Restriction map for
pF1KSDA0887
(Circular) (Six-base) MAPSORT of: pF1KSDA0887.seq Check: 3337 from: 1 to: 4474
pF1KSDA0887 4474 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4474
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4474
AclI AA'CG_TT
Cuts at: 4314 4314
Size: 4474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 859 1358 2297 2729 3478 3746 734
Size: 125 499 939 432 749 268 1462
Fragments arranged by size:
1462 939 749 499 432 268 125
AflIII A'CryG_T
Cuts at: 1824 2930 3907 1824
Size: 1106 977 2391
Fragments arranged by size:
2391 1106 977
AgeI A'CCGG_T
Cuts at: 2862 2862
Size: 4474
AlwNI CAG_nnn'CTG
Cuts at: 1777 3346 1777
Size: 1569 2905
ApaLI G'TGCA_C
Cuts at: 707 3244 707
Size: 2537 1937
ApoI r'AATT_y
Cuts at: 1137 1476 3827 4083 1137
Size: 339 2351 256 1528
Fragments arranged by size:
2351 1528 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4474
AvaI C'yCGr_G
Cuts at: 1241 1492 1241
Size: 251 4223
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4474
BanI G'GyrC_C
Cuts at: 132 1488 1902 2150 2185 132
Size: 1356 414 248 35 2421
Fragments arranged by size:
2421 1356 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3444
BbeI G_GCGC'C
Cuts at: 136 1906 2154 136
Size: 1770 248 2456
Fragments arranged by size:
2456 1770 248
BbsI GAAGACnn'nnnn_
Cuts at: 576 576
Size: 4474
BciVI GTATCCnnnnn_n'
Cuts at: 772 2370 3133 4474 772
Size: 1598 763 1341 772
Fragments arranged by size:
1598 1341 772 763
BclI T'GATC_A
Cuts at: 666 1992 666
Size: 1326 3148
BglI GCCn_nnn'nGGC
Cuts at: 1018 1018
Size: 4474
BglII A'GATC_T
Cuts at: 1987 4026 1987
Size: 2039 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4474
Bme1580I G_kGCm'C
Cuts at: 576 711 2097 2190 3248 576
Size: 135 1386 93 1058 1802
Fragments arranged by size:
1802 1386 1058 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4246 1684
Size: 81 335 2146 1912
Fragments arranged by size:
2146 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 1648 2527 3021 3319 360
Size: 1288 879 494 298 1515
Fragments arranged by size:
1515 1288 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 792 4178 792
Size: 3386 1088
BsaAI yAC'GTr
Cuts at: 1464 1825 1464
Size: 361 4113
BsaBI GATnn'nnATC
Cuts at: 6 1986 4025 6
Size: 1980 2039 455
Fragments arranged by size:
2039 1980 455
BsaHI Gr'CG_yC
Cuts at: 133 1903 2151 2855 4351 133
Size: 1770 248 704 1496 256
Fragments arranged by size:
1770 1496 704 256 248
BsaWI w'CCGG_w
Cuts at: 434 1661 2182 2862 3136 3283 3694 434
Size: 1227 521 680 274 147 411 1214
Fragments arranged by size:
1227 1214 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 1078 1105 1108 1135 1393 154
Size: 924 27 3 27 258 3235
Fragments arranged by size:
3235 924 258 27 27 3
BseYI C'CCAG_C
Cuts at: 1897 2655 3234 3730 1897
Size: 758 579 496 2641
Fragments arranged by size:
2641 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3270 104
Size: 1910 46 1210 1308
Fragments arranged by size:
1910 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 711 1486 2264 2454 3248 711
Size: 775 778 190 794 1937
Fragments arranged by size:
1937 794 778 775 190
BsmI GAATG_Cn'
Cuts at: 1174 1174
Size: 4474
BsmBI CGTCTCn'nnnn_
Cuts at: 1119 1245 1119
Size: 126 4348
Bsp1286I G_dGCh'C
Cuts at: 576 711 1486 2097 2190 2264 2454 2516 3248
Size: 135 775 611 93 74 190 62 732
Cuts at: 3248 576
Size: 1802
Fragments arranged by size:
1802 775 732 611 190 135 93 74
62
BspEI T'CCGG_A
Cuts at: 3694 3694
Size: 4474
BspHI T'CATG_A
Cuts at: 3650 3650
Size: 4474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 324 1522 1736 2038 2419 324
Size: 1198 214 302 381 2379
Fragments arranged by size:
2379 1198 381 302 214
BsrBI CCG'CTC
Cuts at: 50 144 1068 2764 2818 4304 4469 50
Size: 94 924 1696 54 1486 165 55
Fragments arranged by size:
1696 1486 924 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4474
BsrFI r'CCGG_y
Cuts at: 2470 2651 2862 2470
Size: 181 211 4082
Fragments arranged by size:
4082 211 181
BsrGI T'GTAC_A
Cuts at: 264 264
Size: 4474
BssSI C'ACGA_G
Cuts at: 2743 3103 2743
Size: 360 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4474
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4474
BstYI r'GATC_y
Cuts at: 1 147 189 356 1497 1964 1987 2322 2568
Size: 146 42 167 1141 467 23 335 246
Cuts at: 2568 3582 3668 3680 4026 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1141 1014 467 449 346 335 246 167
146 86 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 137 773 137
Size: 636 3838
BtgI C'CryG_G
Cuts at: 125 125
Size: 4474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4130
Fragments arranged by size:
4130 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4474
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4474
DraIII CAC_nnn'GTG
Cuts at: 706 706
Size: 4474
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3038 2178
Size: 860 3614
EaeI y'GGCC_r
Cuts at: 824 2057 2231 2622 2649 3996 824
Size: 1233 174 391 27 1347 1302
Fragments arranged by size:
1347 1302 1233 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4474
EarI CTCTTCn'nnn_
Cuts at: 482 551 751 2495 2705 482
Size: 69 200 1744 210 2251
Fragments arranged by size:
2251 1744 210 200 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1098 2680 2992 3138 4284 1098
Size: 1582 312 146 1146 1288
Fragments arranged by size:
1582 1288 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 859 1358 2297 2729 3478 3746 734
Size: 125 499 939 432 749 268 1462
Fragments arranged by size:
1462 939 749 499 432 268 125
EcoO109I rG'GnC_Cy
Cuts at: 1446 1611 1446
Size: 165 4309
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1236 2491 1236
Size: 1255 3219
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4474
HaeII r_GCGC'y
Cuts at: 136 1906 2154 3178 4157 136
Size: 1770 248 1024 979 453
Fragments arranged by size:
1770 1024 979 453 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 770 802 2314 2344 2346 2376 770
Size: 32 1512 30 2 30 2868
Fragments arranged by size:
2868 1512 32 30 30 2
HincII GTy'rAC
Cuts at: 1511 1511
Size: 4474
KasI G'GCGC_C
Cuts at: 132 1902 2150 132
Size: 1770 248 2456
Fragments arranged by size:
2456 1770 248
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4474
MluI A'CGCG_T
Cuts at: 3907 3907
Size: 4474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 3145 3329 1145
Size: 2000 184 2290
Fragments arranged by size:
2290 2000 184
MscI TGG'CCA
Cuts at: 826 2233 826
Size: 1407 3067
MslI CAynn'nnrTG
Cuts at: 662 739 832 2461 2588 662
Size: 77 93 1629 127 2548
Fragments arranged by size:
2548 1629 127 93 77
MspA1I CmG'CkG
Cuts at: 346 1583 1897 2257 3272 3517 346
Size: 1237 314 360 1015 245 1303
Fragments arranged by size:
1303 1237 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4474
NarI GG'CG_CC
Cuts at: 133 1903 2151 133
Size: 1770 248 2456
Fragments arranged by size:
2456 1770 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4474
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4474
NruI TCG'CGA
Cuts at: 2835 2835
Size: 4474
NspI r_CATG'y
Cuts at: 758 1525 2025 2934 758
Size: 767 500 909 2298
Fragments arranged by size:
2298 909 767 500
PciI A'CATG_T
Cuts at: 2930 2930
Size: 4474
PflMI CCAn_nnn'nTGG
Cuts at: 231 231
Size: 4474
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3613 3645 3613
Size: 32 4442
PpuMI rG'GwC_Cy
Cuts at: 1446 1446
Size: 4474
PstI C_TGCA'G
Cuts at: 182 335 1519 2204 182
Size: 153 1184 685 2452
Fragments arranged by size:
2452 1184 685 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4474
PvuII CAG'CTG
Cuts at: 346 1897 2257 346
Size: 1551 360 2563
Fragments arranged by size:
2563 1551 360
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4474
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4474
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4474
SapI GCTCTTCn'nnn_
Cuts at: 482 751 2495 2705 482
Size: 269 1744 210 2251
Fragments arranged by size:
2251 1744 269 210
SbfI CC_TGCA'GG
Cuts at: 335 1519 335
Size: 1184 3290
ScaI AGT'ACT
Cuts at: 3991 3991
Size: 4474
SexAI A'CCwGG_T
Cuts at: 941 941
Size: 4474
SfcI C'TryA_G
Cuts at: 33 178 331 360 1402 1515 2200 3195 3386
Size: 145 153 29 1042 113 685 995 191
Cuts at: 3386 33
Size: 1121
Fragments arranged by size:
1121 1042 995 685 191 153 145 113
29
SfiI GGCCn_nnn'nGGCC
Cuts at: 1018 1018
Size: 4474
SfoI GGC'GCC
Cuts at: 134 1904 2152 134
Size: 1770 248 2456
Fragments arranged by size:
2456 1770 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4474
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4474
SmlI C'TyrA_G
Cuts at: 375 1241 1627 2542 3036 3298 375
Size: 866 386 915 494 262 1551
Fragments arranged by size:
1551 915 866 494 386 262
SnaBI TAC'GTA
Cuts at: 1464 1464
Size: 4474
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4474
SphI G_CATG'C
Cuts at: 758 1525 2025 758
Size: 767 500 3207
Fragments arranged by size:
3207 767 500
StuI AGG'CCT
Cuts at: 878 1012 878
Size: 134 4340
StyI C'CwwG_G
Cuts at: 125 230 388 1606 1960 125
Size: 105 158 1218 354 2639
Fragments arranged by size:
2639 1218 354 158 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2055 2188 2647 2858 2055
Size: 133 459 211 3671
Fragments arranged by size:
3671 459 211 133
TatI w'GTAC_w
Cuts at: 264 3989 264
Size: 3725 749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3991 4023 3991
Size: 32 4442
Tth111I GACn'n_nGTC
Cuts at: 2269 2269
Size: 4474
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1967 1967
Size: 4474
XhoI C'TCGA_G
Cuts at: 1241 1241
Size: 4474
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4474
XmnI GAAnn'nnTTC
Cuts at: 1325 1325
Size: 4474
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SgrAI SrfI SspI SwaI ZraI