Restriction map for
pF1KSDA0881
(Circular) (Six-base) MAPSORT of: pF1KSDA0881.seq Check: 1231 from: 1 to: 6284
pF1KSDA0881 6284 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 666 666
Size: 6284
AatII G_ACGT'C
Cuts at: 749 749
Size: 6284
AccI GT'mk_AC
Cuts at: 251 899 3322 251
Size: 648 2423 3213
Fragments arranged by size:
3213 2423 648
Acc65I G'GTAC_C
Cuts at: 3300 3300
Size: 6284
AclI AA'CG_TT
Cuts at: 6124 6124
Size: 6284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 478 517 895 973 1328 1927 2921 4109
Size: 303 39 378 78 355 599 994 1188
Cuts at: 4109 4541 5288 5556 175
Size: 432 747 268 903
Fragments arranged by size:
1188 994 903 747 599 432 378 355
303 268 78 39
AflII C'TTAA_G
Cuts at: 2479 2479
Size: 6284
AflIII A'CryG_T
Cuts at: 2395 3636 4740 5717 2395
Size: 1241 1104 977 2962
Fragments arranged by size:
2962 1241 1104 977
AgeI A'CCGG_T
Cuts at: 4672 4672
Size: 6284
AhdI GACnn_n'nnGTC
Cuts at: 750 750
Size: 6284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1598 2092 2336 1598
Size: 494 244 5546
Fragments arranged by size:
5546 494 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 343 375 1798 1830 2584 2616 343
Size: 32 1423 32 754 32 4011
Fragments arranged by size:
4011 1423 754 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 463 540 1644 2106 2571 2634 3141 3589 5156
Size: 77 1104 462 465 63 507 448 1567
Cuts at: 5156 463
Size: 1591
Fragments arranged by size:
1591 1567 1104 507 465 462 448 77
63
ApaI G_GGCC'C
Cuts at: 3077 3187 3212 3077
Size: 110 25 6149
Fragments arranged by size:
6149 110 25
ApaLI G'TGCA_C
Cuts at: 478 1688 2156 5054 478
Size: 1210 468 2898 1708
Fragments arranged by size:
2898 1708 1210 468
ApoI r'AATT_y
Cuts at: 891 3288 5637 5893 891
Size: 2397 2349 256 1282
Fragments arranged by size:
2397 2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6284
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6284
AvaI C'yCGr_G
Cuts at: 260 697 1168 1313 3156 3205 3304 260
Size: 437 471 145 1843 49 99 3240
Fragments arranged by size:
3240 1843 471 437 145 99 49
BamHI G'GATC_C
Cuts at: 3309 3309
Size: 6284
BanI G'GyrC_C
Cuts at: 654 676 693 1083 1099 1760 3201 3300 3714
Size: 22 17 390 16 661 1441 99 414
Cuts at: 3714 3962 3997 654
Size: 248 35 2941
Fragments arranged by size:
2941 1441 661 414 390 248 99 35
22 17 16
BanII G_rGCy'C
Cuts at: 179 457 1136 1390 1919 2240 3077 3111 3187
Size: 278 679 254 529 321 837 34 76
Cuts at: 3187 3212 3298 4328 179
Size: 25 86 1030 2135
Fragments arranged by size:
2135 1030 837 679 529 321 278 254
86 76 34 25
BbeI G_GCGC'C
Cuts at: 3718 3966 3718
Size: 248 6036
BbsI GAAGACnn'nnnn_
Cuts at: 901 901
Size: 6284
BbvCI CC'TCA_GC
Cuts at: 1663 1663
Size: 6284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3213 3247 3213
Size: 34 6250
BciVI GTATCCnnnnn_n'
Cuts at: 4182 4943 6284 4182
Size: 761 1341 4182
Fragments arranged by size:
4182 1341 761
BclI T'GATC_A
Cuts at: 3804 3804
Size: 6284
BglI GCCn_nnn'nGGC
Cuts at: 3144 3144
Size: 6284
BglII A'GATC_T
Cuts at: 3799 5836 3799
Size: 2037 4247
BlpI GC'TnA_GC
Cuts at: 173 456 1600 2234 2462 2557 3396 173
Size: 283 1144 634 228 95 839 3061
Fragments arranged by size:
3061 1144 839 634 283 228 95
Bme1580I G_kGCm'C
Cuts at: 482 679 1190 1692 2160 2739 2979 3077 3187
Size: 197 511 502 468 579 240 98 110
Cuts at: 3187 3206 3212 3909 4002 5058 482
Size: 19 6 697 93 1056 1708
Fragments arranged by size:
1708 1056 697 579 511 502 468 240
197 110 98 93 19 6
BmgBI CAC'GTC
Cuts at: 2396 2396
Size: 6284
BmrI ACTGGGnnnn_n'
Cuts at: 3496 3577 3912 6056 3496
Size: 81 335 2144 3724
Fragments arranged by size:
3724 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1596 4651 1596
Size: 3055 3229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1194 1426 1672 1885 1903 1969 2194 2215
Size: 350 232 246 213 18 66 225 21
Cuts at: 2215 2299 2464 2656 2731 2764 2856 3002 3003
Size: 84 165 192 75 33 92 146 1
Cuts at: 3003 844
Size: 4125
Fragments arranged by size:
4125 350 246 232 225 213 192 165
146 92 84 75 66 33 21 18
1
Bpu10I CC'TnA_GC
Cuts at: 1663 3214 1663
Size: 1551 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 1729 2473 2833 3460 4339 4831 5129 908
Size: 821 744 360 627 879 492 298 2063
Fragments arranged by size:
2063 879 821 744 627 492 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 1508 2440 2578 5988 1508
Size: 932 138 3410 1804
Fragments arranged by size:
3410 1804 932 138
BsaAI yAC'GTr
Cuts at: 3276 3637 3276
Size: 361 5923
BsaBI GATnn'nnATC
Cuts at: 6 2668 3798 5835 6
Size: 2662 1130 2037 455
Fragments arranged by size:
2662 2037 1130 455
BsaHI Gr'CG_yC
Cuts at: 746 3027 3715 3963 4665 6161 746
Size: 2281 688 248 702 1496 869
Fragments arranged by size:
2281 1496 869 702 688 248
BsaWI w'CCGG_w
Cuts at: 269 887 3473 3994 4672 4946 5093 5504 269
Size: 618 2586 521 678 274 147 411 1049
Fragments arranged by size:
2586 1049 678 618 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 356 373 386 1798 1828 2584 2614 343
Size: 13 17 13 1412 30 756 30 4013
Fragments arranged by size:
4013 1412 756 30 30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1129 1138 1279 1282 1285 1288 1291 1300 1303
Size: 9 141 3 3 3 3 9 3
Cuts at: 1303 1306 1693 1819 1927 2017 2776 2987 1129
Size: 3 387 126 108 90 759 211 4426
Fragments arranged by size:
4426 759 387 211 141 126 108 90
9 9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 133 1189 1432 1458 1930 2265 2862 2940 3069
Size: 1056 243 26 472 335 597 78 129
Cuts at: 3069 3134 3709 4467 5044 5540 133
Size: 65 575 758 577 496 877
Fragments arranged by size:
1056 877 758 597 577 575 496 472
335 243 129 78 65 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 2239 535
Size: 1704 4580
BsiEI CG_ry'CG
Cuts at: 104 3826 3872 5080 104
Size: 3722 46 1208 1308
Fragments arranged by size:
3722 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 422 482 860 971 1346 1685 1692 1919
Size: 243 60 378 111 375 339 7 227
Cuts at: 1919 2160 2240 2436 3298 4076 4266 5058 179
Size: 241 80 196 862 778 190 792 1405
Fragments arranged by size:
1405 862 792 778 378 375 339 243
241 227 196 190 111 80 60 7
BsmI GAATG_Cn'
Cuts at: 816 816
Size: 6284
BsmBI CGTCTCn'nnnn_
Cuts at: 2386 3041 2386
Size: 655 5629
Bsp1286I G_dGCh'C
Cuts at: 179 422 457 482 679 860 971 1136 1190
Size: 243 35 25 197 181 111 165 54
Cuts at: 1190 1346 1390 1685 1692 1919 2160 2240 2436
Size: 156 44 295 7 227 241 80 196
Cuts at: 2436 2739 2979 3077 3111 3187 3206 3212 3298
Size: 303 240 98 34 76 19 6 86
Cuts at: 3298 3909 4002 4076 4266 4328 5058 179
Size: 611 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 611 303 295 243 241 240
227 197 196 190 181 165 156 111
98 93 86 80 76 74 62 54
44 35 34 25 19 7 6
BspEI T'CCGG_A
Cuts at: 269 887 5504 269
Size: 618 4617 1049
Fragments arranged by size:
4617 1049 618
BspHI T'CATG_A
Cuts at: 5460 5460
Size: 6284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 666 775 1052 2114 3334 3548 3850 4231 666
Size: 109 277 1062 1220 214 302 381 2719
Fragments arranged by size:
2719 1220 1062 381 302 277 214 109
BsrBI CCG'CTC
Cuts at: 50 798 978 1604 1809 2817 4576 4630 6114
Size: 748 180 626 205 1008 1759 54 1484
Cuts at: 6114 6279 50
Size: 165 55
Fragments arranged by size:
1759 1484 1008 748 626 205 180 165
55 54
BsrDI GCAATG_nn'
Cuts at: 833 4196 833
Size: 3363 2921
BsrFI r'CCGG_y
Cuts at: 1376 4282 4463 4672 1376
Size: 2906 181 209 2988
Fragments arranged by size:
2988 2906 209 181
BssSI C'ACGA_G
Cuts at: 1787 2176 2689 4555 4913 1787
Size: 389 513 1866 358 3158
Fragments arranged by size:
3158 1866 513 389 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1792 2307 3661 1792
Size: 515 1354 4415
Fragments arranged by size:
4415 1354 515
BstBI TT'CG_AA
Cuts at: 110 4645 110
Size: 4535 1749
BstYI r'GATC_y
Cuts at: 1 1067 1559 2402 2669 3309 3776 3799 4134
Size: 1066 492 843 267 640 467 23 335
Cuts at: 4134 4380 5392 5478 5490 5836 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1066 1012 843 640 492 467 449 346
335 267 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 252 252
Size: 6284
Bsu36I CC'TnA_GG
Cuts at: 412 412
Size: 6284
BtgI C'CryG_G
Cuts at: 231 522 713 2988 3099 3197 231
Size: 291 191 2275 111 98 3318
Fragments arranged by size:
3318 2275 291 191 111 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 829 4234 4415 4578 829
Size: 3405 181 163 2535
Fragments arranged by size:
3405 2535 181 163
BtsI GCAGTG_nn'
Cuts at: 1996 2056 2158 2737 3171 3177 3527 1996
Size: 60 102 579 434 6 350 4753
Fragments arranged by size:
4753 579 434 350 102 60 6
DraI TTT'AAA
Cuts at: 3283 3283
Size: 6284
DraIII CAC_nnn'GTG
Cuts at: 1185 3088 3100 1185
Size: 1903 12 4369
Fragments arranged by size:
4369 1903 12
DrdI GACnn_nn'nnGTC
Cuts at: 3990 4848 3990
Size: 858 5426
EaeI y'GGCC_r
Cuts at: 228 287 710 979 1322 3869 4043 4434 4461
Size: 59 423 269 343 2547 174 391 27
Cuts at: 4461 5806 228
Size: 1345 706
Fragments arranged by size:
2547 1345 706 423 391 343 269 174
59 27
EagI C'GGCC_G
Cuts at: 3869 3869
Size: 6284
EarI CTCTTCn'nnn_
Cuts at: 184 211 1115 1256 1277 1485 2489 2753 4307
Size: 27 904 141 21 208 1004 264 1554
Cuts at: 4307 4517 184
Size: 210 1951
Fragments arranged by size:
1951 1554 1004 904 264 210 208 141
27 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2539 3119 4492 4802 4948 6094 2014
Size: 525 580 1373 310 146 1146 2204
Fragments arranged by size:
2204 1373 1146 580 525 310 146
EcoICRI GAG'CTC
Cuts at: 177 1917 2238 3296 177
Size: 1740 321 1058 3165
Fragments arranged by size:
3165 1740 1058 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 478 517 844 895 973 1194 1328 1426
Size: 303 39 327 51 78 221 134 98
Cuts at: 1426 1672 1885 1903 1927 1969 2194 2215 2299
Size: 246 213 18 24 42 225 21 84
Cuts at: 2299 2464 2656 2731 2764 2856 2921 3002 3003
Size: 165 192 75 33 92 65 81 1
Cuts at: 3003 4109 4541 5288 5556 175
Size: 1106 432 747 268 903
Fragments arranged by size:
1106 903 747 432 327 303 268 246
225 221 213 192 165 134 98 92
84 81 78 75 65 51 42 39
33 24 21 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 496 1864 2098 3002 496
Size: 1368 234 904 3778
Fragments arranged by size:
3778 1368 904 234
EcoO109I rG'GnC_Cy
Cuts at: 159 537 1107 1303 2103 2118 2607 3055 3073
Size: 378 570 196 800 15 489 448 18
Cuts at: 3073 3074 3184 3209 3423 159
Size: 1 110 25 214 3020
Fragments arranged by size:
3020 800 570 489 448 378 214 196
110 25 18 15 1
EcoRI G'AATT_C
Cuts at: 3288 3288
Size: 6284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 978 4303 978
Size: 3325 2959
FspI TGC'GCA
Cuts at: 4065 4065
Size: 6284
HaeII r_GCGC'y
Cuts at: 532 1208 2034 2572 3718 3966 4988 5967 532
Size: 676 826 538 1146 248 1022 979 849
Fragments arranged by size:
1146 1022 979 849 826 676 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 2394 2426 4126 4156 4158 4188 736
Size: 32 1626 32 1700 30 2 30 2832
Fragments arranged by size:
2832 1700 1626 32 32 30 30 2
HincII GTy'rAC
Cuts at: 900 1230 3323 900
Size: 330 2093 3861
Fragments arranged by size:
3861 2093 330
KasI G'GCGC_C
Cuts at: 3714 3962 3714
Size: 248 6036
KpnI G_GTAC'C
Cuts at: 3304 3304
Size: 6284
MluI A'CGCG_T
Cuts at: 5717 5717
Size: 6284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4955 5139 4955
Size: 184 6100
MscI TGG'CCA
Cuts at: 230 289 712 1324 4045 230
Size: 59 423 612 2721 2469
Fragments arranged by size:
2721 2469 612 423 59
MslI CAynn'nnrTG
Cuts at: 575 4273 4400 575
Size: 3698 127 2459
Fragments arranged by size:
3698 2459 127
MspA1I CmG'CkG
Cuts at: 133 514 1599 1641 1701 1719 1905 2277 2751
Size: 381 1085 42 60 18 186 372 474
Cuts at: 2751 3199 3395 3709 4069 5082 5327 133
Size: 448 196 314 360 1013 245 1090
Fragments arranged by size:
1090 1085 1013 474 448 381 372 360
314 245 196 186 60 42 18
NaeI GCC'GGC
Cuts at: 4465 4465
Size: 6284
NarI GG'CG_CC
Cuts at: 3715 3963 3715
Size: 248 6036
NcoI C'CATG_G
Cuts at: 231 713 2988 231
Size: 482 2275 3527
Fragments arranged by size:
3527 2275 482
NgoMIV G'CCGG_C
Cuts at: 4463 4463
Size: 6284
NspI r_CATG'y
Cuts at: 1143 1197 2323 3012 3337 3837 4744 1143
Size: 54 1126 689 325 500 907 2683
Fragments arranged by size:
2683 1126 907 689 500 325 54
PasI CC'CwG_GG
Cuts at: 2913 2913
Size: 6284
PciI A'CATG_T
Cuts at: 4740 4740
Size: 6284
PfoI T'CCnGG_A
Cuts at: 218 971 1562 1919 2387 218
Size: 753 591 357 468 4115
Fragments arranged by size:
4115 753 591 468 357
PmeI GTTT'AAAC
Cuts at: 3283 3283
Size: 6284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 343 375 1798 1830 2584 2616 5423 5455 343
Size: 32 1423 32 754 32 2807 32 1172
Fragments arranged by size:
2807 1423 1172 754 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 159 2103 2118 159
Size: 1944 15 4325
Fragments arranged by size:
4325 1944 15
PshAI GACnn'nnGTC
Cuts at: 873 905 873
Size: 32 6252
PspOMI G'GGCC_C
Cuts at: 3073 3183 3208 3073
Size: 110 25 6149
Fragments arranged by size:
6149 110 25
PstI C_TGCA'G
Cuts at: 1049 1455 1700 2051 2252 2372 2780 3331 4016
Size: 406 245 351 201 120 408 551 685
Cuts at: 4016 1049
Size: 3317
Fragments arranged by size:
3317 685 551 408 406 351 245 201
120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6284
PvuII CAG'CTG
Cuts at: 133 514 1599 1641 1905 2277 3709 4069 133
Size: 381 1085 42 264 372 1432 360 2348
Fragments arranged by size:
2348 1432 1085 381 372 360 264 42
RsrII CG'GwC_CG
Cuts at: 4479 4479
Size: 6284
SacI G_AGCT'C
Cuts at: 179 1919 2240 3298 179
Size: 1740 321 1058 3165
Fragments arranged by size:
3165 1740 1058 321
SacII CC_GC'GG
Cuts at: 3200 3200
Size: 6284
SalI G'TCGA_C
Cuts at: 898 3321 898
Size: 2423 3861
SapI GCTCTTCn'nnn_
Cuts at: 184 211 2489 4307 4517 184
Size: 27 2278 1818 210 1951
Fragments arranged by size:
2278 1951 1818 210 27
SbfI CC_TGCA'GG
Cuts at: 3331 3331
Size: 6284
ScaI AGT'ACT
Cuts at: 883 2038 5801 883
Size: 1155 3763 1366
Fragments arranged by size:
3763 1366 1155
SfcI C'TryA_G
Cuts at: 33 306 1045 1451 1696 2047 2248 2368 2776
Size: 273 739 406 245 351 201 120 408
Cuts at: 2776 3327 4012 5005 5196 33
Size: 551 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 551 408 406 351
273 245 201 191 120
SfoI GGC'GCC
Cuts at: 3716 3964 3716
Size: 248 6036
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6284
SmaI CCC'GGG
Cuts at: 262 1315 3207 3306 262
Size: 1053 1892 99 3240
Fragments arranged by size:
3240 1892 1053 99
SmlI C'TyrA_G
Cuts at: 923 1168 1708 2479 2488 2812 3439 4354 4846
Size: 245 540 771 9 324 627 915 492
Cuts at: 4846 5108 923
Size: 262 2099
Fragments arranged by size:
2099 915 771 627 540 492 324 262
245 9
SnaBI TAC'GTA
Cuts at: 3276 3276
Size: 6284
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6284
SphI G_CATG'C
Cuts at: 2323 3012 3337 3837 2323
Size: 689 325 500 4770
Fragments arranged by size:
4770 689 500 325
SrfI GCCC'GGGC
Cuts at: 3207 3207
Size: 6284
StuI AGG'CCT
Cuts at: 2839 2839
Size: 6284
StyI C'CwwG_G
Cuts at: 231 713 755 1016 1830 2988 3418 3772 231
Size: 482 42 261 814 1158 430 354 2743
Fragments arranged by size:
2743 1158 814 482 430 354 261 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1101 3867 4000 4459 4668 999
Size: 102 2766 133 459 209 2615
Fragments arranged by size:
2766 2615 459 209 133 102
TatI w'GTAC_w
Cuts at: 881 2036 2060 2282 2417 5799 881
Size: 1155 24 222 135 3382 1366
Fragments arranged by size:
3382 1366 1155 222 135 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 3084 3116 3253 3285 5801 5833 2137
Size: 32 915 32 137 32 2516 32 2588
Fragments arranged by size:
2588 2516 915 137 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4081 4081
Size: 6284
XbaI T'CTAG_A
Cuts at: 471 3315 471
Size: 2844 3440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 238 669 993 2190 2880 3247 3779 238
Size: 431 324 1197 690 367 532 2743
Fragments arranged by size:
2743 1197 690 532 431 367 324
XhoI C'TCGA_G
Cuts at: 1168 1168
Size: 6284
XmaI C'CCGG_G
Cuts at: 260 1313 3205 3304 260
Size: 1053 1892 99 3240
Fragments arranged by size:
3240 1892 1053 99
XmnI GAAnn'nnTTC
Cuts at: 2383 2383
Size: 6284
ZraI GAC'GTC
Cuts at: 747 747
Size: 6284
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AleI AscI AvrII BaeI BfrBI BmtI BsiWI
BsrGI BssHII BstEII BstXI ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SspI SwaI