Restriction map for pF1KSDA0948
 (Circular) (Six-base) MAPSORT of: pF1KSDA0948.seq  Check: 3228  from: 1  to: 4702



pF1KSDA0948  4702 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    327    1011     327 
   Size:        684    4018
 
AatII G_ACGT'C

Cuts at:   1592    1592 
   Size:       4702
 
AccI GT'mk_AC

Cuts at:   1738    1738 
   Size:       4702
 
Acc65I G'GTAC_C

Cuts at:    556    1716     556 
   Size:       1160    3542
 
AclI AA'CG_TT

Cuts at:   4542    4542 
   Size:       4702
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    948    1315    2525    2957    3706    3974     948 
   Size:        367    1210     432     749     268    1676

  Fragments arranged by size: 

                1676    1210     749     432     367     268
 
AflIII A'CryG_T

Cuts at:   2052    3158    4135    2052 
   Size:       1106     977    2619

  Fragments arranged by size: 

                2619    1106     977
 
AgeI A'CCGG_T

Cuts at:   3090    3090 
   Size:       4702
 
AleI CACnn'nnGTG

Cuts at:    796     796 
   Size:       4702
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1543    1543 
   Size:       4702
 
AlwNI CAG_nnn'CTG

Cuts at:    732    1155    2005    3574     732 
   Size:        423     850    1569    1860

  Fragments arranged by size: 

                1860    1569     850     423
 
ApaI G_GGCC'C

Cuts at:   1041    1241    1041 
   Size:        200    4502
 
ApaLI G'TGCA_C

Cuts at:   3472    3472 
   Size:       4702
 
ApoI r'AATT_y

Cuts at:   1096    1704    4055    4311    1096 
   Size:        608    2351     256    1487

  Fragments arranged by size: 

                2351    1487     608     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4702
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4702
 
AvaI C'yCGr_G

Cuts at:   1192    1200    1330    1720    1192 
   Size:          8     130     390    4174

  Fragments arranged by size: 

                4174     390     130       8
 
BamHI G'GATC_C

Cuts at:   1725    1725 
   Size:       4702
 
BanI G'GyrC_C

Cuts at:    556    1419    1636    1716    2130    2378    2413     556 
   Size:        863     217      80     414     248      35    2845

  Fragments arranged by size: 

                2845     863     414     248     217      80      35
 
BanII G_rGCy'C

Cuts at:    380    1041    1241    1714    2744     380 
   Size:        661     200     473    1030    2338

  Fragments arranged by size: 

                2338    1030     661     473     200
 
BbeI G_GCGC'C

Cuts at:   1640    2134    2382    1640 
   Size:        494     248    3960

  Fragments arranged by size: 

                3960     494     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    860     860 
   Size:       4702
 
BbvCI CC'TCA_GC

Cuts at:    249    1242    1367    1673     249 
   Size:        993     125     306    3278

  Fragments arranged by size: 

                3278     993     306     125
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    137     171     137 
   Size:         34    4668
 
BciVI GTATCCnnnnn_n'

Cuts at:   2598    3361    4702    2598 
   Size:        763    1341    2598

  Fragments arranged by size: 

                2598    1341     763
 
BclI T'GATC_A

Cuts at:   2220    2220 
   Size:       4702
 
BglI GCCn_nnn'nGGC

Cuts at:    685     685 
   Size:       4702
 
BglII A'GATC_T

Cuts at:   2215    4254    2215 
   Size:       2039    2663
 
BlpI GC'TnA_GC

Cuts at:    740    1812     740 
   Size:       1072    3630
 
Bme1580I G_kGCm'C

Cuts at:   1041    1241    2325    2418    3476    1041 
   Size:        200    1084      93    1058    2267

  Fragments arranged by size: 

                2267    1084    1058     200      93
 
BmgBI CAC'GTC

Cuts at:    864     864 
   Size:       4702
 
BmrI ACTGGGnnnn_n'

Cuts at:   1451    1912    1993    2328    4474    1451 
   Size:        461      81     335    2146    1679

  Fragments arranged by size: 

                2146    1679     461     335      81
 
BmtI G_CTAG'C

Cuts at:   1480    1480 
   Size:       4702
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    738    3067     738 
   Size:       2329    2373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     403    1027    1204    1445     182 
   Size:        221     624     177     241    3439

  Fragments arranged by size: 

                3439     624     241     221     177
 
Bpu10I CC'TnA_GC

Cuts at:    249    1242    1367    1673     249 
   Size:        993     125     306    3278

  Fragments arranged by size: 

                3278     993     306     125
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    646    1600    1630    1876    2755    3249    3547     646 
   Size:        954      30     246     879     494     298    1801

  Fragments arranged by size: 

                1801     954     879     494     298     246      30
 
BsaI GGTCTCn'nnnn_

Cuts at:   4406    4406 
   Size:       4702
 
BsaAI yAC'GTr

Cuts at:    155    1692    2053     155 
   Size:       1537     361    2804

  Fragments arranged by size: 

                2804    1537     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2214    4253       6 
   Size:       2208    2039     455

  Fragments arranged by size: 

                2208    2039     455
 
BsaHI Gr'CG_yC

Cuts at:   1589    1637    2131    2379    3083    4579    1589 
   Size:         48     494     248     704    1496    1712

  Fragments arranged by size: 

                1712    1496     704     494     248      48
 
BsaWI w'CCGG_w

Cuts at:   1889    2410    3090    3364    3511    3922    1889 
   Size:        521     680     274     147     411    2669

  Fragments arranged by size: 

                2669     680     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    607     637     607 
   Size:         30    4672
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    388    1690     388 
   Size:       1302    3400
 
BseYI C'CCAG_C

Cuts at:    573     984    1305    1352    1433    1530    2125    2883    3462 
   Size:        411     321      47      81      97     595     758     579

Cuts at:   3462    3958     573 
   Size:        496    1317

  Fragments arranged by size: 

                1317     758     595     579     496     411     321      97
                  81      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    924     924 
   Size:       4702
 
BsiEI CG_ry'CG

Cuts at:    104    2242    2288    3498     104 
   Size:       2138      46    1210    1308

  Fragments arranged by size: 

                2138    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    380    1714    2492    2682    3476     380 
   Size:       1334     778     190     794    1606

  Fragments arranged by size: 

                1606    1334     794     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1282    1282 
   Size:       4702
 
Bsp1286I G_dGCh'C

Cuts at:    380    1041    1241    1714    2325    2418    2492    2682    2744 
   Size:        661     200     473     611      93      74     190      62

Cuts at:   2744    3476     380 
   Size:        732    1606

  Fragments arranged by size: 

                1606     732     661     611     473     200     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3922    3922 
   Size:       4702
 
BspHI T'CATG_A

Cuts at:    806    3878     806 
   Size:       3072    1630
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    327    1011    1750    1964    2266    2647     327 
   Size:        684     739     214     302     381    2382

  Fragments arranged by size: 

                2382     739     684     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2992    3046    4532    4697      50 
   Size:       2942      54    1486     165      55

  Fragments arranged by size: 

                2942    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    434    2612     434 
   Size:       2178    2524
 
BsrFI r'CCGG_y

Cuts at:    294    2698    2879    3090     294 
   Size:       2404     181     211    1906

  Fragments arranged by size: 

                2404    1906     211     181
 
BssSI C'ACGA_G

Cuts at:   2971    3331    2971 
   Size:        360    4342
 
BstAPI GCAn_nnn'nTGC

Cuts at:    732    2077     732 
   Size:       1345    3357
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4702
 
BstEII G'GTnAC_C

Cuts at:   1260    1260 
   Size:       4702
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1441    1441 
   Size:       4702
 
BstYI r'GATC_y

Cuts at:      1    1725    2192    2215    2550    2796    3810    3896    3908 
   Size:       1724     467      23     335     246    1014      86      12

Cuts at:   3908    4254       1 
   Size:        346     449

  Fragments arranged by size: 

                1724    1014     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    125     191     125 
   Size:         66    4636
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    162    2650    2831    2994     162 
   Size:       2488     181     163    1870

  Fragments arranged by size: 

                2488    1870     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1172    1943    1172 
   Size:        771    3931
 
DraI TTT'AAA

Cuts at:   1699    1699 
   Size:       4702
 
DraIII CAC_nnn'GTG

Cuts at:   1563    1563 
   Size:       4702
 
DrdI GACnn_nn'nnGTC

Cuts at:    862    2406    3266     862 
   Size:       1544     860    2298

  Fragments arranged by size: 

                2298    1544     860
 
EaeI y'GGCC_r

Cuts at:    204    1337    1682    2285    2459    2850    2877    4224     204 
   Size:       1133     345     603     174     391      27    1347     682

  Fragments arranged by size: 

                1347    1133     682     603     391     345     174      27
            
 
EagI C'GGCC_G

Cuts at:   2285    2285 
   Size:       4702
 
EarI CTCTTCn'nnn_

Cuts at:   2723    2933    2723 
   Size:        210    4492
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2908    3220    3366    4512    2908 
   Size:        312     146    1146    3098

  Fragments arranged by size: 

                3098    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:    378    1712     378 
   Size:       1334    3368
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     403     948    1027    1204    1315    1445    2525    2957 
   Size:        221     545      79     177     111     130    1080     432

Cuts at:   2957    3706    3974     182 
   Size:        749     268     910

  Fragments arranged by size: 

                1080     910     749     545     432     268     221     177
                 130     111      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:    709    1273     709 
   Size:        564    4138
 
EcoO109I rG'GnC_Cy

Cuts at:    183    1038    1204    1238    1839     183 
   Size:        855     166      34     601    3046

  Fragments arranged by size: 

                3046     855     601     166      34
 
EcoRI G'AATT_C

Cuts at:   1704    1704 
   Size:       4702
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2719    2719 
   Size:       4702
 
FspI TGC'GCA

Cuts at:   1660    2481    1660 
   Size:        821    3881
 
HaeII r_GCGC'y

Cuts at:   1316    1640    2134    2382    3406    4385    1316 
   Size:        324     494     248    1024     979    1633

  Fragments arranged by size: 

                1633    1024     979     494     324     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    607     639    2542    2572    2574    2604     607 
   Size:         32    1903      30       2      30    2705

  Fragments arranged by size: 

                2705    1903      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1739    1739 
   Size:       4702
 
KasI G'GCGC_C

Cuts at:   1636    2130    2378    1636 
   Size:        494     248    3960

  Fragments arranged by size: 

                3960     494     248
 
KpnI G_GTAC'C

Cuts at:    560    1720     560 
   Size:       1160    3542
 
MluI A'CGCG_T

Cuts at:   4135    4135 
   Size:       4702
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    748    3373    3557     748 
   Size:       2625     184    1893

  Fragments arranged by size: 

                2625    1893     184
 
MscI TGG'CCA

Cuts at:   1339    1684    2461    1339 
   Size:        345     777    3580

  Fragments arranged by size: 

                3580     777     345
 
MslI CAynn'nnrTG

Cuts at:    796    2689    2816     796 
   Size:       1893     127    2682

  Fragments arranged by size: 

                2682    1893     127
 
MspA1I CmG'CkG

Cuts at:    644    1811    2125    2485    3500    3745     644 
   Size:       1167     314     360    1015     245    1601

  Fragments arranged by size: 

                1601    1167    1015     360     314     245
 
NaeI GCC'GGC

Cuts at:   2881    2881 
   Size:       4702
 
NarI GG'CG_CC

Cuts at:   1637    2131    2379    1637 
   Size:        494     248    3960

  Fragments arranged by size: 

                3960     494     248
 
NcoI C'CATG_G

Cuts at:    125     191     125 
   Size:         66    4636
 
NgoMIV G'CCGG_C

Cuts at:   2879    2879 
   Size:       4702
 
NheI G'CTAG_C

Cuts at:   1476    1476 
   Size:       4702
 
NruI TCG'CGA

Cuts at:   3063    3063 
   Size:       4702
 
NspI r_CATG'y

Cuts at:    654    1753    2253    3162     654 
   Size:       1099     500     909    2194

  Fragments arranged by size: 

                2194    1099     909     500
 
PasI CC'CwG_GG

Cuts at:   1234    1234 
   Size:       4702
 
PciI A'CATG_T

Cuts at:   3158    3158 
   Size:       4702
 
PfoI T'CCnGG_A

Cuts at:    380     380 
   Size:       4702
 
PmeI GTTT'AAAC

Cuts at:   1699    1699 
   Size:       4702
 
PmlI CAC'GTG

Cuts at:    155     155 
   Size:       4702
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    943     975    3841    3873     943 
   Size:         32    2866      32    1772

  Fragments arranged by size: 

                2866    1772      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1204    1204 
   Size:       4702
 
PspOMI G'GGCC_C

Cuts at:   1037    1237    1037 
   Size:        200    4502
 
PspXI vC'TCGA_Gb

Cuts at:   1330    1330 
   Size:       4702
 
PstI C_TGCA'G

Cuts at:    623    1022    1055    1286    1572    1747    2432     623 
   Size:        399      33     231     286     175     685    2893

  Fragments arranged by size: 

                2893     685     399     286     231     175      33
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4702
 
PvuII CAG'CTG

Cuts at:   2125    2485    2125 
   Size:        360    4342
 
RsrII CG'GwC_CG

Cuts at:   2895    2895 
   Size:       4702
 
SacI G_AGCT'C

Cuts at:    380    1714     380 
   Size:       1334    3368
 
SalI G'TCGA_C

Cuts at:   1737    1737 
   Size:       4702
 
SanDI GG'GwC_CC

Cuts at:   1204    1204 
   Size:       4702
 
SapI GCTCTTCn'nnn_

Cuts at:   2723    2933    2723 
   Size:        210    4492
 
SbfI CC_TGCA'GG

Cuts at:   1022    1747    1022 
   Size:        725    3977
 
ScaI AGT'ACT

Cuts at:   1198    4219    1198 
   Size:       3021    1681
 
SexAI A'CCwGG_T

Cuts at:    137    1028     137 
   Size:        891    3811
 
SfcI C'TryA_G

Cuts at:     33     619    1018    1051    1282    1568    1743    2428    3423 
   Size:        586     399      33     231     286     175     685     995

Cuts at:   3423    3614      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     995     685     586     399     286     231     191
                 175      33
 
SfoI GGC'GCC

Cuts at:   1638    2132    2380    1638 
   Size:        494     248    3960

  Fragments arranged by size: 

                3960     494     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4702
 
SmaI CCC'GGG

Cuts at:   1722    1722 
   Size:       4702
 
SmlI C'TyrA_G

Cuts at:    661    1330    1615    1645    1855    2770    3264    3526     661 
   Size:        669     285      30     210     915     494     262    1837

  Fragments arranged by size: 

                1837     915     669     494     285     262     210      30
            
 
SnaBI TAC'GTA

Cuts at:   1692    1692 
   Size:       4702
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4702
 
SphI G_CATG'C

Cuts at:    654    1753    2253     654 
   Size:       1099     500    3103

  Fragments arranged by size: 

                3103    1099     500
 
StuI AGG'CCT

Cuts at:    607    1004     607 
   Size:        397    4305
 
StyI C'CwwG_G

Cuts at:    125     191     602     611    1834    2188     125 
   Size:         66     411       9    1223     354    2639

  Fragments arranged by size: 

                2639    1223     411     354      66       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    276     534    1344    2283    2416    2875    3086     276 
   Size:        258     810     939     133     459     211    1892

  Fragments arranged by size: 

                1892     939     810     459     258     211     133
 
TatI w'GTAC_w

Cuts at:    665    1196    4217     665 
   Size:        531    3021    1150

  Fragments arranged by size: 

                3021    1150     531
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1018    1050    4219    4251    1018 
   Size:         32    3169      32    1469

  Fragments arranged by size: 

                3169    1469      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2497    2497 
   Size:       4702
 
XbaI T'CTAG_A

Cuts at:   1731    1731 
   Size:       4702
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1179    1601    2195    1179 
   Size:        422     594    3686

  Fragments arranged by size: 

                3686     594     422
 
XhoI C'TCGA_G

Cuts at:   1330    1330 
   Size:       4702
 
XmaI C'CCGG_G

Cuts at:   1720    1720 
   Size:       4702
 
XmnI GAAnn'nnTTC

Cuts at:   1144    1144 
   Size:       4702
 
ZraI GAC'GTC

Cuts at:   1590    1590 
   Size:       4702
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NruI     NspI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AloI     AscI    AvrII     BaeI    BfrBI
    BsiWI     BsmI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NotI
     NsiI     PacI    PflMI    PshAI     PsiI     PsrI    SacII     SfiI
    SgrAI     SrfI     SspI     SwaI
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