Restriction map for
pF1KSDA0948
(Circular) (Six-base) MAPSORT of: pF1KSDA0948.seq Check: 3228 from: 1 to: 4702
pF1KSDA0948 4702 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 327 1011 327
Size: 684 4018
AatII G_ACGT'C
Cuts at: 1592 1592
Size: 4702
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4702
Acc65I G'GTAC_C
Cuts at: 556 1716 556
Size: 1160 3542
AclI AA'CG_TT
Cuts at: 4542 4542
Size: 4702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1315 2525 2957 3706 3974 948
Size: 367 1210 432 749 268 1676
Fragments arranged by size:
1676 1210 749 432 367 268
AflIII A'CryG_T
Cuts at: 2052 3158 4135 2052
Size: 1106 977 2619
Fragments arranged by size:
2619 1106 977
AgeI A'CCGG_T
Cuts at: 3090 3090
Size: 4702
AleI CACnn'nnGTG
Cuts at: 796 796
Size: 4702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1543 1543
Size: 4702
AlwNI CAG_nnn'CTG
Cuts at: 732 1155 2005 3574 732
Size: 423 850 1569 1860
Fragments arranged by size:
1860 1569 850 423
ApaI G_GGCC'C
Cuts at: 1041 1241 1041
Size: 200 4502
ApaLI G'TGCA_C
Cuts at: 3472 3472
Size: 4702
ApoI r'AATT_y
Cuts at: 1096 1704 4055 4311 1096
Size: 608 2351 256 1487
Fragments arranged by size:
2351 1487 608 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4702
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4702
AvaI C'yCGr_G
Cuts at: 1192 1200 1330 1720 1192
Size: 8 130 390 4174
Fragments arranged by size:
4174 390 130 8
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4702
BanI G'GyrC_C
Cuts at: 556 1419 1636 1716 2130 2378 2413 556
Size: 863 217 80 414 248 35 2845
Fragments arranged by size:
2845 863 414 248 217 80 35
BanII G_rGCy'C
Cuts at: 380 1041 1241 1714 2744 380
Size: 661 200 473 1030 2338
Fragments arranged by size:
2338 1030 661 473 200
BbeI G_GCGC'C
Cuts at: 1640 2134 2382 1640
Size: 494 248 3960
Fragments arranged by size:
3960 494 248
BbsI GAAGACnn'nnnn_
Cuts at: 860 860
Size: 4702
BbvCI CC'TCA_GC
Cuts at: 249 1242 1367 1673 249
Size: 993 125 306 3278
Fragments arranged by size:
3278 993 306 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 137 171 137
Size: 34 4668
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3361 4702 2598
Size: 763 1341 2598
Fragments arranged by size:
2598 1341 763
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4702
BglI GCCn_nnn'nGGC
Cuts at: 685 685
Size: 4702
BglII A'GATC_T
Cuts at: 2215 4254 2215
Size: 2039 2663
BlpI GC'TnA_GC
Cuts at: 740 1812 740
Size: 1072 3630
Bme1580I G_kGCm'C
Cuts at: 1041 1241 2325 2418 3476 1041
Size: 200 1084 93 1058 2267
Fragments arranged by size:
2267 1084 1058 200 93
BmgBI CAC'GTC
Cuts at: 864 864
Size: 4702
BmrI ACTGGGnnnn_n'
Cuts at: 1451 1912 1993 2328 4474 1451
Size: 461 81 335 2146 1679
Fragments arranged by size:
2146 1679 461 335 81
BmtI G_CTAG'C
Cuts at: 1480 1480
Size: 4702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 3067 738
Size: 2329 2373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 403 1027 1204 1445 182
Size: 221 624 177 241 3439
Fragments arranged by size:
3439 624 241 221 177
Bpu10I CC'TnA_GC
Cuts at: 249 1242 1367 1673 249
Size: 993 125 306 3278
Fragments arranged by size:
3278 993 306 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1600 1630 1876 2755 3249 3547 646
Size: 954 30 246 879 494 298 1801
Fragments arranged by size:
1801 954 879 494 298 246 30
BsaI GGTCTCn'nnnn_
Cuts at: 4406 4406
Size: 4702
BsaAI yAC'GTr
Cuts at: 155 1692 2053 155
Size: 1537 361 2804
Fragments arranged by size:
2804 1537 361
BsaBI GATnn'nnATC
Cuts at: 6 2214 4253 6
Size: 2208 2039 455
Fragments arranged by size:
2208 2039 455
BsaHI Gr'CG_yC
Cuts at: 1589 1637 2131 2379 3083 4579 1589
Size: 48 494 248 704 1496 1712
Fragments arranged by size:
1712 1496 704 494 248 48
BsaWI w'CCGG_w
Cuts at: 1889 2410 3090 3364 3511 3922 1889
Size: 521 680 274 147 411 2669
Fragments arranged by size:
2669 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 607
Size: 30 4672
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 1690 388
Size: 1302 3400
BseYI C'CCAG_C
Cuts at: 573 984 1305 1352 1433 1530 2125 2883 3462
Size: 411 321 47 81 97 595 758 579
Cuts at: 3462 3958 573
Size: 496 1317
Fragments arranged by size:
1317 758 595 579 496 411 321 97
81 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 924
Size: 4702
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3498 104
Size: 2138 46 1210 1308
Fragments arranged by size:
2138 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 380 1714 2492 2682 3476 380
Size: 1334 778 190 794 1606
Fragments arranged by size:
1606 1334 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1282 1282
Size: 4702
Bsp1286I G_dGCh'C
Cuts at: 380 1041 1241 1714 2325 2418 2492 2682 2744
Size: 661 200 473 611 93 74 190 62
Cuts at: 2744 3476 380
Size: 732 1606
Fragments arranged by size:
1606 732 661 611 473 200 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3922 3922
Size: 4702
BspHI T'CATG_A
Cuts at: 806 3878 806
Size: 3072 1630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 1011 1750 1964 2266 2647 327
Size: 684 739 214 302 381 2382
Fragments arranged by size:
2382 739 684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4532 4697 50
Size: 2942 54 1486 165 55
Fragments arranged by size:
2942 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 434 2612 434
Size: 2178 2524
BsrFI r'CCGG_y
Cuts at: 294 2698 2879 3090 294
Size: 2404 181 211 1906
Fragments arranged by size:
2404 1906 211 181
BssSI C'ACGA_G
Cuts at: 2971 3331 2971
Size: 360 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 732 2077 732
Size: 1345 3357
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4702
BstEII G'GTnAC_C
Cuts at: 1260 1260
Size: 4702
BstXI CCAn_nnnn'nTGG
Cuts at: 1441 1441
Size: 4702
BstYI r'GATC_y
Cuts at: 1 1725 2192 2215 2550 2796 3810 3896 3908
Size: 1724 467 23 335 246 1014 86 12
Cuts at: 3908 4254 1
Size: 346 449
Fragments arranged by size:
1724 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 191 125
Size: 66 4636
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 162 2650 2831 2994 162
Size: 2488 181 163 1870
Fragments arranged by size:
2488 1870 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1943 1172
Size: 771 3931
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4702
DraIII CAC_nnn'GTG
Cuts at: 1563 1563
Size: 4702
DrdI GACnn_nn'nnGTC
Cuts at: 862 2406 3266 862
Size: 1544 860 2298
Fragments arranged by size:
2298 1544 860
EaeI y'GGCC_r
Cuts at: 204 1337 1682 2285 2459 2850 2877 4224 204
Size: 1133 345 603 174 391 27 1347 682
Fragments arranged by size:
1347 1133 682 603 391 345 174 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4702
EarI CTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3220 3366 4512 2908
Size: 312 146 1146 3098
Fragments arranged by size:
3098 1146 312 146
EcoICRI GAG'CTC
Cuts at: 378 1712 378
Size: 1334 3368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 403 948 1027 1204 1315 1445 2525 2957
Size: 221 545 79 177 111 130 1080 432
Cuts at: 2957 3706 3974 182
Size: 749 268 910
Fragments arranged by size:
1080 910 749 545 432 268 221 177
130 111 79
EcoNI CCTnn'n_nnAGG
Cuts at: 709 1273 709
Size: 564 4138
EcoO109I rG'GnC_Cy
Cuts at: 183 1038 1204 1238 1839 183
Size: 855 166 34 601 3046
Fragments arranged by size:
3046 855 601 166 34
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4702
FspI TGC'GCA
Cuts at: 1660 2481 1660
Size: 821 3881
HaeII r_GCGC'y
Cuts at: 1316 1640 2134 2382 3406 4385 1316
Size: 324 494 248 1024 979 1633
Fragments arranged by size:
1633 1024 979 494 324 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 607 639 2542 2572 2574 2604 607
Size: 32 1903 30 2 30 2705
Fragments arranged by size:
2705 1903 32 30 30 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4702
KasI G'GCGC_C
Cuts at: 1636 2130 2378 1636
Size: 494 248 3960
Fragments arranged by size:
3960 494 248
KpnI G_GTAC'C
Cuts at: 560 1720 560
Size: 1160 3542
MluI A'CGCG_T
Cuts at: 4135 4135
Size: 4702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 748 3373 3557 748
Size: 2625 184 1893
Fragments arranged by size:
2625 1893 184
MscI TGG'CCA
Cuts at: 1339 1684 2461 1339
Size: 345 777 3580
Fragments arranged by size:
3580 777 345
MslI CAynn'nnrTG
Cuts at: 796 2689 2816 796
Size: 1893 127 2682
Fragments arranged by size:
2682 1893 127
MspA1I CmG'CkG
Cuts at: 644 1811 2125 2485 3500 3745 644
Size: 1167 314 360 1015 245 1601
Fragments arranged by size:
1601 1167 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4702
NarI GG'CG_CC
Cuts at: 1637 2131 2379 1637
Size: 494 248 3960
Fragments arranged by size:
3960 494 248
NcoI C'CATG_G
Cuts at: 125 191 125
Size: 66 4636
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4702
NheI G'CTAG_C
Cuts at: 1476 1476
Size: 4702
NruI TCG'CGA
Cuts at: 3063 3063
Size: 4702
NspI r_CATG'y
Cuts at: 654 1753 2253 3162 654
Size: 1099 500 909 2194
Fragments arranged by size:
2194 1099 909 500
PasI CC'CwG_GG
Cuts at: 1234 1234
Size: 4702
PciI A'CATG_T
Cuts at: 3158 3158
Size: 4702
PfoI T'CCnGG_A
Cuts at: 380 380
Size: 4702
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4702
PmlI CAC'GTG
Cuts at: 155 155
Size: 4702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 943 975 3841 3873 943
Size: 32 2866 32 1772
Fragments arranged by size:
2866 1772 32 32
PpuMI rG'GwC_Cy
Cuts at: 1204 1204
Size: 4702
PspOMI G'GGCC_C
Cuts at: 1037 1237 1037
Size: 200 4502
PspXI vC'TCGA_Gb
Cuts at: 1330 1330
Size: 4702
PstI C_TGCA'G
Cuts at: 623 1022 1055 1286 1572 1747 2432 623
Size: 399 33 231 286 175 685 2893
Fragments arranged by size:
2893 685 399 286 231 175 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4702
PvuII CAG'CTG
Cuts at: 2125 2485 2125
Size: 360 4342
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4702
SacI G_AGCT'C
Cuts at: 380 1714 380
Size: 1334 3368
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4702
SanDI GG'GwC_CC
Cuts at: 1204 1204
Size: 4702
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4492
SbfI CC_TGCA'GG
Cuts at: 1022 1747 1022
Size: 725 3977
ScaI AGT'ACT
Cuts at: 1198 4219 1198
Size: 3021 1681
SexAI A'CCwGG_T
Cuts at: 137 1028 137
Size: 891 3811
SfcI C'TryA_G
Cuts at: 33 619 1018 1051 1282 1568 1743 2428 3423
Size: 586 399 33 231 286 175 685 995
Cuts at: 3423 3614 33
Size: 191 1121
Fragments arranged by size:
1121 995 685 586 399 286 231 191
175 33
SfoI GGC'GCC
Cuts at: 1638 2132 2380 1638
Size: 494 248 3960
Fragments arranged by size:
3960 494 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4702
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4702
SmlI C'TyrA_G
Cuts at: 661 1330 1615 1645 1855 2770 3264 3526 661
Size: 669 285 30 210 915 494 262 1837
Fragments arranged by size:
1837 915 669 494 285 262 210 30
SnaBI TAC'GTA
Cuts at: 1692 1692
Size: 4702
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4702
SphI G_CATG'C
Cuts at: 654 1753 2253 654
Size: 1099 500 3103
Fragments arranged by size:
3103 1099 500
StuI AGG'CCT
Cuts at: 607 1004 607
Size: 397 4305
StyI C'CwwG_G
Cuts at: 125 191 602 611 1834 2188 125
Size: 66 411 9 1223 354 2639
Fragments arranged by size:
2639 1223 411 354 66 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 534 1344 2283 2416 2875 3086 276
Size: 258 810 939 133 459 211 1892
Fragments arranged by size:
1892 939 810 459 258 211 133
TatI w'GTAC_w
Cuts at: 665 1196 4217 665
Size: 531 3021 1150
Fragments arranged by size:
3021 1150 531
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1018 1050 4219 4251 1018
Size: 32 3169 32 1469
Fragments arranged by size:
3169 1469 32 32
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4702
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 1601 2195 1179
Size: 422 594 3686
Fragments arranged by size:
3686 594 422
XhoI C'TCGA_G
Cuts at: 1330 1330
Size: 4702
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4702
XmnI GAAnn'nnTTC
Cuts at: 1144 1144
Size: 4702
ZraI GAC'GTC
Cuts at: 1590 1590
Size: 4702
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BfrBI
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PflMI PshAI PsiI PsrI SacII SfiI
SgrAI SrfI SspI SwaI