Restriction map for
pF1KSDA0895
(Circular) (Six-base) MAPSORT of: pF1KSDA0895.seq Check: 6366 from: 1 to: 4546
pF1KSDA0895 4546 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1301 1582 1301
Size: 281 4265
Acc65I G'GTAC_C
Cuts at: 1560 1560
Size: 4546
AclI AA'CG_TT
Cuts at: 4386 4386
Size: 4546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2801 3550 3818 2369
Size: 432 749 268 3097
Fragments arranged by size:
3097 749 432 268
AflII C'TTAA_G
Cuts at: 832 832
Size: 4546
AflIII A'CryG_T
Cuts at: 1896 3002 3979 1896
Size: 1106 977 2463
Fragments arranged by size:
2463 1106 977
AgeI A'CCGG_T
Cuts at: 2934 2934
Size: 4546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 194 580 194
Size: 386 4160
AlwNI CAG_nnn'CTG
Cuts at: 1849 3418 1849
Size: 1569 2977
ApaLI G'TGCA_C
Cuts at: 3316 3316
Size: 4546
ApoI r'AATT_y
Cuts at: 676 1313 1548 3899 4155 676
Size: 637 235 2351 256 1067
Fragments arranged by size:
2351 1067 637 256 235
AseI AT'TA_AT
Cuts at: 20 20
Size: 4546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
AvaI C'yCGr_G
Cuts at: 252 872 1369 1564 252
Size: 620 497 195 3234
Fragments arranged by size:
3234 620 497 195
BamHI G'GATC_C
Cuts at: 1569 1569
Size: 4546
BanI G'GyrC_C
Cuts at: 438 596 1560 1974 2222 2257 438
Size: 158 964 414 248 35 2727
Fragments arranged by size:
2727 964 414 248 158 35
BanII G_rGCy'C
Cuts at: 1558 2588 1558
Size: 1030 3516
BbeI G_GCGC'C
Cuts at: 1978 2226 1978
Size: 248 4298
BbsI GAAGACnn'nnnn_
Cuts at: 341 341
Size: 4546
BbvCI CC'TCA_GC
Cuts at: 629 958 629
Size: 329 4217
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3205 4546 2442
Size: 763 1341 2442
Fragments arranged by size:
2442 1341 763
BclI T'GATC_A
Cuts at: 215 2064 215
Size: 1849 2697
BglII A'GATC_T
Cuts at: 1524 2059 4098 1524
Size: 535 2039 1972
Fragments arranged by size:
2039 1972 535
BlpI GC'TnA_GC
Cuts at: 803 1656 803
Size: 853 3693
Bme1580I G_kGCm'C
Cuts at: 2169 2262 3320 2169
Size: 93 1058 3395
Fragments arranged by size:
3395 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1756 1837 2172 4318 1756
Size: 81 335 2146 1984
Fragments arranged by size:
2146 1984 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1454 2911 1454
Size: 1457 3089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 493 967 988 151
Size: 342 474 21 3709
Fragments arranged by size:
3709 474 342 21
Bpu10I CC'TnA_GC
Cuts at: 167 629 958 167
Size: 462 329 3755
Fragments arranged by size:
3755 462 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1720 2599 3093 3391 1720
Size: 879 494 298 2875
Fragments arranged by size:
2875 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4250 4250
Size: 4546
BsaAI yAC'GTr
Cuts at: 934 1536 1897 934
Size: 602 361 3583
Fragments arranged by size:
3583 602 361
BsaBI GATnn'nnATC
Cuts at: 6 1394 2058 4097 6
Size: 1388 664 2039 455
Fragments arranged by size:
2039 1388 664 455
BsaHI Gr'CG_yC
Cuts at: 1975 2223 2927 4423 1975
Size: 248 704 1496 2098
Fragments arranged by size:
2098 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1733 2254 2934 3208 3355 3766 1733
Size: 521 680 274 147 411 2513
Fragments arranged by size:
2513 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4546
BseYI C'CCAG_C
Cuts at: 382 1969 2727 3306 3802 382
Size: 1587 758 579 496 1126
Fragments arranged by size:
1587 1126 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 914
Size: 4546
BsiEI CG_ry'CG
Cuts at: 104 2086 2132 3342 104
Size: 1982 46 1210 1308
Fragments arranged by size:
1982 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1558 2336 2526 3320 1558
Size: 778 190 794 2784
Fragments arranged by size:
2784 794 778 190
BsmI GAATG_Cn'
Cuts at: 959 1085 959
Size: 126 4420
Bsp1286I G_dGCh'C
Cuts at: 1558 2169 2262 2336 2526 2588 3320 1558
Size: 611 93 74 190 62 732 2784
Fragments arranged by size:
2784 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3766 3766
Size: 4546
BspHI T'CATG_A
Cuts at: 3722 3722
Size: 4546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 872 1190 1594 1808 2110 2491 872
Size: 318 404 214 302 381 2927
Fragments arranged by size:
2927 404 381 318 302 214
BsrBI CCG'CTC
Cuts at: 50 2836 2890 4376 4541 50
Size: 2786 54 1486 165 55
Fragments arranged by size:
2786 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2456 2456
Size: 4546
BsrFI r'CCGG_y
Cuts at: 2542 2723 2934 2542
Size: 181 211 4154
Fragments arranged by size:
4154 211 181
BssSI C'ACGA_G
Cuts at: 2815 3175 2815
Size: 360 4186
BstAPI GCAn_nnn'nTGC
Cuts at: 1921 1921
Size: 4546
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4546
BstYI r'GATC_y
Cuts at: 1 414 1524 1569 2036 2059 2394 2640 3654
Size: 413 1110 45 467 23 335 246 1014
Cuts at: 3654 3740 3752 4098 1
Size: 86 12 346 449
Fragments arranged by size:
1110 1014 467 449 413 346 335 246
86 45 23 12
BstZ17I GTA'TAC
Cuts at: 1302 1302
Size: 4546
BtgI C'CryG_G
Cuts at: 1012 1455 1012
Size: 443 4103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2494 2675 2838 2494
Size: 181 163 4202
Fragments arranged by size:
4202 181 163
BtsI GCAGTG_nn'
Cuts at: 459 926 1787 459
Size: 467 861 3218
Fragments arranged by size:
3218 861 467
DraI TTT'AAA
Cuts at: 1188 1543 1188
Size: 355 4191
DrdI GACnn_nn'nnGTC
Cuts at: 2250 3110 2250
Size: 860 3686
EaeI y'GGCC_r
Cuts at: 1458 2129 2303 2694 2721 4068 1458
Size: 671 174 391 27 1347 1936
Fragments arranged by size:
1936 1347 671 391 174 27
EagI C'GGCC_G
Cuts at: 2129 2129
Size: 4546
EarI CTCTTCn'nnn_
Cuts at: 617 2567 2777 617
Size: 1950 210 2386
Fragments arranged by size:
2386 1950 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2752 3064 3210 4356 2752
Size: 312 146 1146 2942
Fragments arranged by size:
2942 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1556 1556
Size: 4546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 493 967 988 2369 2801 3550 3818 151
Size: 342 474 21 1381 432 749 268 879
Fragments arranged by size:
1381 879 749 474 432 342 268 21
EcoO109I rG'GnC_Cy
Cuts at: 861 1212 1434 1683 861
Size: 351 222 249 3724
Fragments arranged by size:
3724 351 249 222
EcoRI G'AATT_C
Cuts at: 1313 1548 1313
Size: 235 4311
EcoRV GAT'ATC
Cuts at: 537 537
Size: 4546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4546
FspI TGC'GCA
Cuts at: 2325 2325
Size: 4546
HaeII r_GCGC'y
Cuts at: 1978 2226 3250 4229 1978
Size: 248 1024 979 2295
Fragments arranged by size:
2295 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2386 2416 2418 2448 2386
Size: 30 2 30 4484
Fragments arranged by size:
4484 30 30 2
HincII GTy'rAC
Cuts at: 1583 1583
Size: 4546
HindIII A'AGCT_T
Cuts at: 154 642 154
Size: 488 4058
KasI G'GCGC_C
Cuts at: 1974 2222 1974
Size: 248 4298
KpnI G_GTAC'C
Cuts at: 1564 1564
Size: 4546
MluI A'CGCG_T
Cuts at: 3979 3979
Size: 4546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 928 1003 3217 3401 928
Size: 75 2214 184 2073
Fragments arranged by size:
2214 2073 184 75
MscI TGG'CCA
Cuts at: 2305 2305
Size: 4546
MslI CAynn'nnrTG
Cuts at: 142 1492 2533 2660 142
Size: 1350 1041 127 2028
Fragments arranged by size:
2028 1350 1041 127
MspA1I CmG'CkG
Cuts at: 633 1655 1969 2329 3344 3589 633
Size: 1022 314 360 1015 245 1590
Fragments arranged by size:
1590 1022 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 4546
NarI GG'CG_CC
Cuts at: 1975 2223 1975
Size: 248 4298
NcoI C'CATG_G
Cuts at: 1012 1455 1012
Size: 443 4103
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 4546
NruI TCG'CGA
Cuts at: 2907 2907
Size: 4546
NspI r_CATG'y
Cuts at: 1597 2097 3006 1597
Size: 500 909 3137
Fragments arranged by size:
3137 909 500
PciI A'CATG_T
Cuts at: 3002 3002
Size: 4546
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4546
PmlI CAC'GTG
Cuts at: 934 934
Size: 4546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 3717 3685
Size: 32 4514
PpuMI rG'GwC_Cy
Cuts at: 861 1212 1434 861
Size: 351 222 3973
Fragments arranged by size:
3973 351 222
PstI C_TGCA'G
Cuts at: 1451 1591 2276 1451
Size: 140 685 3721
Fragments arranged by size:
3721 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4546
PvuII CAG'CTG
Cuts at: 633 1969 2329 633
Size: 1336 360 2850
Fragments arranged by size:
2850 1336 360
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4546
SacI G_AGCT'C
Cuts at: 1558 1558
Size: 4546
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4546
SanDI GG'GwC_CC
Cuts at: 1434 1434
Size: 4546
SapI GCTCTTCn'nnn_
Cuts at: 2567 2777 2567
Size: 210 4336
SbfI CC_TGCA'GG
Cuts at: 1451 1591 1451
Size: 140 4406
ScaI AGT'ACT
Cuts at: 563 4063 563
Size: 3500 1046
SexAI A'CCwGG_T
Cuts at: 1295 1295
Size: 4546
SfcI C'TryA_G
Cuts at: 33 307 1447 1587 2272 3267 3458 33
Size: 274 1140 140 685 995 191 1121
Fragments arranged by size:
1140 1121 995 685 274 191 140
SfoI GGC'GCC
Cuts at: 1976 2224 1976
Size: 248 4298
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
SmaI CCC'GGG
Cuts at: 1566 1566
Size: 4546
SmlI C'TyrA_G
Cuts at: 832 1699 2614 3108 3370 832
Size: 867 915 494 262 2008
Fragments arranged by size:
2008 915 867 494 262
SnaBI TAC'GTA
Cuts at: 1536 1536
Size: 4546
SpeI A'CTAG_T
Cuts at: 67 499 67
Size: 432 4114
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4046
StyI C'CwwG_G
Cuts at: 1012 1455 1678 2032 1012
Size: 443 223 354 3526
Fragments arranged by size:
3526 443 354 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 839 2127 2260 2719 2930 839
Size: 1288 133 459 211 2455
Fragments arranged by size:
2455 1288 459 211 133
TatI w'GTAC_w
Cuts at: 561 4061 561
Size: 3500 1046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4063 4095 4063
Size: 32 4514
Tth111I GACn'n_nGTC
Cuts at: 2341 2341
Size: 4546
XbaI T'CTAG_A
Cuts at: 637 1575 637
Size: 938 3608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1019 2039 1019
Size: 1020 3526
XmaI C'CCGG_G
Cuts at: 1564 1564
Size: 4546
XmnI GAAnn'nnTTC
Cuts at: 155 237 155
Size: 82 4464
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI