Restriction map for
pF1KSDA0907
(Circular) (Six-base) MAPSORT of: pF1KSDA0907.seq Check: 3189 from: 1 to: 4829
pF1KSDA0907 4829 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1817 1867 1817
Size: 50 4779
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 367 461 2654 3086 3833 4101 266
Size: 101 94 2193 432 747 268 994
Fragments arranged by size:
2193 994 747 432 268 101 94
AflIII A'CryG_T
Cuts at: 469 2181 3285 4262 469
Size: 1712 1104 977 1036
Fragments arranged by size:
1712 1104 1036 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AlwNI CAG_nnn'CTG
Cuts at: 733 979 1711 2134 3701 733
Size: 246 732 423 1567 1861
Fragments arranged by size:
1861 1567 732 423 246
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 1833 4182 4438 1833
Size: 2349 256 2224
Fragments arranged by size:
2349 2224 256
AseI AT'TA_AT
Cuts at: 20 323 452 1115 20
Size: 303 129 663 3734
Fragments arranged by size:
3734 663 303 129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 488 542 1849 488
Size: 54 1307 3468
Fragments arranged by size:
3468 1307 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1109 1142 1109
Size: 33 4796
BamHI G'GATC_C
Cuts at: 1854 1854
Size: 4829
BanI G'GyrC_C
Cuts at: 164 265 928 1845 2259 2507 2542 164
Size: 101 663 917 414 248 35 2451
Fragments arranged by size:
2451 917 663 414 248 101 35
BanII G_rGCy'C
Cuts at: 1843 2873 1843
Size: 1030 3799
BbeI G_GCGC'C
Cuts at: 168 2263 2511 168
Size: 2095 248 2486
Fragments arranged by size:
2486 2095 248
BbsI GAAGACnn'nnnn_
Cuts at: 930 930
Size: 4829
BbvCI CC'TCA_GC
Cuts at: 1235 1235
Size: 4829
BciVI GTATCCnnnnn_n'
Cuts at: 1552 2727 3488 4829 1552
Size: 1175 761 1341 1552
Fragments arranged by size:
1552 1341 1175 761
BclI T'GATC_A
Cuts at: 2349 2349
Size: 4829
BfrBI ATG'CAT
Cuts at: 810 810
Size: 4829
BglII A'GATC_T
Cuts at: 2344 4381 2344
Size: 2037 2792
BlpI GC'TnA_GC
Cuts at: 1941 1941
Size: 4829
Bme1580I G_kGCm'C
Cuts at: 268 2454 2547 3603 268
Size: 2186 93 1056 1494
Fragments arranged by size:
2186 1494 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2122 2457 4601 2041
Size: 81 335 2144 2269
Fragments arranged by size:
2269 2144 335 81
BmtI G_CTAG'C
Cuts at: 1382 1382
Size: 4829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 4829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 176 755 1322 1346 1358 1590 173
Size: 3 579 567 24 12 232 3412
Fragments arranged by size:
3412 579 567 232 24 12 3
Bpu10I CC'TnA_GC
Cuts at: 1235 1235
Size: 4829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 2005 2884 3376 3674 485
Size: 1520 879 492 298 1640
Fragments arranged by size:
1640 1520 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4533 4533
Size: 4829
BsaAI yAC'GTr
Cuts at: 1821 2182 1821
Size: 361 4468
BsaBI GATnn'nnATC
Cuts at: 6 2343 4380 6
Size: 2337 2037 455
Fragments arranged by size:
2337 2037 455
BsaHI Gr'CG_yC
Cuts at: 165 2260 2508 3210 4706 165
Size: 2095 248 702 1496 288
Fragments arranged by size:
2095 1496 702 288 248
BsaWI w'CCGG_w
Cuts at: 653 2018 2539 3217 3491 3638 4049 653
Size: 1365 521 678 274 147 411 1433
Fragments arranged by size:
1433 1365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 261 291 1755 1785 261
Size: 30 1464 30 3305
Fragments arranged by size:
3305 1464 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1163 1223 1537 1163
Size: 60 314 4455
Fragments arranged by size:
4455 314 60
BseYI C'CCAG_C
Cuts at: 1454 1460 1466 1472 1786 2254 3012 3589 4085
Size: 6 6 6 314 468 758 577 496
Cuts at: 4085 1454
Size: 2198
Fragments arranged by size:
2198 758 577 496 468 314 6 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1649 1387
Size: 262 4567
BsiEI CG_ry'CG
Cuts at: 104 2371 2417 3625 104
Size: 2267 46 1208 1308
Fragments arranged by size:
2267 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1843 2621 2811 3603 1843
Size: 778 190 792 3069
Fragments arranged by size:
3069 792 778 190
BsmI GAATG_Cn'
Cuts at: 372 372
Size: 4829
BsmBI CGTCTCn'nnnn_
Cuts at: 1503 1503
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 268 1843 2454 2547 2621 2811 2873 3603 268
Size: 1575 611 93 74 190 62 730 1494
Fragments arranged by size:
1575 1494 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4049 4049
Size: 4829
BspHI T'CATG_A
Cuts at: 554 4005 554
Size: 3451 1378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1879 2093 2395 2776 1879
Size: 214 302 381 3932
Fragments arranged by size:
3932 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3121 3175 4659 4824 50
Size: 3071 54 1484 165 55
Fragments arranged by size:
3071 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2741 2741
Size: 4829
BsrFI r'CCGG_y
Cuts at: 1311 2827 3008 3217 1311
Size: 1516 181 209 2923
Fragments arranged by size:
2923 1516 209 181
BssSI C'ACGA_G
Cuts at: 3100 3458 3100
Size: 358 4471
BstAPI GCAn_nnn'nTGC
Cuts at: 1686 2206 1686
Size: 520 4309
BstBI TT'CG_AA
Cuts at: 110 3190 110
Size: 3080 1749
BstEII G'GTnAC_C
Cuts at: 237 1213 237
Size: 976 3853
BstXI CCAn_nnnn'nTGG
Cuts at: 336 681 336
Size: 345 4484
BstYI r'GATC_y
Cuts at: 1 1854 2321 2344 2679 2925 3937 4023 4035
Size: 1853 467 23 335 246 1012 86 12
Cuts at: 4035 4381 1
Size: 346 449
Fragments arranged by size:
1853 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1653 1653
Size: 4829
BtgI C'CryG_G
Cuts at: 131 1767 131
Size: 1636 3193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 741 2779 2960 3123 741
Size: 2038 181 163 2447
Fragments arranged by size:
2447 2038 181 163
BtsI GCAGTG_nn'
Cuts at: 252 2072 252
Size: 1820 3009
DraI TTT'AAA
Cuts at: 1828 1828
Size: 4829
DrdI GACnn_nn'nnGTC
Cuts at: 2535 3393 2535
Size: 858 3971
EaeI y'GGCC_r
Cuts at: 981 2414 2588 2979 3006 4351 981
Size: 1433 174 391 27 1345 1459
Fragments arranged by size:
1459 1433 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2414 2414
Size: 4829
EarI CTCTTCn'nnn_
Cuts at: 211 1501 2852 3062 211
Size: 1290 1351 210 1978
Fragments arranged by size:
1978 1351 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3037 3347 3493 4639 3037
Size: 310 146 1146 3227
Fragments arranged by size:
3227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1841 1841
Size: 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 176 266 367 461 755 1322 1346 1358
Size: 3 90 101 94 294 567 24 12
Cuts at: 1358 1590 2654 3086 3833 4101 173
Size: 232 1064 432 747 268 901
Fragments arranged by size:
1064 901 747 567 432 294 268 232
101 94 90 24 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 395 1133 395
Size: 738 4091
EcoO109I rG'GnC_Cy
Cuts at: 1751 1968 1751
Size: 217 4612
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 728 810 2848 728
Size: 82 2038 2709
Fragments arranged by size:
2709 2038 82
FspI TGC'GCA
Cuts at: 2610 2610
Size: 4829
HaeII r_GCGC'y
Cuts at: 168 2263 2511 3533 4512 168
Size: 2095 248 1022 979 485
Fragments arranged by size:
2095 1022 979 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 961 993 1389 1421 2671 2701 2703 2733 961
Size: 32 396 32 1250 30 2 30 3057
Fragments arranged by size:
3057 1250 396 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1868 1868
Size: 4829
HindIII A'AGCT_T
Cuts at: 379 989 1054 379
Size: 610 65 4154
Fragments arranged by size:
4154 610 65
KasI G'GCGC_C
Cuts at: 164 2259 2507 164
Size: 2095 248 2486
Fragments arranged by size:
2486 2095 248
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 733 3500 3684 733
Size: 2767 184 1878
Fragments arranged by size:
2767 1878 184
MscI TGG'CCA
Cuts at: 2590 2590
Size: 4829
MslI CAynn'nnrTG
Cuts at: 334 1579 2818 2945 334
Size: 1245 1239 127 2218
Fragments arranged by size:
2218 1245 1239 127
MspA1I CmG'CkG
Cuts at: 133 220 274 775 1285 1940 2254 2614 3627
Size: 87 54 501 510 655 314 360 1013
Cuts at: 3627 3872 133
Size: 245 1090
Fragments arranged by size:
1090 1013 655 510 501 360 314 245
87 54
NaeI GCC'GGC
Cuts at: 1313 3010 1313
Size: 1697 3132
NarI GG'CG_CC
Cuts at: 165 2260 2508 165
Size: 2095 248 2486
Fragments arranged by size:
2486 2095 248
NcoI C'CATG_G
Cuts at: 1767 1767
Size: 4829
NdeI CA'TA_TG
Cuts at: 1580 1580
Size: 4829
NgoMIV G'CCGG_C
Cuts at: 1311 3008 1311
Size: 1697 3132
NheI G'CTAG_C
Cuts at: 1378 1378
Size: 4829
NsiI A_TGCA'T
Cuts at: 812 812
Size: 4829
NspI r_CATG'y
Cuts at: 473 850 1882 2382 3289 473
Size: 377 1032 500 907 2013
Fragments arranged by size:
2013 1032 907 500 377
PciI A'CATG_T
Cuts at: 469 3285 469
Size: 2816 2013
PflMI CCAn_nnn'nTGG
Cuts at: 979 1198 1711 979
Size: 219 513 4097
Fragments arranged by size:
4097 513 219
PfoI T'CCnGG_A
Cuts at: 150 150
Size: 4829
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PpuMI rG'GwC_Cy
Cuts at: 1751 1751
Size: 4829
PspXI vC'TCGA_Gb
Cuts at: 488 542 488
Size: 54 4775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 836 868 836
Size: 32 4797
PstI C_TGCA'G
Cuts at: 534 1876 2561 534
Size: 1342 685 2802
Fragments arranged by size:
2802 1342 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4829
PvuII CAG'CTG
Cuts at: 274 775 1285 2254 2614 274
Size: 501 510 969 360 2489
Fragments arranged by size:
2489 969 510 501 360
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 1843 1843
Size: 4829
SacII CC_GC'GG
Cuts at: 134 134
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 4346 4346
Size: 4829
SexAI A'CCwGG_T
Cuts at: 428 428
Size: 4829
SfcI C'TryA_G
Cuts at: 33 530 1872 2557 3550 3741 33
Size: 497 1342 685 993 191 1121
Fragments arranged by size:
1342 1121 993 685 497 191
SfoI GGC'GCC
Cuts at: 166 2261 2509 166
Size: 2095 248 2486
Fragments arranged by size:
2486 2095 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 1851 1851
Size: 4829
SmlI C'TyrA_G
Cuts at: 488 500 542 1984 2899 3391 3653 488
Size: 12 42 1442 915 492 262 1664
Fragments arranged by size:
1664 1442 915 492 262 42 12
SnaBI TAC'GTA
Cuts at: 1821 1821
Size: 4829
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1882 2382 1882
Size: 500 4329
StuI AGG'CCT
Cuts at: 401 1037 401
Size: 636 4193
StyI C'CwwG_G
Cuts at: 290 1767 1963 2317 290
Size: 1477 196 354 2802
Fragments arranged by size:
2802 1477 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 1390 2412 2545 3004 3213 768
Size: 622 1022 133 459 209 2384
Fragments arranged by size:
2384 1022 622 459 209 133
TatI w'GTAC_w
Cuts at: 4344 4344
Size: 4829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 261 293 1755 1787 4346 4378 191
Size: 32 38 32 1462 32 2559 32 642
Fragments arranged by size:
2559 1462 642 38 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1391 2626 1391
Size: 1235 3594
XbaI T'CTAG_A
Cuts at: 840 1096 1860 840
Size: 256 764 3809
Fragments arranged by size:
3809 764 256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2324
Size: 4829
XhoI C'TCGA_G
Cuts at: 488 542 488
Size: 54 4775
XmaI C'CCGG_G
Cuts at: 1849 1849
Size: 4829
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BglI BmgBI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NotI NruI PacI PasI PmlI PshAI
PsiI PspOMI SanDI SfiI SgrAI SrfI SspI SwaI
XmnI ZraI