 2374
Fragments arranged by size:
2374 1629 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4730 4895 50
Size: 3140 54 1486 165 55
Fragments arranged by size:
3140 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1130 2810 1130
Size: 1680 3220
BsrFI r'CCGG_y
Cuts at: 2896 3077 3288 2896
Size: 181 211 4508
Fragments arranged by size:
4508 211 181
BsrGI T'GTAC_A
Cuts at: 885 885
Size: 4900
BssSI C'ACGA_G
Cuts at: 3169 3529 3169
Size: 360 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 2275 2275
Size: 4900
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4900
BstEII G'GTnAC_C
Cuts at: 243 243
Size: 4900
BstXI CCAn_nnnn'nTGG
Cuts at: 1679 1679
Size: 4900
BstYI r'GATC_y
Cuts at: 1 1923 2390 2413 2748 2994 4008 4094 4106
Size: 1922 467 23 335 246 1014 86 12
Cuts at: 4106 4452 1
Size: 346 449
Fragments arranged by size:
1922 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 304 304
Size: 4900
BtgI C'CryG_G
Cuts at: 125 125
Size: 4900
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 499 2848 3029 3192 499
Size: 2349 181 163 2207
Fragments arranged by size:
2349 2207 181 163
BtsI GCAGTG_nn'
Cuts at: 946 2141 946
Size: 1195 3705
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4900
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3464 2604
Size: 860 4040
EaeI y'GGCC_r
Cuts at: 1248 2483 2657 3048 3075 4422 1248
Size: 1235 174 391 27 1347 1726
Fragments arranged by size:
1726 1347 1235 391 174 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4900
EarI CTCTTCn'nnn_
Cuts at: 1792 2921 3131 1792
Size: 1129 210 3561
Fragments arranged by size:
3561 1129 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3418 3564 4710 3106
Size: 312 146 1146 3296
Fragments arranged by size:
3296 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 260 1333 1345 2723 3155 3904 4172 172
Size: 88 1073 12 1378 432 749 268 900
Fragments arranged by size:
1378 1073 900 749 432 268 88 12
EcoO109I rG'GnC_Cy
Cuts at: 2037 2037
Size: 4900
EcoRI G'AATT_C
Cuts at: 580 1902 580
Size: 1322 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1515 1596 2917 1515
Size: 81 1321 3498
Fragments arranged by size:
3498 1321 81
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4900
HaeII r_GCGC'y
Cuts at: 2332 2580 3604 4583 2332
Size: 248 1024 979 2649
Fragments arranged by size:
2649 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 917 949 1158 1190 2740 2770 2772
Size: 32 668 32 209 32 1550 30 2
Cuts at: 2772 2802 217
Size: 30 2315
Fragments arranged by size:
2315 1550 668 209 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1937 1937
Size: 4900
HindIII A'AGCT_T
Cuts at: 188 875 1393 188
Size: 687 518 3695
Fragments arranged by size:
3695 687 518
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4652
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4900
MfeI C'AATT_G
Cuts at: 1287 1287
Size: 4900
MluI A'CGCG_T
Cuts at: 4333 4333
Size: 4900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 3571 3755 590
Size: 2981 184 1735
Fragments arranged by size:
2981 1735 184
MscI TGG'CCA
Cuts at: 1250 2659 1250
Size: 1409 3491
MslI CAynn'nnrTG
Cuts at: 863 2887 3014 863
Size: 2024 127 2749
Fragments arranged by size:
2749 2024 127
MspA1I CmG'CkG
Cuts at: 150 280 558 618 2009 2323 2683 3698 3943
Size: 130 278 60 1391 314 360 1015 245
Cuts at: 3943 150
Size: 1107
Fragments arranged by size:
1391 1107 1015 360 314 278 245 130
60
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4900
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4652
NcoI C'CATG_G
Cuts at: 125 125
Size: 4900
NdeI CA'TA_TG
Cuts at: 534 534
Size: 4900
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4900
NruI TCG'CGA
Cuts at: 3261 3261
Size: 4900
NsiI A_TGCA'T
Cuts at: 716 716
Size: 4900
NspI r_CATG'y
Cuts at: 1384 1951 2451 3360 1384
Size: 567 500 909 2924
Fragments arranged by size:
2924 909 567 500
PciI A'CATG_T
Cuts at: 3356 3356
Size: 4900
PflMI CCAn_nnn'nTGG
Cuts at: 298 324 1257 298
Size: 26 933 3941
Fragments arranged by size:
3941 933 26
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4039 4071 4039
Size: 32 4868
PsiI TTA'TAA
Cuts at: 425 1036 1537 1571 425
Size: 611 501 34 3754
Fragments arranged by size:
3754 611 501 34
PspXI vC'TCGA_Gb
Cuts at: 893 893
Size: 4900
PstI C_TGCA'G
Cuts at: 1945 2630 1945
Size: 685 4215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4900
PvuII CAG'CTG
Cuts at: 150 280 2323 2683 150
Size: 130 2043 360 2367
Fragments arranged by size:
2367 2043 360 130
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4900
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4900
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4900
SapI GCTCTTCn'nnn_
Cuts at: 1792 2921 3131 1792
Size: 1129 210 3561
Fragments arranged by size:
3561 1129 210
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4900
ScaI AGT'ACT
Cuts at: 4417 4417
Size: 4900
SfcI C'TryA_G
Cuts at: 33 1941 2626 3621 3812 33
Size: 1908 685 995 191 1121
Fragments arranged by size:
1908 1121 995 685 191
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4652
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4900
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4900
SmlI C'TyrA_G
Cuts at: 759 893 934 1075 2053 2968 3462 3724 759
Size: 134 41 141 978 915 494 262 1935
Fragments arranged by size:
1935 978 915 494 262 141 134 41
SnaBI TAC'GTA
Cuts at: 1890 1890
Size: 4900
SpeI A'CTAG_T
Cuts at: 67 571 67
Size: 504 4396
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4400
SspI AAT'ATT
Cuts at: 296 296
Size: 4900
StyI C'CwwG_G
Cuts at: 125 1106 1672 2032 2386 125
Size: 981 566 360 354 2639
Fragments arranged by size:
2639 981 566 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2481 2614 3073 3284 2481
Size: 133 459 211 4097
Fragments arranged by size:
4097 459 211 133
TatI w'GTAC_w
Cuts at: 885 938 4415 885
Size: 53 3477 1370
Fragments arranged by size:
3477 1370 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4417 4449 4417
Size: 32 4868
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4900
XbaI T'CTAG_A
Cuts at: 1302 1929 1302
Size: 627 4273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 2393
Size: 4900
XhoI C'TCGA_G
Cuts at: 759 893 759
Size: 134 4766
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4900
XmnI GAAnn'nnTTC
Cuts at: 810 1629 810
Size: 819 4081
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PciI PflMI PmeI PpiI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BpmI Bpu10I BsiWI
BsmBI BssHII Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI ZraI