(Circular) (Six-base) MAPSORT of: pF1KSDA0915.seq Check: 1373 from: 1 to: 5662 pF1KSDA0915 5662 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2439 2489 2439 Size: 50 5612 AatII G_ACGT'C Cuts at: 1408 1408 Size: 5662 AccI GT'mk_AC Cuts at: 2698 2698 Size: 5662 Acc65I G'GTAC_C Cuts at: 2676 2676 Size: 5662 AclI AA'CG_TT Cuts at: 5502 5502 Size: 5662 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 629 1061 1159 1970 2462 2516 2527 2606 3485 Size: 432 98 811 492 54 11 79 879 Cuts at: 3485 3917 4666 4934 629 Size: 432 749 268 1357 Fragments arranged by size: 1357 879 811 749 492 432 432 268 98 79 54 11 AfeI AGC'GCT Cuts at: 1923 2326 1923 Size: 403 5259 AflIII A'CryG_T Cuts at: 3012 4118 5095 3012 Size: 1106 977 3579 Fragments arranged by size: 3579 1106 977 AgeI A'CCGG_T Cuts at: 4050 4050 Size: 5662 AleI CACnn'nnGTG Cuts at: 1453 1708 1453 Size: 255 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 532 964 1840 532 Size: 432 876 4354 Fragments arranged by size: 4354 876 432 AlwNI CAG_nnn'CTG Cuts at: 460 565 730 1102 2178 2409 2965 4534 460 Size: 105 165 372 1076 231 556 1569 1588 Fragments arranged by size: 1588 1569 1076 556 372 231 165 105 ApaLI G'TGCA_C Cuts at: 297 4432 297 Size: 4135 1527 ApoI r'AATT_y Cuts at: 2032 2664 5015 5271 2032 Size: 632 2351 256 2423 Fragments arranged by size: 2423 2351 632 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5662 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5662 AvaI C'yCGr_G Cuts at: 2602 2680 2602 Size: 78 5584 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1165 1198 1165 Size: 33 5629 BamHI G'GATC_C Cuts at: 2223 2685 2223 Size: 462 5200 BanI G'GyrC_C Cuts at: 801 2024 2060 2576 2626 2676 3090 3338 3373 Size: 1223 36 516 50 50 414 248 35 Cuts at: 3373 801 Size: 3090 Fragments arranged by size: 3090 1223 516 414 248 50 50 36 35 BanII G_rGCy'C Cuts at: 320 621 794 2674 3704 320 Size: 301 173 1880 1030 2278 Fragments arranged by size: 2278 1880 1030 301 173 BbeI G_GCGC'C Cuts at: 2028 3094 3342 2028 Size: 1066 248 4348 Fragments arranged by size: 4348 1066 248 BbsI GAAGACnn'nnnn_ Cuts at: 1336 1336 Size: 5662 BbvCI CC'TCA_GC Cuts at: 360 558 1357 1925 360 Size: 198 799 568 4097 Fragments arranged by size: 4097 799 568 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1890 1924 1890 Size: 34 5628 BciVI GTATCCnnnnn_n' Cuts at: 1344 3558 4321 5662 1344 Size: 2214 763 1341 1344 Fragments arranged by size: 2214 1344 1341 763 BclI T'GATC_A Cuts at: 1494 3180 1494 Size: 1686 3976 BglI GCCn_nnn'nGGC Cuts at: 1882 1882 Size: 5662 BglII A'GATC_T Cuts at: 1023 3175 5214 1023 Size: 2152 2039 1471 Fragments arranged by size: 2152 2039 1471 BlpI GC'TnA_GC Cuts at: 935 1459 2772 935 Size: 524 1313 3825 Fragments arranged by size: 3825 1313 524 Bme1580I G_kGCm'C Cuts at: 301 3285 3378 4436 301 Size: 2984 93 1058 1527 Fragments arranged by size: 2984 1527 1058 93 BmrI ACTGGGnnnn_n' Cuts at: 129 213 2872 2953 3288 5434 129 Size: 84 2659 81 335 2146 357 Fragments arranged by size: 2659 2146 357 335 84 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 394 701 1265 4027 394 Size: 307 564 2762 2029 Fragments arranged by size: 2762 2029 564 307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 458 551 707 808 1079 1235 1283 2476 458 Size: 93 156 101 271 156 48 1193 3644 Fragments arranged by size: 3644 1193 271 156 156 101 93 48 Bpu10I CC'TnA_GC Cuts at: 360 558 620 1357 1925 360 Size: 198 62 737 568 4097 Fragments arranged by size: 4097 737 568 198 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 319 943 2836 3715 4209 4507 319 Size: 624 1893 879 494 298 1474 Fragments arranged by size: 1893 1474 879 624 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 476 652 1675 2016 5366 476 Size: 176 1023 341 3350 772 Fragments arranged by size: 3350 1023 772 341 176 BsaAI yAC'GTr Cuts at: 2652 3013 2652 Size: 361 5301 BsaBI GATnn'nnATC Cuts at: 6 1028 3174 5213 6 Size: 1022 2146 2039 455 Fragments arranged by size: 2146 2039 1022 455 BsaHI Gr'CG_yC Cuts at: 1405 2025 3091 3339 4043 5539 1405 Size: 620 1066 248 704 1496 1528 Fragments arranged by size: 1528 1496 1066 704 620 248 BsaWI w'CCGG_w Cuts at: 2220 2849 3370 4050 4324 4471 4882 2220 Size: 629 521 680 274 147 411 3000 Fragments arranged by size: 3000 680 629 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 229 259 1411 1441 229 Size: 30 1152 30 4450 Fragments arranged by size: 4450 1152 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 237 835 946 1070 1082 1135 1228 1693 2586 Size: 598 111 124 12 53 93 465 893 Cuts at: 2586 237 Size: 3313 Fragments arranged by size: 3313 893 598 465 124 111 93 53 12 BseYI C'CCAG_C Cuts at: 321 2210 2331 2534 3085 3843 4422 4918 321 Size: 1889 121 203 551 758 579 496 1065 Fragments arranged by size: 1889 1065 758 579 551 496 203 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 492 1540 2411 2467 492 Size: 1048 871 56 3687 Fragments arranged by size: 3687 1048 871 56 BsiEI CG_ry'CG Cuts at: 104 3202 3248 4458 104 Size: 3098 46 1210 1308 Fragments arranged by size: 3098 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 301 723 794 2081 2674 3452 3642 4436 301 Size: 422 71 1287 593 778 190 794 1527 Fragments arranged by size: 1527 1287 794 778 593 422 190 71 BsmI GAATG_Cn' Cuts at: 1878 1878 Size: 5662 Bsp1286I G_dGCh'C Cuts at: 301 320 621 723 794 2081 2674 3285 3378 Size: 19 301 102 71 1287 593 611 93 Cuts at: 3378 3452 3642 3704 4436 301 Size: 74 190 62 732 1527 Fragments arranged by size: 1527 1287 732 611 593 301 190 102 93 74 71 62 19 BspEI T'CCGG_A Cuts at: 2220 4882 2220 Size: 2662 3000 BspHI T'CATG_A Cuts at: 4838 4838 Size: 5662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 468 581 1430 2141 2205 2439 2489 2710 2924 Size: 113 849 711 64 234 50 221 214 Cuts at: 2924 3226 3607 468 Size: 302 381 2523 Fragments arranged by size: 2523 849 711 381 302 234 221 214 113 64 50 BsrBI CCG'CTC Cuts at: 50 926 1545 1761 3952 4006 5492 5657 50 Size: 876 619 216 2191 54 1486 165 55 Fragments arranged by size: 2191 1486 876 619 216 165 55 54 BsrDI GCAATG_nn' Cuts at: 3572 3572 Size: 5662 BsrFI r'CCGG_y Cuts at: 1733 3658 3839 4050 1733 Size: 1925 181 211 3345 Fragments arranged by size: 3345 1925 211 181 BssSI C'ACGA_G Cuts at: 977 3931 4291 977 Size: 2954 360 2348 Fragments arranged by size: 2954 2348 360 BstAPI GCAn_nnn'nTGC Cuts at: 3037 3037 Size: 5662 BstBI TT'CG_AA Cuts at: 110 110 Size: 5662 BstEII G'GTnAC_C Cuts at: 500 895 500 Size: 395 5267 BstYI r'GATC_y Cuts at: 1 1023 2223 2685 3152 3175 3510 3756 4770 Size: 1022 1200 462 467 23 335 246 1014 Cuts at: 4770 4856 4868 5214 1 Size: 86 12 346 449 Fragments arranged by size: 1200 1022 1014 467 462 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 524 524 Size: 5662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2176 3610 3791 3954 2176 Size: 1434 181 163 3884 Fragments arranged by size: 3884 1434 181 163 BtsI GCAGTG_nn' Cuts at: 929 2903 929 Size: 1974 3688 DraI TTT'AAA Cuts at: 2659 2659 Size: 5662 DraIII CAC_nnn'GTG Cuts at: 1310 1626 1310 Size: 316 5346 DrdI GACnn_nn'nnGTC Cuts at: 3366 4226 3366 Size: 860 4802 EaeI y'GGCC_r Cuts at: 3245 3419 3810 3837 5184 3245 Size: 174 391 27 1347 3723 Fragments arranged by size: 3723 1347 391 174 27 EagI C'GGCC_G Cuts at: 3245 3245 Size: 5662 EarI CTCTTCn'nnn_ Cuts at: 1112 1513 1584 1844 1946 3683 3893 1112 Size: 401 71 260 102 1737 210 2881 Fragments arranged by size: 2881 1737 401 260 210 102 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 175 435 1710 2236 3868 4180 4326 5472 175 Size: 260 1275 526 1632 312 146 1146 365 Fragments arranged by size: 1632 1275 1146 526 365 312 260 146 EcoICRI GAG'CTC Cuts at: 792 2672 792 Size: 1880 3782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 458 551 629 707 808 1061 1079 1159 1235 Size: 93 78 78 101 253 18 80 76 Cuts at: 1235 1283 1970 2462 2476 2516 2527 2606 3485 Size: 48 687 492 14 40 11 79 879 Cuts at: 3485 3917 4666 4934 458 Size: 432 749 268 1186 Fragments arranged by size: 1186 879 749 687 492 432 268 253 101 93 80 79 78 78 76 48 40 18 14 11 EcoNI CCTnn'n_nnAGG Cuts at: 1312 2203 2621 1312 Size: 891 418 4353 Fragments arranged by size: 4353 891 418 EcoO109I rG'GnC_Cy Cuts at: 229 704 825 834 1008 1630 1884 1998 2353 Size: 475 121 9 174 622 254 114 355 Cuts at: 2353 2522 2799 229 Size: 169 277 3092 Fragments arranged by size: 3092 622 475 355 277 254 174 169 121 114 9 EcoRI G'AATT_C Cuts at: 2032 2664 2032 Size: 632 5030 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3679 3679 Size: 5662 FspI TGC'GCA Cuts at: 1816 3441 1816 Size: 1625 4037 HaeII r_GCGC'y Cuts at: 444 1925 2028 2328 3094 3342 4366 5345 444 Size: 1481 103 300 766 248 1024 979 761 Fragments arranged by size: 1481 1024 979 766 761 300 248 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 3502 3532 3534 3564 3502 Size: 30 2 30 5600 Fragments arranged by size: 5600 30 30 2 HincII GTy'rAC Cuts at: 2699 2699 Size: 5662 HindIII A'AGCT_T Cuts at: 2300 2300 Size: 5662 KasI G'GCGC_C Cuts at: 2024 3090 3338 2024 Size: 1066 248 4348 Fragments arranged by size: 4348 1066 248 KpnI G_GTAC'C Cuts at: 2680 2680 Size: 5662 MluI A'CGCG_T Cuts at: 5095 5095 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2592 4333 4517 2592 Size: 1741 184 3737 Fragments arranged by size: 3737 1741 184 MscI TGG'CCA Cuts at: 3421 3421 Size: 5662 MslI CAynn'nnrTG Cuts at: 1453 1708 3649 3776 1453 Size: 255 1941 127 3339 Fragments arranged by size: 3339 1941 255 127 MspA1I CmG'CkG Cuts at: 325 855 1340 1770 2166 2538 2771 3085 3445 Size: 530 485 430 396 372 233 314 360 Cuts at: 3445 4460 4705 325 Size: 1015 245 1282 Fragments arranged by size: 1282 1015 530 485 430 396 372 360 314 245 233 NaeI GCC'GGC Cuts at: 1735 3841 1735 Size: 2106 3556 NarI GG'CG_CC Cuts at: 2025 3091 3339 2025 Size: 1066 248 4348 Fragments arranged by size: 4348 1066 248 NcoI C'CATG_G Cuts at: 524 524 Size: 5662 NgoMIV G'CCGG_C Cuts at: 1733 3839 1733 Size: 2106 3556 NruI TCG'CGA Cuts at: 4023 4023 Size: 5662 NspI r_CATG'y Cuts at: 1380 1618 1821 2322 2713 3213 4122 1380 Size: 238 203 501 391 500 909 2920 Fragments arranged by size: 2920 909 501 500 391 238 203 PasI CC'CwG_GG Cuts at: 226 830 1887 226 Size: 604 1057 4001 Fragments arranged by size: 4001 1057 604 PciI A'CATG_T Cuts at: 4118 4118 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 311 730 1626 311 Size: 419 896 4347 Fragments arranged by size: 4347 896 419 PfoI T'CCnGG_A Cuts at: 1472 1472 Size: 5662 PmeI GTTT'AAAC Cuts at: 2659 2659 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4801 4833 4801 Size: 32 5630 PpuMI rG'GwC_Cy Cuts at: 704 825 834 1008 704 Size: 121 9 174 5358 Fragments arranged by size: 5358 174 121 9 PstI C_TGCA'G Cuts at: 150 1169 1742 1829 2707 3392 150 Size: 1019 573 87 878 685 2420 Fragments arranged by size: 2420 1019 878 685 573 87 PvuI CG_AT'CG Cuts at: 104 104 Size: 5662 PvuII CAG'CTG Cuts at: 325 2538 3085 3445 325 Size: 2213 547 360 2542 Fragments arranged by size: 2542 2213 547 360 RsrII CG'GwC_CG Cuts at: 3855 3855 Size: 5662 SacI G_AGCT'C Cuts at: 794 2674 794 Size: 1880 3782 SalI G'TCGA_C Cuts at: 2697 2697 Size: 5662 SanDI GG'GwC_CC Cuts at: 704 834 1008 704 Size: 130 174 5358 Fragments arranged by size: 5358 174 130 SapI GCTCTTCn'nnn_ Cuts at: 1584 1946 3683 3893 1584 Size: 362 1737 210 3353 Fragments arranged by size: 3353 1737 362 210 SbfI CC_TGCA'GG Cuts at: 1169 2707 1169 Size: 1538 4124 ScaI AGT'ACT Cuts at: 5179 5179 Size: 5662 SfcI C'TryA_G Cuts at: 33 146 1165 1686 1738 1825 2703 3388 4383 Size: 113 1019 521 52 87 878 685 995 Cuts at: 4383 4574 33 Size: 191 1121 Fragments arranged by size: 1121 1019 995 878 685 521 191 113 87 52 SfoI GGC'GCC Cuts at: 2026 3092 3340 2026 Size: 1066 248 4348 Fragments arranged by size: 4348 1066 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5662 SmaI CCC'GGG Cuts at: 2682 2682 Size: 5662 SmlI C'TyrA_G Cuts at: 334 958 2602 2815 3730 4224 4486 334 Size: 624 1644 213 915 494 262 1510 Fragments arranged by size: 1644 1510 915 624 494 262 213 SnaBI TAC'GTA Cuts at: 2652 2652 Size: 5662 SpeI A'CTAG_T Cuts at: 67 67 Size: 5662 SphI G_CATG'C Cuts at: 1380 1618 1821 2713 3213 1380 Size: 238 203 892 500 3829 Fragments arranged by size: 3829 892 500 238 203 StyI C'CwwG_G Cuts at: 524 2794 3148 524 Size: 2270 354 3038 Fragments arranged by size: 3038 2270 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 501 789 883 1219 1287 1459 1879 1984 2299 Size: 288 94 336 68 172 420 105 315 Cuts at: 2299 3243 3376 3835 4046 501 Size: 944 133 459 211 2117 Fragments arranged by size: 2117 944 459 420 336 315 288 211 172 133 105 94 68 TatI w'GTAC_w Cuts at: 5177 5177 Size: 5662 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 229 261 411 443 1262 1294 5179 5211 229 Size: 32 150 32 819 32 3885 32 680 Fragments arranged by size: 3885 819 680 150 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2450 3457 2450 Size: 1007 4655 XbaI T'CTAG_A Cuts at: 367 2691 367 Size: 2324 3338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1344 3155 1344 Size: 1811 3851 XhoI C'TCGA_G Cuts at: 2602 2602 Size: 5662 XmaI C'CCGG_G Cuts at: 2680 2680 Size: 5662 ZraI GAC'GTC Cuts at: 1406 1406 Size: 5662 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AflII AhdI AloI ApaI AscI AvrII BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI StuI SwaI XmnI