Restriction map for
pF1KSDA0908
(Circular) (Six-base) MAPSORT of: pF1KSDA0908.seq Check: 1667 from: 1 to: 4654
pF1KSDA0908 4654 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1013 1690 1013
Size: 677 3977
Acc65I G'GTAC_C
Cuts at: 1668 1668
Size: 4654
AclI AA'CG_TT
Cuts at: 426 4494 426
Size: 4068 586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 636 2477 2909 3658 3926 251
Size: 385 1841 432 749 268 979
Fragments arranged by size:
1841 979 749 432 385 268
AflIII A'CryG_T
Cuts at: 584 599 2004 3110 4087 584
Size: 15 1405 1106 977 1151
Fragments arranged by size:
1405 1151 1106 977 15
AgeI A'CCGG_T
Cuts at: 3042 3042
Size: 4654
AlwNI CAG_nnn'CTG
Cuts at: 1957 3526 1957
Size: 1569 3085
ApaLI G'TGCA_C
Cuts at: 3424 3424
Size: 4654
ApoI r'AATT_y
Cuts at: 292 1129 1216 1376 1656 4007 4263 292
Size: 837 87 160 280 2351 256 683
Fragments arranged by size:
2351 837 683 280 256 160 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 4654
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4654
AvaI C'yCGr_G
Cuts at: 199 1672 199
Size: 1473 3181
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4654
BanI G'GyrC_C
Cuts at: 205 1668 2082 2330 2365 205
Size: 1463 414 248 35 2494
Fragments arranged by size:
2494 1463 414 248 35
BanII G_rGCy'C
Cuts at: 149 1666 2696 149
Size: 1517 1030 2107
Fragments arranged by size:
2107 1517 1030
BbeI G_GCGC'C
Cuts at: 209 2086 2334 209
Size: 1877 248 2529
Fragments arranged by size:
2529 1877 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 233 199
Size: 34 4620
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3313 4654 2550
Size: 763 1341 2550
Fragments arranged by size:
2550 1341 763
BclI T'GATC_A
Cuts at: 1602 2172 1602
Size: 570 4084
BglI GCCn_nnn'nGGC
Cuts at: 203 203
Size: 4654
BglII A'GATC_T
Cuts at: 1343 2167 4206 1343
Size: 824 2039 1791
Fragments arranged by size:
2039 1791 824
BlpI GC'TnA_GC
Cuts at: 1764 1764
Size: 4654
Bme1580I G_kGCm'C
Cuts at: 1286 1589 2277 2370 3428 1286
Size: 303 688 93 1058 2512
Fragments arranged by size:
2512 1058 688 303 93
BmgBI CAC'GTC
Cuts at: 600 600
Size: 4654
BmrI ACTGGGnnnn_n'
Cuts at: 487 1864 1945 2280 4426 487
Size: 1377 81 335 2146 715
Fragments arranged by size:
2146 1377 715 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 3019 168
Size: 2851 1803
Bpu10I CC'TnA_GC
Cuts at: 900 1310 900
Size: 410 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1828 2707 3201 3499 1828
Size: 879 494 298 2983
Fragments arranged by size:
2983 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 451 4358 451
Size: 3907 747
BsaAI yAC'GTr
Cuts at: 1644 2005 1644
Size: 361 4293
BsaBI GATnn'nnATC
Cuts at: 6 2166 4205 6
Size: 2160 2039 455
Fragments arranged by size:
2160 2039 455
BsaHI Gr'CG_yC
Cuts at: 206 2083 2331 3035 4531 206
Size: 1877 248 704 1496 329
Fragments arranged by size:
1877 1496 704 329 248
BsaWI w'CCGG_w
Cuts at: 1841 2362 3042 3316 3463 3874 1841
Size: 521 680 274 147 411 2621
Fragments arranged by size:
2621 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 380
Size: 30 4624
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 526 701 973 144
Size: 382 175 272 3825
Fragments arranged by size:
3825 382 272 175
BseYI C'CCAG_C
Cuts at: 162 2077 2835 3414 3910 162
Size: 1915 758 579 496 906
Fragments arranged by size:
1915 906 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 385 400 1532 1541 204
Size: 181 15 1132 9 3317
Fragments arranged by size:
3317 1132 181 15 9
BsiEI CG_ry'CG
Cuts at: 104 2194 2240 3450 104
Size: 2090 46 1210 1308
Fragments arranged by size:
2090 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 149 222 1666 2444 2634 3428 149
Size: 73 1444 778 190 794 1375
Fragments arranged by size:
1444 1375 794 778 190 73
BsmI GAATG_Cn'
Cuts at: 710 710
Size: 4654
BsmBI CGTCTCn'nnnn_
Cuts at: 156 156
Size: 4654
Bsp1286I G_dGCh'C
Cuts at: 149 222 1286 1589 1666 2277 2370 2444 2634
Size: 73 1064 303 77 611 93 74 190
Cuts at: 2634 2696 3428 149
Size: 62 732 1375
Fragments arranged by size:
1375 1064 732 611 303 190 93 77
74 73 62
BspEI T'CCGG_A
Cuts at: 3874 3874
Size: 4654
BspHI T'CATG_A
Cuts at: 614 639 3830 614
Size: 25 3191 1438
Fragments arranged by size:
3191 1438 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 1702 1916 2218 2599 290
Size: 1412 214 302 381 2345
Fragments arranged by size:
2345 1412 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2944 2998 4484 4649 50
Size: 2894 54 1486 165 55
Fragments arranged by size:
2894 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1538 2564 1538
Size: 1026 3628
BsrFI r'CCGG_y
Cuts at: 742 2650 2831 3042 742
Size: 1908 181 211 2354
Fragments arranged by size:
2354 1908 211 181
BsrGI T'GTAC_A
Cuts at: 950 950
Size: 4654
BssSI C'ACGA_G
Cuts at: 2923 3283 2923
Size: 360 4294
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1564 2029 1519
Size: 45 465 4144
Fragments arranged by size:
4144 465 45
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4654
BstYI r'GATC_y
Cuts at: 1 620 1305 1343 1677 2144 2167 2502 2748
Size: 619 685 38 334 467 23 335 246
Cuts at: 2748 3762 3848 3860 4206 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 685 619 467 449 346 335 334
246 86 38 23 12
BtgI C'CryG_G
Cuts at: 125 1420 1523 125
Size: 1295 103 3256
Fragments arranged by size:
3256 1295 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2602 2783 2946 2602
Size: 181 163 4310
Fragments arranged by size:
4310 181 163
BtsI GCAGTG_nn'
Cuts at: 1895 1895
Size: 4654
DraI TTT'AAA
Cuts at: 410 1382 1651 410
Size: 972 269 3413
Fragments arranged by size:
3413 972 269
DrdI GACnn_nn'nnGTC
Cuts at: 2358 3218 2358
Size: 860 3794
EaeI y'GGCC_r
Cuts at: 801 1064 2237 2411 2802 2829 4176 801
Size: 263 1173 174 391 27 1347 1279
Fragments arranged by size:
1347 1279 1173 391 263 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4654
EarI CTCTTCn'nnn_
Cuts at: 326 2675 2885 326
Size: 2349 210 2095
Fragments arranged by size:
2349 2095 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 212 2860 3172 3318 4464 212
Size: 2648 312 146 1146 402
Fragments arranged by size:
2648 1146 402 312 146
EcoICRI GAG'CTC
Cuts at: 147 1664 147
Size: 1517 3137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 636 2477 2909 3658 3926 251
Size: 385 1841 432 749 268 979
Fragments arranged by size:
1841 979 749 432 385 268
EcoO109I rG'GnC_Cy
Cuts at: 1791 1791
Size: 4654
EcoRI G'AATT_C
Cuts at: 1129 1656 1129
Size: 527 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 762 1378 2671 762
Size: 616 1293 2745
Fragments arranged by size:
2745 1293 616
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4654
HaeII r_GCGC'y
Cuts at: 209 558 2086 2334 3358 4337 209
Size: 349 1528 248 1024 979 526
Fragments arranged by size:
1528 1024 979 526 349 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 380 412 2494 2524 2526 2556 320
Size: 32 28 32 2082 30 2 30 2418
Fragments arranged by size:
2418 2082 32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4654
KasI G'GCGC_C
Cuts at: 205 2082 2330 205
Size: 1877 248 2529
Fragments arranged by size:
2529 1877 248
KpnI G_GTAC'C
Cuts at: 1672 1672
Size: 4654
MfeI C'AATT_G
Cuts at: 1121 1121
Size: 4654
MluI A'CGCG_T
Cuts at: 4087 4087
Size: 4654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1015 3325 3509 1015
Size: 2310 184 2160
Fragments arranged by size:
2310 2160 184
MscI TGG'CCA
Cuts at: 803 1066 2413 803
Size: 263 1347 3044
Fragments arranged by size:
3044 1347 263
MslI CAynn'nnrTG
Cuts at: 361 644 1528 1594 2641 2768 361
Size: 283 884 66 1047 127 2247
Fragments arranged by size:
2247 1047 884 283 127 66
MspA1I CmG'CkG
Cuts at: 188 1577 1763 2077 2437 3452 3697 188
Size: 1389 186 314 360 1015 245 1145
Fragments arranged by size:
1389 1145 1015 360 314 245 186
NaeI GCC'GGC
Cuts at: 744 2833 744
Size: 2089 2565
NarI GG'CG_CC
Cuts at: 206 2083 2331 206
Size: 1877 248 2529
Fragments arranged by size:
2529 1877 248
NcoI C'CATG_G
Cuts at: 125 1420 1523 125
Size: 1295 103 3256
Fragments arranged by size:
3256 1295 103
NdeI CA'TA_TG
Cuts at: 1332 1332
Size: 4654
NgoMIV G'CCGG_C
Cuts at: 742 2831 742
Size: 2089 2565
NruI TCG'CGA
Cuts at: 238 3015 238
Size: 2777 1877
NspI r_CATG'y
Cuts at: 588 1705 2205 3114 588
Size: 1117 500 909 2128
Fragments arranged by size:
2128 1117 909 500
PciI A'CATG_T
Cuts at: 584 3110 584
Size: 2526 2128
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1017 1049 3793 3825 1017
Size: 32 2744 32 1846
Fragments arranged by size:
2744 1846 32 32
PshAI GACnn'nnGTC
Cuts at: 443 443
Size: 4654
PstI C_TGCA'G
Cuts at: 914 1699 2384 914
Size: 785 685 3184
Fragments arranged by size:
3184 785 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4654
PvuII CAG'CTG
Cuts at: 188 2077 2437 188
Size: 1889 360 2405
Fragments arranged by size:
2405 1889 360
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4654
SacI G_AGCT'C
Cuts at: 149 1666 149
Size: 1517 3137
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4654
SapI GCTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4444
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4654
ScaI AGT'ACT
Cuts at: 4171 4171
Size: 4654
SfcI C'TryA_G
Cuts at: 33 910 1695 2380 3375 3566 33
Size: 877 785 685 995 191 1121
Fragments arranged by size:
1121 995 877 785 685 191
SfoI GGC'GCC
Cuts at: 207 2084 2332 207
Size: 1877 248 2529
Fragments arranged by size:
2529 1877 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4654
SmaI CCC'GGG
Cuts at: 1674 1674
Size: 4654
SmlI C'TyrA_G
Cuts at: 1807 2722 3216 3478 1807
Size: 915 494 262 2983
Fragments arranged by size:
2983 915 494 262
SnaBI TAC'GTA
Cuts at: 1644 1644
Size: 4654
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4654
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4154
SspI AAT'ATT
Cuts at: 1095 1141 1095
Size: 46 4608
StuI AGG'CCT
Cuts at: 463 463
Size: 4654
StyI C'CwwG_G
Cuts at: 125 699 1420 1523 1786 2140 125
Size: 574 721 103 263 354 2639
Fragments arranged by size:
2639 721 574 354 263 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2235 2368 2827 3038 2235
Size: 133 459 211 3851
Fragments arranged by size:
3851 459 211 133
TatI w'GTAC_w
Cuts at: 950 977 4169 950
Size: 27 3192 1435
Fragments arranged by size:
3192 1435 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 972 1004 4171 4203 972
Size: 32 3167 32 1423
Fragments arranged by size:
3167 1423 32 32
Tth111I GACn'n_nGTC
Cuts at: 1054 2449 1054
Size: 1395 3259
XbaI T'CTAG_A
Cuts at: 1201 1683 1201
Size: 482 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 2147
Size: 4654
XmaI C'CCGG_G
Cuts at: 1672 1672
Size: 4654
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
BpmI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NheI NotI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI XmnI ZraI