Restriction map for
pF1KSDA0863
(Circular) (Six-base) MAPSORT of: pF1KSDA0863.seq Check: 4247 from: 1 to: 6530
pF1KSDA0863 6530 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2761 2761
Size: 6530
AccI GT'mk_AC
Cuts at: 2246 2642 2888 3568 2246
Size: 396 246 680 5208
Fragments arranged by size:
5208 680 396 246
Acc65I G'GTAC_C
Cuts at: 3546 3546
Size: 6530
AclI AA'CG_TT
Cuts at: 6370 6370
Size: 6530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 480 2869 3217 4355 4787 5534 5802 250
Size: 230 2389 348 1138 432 747 268 978
Fragments arranged by size:
2389 1138 978 747 432 348 268 230
AfeI AGC'GCT
Cuts at: 978 978
Size: 6530
AflIII A'CryG_T
Cuts at: 592 3882 4986 5963 592
Size: 3290 1104 977 1159
Fragments arranged by size:
3290 1159 1104 977
AgeI A'CCGG_T
Cuts at: 4918 4918
Size: 6530
AhdI GACnn_n'nnGTC
Cuts at: 3214 3214
Size: 6530
AleI CACnn'nnGTG
Cuts at: 2879 2999 2879
Size: 120 6410
AlwNI CAG_nnn'CTG
Cuts at: 1054 2055 3835 5402 1054
Size: 1001 1780 1567 2182
Fragments arranged by size:
2182 1780 1567 1001
ApaLI G'TGCA_C
Cuts at: 2330 5300 2330
Size: 2970 3560
ApoI r'AATT_y
Cuts at: 134 1740 3534 5883 6139 134
Size: 1606 1794 2349 256 525
Fragments arranged by size:
2349 1794 1606 525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6530
AvaI C'yCGr_G
Cuts at: 2725 3141 3550 2725
Size: 416 409 5705
Fragments arranged by size:
5705 416 409
BamHI G'GATC_C
Cuts at: 3555 3555
Size: 6530
BanI G'GyrC_C
Cuts at: 1066 1840 2501 2682 2719 2816 3546 3960 4208
Size: 774 661 181 37 97 730 414 248
Cuts at: 4208 4243 1066
Size: 35 3353
Fragments arranged by size:
3353 774 730 661 414 248 181 97
37 35
BanII G_rGCy'C
Cuts at: 1393 2231 3544 4574 1393
Size: 838 1313 1030 3349
Fragments arranged by size:
3349 1313 1030 838
BbeI G_GCGC'C
Cuts at: 3964 4212 3964
Size: 248 6282
BbsI GAAGACnn'nnnn_
Cuts at: 2212 3095 2212
Size: 883 5647
BbvCI CC'TCA_GC
Cuts at: 1181 1710 2714 1181
Size: 529 1004 4997
Fragments arranged by size:
4997 1004 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3150 3184 3150
Size: 34 6496
BciVI GTATCCnnnnn_n'
Cuts at: 2657 4428 5189 6530 2657
Size: 1771 761 1341 2657
Fragments arranged by size:
2657 1771 1341 761
BclI T'GATC_A
Cuts at: 4050 4050
Size: 6530
BfrBI ATG'CAT
Cuts at: 2427 3033 2427
Size: 606 5924
BglI GCCn_nnn'nGGC
Cuts at: 1806 1806
Size: 6530
BglII A'GATC_T
Cuts at: 868 4045 6082 868
Size: 3177 2037 1316
Fragments arranged by size:
3177 2037 1316
BlpI GC'TnA_GC
Cuts at: 3642 3642
Size: 6530
Bme1580I G_kGCm'C
Cuts at: 1071 1843 2334 2722 4155 4248 5304 1071
Size: 772 491 388 1433 93 1056 2297
Fragments arranged by size:
2297 1433 1056 772 491 388 93
BmrI ACTGGGnnnn_n'
Cuts at: 3742 3823 4158 6302 3742
Size: 81 335 2144 3970
Fragments arranged by size:
3970 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4897 4897
Size: 6530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 1113 1484 2030 2096 965
Size: 148 371 546 66 5399
Fragments arranged by size:
5399 546 371 148 66
Bpu10I CC'TnA_GC
Cuts at: 1181 1710 2714 1181
Size: 529 1004 4997
Fragments arranged by size:
4997 1004 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1266 2326 3706 4585 5077 5375 1266
Size: 1060 1380 879 492 298 2421
Fragments arranged by size:
2421 1380 1060 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1131 1866 2397 6234 1131
Size: 735 531 3837 1427
Fragments arranged by size:
3837 1427 735 531
BsaAI yAC'GTr
Cuts at: 3522 3883 3522
Size: 361 6169
BsaBI GATnn'nnATC
Cuts at: 6 2044 4044 6081 6
Size: 2038 2000 2037 455
Fragments arranged by size:
2038 2037 2000 455
BsaHI Gr'CG_yC
Cuts at: 3961 4209 4911 6407 3961
Size: 248 702 1496 4084
Fragments arranged by size:
4084 1496 702 248
BsaWI w'CCGG_w
Cuts at: 518 3719 4240 4918 5192 5339 5750 518
Size: 3201 521 678 274 147 411 1298
Fragments arranged by size:
3201 1298 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2013 2043 2013
Size: 30 6500
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1695 1698 2099 2145 2148 3121 1695
Size: 3 401 46 3 973 5104
Fragments arranged by size:
5104 973 401 46 3 3
BseYI C'CCAG_C
Cuts at: 1032 1173 1337 2659 2978 3955 4713 5290 5786
Size: 141 164 1322 319 977 758 577 496
Cuts at: 5786 1032
Size: 1776
Fragments arranged by size:
1776 1322 977 758 577 496 319 164
141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 2057 2087 935
Size: 1122 30 5378
Fragments arranged by size:
5378 1122 30
BsiEI CG_ry'CG
Cuts at: 104 4072 4118 5326 104
Size: 3968 46 1208 1308
Fragments arranged by size:
3968 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 972 2186 2231 2334 2489 2734 2949 3544 4322
Size: 1214 45 103 155 245 215 595 778
Cuts at: 4322 4512 5304 972
Size: 190 792 2198
Fragments arranged by size:
2198 1214 792 778 595 245 215 190
155 103 45
BsmI GAATG_Cn'
Cuts at: 2130 2130
Size: 6530
Bsp1286I G_dGCh'C
Cuts at: 972 1071 1393 1843 2186 2231 2334 2489 2722
Size: 99 322 450 343 45 103 155 233
Cuts at: 2722 2734 2949 3544 4155 4248 4322 4512 4574
Size: 12 215 595 611 93 74 190 62
Cuts at: 4574 5304 972
Size: 730 2198
Fragments arranged by size:
2198 730 611 595 450 343 322 233
215 190 155 103 99 93 74 62
45 12
BspEI T'CCGG_A
Cuts at: 518 5750 518
Size: 5232 1298
BspHI T'CATG_A
Cuts at: 5706 5706
Size: 6530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2218 2585 2761 2978 3580 3794 4096 4477 2218
Size: 367 176 217 602 214 302 381 4271
Fragments arranged by size:
4271 602 381 367 302 217 214 176
BsrBI CCG'CTC
Cuts at: 50 2634 4822 4876 6360 6525 50
Size: 2584 2188 54 1484 165 55
Fragments arranged by size:
2584 2188 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 920 4442 920
Size: 3522 3008
BsrFI r'CCGG_y
Cuts at: 1998 2083 2734 4528 4709 4918 1998
Size: 85 651 1794 181 209 3610
Fragments arranged by size:
3610 1794 651 209 181 85
BssSI C'ACGA_G
Cuts at: 4801 5159 4801
Size: 358 6172
BstAPI GCAn_nnn'nTGC
Cuts at: 2329 2876 3907 2329
Size: 547 1031 4952
Fragments arranged by size:
4952 1031 547
BstBI TT'CG_AA
Cuts at: 110 4891 110
Size: 4781 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 483 2679 2881 3100 483
Size: 2196 202 219 3913
Fragments arranged by size:
3913 2196 219 202
BstYI r'GATC_y
Cuts at: 1 749 868 3249 3555 4022 4045 4380 4626
Size: 748 119 2381 306 467 23 335 246
Cuts at: 4626 5638 5724 5736 6082 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2381 1012 748 467 449 346 335 306
246 119 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 590 2382 2901 4480 4661 4824 590
Size: 1792 519 1579 181 163 2296
Fragments arranged by size:
2296 1792 1579 519 181 163
BtsI GCAGTG_nn'
Cuts at: 972 2206 2874 3773 972
Size: 1234 668 899 3729
Fragments arranged by size:
3729 1234 899 668
DraI TTT'AAA
Cuts at: 3177 3529 3177
Size: 352 6178
DrdI GACnn_nn'nnGTC
Cuts at: 1605 4236 5094 1605
Size: 2631 858 3041
Fragments arranged by size:
3041 2631 858
EaeI y'GGCC_r
Cuts at: 1104 1560 2081 3072 4115 4289 4680 4707 6052
Size: 456 521 991 1043 174 391 27 1345
Cuts at: 6052 1104
Size: 1582
Fragments arranged by size:
1582 1345 1043 991 521 456 391 174
27
EagI C'GGCC_G
Cuts at: 4115 4115
Size: 6530
EarI CTCTTCn'nnn_
Cuts at: 2396 3236 4553 4763 2396
Size: 840 1317 210 4163
Fragments arranged by size:
4163 1317 840 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2021 4738 5048 5194 6340 2021
Size: 2717 310 146 1146 2211
Fragments arranged by size:
2717 2211 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2229 3542 2229
Size: 1313 5217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 480 965 1113 1484 2030 2096 2869 3217
Size: 230 485 148 371 546 66 773 348
Cuts at: 3217 4355 4787 5534 5802 250
Size: 1138 432 747 268 978
Fragments arranged by size:
1138 978 773 747 546 485 432 371
348 268 230 148 66
EcoO109I rG'GnC_Cy
Cuts at: 234 1348 1410 3208 3669 234
Size: 1114 62 1798 461 3095
Fragments arranged by size:
3095 1798 1114 461 62
EcoRI G'AATT_C
Cuts at: 1740 3534 1740
Size: 1794 4736
EcoRV GAT'ATC
Cuts at: 328 2469 328
Size: 2141 4389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 208 4549 208
Size: 4341 2189
FspI TGC'GCA
Cuts at: 4311 4311
Size: 6530
HaeII r_GCGC'y
Cuts at: 980 3964 4212 5234 6213 980
Size: 2984 248 1022 979 1297
Fragments arranged by size:
2984 1297 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1916 1948 2013 2045 2275 2307 2947 2979 3200
Size: 32 65 32 230 32 640 32 221
Cuts at: 3200 3232 4372 4402 4404 4434 1916
Size: 32 1140 30 2 30 4012
Fragments arranged by size:
4012 1140 640 230 221 65 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3569 3569
Size: 6530
HindIII A'AGCT_T
Cuts at: 862 2623 862
Size: 1761 4769
KasI G'GCGC_C
Cuts at: 3960 4208 3960
Size: 248 6282
KpnI G_GTAC'C
Cuts at: 3550 3550
Size: 6530
MluI A'CGCG_T
Cuts at: 5963 5963
Size: 6530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1260 1987 2264 5201 5385 1239
Size: 21 727 277 2937 184 2384
Fragments arranged by size:
2937 2384 727 277 184 21
MscI TGG'CCA
Cuts at: 1106 1562 4291 1106
Size: 456 2729 3345
Fragments arranged by size:
3345 2729 456
MslI CAynn'nnrTG
Cuts at: 416 2879 2999 4519 4646 416
Size: 2463 120 1520 127 2300
Fragments arranged by size:
2463 2300 1520 127 120
MspA1I CmG'CkG
Cuts at: 215 1714 2071 3096 3641 3955 4315 5328 5573
Size: 1499 357 1025 545 314 360 1013 245
Cuts at: 5573 215
Size: 1172
Fragments arranged by size:
1499 1172 1025 1013 545 360 357 314
245
NaeI GCC'GGC
Cuts at: 4711 4711
Size: 6530
NarI GG'CG_CC
Cuts at: 3961 4209 3961
Size: 248 6282
NgoMIV G'CCGG_C
Cuts at: 4709 4709
Size: 6530
NsiI A_TGCA'T
Cuts at: 2429 3035 2429
Size: 606 5924
NspI r_CATG'y
Cuts at: 596 646 2103 3583 4083 4990 596
Size: 50 1457 1480 500 907 2136
Fragments arranged by size:
2136 1480 1457 907 500 50
PacI TTA_AT'TAA
Cuts at: 674 674
Size: 6530
PasI CC'CwG_GG
Cuts at: 1062 1318 1405 1525 1062
Size: 256 87 120 6067
Fragments arranged by size:
6067 256 120 87
PciI A'CATG_T
Cuts at: 592 4986 592
Size: 4394 2136
PflMI CCAn_nnn'nTGG
Cuts at: 435 961 1344 1836 2201 435
Size: 526 383 492 365 4764
Fragments arranged by size:
4764 526 492 383 365
PfoI T'CCnGG_A
Cuts at: 252 252
Size: 6530
PmeI GTTT'AAAC
Cuts at: 3529 3529
Size: 6530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5669 5701 5669
Size: 32 6498
PpuMI rG'GwC_Cy
Cuts at: 234 1348 1410 3208 234
Size: 1114 62 1798 3556
Fragments arranged by size:
3556 1798 1114 62
PshAI GACnn'nnGTC
Cuts at: 1123 1123
Size: 6530
PstI C_TGCA'G
Cuts at: 1169 1418 1479 1497 1515 1659 1757 2166 2941
Size: 249 61 18 18 144 98 409 775
Cuts at: 2941 2975 3577 4262 1169
Size: 34 602 685 3437
Fragments arranged by size:
3437 775 685 602 409 249 144 98
61 34 18 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6530
PvuII CAG'CTG
Cuts at: 215 1714 2071 3955 4315 215
Size: 1499 357 1884 360 2430
Fragments arranged by size:
2430 1884 1499 360 357
RsrII CG'GwC_CG
Cuts at: 4725 4725
Size: 6530
SacI G_AGCT'C
Cuts at: 2231 3544 2231
Size: 1313 5217
SalI G'TCGA_C
Cuts at: 3567 3567
Size: 6530
SanDI GG'GwC_CC
Cuts at: 1348 1348
Size: 6530
SapI GCTCTTCn'nnn_
Cuts at: 2396 3236 4553 4763 2396
Size: 840 1317 210 4163
Fragments arranged by size:
4163 1317 840 210
SbfI CC_TGCA'GG
Cuts at: 1418 1479 1497 1515 2975 3577 1418
Size: 61 18 18 1460 602 4371
Fragments arranged by size:
4371 1460 602 61 18 18
ScaI AGT'ACT
Cuts at: 6047 6047
Size: 6530
SexAI A'CCwGG_T
Cuts at: 1875 2177 2249 1875
Size: 302 72 6156
Fragments arranged by size:
6156 302 72
SfcI C'TryA_G
Cuts at: 33 627 740 1165 1414 1475 1493 1511 1655
Size: 594 113 425 249 61 18 18 144
Cuts at: 1655 1753 1937 2162 2937 2971 3573 4258 5251
Size: 98 184 225 775 34 602 685 993
Cuts at: 5251 5442 33
Size: 191 1121
Fragments arranged by size:
1121 993 775 685 602 594 425 249
225 191 184 144 113 98 61 34
18 18
SfoI GGC'GCC
Cuts at: 3962 4210 3962
Size: 248 6282
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6530
SmaI CCC'GGG
Cuts at: 2727 3143 3552 2727
Size: 416 409 5705
Fragments arranged by size:
5705 416 409
SmlI C'TyrA_G
Cuts at: 1245 2305 3685 4600 5092 5354 1245
Size: 1060 1380 915 492 262 2421
Fragments arranged by size:
2421 1380 1060 915 492 262
SnaBI TAC'GTA
Cuts at: 3522 3522
Size: 6530
SpeI A'CTAG_T
Cuts at: 67 1285 67
Size: 1218 5312
SphI G_CATG'C
Cuts at: 2103 3583 4083 2103
Size: 1480 500 4550
Fragments arranged by size:
4550 1480 500
SspI AAT'ATT
Cuts at: 546 769 546
Size: 223 6307
StuI AGG'CCT
Cuts at: 1809 2015 2152 2706 2791 1809
Size: 206 137 554 85 5548
Fragments arranged by size:
5548 554 206 137 85
StyI C'CwwG_G
Cuts at: 2951 3664 4018 2951
Size: 713 354 5463
Fragments arranged by size:
5463 713 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 1351 3357 4113 4246 4705 4914 697
Size: 654 2006 756 133 459 209 2313
Fragments arranged by size:
2313 2006 756 654 459 209 133
TatI w'GTAC_w
Cuts at: 623 3450 6045 623
Size: 2827 2595 1108
Fragments arranged by size:
2827 2595 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 1034 1066 2269 2301 6047 6079 305
Size: 32 697 32 1203 32 3746 32 756
Fragments arranged by size:
3746 1203 756 697 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4327 4327
Size: 6530
XbaI T'CTAG_A
Cuts at: 3561 3561
Size: 6530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1069 2342 4025 1069
Size: 1273 1683 3574
Fragments arranged by size:
3574 1683 1273
XmaI C'CCGG_G
Cuts at: 2725 3141 3550 2725
Size: 416 409 5705
Fragments arranged by size:
5705 416 409
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI ApaI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI PmlI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SrfI SwaI
XhoI XmnI ZraI