Restriction map for
pF1KSDA0854
(Circular) (Six-base) MAPSORT of: pF1KSDA0854.seq Check: 9928 from: 1 to: 5648
pF1KSDA0854 5648 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 206 416 2686 206
Size: 210 2270 3168
Fragments arranged by size:
3168 2270 210
Acc65I G'GTAC_C
Cuts at: 2664 2664
Size: 5648
AclI AA'CG_TT
Cuts at: 5488 5488
Size: 5648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 3473 3905 4652 4920 568
Size: 2905 432 747 268 1296
Fragments arranged by size:
2905 1296 747 432 268
AfeI AGC'GCT
Cuts at: 1066 1066
Size: 5648
AflIII A'CryG_T
Cuts at: 455 846 3000 4104 5081 455
Size: 391 2154 1104 977 1022
Fragments arranged by size:
2154 1104 1022 977 391
AgeI A'CCGG_T
Cuts at: 2435 4036 2435
Size: 1601 4047
AleI CACnn'nnGTG
Cuts at: 1192 1192
Size: 5648
AlwNI CAG_nnn'CTG
Cuts at: 1049 2626 2953 4520 1049
Size: 1577 327 1567 2177
Fragments arranged by size:
2177 1577 1567 327
ApaLI G'TGCA_C
Cuts at: 4418 4418
Size: 5648
ApoI r'AATT_y
Cuts at: 2652 5001 5257 2652
Size: 2349 256 3043
Fragments arranged by size:
3043 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5648
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5648
AvaI C'yCGr_G
Cuts at: 196 526 733 748 2668 196
Size: 330 207 15 1920 3176
Fragments arranged by size:
3176 1920 330 207 15
BamHI G'GATC_C
Cuts at: 2673 2673
Size: 5648
BanI G'GyrC_C
Cuts at: 720 1132 1401 1981 2664 3078 3326 3361 720
Size: 412 269 580 683 414 248 35 3007
Fragments arranged by size:
3007 683 580 414 412 269 248 35
BanII G_rGCy'C
Cuts at: 827 1412 1944 2284 2662 3692 827
Size: 585 532 340 378 1030 2783
Fragments arranged by size:
2783 1030 585 532 378 340
BbeI G_GCGC'C
Cuts at: 3082 3330 3082
Size: 248 5400
BbsI GAAGACnn'nnnn_
Cuts at: 1468 1766 1468
Size: 298 5350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1624 1658 1624
Size: 34 5614
BciVI GTATCCnnnnn_n'
Cuts at: 933 3546 4307 5648 933
Size: 2613 761 1341 933
Fragments arranged by size:
2613 1341 933 761
BclI T'GATC_A
Cuts at: 956 2229 3168 956
Size: 1273 939 3436
Fragments arranged by size:
3436 1273 939
BfrBI ATG'CAT
Cuts at: 158 158
Size: 5648
BglI GCCn_nnn'nGGC
Cuts at: 1180 1666 2459 1180
Size: 486 793 4369
Fragments arranged by size:
4369 793 486
BglII A'GATC_T
Cuts at: 3163 5200 3163
Size: 2037 3611
BlpI GC'TnA_GC
Cuts at: 1828 2760 1828
Size: 932 4716
Bme1580I G_kGCm'C
Cuts at: 197 725 893 1986 3273 3366 4422 197
Size: 528 168 1093 1287 93 1056 1423
Fragments arranged by size:
1423 1287 1093 1056 528 168 93
BmgBI CAC'GTC
Cuts at: 249 849 249
Size: 600 5048
BmrI ACTGGGnnnn_n'
Cuts at: 1170 1980 2070 2860 2941 3276 5420 1170
Size: 810 90 790 81 335 2144 1398
Fragments arranged by size:
2144 1398 810 790 335 90 81
BmtI G_CTAG'C
Cuts at: 134 134
Size: 5648
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 819 1863 2445 4015 819
Size: 1044 582 1570 2452
Fragments arranged by size:
2452 1570 1044 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1486 2577 1195
Size: 291 1091 4266
Fragments arranged by size:
4266 1091 291
Bpu10I CC'TnA_GC
Cuts at: 1397 1810 1397
Size: 413 5235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 1471 2824 3703 4195 4493 789
Size: 682 1353 879 492 298 1944
Fragments arranged by size:
1944 1353 879 682 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1338 1813 5352 1338
Size: 475 3539 1634
Fragments arranged by size:
3539 1634 475
BsaAI yAC'GTr
Cuts at: 456 759 1688 2505 2640 3001 456
Size: 303 929 817 135 361 3103
Fragments arranged by size:
3103 929 817 361 303 135
BsaBI GATnn'nnATC
Cuts at: 6 3162 5199 6
Size: 3156 2037 455
Fragments arranged by size:
3156 2037 455
BsaHI Gr'CG_yC
Cuts at: 3079 3327 4029 5525 3079
Size: 248 702 1496 3202
Fragments arranged by size:
3202 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1978 2435 2837 3358 4036 4310 4457 4868 1978
Size: 457 402 521 678 274 147 411 2758
Fragments arranged by size:
2758 678 521 457 411 402 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 1960 1990 496
Size: 30 1434 30 4154
Fragments arranged by size:
4154 1434 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 817 1048 1120 2070 2497 2539 751
Size: 66 231 72 950 427 42 3860
Fragments arranged by size:
3860 950 427 231 72 66 42
BseYI C'CCAG_C
Cuts at: 1061 2005 3073 3831 4408 4904 1061
Size: 944 1068 758 577 496 1805
Fragments arranged by size:
1805 1068 944 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 482
Size: 5648
BsiEI CG_ry'CG
Cuts at: 104 1683 2519 3190 3236 4444 104
Size: 1579 836 671 46 1208 1308
Fragments arranged by size:
1579 1308 1208 836 671 46
BsiHKAI G_wGCw'C
Cuts at: 827 1040 1956 2062 2662 3440 3630 4422 827
Size: 213 916 106 600 778 190 792 2053
Fragments arranged by size:
2053 916 792 778 600 213 190 106
BsmBI CGTCTCn'nnnn_
Cuts at: 1293 1293
Size: 5648
Bsp1286I G_dGCh'C
Cuts at: 197 725 827 893 1040 1412 1944 1956 1986
Size: 528 102 66 147 372 532 12 30
Cuts at: 1986 2062 2284 2662 3273 3366 3440 3630 3692
Size: 76 222 378 611 93 74 190 62
Cuts at: 3692 4422 197
Size: 730 1423
Fragments arranged by size:
1423 730 611 532 528 378 372 222
190 147 102 93 76 74 66 62
30 12
BspEI T'CCGG_A
Cuts at: 4868 4868
Size: 5648
BspHI T'CATG_A
Cuts at: 683 4824 683
Size: 4141 1507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2698 2912 3214 3595 2698
Size: 214 302 381 4751
Fragments arranged by size:
4751 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1437 3940 3994 5478 5643 50
Size: 1387 2503 54 1484 165 55
Fragments arranged by size:
2503 1484 1387 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3560 3560
Size: 5648
BsrFI r'CCGG_y
Cuts at: 1535 2435 3646 3827 4036 1535
Size: 900 1211 181 209 3147
Fragments arranged by size:
3147 1211 900 209 181
BsrGI T'GTAC_A
Cuts at: 861 861
Size: 5648
BssSI C'ACGA_G
Cuts at: 3919 4277 3919
Size: 358 5290
BstAPI GCAn_nnn'nTGC
Cuts at: 371 3025 371
Size: 2654 2994
BstBI TT'CG_AA
Cuts at: 110 4009 110
Size: 3899 1749
BstEII G'GTnAC_C
Cuts at: 1266 2361 1266
Size: 1095 4553
BstYI r'GATC_y
Cuts at: 1 659 797 1232 2673 3140 3163 3498 3744
Size: 658 138 435 1441 467 23 335 246
Cuts at: 3744 4756 4842 4854 5200 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1441 1012 658 467 449 435 346 335
246 138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2053 2053
Size: 5648
BtgI C'CryG_G
Cuts at: 125 1313 1332 1907 2219 2270 125
Size: 1188 19 575 312 51 3503
Fragments arranged by size:
3503 1188 575 312 51 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1377 1647 3598 3779 3942 1377
Size: 270 1951 181 163 3083
Fragments arranged by size:
3083 1951 270 181 163
BtsI GCAGTG_nn'
Cuts at: 2891 2891
Size: 5648
DraI TTT'AAA
Cuts at: 2647 2647
Size: 5648
DrdI GACnn_nn'nnGTC
Cuts at: 1275 3354 4212 1275
Size: 2079 858 2711
Fragments arranged by size:
2711 2079 858
EaeI y'GGCC_r
Cuts at: 251 1239 1335 1424 1658 2111 2273 2460 2628
Size: 988 96 89 234 453 162 187 168
Cuts at: 2628 3233 3407 3798 3825 5170 251
Size: 605 174 391 27 1345 729
Fragments arranged by size:
1345 988 729 605 453 391 234 187
174 168 162 96 89 27
EagI C'GGCC_G
Cuts at: 3233 3233
Size: 5648
EarI CTCTTCn'nnn_
Cuts at: 291 1091 1336 2336 3671 3881 291
Size: 800 245 1000 1335 210 2058
Fragments arranged by size:
2058 1335 1000 800 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1881 3856 4166 4312 5458 1881
Size: 1975 310 146 1146 2071
Fragments arranged by size:
2071 1975 1146 310 146
EcoICRI GAG'CTC
Cuts at: 825 2660 825
Size: 1835 3813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1195 1486 2577 3473 3905 4652 4920 568
Size: 627 291 1091 896 432 747 268 1296
Fragments arranged by size:
1296 1091 896 747 627 432 291 268
EcoO109I rG'GnC_Cy
Cuts at: 897 2322 2787 897
Size: 1425 465 3758
Fragments arranged by size:
3758 1425 465
EcoRI G'AATT_C
Cuts at: 2652 2652
Size: 5648
EcoRV GAT'ATC
Cuts at: 1600 1600
Size: 5648
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1365 1512 3667 1365
Size: 147 2155 3346
Fragments arranged by size:
3346 2155 147
FspI TGC'GCA
Cuts at: 3429 3429
Size: 5648
HaeII r_GCGC'y
Cuts at: 1068 3082 3330 4352 5331 1068
Size: 2014 248 1022 979 1385
Fragments arranged by size:
2014 1385 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 484 496 516 528 1467 1499 1960
Size: 32 151 12 20 12 939 32 461
Cuts at: 1960 1992 2521 2553 3490 3520 3522 3552 301
Size: 32 529 32 937 30 2 30 2397
Fragments arranged by size:
2397 939 937 529 461 151 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 417 1280 1991 2687 417
Size: 863 711 696 3378
Fragments arranged by size:
3378 863 711 696
HindIII A'AGCT_T
Cuts at: 1870 1870
Size: 5648
HpaI GTT'AAC
Cuts at: 1991 1991
Size: 5648
KasI G'GCGC_C
Cuts at: 3078 3326 3078
Size: 248 5400
KpnI G_GTAC'C
Cuts at: 2668 2668
Size: 5648
MfeI C'AATT_G
Cuts at: 2021 2021
Size: 5648
MluI A'CGCG_T
Cuts at: 5081 5081
Size: 5648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 527 2564 2630 4319 4503 527
Size: 2037 66 1689 184 1672
Fragments arranged by size:
2037 1689 1672 184 66
MscI TGG'CCA
Cuts at: 253 1337 1426 1660 2630 3409 253
Size: 1084 89 234 970 779 2492
Fragments arranged by size:
2492 1084 970 779 234 89
MslI CAynn'nnrTG
Cuts at: 1162 1192 1546 3637 3764 1162
Size: 30 354 2091 127 3046
Fragments arranged by size:
3046 2091 354 127 30
MspA1I CmG'CkG
Cuts at: 793 867 1015 1085 1274 2759 3073 3433 4446
Size: 74 148 70 189 1485 314 360 1013
Cuts at: 4446 4691 793
Size: 245 1750
Fragments arranged by size:
1750 1485 1013 360 314 245 189 148
74 70
NaeI GCC'GGC
Cuts at: 3829 3829
Size: 5648
NarI GG'CG_CC
Cuts at: 3079 3327 3079
Size: 248 5400
NcoI C'CATG_G
Cuts at: 125 1332 1907 2219 2270 125
Size: 1207 575 312 51 3503
Fragments arranged by size:
3503 1207 575 312 51
NgoMIV G'CCGG_C
Cuts at: 3827 3827
Size: 5648
NheI G'CTAG_C
Cuts at: 130 130
Size: 5648
NsiI A_TGCA'T
Cuts at: 160 160
Size: 5648
NspI r_CATG'y
Cuts at: 415 423 2701 3201 4108 415
Size: 8 2278 500 907 1955
Fragments arranged by size:
2278 1955 907 500 8
PacI TTA_AT'TAA
Cuts at: 557 557
Size: 5648
PciI A'CATG_T
Cuts at: 4104 4104
Size: 5648
PflMI CCAn_nnn'nTGG
Cuts at: 1935 2220 1935
Size: 285 5363
PmeI GTTT'AAAC
Cuts at: 2647 2647
Size: 5648
PmlI CAC'GTG
Cuts at: 759 759
Size: 5648
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 4787
Size: 32 5616
PpuMI rG'GwC_Cy
Cuts at: 897 2322 897
Size: 1425 4223
PstI C_TGCA'G
Cuts at: 2622 2695 3380 2622
Size: 73 685 4890
Fragments arranged by size:
4890 685 73
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5648
PvuII CAG'CTG
Cuts at: 793 867 1015 1085 3073 3433 793
Size: 74 148 70 1988 360 3008
Fragments arranged by size:
3008 1988 360 148 74 70
RsrII CG'GwC_CG
Cuts at: 3843 3843
Size: 5648
SacI G_AGCT'C
Cuts at: 827 2662 827
Size: 1835 3813
SalI G'TCGA_C
Cuts at: 415 2685 415
Size: 2270 3378
SapI GCTCTTCn'nnn_
Cuts at: 3671 3881 3671
Size: 210 5438
SbfI CC_TGCA'GG
Cuts at: 2695 2695
Size: 5648
ScaI AGT'ACT
Cuts at: 5165 5165
Size: 5648
SfcI C'TryA_G
Cuts at: 33 2618 2691 3376 4369 4560 33
Size: 2585 73 685 993 191 1121
Fragments arranged by size:
2585 1121 993 685 191 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 1666 1666
Size: 5648
SfoI GGC'GCC
Cuts at: 3080 3328 3080
Size: 248 5400
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5648
SmaI CCC'GGG
Cuts at: 528 2670 528
Size: 2142 3506
SmlI C'TyrA_G
Cuts at: 804 1486 2803 3718 4210 4472 804
Size: 682 1317 915 492 262 1980
Fragments arranged by size:
1980 1317 915 682 492 262
SnaBI TAC'GTA
Cuts at: 2640 2640
Size: 5648
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5648
SphI G_CATG'C
Cuts at: 2701 3201 2701
Size: 500 5148
SspI AAT'ATT
Cuts at: 2314 2314
Size: 5648
StuI AGG'CCT
Cuts at: 2635 2635
Size: 5648
StyI C'CwwG_G
Cuts at: 125 254 348 893 1332 1349 1418 1907 2219
Size: 129 94 545 439 17 69 489 312
Cuts at: 2219 2270 2782 3136 125
Size: 51 512 354 2637
Fragments arranged by size:
2637 545 512 489 439 354 312 129
94 69 51 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1043 1075 1431 3231 3364 3823 4032 1043
Size: 32 356 1800 133 459 209 2659
Fragments arranged by size:
2659 1800 459 356 209 133 32
TatI w'GTAC_w
Cuts at: 861 5163 861
Size: 4302 1346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5165 5197 5165
Size: 32 5616
Tth111I GACn'n_nGTC
Cuts at: 1679 2611 3445 1679
Size: 932 834 3882
Fragments arranged by size:
3882 932 834
XbaI T'CTAG_A
Cuts at: 2679 2679
Size: 5648
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1653 2121 3143 1653
Size: 468 1022 4158
Fragments arranged by size:
4158 1022 468
XmaI C'CCGG_G
Cuts at: 526 2668 526
Size: 2142 3506
XmnI GAAnn'nnTTC
Cuts at: 400 1123 1582 2042 400
Size: 723 459 460 4006
Fragments arranged by size:
4006 723 460 459
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BsiWI BsmI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI NdeI NotI
NruI PasI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SrfI SwaI XhoI ZraI