Restriction map for
pF1KSDA0936
(Circular) (Six-base) MAPSORT of: pF1KSDA0936.seq Check: 2387 from: 1 to: 5033
pF1KSDA0936 5033 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1098 1098
Size: 5033
AatII G_ACGT'C
Cuts at: 674 1540 674
Size: 866 4167
AccI GT'mk_AC
Cuts at: 646 2071 646
Size: 1425 3608
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 4873 4873
Size: 5033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 904 1000 1510 1694 1903 2858 3290 4037
Size: 83 96 510 184 209 955 432 747
Cuts at: 4037 4305 821
Size: 268 1549
Fragments arranged by size:
1549 955 747 510 432 268 209 184
96 83
AflIII A'CryG_T
Cuts at: 2385 3489 4466 2385
Size: 1104 977 2952
Fragments arranged by size:
2952 1104 977
AgeI A'CCGG_T
Cuts at: 3421 3421
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 906 2338 3905 906
Size: 1432 1567 2034
Fragments arranged by size:
2034 1567 1432
ApaLI G'TGCA_C
Cuts at: 3803 3803
Size: 5033
ApoI r'AATT_y
Cuts at: 238 758 1121 1660 1740 2037 4386 4642 238
Size: 520 363 539 80 297 2349 256 629
Fragments arranged by size:
2349 629 539 520 363 297 256 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 5033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 154 566 2053 154
Size: 412 1487 3134
Fragments arranged by size:
3134 1487 412
BamHI G'GATC_C
Cuts at: 927 2058 927
Size: 1131 3902
BanI G'GyrC_C
Cuts at: 2049 2463 2711 2746 2049
Size: 414 248 35 4336
Fragments arranged by size:
4336 414 248 35
BanII G_rGCy'C
Cuts at: 627 2047 3077 627
Size: 1420 1030 2583
Fragments arranged by size:
2583 1420 1030
BbeI G_GCGC'C
Cuts at: 2467 2715 2467
Size: 248 4785
BbvCI CC'TCA_GC
Cuts at: 1414 1414
Size: 5033
BciVI GTATCCnnnnn_n'
Cuts at: 2931 3692 5033 2931
Size: 761 1341 2931
Fragments arranged by size:
2931 1341 761
BclI T'GATC_A
Cuts at: 342 2553 342
Size: 2211 2822
BfrBI ATG'CAT
Cuts at: 260 260
Size: 5033
BglII A'GATC_T
Cuts at: 1570 2548 4585 1570
Size: 978 2037 2018
Fragments arranged by size:
2037 2018 978
BlpI GC'TnA_GC
Cuts at: 2145 2145
Size: 5033
Bme1580I G_kGCm'C
Cuts at: 1513 1527 2658 2751 3807 1513
Size: 14 1131 93 1056 2739
Fragments arranged by size:
2739 1131 1056 93 14
BmrI ACTGGGnnnn_n'
Cuts at: 2004 2245 2326 2661 4805 2004
Size: 241 81 335 2144 2232
Fragments arranged by size:
2232 2144 335 241 81
BmtI G_CTAG'C
Cuts at: 887 887
Size: 5033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 749 1176 1201 1315 1946 611
Size: 138 427 25 114 631 3698
Fragments arranged by size:
3698 631 427 138 114 25
Bpu10I CC'TnA_GC
Cuts at: 1414 1414
Size: 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 3088 3580 3878 2209
Size: 879 492 298 3364
Fragments arranged by size:
3364 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1194 1887 4737 1194
Size: 693 2850 1490
Fragments arranged by size:
2850 1490 693
BsaAI yAC'GTr
Cuts at: 1169 2025 2386 1169
Size: 856 361 3816
Fragments arranged by size:
3816 856 361
BsaBI GATnn'nnATC
Cuts at: 6 2547 4584 6
Size: 2541 2037 455
Fragments arranged by size:
2541 2037 455
BsaHI Gr'CG_yC
Cuts at: 671 1537 2464 2712 3414 4910 671
Size: 866 927 248 702 1496 794
Fragments arranged by size:
1496 927 866 794 702 248
BsaWI w'CCGG_w
Cuts at: 2222 2743 3421 3695 3842 4253 2222
Size: 521 678 274 147 411 3002
Fragments arranged by size:
3002 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 643 673 265
Size: 30 348 30 4625
Fragments arranged by size:
4625 348 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 508
Size: 5033
BseYI C'CCAG_C
Cuts at: 183 771 1094 1925 1970 2458 3216 3793 4289
Size: 588 323 831 45 488 758 577 496
Cuts at: 4289 183
Size: 927
Fragments arranged by size:
927 831 758 588 577 496 488 323
45
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3829 104
Size: 2471 46 1208 1308
Fragments arranged by size:
2471 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1301 2047 2825 3015 3807 1301
Size: 746 778 190 792 2527
Fragments arranged by size:
2527 792 778 746 190
BsmI GAATG_Cn'
Cuts at: 262 262
Size: 5033
Bsp1286I G_dGCh'C
Cuts at: 627 1301 1513 1527 2047 2658 2751 2825 3015
Size: 674 212 14 520 611 93 74 190
Cuts at: 3015 3077 3807 627
Size: 62 730 1853
Fragments arranged by size:
1853 730 674 611 520 212 190 93
74 62 14
BspEI T'CCGG_A
Cuts at: 4253 4253
Size: 5033
BspHI T'CATG_A
Cuts at: 4209 4209
Size: 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1098 1457 2083 2297 2599 2980 1098
Size: 359 626 214 302 381 3151
Fragments arranged by size:
3151 626 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 3325 3379 4863 5028 50
Size: 3275 54 1484 165 55
Fragments arranged by size:
3275 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 830 2945 830
Size: 2115 2918
BsrFI r'CCGG_y
Cuts at: 3031 3212 3421 3031
Size: 181 209 4643
Fragments arranged by size:
4643 209 181
BssSI C'ACGA_G
Cuts at: 3304 3662 3304
Size: 358 4675
BstAPI GCAn_nnn'nTGC
Cuts at: 2410 2410
Size: 5033
BstBI TT'CG_AA
Cuts at: 110 3394 110
Size: 3284 1749
BstYI r'GATC_y
Cuts at: 1 927 1570 2058 2525 2548 2883 3129 4141
Size: 926 643 488 467 23 335 246 1012
Cuts at: 4141 4227 4239 4585 1
Size: 86 12 346 449
Fragments arranged by size:
1012 926 643 488 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 640 712 640
Size: 72 4961
BtgI C'CryG_G
Cuts at: 1678 1954 1678
Size: 276 4757
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2983 3164 3327 2983
Size: 181 163 4689
Fragments arranged by size:
4689 181 163
BtsI GCAGTG_nn'
Cuts at: 894 2276 894
Size: 1382 3651
DraI TTT'AAA
Cuts at: 287 2032 287
Size: 1745 3288
DraIII CAC_nnn'GTG
Cuts at: 847 847
Size: 5033
DrdI GACnn_nn'nnGTC
Cuts at: 2739 3597 2739
Size: 858 4175
EaeI y'GGCC_r
Cuts at: 838 1983 2618 2792 3183 3210 4555 838
Size: 1145 635 174 391 27 1345 1316
Fragments arranged by size:
1345 1316 1145 635 391 174 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 727 3056 3266 727
Size: 2329 210 2494
Fragments arranged by size:
2494 2329 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3241 3551 3697 4843 3241
Size: 310 146 1146 3431
Fragments arranged by size:
3431 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2045 2045
Size: 5033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 749 821 904 1000 1176 1201 1315 1510
Size: 138 72 83 96 176 25 114 195
Cuts at: 1510 1694 1903 1946 2858 3290 4037 4305 611
Size: 184 209 43 912 432 747 268 1339
Fragments arranged by size:
1339 912 747 432 268 209 195 184
176 138 114 96 83 72 43 25
EcoNI CCTnn'n_nnAGG
Cuts at: 271 1051 271
Size: 780 4253
EcoO109I rG'GnC_Cy
Cuts at: 2172 2172
Size: 5033
EcoRI G'AATT_C
Cuts at: 2037 2037
Size: 5033
EcoRV GAT'ATC
Cuts at: 967 967
Size: 5033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 5033
FspI TGC'GCA
Cuts at: 2814 2814
Size: 5033
HaeII r_GCGC'y
Cuts at: 2467 2715 3737 4716 2467
Size: 248 1022 979 2784
Fragments arranged by size:
2784 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 589 621 1527 1559 1884 1916 2875
Size: 32 292 32 906 32 325 32 959
Cuts at: 2875 2905 2907 2937 265
Size: 30 2 30 2361
Fragments arranged by size:
2361 959 906 325 292 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1358 2072 1358
Size: 714 4319
KasI G'GCGC_C
Cuts at: 2463 2711 2463
Size: 248 4785
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 963 3704 3888 963
Size: 2741 184 2108
Fragments arranged by size:
2741 2108 184
MscI TGG'CCA
Cuts at: 840 2794 840
Size: 1954 3079
MslI CAynn'nnrTG
Cuts at: 305 1304 3022 3149 305
Size: 999 1718 127 2189
Fragments arranged by size:
2189 1718 999 127
MspA1I CmG'CkG
Cuts at: 1288 1548 2144 2458 2818 3831 4076 1288
Size: 260 596 314 360 1013 245 2245
Fragments arranged by size:
2245 1013 596 360 314 260 245
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5033
NarI GG'CG_CC
Cuts at: 2464 2712 2464
Size: 248 4785
NcoI C'CATG_G
Cuts at: 1678 1678
Size: 5033
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5033
NheI G'CTAG_C
Cuts at: 883 883
Size: 5033
NsiI A_TGCA'T
Cuts at: 262 262
Size: 5033
NspI r_CATG'y
Cuts at: 918 2086 2586 3493 918
Size: 1168 500 907 2458
Fragments arranged by size:
2458 1168 907 500
PciI A'CATG_T
Cuts at: 3489 3489
Size: 5033
PflMI CCAn_nnn'nTGG
Cuts at: 771 1981 771
Size: 1210 3823
PfoI T'CCnGG_A
Cuts at: 1046 1192 1217 1046
Size: 146 25 4862
Fragments arranged by size:
4862 146 25
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 5001
PstI C_TGCA'G
Cuts at: 1468 1578 2080 2765 1468
Size: 110 502 685 3736
Fragments arranged by size:
3736 685 502 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 1288 2458 2818 1288
Size: 1170 360 3503
Fragments arranged by size:
3503 1170 360
RsrII CG'GwC_CG
Cuts at: 3228 3228
Size: 5033
SacI G_AGCT'C
Cuts at: 2047 2047
Size: 5033
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 3056 3266 3056
Size: 210 4823
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 635 4550 635
Size: 3915 1118
SfcI C'TryA_G
Cuts at: 33 654 1464 1574 2076 2761 3754 3945 33
Size: 621 810 110 502 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 621 502 191 110
SfoI GGC'GCC
Cuts at: 2465 2713 2465
Size: 248 4785
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 2055 2055
Size: 5033
SmlI C'TyrA_G
Cuts at: 2188 3103 3595 3857 2188
Size: 915 492 262 3364
Fragments arranged by size:
3364 915 492 262
SnaBI TAC'GTA
Cuts at: 2025 2025
Size: 5033
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 2086 2586 2086
Size: 500 4533
SspI AAT'ATT
Cuts at: 752 752
Size: 5033
StyI C'CwwG_G
Cuts at: 1678 2167 2521 1678
Size: 489 354 4190
Fragments arranged by size:
4190 489 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1004 1220 1546 1939 2616 2749 3208 3417 1004
Size: 216 326 393 677 133 459 209 2620
Fragments arranged by size:
2620 677 459 393 326 216 209 133
TatI w'GTAC_w
Cuts at: 365 633 4548 365
Size: 268 3915 850
Fragments arranged by size:
3915 850 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4550 4582 4550
Size: 32 5001
Tth111I GACn'n_nGTC
Cuts at: 1719 2830 1719
Size: 1111 3922
XbaI T'CTAG_A
Cuts at: 2064 2064
Size: 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1978 2528 1676
Size: 302 550 4181
Fragments arranged by size:
4181 550 302
XmaI C'CCGG_G
Cuts at: 2053 2053
Size: 5033
XmnI GAAnn'nnTTC
Cuts at: 1125 1664 1707 1125
Size: 539 43 4451
Fragments arranged by size:
4451 539 43
ZraI GAC'GTC
Cuts at: 672 1538 672
Size: 866 4167
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BglI BmgBI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI