Restriction map for
pF1KSDA0880
(Circular) (Six-base) MAPSORT of: pF1KSDA0880.seq Check: 9126 from: 1 to: 5264
pF1KSDA0880 5264 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1095 1095
Size: 5264
AatII G_ACGT'C
Cuts at: 385 385
Size: 5264
AccI GT'mk_AC
Cuts at: 2302 2302
Size: 5264
Acc65I G'GTAC_C
Cuts at: 156 165 2280 156
Size: 9 2115 3140
Fragments arranged by size:
3140 2115 9
AclI AA'CG_TT
Cuts at: 5104 5104
Size: 5264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1476 3089 3521 4268 4536 1033
Size: 443 1613 432 747 268 1761
Fragments arranged by size:
1761 1613 747 443 432 268
AfeI AGC'GCT
Cuts at: 361 361
Size: 5264
AflIII A'CryG_T
Cuts at: 2616 3720 4697 2616
Size: 1104 977 3183
Fragments arranged by size:
3183 1104 977
AgeI A'CCGG_T
Cuts at: 2072 3652 2072
Size: 1580 3684
AleI CACnn'nnGTG
Cuts at: 1651 2002 1651
Size: 351 4913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 475 883 1783 1797 475
Size: 408 900 14 3942
Fragments arranged by size:
3942 900 408 14
AlwNI CAG_nnn'CTG
Cuts at: 681 717 2110 2569 4136 681
Size: 36 1393 459 1567 1809
Fragments arranged by size:
1809 1567 1393 459 36
ApaI G_GGCC'C
Cuts at: 1441 2016 1441
Size: 575 4689
ApaLI G'TGCA_C
Cuts at: 460 2005 4034 460
Size: 1545 2029 1690
Fragments arranged by size:
2029 1690 1545
ApoI r'AATT_y
Cuts at: 2268 4617 4873 2268
Size: 2349 256 2659
Fragments arranged by size:
2659 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
AvaI C'yCGr_G
Cuts at: 805 2246 2284 805
Size: 1441 38 3785
Fragments arranged by size:
3785 1441 38
BamHI G'GATC_C
Cuts at: 809 1777 2289 809
Size: 968 512 3784
Fragments arranged by size:
3784 968 512
BanI G'GyrC_C
Cuts at: 156 165 735 946 1453 1809 2280 2694 2942
Size: 9 570 211 507 356 471 414 248
Cuts at: 2942 2977 156
Size: 35 2443
Fragments arranged by size:
2443 570 507 471 414 356 248 211
35 9
BanII G_rGCy'C
Cuts at: 340 1126 1441 1840 2016 2278 3308 340
Size: 786 315 399 176 262 1030 2296
Fragments arranged by size:
2296 1030 786 399 315 262 176
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5016
BbsI GAAGACnn'nnnn_
Cuts at: 1194 1910 1194
Size: 716 4548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 757 791 1971 2005 757
Size: 34 1180 34 4016
Fragments arranged by size:
4016 1180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3162 3923 5264 3162
Size: 761 1341 3162
Fragments arranged by size:
3162 1341 761
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5264
BglI GCCn_nnn'nGGC
Cuts at: 1679 1679
Size: 5264
BglII A'GATC_T
Cuts at: 2779 4816 2779
Size: 2037 3227
BlpI GC'TnA_GC
Cuts at: 2376 2376
Size: 5264
Bme1580I G_kGCm'C
Cuts at: 199 464 740 1441 1458 1563 1814 2009 2016
Size: 265 276 701 17 105 251 195 7
Cuts at: 2016 2889 2982 4038 199
Size: 873 93 1056 1425
Fragments arranged by size:
1425 1056 873 701 276 265 251 195
105 93 17 7
BmgBI CAC'GTC
Cuts at: 243 243
Size: 5264
BmrI ACTGGGnnnn_n'
Cuts at: 2019 2476 2557 2892 5036 2019
Size: 457 81 335 2144 2247
Fragments arranged by size:
2247 2144 457 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1620 3631 1620
Size: 2011 3253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 357 1339 1506 1594 2071 233
Size: 124 982 167 88 477 3426
Fragments arranged by size:
3426 982 477 167 124 88
Bpu10I CC'TnA_GC
Cuts at: 2017 2017
Size: 5264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 1671 2440 3319 3811 4109 642
Size: 1029 769 879 492 298 1797
Fragments arranged by size:
1797 1029 879 769 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4968 4968
Size: 5264
BsaAI yAC'GTr
Cuts at: 2256 2617 2256
Size: 361 4903
BsaBI GATnn'nnATC
Cuts at: 6 2778 4815 6
Size: 2772 2037 455
Fragments arranged by size:
2772 2037 455
BsaHI Gr'CG_yC
Cuts at: 382 1412 2028 2695 2943 3645 5141 382
Size: 1030 616 667 248 702 1496 505
Fragments arranged by size:
1496 1030 702 667 616 505 248
BsaWI w'CCGG_w
Cuts at: 2072 2453 2974 3652 3926 4073 4484 2072
Size: 381 521 678 274 147 411 2852
Fragments arranged by size:
2852 678 521 411 381 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1312 1342 1852 1882 1312
Size: 30 510 30 4694
Fragments arranged by size:
4694 510 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1904 1904
Size: 5264
BseYI C'CCAG_C
Cuts at: 627 674 720 855 957 1467 1642 1691 1963
Size: 47 46 135 102 510 175 49 272
Cuts at: 1963 2689 3447 4024 4520 627
Size: 726 758 577 496 1371
Fragments arranged by size:
1371 758 726 577 510 496 272 175
135 102 49 47 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1815 1001
Size: 814 4450
BsiEI CG_ry'CG
Cuts at: 104 2806 2852 4060 104
Size: 2702 46 1208 1308
Fragments arranged by size:
2702 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 340 464 2009 2278 3056 3246 4038 340
Size: 124 1545 269 778 190 792 1566
Fragments arranged by size:
1566 1545 792 778 269 190 124
Bsp1286I G_dGCh'C
Cuts at: 199 340 464 740 1126 1441 1458 1563 1814
Size: 141 124 276 386 315 17 105 251
Cuts at: 1814 1840 2009 2016 2278 2889 2982 3056 3246
Size: 26 169 7 262 611 93 74 190
Cuts at: 3246 3308 4038 199
Size: 62 730 1425
Fragments arranged by size:
1425 730 611 386 315 276 262 251
190 169 141 124 105 93 74 62
26 17 7
BspEI T'CCGG_A
Cuts at: 4484 4484
Size: 5264
BspHI T'CATG_A
Cuts at: 314 518 4440 314
Size: 204 3922 1138
Fragments arranged by size:
3922 1138 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1095 1364 2069 2314 2528 2830 3211 1095
Size: 269 705 245 214 302 381 3148
Fragments arranged by size:
3148 705 381 302 269 245 214
BsrBI CCG'CTC
Cuts at: 50 1503 3556 3610 5094 5259 50
Size: 1453 2053 54 1484 165 55
Fragments arranged by size:
2053 1484 1453 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3176 3176
Size: 5264
BsrFI r'CCGG_y
Cuts at: 976 2072 3262 3443 3652 976
Size: 1096 1190 181 209 2588
Fragments arranged by size:
2588 1190 1096 209 181
BssSI C'ACGA_G
Cuts at: 1649 3535 3893 1649
Size: 1886 358 3020
Fragments arranged by size:
3020 1886 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2208 2641 2208
Size: 433 4831
BstBI TT'CG_AA
Cuts at: 110 3625 110
Size: 3515 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1317 1317
Size: 5264
BstYI r'GATC_y
Cuts at: 1 809 1777 2182 2289 2756 2779 3114 3360
Size: 808 968 405 107 467 23 335 246
Cuts at: 3360 4372 4458 4470 4816 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 968 808 467 449 405 346 335
246 107 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 236 572 1942 2143 236
Size: 336 1370 201 3357
Fragments arranged by size:
3357 1370 336 201
BtgI C'CryG_G
Cuts at: 125 1280 1977 125
Size: 1155 697 3412
Fragments arranged by size:
3412 1155 697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 954 3214 3395 3558 954
Size: 2260 181 163 2660
Fragments arranged by size:
2660 2260 181 163
BtsI GCAGTG_nn'
Cuts at: 827 989 2507 827
Size: 162 1518 3584
Fragments arranged by size:
3584 1518 162
ClaI AT'CG_AT
Cuts at: 767 767
Size: 5264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1676 1711 1676
Size: 35 5229
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5264
DrdI GACnn_nn'nnGTC
Cuts at: 2970 3828 2970
Size: 858 4406
EaeI y'GGCC_r
Cuts at: 247 1295 2849 3023 3414 3441 4786 247
Size: 1048 1554 174 391 27 1345 725
Fragments arranged by size:
1554 1345 1048 725 391 174 27
EagI C'GGCC_G
Cuts at: 2849 2849
Size: 5264
EarI CTCTTCn'nnn_
Cuts at: 1492 1510 3287 3497 1492
Size: 18 1777 210 3259
Fragments arranged by size:
3259 1777 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3472 3782 3928 5074 3472
Size: 310 146 1146 3662
Fragments arranged by size:
3662 1146 310 146
EcoICRI GAG'CTC
Cuts at: 338 2276 338
Size: 1938 3326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 357 1033 1339 1476 1506 1594 2071 3089
Size: 124 676 306 137 30 88 477 1018
Cuts at: 3089 3521 4268 4536 233
Size: 432 747 268 961
Fragments arranged by size:
1018 961 747 676 477 432 306 268
137 124 88 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1261 1261
Size: 5264
EcoO109I rG'GnC_Cy
Cuts at: 130 930 1438 2013 2403 130
Size: 800 508 575 390 2991
Fragments arranged by size:
2991 800 575 508 390
EcoRI G'AATT_C
Cuts at: 2268 2268
Size: 5264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 5264
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5264
HaeII r_GCGC'y
Cuts at: 363 1326 1988 2698 2946 3968 4947 363
Size: 963 662 710 248 1022 979 680
Fragments arranged by size:
1022 979 963 710 680 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1096 1128 2167 2199 3106 3136 3138 3168 1096
Size: 32 1039 32 907 30 2 30 3192
Fragments arranged by size:
3192 1039 907 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2303 2303
Size: 5264
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5016
KpnI G_GTAC'C
Cuts at: 160 169 2284 160
Size: 9 2115 3140
Fragments arranged by size:
3140 2115 9
MfeI C'AATT_G
Cuts at: 2206 2206
Size: 5264
MluI A'CGCG_T
Cuts at: 4697 4697
Size: 5264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3935 4119 3935
Size: 184 5080
MscI TGG'CCA
Cuts at: 1297 3025 1297
Size: 1728 3536
MslI CAynn'nnrTG
Cuts at: 683 703 1402 1651 2002 3253 3380 683
Size: 20 699 249 351 1251 127 2567
Fragments arranged by size:
2567 1251 699 351 249 127 20
MspA1I CmG'CkG
Cuts at: 148 303 1745 2375 2689 3049 4062 4307 148
Size: 155 1442 630 314 360 1013 245 1105
Fragments arranged by size:
1442 1105 1013 630 360 314 245 155
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 5264
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5016
NcoI C'CATG_G
Cuts at: 125 1280 125
Size: 1155 4109
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 5264
NspI r_CATG'y
Cuts at: 2317 2817 3724 2317
Size: 500 907 3857
Fragments arranged by size:
3857 907 500
PasI CC'CwG_GG
Cuts at: 1126 1208 1567 1126
Size: 82 359 4823
Fragments arranged by size:
4823 359 82
PciI A'CATG_T
Cuts at: 3720 3720
Size: 5264
PflMI CCAn_nnn'nTGG
Cuts at: 907 1806 907
Size: 899 4365
PfoI T'CCnGG_A
Cuts at: 1316 1700 1316
Size: 384 4880
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 5232
PpuMI rG'GwC_Cy
Cuts at: 130 930 130
Size: 800 4464
PspOMI G'GGCC_C
Cuts at: 1437 2012 1437
Size: 575 4689
PstI C_TGCA'G
Cuts at: 302 1223 1744 2311 2996 302
Size: 921 521 567 685 2570
Fragments arranged by size:
2570 921 685 567 521
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5264
PvuII CAG'CTG
Cuts at: 303 1745 2689 3049 303
Size: 1442 944 360 2518
Fragments arranged by size:
2518 1442 944 360
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5264
SacI G_AGCT'C
Cuts at: 340 2278 340
Size: 1938 3326
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5264
SanDI GG'GwC_CC
Cuts at: 130 130
Size: 5264
SapI GCTCTTCn'nnn_
Cuts at: 1510 3287 3497 1510
Size: 1777 210 3277
Fragments arranged by size:
3277 1777 210
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5264
ScaI AGT'ACT
Cuts at: 4781 4781
Size: 5264
SfcI C'TryA_G
Cuts at: 33 298 1219 1740 2307 2992 3985 4176 33
Size: 265 921 521 567 685 993 191 1121
Fragments arranged by size:
1121 993 921 685 567 521 265 191
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5016
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
SmaI CCC'GGG
Cuts at: 2286 2286
Size: 5264
SmlI C'TyrA_G
Cuts at: 657 1686 2419 3334 3826 4088 657
Size: 1029 733 915 492 262 1833
Fragments arranged by size:
1833 1029 915 733 492 262
SnaBI TAC'GTA
Cuts at: 2256 2256
Size: 5264
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5264
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4764
StuI AGG'CCT
Cuts at: 842 1600 842
Size: 758 4506
StyI C'CwwG_G
Cuts at: 125 1019 1031 1280 2398 2752 125
Size: 894 12 249 1118 354 2637
Fragments arranged by size:
2637 1118 894 354 249 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 930 934 1374 1396 1683 1873 2847 2980 3439
Size: 4 440 22 287 190 974 133 459
Cuts at: 3439 3648 930
Size: 209 2546
Fragments arranged by size:
2546 974 459 440 287 209 190 133
22 4
TatI w'GTAC_w
Cuts at: 4779 4779
Size: 5264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1852 1884 4781 4813 1852
Size: 32 2897 32 2303
Fragments arranged by size:
2897 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5264
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 348 1245 1650 2759 189
Size: 159 897 405 1109 2694
Fragments arranged by size:
2694 1109 897 405 159
XmaI C'CCGG_G
Cuts at: 2284 2284
Size: 5264
XmnI GAAnn'nnTTC
Cuts at: 361 361
Size: 5264
ZraI GAC'GTC
Cuts at: 383 383
Size: 5264
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbvCI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SrfI SspI SwaI
XhoI