Restriction map for
pF1KSDA0945
(Circular) (Six-base) MAPSORT of: pF1KSDA0945.seq Check: 2366 from: 1 to: 5474
pF1KSDA0945 5474 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1073 1140 1668 1073
Size: 67 528 4879
Fragments arranged by size:
4879 528 67
AccI GT'mk_AC
Cuts at: 2512 2512
Size: 5474
Acc65I G'GTAC_C
Cuts at: 1896 2490 1896
Size: 594 4880
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1117 1669 3299 3731 4478 4746 518
Size: 599 552 1630 432 747 268 1246
Fragments arranged by size:
1630 1246 747 599 552 432 268
AflII C'TTAA_G
Cuts at: 2449 2449
Size: 5474
AflIII A'CryG_T
Cuts at: 1842 2826 3930 4907 1842
Size: 984 1104 977 2409
Fragments arranged by size:
2409 1104 984 977
AgeI A'CCGG_T
Cuts at: 3862 3862
Size: 5474
AleI CACnn'nnGTG
Cuts at: 1459 1459
Size: 5474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 358 1575 358
Size: 1217 4257
AlwNI CAG_nnn'CTG
Cuts at: 1149 1348 1478 2279 2779 4346 1149
Size: 199 130 801 500 1567 2277
Fragments arranged by size:
2277 1567 801 500 199 130
ApaLI G'TGCA_C
Cuts at: 4244 4244
Size: 5474
ApoI r'AATT_y
Cuts at: 1582 2478 4827 5083 1582
Size: 896 2349 256 1973
Fragments arranged by size:
2349 1973 896 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 250 858 2089 2494 250
Size: 608 1231 405 3230
Fragments arranged by size:
3230 1231 608 405
BamHI G'GATC_C
Cuts at: 573 2499 573
Size: 1926 3548
BanI G'GyrC_C
Cuts at: 275 476 550 1112 1399 1637 1896 2490 2904
Size: 201 74 562 287 238 259 594 414
Cuts at: 2904 3152 3187 275
Size: 248 35 2562
Fragments arranged by size:
2562 594 562 414 287 259 248 238
201 74 35
BanII G_rGCy'C
Cuts at: 191 259 842 1045 1244 1370 1387 1429 1673
Size: 68 583 203 199 126 17 42 244
Cuts at: 1673 1706 2177 2488 3518 191
Size: 33 471 311 1030 2147
Fragments arranged by size:
2147 1030 583 471 311 244 203 199
126 68 42 33 17
BbeI G_GCGC'C
Cuts at: 279 480 1116 2908 3156 279
Size: 201 636 1792 248 2597
Fragments arranged by size:
2597 1792 636 248 201
BbsI GAAGACnn'nnnn_
Cuts at: 209 452 209
Size: 243 5231
BbvCI CC'TCA_GC
Cuts at: 1175 1175
Size: 5474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1371 1405 1371
Size: 34 5440
BciVI GTATCCnnnnn_n'
Cuts at: 3372 4133 5474 3372
Size: 761 1341 3372
Fragments arranged by size:
3372 1341 761
BclI T'GATC_A
Cuts at: 2994 2994
Size: 5474
BglI GCCn_nnn'nGGC
Cuts at: 176 548 870 944 176
Size: 372 322 74 4706
Fragments arranged by size:
4706 372 322 74
BglII A'GATC_T
Cuts at: 2066 2989 5026 2066
Size: 923 2037 2514
Fragments arranged by size:
2514 2037 923
BlpI GC'TnA_GC
Cuts at: 2586 2586
Size: 5474
Bme1580I G_kGCm'C
Cuts at: 810 1394 1642 1729 3099 3192 4248 810
Size: 584 248 87 1370 93 1056 2036
Fragments arranged by size:
2036 1370 1056 584 248 93 87
BmrI ACTGGGnnnn_n'
Cuts at: 617 1336 1643 2454 2686 2767 3102 5246 617
Size: 719 307 811 232 81 335 2144 845
Fragments arranged by size:
2144 845 811 719 335 307 232 81
BmtI G_CTAG'C
Cuts at: 743 743
Size: 5474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1049 3841 1049
Size: 2792 2682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 541 662 1057 1478 1764 376
Size: 165 121 395 421 286 4086
Fragments arranged by size:
4086 421 395 286 165 121
Bpu10I CC'TnA_GC
Cuts at: 1024 1139 1175 1238 2135 2396 1024
Size: 115 36 63 897 261 4102
Fragments arranged by size:
4102 897 261 115 63 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2650 3529 4021 4319 2650
Size: 879 492 298 3805
Fragments arranged by size:
3805 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 710 5178 710
Size: 4468 1006
BsaAI yAC'GTr
Cuts at: 1843 2466 2827 1843
Size: 623 361 4490
Fragments arranged by size:
4490 623 361
BsaBI GATnn'nnATC
Cuts at: 6 2988 5025 6
Size: 2982 2037 455
Fragments arranged by size:
2982 2037 455
BsaHI Gr'CG_yC
Cuts at: 276 477 1113 2905 3153 3855 5351 276
Size: 201 636 1792 248 702 1496 399
Fragments arranged by size:
1792 1496 702 636 399 248 201
BsaWI w'CCGG_w
Cuts at: 2663 3184 3862 4136 4283 4694 2663
Size: 521 678 274 147 411 3443
Fragments arranged by size:
3443 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 612 671 1138 1360 1375 1419 2251 612
Size: 59 467 222 15 44 832 3835
Fragments arranged by size:
3835 832 467 222 59 44 15
BseYI C'CCAG_C
Cuts at: 424 461 642 720 843 1608 1747 1892 2161
Size: 37 181 78 123 765 139 145 269
Cuts at: 2161 2314 2899 3657 4234 4730 424
Size: 153 585 758 577 496 1168
Fragments arranged by size:
1168 765 758 585 577 496 269 181
153 145 139 123 78 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1232 1334 401
Size: 831 102 4541
Fragments arranged by size:
4541 831 102
BsiEI CG_ry'CG
Cuts at: 104 3016 3062 4270 104
Size: 2912 46 1208 1308
Fragments arranged by size:
2912 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1244 1387 2141 2488 3266 3456 4248 1244
Size: 143 754 347 778 190 792 2470
Fragments arranged by size:
2470 792 778 754 347 190 143
Bsp1286I G_dGCh'C
Cuts at: 191 259 810 842 1045 1244 1370 1387 1394
Size: 68 551 32 203 199 126 17 7
Cuts at: 1394 1429 1642 1673 1706 1729 2141 2177 2488
Size: 35 213 31 33 23 412 36 311
Cuts at: 2488 3099 3192 3266 3456 3518 4248 191
Size: 611 93 74 190 62 730 1417
Fragments arranged by size:
1417 730 611 551 412 311 213 203
199 190 126 93 74 68 62 36
35 33 32 31 23 17 7
BspEI T'CCGG_A
Cuts at: 4694 4694
Size: 5474
BspHI T'CATG_A
Cuts at: 4650 4650
Size: 5474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 521 1073 1140 1668 2264 2524 2738 3040
Size: 102 552 67 528 596 260 214 302
Cuts at: 3040 3421 419
Size: 381 2472
Fragments arranged by size:
2472 596 552 528 381 302 260 214
102 67
BsrBI CCG'CTC
Cuts at: 50 780 1983 3766 3820 5304 5469 50
Size: 730 1203 1783 54 1484 165 55
Fragments arranged by size:
1783 1484 1203 730 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3386 3386
Size: 5474
BsrFI r'CCGG_y
Cuts at: 367 3472 3653 3862 367
Size: 3105 181 209 1979
Fragments arranged by size:
3105 1979 209 181
BssSI C'ACGA_G
Cuts at: 803 1357 2170 3745 4103 803
Size: 554 813 1575 358 2174
Fragments arranged by size:
2174 1575 813 554 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1149 2851 1149
Size: 1702 3772
BstBI TT'CG_AA
Cuts at: 110 3835 110
Size: 3725 1749
BstEII G'GTnAC_C
Cuts at: 262 262
Size: 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 543 735 947 543
Size: 192 212 5070
Fragments arranged by size:
5070 212 192
BstYI r'GATC_y
Cuts at: 1 573 1564 2066 2499 2966 2989 3324 3570
Size: 572 991 502 433 467 23 335 246
Cuts at: 3570 4582 4668 4680 5026 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 991 572 502 467 449 433 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1325 1395 1325
Size: 70 5404
BtgI C'CryG_G
Cuts at: 970 2285 2436 970
Size: 1315 151 4008
Fragments arranged by size:
4008 1315 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2183 3424 3605 3768 2183
Size: 1241 181 163 3889
Fragments arranged by size:
3889 1241 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1297 1793 2717 1244
Size: 53 496 924 4001
Fragments arranged by size:
4001 924 496 53
DraI TTT'AAA
Cuts at: 2473 2473
Size: 5474
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4038 3180
Size: 858 4616
EaeI y'GGCC_r
Cuts at: 177 365 973 1275 1602 2060 2288 3059 3233
Size: 188 608 302 327 458 228 771 174
Cuts at: 3233 3624 3651 4996 177
Size: 391 27 1345 655
Fragments arranged by size:
1345 771 655 608 458 391 327 302
228 188 174 27
EagI C'GGCC_G
Cuts at: 3059 3059
Size: 5474
EarI CTCTTCn'nnn_
Cuts at: 3497 3707 3497
Size: 210 5264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 3682 3992 4138 5284 2422
Size: 1260 310 146 1146 2612
Fragments arranged by size:
2612 1260 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1242 1385 2486 1242
Size: 143 1101 4230
Fragments arranged by size:
4230 1101 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 518 541 662 1057 1117 1478 1669 1764
Size: 142 23 121 395 60 361 191 95
Cuts at: 1764 3299 3731 4478 4746 376
Size: 1535 432 747 268 1104
Fragments arranged by size:
1535 1104 747 432 395 361 268 191
142 121 95 60 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1027 1027
Size: 5474
EcoO109I rG'GnC_Cy
Cuts at: 648 669 700 855 2613 648
Size: 21 31 155 1758 3509
Fragments arranged by size:
3509 1758 155 31 21
EcoRI G'AATT_C
Cuts at: 2478 2478
Size: 5474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5474
FspI TGC'GCA
Cuts at: 1771 3255 1771
Size: 1484 3990
HaeII r_GCGC'y
Cuts at: 279 306 480 831 1116 1739 1854 2298 2908
Size: 27 174 351 285 623 115 444 610
Cuts at: 2908 3156 4178 5157 279
Size: 248 1022 979 596
Fragments arranged by size:
1022 979 623 610 596 444 351 285
248 174 115 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 246 278 814 846 3316 3346 3348
Size: 32 94 32 536 32 2470 30 2
Cuts at: 3348 3378 120
Size: 30 2216
Fragments arranged by size:
2470 2216 536 94 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2513 2513
Size: 5474
KasI G'GCGC_C
Cuts at: 275 476 1112 2904 3152 275
Size: 201 636 1792 248 2597
Fragments arranged by size:
2597 1792 636 248 201
KpnI G_GTAC'C
Cuts at: 1900 2494 1900
Size: 594 4880
MluI A'CGCG_T
Cuts at: 4907 4907
Size: 5474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1559 4145 4329 1559
Size: 2586 184 2704
Fragments arranged by size:
2704 2586 184
MscI TGG'CCA
Cuts at: 975 1277 1604 2062 2290 3235 975
Size: 302 327 458 228 945 3214
Fragments arranged by size:
3214 945 458 327 302 228
MslI CAynn'nnrTG
Cuts at: 1459 2423 3463 3590 1459
Size: 964 1040 127 3343
Fragments arranged by size:
3343 1040 964 127
MspA1I CmG'CkG
Cuts at: 754 1179 1188 1751 1784 2585 2899 3259 4272
Size: 425 9 563 33 801 314 360 1013
Cuts at: 4272 4517 754
Size: 245 1711
Fragments arranged by size:
1711 1013 801 563 425 360 314 245
33 9
NaeI GCC'GGC
Cuts at: 3655 3655
Size: 5474
NarI GG'CG_CC
Cuts at: 276 477 1113 2905 3153 276
Size: 201 636 1792 248 2597
Fragments arranged by size:
2597 1792 636 248 201
NcoI C'CATG_G
Cuts at: 970 2285 970
Size: 1315 4159
NgoMIV G'CCGG_C
Cuts at: 3653 3653
Size: 5474
NheI G'CTAG_C
Cuts at: 739 739
Size: 5474
NruI TCG'CGA
Cuts at: 2202 2202
Size: 5474
NspI r_CATG'y
Cuts at: 249 2169 2527 3027 3934 249
Size: 1920 358 500 907 1789
Fragments arranged by size:
1920 1789 907 500 358
PasI CC'CwG_GG
Cuts at: 1421 1569 1421
Size: 148 5326
PciI A'CATG_T
Cuts at: 3930 3930
Size: 5474
PflMI CCAn_nnn'nTGG
Cuts at: 905 905
Size: 5474
PfoI T'CCnGG_A
Cuts at: 1592 2006 1592
Size: 414 5060
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PmlI CAC'GTG
Cuts at: 1843 1843
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2421 4613 4645 2389
Size: 32 2192 32 3218
Fragments arranged by size:
3218 2192 32 32
PpuMI rG'GwC_Cy
Cuts at: 648 669 700 648
Size: 21 31 5422
Fragments arranged by size:
5422 31 21
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2260 2292 2446 2478 2260
Size: 32 154 32 5256
Fragments arranged by size:
5256 154 32 32
PstI C_TGCA'G
Cuts at: 197 936 1292 1564 1665 1789 2275 2349 2521
Size: 739 356 272 101 124 486 74 172
Cuts at: 2521 3206 197
Size: 685 2465
Fragments arranged by size:
2465 739 685 486 356 272 172 124
101 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 754 1179 1784 2899 3259 754
Size: 425 605 1115 360 2969
Fragments arranged by size:
2969 1115 605 425 360
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 1244 1387 2488 1244
Size: 143 1101 4230
Fragments arranged by size:
4230 1101 143
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 3497 3707 3497
Size: 210 5264
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 2269 4991 2269
Size: 2722 2752
SfcI C'TryA_G
Cuts at: 33 193 932 1288 1470 1560 1661 1785 2271
Size: 160 739 356 182 90 101 124 486
Cuts at: 2271 2345 2517 3202 4195 4386 33
Size: 74 172 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 486 356 191 182
172 160 124 101 90 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 176 870 176
Size: 694 4780
SfoI GGC'GCC
Cuts at: 277 478 1114 2906 3154 277
Size: 201 636 1792 248 2597
Fragments arranged by size:
2597 1792 636 248 201
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 2496 2496
Size: 5474
SmlI C'TyrA_G
Cuts at: 2449 2629 3544 4036 4298 2449
Size: 180 915 492 262 3625
Fragments arranged by size:
3625 915 492 262 180
SnaBI TAC'GTA
Cuts at: 2466 2466
Size: 5474
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5474
SphI G_CATG'C
Cuts at: 249 2169 2527 3027 249
Size: 1920 358 500 2696
Fragments arranged by size:
2696 1920 500 358
StuI AGG'CCT
Cuts at: 331 1821 2309 331
Size: 1490 488 3496
Fragments arranged by size:
3496 1490 488
StyI C'CwwG_G
Cuts at: 326 651 851 875 899 970 2285 2608 2962
Size: 325 200 24 24 71 1315 323 354
Cuts at: 2962 326
Size: 2838
Fragments arranged by size:
2838 1315 354 325 323 200 71 24
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 864 1069 1622 1640 1707 3057 3190 3649 3858
Size: 205 553 18 67 1350 133 459 209
Cuts at: 3858 864
Size: 2480
Fragments arranged by size:
2480 1350 553 459 209 205 133 67
18
TatI w'GTAC_w
Cuts at: 1268 2267 4989 1268
Size: 999 2722 1753
Fragments arranged by size:
2722 1753 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4991 5023 4991
Size: 32 5442
Tth111I GACn'n_nGTC
Cuts at: 2014 3271 2014
Size: 1257 4217
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2055 2969 2055
Size: 914 4560
XmaI C'CCGG_G
Cuts at: 2494 2494
Size: 5474
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BfrBI BmgBI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NsiI PacI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI