Restriction map for pF1KSDA0915
 (Circular) (Six-base) MAPSORT of: pF1KSDA0915.seq  Check: 1373  from: 1  to: 5662



pF1KSDA0915  5662 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2439    2489    2439 
   Size:         50    5612
 
AatII G_ACGT'C

Cuts at:   1408    1408 
   Size:       5662
 
AccI GT'mk_AC

Cuts at:   2698    2698 
   Size:       5662
 
Acc65I G'GTAC_C

Cuts at:   2676    2676 
   Size:       5662
 
AclI AA'CG_TT

Cuts at:   5502    5502 
   Size:       5662
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    629    1061    1159    1970    2462    2516    2527    2606    3485 
   Size:        432      98     811     492      54      11      79     879

Cuts at:   3485    3917    4666    4934     629 
   Size:        432     749     268    1357

  Fragments arranged by size: 

                1357     879     811     749     492     432     432     268
                  98      79      54      11
 
AfeI AGC'GCT

Cuts at:   1923    2326    1923 
   Size:        403    5259
 
AflIII A'CryG_T

Cuts at:   3012    4118    5095    3012 
   Size:       1106     977    3579

  Fragments arranged by size: 

                3579    1106     977
 
AgeI A'CCGG_T

Cuts at:   4050    4050 
   Size:       5662
 
AleI CACnn'nnGTG

Cuts at:   1453    1708    1453 
   Size:        255    5407
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    532     964    1840     532 
   Size:        432     876    4354

  Fragments arranged by size: 

                4354     876     432
 
AlwNI CAG_nnn'CTG

Cuts at:    460     565     730    1102    2178    2409    2965    4534     460 
   Size:        105     165     372    1076     231     556    1569    1588

  Fragments arranged by size: 

                1588    1569    1076     556     372     231     165     105
            
 
ApaLI G'TGCA_C

Cuts at:    297    4432     297 
   Size:       4135    1527
 
ApoI r'AATT_y

Cuts at:   2032    2664    5015    5271    2032 
   Size:        632    2351     256    2423

  Fragments arranged by size: 

                2423    2351     632     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5662
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5662
 
AvaI C'yCGr_G

Cuts at:   2602    2680    2602 
   Size:         78    5584
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1165    1198    1165 
   Size:         33    5629
 
BamHI G'GATC_C

Cuts at:   2223    2685    2223 
   Size:        462    5200
 
BanI G'GyrC_C

Cuts at:    801    2024    2060    2576    2626    2676    3090    3338    3373 
   Size:       1223      36     516      50      50     414     248      35

Cuts at:   3373     801 
   Size:       3090

  Fragments arranged by size: 

                3090    1223     516     414     248      50      50      36
                  35
 
BanII G_rGCy'C

Cuts at:    320     621     794    2674    3704     320 
   Size:        301     173    1880    1030    2278

  Fragments arranged by size: 

                2278    1880    1030     301     173
 
BbeI G_GCGC'C

Cuts at:   2028    3094    3342    2028 
   Size:       1066     248    4348

  Fragments arranged by size: 

                4348    1066     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1336    1336 
   Size:       5662
 
BbvCI CC'TCA_GC

Cuts at:    360     558    1357    1925     360 
   Size:        198     799     568    4097

  Fragments arranged by size: 

                4097     799     568     198
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1890    1924    1890 
   Size:         34    5628
 
BciVI GTATCCnnnnn_n'

Cuts at:   1344    3558    4321    5662    1344 
   Size:       2214     763    1341    1344

  Fragments arranged by size: 

                2214    1344    1341     763
 
BclI T'GATC_A

Cuts at:   1494    3180    1494 
   Size:       1686    3976
 
BglI GCCn_nnn'nGGC

Cuts at:   1882    1882 
   Size:       5662
 
BglII A'GATC_T

Cuts at:   1023    3175    5214    1023 
   Size:       2152    2039    1471

  Fragments arranged by size: 

                2152    2039    1471
 
BlpI GC'TnA_GC

Cuts at:    935    1459    2772     935 
   Size:        524    1313    3825

  Fragments arranged by size: 

                3825    1313     524
 
Bme1580I G_kGCm'C

Cuts at:    301    3285    3378    4436     301 
   Size:       2984      93    1058    1527

  Fragments arranged by size: 

                2984    1527    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    129     213    2872    2953    3288    5434     129 
   Size:         84    2659      81     335    2146     357

  Fragments arranged by size: 

                2659    2146     357     335      84      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    394     701    1265    4027     394 
   Size:        307     564    2762    2029

  Fragments arranged by size: 

                2762    2029     564     307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    458     551     707     808    1079    1235    1283    2476     458 
   Size:         93     156     101     271     156      48    1193    3644

  Fragments arranged by size: 

                3644    1193     271     156     156     101      93      48
            
 
Bpu10I CC'TnA_GC

Cuts at:    360     558     620    1357    1925     360 
   Size:        198      62     737     568    4097

  Fragments arranged by size: 

                4097     737     568     198      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    319     943    2836    3715    4209    4507     319 
   Size:        624    1893     879     494     298    1474

  Fragments arranged by size: 

                1893    1474     879     624     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    476     652    1675    2016    5366     476 
   Size:        176    1023     341    3350     772

  Fragments arranged by size: 

                3350    1023     772     341     176
 
BsaAI yAC'GTr

Cuts at:   2652    3013    2652 
   Size:        361    5301
 
BsaBI GATnn'nnATC

Cuts at:      6    1028    3174    5213       6 
   Size:       1022    2146    2039     455

  Fragments arranged by size: 

                2146    2039    1022     455
 
BsaHI Gr'CG_yC

Cuts at:   1405    2025    3091    3339    4043    5539    1405 
   Size:        620    1066     248     704    1496    1528

  Fragments arranged by size: 

                1528    1496    1066     704     620     248
 
BsaWI w'CCGG_w

Cuts at:   2220    2849    3370    4050    4324    4471    4882    2220 
   Size:        629     521     680     274     147     411    3000

  Fragments arranged by size: 

                3000     680     629     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    229     259    1411    1441     229 
   Size:         30    1152      30    4450

  Fragments arranged by size: 

                4450    1152      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    237     835     946    1070    1082    1135    1228    1693    2586 
   Size:        598     111     124      12      53      93     465     893

Cuts at:   2586     237 
   Size:       3313

  Fragments arranged by size: 

                3313     893     598     465     124     111      93      53
                  12
 
BseYI C'CCAG_C

Cuts at:    321    2210    2331    2534    3085    3843    4422    4918     321 
   Size:       1889     121     203     551     758     579     496    1065

  Fragments arranged by size: 

                1889    1065     758     579     551     496     203     121
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    492    1540    2411    2467     492 
   Size:       1048     871      56    3687

  Fragments arranged by size: 

                3687    1048     871      56
 
BsiEI CG_ry'CG

Cuts at:    104    3202    3248    4458     104 
   Size:       3098      46    1210    1308

  Fragments arranged by size: 

                3098    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    301     723     794    2081    2674    3452    3642    4436     301 
   Size:        422      71    1287     593     778     190     794    1527

  Fragments arranged by size: 

                1527    1287     794     778     593     422     190      71
            
 
BsmI GAATG_Cn'

Cuts at:   1878    1878 
   Size:       5662
 
Bsp1286I G_dGCh'C

Cuts at:    301     320     621     723     794    2081    2674    3285    3378 
   Size:         19     301     102      71    1287     593     611      93

Cuts at:   3378    3452    3642    3704    4436     301 
   Size:         74     190      62     732    1527

  Fragments arranged by size: 

                1527    1287     732     611     593     301     190     102
                  93      74      71      62      19
 
BspEI T'CCGG_A

Cuts at:   2220    4882    2220 
   Size:       2662    3000
 
BspHI T'CATG_A

Cuts at:   4838    4838 
   Size:       5662
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    468     581    1430    2141    2205    2439    2489    2710    2924 
   Size:        113     849     711      64     234      50     221     214

Cuts at:   2924    3226    3607     468 
   Size:        302     381    2523

  Fragments arranged by size: 

                2523     849     711     381     302     234     221     214
                 113      64      50
 
BsrBI CCG'CTC

Cuts at:     50     926    1545    1761    3952    4006    5492    5657      50 
   Size:        876     619     216    2191      54    1486     165      55

  Fragments arranged by size: 

                2191    1486     876     619     216     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   3572    3572 
   Size:       5662
 
BsrFI r'CCGG_y

Cuts at:   1733    3658    3839    4050    1733 
   Size:       1925     181     211    3345

  Fragments arranged by size: 

                3345    1925     211     181
 
BssSI C'ACGA_G

Cuts at:    977    3931    4291     977 
   Size:       2954     360    2348

  Fragments arranged by size: 

                2954    2348     360
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3037    3037 
   Size:       5662
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       5662
 
BstEII G'GTnAC_C

Cuts at:    500     895     500 
   Size:        395    5267
 
BstYI r'GATC_y

Cuts at:      1    1023    2223    2685    3152    3175    3510    3756    4770 
   Size:       1022    1200     462     467      23     335     246    1014

Cuts at:   4770    4856    4868    5214       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1200    1022    1014     467     462     449     346     335
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    524     524 
   Size:       5662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2176    3610    3791    3954    2176 
   Size:       1434     181     163    3884

  Fragments arranged by size: 

                3884    1434     181     163
 
BtsI GCAGTG_nn'

Cuts at:    929    2903     929 
   Size:       1974    3688
 
DraI TTT'AAA

Cuts at:   2659    2659 
   Size:       5662
 
DraIII CAC_nnn'GTG

Cuts at:   1310    1626    1310 
   Size:        316    5346
 
DrdI GACnn_nn'nnGTC

Cuts at:   3366    4226    3366 
   Size:        860    4802
 
EaeI y'GGCC_r

Cuts at:   3245    3419    3810    3837    5184    3245 
   Size:        174     391      27    1347    3723

  Fragments arranged by size: 

                3723    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   3245    3245 
   Size:       5662
 
EarI CTCTTCn'nnn_

Cuts at:   1112    1513    1584    1844    1946    3683    3893    1112 
   Size:        401      71     260     102    1737     210    2881

  Fragments arranged by size: 

                2881    1737     401     260     210     102      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    175     435    1710    2236    3868    4180    4326    5472     175 
   Size:        260    1275     526    1632     312     146    1146     365

  Fragments arranged by size: 

                1632    1275    1146     526     365     312     260     146
            
 
EcoICRI GAG'CTC

Cuts at:    792    2672     792 
   Size:       1880    3782
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    458     551     629     707     808    1061    1079    1159    1235 
   Size:         93      78      78     101     253      18      80      76

Cuts at:   1235    1283    1970    2462    2476    2516    2527    2606    3485 
   Size:         48     687     492      14      40      11      79     879

Cuts at:   3485    3917    4666    4934     458 
   Size:        432     749     268    1186

  Fragments arranged by size: 

                1186     879     749     687     492     432     268     253
                 101      93      80      79      78      78      76      48
                  40      18      14      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1312    2203    2621    1312 
   Size:        891     418    4353

  Fragments arranged by size: 

                4353     891     418
 
EcoO109I rG'GnC_Cy

Cuts at:    229     704     825     834    1008    1630    1884    1998    2353 
   Size:        475     121       9     174     622     254     114     355

Cuts at:   2353    2522    2799     229 
   Size:        169     277    3092

  Fragments arranged by size: 

                3092     622     475     355     277     254     174     169
                 121     114       9
 
EcoRI G'AATT_C

Cuts at:   2032    2664    2032 
   Size:        632    5030
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3679    3679 
   Size:       5662
 
FspI TGC'GCA

Cuts at:   1816    3441    1816 
   Size:       1625    4037
 
HaeII r_GCGC'y

Cuts at:    444    1925    2028    2328    3094    3342    4366    5345     444 
   Size:       1481     103     300     766     248    1024     979     761

  Fragments arranged by size: 

                1481    1024     979     766     761     300     248     103
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   3502    3532    3534    3564    3502 
   Size:         30       2      30    5600

  Fragments arranged by size: 

                5600      30      30       2
 
HincII GTy'rAC

Cuts at:   2699    2699 
   Size:       5662
 
HindIII A'AGCT_T

Cuts at:   2300    2300 
   Size:       5662
 
KasI G'GCGC_C

Cuts at:   2024    3090    3338    2024 
   Size:       1066     248    4348

  Fragments arranged by size: 

                4348    1066     248
 
KpnI G_GTAC'C

Cuts at:   2680    2680 
   Size:       5662
 
MluI A'CGCG_T

Cuts at:   5095    5095 
   Size:       5662
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2592    4333    4517    2592 
   Size:       1741     184    3737

  Fragments arranged by size: 

                3737    1741     184
 
MscI TGG'CCA

Cuts at:   3421    3421 
   Size:       5662
 
MslI CAynn'nnrTG

Cuts at:   1453    1708    3649    3776    1453 
   Size:        255    1941     127    3339

  Fragments arranged by size: 

                3339    1941     255     127
 
MspA1I CmG'CkG

Cuts at:    325     855    1340    1770    2166    2538    2771    3085    3445 
   Size:        530     485     430     396     372     233     314     360

Cuts at:   3445    4460    4705     325 
   Size:       1015     245    1282

  Fragments arranged by size: 

                1282    1015     530     485     430     396     372     360
                 314     245     233
 
NaeI GCC'GGC

Cuts at:   1735    3841    1735 
   Size:       2106    3556
 
NarI GG'CG_CC

Cuts at:   2025    3091    3339    2025 
   Size:       1066     248    4348

  Fragments arranged by size: 

                4348    1066     248
 
NcoI C'CATG_G

Cuts at:    524     524 
   Size:       5662
 
NgoMIV G'CCGG_C

Cuts at:   1733    3839    1733 
   Size:       2106    3556
 
NruI TCG'CGA

Cuts at:   4023    4023 
   Size:       5662
 
NspI r_CATG'y

Cuts at:   1380    1618    1821    2322    2713    3213    4122    1380 
   Size:        238     203     501     391     500     909    2920

  Fragments arranged by size: 

                2920     909     501     500     391     238     203
 
PasI CC'CwG_GG

Cuts at:    226     830    1887     226 
   Size:        604    1057    4001

  Fragments arranged by size: 

                4001    1057     604
 
PciI A'CATG_T

Cuts at:   4118    4118 
   Size:       5662
 
PflMI CCAn_nnn'nTGG

Cuts at:    311     730    1626     311 
   Size:        419     896    4347

  Fragments arranged by size: 

                4347     896     419
 
PfoI T'CCnGG_A

Cuts at:   1472    1472 
   Size:       5662
 
PmeI GTTT'AAAC

Cuts at:   2659    2659 
   Size:       5662
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4801    4833    4801 
   Size:         32    5630
 
PpuMI rG'GwC_Cy

Cuts at:    704     825     834    1008     704 
   Size:        121       9     174    5358

  Fragments arranged by size: 

                5358     174     121       9
 
PstI C_TGCA'G

Cuts at:    150    1169    1742    1829    2707    3392     150 
   Size:       1019     573      87     878     685    2420

  Fragments arranged by size: 

                2420    1019     878     685     573      87
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5662
 
PvuII CAG'CTG

Cuts at:    325    2538    3085    3445     325 
   Size:       2213     547     360    2542

  Fragments arranged by size: 

                2542    2213     547     360
 
RsrII CG'GwC_CG

Cuts at:   3855    3855 
   Size:       5662
 
SacI G_AGCT'C

Cuts at:    794    2674     794 
   Size:       1880    3782
 
SalI G'TCGA_C

Cuts at:   2697    2697 
   Size:       5662
 
SanDI GG'GwC_CC

Cuts at:    704     834    1008     704 
   Size:        130     174    5358

  Fragments arranged by size: 

                5358     174     130
 
SapI GCTCTTCn'nnn_

Cuts at:   1584    1946    3683    3893    1584 
   Size:        362    1737     210    3353

  Fragments arranged by size: 

                3353    1737     362     210
 
SbfI CC_TGCA'GG

Cuts at:   1169    2707    1169 
   Size:       1538    4124
 
ScaI AGT'ACT

Cuts at:   5179    5179 
   Size:       5662
 
SfcI C'TryA_G

Cuts at:     33     146    1165    1686    1738    1825    2703    3388    4383 
   Size:        113    1019     521      52      87     878     685     995

Cuts at:   4383    4574      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121    1019     995     878     685     521     191     113
                  87      52
 
SfoI GGC'GCC

Cuts at:   2026    3092    3340    2026 
   Size:       1066     248    4348

  Fragments arranged by size: 

                4348    1066     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5662
 
SmaI CCC'GGG

Cuts at:   2682    2682 
   Size:       5662
 
SmlI C'TyrA_G

Cuts at:    334     958    2602    2815    3730    4224    4486     334 
   Size:        624    1644     213     915     494     262    1510

  Fragments arranged by size: 

                1644    1510     915     624     494     262     213
 
SnaBI TAC'GTA

Cuts at:   2652    2652 
   Size:       5662
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5662
 
SphI G_CATG'C

Cuts at:   1380    1618    1821    2713    3213    1380 
   Size:        238     203     892     500    3829

  Fragments arranged by size: 

                3829     892     500     238     203
 
StyI C'CwwG_G

Cuts at:    524    2794    3148     524 
   Size:       2270     354    3038

  Fragments arranged by size: 

                3038    2270     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    501     789     883    1219    1287    1459    1879    1984    2299 
   Size:        288      94     336      68     172     420     105     315

Cuts at:   2299    3243    3376    3835    4046     501 
   Size:        944     133     459     211    2117

  Fragments arranged by size: 

                2117     944     459     420     336     315     288     211
                 172     133     105      94      68
 
TatI w'GTAC_w

Cuts at:   5177    5177 
   Size:       5662
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    229     261     411     443    1262    1294    5179    5211     229 
   Size:         32     150      32     819      32    3885      32     680

  Fragments arranged by size: 

                3885     819     680     150      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2450    3457    2450 
   Size:       1007    4655
 
XbaI T'CTAG_A

Cuts at:    367    2691     367 
   Size:       2324    3338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1344    3155    1344 
   Size:       1811    3851
 
XhoI C'TCGA_G

Cuts at:   2602    2602 
   Size:       5662
 
XmaI C'CCGG_G

Cuts at:   2680    2680 
   Size:       5662
 
ZraI GAC'GTC

Cuts at:   1406    1406 
   Size:       5662
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NruI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AloI     ApaI     AscI    AvrII    BfrBI    BmgBI
     BmtI    BsiWI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     NsiI     PacI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI     SrfI     SspI
     StuI     SwaI     XmnI
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