Restriction map for
pF1KSDA0876
(Circular) (Six-base) MAPSORT of: pF1KSDA0876.seq Check: 8958 from: 1 to: 6427
pF1KSDA0876 6427 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 2839 3375 336
Size: 2503 536 3388
Fragments arranged by size:
3388 2503 536
AccI GT'mk_AC
Cuts at: 3463 3463
Size: 6427
Acc65I G'GTAC_C
Cuts at: 3441 3441
Size: 6427
AclI AA'CG_TT
Cuts at: 6267 6267
Size: 6427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 882 898 1240 4250 4682 5431 5699 882
Size: 16 342 3010 432 749 268 1610
Fragments arranged by size:
3010 1610 749 432 342 268 16
AfeI AGC'GCT
Cuts at: 1111 1322 1111
Size: 211 6216
AflIII A'CryG_T
Cuts at: 1082 1757 2476 3777 4883 5860 1082
Size: 675 719 1301 1106 977 1649
Fragments arranged by size:
1649 1301 1106 977 719 675
AgeI A'CCGG_T
Cuts at: 4815 4815
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2444 2444
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 1407 1606 2155 3730 5299 1407
Size: 199 549 1575 1569 2535
Fragments arranged by size:
2535 1575 1569 549 199
ApaI G_GGCC'C
Cuts at: 1600 1600
Size: 6427
ApaLI G'TGCA_C
Cuts at: 1044 5197 1044
Size: 4153 2274
ApoI r'AATT_y
Cuts at: 196 925 3429 5780 6036 196
Size: 729 2504 2351 256 587
Fragments arranged by size:
2504 2351 729 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6427
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6427
AvaI C'yCGr_G
Cuts at: 811 1105 1183 1208 1378 1892 2452 2599 2656
Size: 294 78 25 170 514 560 147 57
Cuts at: 2656 3276 3445 811
Size: 620 169 3793
Fragments arranged by size:
3793 620 560 514 294 170 169 147
78 57 25
AvrII C'CTAG_G
Cuts at: 2886 2886
Size: 6427
BamHI G'GATC_C
Cuts at: 890 2570 3450 890
Size: 1680 880 3867
Fragments arranged by size:
3867 1680 880
BanI G'GyrC_C
Cuts at: 145 333 458 622 1635 1662 1719 1911 1958
Size: 188 125 164 1013 27 57 192 47
Cuts at: 1958 2232 2716 2947 3255 3316 3441 3855 4103
Size: 274 484 231 308 61 125 414 248
Cuts at: 4103 4138 145
Size: 35 2434
Fragments arranged by size:
2434 1013 484 414 308 274 248 231
192 188 164 125 125 61 57 47
35 27
BanII G_rGCy'C
Cuts at: 246 820 1195 1586 1600 1627 2171 3080 3269
Size: 574 375 391 14 27 544 909 189
Cuts at: 3269 3408 3439 4469 246
Size: 139 31 1030 2204
Fragments arranged by size:
2204 1030 909 574 544 391 375 189
139 31 27 14
BbeI G_GCGC'C
Cuts at: 149 337 1962 3859 4107 149
Size: 188 1625 1897 248 2469
Fragments arranged by size:
2469 1897 1625 248 188
BbsI GAAGACnn'nnnn_
Cuts at: 679 1289 2299 679
Size: 610 1010 4807
Fragments arranged by size:
4807 1010 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 612 646 612
Size: 34 6393
BciVI GTATCCnnnnn_n'
Cuts at: 2734 4323 5086 6427 2734
Size: 1589 763 1341 2734
Fragments arranged by size:
2734 1589 1341 763
BclI T'GATC_A
Cuts at: 932 3945 932
Size: 3013 3414
BglI GCCn_nnn'nGGC
Cuts at: 250 365 250
Size: 115 6312
BglII A'GATC_T
Cuts at: 1070 3940 5979 1070
Size: 2870 2039 1518
Fragments arranged by size:
2870 2039 1518
BlpI GC'TnA_GC
Cuts at: 1189 3537 1189
Size: 2348 4079
Bme1580I G_kGCm'C
Cuts at: 1048 1600 1655 1667 3258 3319 4050 4143 5201
Size: 552 55 12 1591 61 731 93 1058
Cuts at: 5201 1048
Size: 2274
Fragments arranged by size:
2274 1591 1058 731 552 93 61 55
12
BmgBI CAC'GTC
Cuts at: 321 561 1083 2477 2753 321
Size: 240 522 1394 276 3995
Fragments arranged by size:
3995 1394 522 276 240
BmrI ACTGGGnnnn_n'
Cuts at: 561 1661 3637 3718 4053 6199 561
Size: 1100 1976 81 335 2146 789
Fragments arranged by size:
2146 1976 1100 789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1209 2763 4792 1209
Size: 1554 2029 2844
Fragments arranged by size:
2844 2029 1554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1218 1600 3223 805
Size: 413 382 1623 4009
Fragments arranged by size:
4009 1623 413 382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2004 2100 2821 3601 4480 4974 5272 2004
Size: 96 721 780 879 494 298 3159
Fragments arranged by size:
3159 879 780 721 494 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 1247 1876 2826 6131 1247
Size: 629 950 3305 1543
Fragments arranged by size:
3305 1543 950 629
BsaAI yAC'GTr
Cuts at: 219 1049 1716 1758 2577 2763 3360 3417 3778
Size: 830 667 42 819 186 597 57 361
Cuts at: 3778 219
Size: 2868
Fragments arranged by size:
2868 830 819 667 597 361 186 57
42
BsaBI GATnn'nnATC
Cuts at: 6 1942 3939 5978 6
Size: 1936 1997 2039 455
Fragments arranged by size:
2039 1997 1936 455
BsaHI Gr'CG_yC
Cuts at: 146 334 833 1959 2075 3856 4104 4808 6304
Size: 188 499 1126 116 1781 248 704 1496
Cuts at: 6304 146
Size: 269
Fragments arranged by size:
1781 1496 1126 704 499 269 248 188
116
BsaWI w'CCGG_w
Cuts at: 587 1247 3080 3614 4135 4815 5089 5236 5647
Size: 660 1833 534 521 680 274 147 411
Cuts at: 5647 587
Size: 1367
Fragments arranged by size:
1833 1367 680 660 534 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 730 760 1546 1576 1744 1774 2974
Size: 30 99 30 786 30 168 30 1200
Cuts at: 2974 3004 3067 3097 601
Size: 30 63 30 3931
Fragments arranged by size:
3931 1200 786 168 99 63 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 1345 1369 1396 1399 1402 1405 1408 1567
Size: 123 24 27 3 3 3 3 159
Cuts at: 1567 1633 1687 2044 2549 3111 3220 3223 3298
Size: 66 54 357 505 562 109 3 75
Cuts at: 3298 1222
Size: 4351
Fragments arranged by size:
4351 562 505 357 159 123 109 75
66 54 27 24 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 157 1312 1336 1360 1593 3045 3167 3850 4608
Size: 1155 24 24 233 1452 122 683 758
Cuts at: 4608 5187 5683 157
Size: 579 496 901
Fragments arranged by size:
1452 1155 901 758 683 579 496 233
122 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1874 2169 3409 712
Size: 1162 295 1240 3730
Fragments arranged by size:
3730 1240 1162 295
BsiEI CG_ry'CG
Cuts at: 104 1419 1729 1978 2302 3967 4013 5223 104
Size: 1315 310 249 324 1665 46 1210 1308
Fragments arranged by size:
1665 1315 1308 1210 324 310 249 46
BsiHKAI G_wGCw'C
Cuts at: 773 820 1048 1195 1757 1784 2734 2744 2792
Size: 47 228 147 562 27 950 10 48
Cuts at: 2792 3080 3250 3439 4217 4407 5201 773
Size: 288 170 189 778 190 794 1999
Fragments arranged by size:
1999 950 794 778 562 288 228 190
189 170 147 48 47 27 10
Bsp1286I G_dGCh'C
Cuts at: 246 773 820 1048 1195 1586 1600 1627 1655
Size: 527 47 228 147 391 14 27 28
Cuts at: 1655 1667 1757 1784 2171 2734 2744 2792 3080
Size: 12 90 27 387 563 10 48 288
Cuts at: 3080 3250 3258 3269 3319 3408 3439 4050 4143
Size: 170 8 11 50 89 31 611 93
Cuts at: 4143 4217 4407 4469 5201 246
Size: 74 190 62 732 1472
Fragments arranged by size:
1472 732 611 563 527 391 387 288
228 190 170 147 93 90 89 74
62 50 48 47 31 28 27 27
14 12 11 10 8
BspEI T'CCGG_A
Cuts at: 587 5647 587
Size: 5060 1367
BspHI T'CATG_A
Cuts at: 170 929 5603 170
Size: 759 4674 994
Fragments arranged by size:
4674 994 759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 734 1229 1861 2412 2537 2776 2839 2971
Size: 398 495 632 551 125 239 63 132
Cuts at: 2971 3375 3475 3689 3991 4372 336
Size: 404 100 214 302 381 2391
Fragments arranged by size:
2391 632 551 495 404 398 381 302
239 214 132 125 100 63
BsrBI CCG'CTC
Cuts at: 50 789 1485 2304 3230 4717 4771 6257 6422
Size: 739 696 819 926 1487 54 1486 165
Cuts at: 6422 50
Size: 55
Fragments arranged by size:
1487 1486 926 819 739 696 165 55
54
BsrDI GCAATG_nn'
Cuts at: 4337 4337
Size: 6427
BsrFI r'CCGG_y
Cuts at: 958 1632 1955 1973 3395 4423 4604 4815 958
Size: 674 323 18 1422 1028 181 211 2570
Fragments arranged by size:
2570 1422 1028 674 323 211 181 18
BsrGI T'GTAC_A
Cuts at: 710 710
Size: 6427
BssHII G'CGCG_C
Cuts at: 2158 2605 2158
Size: 447 5980
BssSI C'ACGA_G
Cuts at: 3024 4696 5056 3024
Size: 1672 360 4395
Fragments arranged by size:
4395 1672 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1407 2155 2414 3802 1407
Size: 748 259 1388 4032
Fragments arranged by size:
4032 1388 748 259
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6427
BstXI CCAn_nnnn'nTGG
Cuts at: 408 408
Size: 6427
BstYI r'GATC_y
Cuts at: 1 890 1070 2570 3450 3917 3940 4275 4521
Size: 889 180 1500 880 467 23 335 246
Cuts at: 4521 5535 5621 5633 5979 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1500 1014 889 880 467 449 346 335
246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1658 2872 1658
Size: 1214 5213
BtgI C'CryG_G
Cuts at: 125 141 188 292 454 1076 1166 1613 1723
Size: 16 47 104 162 622 90 447 110
Cuts at: 1723 1826 3329 125
Size: 103 1503 3223
Fragments arranged by size:
3223 1503 622 447 162 110 104 103
90 47 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2574 4375 4556 4719 2574
Size: 1801 181 163 4282
Fragments arranged by size:
4282 1801 181 163
BtsI GCAGTG_nn'
Cuts at: 1405 1869 2107 2742 3668 1405
Size: 464 238 635 926 4164
Fragments arranged by size:
4164 926 635 464 238
DraI TTT'AAA
Cuts at: 201 3424 201
Size: 3223 3204
DraIII CAC_nnn'GTG
Cuts at: 1043 2637 1043
Size: 1594 4833
DrdI GACnn_nn'nnGTC
Cuts at: 360 4131 4991 360
Size: 3771 860 1796
Fragments arranged by size:
3771 1796 860
EaeI y'GGCC_r
Cuts at: 260 428 794 1034 1416 1462 1726 1975 2117
Size: 168 366 240 382 46 264 249 142
Cuts at: 2117 2264 4010 4184 4575 4602 5949 260
Size: 147 1746 174 391 27 1347 738
Fragments arranged by size:
1746 1347 738 391 382 366 264 249
240 174 168 147 142 46 27
EagI C'GGCC_G
Cuts at: 1416 1726 1975 4010 1416
Size: 310 249 2035 3833
Fragments arranged by size:
3833 2035 310 249
EarI CTCTTCn'nnn_
Cuts at: 376 1433 1489 1945 2197 4448 4658 376
Size: 1057 56 456 252 2251 210 2145
Fragments arranged by size:
2251 2145 1057 456 252 210 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3360 4633 4945 5091 6237 3360
Size: 1273 312 146 1146 3550
Fragments arranged by size:
3550 1273 1146 312 146
EcoICRI GAG'CTC
Cuts at: 818 1193 3078 3437 818
Size: 375 1885 359 3808
Fragments arranged by size:
3808 1885 375 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 882 898 1218 1240 1600 3223 4250 4682
Size: 77 16 320 22 360 1623 1027 432
Cuts at: 4682 5431 5699 805
Size: 749 268 1533
Fragments arranged by size:
1623 1533 1027 749 432 360 320 268
77 22 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1330 2251 2890 3103 3205 3382 1330
Size: 921 639 213 102 177 4375
Fragments arranged by size:
4375 921 639 213 177 102
EcoO109I rG'GnC_Cy
Cuts at: 1137 1603 2211 3049 3164 3564 1137
Size: 466 608 838 115 400 4000
Fragments arranged by size:
4000 838 608 466 400 115
EcoRI G'AATT_C
Cuts at: 925 3429 925
Size: 2504 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 696 1515 1758 4444 696
Size: 819 243 2686 2679
Fragments arranged by size:
2686 2679 819 243
FseI GG_CCGG'CC
Cuts at: 1977 3399 1977
Size: 1422 5005
FspI TGC'GCA
Cuts at: 984 1093 4206 984
Size: 109 3113 3205
Fragments arranged by size:
3205 3113 109
FspAI rTGC'GCAy
Cuts at: 1093 1093
Size: 6427
HaeII r_GCGC'y
Cuts at: 149 337 783 1113 1174 1324 1561 1962 2631
Size: 188 446 330 61 150 237 401 669
Cuts at: 2631 2651 3306 3859 4107 5131 6110 149
Size: 20 655 553 248 1024 979 466
Fragments arranged by size:
1024 979 669 655 553 466 446 401
330 248 237 188 150 61 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 1744 1776 2020 2052 2291 2323 2974
Size: 32 982 32 244 32 239 32 651
Cuts at: 2974 3006 3148 3180 4267 4297 4299 4329 730
Size: 32 142 32 1087 30 2 30 2828
Fragments arranged by size:
2828 1087 982 651 244 239 142 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1374 3464 1374
Size: 2090 4337
KasI G'GCGC_C
Cuts at: 145 333 1958 3855 4103 145
Size: 188 1625 1897 248 2469
Fragments arranged by size:
2469 1897 1625 248 188
KpnI G_GTAC'C
Cuts at: 3445 3445
Size: 6427
MluI A'CGCG_T
Cuts at: 5860 5860
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2889 5098 5282 1825
Size: 1064 2209 184 2970
Fragments arranged by size:
2970 2209 1064 184
MscI TGG'CCA
Cuts at: 262 430 796 1036 4186 262
Size: 168 366 240 3150 2503
Fragments arranged by size:
3150 2503 366 240 168
MslI CAynn'nnrTG
Cuts at: 406 1081 1705 4414 4541 406
Size: 675 624 2709 127 2292
Fragments arranged by size:
2709 2292 675 624 127
MspA1I CmG'CkG
Cuts at: 161 294 456 539 1521 1725 2088 2152 2352
Size: 133 162 83 982 204 363 64 200
Cuts at: 2352 2664 2933 3045 3171 3536 3850 4210 5225
Size: 312 269 112 126 365 314 360 1015
Cuts at: 5225 5470 161
Size: 245 1118
Fragments arranged by size:
1118 1015 982 365 363 360 314 312
269 245 204 200 162 133 126 112
83 64
NaeI GCC'GGC
Cuts at: 960 1634 1957 1975 3397 4606 960
Size: 674 323 18 1422 1209 2781
Fragments arranged by size:
2781 1422 1209 674 323 18
NarI GG'CG_CC
Cuts at: 146 334 1959 3856 4104 146
Size: 188 1625 1897 248 2469
Fragments arranged by size:
2469 1897 1625 248 188
NcoI C'CATG_G
Cuts at: 125 188 1076 1613 1826 125
Size: 63 888 537 213 4726
Fragments arranged by size:
4726 888 537 213 63
NgoMIV G'CCGG_C
Cuts at: 958 1632 1955 1973 3395 4604 958
Size: 674 323 18 1422 1209 2781
Fragments arranged by size:
2781 1422 1209 674 323 18
NruI TCG'CGA
Cuts at: 4788 4788
Size: 6427
NspI r_CATG'y
Cuts at: 669 3478 3978 4887 669
Size: 2809 500 909 2209
Fragments arranged by size:
2809 2209 909 500
PasI CC'CwG_GG
Cuts at: 1297 1968 1297
Size: 671 5756
PciI A'CATG_T
Cuts at: 4883 4883
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 348 1968 2637 348
Size: 1620 669 4138
Fragments arranged by size:
4138 1620 669
PfoI T'CCnGG_A
Cuts at: 596 1195 596
Size: 599 5828
PmeI GTTT'AAAC
Cuts at: 3424 3424
Size: 6427
PmlI CAC'GTG
Cuts at: 1049 1758 2577 2763 1049
Size: 709 819 186 4713
Fragments arranged by size:
4713 819 709 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2157 5566 5598 2125
Size: 32 3409 32 2954
Fragments arranged by size:
3409 2954 32 32
PpuMI rG'GwC_Cy
Cuts at: 1137 3049 3164 1137
Size: 1912 115 4400
Fragments arranged by size:
4400 1912 115
PspOMI G'GGCC_C
Cuts at: 1596 1596
Size: 6427
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 634 666 634
Size: 32 6395
PstI C_TGCA'G
Cuts at: 1103 1611 2102 2423 2549 3386 3472 4157 1103
Size: 508 491 321 126 837 86 685 3373
Fragments arranged by size:
3373 837 685 508 491 321 126 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6427
PvuII CAG'CTG
Cuts at: 161 1521 3045 3171 3850 4210 161
Size: 1360 1524 126 679 360 2378
Fragments arranged by size:
2378 1524 1360 679 360 126
RsrII CG'GwC_CG
Cuts at: 4620 4620
Size: 6427
SacI G_AGCT'C
Cuts at: 820 1195 3080 3439 820
Size: 375 1885 359 3808
Fragments arranged by size:
3808 1885 375 359
SacII CC_GC'GG
Cuts at: 295 457 1726 295
Size: 162 1269 4996
Fragments arranged by size:
4996 1269 162
SalI G'TCGA_C
Cuts at: 3462 3462
Size: 6427
SanDI GG'GwC_CC
Cuts at: 3049 3164 3049
Size: 115 6312
SapI GCTCTTCn'nnn_
Cuts at: 1489 1945 2197 4448 4658 1489
Size: 456 252 2251 210 3258
Fragments arranged by size:
3258 2251 456 252 210
SbfI CC_TGCA'GG
Cuts at: 2423 3386 3472 2423
Size: 963 86 5378
Fragments arranged by size:
5378 963 86
ScaI AGT'ACT
Cuts at: 445 5944 445
Size: 5499 928
SexAI A'CCwGG_T
Cuts at: 3143 3143
Size: 6427
SfcI C'TryA_G
Cuts at: 33 1099 1607 2098 2215 2419 2545 2994 3382
Size: 1066 508 491 117 204 126 449 388
Cuts at: 3382 3468 4153 5148 5339 33
Size: 86 685 995 191 1121
Fragments arranged by size:
1121 1066 995 685 508 491 449 388
204 191 126 117 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 365 365
Size: 6427
SfoI GGC'GCC
Cuts at: 147 335 1960 3857 4105 147
Size: 188 1625 1897 248 2469
Fragments arranged by size:
2469 1897 1625 248 188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6427
SmaI CCC'GGG
Cuts at: 813 1210 1894 3447 813
Size: 397 684 1553 3793
Fragments arranged by size:
3793 1553 684 397
SmlI C'TyrA_G
Cuts at: 2019 2079 2836 3580 4495 4989 5251 2019
Size: 60 757 744 915 494 262 3195
Fragments arranged by size:
3195 915 757 744 494 262 60
SnaBI TAC'GTA
Cuts at: 3417 3417
Size: 6427
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6427
SphI G_CATG'C
Cuts at: 3478 3978 3478
Size: 500 5927
SrfI GCCC'GGGC
Cuts at: 1894 1894
Size: 6427
StuI AGG'CCT
Cuts at: 1774 2017 1774
Size: 243 6184
StyI C'CwwG_G
Cuts at: 125 188 1076 1469 1613 1826 2886 3290 3559
Size: 63 888 393 144 213 1060 404 269
Cuts at: 3559 3913 125
Size: 354 2639
Fragments arranged by size:
2639 1060 888 404 393 354 269 213
144 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2250 4008 4141 4600 4811 2250
Size: 1758 133 459 211 3866
Fragments arranged by size:
3866 1758 459 211 133
TatI w'GTAC_w
Cuts at: 380 443 656 710 2684 2924 5942 380
Size: 63 213 54 1974 240 3018 865
Fragments arranged by size:
3018 1974 865 240 213 63 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 601 633 1546 1578 3067 3099 5944 5976 601
Size: 32 913 32 1489 32 2845 32 1052
Fragments arranged by size:
2845 1489 1052 913 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1063 3037 4222 1063
Size: 1974 1185 3268
Fragments arranged by size:
3268 1974 1185
XbaI T'CTAG_A
Cuts at: 3456 3456
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 600 1713 1935 3920 345
Size: 255 1113 222 1985 2852
Fragments arranged by size:
2852 1985 1113 255 222
XmaI C'CCGG_G
Cuts at: 811 1208 1892 3445 811
Size: 397 684 1553 3793
Fragments arranged by size:
3793 1553 684 397
XmnI GAAnn'nnTTC
Cuts at: 1498 1498
Size: 6427
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BstEII BstZ17I
ClaI CspCI EcoRV HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PshAI PsiI PspXI SgrAI SspI
SwaI XhoI ZraI