Restriction map for
pF1KSDA0899
(Circular) (Six-base) MAPSORT of: pF1KSDA0899.seq Check: 8020 from: 1 to: 5956
pF1KSDA0899 5956 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 716 1703 2463 2472 716
Size: 987 760 9 4200
Fragments arranged by size:
4200 987 760 9
AatII G_ACGT'C
Cuts at: 661 841 661
Size: 180 5776
AccI GT'mk_AC
Cuts at: 2083 2992 2083
Size: 909 5047
Acc65I G'GTAC_C
Cuts at: 1508 2970 1508
Size: 1462 4494
AclI AA'CG_TT
Cuts at: 2643 5796 2643
Size: 3153 2803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1420 1567 2398 3779 4211 4960 5228 1273
Size: 147 147 831 1381 432 749 268 2001
Fragments arranged by size:
2001 1381 831 749 432 268 147 147
AflII C'TTAA_G
Cuts at: 2305 2305
Size: 5956
AflIII A'CryG_T
Cuts at: 2183 2567 3306 4412 5389 2183
Size: 384 739 1106 977 2750
Fragments arranged by size:
2750 1106 977 739 384
AgeI A'CCGG_T
Cuts at: 4344 4344
Size: 5956
AleI CACnn'nnGTG
Cuts at: 728 728
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1874 2188 1874
Size: 314 5642
AlwNI CAG_nnn'CTG
Cuts at: 943 1134 3259 4828 943
Size: 191 2125 1569 2071
Fragments arranged by size:
2125 2071 1569 191
ApaI G_GGCC'C
Cuts at: 1987 2146 1987
Size: 159 5797
ApaLI G'TGCA_C
Cuts at: 703 1182 4726 703
Size: 479 3544 1933
Fragments arranged by size:
3544 1933 479
ApoI r'AATT_y
Cuts at: 253 2314 2325 2599 2784 2958 5309 5565 253
Size: 2061 11 274 185 174 2351 256 644
Fragments arranged by size:
2351 2061 644 274 256 185 174 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 5956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5956
AvaI C'yCGr_G
Cuts at: 695 1665 2116 2974 695
Size: 970 451 858 3677
Fragments arranged by size:
3677 970 858 451
BamHI G'GATC_C
Cuts at: 2979 2979
Size: 5956
BanI G'GyrC_C
Cuts at: 1508 2520 2554 2970 3384 3632 3667 1508
Size: 1012 34 416 414 248 35 3797
Fragments arranged by size:
3797 1012 416 414 248 35 34
BanII G_rGCy'C
Cuts at: 1987 2146 2968 3998 1987
Size: 159 822 1030 3945
Fragments arranged by size:
3945 1030 822 159
BbeI G_GCGC'C
Cuts at: 2524 3388 3636 2524
Size: 864 248 4844
Fragments arranged by size:
4844 864 248
BbsI GAAGACnn'nnnn_
Cuts at: 162 1261 2240 162
Size: 1099 979 3878
Fragments arranged by size:
3878 1099 979
BciVI GTATCCnnnnn_n'
Cuts at: 1446 3852 4615 5956 1446
Size: 2406 763 1341 1446
Fragments arranged by size:
2406 1446 1341 763
BclI T'GATC_A
Cuts at: 458 755 3474 458
Size: 297 2719 2940
Fragments arranged by size:
2940 2719 297
BglI GCCn_nnn'nGGC
Cuts at: 1414 1414
Size: 5956
BglII A'GATC_T
Cuts at: 852 3469 5508 852
Size: 2617 2039 1300
Fragments arranged by size:
2617 2039 1300
BlpI GC'TnA_GC
Cuts at: 823 1351 1879 3066 823
Size: 528 528 1187 3713
Fragments arranged by size:
3713 1187 528 528
Bme1580I G_kGCm'C
Cuts at: 707 1186 1987 2146 2557 3579 3672 4730 707
Size: 479 801 159 411 1022 93 1058 1933
Fragments arranged by size:
1933 1058 1022 801 479 411 159 93
BmgBI CAC'GTC
Cuts at: 1269 1545 1806 1842 2037 2184 1269
Size: 276 261 36 195 147 5041
Fragments arranged by size:
5041 276 261 195 147 36
BmrI ACTGGGnnnn_n'
Cuts at: 3166 3247 3582 5728 3166
Size: 81 335 2146 3394
Fragments arranged by size:
3394 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4321 4321
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 994 1192 1381 1679 1939 2026 841
Size: 153 198 189 298 260 87 4771
Fragments arranged by size:
4771 298 260 198 189 153 87
Bpu10I CC'TnA_GC
Cuts at: 1828 2051 1828
Size: 223 5733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1954 3130 4009 4503 4801 1954
Size: 1176 879 494 298 3109
Fragments arranged by size:
3109 1176 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 970 1141 1655 2119 5660 970
Size: 171 514 464 3541 1266
Fragments arranged by size:
3541 1266 514 464 171
BsaAI yAC'GTr
Cuts at: 1491 2946 3307 1491
Size: 1455 361 4140
Fragments arranged by size:
4140 1455 361
BsaBI GATnn'nnATC
Cuts at: 6 1528 2341 2774 3468 5507 6
Size: 1522 813 433 694 2039 455
Fragments arranged by size:
2039 1522 813 694 455 433
BsaHI Gr'CG_yC
Cuts at: 658 838 2521 3385 3633 4337 5833 658
Size: 180 1683 864 248 704 1496 781
Fragments arranged by size:
1683 1496 864 781 704 248 180
BsaWI w'CCGG_w
Cuts at: 3143 3664 4344 4618 4765 5176 3143
Size: 521 680 274 147 411 3923
Fragments arranged by size:
3923 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 1933 1963 2076 2106 1165
Size: 30 738 30 113 30 5015
Fragments arranged by size:
5015 738 113 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1936 2145 1288
Size: 648 209 5099
Fragments arranged by size:
5099 648 209
BseYI C'CCAG_C
Cuts at: 1987 2061 2109 3379 4137 4716 5212 1987
Size: 74 48 1270 758 579 496 2731
Fragments arranged by size:
2731 1270 758 579 496 74 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1567 1684 1740 2497 2596 2698 2735 505
Size: 1062 117 56 757 99 102 37 3726
Fragments arranged by size:
3726 1062 757 117 102 99 56 37
BsiEI CG_ry'CG
Cuts at: 104 134 1317 1913 2456 3496 3542 4752 104
Size: 30 1183 596 543 1040 46 1210 1308
Fragments arranged by size:
1308 1210 1183 1040 596 543 46 30
BsiHKAI G_wGCw'C
Cuts at: 707 1186 2968 3746 3936 4730 707
Size: 479 1782 778 190 794 1933
Fragments arranged by size:
1933 1782 794 778 479 190
BsmI GAATG_Cn'
Cuts at: 1047 1047
Size: 5956
BsmBI CGTCTCn'nnnn_
Cuts at: 1228 1228
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 707 1186 1987 2146 2557 2968 3579 3672 3746
Size: 479 801 159 411 411 611 93 74
Cuts at: 3746 3936 3998 4730 707
Size: 190 62 732 1933
Fragments arranged by size:
1933 801 732 611 479 411 411 190
159 93 74 62
BspEI T'CCGG_A
Cuts at: 5176 5176
Size: 5956
BspHI T'CATG_A
Cuts at: 333 5132 333
Size: 4799 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 716 1106 1163 1703 1880 2114 2438 2463
Size: 339 390 57 540 177 234 324 25
Cuts at: 2463 2472 2864 3004 3218 3520 3901 377
Size: 9 392 140 214 302 381 2432
Fragments arranged by size:
2432 540 392 390 381 339 324 302
234 214 177 140 57 25 9
BsrBI CCG'CTC
Cuts at: 50 1263 1944 4246 4300 5786 5951 50
Size: 1213 681 2302 54 1486 165 55
Fragments arranged by size:
2302 1486 1213 681 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3866 3866
Size: 5956
BsrFI r'CCGG_y
Cuts at: 129 2873 3952 4133 4344 129
Size: 2744 1079 181 211 1741
Fragments arranged by size:
2744 1741 1079 211 181
BsrGI T'GTAC_A
Cuts at: 650 650
Size: 5956
BssHII G'CGCG_C
Cuts at: 1736 2468 1736
Size: 732 5224
BssSI C'ACGA_G
Cuts at: 1600 2176 4225 4585 1600
Size: 576 2049 360 2971
Fragments arranged by size:
2971 2049 576 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2676 3331 2676
Size: 655 5301
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 507 2445 507
Size: 1938 4018
BstYI r'GATC_y
Cuts at: 1 575 852 1669 2979 3446 3469 3804 4050
Size: 574 277 817 1310 467 23 335 246
Cuts at: 4050 5064 5150 5162 5508 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1310 1014 817 574 467 449 346 335
277 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 581 581
Size: 5956
BtgI C'CryG_G
Cuts at: 677 1289 1886 2138 2444 2456 677
Size: 612 597 252 306 12 4177
Fragments arranged by size:
4177 612 597 306 252 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 513 3904 4085 4248 513
Size: 3391 181 163 2221
Fragments arranged by size:
3391 2221 181 163
BtsI GCAGTG_nn'
Cuts at: 522 928 953 3197 522
Size: 406 25 2244 3281
Fragments arranged by size:
3281 2244 406 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2808 2843 2808
Size: 35 5921
DraI TTT'AAA
Cuts at: 258 792 2953 258
Size: 534 2161 3261
Fragments arranged by size:
3261 2161 534
DrdI GACnn_nn'nnGTC
Cuts at: 2716 3660 4520 2716
Size: 944 860 4152
Fragments arranged by size:
4152 944 860
EaeI y'GGCC_r
Cuts at: 131 485 554 1190 1340 1559 1889 3539 3713
Size: 354 69 636 150 219 330 1650 174
Cuts at: 3713 4104 4131 5478 131
Size: 391 27 1347 609
Fragments arranged by size:
1650 1347 636 609 391 354 330 219
174 150 69 27
EagI C'GGCC_G
Cuts at: 131 3539 131
Size: 3408 2548
EarI CTCTTCn'nnn_
Cuts at: 319 779 1382 1493 1586 1780 3977 4187 319
Size: 460 603 111 93 194 2197 210 2088
Fragments arranged by size:
2197 2088 603 460 210 194 111 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 442 4162 4474 4620 5766 442
Size: 3720 312 146 1146 632
Fragments arranged by size:
3720 1146 632 312 146
EcoICRI GAG'CTC
Cuts at: 2966 2966
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 994 1192 1273 1381 1420 1567 1679 1939
Size: 153 198 81 108 39 147 112 260
Cuts at: 1939 2026 2398 3779 4211 4960 5228 841
Size: 87 372 1381 432 749 268 1569
Fragments arranged by size:
1569 1381 749 432 372 268 260 198
153 147 112 108 87 81 39
EcoO109I rG'GnC_Cy
Cuts at: 159 585 1983 1984 2047 2143 3093 159
Size: 426 1398 1 63 96 950 3022
Fragments arranged by size:
3022 1398 950 426 96 63 1
EcoRI G'AATT_C
Cuts at: 2958 2958
Size: 5956
EcoRV GAT'ATC
Cuts at: 183 183
Size: 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 330 3973 330
Size: 3643 2313
FspI TGC'GCA
Cuts at: 1813 2887 3735 1813
Size: 1074 848 4034
Fragments arranged by size:
4034 1074 848
HaeII r_GCGC'y
Cuts at: 2078 2524 3388 3636 4660 5639 2078
Size: 446 864 248 1024 979 2395
Fragments arranged by size:
2395 1024 979 864 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 676 708 1654 1686 1933 1965 2173 2185 2205
Size: 32 946 32 247 32 208 12 20
Cuts at: 2205 2217 2488 2520 2786 2818 3796 3826 3828
Size: 12 271 32 266 32 978 30 2
Cuts at: 3828 3858 676
Size: 30 2774
Fragments arranged by size:
2774 978 946 271 266 247 208 32
32 32 32 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 2490 2993 2490
Size: 503 5453
KasI G'GCGC_C
Cuts at: 2520 3384 3632 2520
Size: 864 248 4844
Fragments arranged by size:
4844 864 248
KpnI G_GTAC'C
Cuts at: 1512 2974 1512
Size: 1462 4494
MluI A'CGCG_T
Cuts at: 5389 5389
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2314 2531 2626 4627 4811 1055
Size: 1259 217 95 2001 184 2200
Fragments arranged by size:
2200 2001 1259 217 184 95
MscI TGG'CCA
Cuts at: 487 1192 1891 3715 487
Size: 705 699 1824 2728
Fragments arranged by size:
2728 1824 705 699
MslI CAynn'nnrTG
Cuts at: 421 505 728 1085 3943 4070 421
Size: 84 223 357 2858 127 2307
Fragments arranged by size:
2858 2307 357 223 127 84
MspA1I CmG'CkG
Cuts at: 1284 1369 1680 2140 2168 2289 2580 3065 3379
Size: 85 311 460 28 121 291 485 314
Cuts at: 3379 3739 4754 4999 1284
Size: 360 1015 245 2241
Fragments arranged by size:
2241 1015 485 460 360 314 311 291
245 121 85 28
NaeI GCC'GGC
Cuts at: 131 4135 131
Size: 4004 1952
NarI GG'CG_CC
Cuts at: 2521 3385 3633 2521
Size: 864 248 4844
Fragments arranged by size:
4844 864 248
NcoI C'CATG_G
Cuts at: 677 677
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 129 4133 129
Size: 4004 1952
NruI TCG'CGA
Cuts at: 4317 4317
Size: 5956
NspI r_CATG'y
Cuts at: 1182 3007 3507 4416 1182
Size: 1825 500 909 2722
Fragments arranged by size:
2722 1825 909 500
PciI A'CATG_T
Cuts at: 4412 4412
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 726 726
Size: 5956
PfoI T'CCnGG_A
Cuts at: 857 1778 1799 1937 857
Size: 921 21 138 4876
Fragments arranged by size:
4876 921 138 21
PmeI GTTT'AAAC
Cuts at: 2953 2953
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5095 5127 5095
Size: 32 5924
PpuMI rG'GwC_Cy
Cuts at: 585 2047 585
Size: 1462 4494
PshAI GACnn'nnGTC
Cuts at: 2307 2307
Size: 5956
PspOMI G'GGCC_C
Cuts at: 1983 2142 1983
Size: 159 5797
PstI C_TGCA'G
Cuts at: 744 923 948 1139 1853 2435 3001 3686 744
Size: 179 25 191 714 582 566 685 3014
Fragments arranged by size:
3014 714 685 582 566 191 179 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5956
PvuII CAG'CTG
Cuts at: 1369 2289 2580 3379 3739 1369
Size: 920 291 799 360 3586
Fragments arranged by size:
3586 920 799 360 291
RsrII CG'GwC_CG
Cuts at: 4149 4149
Size: 5956
SacI G_AGCT'C
Cuts at: 2968 2968
Size: 5956
SacII CC_GC'GG
Cuts at: 2141 2141
Size: 5956
SalI G'TCGA_C
Cuts at: 2991 2991
Size: 5956
SapI GCTCTTCn'nnn_
Cuts at: 319 1586 3977 4187 319
Size: 1267 2391 210 2088
Fragments arranged by size:
2391 2088 1267 210
SbfI CC_TGCA'GG
Cuts at: 3001 3001
Size: 5956
ScaI AGT'ACT
Cuts at: 5473 5473
Size: 5956
SexAI A'CCwGG_T
Cuts at: 1441 1607 1441
Size: 166 5790
SfcI C'TryA_G
Cuts at: 33 282 740 919 944 1135 1849 2431 2997
Size: 249 458 179 25 191 714 582 566
Cuts at: 2997 3682 4677 4868 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 714 685 582 566 458 249
191 191 179 25
SfoI GGC'GCC
Cuts at: 2522 3386 3634 2522
Size: 864 248 4844
Fragments arranged by size:
4844 864 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5956
SmaI CCC'GGG
Cuts at: 2976 2976
Size: 5956
SmlI C'TyrA_G
Cuts at: 1969 2305 3109 4024 4518 4780 1969
Size: 336 804 915 494 262 3145
Fragments arranged by size:
3145 915 804 494 336 262
SnaBI TAC'GTA
Cuts at: 2946 2946
Size: 5956
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5956
SphI G_CATG'C
Cuts at: 3007 3507 3007
Size: 500 5456
StuI AGG'CCT
Cuts at: 562 562
Size: 5956
StyI C'CwwG_G
Cuts at: 140 327 677 3088 3442 140
Size: 187 350 2411 354 2654
Fragments arranged by size:
2654 2411 354 350 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 2717 2836 3537 3670 4129 4340 718
Size: 1999 119 701 133 459 211 2334
Fragments arranged by size:
2334 1999 701 459 211 133 119
TatI w'GTAC_w
Cuts at: 650 5471 650
Size: 4821 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1165 1197 5473 5505 1165
Size: 32 4276 32 1616
Fragments arranged by size:
4276 1616 32 32
Tth111I GACn'n_nGTC
Cuts at: 3751 3751
Size: 5956
XbaI T'CTAG_A
Cuts at: 2985 2985
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3449 3449
Size: 5956
XmaI C'CCGG_G
Cuts at: 2974 2974
Size: 5956
XmnI GAAnn'nnTTC
Cuts at: 1774 2686 1774
Size: 912 5044
ZraI GAC'GTC
Cuts at: 659 839 659
Size: 180 5776
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsiWI BstEII BstZ17I ClaI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PmlI PsiI PspXI PsrI SanDI
SfiI SgrAI SrfI SspI SwaI XhoI