Restriction map for
pF1KSDA0875
(Circular) (Six-base) MAPSORT of: pF1KSDA0875.seq Check: 4109 from: 1 to: 5002
pF1KSDA0875 5002 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 307 853 1690 307
Size: 546 837 3619
Fragments arranged by size:
3619 837 546
AccI GT'mk_AC
Cuts at: 143 2038 143
Size: 1895 3107
Acc65I G'GTAC_C
Cuts at: 2016 2016
Size: 5002
AclI AA'CG_TT
Cuts at: 4842 4842
Size: 5002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 667 964 2825 3257 4006 4274 501
Size: 166 297 1861 432 749 268 1229
Fragments arranged by size:
1861 1229 749 432 297 268 166
AflII C'TTAA_G
Cuts at: 670 670
Size: 5002
AflIII A'CryG_T
Cuts at: 2352 3458 4435 2352
Size: 1106 977 2919
Fragments arranged by size:
2919 1106 977
AgeI A'CCGG_T
Cuts at: 3390 3390
Size: 5002
AhdI GACnn_n'nnGTC
Cuts at: 702 702
Size: 5002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1057 1256 1057
Size: 199 4803
AlwNI CAG_nnn'CTG
Cuts at: 299 909 1347 2305 3874 299
Size: 610 438 958 1569 1427
Fragments arranged by size:
1569 1427 958 610 438
ApaLI G'TGCA_C
Cuts at: 3772 3772
Size: 5002
ApoI r'AATT_y
Cuts at: 629 2004 4355 4611 629
Size: 1375 2351 256 1020
Fragments arranged by size:
2351 1375 1020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5002
AvaI C'yCGr_G
Cuts at: 1488 2020 1488
Size: 532 4470
BamHI G'GATC_C
Cuts at: 1703 2025 1703
Size: 322 4680
BanI G'GyrC_C
Cuts at: 162 1121 2016 2430 2678 2713 162
Size: 959 895 414 248 35 2451
Fragments arranged by size:
2451 959 895 414 248 35
BanII G_rGCy'C
Cuts at: 1625 2014 3044 1625
Size: 389 1030 3583
Fragments arranged by size:
3583 1030 389
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4754
BbvCI CC'TCA_GC
Cuts at: 205 205
Size: 5002
BciVI GTATCCnnnnn_n'
Cuts at: 586 1925 2898 3661 5002 586
Size: 1339 973 763 1341 586
Fragments arranged by size:
1341 1339 973 763 586
BclI T'GATC_A
Cuts at: 2520 2520
Size: 5002
BfrBI ATG'CAT
Cuts at: 996 996
Size: 5002
BglI GCCn_nnn'nGGC
Cuts at: 859 859
Size: 5002
BglII A'GATC_T
Cuts at: 656 1923 2515 4554 656
Size: 1267 592 2039 1104
Fragments arranged by size:
2039 1267 1104 592
BlpI GC'TnA_GC
Cuts at: 2112 2112
Size: 5002
Bme1580I G_kGCm'C
Cuts at: 2625 2718 3776 2625
Size: 93 1058 3851
Fragments arranged by size:
3851 1058 93
BmgBI CAC'GTC
Cuts at: 1848 1848
Size: 5002
BmrI ACTGGGnnnn_n'
Cuts at: 2212 2293 2628 4774 2212
Size: 81 335 2146 2440
Fragments arranged by size:
2440 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1794 3367 1794
Size: 1573 3429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 276 880 1102 1450 1924 1948 259
Size: 17 604 222 348 474 24 3313
Fragments arranged by size:
3313 604 474 348 222 24 17
Bpu10I CC'TnA_GC
Cuts at: 205 205
Size: 5002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1811 2176 3055 3549 3847 1811
Size: 365 879 494 298 2966
Fragments arranged by size:
2966 879 494 365 298
BsaI GGTCTCn'nnnn_
Cuts at: 4706 4706
Size: 5002
BsaAI yAC'GTr
Cuts at: 1236 1992 2353 1236
Size: 756 361 3885
Fragments arranged by size:
3885 756 361
BsaBI GATnn'nnATC
Cuts at: 6 1367 2514 4553 6
Size: 1361 1147 2039 455
Fragments arranged by size:
2039 1361 1147 455
BsaHI Gr'CG_yC
Cuts at: 2431 2679 3383 4879 2431
Size: 248 704 1496 2554
Fragments arranged by size:
2554 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1706 1910 2189 2710 3390 3664 3811 4222 1706
Size: 204 279 521 680 274 147 411 2486
Fragments arranged by size:
2486 680 521 411 279 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1897 1927 1897
Size: 30 4972
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 1393 1396 1570 193
Size: 1200 3 174 3625
Fragments arranged by size:
3625 1200 174 3
BseYI C'CCAG_C
Cuts at: 442 844 1670 2425 3183 3762 4258 442
Size: 402 826 755 758 579 496 1186
Fragments arranged by size:
1186 826 758 755 579 496 402
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 1540 1969 1414
Size: 126 429 4447
Fragments arranged by size:
4447 429 126
BsiEI CG_ry'CG
Cuts at: 104 272 285 2542 2588 3798 104
Size: 168 13 2257 46 1210 1308
Fragments arranged by size:
2257 1308 1210 168 46 13
BsiHKAI G_wGCw'C
Cuts at: 500 1538 2014 2792 2982 3776 500
Size: 1038 476 778 190 794 1726
Fragments arranged by size:
1726 1038 794 778 476 190
BsmI GAATG_Cn'
Cuts at: 1216 1216
Size: 5002
Bsp1286I G_dGCh'C
Cuts at: 500 1538 1625 2014 2625 2718 2792 2982 3044
Size: 1038 87 389 611 93 74 190 62
Cuts at: 3044 3776 500
Size: 732 1726
Fragments arranged by size:
1726 1038 732 611 389 190 93 87
74 62
BspEI T'CCGG_A
Cuts at: 1706 4222 1706
Size: 2516 2486
BspHI T'CATG_A
Cuts at: 4178 4178
Size: 5002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 307 650 853 1690 2050 2264 2566 2947
Size: 77 343 203 837 360 214 302 381
Cuts at: 2947 230
Size: 2285
Fragments arranged by size:
2285 837 381 360 343 302 214 203
77
BsrBI CCG'CTC
Cuts at: 50 3292 3346 4832 4997 50
Size: 3242 54 1486 165 55
Fragments arranged by size:
3242 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1382 2912 1382
Size: 1530 3472
BsrFI r'CCGG_y
Cuts at: 165 302 2998 3179 3390 165
Size: 137 2696 181 211 1777
Fragments arranged by size:
2696 1777 211 181 137
BssSI C'ACGA_G
Cuts at: 1696 3271 3631 1696
Size: 1575 360 3067
Fragments arranged by size:
3067 1575 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2377 2377
Size: 5002
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5002
BstXI CCAn_nnnn'nTGG
Cuts at: 882 882
Size: 5002
BstYI r'GATC_y
Cuts at: 1 656 1362 1703 1907 1923 2025 2492 2515
Size: 655 706 341 204 16 102 467 23
Cuts at: 2515 2850 3096 4110 4196 4208 4554 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 706 655 467 449 346 341 335
246 204 102 86 23 16 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5002
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 166 2950 3131 3294 166
Size: 2784 181 163 1874
Fragments arranged by size:
2784 1874 181 163
BtsI GCAGTG_nn'
Cuts at: 2243 2243
Size: 5002
DraI TTT'AAA
Cuts at: 1999 1999
Size: 5002
DrdI GACnn_nn'nnGTC
Cuts at: 2706 3566 2706
Size: 860 4142
EaeI y'GGCC_r
Cuts at: 269 282 1227 1441 1737 2585 2759 3150 3177
Size: 13 945 214 296 848 174 391 27
Cuts at: 3177 4524 269
Size: 1347 747
Fragments arranged by size:
1347 945 848 747 391 296 214 174
27 13
EagI C'GGCC_G
Cuts at: 269 282 2585 269
Size: 13 2303 2686
Fragments arranged by size:
2686 2303 13
EarI CTCTTCn'nnn_
Cuts at: 413 3023 3233 413
Size: 2610 210 2182
Fragments arranged by size:
2610 2182 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 3208 3520 3666 4812 190
Size: 3018 312 146 1146 380
Fragments arranged by size:
3018 1146 380 312 146
EcoICRI GAG'CTC
Cuts at: 2012 2012
Size: 5002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 276 501 667 880 964 1102 1450 1924
Size: 17 225 166 213 84 138 348 474
Cuts at: 1924 1948 2825 3257 4006 4274 259
Size: 24 877 432 749 268 987
Fragments arranged by size:
987 877 749 474 432 348 268 225
213 166 138 84 24 17
EcoO109I rG'GnC_Cy
Cuts at: 1674 2139 1674
Size: 465 4537
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 617 1435 3019 504
Size: 113 818 1584 2487
Fragments arranged by size:
2487 1584 818 113
FspI TGC'GCA
Cuts at: 2781 2781
Size: 5002
HaeII r_GCGC'y
Cuts at: 172 2434 2682 3706 4685 172
Size: 2262 248 1024 979 489
Fragments arranged by size:
2262 1024 979 489 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1044 1076 1899 1931 2842 2872 2874
Size: 32 324 32 823 32 911 30 2
Cuts at: 2874 2904 688
Size: 30 2786
Fragments arranged by size:
2786 911 823 324 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 144 219 1092 2039 144
Size: 75 873 947 3107
Fragments arranged by size:
3107 947 873 75
HindIII A'AGCT_T
Cuts at: 599 599
Size: 5002
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4754
KpnI G_GTAC'C
Cuts at: 2020 2020
Size: 5002
MfeI C'AATT_G
Cuts at: 408 1055 408
Size: 647 4355
MluI A'CGCG_T
Cuts at: 4435 4435
Size: 5002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 779 3673 3857 395
Size: 384 2894 184 1540
Fragments arranged by size:
2894 1540 384 184
MscI TGG'CCA
Cuts at: 1443 2761 1443
Size: 1318 3684
MslI CAynn'nnrTG
Cuts at: 880 1601 1684 2989 3116 880
Size: 721 83 1305 127 2766
Fragments arranged by size:
2766 1305 721 127 83
MspA1I CmG'CkG
Cuts at: 209 332 1197 2111 2425 2785 3800 4045 209
Size: 123 865 914 314 360 1015 245 1166
Fragments arranged by size:
1166 1015 914 865 360 314 245 123
NaeI GCC'GGC
Cuts at: 167 304 3181 167
Size: 137 2877 1988
Fragments arranged by size:
2877 1988 137
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4754
NcoI C'CATG_G
Cuts at: 125 125
Size: 5002
NgoMIV G'CCGG_C
Cuts at: 165 302 3179 165
Size: 137 2877 1988
Fragments arranged by size:
2877 1988 137
NruI TCG'CGA
Cuts at: 3363 3363
Size: 5002
NsiI A_TGCA'T
Cuts at: 998 998
Size: 5002
NspI r_CATG'y
Cuts at: 1038 2053 2553 3462 1038
Size: 1015 500 909 2578
Fragments arranged by size:
2578 1015 909 500
PciI A'CATG_T
Cuts at: 3458 3458
Size: 5002
PflMI CCAn_nnn'nTGG
Cuts at: 909 909
Size: 5002
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5002
PmlI CAC'GTG
Cuts at: 1236 1236
Size: 5002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4141 4173 4141
Size: 32 4970
PpuMI rG'GwC_Cy
Cuts at: 1674 1674
Size: 5002
PstI C_TGCA'G
Cuts at: 1245 2047 2732 1245
Size: 802 685 3515
Fragments arranged by size:
3515 802 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5002
PvuII CAG'CTG
Cuts at: 209 1197 2425 2785 209
Size: 988 1228 360 2426
Fragments arranged by size:
2426 1228 988 360
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5002
SacI G_AGCT'C
Cuts at: 2014 2014
Size: 5002
SalI G'TCGA_C
Cuts at: 142 2037 142
Size: 1895 3107
SanDI GG'GwC_CC
Cuts at: 1674 1674
Size: 5002
SapI GCTCTTCn'nnn_
Cuts at: 3023 3233 3023
Size: 210 4792
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5002
ScaI AGT'ACT
Cuts at: 739 1219 4519 739
Size: 480 3300 1222
Fragments arranged by size:
3300 1222 480
SexAI A'CCwGG_T
Cuts at: 1388 1514 1388
Size: 126 4876
SfcI C'TryA_G
Cuts at: 33 387 1241 2043 2728 3723 3914 33
Size: 354 854 802 685 995 191 1121
Fragments arranged by size:
1121 995 854 802 685 354 191
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4754
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5002
SmaI CCC'GGG
Cuts at: 1490 2022 1490
Size: 532 4470
SmlI C'TyrA_G
Cuts at: 670 1826 2155 3070 3564 3826 670
Size: 1156 329 915 494 262 1846
Fragments arranged by size:
1846 1156 915 494 329 262
SnaBI TAC'GTA
Cuts at: 1992 1992
Size: 5002
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5002
SphI G_CATG'C
Cuts at: 2053 2553 2053
Size: 500 4502
SspI AAT'ATT
Cuts at: 1956 1956
Size: 5002
StuI AGG'CCT
Cuts at: 676 676
Size: 5002
StyI C'CwwG_G
Cuts at: 125 1125 2134 2488 125
Size: 1000 1009 354 2639
Fragments arranged by size:
2639 1009 1000 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2583 2716 3175 3386 2583
Size: 133 459 211 4199
Fragments arranged by size:
4199 459 211 133
TatI w'GTAC_w
Cuts at: 242 737 1217 4517 242
Size: 495 480 3300 727
Fragments arranged by size:
3300 727 495 480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4519 4551 4519
Size: 32 4970
Tth111I GACn'n_nGTC
Cuts at: 403 2797 403
Size: 2394 2608
XbaI T'CTAG_A
Cuts at: 1530 2031 1530
Size: 501 4501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1686 2495 1686
Size: 809 4193
XmaI C'CCGG_G
Cuts at: 1488 2020 1488
Size: 532 4470
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BbsI BcgI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SrfI SwaI XhoI XmnI ZraI