Restriction map for
pF1KSDA0920
(Circular) (Six-base) MAPSORT of: pF1KSDA0920.seq Check: 1860 from: 1 to: 6448
pF1KSDA0920 6448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1251 2562 1251
Size: 1311 5137
AccI GT'mk_AC
Cuts at: 3484 3484
Size: 6448
Acc65I G'GTAC_C
Cuts at: 3462 3462
Size: 6448
AclI AA'CG_TT
Cuts at: 6288 6288
Size: 6448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 481 1436 2648 2669 2777 3046 3182 3215
Size: 125 955 1212 21 108 269 136 33
Cuts at: 3215 4271 4703 5452 5720 356
Size: 1056 432 749 268 1084
Fragments arranged by size:
1212 1084 1056 955 749 432 269 268
136 125 108 33 21
AflIII A'CryG_T
Cuts at: 3798 4904 5881 3798
Size: 1106 977 4365
Fragments arranged by size:
4365 1106 977
AgeI A'CCGG_T
Cuts at: 4836 4836
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 2250 2250
Size: 6448
AlwNI CAG_nnn'CTG
Cuts at: 384 1060 1653 1714 2796 3751 5320 384
Size: 676 593 61 1082 955 1569 1512
Fragments arranged by size:
1569 1512 1082 955 676 593 61
ApaI G_GGCC'C
Cuts at: 2069 2096 2786 2069
Size: 27 690 5731
Fragments arranged by size:
5731 690 27
ApaLI G'TGCA_C
Cuts at: 5218 5218
Size: 6448
ApoI r'AATT_y
Cuts at: 1821 2125 2364 3450 5801 6057 1821
Size: 304 239 1086 2351 256 2212
Fragments arranged by size:
2351 2212 1086 304 256 239
AseI AT'TA_AT
Cuts at: 20 20
Size: 6448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6448
AvaI C'yCGr_G
Cuts at: 1307 2138 3343 3466 1307
Size: 831 1205 123 4289
Fragments arranged by size:
4289 1205 831 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2261 2294 2261
Size: 33 6415
BamHI G'GATC_C
Cuts at: 3471 3471
Size: 6448
BanI G'GyrC_C
Cuts at: 1149 1263 1969 2002 3462 3876 4124 4159 1149
Size: 114 706 33 1460 414 248 35 3438
Fragments arranged by size:
3438 1460 706 414 248 114 35 33
BanII G_rGCy'C
Cuts at: 1169 1493 2069 2096 2786 3064 3460 4490 1169
Size: 324 576 27 690 278 396 1030 3127
Fragments arranged by size:
3127 1030 690 576 396 324 278 27
BbeI G_GCGC'C
Cuts at: 3880 4128 3880
Size: 248 6200
BbsI GAAGACnn'nnnn_
Cuts at: 1967 2699 2765 3254 3431 1967
Size: 732 66 489 177 4984
Fragments arranged by size:
4984 732 489 177 66
BbvCI CC'TCA_GC
Cuts at: 1974 2114 2950 1974
Size: 140 836 5472
Fragments arranged by size:
5472 836 140
BciVI GTATCCnnnnn_n'
Cuts at: 2262 4344 5107 6448 2262
Size: 2082 763 1341 2262
Fragments arranged by size:
2262 2082 1341 763
BclI T'GATC_A
Cuts at: 936 2454 3966 936
Size: 1518 1512 3418
Fragments arranged by size:
3418 1518 1512
BglII A'GATC_T
Cuts at: 2197 3961 6000 2197
Size: 1764 2039 2645
Fragments arranged by size:
2645 2039 1764
BlpI GC'TnA_GC
Cuts at: 2882 3558 2882
Size: 676 5772
Bme1580I G_kGCm'C
Cuts at: 1638 2007 2069 2096 2786 4071 4164 5222 1638
Size: 369 62 27 690 1285 93 1058 2864
Fragments arranged by size:
2864 1285 1058 690 369 93 62 27
BmgBI CAC'GTC
Cuts at: 2928 2928
Size: 6448
BmrI ACTGGGnnnn_n'
Cuts at: 2807 3658 3739 4074 6220 2807
Size: 851 81 335 2146 3035
Fragments arranged by size:
3035 2146 851 335 81
BmtI G_CTAG'C
Cuts at: 1142 1142
Size: 6448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 681 2250 4813 681
Size: 1569 2563 2316
Fragments arranged by size:
2563 2316 1569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 489 641 799 1486 1615 1940 2029 2302
Size: 83 152 158 687 129 325 89 273
Cuts at: 2302 2341 3078 3101 406
Size: 39 737 23 3753
Fragments arranged by size:
3753 737 687 325 273 158 152 129
89 83 39 23
Bpu10I CC'TnA_GC
Cuts at: 1087 1974 2114 2770 2950 3005 1087
Size: 887 140 656 180 55 4530
Fragments arranged by size:
4530 887 656 180 140 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2178 2900 3622 4501 4995 5293 2178
Size: 722 722 879 494 298 3333
Fragments arranged by size:
3333 879 722 722 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1926 2005 2267 2278 2830 6152 1926
Size: 79 262 11 552 3322 2222
Fragments arranged by size:
3322 2222 552 262 79 11
BsaAI yAC'GTr
Cuts at: 3438 3799 3438
Size: 361 6087
BsaBI GATnn'nnATC
Cuts at: 6 3960 5999 6
Size: 3954 2039 455
Fragments arranged by size:
3954 2039 455
BsaHI Gr'CG_yC
Cuts at: 2435 3877 4125 4829 6325 2435
Size: 1442 248 704 1496 2558
Fragments arranged by size:
2558 1496 1442 704 248
BsaWI w'CCGG_w
Cuts at: 3635 4156 4836 5110 5257 5668 3635
Size: 521 680 274 147 411 4415
Fragments arranged by size:
4415 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 523 539 553 880 910 1336 1366 2420
Size: 14 16 14 327 30 426 30 1054
Cuts at: 2420 2450 509
Size: 30 4507
Fragments arranged by size:
4507 1054 426 327 30 30 30 16
14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 1145 1471 1497 1585 2239 2441 2848 3034
Size: 820 326 26 88 654 202 407 186
Cuts at: 3034 3134 325
Size: 100 3639
Fragments arranged by size:
3639 820 654 407 326 202 186 100
88 26
BseYI C'CCAG_C
Cuts at: 542 716 1112 1225 1245 1276 2484 2789 3169
Size: 174 396 113 20 31 1208 305 380
Cuts at: 3169 3212 3389 3871 4629 5208 5704 542
Size: 43 177 482 758 579 496 1286
Fragments arranged by size:
1286 1208 758 579 496 482 396 380
305 177 174 113 43 31 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1689 2560 2827 1085
Size: 604 871 267 4706
Fragments arranged by size:
4706 871 604 267
BsiEI CG_ry'CG
Cuts at: 104 3988 4034 5244 104
Size: 3884 46 1210 1308
Fragments arranged by size:
3884 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1169 1493 3460 4238 4428 5222 1169
Size: 324 1967 778 190 794 2395
Fragments arranged by size:
2395 1967 794 778 324 190
BsmI GAATG_Cn'
Cuts at: 243 2550 3081 243
Size: 2307 531 3610
Fragments arranged by size:
3610 2307 531
BsmBI CGTCTCn'nnnn_
Cuts at: 2442 2442
Size: 6448
Bsp1286I G_dGCh'C
Cuts at: 1169 1493 1638 2007 2069 2096 2786 3064 3460
Size: 324 145 369 62 27 690 278 396
Cuts at: 3460 4071 4164 4238 4428 4490 5222 1169
Size: 611 93 74 190 62 732 2395
Fragments arranged by size:
2395 732 690 611 396 369 324 278
190 145 93 74 62 62 27
BspEI T'CCGG_A
Cuts at: 5668 5668
Size: 6448
BspHI T'CATG_A
Cuts at: 1725 5624 1725
Size: 3899 2549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 563 1251 2562 3496 3710 4012 4393 563
Size: 688 1311 934 214 302 381 2618
Fragments arranged by size:
2618 1311 934 688 381 302 214
BsrBI CCG'CTC
Cuts at: 50 616 4738 4792 6278 6443 50
Size: 566 4122 54 1486 165 55
Fragments arranged by size:
4122 1486 566 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1526 4358 1526
Size: 2832 3616
BsrFI r'CCGG_y
Cuts at: 1142 4444 4625 4836 1142
Size: 3302 181 211 2754
Fragments arranged by size:
3302 2754 211 181
BssSI C'ACGA_G
Cuts at: 1102 4717 5077 1102
Size: 3615 360 2473
Fragments arranged by size:
3615 2473 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3823 3823
Size: 6448
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6448
BstXI CCAn_nnnn'nTGG
Cuts at: 777 2280 777
Size: 1503 4945
BstYI r'GATC_y
Cuts at: 1 974 2197 2993 3401 3471 3938 3961 4296
Size: 973 1223 796 408 70 467 23 335
Cuts at: 4296 4542 5556 5642 5654 6000 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1223 1014 973 796 467 449 408 346
335 246 86 70 23 12
Bsu36I CC'TnA_GG
Cuts at: 2699 2699
Size: 6448
BtgI C'CryG_G
Cuts at: 125 884 1190 2172 125
Size: 759 306 982 4401
Fragments arranged by size:
4401 982 759 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1679 4396 4577 4740 1679
Size: 2717 181 163 3387
Fragments arranged by size:
3387 2717 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 2794 3085 3689 1073
Size: 1721 291 604 3832
Fragments arranged by size:
3832 1721 604 291
DraI TTT'AAA
Cuts at: 3445 3445
Size: 6448
DrdI GACnn_nn'nnGTC
Cuts at: 4152 5012 4152
Size: 860 5588
EaeI y'GGCC_r
Cuts at: 1215 1838 2909 4031 4205 4596 4623 5970 1215
Size: 623 1071 1122 174 391 27 1347 1693
Fragments arranged by size:
1693 1347 1122 1071 623 391 174 27
EagI C'GGCC_G
Cuts at: 4031 4031
Size: 6448
EarI CTCTTCn'nnn_
Cuts at: 302 332 410 1725 2984 3011 3022 3191 4469
Size: 30 78 1315 1259 27 11 169 1278
Cuts at: 4469 4679 302
Size: 210 2071
Fragments arranged by size:
2071 1315 1278 1259 210 169 78 30
27 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 4654 4966 5112 6258 151
Size: 4503 312 146 1146 341
Fragments arranged by size:
4503 1146 341 312 146
EcoICRI GAG'CTC
Cuts at: 1167 1491 3458 1167
Size: 324 1967 4157
Fragments arranged by size:
4157 1967 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 406 481 489 641 799 1436 1486 1615
Size: 50 75 8 152 158 637 50 129
Cuts at: 1615 1940 2029 2302 2341 2648 2669 2777 3046
Size: 325 89 273 39 307 21 108 269
Cuts at: 3046 3078 3101 3182 3215 4271 4703 5452 5720
Size: 32 23 81 33 1056 432 749 268
Cuts at: 5720 356
Size: 1084
Fragments arranged by size:
1084 1056 749 637 432 325 307 273
269 268 158 152 129 108 89 81
75 50 50 39 33 32 23 21
8
EcoO109I rG'GnC_Cy
Cuts at: 1951 2065 2066 2093 2623 2782 2783 3166 3339
Size: 114 1 27 530 159 1 383 173
Cuts at: 3339 3585 1951
Size: 246 4814
Fragments arranged by size:
4814 530 383 246 173 159 114 27
1 1
EcoRI G'AATT_C
Cuts at: 1821 3450 1821
Size: 1629 4819
EcoRV GAT'ATC
Cuts at: 428 735 428
Size: 307 6141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 4465 2245
Size: 2220 4228
FspI TGC'GCA
Cuts at: 4227 4227
Size: 6448
HaeII r_GCGC'y
Cuts at: 2138 2770 3880 4128 5152 6131 2138
Size: 632 1110 248 1024 979 2455
Fragments arranged by size:
2455 1110 1024 979 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 880 912 966 998 1478 1510 2002 2034 2169
Size: 32 54 32 480 32 492 32 135
Cuts at: 2169 2176 2201 2208 2236 2268 2275 2307 2398
Size: 7 25 7 28 32 7 32 91
Cuts at: 2398 2420 2430 2452 4288 4318 4320 4350 880
Size: 22 10 22 1836 30 2 30 2978
Fragments arranged by size:
2978 1836 492 480 135 91 54 32
32 32 32 32 32 30 30 28
25 22 22 10 7 7 7 2
HincII GTy'rAC
Cuts at: 1905 3485 1905
Size: 1580 4868
KasI G'GCGC_C
Cuts at: 3876 4124 3876
Size: 248 6200
KpnI G_GTAC'C
Cuts at: 3466 3466
Size: 6448
MluI A'CGCG_T
Cuts at: 5881 5881
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5119 5303 5119
Size: 184 6264
MscI TGG'CCA
Cuts at: 1217 1840 2911 4207 1217
Size: 623 1071 1296 3458
Fragments arranged by size:
3458 1296 1071 623
MslI CAynn'nnrTG
Cuts at: 4435 4562 4435
Size: 127 6321
MspA1I CmG'CkG
Cuts at: 295 1578 1812 2327 2803 2881 2967 3216 3557
Size: 1283 234 515 476 78 86 249 341
Cuts at: 3557 3871 4231 5246 5491 295
Size: 314 360 1015 245 1252
Fragments arranged by size:
1283 1252 1015 515 476 360 341 314
249 245 234 86 78
NaeI GCC'GGC
Cuts at: 1144 4627 1144
Size: 3483 2965
NarI GG'CG_CC
Cuts at: 3877 4125 3877
Size: 248 6200
NcoI C'CATG_G
Cuts at: 125 884 1190 2172 125
Size: 759 306 982 4401
Fragments arranged by size:
4401 982 759 306
NgoMIV G'CCGG_C
Cuts at: 1142 4625 1142
Size: 3483 2965
NheI G'CTAG_C
Cuts at: 1138 1138
Size: 6448
NruI TCG'CGA
Cuts at: 4809 4809
Size: 6448
NspI r_CATG'y
Cuts at: 2688 3104 3499 3999 4908 2688
Size: 416 395 500 909 4228
Fragments arranged by size:
4228 909 500 416 395
PciI A'CATG_T
Cuts at: 4904 4904
Size: 6448
PflMI CCAn_nnn'nTGG
Cuts at: 592 1060 592
Size: 468 5980
PfoI T'CCnGG_A
Cuts at: 776 858 2279 2357 2956 776
Size: 82 1421 78 599 4268
Fragments arranged by size:
4268 1421 599 82 78
PmeI GTTT'AAAC
Cuts at: 3445 3445
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 331 363 5587 5619 331
Size: 32 5224 32 1160
Fragments arranged by size:
5224 1160 32 32
PpuMI rG'GwC_Cy
Cuts at: 1951 2623 3339 1951
Size: 672 716 5060
Fragments arranged by size:
5060 716 672
PshAI GACnn'nnGTC
Cuts at: 2237 2237
Size: 6448
PspOMI G'GGCC_C
Cuts at: 2065 2092 2782 2065
Size: 27 690 5731
Fragments arranged by size:
5731 690 27
PspXI vC'TCGA_Gb
Cuts at: 3343 3343
Size: 6448
PstI C_TGCA'G
Cuts at: 242 284 1262 1661 3179 3493 4178 242
Size: 42 978 399 1518 314 685 2512
Fragments arranged by size:
2512 1518 978 685 399 314 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6448
PvuII CAG'CTG
Cuts at: 1578 1812 2803 2881 3871 4231 1578
Size: 234 991 78 990 360 3795
Fragments arranged by size:
3795 991 990 360 234 78
RsrII CG'GwC_CG
Cuts at: 4641 4641
Size: 6448
SacI G_AGCT'C
Cuts at: 1169 1493 3460 1169
Size: 324 1967 4157
Fragments arranged by size:
4157 1967 324
SalI G'TCGA_C
Cuts at: 3483 3483
Size: 6448
SapI GCTCTTCn'nnn_
Cuts at: 4469 4679 4469
Size: 210 6238
SbfI CC_TGCA'GG
Cuts at: 1262 3493 1262
Size: 2231 4217
ScaI AGT'ACT
Cuts at: 2869 5965 2869
Size: 3096 3352
SfcI C'TryA_G
Cuts at: 33 238 280 762 1258 1657 1884 2070 2636
Size: 205 42 482 496 399 227 186 566
Cuts at: 2636 3175 3489 4174 5169 5360 33
Size: 539 314 685 995 191 1121
Fragments arranged by size:
1121 995 685 566 539 496 482 399
314 227 205 191 186 42
SfoI GGC'GCC
Cuts at: 3878 4126 3878
Size: 248 6200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6448
SmaI CCC'GGG
Cuts at: 2140 3468 2140
Size: 1328 5120
SmlI C'TyrA_G
Cuts at: 2193 2915 3343 3601 4516 5010 5272 2193
Size: 722 428 258 915 494 262 3369
Fragments arranged by size:
3369 915 722 494 428 262 258
SnaBI TAC'GTA
Cuts at: 3438 3438
Size: 6448
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6448
SphI G_CATG'C
Cuts at: 3499 3999 3499
Size: 500 5948
SrfI GCCC'GGGC
Cuts at: 2140 2140
Size: 6448
StuI AGG'CCT
Cuts at: 1449 1890 1449
Size: 441 6007
StyI C'CwwG_G
Cuts at: 125 884 1190 1841 1982 2172 2513 3580 3934
Size: 759 306 651 141 190 341 1067 354
Cuts at: 3934 125
Size: 2639
Fragments arranged by size:
2639 1067 759 651 354 341 306 190
141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1872 4029 4162 4621 4832 1872
Size: 2157 133 459 211 3488
Fragments arranged by size:
3488 2157 459 211 133
TatI w'GTAC_w
Cuts at: 1610 1852 2867 5963 1610
Size: 242 1015 3096 2095
Fragments arranged by size:
3096 2095 1015 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 931 963 5965 5997 931
Size: 32 5002 32 1382
Fragments arranged by size:
5002 1382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 2973 4243 1765
Size: 1208 1270 3970
Fragments arranged by size:
3970 1270 1208
XbaI T'CTAG_A
Cuts at: 984 3477 984
Size: 2493 3955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1833 3941 1833
Size: 2108 4340
XhoI C'TCGA_G
Cuts at: 3343 3343
Size: 6448
XmaI C'CCGG_G
Cuts at: 2138 3466 2138
Size: 1328 5120
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 6448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BcgI BfrBI BglI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NotI NsiI PacI PasI PmlI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SspI SwaI
ZraI