Restriction map for
pF1KSDA0940
(Circular) (Six-base) MAPSORT of: pF1KSDA0940.seq Check: 9213 from: 1 to: 5191
pF1KSDA0940 5191 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1490 2227 1490
Size: 737 4454
Acc65I G'GTAC_C
Cuts at: 2205 2205
Size: 5191
AclI AA'CG_TT
Cuts at: 5031 5031
Size: 5191
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 1912 3014 3446 4195 4463 1864
Size: 48 1102 432 749 268 2592
Fragments arranged by size:
2592 1102 749 432 268 48
AfeI AGC'GCT
Cuts at: 1911 1911
Size: 5191
AflII C'TTAA_G
Cuts at: 1353 1353
Size: 5191
AflIII A'CryG_T
Cuts at: 2541 3647 4624 2541
Size: 1106 977 3108
Fragments arranged by size:
3108 1106 977
AgeI A'CCGG_T
Cuts at: 3579 3579
Size: 5191
AlwNI CAG_nnn'CTG
Cuts at: 304 455 811 2494 4063 304
Size: 151 356 1683 1569 1432
Fragments arranged by size:
1683 1569 1432 356 151
ApaLI G'TGCA_C
Cuts at: 3961 3961
Size: 5191
ApoI r'AATT_y
Cuts at: 1236 1661 2193 4544 4800 1236
Size: 425 532 2351 256 1627
Fragments arranged by size:
2351 1627 532 425 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5191
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
AvaI C'yCGr_G
Cuts at: 2099 2209 2099
Size: 110 5081
BamHI G'GATC_C
Cuts at: 2214 2214
Size: 5191
BanI G'GyrC_C
Cuts at: 417 466 720 969 2205 2619 2867 2902 417
Size: 49 254 249 1236 414 248 35 2706
Fragments arranged by size:
2706 1236 414 254 249 248 49 35
BanII G_rGCy'C
Cuts at: 413 2203 3233 413
Size: 1790 1030 2371
Fragments arranged by size:
2371 1790 1030
BbeI G_GCGC'C
Cuts at: 724 2623 2871 724
Size: 1899 248 3044
Fragments arranged by size:
3044 1899 248
BbsI GAAGACnn'nnnn_
Cuts at: 555 785 555
Size: 230 4961
BbvCI CC'TCA_GC
Cuts at: 292 386 292
Size: 94 5097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1008 1042 1008
Size: 34 5157
BciVI GTATCCnnnnn_n'
Cuts at: 231 3087 3850 5191 231
Size: 2856 763 1341 231
Fragments arranged by size:
2856 1341 763 231
BclI T'GATC_A
Cuts at: 1203 1332 1784 1980 2709 1203
Size: 129 452 196 729 3685
Fragments arranged by size:
3685 729 452 196 129
BglI GCCn_nnn'nGGC
Cuts at: 313 748 835 313
Size: 435 87 4669
Fragments arranged by size:
4669 435 87
BglII A'GATC_T
Cuts at: 2704 4743 2704
Size: 2039 3152
BlpI GC'TnA_GC
Cuts at: 2301 2301
Size: 5191
Bme1580I G_kGCm'C
Cuts at: 469 2008 2814 2907 3965 469
Size: 1539 806 93 1058 1695
Fragments arranged by size:
1695 1539 1058 806 93
BmgBI CAC'GTC
Cuts at: 2160 2160
Size: 5191
BmrI ACTGGGnnnn_n'
Cuts at: 505 553 2401 2482 2817 4963 505
Size: 48 1848 81 335 2146 733
Fragments arranged by size:
2146 1848 733 335 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1584 3556 1584
Size: 1972 3219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 999 2193 900
Size: 99 1194 3898
Fragments arranged by size:
3898 1194 99
Bpu10I CC'TnA_GC
Cuts at: 292 386 292
Size: 94 5097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 2365 3244 3738 4036 387
Size: 1978 879 494 298 1542
Fragments arranged by size:
1978 1542 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 4895 247
Size: 4648 543
BsaAI yAC'GTr
Cuts at: 2181 2542 2181
Size: 361 4830
BsaBI GATnn'nnATC
Cuts at: 6 2703 4742 6
Size: 2697 2039 455
Fragments arranged by size:
2697 2039 455
BsaHI Gr'CG_yC
Cuts at: 721 2620 2868 3572 5068 721
Size: 1899 248 704 1496 844
Fragments arranged by size:
1899 1496 844 704 248
BsaWI w'CCGG_w
Cuts at: 1094 1133 2378 2899 3579 3853 4000 4411 1094
Size: 39 1245 521 680 274 147 411 1874
Fragments arranged by size:
1874 1245 680 521 411 274 147 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 237 374 750 237
Size: 137 376 4678
Fragments arranged by size:
4678 376 137
BseYI C'CCAG_C
Cuts at: 161 167 173 297 605 712 2081 2614 3372
Size: 6 6 124 308 107 1369 533 758
Cuts at: 3372 3951 4447 161
Size: 579 496 905
Fragments arranged by size:
1369 905 758 579 533 496 308 124
107 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 1942
Size: 5191
BsiEI CG_ry'CG
Cuts at: 104 941 2148 2731 2777 3987 104
Size: 837 1207 583 46 1210 1308
Fragments arranged by size:
1308 1210 1207 837 583 46
BsiHKAI G_wGCw'C
Cuts at: 2203 2981 3171 3965 2203
Size: 778 190 794 3429
Fragments arranged by size:
3429 794 778 190
BsiWI C'GTAC_G
Cuts at: 752 752
Size: 5191
BsmI GAATG_Cn'
Cuts at: 876 1873 876
Size: 997 4194
Bsp1286I G_dGCh'C
Cuts at: 413 469 2008 2203 2814 2907 2981 3171 3233
Size: 56 1539 195 611 93 74 190 62
Cuts at: 3233 3965 413
Size: 732 1639
Fragments arranged by size:
1639 1539 732 611 195 190 93 74
62 56
BspEI T'CCGG_A
Cuts at: 1094 4411 1094
Size: 3317 1874
BspHI T'CATG_A
Cuts at: 770 1206 4367 770
Size: 436 3161 1594
Fragments arranged by size:
3161 1594 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1460 2239 2453 2755 3136 1460
Size: 779 214 302 381 3515
Fragments arranged by size:
3515 779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 354 698 981 999 3481 3535 5021 5186
Size: 304 344 283 18 2482 54 1486 165
Cuts at: 5186 50
Size: 55
Fragments arranged by size:
2482 1486 344 304 283 165 55 54
18
BsrDI GCAATG_nn'
Cuts at: 1787 1895 3101 1787
Size: 108 1206 3877
Fragments arranged by size:
3877 1206 108
BsrFI r'CCGG_y
Cuts at: 285 648 3187 3368 3579 285
Size: 363 2539 181 211 1897
Fragments arranged by size:
2539 1897 363 211 181
BssSI C'ACGA_G
Cuts at: 3460 3820 3460
Size: 360 4831
BstAPI GCAn_nnn'nTGC
Cuts at: 811 2566 811
Size: 1755 3436
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5191
BstYI r'GATC_y
Cuts at: 1 2214 2681 2704 3039 3285 4299 4385 4397
Size: 2213 467 23 335 246 1014 86 12
Cuts at: 4397 4743 1
Size: 346 449
Fragments arranged by size:
2213 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 404 404
Size: 5191
BtgI C'CryG_G
Cuts at: 413 461 746 1326 1669 413
Size: 48 285 580 343 3935
Fragments arranged by size:
3935 580 343 285 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3139 3320 3483 3139
Size: 181 163 4847
Fragments arranged by size:
4847 181 163
BtsI GCAGTG_nn'
Cuts at: 287 1454 2432 287
Size: 1167 978 3046
Fragments arranged by size:
3046 1167 978
DraI TTT'AAA
Cuts at: 2188 2188
Size: 5191
DraIII CAC_nnn'GTG
Cuts at: 2014 2014
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 2895 3755 2895
Size: 860 4331
EaeI y'GGCC_r
Cuts at: 756 1863 2774 2948 3339 3366 4713 756
Size: 1107 911 174 391 27 1347 1234
Fragments arranged by size:
1347 1234 1107 911 391 174 27
EagI C'GGCC_G
Cuts at: 2774 2774
Size: 5191
EarI CTCTTCn'nnn_
Cuts at: 535 3212 3422 535
Size: 2677 210 2304
Fragments arranged by size:
2677 2304 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3397 3709 3855 5001 3397
Size: 312 146 1146 3587
Fragments arranged by size:
3587 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2201 2201
Size: 5191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 999 1864 1912 2193 3014 3446 4195 4463
Size: 99 865 48 281 821 432 749 268
Cuts at: 4463 900
Size: 1628
Fragments arranged by size:
1628 865 821 749 432 281 268 99
48
EcoNI CCTnn'n_nnAGG
Cuts at: 1217 1532 1217
Size: 315 4876
EcoO109I rG'GnC_Cy
Cuts at: 924 1142 2328 924
Size: 218 1186 3787
Fragments arranged by size:
3787 1186 218
EcoRI G'AATT_C
Cuts at: 2193 2193
Size: 5191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1535 3208 1535
Size: 1673 3518
FspI TGC'GCA
Cuts at: 2970 2970
Size: 5191
HaeII r_GCGC'y
Cuts at: 696 724 743 1913 2623 2871 3895 4874 696
Size: 28 19 1170 710 248 1024 979 1013
Fragments arranged by size:
1170 1024 1013 979 710 248 28 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 360 392 1274 1306 3031 3061 3063 3093 360
Size: 32 882 32 1725 30 2 30 2458
Fragments arranged by size:
2458 1725 882 32 32 30 30 2
HincII GTy'rAC
Cuts at: 516 555 2228 516
Size: 39 1673 3479
Fragments arranged by size:
3479 1673 39
HindIII A'AGCT_T
Cuts at: 1577 1577
Size: 5191
KasI G'GCGC_C
Cuts at: 720 2619 2867 720
Size: 1899 248 3044
Fragments arranged by size:
3044 1899 248
KpnI G_GTAC'C
Cuts at: 2209 2209
Size: 5191
MluI A'CGCG_T
Cuts at: 4624 4624
Size: 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 917 1151 1398 3862 4046 917
Size: 234 247 2464 184 2062
Fragments arranged by size:
2464 2062 247 234 184
MscI TGG'CCA
Cuts at: 2950 2950
Size: 5191
MslI CAynn'nnrTG
Cuts at: 3178 3305 3178
Size: 127 5064
MspA1I CmG'CkG
Cuts at: 186 304 415 748 811 848 2127 2171 2300
Size: 118 111 333 63 37 1279 44 129
Cuts at: 2300 2614 2974 3989 4234 186
Size: 314 360 1015 245 1143
Fragments arranged by size:
1279 1143 1015 360 333 314 245 129
118 111 63 44 37
NaeI GCC'GGC
Cuts at: 287 650 3370 287
Size: 363 2720 2108
Fragments arranged by size:
2720 2108 363
NarI GG'CG_CC
Cuts at: 721 2620 2868 721
Size: 1899 248 3044
Fragments arranged by size:
3044 1899 248
NcoI C'CATG_G
Cuts at: 1326 1669 1326
Size: 343 4848
NdeI CA'TA_TG
Cuts at: 1968 1968
Size: 5191
NgoMIV G'CCGG_C
Cuts at: 285 648 3368 285
Size: 363 2720 2108
Fragments arranged by size:
2720 2108 363
NruI TCG'CGA
Cuts at: 3552 3552
Size: 5191
NspI r_CATG'y
Cuts at: 2242 2742 3651 2242
Size: 500 909 3782
Fragments arranged by size:
3782 909 500
PasI CC'CwG_GG
Cuts at: 899 899
Size: 5191
PciI A'CATG_T
Cuts at: 3647 3647
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 899 1073 899
Size: 174 5017
PfoI T'CCnGG_A
Cuts at: 1072 1248 1313 1072
Size: 176 65 4950
Fragments arranged by size:
4950 176 65
PmeI GTTT'AAAC
Cuts at: 2188 2188
Size: 5191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4330 4362 4330
Size: 32 5159
PpuMI rG'GwC_Cy
Cuts at: 924 924
Size: 5191
PstI C_TGCA'G
Cuts at: 914 2063 2236 2921 914
Size: 1149 173 685 3184
Fragments arranged by size:
3184 1149 685 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5191
PvuII CAG'CTG
Cuts at: 848 2614 2974 848
Size: 1766 360 3065
Fragments arranged by size:
3065 1766 360
RsrII CG'GwC_CG
Cuts at: 1097 3384 1097
Size: 2287 2904
SacI G_AGCT'C
Cuts at: 2203 2203
Size: 5191
SacII CC_GC'GG
Cuts at: 416 749 416
Size: 333 4858
SalI G'TCGA_C
Cuts at: 2226 2226
Size: 5191
SanDI GG'GwC_CC
Cuts at: 924 924
Size: 5191
SapI GCTCTTCn'nnn_
Cuts at: 535 3212 3422 535
Size: 2677 210 2304
Fragments arranged by size:
2677 2304 210
SbfI CC_TGCA'GG
Cuts at: 914 2236 914
Size: 1322 3869
ScaI AGT'ACT
Cuts at: 4708 4708
Size: 5191
SexAI A'CCwGG_T
Cuts at: 454 454
Size: 5191
SfcI C'TryA_G
Cuts at: 33 910 2059 2232 2917 3912 4103 33
Size: 877 1149 173 685 995 191 1121
Fragments arranged by size:
1149 1121 995 877 685 191 173
SfoI GGC'GCC
Cuts at: 722 2621 2869 722
Size: 1899 248 3044
Fragments arranged by size:
3044 1899 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
SmaI CCC'GGG
Cuts at: 2211 2211
Size: 5191
SmlI C'TyrA_G
Cuts at: 366 1353 2344 3259 3753 4015 366
Size: 987 991 915 494 262 1542
Fragments arranged by size:
1542 991 987 915 494 262
SnaBI TAC'GTA
Cuts at: 2181 2181
Size: 5191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5191
SphI G_CATG'C
Cuts at: 2242 2742 2242
Size: 500 4691
SspI AAT'ATT
Cuts at: 1263 1263
Size: 5191
StyI C'CwwG_G
Cuts at: 1326 1669 2323 2677 1326
Size: 343 654 354 3840
Fragments arranged by size:
3840 654 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 943 949 1114 2772 2905 3364 3575 943
Size: 6 165 1658 133 459 211 2559
Fragments arranged by size:
2559 1658 459 211 165 133 6
TatI w'GTAC_w
Cuts at: 1572 4706 1572
Size: 3134 2057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1665 1697 4708 4740 1665
Size: 32 3011 32 2116
Fragments arranged by size:
3011 2116 32 32
Tth111I GACn'n_nGTC
Cuts at: 2986 2986
Size: 5191
XbaI T'CTAG_A
Cuts at: 1402 2220 1402
Size: 818 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1074 2684 1074
Size: 1610 3581
XmaI C'CCGG_G
Cuts at: 2209 2209
Size: 5191
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmtI BsaXI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NheI NotI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SfiI SgrAI SrfI StuI
SwaI XhoI XmnI ZraI