(Circular) (Six-base) MAPSORT of: pF1KSDA0865.seq Check: 2654 from: 1 to: 8711 pF1KSDA0865 8711 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1678 3632 4636 1678 Size: 1954 1004 5753 Fragments arranged by size: 5753 1954 1004 AccI GT'mk_AC Cuts at: 3459 5749 3459 Size: 2290 6421 Acc65I G'GTAC_C Cuts at: 5727 5727 Size: 8711 AclI AA'CG_TT Cuts at: 8551 8551 Size: 8711 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 289 385 694 1658 1786 1961 2070 3202 3613 Size: 96 309 964 128 175 109 1132 411 Cuts at: 3613 3722 5411 5659 6536 6968 7715 7983 289 Size: 109 1689 248 877 432 747 268 1017 Fragments arranged by size: 1689 1132 1017 964 877 747 432 411 309 268 248 175 128 109 109 96 AfeI AGC'GCT Cuts at: 1396 1396 Size: 8711 AflIII A'CryG_T Cuts at: 1716 3296 4547 6063 7167 8144 1716 Size: 1580 1251 1516 1104 977 2283 Fragments arranged by size: 2283 1580 1516 1251 1104 977 AgeI A'CCGG_T Cuts at: 1982 7099 1982 Size: 5117 3594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 5686 5686 Size: 8711 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1506 1538 2095 2127 3347 3379 1506 Size: 32 557 32 1220 32 6838 Fragments arranged by size: 6838 1220 557 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2205 3624 4892 5580 6016 7583 2205 Size: 1419 1268 688 436 1567 3333 Fragments arranged by size: 3333 1567 1419 1268 688 436 ApaI G_GGCC'C Cuts at: 4538 5009 4538 Size: 471 8240 ApaLI G'TGCA_C Cuts at: 1976 3225 7481 1976 Size: 1249 4256 3206 Fragments arranged by size: 4256 3206 1249 ApoI r'AATT_y Cuts at: 455 1841 2394 2491 2718 3016 5318 5367 5715 Size: 1386 553 97 227 298 2302 49 348 Cuts at: 5715 8064 8320 455 Size: 2349 256 846 Fragments arranged by size: 2349 2302 1386 846 553 348 298 256 227 97 49 AseI AT'TA_AT Cuts at: 20 20 Size: 8711 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8711 AvaI C'yCGr_G Cuts at: 399 1300 3749 4237 4263 4291 4405 4458 4864 Size: 901 2449 488 26 28 114 53 406 Cuts at: 4864 5166 5731 399 Size: 302 565 3379 Fragments arranged by size: 3379 2449 901 565 488 406 302 114 53 28 26 BamHI G'GATC_C Cuts at: 2986 5736 2986 Size: 2750 5961 BanI G'GyrC_C Cuts at: 1681 4258 4323 4962 5601 5727 6141 6389 6424 Size: 2577 65 639 639 126 414 248 35 Cuts at: 6424 1681 Size: 3968 Fragments arranged by size: 3968 2577 639 639 414 248 126 65 35 BanII G_rGCy'C Cuts at: 1112 1361 2070 4471 4538 4735 4922 5009 5150 Size: 249 709 2401 67 197 187 87 141 Cuts at: 5150 5597 5684 5725 6755 1112 Size: 447 87 41 1030 3068 Fragments arranged by size: 3068 2401 1030 709 447 249 197 187 141 87 87 67 41 BbeI G_GCGC'C Cuts at: 1685 4262 4327 4966 6145 6393 1685 Size: 2577 65 639 1179 248 4003 Fragments arranged by size: 4003 2577 1179 639 248 65 BbsI GAAGACnn'nnnn_ Cuts at: 503 503 Size: 8711 BbvCI CC'TCA_GC Cuts at: 5488 5488 Size: 8711 BciVI GTATCCnnnnn_n' Cuts at: 3349 5395 6609 7370 8711 3349 Size: 2046 1214 761 1341 3349 Fragments arranged by size: 3349 2046 1341 1214 761 BclI T'GATC_A Cuts at: 882 2948 6231 882 Size: 2066 3283 3362 Fragments arranged by size: 3362 3283 2066 BfrBI ATG'CAT Cuts at: 954 2014 954 Size: 1060 7651 BglI GCCn_nnn'nGGC Cuts at: 1690 3929 4267 4590 4766 4887 4999 5056 5071 Size: 2239 338 323 176 121 112 57 15 Cuts at: 5071 1690 Size: 5330 Fragments arranged by size: 5330 2239 338 323 176 121 112 57 15 BglII A'GATC_T Cuts at: 1412 3341 6226 8263 1412 Size: 1929 2885 2037 1860 Fragments arranged by size: 2885 2037 1929 1860 BlpI GC'TnA_GC Cuts at: 1351 3305 4201 5591 5823 1351 Size: 1954 896 1390 232 4239 Fragments arranged by size: 4239 1954 1390 896 232 Bme1580I G_kGCm'C Cuts at: 1980 3229 4379 4538 5009 6336 6429 7485 1980 Size: 1249 1150 159 471 1327 93 1056 3206 Fragments arranged by size: 3206 1327 1249 1150 1056 471 159 93 BmrI ACTGGGnnnn_n' Cuts at: 5923 6004 6339 8483 5923 Size: 81 335 2144 6151 Fragments arranged by size: 6151 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2193 7078 2193 Size: 4885 3826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 571 590 779 2182 3349 3454 4687 4711 5064 Size: 19 189 1403 1167 105 1233 24 353 Cuts at: 5064 5103 5556 571 Size: 39 453 3726 Fragments arranged by size: 3726 1403 1233 1167 453 353 189 105 39 24 19 Bpu10I CC'TnA_GC Cuts at: 1095 5488 1095 Size: 4393 4318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1085 4687 5276 5887 6766 7258 7556 1085 Size: 3602 589 611 879 492 298 2240 Fragments arranged by size: 3602 2240 879 611 589 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 708 857 2330 3950 4687 8415 708 Size: 149 1473 1620 737 3728 1004 Fragments arranged by size: 3728 1620 1473 1004 737 149 BsaAI yAC'GTr Cuts at: 3297 5703 6064 3297 Size: 2406 361 5944 Fragments arranged by size: 5944 2406 361 BsaBI GATnn'nnATC Cuts at: 6 6225 8262 6 Size: 6219 2037 455 Fragments arranged by size: 6219 2037 455 BsaHI Gr'CG_yC Cuts at: 508 997 1682 4259 4324 4963 5063 6142 6390 Size: 489 685 2577 65 639 100 1079 248 Cuts at: 6390 7092 8588 508 Size: 702 1496 631 Fragments arranged by size: 2577 1496 1079 702 685 639 631 489 248 100 65 BsaWI w'CCGG_w Cuts at: 1831 1982 5900 6421 7099 7373 7520 7931 1831 Size: 151 3918 521 678 274 147 411 2611 Fragments arranged by size: 3918 2611 678 521 411 274 151 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 464 494 563 593 752 782 823 853 982 Size: 30 69 30 159 30 41 30 129 Cuts at: 982 1012 1531 1561 2095 2125 2726 2756 3149 Size: 30 519 30 534 30 601 30 393 Cuts at: 3149 3179 3452 3482 3865 3895 4402 4417 4432 Size: 30 273 30 383 30 507 15 15 Cuts at: 4432 4447 464 Size: 15 4728 Fragments arranged by size: 4728 601 534 519 507 393 383 273 159 129 69 41 30 30 30 30 30 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 387 485 1003 1497 1526 3170 4157 4231 4438 Size: 98 518 494 29 1644 987 74 207 Cuts at: 4438 5187 5553 387 Size: 749 366 3545 Fragments arranged by size: 3545 1644 987 749 518 494 366 207 98 74 29 BseYI C'CCAG_C Cuts at: 1490 3877 4371 5113 5629 6136 6894 7471 7967 Size: 2387 494 742 516 507 758 577 496 Cuts at: 7967 1490 Size: 2234 Fragments arranged by size: 2387 2234 758 742 577 516 507 496 494 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4735 5566 3940 Size: 795 831 7085 Fragments arranged by size: 7085 831 795 BsiEI CG_ry'CG Cuts at: 104 3902 4594 4821 6253 6299 7507 104 Size: 3798 692 227 1432 46 1208 1308 Fragments arranged by size: 3798 1432 1308 1208 692 227 46 BsiHKAI G_wGCw'C Cuts at: 429 1361 1980 3229 5150 5684 5725 6503 6693 Size: 932 619 1249 1921 534 41 778 190 Cuts at: 6693 7485 429 Size: 792 1655 Fragments arranged by size: 1921 1655 1249 932 792 778 619 534 190 41 BsmI GAATG_Cn' Cuts at: 3738 3738 Size: 8711 BsmBI CGTCTCn'nnnn_ Cuts at: 399 4001 399 Size: 3602 5109 Bsp1286I G_dGCh'C Cuts at: 429 1112 1361 1980 2070 3229 4379 4471 4538 Size: 683 249 619 90 1159 1150 92 67 Cuts at: 4538 4735 4922 5009 5150 5597 5684 5725 6336 Size: 197 187 87 141 447 87 41 611 Cuts at: 6336 6429 6503 6693 6755 7485 429 Size: 93 74 190 62 730 1655 Fragments arranged by size: 1655 1159 1150 730 683 619 611 447 249 197 190 187 141 93 92 90 87 87 74 67 62 41 BspEI T'CCGG_A Cuts at: 7931 7931 Size: 8711 BspHI T'CATG_A Cuts at: 7887 7887 Size: 8711 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1296 1678 3592 3632 4586 4636 5761 5975 6277 Size: 382 1914 40 954 50 1125 214 302 Cuts at: 6277 6658 1296 Size: 381 3349 Fragments arranged by size: 3349 1914 1125 954 382 381 302 214 50 40 BsrBI CCG'CTC Cuts at: 50 1286 7003 7057 8541 8706 50 Size: 1236 5717 54 1484 165 55 Fragments arranged by size: 5717 1484 1236 165 55 54 BsrDI GCAATG_nn' Cuts at: 446 518 3243 3743 6623 446 Size: 72 2725 500 2880 2534 Fragments arranged by size: 2880 2725 2534 500 72 BsrFI r'CCGG_y Cuts at: 1982 3761 4589 5074 6709 6890 7099 1982 Size: 1779 828 485 1635 181 209 3594 Fragments arranged by size: 3594 1779 1635 828 485 209 181 BsrGI T'GTAC_A Cuts at: 2357 2580 4358 2357 Size: 223 1778 6710 Fragments arranged by size: 6710 1778 223 BssHII G'CGCG_C Cuts at: 4276 4307 4511 4895 4276 Size: 31 204 384 8092 Fragments arranged by size: 8092 384 204 31 BssSI C'ACGA_G Cuts at: 973 2563 6982 7340 973 Size: 1590 4419 358 2344 Fragments arranged by size: 4419 2344 1590 358 BstAPI GCAn_nnn'nTGC Cuts at: 2614 5580 6088 2614 Size: 2966 508 5237 Fragments arranged by size: 5237 2966 508 BstBI TT'CG_AA Cuts at: 110 7072 110 Size: 6962 1749 BstEII G'GTnAC_C Cuts at: 2620 2620 Size: 8711 BstYI r'GATC_y Cuts at: 1 1412 2986 3159 3341 5027 5576 5736 6203 Size: 1411 1574 173 182 1686 549 160 467 Cuts at: 6203 6226 6561 6807 7819 7905 7917 8263 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1686 1574 1411 1012 549 467 449 346 335 246 182 173 160 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1509 1509 Size: 8711 BtgI C'CryG_G Cuts at: 125 1481 2597 2817 3902 4000 4085 4253 4482 Size: 1356 1116 220 1085 98 85 168 229 Cuts at: 4482 4997 5192 5313 125 Size: 515 195 121 3523 Fragments arranged by size: 3523 1356 1116 1085 515 229 220 195 168 121 98 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3468 4475 6661 6842 7005 3468 Size: 1007 2186 181 163 5174 Fragments arranged by size: 5174 2186 1007 181 163 BtsI GCAGTG_nn' Cuts at: 691 3481 4309 5954 691 Size: 2790 828 1645 3448 Fragments arranged by size: 3448 2790 1645 828 DraI TTT'AAA Cuts at: 2283 3015 5710 2283 Size: 732 2695 5284 Fragments arranged by size: 5284 2695 732 DraIII CAC_nnn'GTG Cuts at: 975 3963 975 Size: 2988 5723 DrdI GACnn_nn'nnGTC Cuts at: 472 6417 7275 472 Size: 5945 858 1908 Fragments arranged by size: 5945 1908 858 EaeI y'GGCC_r Cuts at: 165 921 1890 2345 3899 4088 4100 4382 4591 Size: 756 969 455 1554 189 12 282 209 Cuts at: 4591 4818 5000 6296 6470 6861 6888 8233 165 Size: 227 182 1296 174 391 27 1345 643 Fragments arranged by size: 1554 1345 1296 969 756 643 455 391 282 227 209 189 182 174 27 12 EagI C'GGCC_G Cuts at: 3899 4591 4818 6296 3899 Size: 692 227 1478 6314 Fragments arranged by size: 6314 1478 692 227 EarI CTCTTCn'nnn_ Cuts at: 1100 1112 1549 1582 2468 5164 6734 6944 1100 Size: 12 437 33 886 2696 1570 210 2867 Fragments arranged by size: 2867 2696 1570 886 437 210 33 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2169 5074 6919 7229 7375 8521 2169 Size: 2905 1845 310 146 1146 2359 Fragments arranged by size: 2905 2359 1845 1146 310 146 EcoICRI GAG'CTC Cuts at: 1359 5148 5682 5723 1359 Size: 3789 534 41 4347 Fragments arranged by size: 4347 3789 534 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 289 385 571 590 694 779 1658 1786 1961 Size: 96 186 19 104 85 879 128 175 Cuts at: 1961 2070 2182 3202 3349 3454 3613 3722 4687 Size: 109 112 1020 147 105 159 109 965 Cuts at: 4687 4711 5064 5103 5411 5556 5659 6536 6968 Size: 24 353 39 308 145 103 877 432 Cuts at: 6968 7715 7983 289 Size: 747 268 1017 Fragments arranged by size: 1020 1017 965 879 877 747 432 353 308 268 186 175 159 147 145 128 112 109 109 105 104 103 96 85 39 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 367 586 2167 5664 367 Size: 219 1581 3497 3414 Fragments arranged by size: 3497 3414 1581 219 EcoO109I rG'GnC_Cy Cuts at: 407 3324 4032 4229 4534 4535 4889 5006 5654 Size: 2917 708 197 305 1 354 117 648 Cuts at: 5654 5850 407 Size: 196 3268 Fragments arranged by size: 3268 2917 708 648 354 305 197 196 117 1 EcoRI G'AATT_C Cuts at: 2394 5318 5715 2394 Size: 2924 397 5390 Fragments arranged by size: 5390 2924 397 EcoRV GAT'ATC Cuts at: 4223 4223 Size: 8711 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 370 2917 6730 370 Size: 2547 3813 2351 Fragments arranged by size: 3813 2547 2351 FspI TGC'GCA Cuts at: 6492 6492 Size: 8711 HaeII r_GCGC'y Cuts at: 1398 1685 4076 4262 4327 4804 4966 6145 6393 Size: 287 2391 186 65 477 162 1179 248 Cuts at: 6393 7415 8394 1398 Size: 1022 979 1715 Fragments arranged by size: 2391 1715 1179 1022 979 477 287 248 186 162 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 464 496 563 595 673 705 752 784 823 Size: 32 67 32 78 32 47 32 39 Cuts at: 823 855 982 1014 1066 1098 1918 1950 1999 Size: 32 127 32 52 32 820 32 49 Cuts at: 1999 2031 3149 3181 3373 3405 3452 3484 4152 Size: 32 1118 32 192 32 47 32 668 Cuts at: 4152 4184 4207 4239 5389 5421 6553 6583 6585 Size: 32 23 32 1150 32 1132 30 2 Cuts at: 6585 6615 464 Size: 30 2560 Fragments arranged by size: 2560 1150 1132 1118 820 668 192 127 78 67 52 49 47 47 39 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 23 2 HincII GTy'rAC Cuts at: 477 483 656 731 1449 2511 3460 5750 477 Size: 6 173 75 718 1062 949 2290 3438 Fragments arranged by size: 3438 2290 1062 949 718 173 75 6 HindIII A'AGCT_T Cuts at: 706 706 Size: 8711 HpaI GTT'AAC Cuts at: 731 1449 731 Size: 718 7993 KasI G'GCGC_C Cuts at: 1681 4258 4323 4962 6141 6389 1681 Size: 2577 65 639 1179 248 4003 Fragments arranged by size: 4003 2577 1179 639 248 65 KpnI G_GTAC'C Cuts at: 5731 5731 Size: 8711 MfeI C'AATT_G Cuts at: 1175 1175 Size: 8711 MluI A'CGCG_T Cuts at: 4547 8144 4547 Size: 3597 5114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1178 4667 7382 7566 1178 Size: 3489 2715 184 2323 Fragments arranged by size: 3489 2715 2323 184 MscI TGG'CCA Cuts at: 167 923 1892 2347 6472 167 Size: 756 969 455 4125 2406 Fragments arranged by size: 4125 2406 969 756 455 MslI CAynn'nnrTG Cuts at: 310 338 422 491 2836 4366 6700 6827 310 Size: 28 84 69 2345 1530 2334 127 2194 Fragments arranged by size: 2345 2334 2194 1530 127 84 69 28 MspA1I CmG'CkG Cuts at: 1494 1590 3445 4087 4205 4255 4737 5194 5487 Size: 96 1855 642 118 50 482 457 293 Cuts at: 5487 5822 6136 6496 7509 7754 1494 Size: 335 314 360 1013 245 2451 Fragments arranged by size: 2451 1855 1013 642 482 457 360 335 314 293 245 118 96 50 NaeI GCC'GGC Cuts at: 5076 6892 5076 Size: 1816 6895 NarI GG'CG_CC Cuts at: 1682 4259 4324 4963 6142 6390 1682 Size: 2577 65 639 1179 248 4003 Fragments arranged by size: 4003 2577 1179 639 248 65 NcoI C'CATG_G Cuts at: 125 1481 2597 5313 125 Size: 1356 1116 2716 3523 Fragments arranged by size: 3523 2716 1356 1116 NgoMIV G'CCGG_C Cuts at: 5074 6890 5074 Size: 1816 6895 NsiI A_TGCA'T Cuts at: 956 2016 956 Size: 1060 7651 NspI r_CATG'y Cuts at: 195 277 315 1720 1750 2433 3535 3822 5764 Size: 82 38 1405 30 683 1102 287 1942 Cuts at: 5764 6264 7171 195 Size: 500 907 1735 Fragments arranged by size: 1942 1735 1405 1102 907 683 500 287 82 38 30 PasI CC'CwG_GG Cuts at: 4540 5597 4540 Size: 1057 7654 PciI A'CATG_T Cuts at: 1716 7167 1716 Size: 5451 3260 PflMI CCAn_nnn'nTGG Cuts at: 2205 2205 Size: 8711 PfoI T'CCnGG_A Cuts at: 587 848 1580 2204 3326 4688 587 Size: 261 732 624 1122 1362 4610 Fragments arranged by size: 4610 1362 1122 732 624 261 PmeI GTTT'AAAC Cuts at: 5710 5710 Size: 8711 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2127 7850 7882 2095 Size: 32 5723 32 2924 Fragments arranged by size: 5723 2924 32 32 PpuMI rG'GwC_Cy Cuts at: 407 3324 4032 4229 407 Size: 2917 708 197 4889 Fragments arranged by size: 4889 2917 708 197 PshAI GACnn'nnGTC Cuts at: 3030 4621 3030 Size: 1591 7120 PsiI TTA'TAA Cuts at: 3580 3580 Size: 8711 PspOMI G'GGCC_C Cuts at: 4534 5005 4534 Size: 471 8240 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3772 3804 3772 Size: 32 8679 PstI C_TGCA'G Cuts at: 1675 3683 5014 5522 5758 6443 1675 Size: 2008 1331 508 236 685 3943 Fragments arranged by size: 3943 2008 1331 685 508 236 PvuI CG_AT'CG Cuts at: 104 104 Size: 8711 PvuII CAG'CTG Cuts at: 1494 3445 5487 6136 6496 1494 Size: 1951 2042 649 360 3709 Fragments arranged by size: 3709 2042 1951 649 360 RsrII CG'GwC_CG Cuts at: 6906 6906 Size: 8711 SacI G_AGCT'C Cuts at: 1361 5150 5684 5725 1361 Size: 3789 534 41 4347 Fragments arranged by size: 4347 3789 534 41 SacII CC_GC'GG Cuts at: 4088 4256 5195 4088 Size: 168 939 7604 Fragments arranged by size: 7604 939 168 SalI G'TCGA_C Cuts at: 3458 5748 3458 Size: 2290 6421 SanDI GG'GwC_CC Cuts at: 407 4032 4229 407 Size: 3625 197 4889 Fragments arranged by size: 4889 3625 197 SapI GCTCTTCn'nnn_ Cuts at: 1100 1582 6734 6944 1100 Size: 482 5152 210 2867 Fragments arranged by size: 5152 2867 482 210 SbfI CC_TGCA'GG Cuts at: 1675 5522 5758 1675 Size: 3847 236 4628 Fragments arranged by size: 4628 3847 236 ScaI AGT'ACT Cuts at: 889 4750 8228 889 Size: 3861 3478 1372 Fragments arranged by size: 3861 3478 1372 SfcI C'TryA_G Cuts at: 33 316 623 1671 3559 3679 4143 4884 5010 Size: 283 307 1048 1888 120 464 741 126 Cuts at: 5010 5103 5518 5754 6439 7432 7623 33 Size: 93 415 236 685 993 191 1121 Fragments arranged by size: 1888 1121 1048 993 741 685 464 415 307 283 236 191 126 120 93 SfoI GGC'GCC Cuts at: 1683 4260 4325 4964 6143 6391 1683 Size: 2577 65 639 1179 248 4003 Fragments arranged by size: 4003 2577 1179 639 248 65 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8711 SmaI CCC'GGG Cuts at: 3751 4239 4265 4293 4407 4460 4866 5733 3751 Size: 488 26 28 114 53 406 867 6729 Fragments arranged by size: 6729 867 488 406 114 53 28 26 SmlI C'TyrA_G Cuts at: 1100 4702 5291 5866 6781 7273 7535 1100 Size: 3602 589 575 915 492 262 2276 Fragments arranged by size: 3602 2276 915 589 575 492 262 SnaBI TAC'GTA Cuts at: 5703 5703 Size: 8711 SpeI A'CTAG_T Cuts at: 67 2932 67 Size: 2865 5846 SphI G_CATG'C Cuts at: 195 277 2433 3535 5764 6264 195 Size: 82 2156 1102 2229 500 2642 Fragments arranged by size: 2642 2229 2156 1102 500 82 SrfI GCCC'GGGC Cuts at: 4460 4866 4460 Size: 406 8305 SspI AAT'ATT Cuts at: 2132 2278 2453 2132 Size: 146 175 8390 Fragments arranged by size: 8390 175 146 StuI AGG'CCT Cuts at: 5124 5124 Size: 8711 StyI C'CwwG_G Cuts at: 125 162 1481 2139 2597 4967 5313 5845 6199 Size: 37 1319 658 458 2370 346 532 354 Cuts at: 6199 125 Size: 2637 Fragments arranged by size: 2637 2370 1319 658 532 458 354 346 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 4991 6294 6427 6886 7095 4991 Size: 1303 133 459 209 6607 Fragments arranged by size: 6607 1303 459 209 133 TatI w'GTAC_w Cuts at: 887 2357 2580 2771 4358 4748 8226 887 Size: 1470 223 191 1587 390 3478 1372 Fragments arranged by size: 3478 1587 1470 1372 390 223 191 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1531 1563 3865 3897 4417 4449 8228 8260 1531 Size: 32 2302 32 520 32 3779 32 1982 Fragments arranged by size: 3779 2302 1982 520 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 6508 6508 Size: 8711 XbaI T'CTAG_A Cuts at: 3093 5742 3093 Size: 2649 6062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 595 2659 3925 4110 6206 595 Size: 2064 1266 185 2096 3100 Fragments arranged by size: 3100 2096 2064 1266 185 XmaI C'CCGG_G Cuts at: 3749 4237 4263 4291 4405 4458 4864 5731 3749 Size: 488 26 28 114 53 406 867 6729 Fragments arranged by size: 6729 867 488 406 114 53 28 26 XmnI GAAnn'nnTTC Cuts at: 353 2110 2477 3343 353 Size: 1757 367 866 5721 Fragments arranged by size: 5721 1757 866 367 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI AleI AscI AvrII BaeI BcgI BmgBI BmtI BsiWI BstXI BstZ17I ClaI CspCI FseI FspAI NdeI NheI NotI NruI PacI PmlI PspXI SexAI SfiI SgrAI SwaI XhoI ZraI