(Circular) (Six-base) MAPSORT of: pF1KSDA0948.seq Check: 3228 from: 1 to: 4702 pF1KSDA0948 4702 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 327 1011 327 Size: 684 4018 AatII G_ACGT'C Cuts at: 1592 1592 Size: 4702 AccI GT'mk_AC Cuts at: 1738 1738 Size: 4702 Acc65I G'GTAC_C Cuts at: 556 1716 556 Size: 1160 3542 AclI AA'CG_TT Cuts at: 4542 4542 Size: 4702 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 948 1315 2525 2957 3706 3974 948 Size: 367 1210 432 749 268 1676 Fragments arranged by size: 1676 1210 749 432 367 268 AflIII A'CryG_T Cuts at: 2052 3158 4135 2052 Size: 1106 977 2619 Fragments arranged by size: 2619 1106 977 AgeI A'CCGG_T Cuts at: 3090 3090 Size: 4702 AleI CACnn'nnGTG Cuts at: 796 796 Size: 4702 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1543 1543 Size: 4702 AlwNI CAG_nnn'CTG Cuts at: 732 1155 2005 3574 732 Size: 423 850 1569 1860 Fragments arranged by size: 1860 1569 850 423 ApaI G_GGCC'C Cuts at: 1041 1241 1041 Size: 200 4502 ApaLI G'TGCA_C Cuts at: 3472 3472 Size: 4702 ApoI r'AATT_y Cuts at: 1096 1704 4055 4311 1096 Size: 608 2351 256 1487 Fragments arranged by size: 2351 1487 608 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4702 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4702 AvaI C'yCGr_G Cuts at: 1192 1200 1330 1720 1192 Size: 8 130 390 4174 Fragments arranged by size: 4174 390 130 8 BamHI G'GATC_C Cuts at: 1725 1725 Size: 4702 BanI G'GyrC_C Cuts at: 556 1419 1636 1716 2130 2378 2413 556 Size: 863 217 80 414 248 35 2845 Fragments arranged by size: 2845 863 414 248 217 80 35 BanII G_rGCy'C Cuts at: 380 1041 1241 1714 2744 380 Size: 661 200 473 1030 2338 Fragments arranged by size: 2338 1030 661 473 200 BbeI G_GCGC'C Cuts at: 1640 2134 2382 1640 Size: 494 248 3960 Fragments arranged by size: 3960 494 248 BbsI GAAGACnn'nnnn_ Cuts at: 860 860 Size: 4702 BbvCI CC'TCA_GC Cuts at: 249 1242 1367 1673 249 Size: 993 125 306 3278 Fragments arranged by size: 3278 993 306 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 137 171 137 Size: 34 4668 BciVI GTATCCnnnnn_n' Cuts at: 2598 3361 4702 2598 Size: 763 1341 2598 Fragments arranged by size: 2598 1341 763 BclI T'GATC_A Cuts at: 2220 2220 Size: 4702 BglI GCCn_nnn'nGGC Cuts at: 685 685 Size: 4702 BglII A'GATC_T Cuts at: 2215 4254 2215 Size: 2039 2663 BlpI GC'TnA_GC Cuts at: 740 1812 740 Size: 1072 3630 Bme1580I G_kGCm'C Cuts at: 1041 1241 2325 2418 3476 1041 Size: 200 1084 93 1058 2267 Fragments arranged by size: 2267 1084 1058 200 93 BmgBI CAC'GTC Cuts at: 864 864 Size: 4702 BmrI ACTGGGnnnn_n' Cuts at: 1451 1912 1993 2328 4474 1451 Size: 461 81 335 2146 1679 Fragments arranged by size: 2146 1679 461 335 81 BmtI G_CTAG'C Cuts at: 1480 1480 Size: 4702 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 738 3067 738 Size: 2329 2373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 182 403 1027 1204 1445 182 Size: 221 624 177 241 3439 Fragments arranged by size: 3439 624 241 221 177 Bpu10I CC'TnA_GC Cuts at: 249 1242 1367 1673 249 Size: 993 125 306 3278 Fragments arranged by size: 3278 993 306 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 646 1600 1630 1876 2755 3249 3547 646 Size: 954 30 246 879 494 298 1801 Fragments arranged by size: 1801 954 879 494 298 246 30 BsaI GGTCTCn'nnnn_ Cuts at: 4406 4406 Size: 4702 BsaAI yAC'GTr Cuts at: 155 1692 2053 155 Size: 1537 361 2804 Fragments arranged by size: 2804 1537 361 BsaBI GATnn'nnATC Cuts at: 6 2214 4253 6 Size: 2208 2039 455 Fragments arranged by size: 2208 2039 455 BsaHI Gr'CG_yC Cuts at: 1589 1637 2131 2379 3083 4579 1589 Size: 48 494 248 704 1496 1712 Fragments arranged by size: 1712 1496 704 494 248 48 BsaWI w'CCGG_w Cuts at: 1889 2410 3090 3364 3511 3922 1889 Size: 521 680 274 147 411 2669 Fragments arranged by size: 2669 680 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 607 637 607 Size: 30 4672 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 388 1690 388 Size: 1302 3400 BseYI C'CCAG_C Cuts at: 573 984 1305 1352 1433 1530 2125 2883 3462 Size: 411 321 47 81 97 595 758 579 Cuts at: 3462 3958 573 Size: 496 1317 Fragments arranged by size: 1317 758 595 579 496 411 321 97 81 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 924 924 Size: 4702 BsiEI CG_ry'CG Cuts at: 104 2242 2288 3498 104 Size: 2138 46 1210 1308 Fragments arranged by size: 2138 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 380 1714 2492 2682 3476 380 Size: 1334 778 190 794 1606 Fragments arranged by size: 1606 1334 794 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1282 1282 Size: 4702 Bsp1286I G_dGCh'C Cuts at: 380 1041 1241 1714 2325 2418 2492 2682 2744 Size: 661 200 473 611 93 74 190 62 Cuts at: 2744 3476 380 Size: 732 1606 Fragments arranged by size: 1606 732 661 611 473 200 190 93 74 62 BspEI T'CCGG_A Cuts at: 3922 3922 Size: 4702 BspHI T'CATG_A Cuts at: 806 3878 806 Size: 3072 1630 BspMI ACCTGCnnnn'nnnn_ Cuts at: 327 1011 1750 1964 2266 2647 327 Size: 684 739 214 302 381 2382 Fragments arranged by size: 2382 739 684 381 302 214 BsrBI CCG'CTC Cuts at: 50 2992 3046 4532 4697 50 Size: 2942 54 1486 165 55 Fragments arranged by size: 2942 1486 165 55 54 BsrDI GCAATG_nn' Cuts at: 434 2612 434 Size: 2178 2524 BsrFI r'CCGG_y Cuts at: 294 2698 2879 3090 294 Size: 2404 181 211 1906 Fragments arranged by size: 2404 1906 211 181 BssSI C'ACGA_G Cuts at: 2971 3331 2971 Size: 360 4342 BstAPI GCAn_nnn'nTGC Cuts at: 732 2077 732 Size: 1345 3357 BstBI TT'CG_AA Cuts at: 110 110 Size: 4702 BstEII G'GTnAC_C Cuts at: 1260 1260 Size: 4702 BstXI CCAn_nnnn'nTGG Cuts at: 1441 1441 Size: 4702 BstYI r'GATC_y Cuts at: 1 1725 2192 2215 2550 2796 3810 3896 3908 Size: 1724 467 23 335 246 1014 86 12 Cuts at: 3908 4254 1 Size: 346 449 Fragments arranged by size: 1724 1014 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 125 191 125 Size: 66 4636 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 162 2650 2831 2994 162 Size: 2488 181 163 1870 Fragments arranged by size: 2488 1870 181 163 BtsI GCAGTG_nn' Cuts at: 1172 1943 1172 Size: 771 3931 DraI TTT'AAA Cuts at: 1699 1699 Size: 4702 DraIII CAC_nnn'GTG Cuts at: 1563 1563 Size: 4702 DrdI GACnn_nn'nnGTC Cuts at: 862 2406 3266 862 Size: 1544 860 2298 Fragments arranged by size: 2298 1544 860 EaeI y'GGCC_r Cuts at: 204 1337 1682 2285 2459 2850 2877 4224 204 Size: 1133 345 603 174 391 27 1347 682 Fragments arranged by size: 1347 1133 682 603 391 345 174 27 EagI C'GGCC_G Cuts at: 2285 2285 Size: 4702 EarI CTCTTCn'nnn_ Cuts at: 2723 2933 2723 Size: 210 4492 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2908 3220 3366 4512 2908 Size: 312 146 1146 3098 Fragments arranged by size: 3098 1146 312 146 EcoICRI GAG'CTC Cuts at: 378 1712 378 Size: 1334 3368 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 182 403 948 1027 1204 1315 1445 2525 2957 Size: 221 545 79 177 111 130 1080 432 Cuts at: 2957 3706 3974 182 Size: 749 268 910 Fragments arranged by size: 1080 910 749 545 432 268 221 177 130 111 79 EcoNI CCTnn'n_nnAGG Cuts at: 709 1273 709 Size: 564 4138 EcoO109I rG'GnC_Cy Cuts at: 183 1038 1204 1238 1839 183 Size: 855 166 34 601 3046 Fragments arranged by size: 3046 855 601 166 34 EcoRI G'AATT_C Cuts at: 1704 1704 Size: 4702 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 2719 Size: 4702 FspI TGC'GCA Cuts at: 1660 2481 1660 Size: 821 3881 HaeII r_GCGC'y Cuts at: 1316 1640 2134 2382 3406 4385 1316 Size: 324 494 248 1024 979 1633 Fragments arranged by size: 1633 1024 979 494 324 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 607 639 2542 2572 2574 2604 607 Size: 32 1903 30 2 30 2705 Fragments arranged by size: 2705 1903 32 30 30 2 HincII GTy'rAC Cuts at: 1739 1739 Size: 4702 KasI G'GCGC_C Cuts at: 1636 2130 2378 1636 Size: 494 248 3960 Fragments arranged by size: 3960 494 248 KpnI G_GTAC'C Cuts at: 560 1720 560 Size: 1160 3542 MluI A'CGCG_T Cuts at: 4135 4135 Size: 4702 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 748 3373 3557 748 Size: 2625 184 1893 Fragments arranged by size: 2625 1893 184 MscI TGG'CCA Cuts at: 1339 1684 2461 1339 Size: 345 777 3580 Fragments arranged by size: 3580 777 345 MslI CAynn'nnrTG Cuts at: 796 2689 2816 796 Size: 1893 127 2682 Fragments arranged by size: 2682 1893 127 MspA1I CmG'CkG Cuts at: 644 1811 2125 2485 3500 3745 644 Size: 1167 314 360 1015 245 1601 Fragments arranged by size: 1601 1167 1015 360 314 245 NaeI GCC'GGC Cuts at: 2881 2881 Size: 4702 NarI GG'CG_CC Cuts at: 1637 2131 2379 1637 Size: 494 248 3960 Fragments arranged by size: 3960 494 248 NcoI C'CATG_G Cuts at: 125 191 125 Size: 66 4636 NgoMIV G'CCGG_C Cuts at: 2879 2879 Size: 4702 NheI G'CTAG_C Cuts at: 1476 1476 Size: 4702 NruI TCG'CGA Cuts at: 3063 3063 Size: 4702 NspI r_CATG'y Cuts at: 654 1753 2253 3162 654 Size: 1099 500 909 2194 Fragments arranged by size: 2194 1099 909 500 PasI CC'CwG_GG Cuts at: 1234 1234 Size: 4702 PciI A'CATG_T Cuts at: 3158 3158 Size: 4702 PfoI T'CCnGG_A Cuts at: 380 380 Size: 4702 PmeI GTTT'AAAC Cuts at: 1699 1699 Size: 4702 PmlI CAC'GTG Cuts at: 155 155 Size: 4702 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 943 975 3841 3873 943 Size: 32 2866 32 1772 Fragments arranged by size: 2866 1772 32 32 PpuMI rG'GwC_Cy Cuts at: 1204 1204 Size: 4702 PspOMI G'GGCC_C Cuts at: 1037 1237 1037 Size: 200 4502 PspXI vC'TCGA_Gb Cuts at: 1330 1330 Size: 4702 PstI C_TGCA'G Cuts at: 623 1022 1055 1286 1572 1747 2432 623 Size: 399 33 231 286 175 685 2893 Fragments arranged by size: 2893 685 399 286 231 175 33 PvuI CG_AT'CG Cuts at: 104 104 Size: 4702 PvuII CAG'CTG Cuts at: 2125 2485 2125 Size: 360 4342 RsrII CG'GwC_CG Cuts at: 2895 2895 Size: 4702 SacI G_AGCT'C Cuts at: 380 1714 380 Size: 1334 3368 SalI G'TCGA_C Cuts at: 1737 1737 Size: 4702 SanDI GG'GwC_CC Cuts at: 1204 1204 Size: 4702 SapI GCTCTTCn'nnn_ Cuts at: 2723 2933 2723 Size: 210 4492 SbfI CC_TGCA'GG Cuts at: 1022 1747 1022 Size: 725 3977 ScaI AGT'ACT Cuts at: 1198 4219 1198 Size: 3021 1681 SexAI A'CCwGG_T Cuts at: 137 1028 137 Size: 891 3811 SfcI C'TryA_G Cuts at: 33 619 1018 1051 1282 1568 1743 2428 3423 Size: 586 399 33 231 286 175 685 995 Cuts at: 3423 3614 33 Size: 191 1121 Fragments arranged by size: 1121 995 685 586 399 286 231 191 175 33 SfoI GGC'GCC Cuts at: 1638 2132 2380 1638 Size: 494 248 3960 Fragments arranged by size: 3960 494 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4702 SmaI CCC'GGG Cuts at: 1722 1722 Size: 4702 SmlI C'TyrA_G Cuts at: 661 1330 1615 1645 1855 2770 3264 3526 661 Size: 669 285 30 210 915 494 262 1837 Fragments arranged by size: 1837 915 669 494 285 262 210 30 SnaBI TAC'GTA Cuts at: 1692 1692 Size: 4702 SpeI A'CTAG_T Cuts at: 67 67 Size: 4702 SphI G_CATG'C Cuts at: 654 1753 2253 654 Size: 1099 500 3103 Fragments arranged by size: 3103 1099 500 StuI AGG'CCT Cuts at: 607 1004 607 Size: 397 4305 StyI C'CwwG_G Cuts at: 125 191 602 611 1834 2188 125 Size: 66 411 9 1223 354 2639 Fragments arranged by size: 2639 1223 411 354 66 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 276 534 1344 2283 2416 2875 3086 276 Size: 258 810 939 133 459 211 1892 Fragments arranged by size: 1892 939 810 459 258 211 133 TatI w'GTAC_w Cuts at: 665 1196 4217 665 Size: 531 3021 1150 Fragments arranged by size: 3021 1150 531 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1018 1050 4219 4251 1018 Size: 32 3169 32 1469 Fragments arranged by size: 3169 1469 32 32 Tth111I GACn'n_nGTC Cuts at: 2497 2497 Size: 4702 XbaI T'CTAG_A Cuts at: 1731 1731 Size: 4702 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1179 1601 2195 1179 Size: 422 594 3686 Fragments arranged by size: 3686 594 422 XhoI C'TCGA_G Cuts at: 1330 1330 Size: 4702 XmaI C'CCGG_G Cuts at: 1720 1720 Size: 4702 XmnI GAAnn'nnTTC Cuts at: 1144 1144 Size: 4702 ZraI GAC'GTC Cuts at: 1590 1590 Size: 4702 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AhdI AloI AscI AvrII BaeI BfrBI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NsiI PacI PflMI PshAI PsiI PsrI SacII SfiI SgrAI SrfI SspI SwaI