Restriction map for
pF1KSDA0941
(Circular) (Six-base) MAPSORT of: pF1KSDA0941.seq Check: 7504 from: 1 to: 4675
pF1KSDA0941 4675 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 414 414
Size: 4675
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4675
Acc65I G'GTAC_C
Cuts at: 220 1689 220
Size: 1469 3206
AclI AA'CG_TT
Cuts at: 4515 4515
Size: 4675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 868 2498 2930 3679 3947 220
Size: 648 1630 432 749 268 948
Fragments arranged by size:
1630 948 749 648 432 268
AflIII A'CryG_T
Cuts at: 2025 3131 4108 2025
Size: 1106 977 2592
Fragments arranged by size:
2592 1106 977
AgeI A'CCGG_T
Cuts at: 3063 3063
Size: 4675
AlwNI CAG_nnn'CTG
Cuts at: 1503 1978 3547 1503
Size: 475 1569 2631
Fragments arranged by size:
2631 1569 475
ApaLI G'TGCA_C
Cuts at: 3445 3445
Size: 4675
ApoI r'AATT_y
Cuts at: 412 721 1066 1134 1357 1648 1677 4028 4284
Size: 309 345 68 223 291 29 2351 256
Cuts at: 4284 412
Size: 803
Fragments arranged by size:
2351 803 345 309 291 256 223 68
29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4675
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4675
AvaI C'yCGr_G
Cuts at: 1555 1693 1555
Size: 138 4537
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4675
BanI G'GyrC_C
Cuts at: 220 856 1689 2103 2351 2386 220
Size: 636 833 414 248 35 2509
Fragments arranged by size:
2509 833 636 414 248 35
BanII G_rGCy'C
Cuts at: 1687 2717 1687
Size: 1030 3645
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4427
BbsI GAAGACnn'nnnn_
Cuts at: 668 827 1026 1424 668
Size: 159 199 398 3919
Fragments arranged by size:
3919 398 199 159
BbvCI CC'TCA_GC
Cuts at: 782 782
Size: 4675
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3334 4675 2571
Size: 763 1341 2571
Fragments arranged by size:
2571 1341 763
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4675
BfrBI ATG'CAT
Cuts at: 1297 1297
Size: 4675
BglII A'GATC_T
Cuts at: 195 607 1237 2188 4227 195
Size: 412 630 951 2039 643
Fragments arranged by size:
2039 951 643 630 412
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4675
Bme1580I G_kGCm'C
Cuts at: 2298 2391 3449 2298
Size: 93 1058 3524
Fragments arranged by size:
3524 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1598 1885 1966 2301 4447 1598
Size: 287 81 335 2146 1826
Fragments arranged by size:
2146 1826 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 333 3040 333
Size: 2707 1968
Bpu10I CC'TnA_GC
Cuts at: 782 782
Size: 4675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1849 2728 3222 3520 313
Size: 1536 879 494 298 1468
Fragments arranged by size:
1536 1468 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4379 4379
Size: 4675
BsaAI yAC'GTr
Cuts at: 168 1665 2026 168
Size: 1497 361 2817
Fragments arranged by size:
2817 1497 361
BsaBI GATnn'nnATC
Cuts at: 6 492 2187 4226 6
Size: 486 1695 2039 455
Fragments arranged by size:
2039 1695 486 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3056 4552 2104
Size: 248 704 1496 2227
Fragments arranged by size:
2227 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3063 3337 3484 3895 1862
Size: 521 680 274 147 411 2642
Fragments arranged by size:
2642 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 594 624 594
Size: 30 4645
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 4675
BseYI C'CCAG_C
Cuts at: 249 2098 2856 3435 3931 249
Size: 1849 758 579 496 993
Fragments arranged by size:
1849 993 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4675
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3471 104
Size: 2111 46 1210 1308
Fragments arranged by size:
2111 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 185 1687 2465 2655 3449 185
Size: 1502 778 190 794 1411
Fragments arranged by size:
1502 1411 794 778 190
BsmI GAATG_Cn'
Cuts at: 801 801
Size: 4675
Bsp1286I G_dGCh'C
Cuts at: 185 1687 2298 2391 2465 2655 2717 3449 185
Size: 1502 611 93 74 190 62 732 1411
Fragments arranged by size:
1502 1411 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3895 3895
Size: 4675
BspHI T'CATG_A
Cuts at: 3851 3851
Size: 4675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 414 1723 1937 2239 2620 162
Size: 252 1309 214 302 381 2217
Fragments arranged by size:
2217 1309 381 302 252 214
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4505 4670 50
Size: 2915 54 1486 165 55
Fragments arranged by size:
2915 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1419 2585 1419
Size: 1166 3509
BsrFI r'CCGG_y
Cuts at: 2671 2852 3063 2671
Size: 181 211 4283
Fragments arranged by size:
4283 211 181
BssSI C'ACGA_G
Cuts at: 2944 3304 2944
Size: 360 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4675
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4675
BstYI r'GATC_y
Cuts at: 1 195 607 1237 1261 1698 2165 2188 2523
Size: 194 412 630 24 437 467 23 335
Cuts at: 2523 2769 3783 3869 3881 4227 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 630 467 449 437 412 346 335
246 194 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 1423 1525 1423
Size: 102 4573
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4331
Fragments arranged by size:
4331 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 1457 1916 1075
Size: 382 459 3834
Fragments arranged by size:
3834 459 382
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4675
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3239 2379
Size: 860 3815
EaeI y'GGCC_r
Cuts at: 2258 2432 2823 2850 4197 2258
Size: 174 391 27 1347 2736
Fragments arranged by size:
2736 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4675
EarI CTCTTCn'nnn_
Cuts at: 1481 2696 2906 1481
Size: 1215 210 3250
Fragments arranged by size:
3250 1215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3193 3339 4485 2881
Size: 312 146 1146 3071
Fragments arranged by size:
3071 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 868 2498 2930 3679 3947 220
Size: 648 1630 432 749 268 948
Fragments arranged by size:
1630 948 749 648 432 268
EcoO109I rG'GnC_Cy
Cuts at: 392 778 824 1812 392
Size: 386 46 988 3255
Fragments arranged by size:
3255 988 386 46
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1540 2692 1540
Size: 1152 3523
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4675
HaeII r_GCGC'y
Cuts at: 2107 2355 3379 4358 2107
Size: 248 1024 979 2424
Fragments arranged by size:
2424 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1302 1318 1350 2515 2545 2547 2577 1270
Size: 32 16 32 1165 30 2 30 3368
Fragments arranged by size:
3368 1165 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1337 1712 1337
Size: 375 4300
HindIII A'AGCT_T
Cuts at: 954 954
Size: 4675
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4427
KpnI G_GTAC'C
Cuts at: 224 1693 224
Size: 1469 3206
MfeI C'AATT_G
Cuts at: 1151 1151
Size: 4675
MluI A'CGCG_T
Cuts at: 4108 4108
Size: 4675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 184 1429 3346 3530 184
Size: 1245 1917 184 1329
Fragments arranged by size:
1917 1329 1245 184
MscI TGG'CCA
Cuts at: 2434 2434
Size: 4675
MslI CAynn'nnrTG
Cuts at: 2662 2789 2662
Size: 127 4548
MspA1I CmG'CkG
Cuts at: 249 1784 2098 2458 3473 3718 249
Size: 1535 314 360 1015 245 1206
Fragments arranged by size:
1535 1206 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4675
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4427
NdeI CA'TA_TG
Cuts at: 830 1397 1623 830
Size: 567 226 3882
Fragments arranged by size:
3882 567 226
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4675
NruI TCG'CGA
Cuts at: 3036 3036
Size: 4675
NsiI A_TGCA'T
Cuts at: 1299 1299
Size: 4675
NspI r_CATG'y
Cuts at: 705 1726 2226 3135 705
Size: 1021 500 909 2245
Fragments arranged by size:
2245 1021 909 500
PciI A'CATG_T
Cuts at: 3131 3131
Size: 4675
PflMI CCAn_nnn'nTGG
Cuts at: 919 919
Size: 4675
PfoI T'CCnGG_A
Cuts at: 1433 1547 1633 1433
Size: 114 86 4475
Fragments arranged by size:
4475 114 86
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4675
PmlI CAC'GTG
Cuts at: 168 168
Size: 4675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3814 3846 3814
Size: 32 4643
PpuMI rG'GwC_Cy
Cuts at: 392 778 824 392
Size: 386 46 4243
Fragments arranged by size:
4243 386 46
PshAI GACnn'nnGTC
Cuts at: 792 792
Size: 4675
PspXI vC'TCGA_Gb
Cuts at: 1555 1555
Size: 4675
PstI C_TGCA'G
Cuts at: 1464 1720 2405 1464
Size: 256 685 3734
Fragments arranged by size:
3734 685 256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4675
PvuII CAG'CTG
Cuts at: 249 2098 2458 249
Size: 1849 360 2466
Fragments arranged by size:
2466 1849 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4675
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4675
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4675
SanDI GG'GwC_CC
Cuts at: 824 824
Size: 4675
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4465
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4675
ScaI AGT'ACT
Cuts at: 265 696 4192 265
Size: 431 3496 748
Fragments arranged by size:
3496 748 431
SfcI C'TryA_G
Cuts at: 33 275 1460 1495 1716 2401 3396 3587 33
Size: 242 1185 35 221 685 995 191 1121
Fragments arranged by size:
1185 1121 995 685 242 221 191 35
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4427
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4675
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4675
SmlI C'TyrA_G
Cuts at: 292 1555 1828 2743 3237 3499 292
Size: 1263 273 915 494 262 1468
Fragments arranged by size:
1468 1263 915 494 273 262
SnaBI TAC'GTA
Cuts at: 1665 1665
Size: 4675
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4675
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4175
SspI AAT'ATT
Cuts at: 543 543
Size: 4675
StuI AGG'CCT
Cuts at: 316 316
Size: 4675
StyI C'CwwG_G
Cuts at: 1081 1807 2161 1081
Size: 726 354 3595
Fragments arranged by size:
3595 726 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1555 2256 2389 2848 3059 1555
Size: 701 133 459 211 3171
Fragments arranged by size:
3171 701 459 211 133
TatI w'GTAC_w
Cuts at: 263 694 4190 263
Size: 431 3496 748
Fragments arranged by size:
3496 748 431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4192 4224 4192
Size: 32 4643
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4675
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4675
XhoI C'TCGA_G
Cuts at: 1555 1555
Size: 4675
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4675
XmnI GAAnn'nnTTC
Cuts at: 663 1490 663
Size: 827 3848
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NruI NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BpmI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NheI NotI PacI PasI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SrfI SwaI ZraI