Restriction map for
pF1KSDA0891
(Circular) (Six-base) MAPSORT of: pF1KSDA0891.seq Check: 9491 from: 1 to: 7093
pF1KSDA0891 7093 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 586 1155 1648 586
Size: 569 493 6031
Fragments arranged by size:
6031 569 493
AccI GT'mk_AC
Cuts at: 3713 4129 3713
Size: 416 6677
Acc65I G'GTAC_C
Cuts at: 3047 4107 3047
Size: 1060 6033
AclI AA'CG_TT
Cuts at: 6933 6933
Size: 7093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2560 4916 5348 6097 6365 2560
Size: 2356 432 749 268 3288
Fragments arranged by size:
3288 2356 749 432 268
AfeI AGC'GCT
Cuts at: 1261 2545 3208 3451 1261
Size: 1284 663 243 4903
Fragments arranged by size:
4903 1284 663 243
AflIII A'CryG_T
Cuts at: 1025 3733 4043 4443 5549 6526 1025
Size: 2708 310 400 1106 977 1592
Fragments arranged by size:
2708 1592 1106 977 400 310
AgeI A'CCGG_T
Cuts at: 5481 5481
Size: 7093
AhdI GACnn_n'nnGTC
Cuts at: 2376 2376
Size: 7093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 2815 694
Size: 2121 4972
AlwNI CAG_nnn'CTG
Cuts at: 931 1668 2923 3268 3876 4396 5965 931
Size: 737 1255 345 608 520 1569 2059
Fragments arranged by size:
2059 1569 1255 737 608 520 345
ApaI G_GGCC'C
Cuts at: 167 1006 1145 2984 3917 3935 3952 167
Size: 839 139 1839 933 18 17 3308
Fragments arranged by size:
3308 1839 933 839 139 18 17
ApaLI G'TGCA_C
Cuts at: 1021 1200 3632 5863 1021
Size: 179 2432 2231 2251
Fragments arranged by size:
2432 2251 2231 179
ApoI r'AATT_y
Cuts at: 801 2954 4095 6446 6702 801
Size: 2153 1141 2351 256 1192
Fragments arranged by size:
2351 2153 1192 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7093
AvaI C'yCGr_G
Cuts at: 838 952 1131 1391 1679 2192 2906 3284 3349
Size: 114 179 260 288 513 714 378 65
Cuts at: 3349 4111 838
Size: 762 3820
Fragments arranged by size:
3820 762 714 513 378 288 260 179
114 65
AvrII C'CTAG_G
Cuts at: 241 3822 241
Size: 3581 3512
BamHI G'GATC_C
Cuts at: 3258 4116 3258
Size: 858 6235
BanI G'GyrC_C
Cuts at: 702 1527 1804 2145 2472 3047 3345 3416 3897
Size: 825 277 341 327 575 298 71 481
Cuts at: 3897 4009 4107 4521 4769 4804 702
Size: 112 98 414 248 35 2991
Fragments arranged by size:
2991 825 575 481 414 341 327 298
277 248 112 98 71 35
BanII G_rGCy'C
Cuts at: 167 793 953 1006 1145 2201 2785 2864 2984
Size: 626 160 53 139 1056 584 79 120
Cuts at: 2984 3917 3935 3952 4105 5135 167
Size: 933 18 17 153 1030 2125
Fragments arranged by size:
2125 1056 1030 933 626 584 160 153
139 120 79 53 18 17
BbeI G_GCGC'C
Cuts at: 3420 4525 4773 3420
Size: 1105 248 5740
Fragments arranged by size:
5740 1105 248
BbsI GAAGACnn'nnnn_
Cuts at: 440 612 440
Size: 172 6921
BbvCI CC'TCA_GC
Cuts at: 315 2006 2885 3254 3836 3884 315
Size: 1691 879 369 582 48 3524
Fragments arranged by size:
3524 1691 879 582 369 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 190 224 3562 3596 190
Size: 34 3338 34 3687
Fragments arranged by size:
3687 3338 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4071 4989 5752 7093 4071
Size: 918 763 1341 4071
Fragments arranged by size:
4071 1341 918 763
BclI T'GATC_A
Cuts at: 4611 4611
Size: 7093
BfrBI ATG'CAT
Cuts at: 1523 1523
Size: 7093
BglI GCCn_nnn'nGGC
Cuts at: 788 814 1846 3277 3853 788
Size: 26 1032 1431 576 4028
Fragments arranged by size:
4028 1431 1032 576 26
BglII A'GATC_T
Cuts at: 1040 4606 6645 1040
Size: 3566 2039 1488
Fragments arranged by size:
3566 2039 1488
BlpI GC'TnA_GC
Cuts at: 4203 4203
Size: 7093
Bme1580I G_kGCm'C
Cuts at: 167 1006 1025 1145 1204 1807 2095 2150 2477
Size: 839 19 120 59 603 288 55 327
Cuts at: 2477 2942 2984 3636 3917 3935 3952 4012 4716
Size: 465 42 652 281 18 17 60 704
Cuts at: 4716 4809 5867 167
Size: 93 1058 1393
Fragments arranged by size:
1393 1058 839 704 652 603 465 327
288 281 120 93 60 59 55 42
19 18 17
BmgBI CAC'GTC
Cuts at: 3672 3672
Size: 7093
BmrI ACTGGGnnnn_n'
Cuts at: 844 1543 3081 3951 4303 4384 4719 6865 844
Size: 699 1538 870 352 81 335 2146 1072
Fragments arranged by size:
2146 1538 1072 870 699 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3012 5458 3012
Size: 2446 4647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 449 565 842 1294 2839 2848 3178 3898
Size: 253 116 277 452 1545 9 330 720
Cuts at: 3898 4095 196
Size: 197 3194
Fragments arranged by size:
3194 1545 720 452 330 277 253 197
116 9
Bpu10I CC'TnA_GC
Cuts at: 315 1174 2006 2429 2858 2885 3254 3526 3836
Size: 859 832 423 429 27 369 272 310
Cuts at: 3836 3884 315
Size: 48 3524
Fragments arranged by size:
3524 859 832 429 423 369 310 272
48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1041 3430 4267 5146 5640 5938 386
Size: 655 2389 837 879 494 298 1541
Fragments arranged by size:
2389 1541 879 837 655 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 263 1960 2115 2834 6797 263
Size: 1697 155 719 3963 559
Fragments arranged by size:
3963 1697 719 559 155
BsaAI yAC'GTr
Cuts at: 716 1026 1032 3961 4083 4444 716
Size: 310 6 2929 122 361 3365
Fragments arranged by size:
3365 2929 361 310 122 6
BsaBI GATnn'nnATC
Cuts at: 6 236 4605 6644 6
Size: 230 4369 2039 455
Fragments arranged by size:
4369 2039 455 230
BsaHI Gr'CG_yC
Cuts at: 1758 3417 4522 4770 5474 6970 1758
Size: 1659 1105 248 704 1496 1881
Fragments arranged by size:
1881 1659 1496 1105 704 248
BsaWI w'CCGG_w
Cuts at: 869 3992 4280 4801 5481 5755 5902 6313 869
Size: 3123 288 521 680 274 147 411 1649
Fragments arranged by size:
3123 1649 680 521 411 288 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 447 477 507 1550 1580 2846 2876 3208 3238
Size: 30 30 1043 30 1266 30 332 30
Cuts at: 3238 3346 3376 447
Size: 108 30 4164
Fragments arranged by size:
4164 1266 1043 332 108 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 389 421 811 922 1399 1585 1588 2386 3367
Size: 32 390 111 477 186 3 798 981
Cuts at: 3367 3568 3901 3904 389
Size: 201 333 3 3578
Fragments arranged by size:
3578 981 798 477 390 333 201 186
111 32 3 3
BseYI C'CCAG_C
Cuts at: 331 1262 2476 2824 2941 3162 3565 4516 5274
Size: 931 1214 348 117 221 403 951 758
Cuts at: 5274 5853 6349 331
Size: 579 496 1075
Fragments arranged by size:
1214 1075 951 931 758 579 496 403
348 221 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 664 1111 1271 1909 2519 2775 3460 515
Size: 149 447 160 638 610 256 685 4148
Fragments arranged by size:
4148 685 638 610 447 256 160 149
BsiEI CG_ry'CG
Cuts at: 104 4633 4679 5889 104
Size: 4529 46 1210 1308
Fragments arranged by size:
4529 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1025 1204 1362 2864 3636 3812 4040 4105 4883
Size: 179 158 1502 772 176 228 65 778
Cuts at: 4883 5073 5867 1025
Size: 190 794 2251
Fragments arranged by size:
2251 1502 794 778 772 228 190 179
176 158 65
BsmI GAATG_Cn'
Cuts at: 1943 3078 1943
Size: 1135 5958
Bsp1286I G_dGCh'C
Cuts at: 167 793 953 1006 1025 1145 1204 1362 1807
Size: 626 160 53 19 120 59 158 445
Cuts at: 1807 2095 2150 2201 2477 2785 2864 2942 2984
Size: 288 55 51 276 308 79 78 42
Cuts at: 2984 3636 3812 3917 3935 3952 4012 4040 4105
Size: 652 176 105 18 17 60 28 65
Cuts at: 4105 4716 4809 4883 5073 5135 5867 167
Size: 611 93 74 190 62 732 1393
Fragments arranged by size:
1393 732 652 626 611 445 308 288
276 190 176 160 158 120 105 93
79 78 74 65 62 60 59 55
53 51 42 28 19 18 17
BspEI T'CCGG_A
Cuts at: 869 6313 869
Size: 5444 1649
BspHI T'CATG_A
Cuts at: 441 1646 6269 441
Size: 1205 4623 1265
Fragments arranged by size:
4623 1265 1205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 586 787 1155 1648 2315 2673 3787 4141 4355
Size: 201 368 493 667 358 1114 354 214
Cuts at: 4355 4657 5038 586
Size: 302 381 2641
Fragments arranged by size:
2641 1114 667 493 381 368 358 354
302 214 201
BsrBI CCG'CTC
Cuts at: 50 1709 2436 5383 5437 6923 7088 50
Size: 1659 727 2947 54 1486 165 55
Fragments arranged by size:
2947 1659 1486 727 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5003 5003
Size: 7093
BsrFI r'CCGG_y
Cuts at: 2142 3271 3289 3761 3894 5089 5270 5481 2142
Size: 1129 18 472 133 1195 181 211 3754
Fragments arranged by size:
3754 1195 1129 472 211 181 133 18
BssSI C'ACGA_G
Cuts at: 1289 1927 4033 5362 5722 1289
Size: 638 2106 1329 360 2660
Fragments arranged by size:
2660 2106 1329 638 360
BstAPI GCAn_nnn'nTGC
Cuts at: 928 931 1309 1640 3408 3536 4468 928
Size: 3 378 331 1768 128 932 3553
Fragments arranged by size:
3553 1768 932 378 331 128 3
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7093
BstEII G'GTnAC_C
Cuts at: 2871 2871
Size: 7093
BstXI CCAn_nnnn'nTGG
Cuts at: 331 3243 331
Size: 2912 4181
BstYI r'GATC_y
Cuts at: 1 237 1040 3258 4116 4583 4606 4941 5187
Size: 236 803 2218 858 467 23 335 246
Cuts at: 5187 6201 6287 6299 6645 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2218 1014 858 803 467 449 346 335
246 236 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 297 1120 3335 3902 297
Size: 823 2215 567 3488
Fragments arranged by size:
3488 2215 823 567
BtgI C'CryG_G
Cuts at: 863 1466 1970 4013 863
Size: 603 504 2043 3943
Fragments arranged by size:
3943 2043 603 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5041 5222 5385 5041
Size: 181 163 6749
Fragments arranged by size:
6749 181 163
BtsI GCAGTG_nn'
Cuts at: 613 688 836 923 1204 2249 2797 3623 3672
Size: 75 148 87 281 1045 548 826 49
Cuts at: 3672 4334 613
Size: 662 3372
Fragments arranged by size:
3372 1045 826 662 548 281 148 87
75 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 923 958 923
Size: 35 7058
DraI TTT'AAA
Cuts at: 4090 4090
Size: 7093
DrdI GACnn_nn'nnGTC
Cuts at: 4797 5657 4797
Size: 860 6233
EaeI y'GGCC_r
Cuts at: 1763 2972 3291 3615 4676 4850 5241 5268 6615
Size: 1209 319 324 1061 174 391 27 1347
Cuts at: 6615 1763
Size: 2241
Fragments arranged by size:
2241 1347 1209 1061 391 324 319 174
27
EagI C'GGCC_G
Cuts at: 4676 4676
Size: 7093
EarI CTCTTCn'nnn_
Cuts at: 494 1574 1580 3328 3757 5114 5324 494
Size: 1080 6 1748 429 1357 210 2263
Fragments arranged by size:
2263 1748 1357 1080 429 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 3210 3780 5299 5611 5757 6903 2332
Size: 878 570 1519 312 146 1146 2522
Fragments arranged by size:
2522 1519 1146 878 570 312 146
EcoICRI GAG'CTC
Cuts at: 2862 4103 2862
Size: 1241 5852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 449 565 842 1294 2560 2839 2848 3178
Size: 253 116 277 452 1266 279 9 330
Cuts at: 3178 3898 4095 4916 5348 6097 6365 196
Size: 720 197 821 432 749 268 924
Fragments arranged by size:
1266 924 821 749 720 452 432 330
279 277 268 253 197 116 9
EcoNI CCTnn'n_nnAGG
Cuts at: 733 815 2062 733
Size: 82 1247 5764
Fragments arranged by size:
5764 1247 82
EcoO109I rG'GnC_Cy
Cuts at: 163 164 532 1002 1142 1269 1278 1932 2616
Size: 1 368 470 140 127 9 654 684
Cuts at: 2616 2932 3785 3826 3914 3922 3931 3932 3949
Size: 316 853 41 88 8 9 1 17
Cuts at: 3949 4230 163
Size: 281 3026
Fragments arranged by size:
3026 853 684 654 470 368 316 281
140 127 88 41 17 9 9 8
1 1
EcoRI G'AATT_C
Cuts at: 4095 4095
Size: 7093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5110 5110
Size: 7093
FseI GG_CCGG'CC
Cuts at: 3293 3293
Size: 7093
FspI TGC'GCA
Cuts at: 4872 4872
Size: 7093
HaeII r_GCGC'y
Cuts at: 1263 1795 2466 2547 3210 3420 3453 3684 4525
Size: 532 671 81 663 210 33 231 841
Cuts at: 4525 4773 5797 6776 1263
Size: 248 1024 979 1580
Fragments arranged by size:
1580 1024 979 841 671 663 532 248
231 210 81 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 243 273 275 838 870 940 972 1239
Size: 2 30 2 563 32 70 32 267
Cuts at: 1239 1271 2260 2292 2362 2394 2398 2430 3004
Size: 32 989 32 70 32 4 32 574
Cuts at: 3004 3036 3250 3282 4933 4963 4965 4995 241
Size: 32 214 32 1651 30 2 30 2339
Fragments arranged by size:
2339 1651 989 574 563 267 214 70
70 32 32 32 32 32 32 32
32 30 30 30 4 2 2 2
HincII GTy'rAC
Cuts at: 4130 4130
Size: 7093
HindIII A'AGCT_T
Cuts at: 514 514
Size: 7093
KasI G'GCGC_C
Cuts at: 3416 4521 4769 3416
Size: 1105 248 5740
Fragments arranged by size:
5740 1105 248
KpnI G_GTAC'C
Cuts at: 3051 4111 3051
Size: 1060 6033
MluI A'CGCG_T
Cuts at: 3733 6526 3733
Size: 2793 4300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1697 2504 3140 5764 5948 1351
Size: 346 807 636 2624 184 2496
Fragments arranged by size:
2624 2496 807 636 346 184
MscI TGG'CCA
Cuts at: 1765 2974 3617 4852 1765
Size: 1209 643 1235 4006
Fragments arranged by size:
4006 1235 1209 643
MslI CAynn'nnrTG
Cuts at: 1465 3059 5080 5207 1465
Size: 1594 2021 127 3351
Fragments arranged by size:
3351 2021 1594 127
MspA1I CmG'CkG
Cuts at: 331 543 765 2022 2511 2868 3007 3129 3405
Size: 212 222 1257 489 357 139 122 276
Cuts at: 3405 3561 3835 4202 4516 4876 5891 6136 331
Size: 156 274 367 314 360 1015 245 1288
Fragments arranged by size:
1288 1257 1015 489 367 360 357 314
276 274 245 222 212 156 139 122
NaeI GCC'GGC
Cuts at: 3291 3763 5272 3291
Size: 472 1509 5112
Fragments arranged by size:
5112 1509 472
NarI GG'CG_CC
Cuts at: 3417 4522 4770 3417
Size: 1105 248 5740
Fragments arranged by size:
5740 1105 248
NcoI C'CATG_G
Cuts at: 863 1466 863
Size: 603 6490
NgoMIV G'CCGG_C
Cuts at: 3289 3761 5270 3289
Size: 472 1509 5112
Fragments arranged by size:
5112 1509 472
NruI TCG'CGA
Cuts at: 5454 5454
Size: 7093
NsiI A_TGCA'T
Cuts at: 1525 1525
Size: 7093
NspI r_CATG'y
Cuts at: 1477 1523 4144 4644 5553 1477
Size: 46 2621 500 909 3017
Fragments arranged by size:
3017 2621 909 500 46
PasI CC'CwG_GG
Cuts at: 883 3791 3918 3926 883
Size: 2908 127 8 4050
Fragments arranged by size:
4050 2908 127 8
PciI A'CATG_T
Cuts at: 5549 5549
Size: 7093
PflMI CCAn_nnn'nTGG
Cuts at: 1555 2367 1555
Size: 812 6281
PfoI T'CCnGG_A
Cuts at: 1688 3859 1688
Size: 2171 4922
PmeI GTTT'AAAC
Cuts at: 4090 4090
Size: 7093
PmlI CAC'GTG
Cuts at: 716 1026 3961 716
Size: 310 2935 3848
Fragments arranged by size:
3848 2935 310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6232 6264 6232
Size: 32 7061
PpuMI rG'GwC_Cy
Cuts at: 532 1932 2932 3922 532
Size: 1400 1000 990 3703
Fragments arranged by size:
3703 1400 1000 990
PshAI GACnn'nnGTC
Cuts at: 2930 2930
Size: 7093
PspOMI G'GGCC_C
Cuts at: 163 1002 1141 2980 3913 3931 3948 163
Size: 839 139 1839 933 18 17 3308
Fragments arranged by size:
3308 1839 933 839 139 18 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1942 1974 2626 2658 1942
Size: 32 652 32 6377
Fragments arranged by size:
6377 652 32 32
PstI C_TGCA'G
Cuts at: 542 1048 3834 4138 4823 542
Size: 506 2786 304 685 2812
Fragments arranged by size:
2812 2786 685 506 304
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7093
PvuII CAG'CTG
Cuts at: 331 543 2868 3007 3405 3561 3835 4516 4876
Size: 212 2325 139 398 156 274 681 360
Cuts at: 4876 331
Size: 2548
Fragments arranged by size:
2548 2325 681 398 360 274 212 156
139
RsrII CG'GwC_CG
Cuts at: 5286 5286
Size: 7093
SacI G_AGCT'C
Cuts at: 2864 4105 2864
Size: 1241 5852
SalI G'TCGA_C
Cuts at: 4128 4128
Size: 7093
SanDI GG'GwC_CC
Cuts at: 3922 3922
Size: 7093
SapI GCTCTTCn'nnn_
Cuts at: 5114 5324 5114
Size: 210 6883
SbfI CC_TGCA'GG
Cuts at: 4138 4138
Size: 7093
ScaI AGT'ACT
Cuts at: 1064 6610 1064
Size: 5546 1547
SfcI C'TryA_G
Cuts at: 33 538 1044 3174 3830 4134 4819 5814 6005
Size: 505 506 2130 656 304 685 995 191
Cuts at: 6005 33
Size: 1121
Fragments arranged by size:
2130 1121 995 685 656 506 505 304
191
SfoI GGC'GCC
Cuts at: 3418 4523 4771 3418
Size: 1105 248 5740
Fragments arranged by size:
5740 1105 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7093
SgrAI Cr'CCGG_yG
Cuts at: 3761 3761
Size: 7093
SmaI CCC'GGG
Cuts at: 840 1133 1393 2908 3286 4113 840
Size: 293 260 1515 378 827 3820
Fragments arranged by size:
3820 1515 827 378 293 260
SmlI C'TyrA_G
Cuts at: 401 1056 3445 4246 5161 5655 5917 401
Size: 655 2389 801 915 494 262 1577
Fragments arranged by size:
2389 1577 915 801 655 494 262
SnaBI TAC'GTA
Cuts at: 4083 4083
Size: 7093
SpeI A'CTAG_T
Cuts at: 67 187 67
Size: 120 6973
SphI G_CATG'C
Cuts at: 4144 4644 4144
Size: 500 6593
SrfI GCCC'GGGC
Cuts at: 1393 3286 1393
Size: 1893 5200
StuI AGG'CCT
Cuts at: 3358 3394 3358
Size: 36 7057
StyI C'CwwG_G
Cuts at: 154 241 863 902 938 1415 1466 2105 2768
Size: 87 622 39 36 477 51 639 663
Cuts at: 2768 3236 3822 4225 4579 154
Size: 468 586 403 354 2668
Fragments arranged by size:
2668 663 639 622 586 477 468 403
354 87 51 39 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1285 1479 1822 3154 3830 3933 4674 4807 5266
Size: 194 343 1332 676 103 741 133 459
Cuts at: 5266 5477 1285
Size: 211 2901
Fragments arranged by size:
2901 1332 741 676 459 343 211 194
133 103
TatI w'GTAC_w
Cuts at: 1062 2072 3747 6608 1062
Size: 1010 1675 2861 1547
Fragments arranged by size:
2861 1675 1547 1010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 1550 1582 2846 2878 2886 2918 6610
Size: 32 1041 32 1264 32 8 32 3692
Cuts at: 6610 6642 477
Size: 32 928
Fragments arranged by size:
3692 1264 1041 928 32 32 32 32
32 8
Tth111I GACn'n_nGTC
Cuts at: 255 850 4888 255
Size: 595 4038 2460
Fragments arranged by size:
4038 2460 595
XbaI T'CTAG_A
Cuts at: 4122 4122
Size: 7093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 909 1105 2220 2814 4586 909
Size: 196 1115 594 1772 3416
Fragments arranged by size:
3416 1772 1115 594 196
XmaI C'CCGG_G
Cuts at: 838 1131 1391 2906 3284 4111 838
Size: 293 260 1515 378 827 3820
Fragments arranged by size:
3820 1515 827 378 293 260
XmnI GAAnn'nnTTC
Cuts at: 2958 2958
Size: 7093
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AscI BaeI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI DraIII EcoRV FspAI
HpaI MfeI NdeI NheI NotI PacI PsiI PspXI
SacII SexAI SfiI SspI SwaI XhoI ZraI