Restriction map for
pF1KSDA0955
(Circular) (Six-base) MAPSORT of: pF1KSDA0955.seq Check: 8393 from: 1 to: 4432
pF1KSDA0955 4432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 596 596
Size: 4432
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4432
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4432
AclI AA'CG_TT
Cuts at: 4272 4272
Size: 4432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1344 2255 2687 3436 3704 323
Size: 1021 911 432 749 268 1051
Fragments arranged by size:
1051 1021 911 749 432 268
AflIII A'CryG_T
Cuts at: 1782 2888 3865 1782
Size: 1106 977 2349
Fragments arranged by size:
2349 1106 977
AgeI A'CCGG_T
Cuts at: 2820 2820
Size: 4432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 904 936 904
Size: 32 4400
AlwNI CAG_nnn'CTG
Cuts at: 294 462 495 1735 3304 294
Size: 168 33 1240 1569 1422
Fragments arranged by size:
1569 1422 1240 168 33
ApaLI G'TGCA_C
Cuts at: 3202 3202
Size: 4432
ApoI r'AATT_y
Cuts at: 1001 1018 1413 1434 3785 4041 1001
Size: 17 395 21 2351 256 1392
Fragments arranged by size:
2351 1392 395 256 21 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4432
AvaI C'yCGr_G
Cuts at: 653 1450 653
Size: 797 3635
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1085 1118 1085
Size: 33 4399
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4432
BanI G'GyrC_C
Cuts at: 1446 1860 2108 2143 1446
Size: 414 248 35 3735
Fragments arranged by size:
3735 414 248 35
BanII G_rGCy'C
Cuts at: 994 1444 2474 994
Size: 450 1030 2952
Fragments arranged by size:
2952 1030 450
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4184
BciVI GTATCCnnnnn_n'
Cuts at: 1086 2328 3091 4432 1086
Size: 1242 763 1341 1086
Fragments arranged by size:
1341 1242 1086 763
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4432
BglII A'GATC_T
Cuts at: 1945 3984 1945
Size: 2039 2393
BlpI GC'TnA_GC
Cuts at: 1315 1542 1315
Size: 227 4205
Bme1580I G_kGCm'C
Cuts at: 2055 2148 3206 2055
Size: 93 1058 3281
Fragments arranged by size:
3281 1058 93
BmgBI CAC'GTC
Cuts at: 1353 1353
Size: 4432
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4204 1642
Size: 81 335 2146 1870
Fragments arranged by size:
2146 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 661 1016 1102 1216 471
Size: 190 355 86 114 3687
Fragments arranged by size:
3687 355 190 114 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1606 2485 2979 3277 1075
Size: 531 879 494 298 2230
Fragments arranged by size:
2230 879 531 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4136 4136
Size: 4432
BsaAI yAC'GTr
Cuts at: 1422 1783 1422
Size: 361 4071
BsaBI GATnn'nnATC
Cuts at: 6 706 1944 3983 6
Size: 700 1238 2039 455
Fragments arranged by size:
2039 1238 700 455
BsaHI Gr'CG_yC
Cuts at: 593 827 1861 2109 2813 4309 593
Size: 234 1034 248 704 1496 716
Fragments arranged by size:
1496 1034 716 704 248 234
BsaWI w'CCGG_w
Cuts at: 1619 2140 2820 3094 3241 3652 1619
Size: 521 680 274 147 411 2399
Fragments arranged by size:
2399 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 556 586 556
Size: 30 4402
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 1133 550
Size: 583 3849
BseYI C'CCAG_C
Cuts at: 688 820 1027 1855 2613 3192 3688 688
Size: 132 207 828 758 579 496 1432
Fragments arranged by size:
1432 828 758 579 496 207 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1178
Size: 4432
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3228 104
Size: 1868 46 1210 1308
Fragments arranged by size:
1868 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 340 1223 1358 1444 2222 2412 3206 340
Size: 883 135 86 778 190 794 1566
Fragments arranged by size:
1566 883 794 778 190 135 86
BsmBI CGTCTCn'nnnn_
Cuts at: 600 600
Size: 4432
Bsp1286I G_dGCh'C
Cuts at: 340 994 1223 1358 1444 2055 2148 2222 2412
Size: 654 229 135 86 611 93 74 190
Cuts at: 2412 2474 3206 340
Size: 62 732 1566
Fragments arranged by size:
1566 732 654 611 229 190 135 93
86 74 62
BspEI T'CCGG_A
Cuts at: 3652 3652
Size: 4432
BspHI T'CATG_A
Cuts at: 3608 3608
Size: 4432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 1480 1694 1996 2377 482
Size: 998 214 302 381 2537
Fragments arranged by size:
2537 998 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2722 2776 4262 4427 50
Size: 2672 54 1486 165 55
Fragments arranged by size:
2672 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4432
BsrFI r'CCGG_y
Cuts at: 1181 2428 2609 2820 1181
Size: 1247 181 211 2793
Fragments arranged by size:
2793 1247 211 181
BssSI C'ACGA_G
Cuts at: 1390 2701 3061 1390
Size: 1311 360 2761
Fragments arranged by size:
2761 1311 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4432
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4432
BstYI r'GATC_y
Cuts at: 1 1113 1455 1922 1945 2280 2526 3540 3626
Size: 1112 342 467 23 335 246 1014 86
Cuts at: 3626 3638 3984 1
Size: 12 346 449
Fragments arranged by size:
1112 1014 467 449 346 342 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 870 870
Size: 4432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4088
Fragments arranged by size:
4088 181 163
BtsI GCAGTG_nn'
Cuts at: 365 521 1673 365
Size: 156 1152 3124
Fragments arranged by size:
3124 1152 156
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4432
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2996 2136
Size: 860 3572
EaeI y'GGCC_r
Cuts at: 2015 2189 2580 2607 3954 2015
Size: 174 391 27 1347 2493
Fragments arranged by size:
2493 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4432
EarI CTCTTCn'nnn_
Cuts at: 212 1363 2453 2663 212
Size: 1151 1090 210 1981
Fragments arranged by size:
1981 1151 1090 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2950 3096 4242 2638
Size: 312 146 1146 2828
Fragments arranged by size:
2828 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 471 661 1016 1102 1216 1344 2255 2687
Size: 148 190 355 86 114 128 911 432
Cuts at: 2687 3436 3704 323
Size: 749 268 1051
Fragments arranged by size:
1051 911 749 432 355 268 190 148
128 114 86
EcoNI CCTnn'n_nnAGG
Cuts at: 1151 1151
Size: 4432
EcoO109I rG'GnC_Cy
Cuts at: 298 1206 1338 1380 1569 298
Size: 908 132 42 189 3161
Fragments arranged by size:
3161 908 189 132 42
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 822 1400 2449 822
Size: 578 1049 2805
Fragments arranged by size:
2805 1049 578
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4432
HaeII r_GCGC'y
Cuts at: 1864 2112 3136 4115 1864
Size: 248 1024 979 2181
Fragments arranged by size:
2181 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 759 791 2272 2302 2304 2334 196
Size: 32 531 32 1481 30 2 30 2294
Fragments arranged by size:
2294 1481 531 32 32 30 30 2
HincII GTy'rAC
Cuts at: 177 1469 177
Size: 1292 3140
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4184
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4432
MluI A'CGCG_T
Cuts at: 3865 3865
Size: 4432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 782 3103 3287 782
Size: 2321 184 1927
Fragments arranged by size:
2321 1927 184
MscI TGG'CCA
Cuts at: 2191 2191
Size: 4432
MslI CAynn'nnrTG
Cuts at: 2419 2546 2419
Size: 127 4305
MspA1I CmG'CkG
Cuts at: 1541 1855 2215 3230 3475 1541
Size: 314 360 1015 245 2498
Fragments arranged by size:
2498 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4432
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4184
NcoI C'CATG_G
Cuts at: 870 870
Size: 4432
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4432
NruI TCG'CGA
Cuts at: 2793 2793
Size: 4432
NspI r_CATG'y
Cuts at: 1483 1983 2892 1483
Size: 500 909 3023
Fragments arranged by size:
3023 909 500
PciI A'CATG_T
Cuts at: 2888 2888
Size: 4432
PfoI T'CCnGG_A
Cuts at: 448 638 677 1232 448
Size: 190 39 555 3648
Fragments arranged by size:
3648 555 190 39
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3571 3603 3571
Size: 32 4400
PpuMI rG'GwC_Cy
Cuts at: 1206 1338 1380 1206
Size: 132 42 4258
Fragments arranged by size:
4258 132 42
PsiI TTA'TAA
Cuts at: 278 278
Size: 4432
PstI C_TGCA'G
Cuts at: 479 528 887 1477 2162 479
Size: 49 359 590 685 2749
Fragments arranged by size:
2749 685 590 359 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4432
PvuII CAG'CTG
Cuts at: 1855 2215 1855
Size: 360 4072
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4432
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4432
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4432
SanDI GG'GwC_CC
Cuts at: 1380 1380
Size: 4432
SapI GCTCTTCn'nnn_
Cuts at: 1363 2453 2663 1363
Size: 1090 210 3132
Fragments arranged by size:
3132 1090 210
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4432
ScaI AGT'ACT
Cuts at: 3949 3949
Size: 4432
SfcI C'TryA_G
Cuts at: 33 475 524 883 984 1473 2158 3153 3344
Size: 442 49 359 101 489 685 995 191
Cuts at: 3344 33
Size: 1121
Fragments arranged by size:
1121 995 685 489 442 359 191 101
49
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4432
SmaI CCC'GGG
Cuts at: 655 1452 655
Size: 797 3635
SmlI C'TyrA_G
Cuts at: 1054 1585 2500 2994 3256 1054
Size: 531 915 494 262 2230
Fragments arranged by size:
2230 915 531 494 262
SnaBI TAC'GTA
Cuts at: 1422 1422
Size: 4432
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4432
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3932
StuI AGG'CCT
Cuts at: 398 1309 398
Size: 911 3521
StyI C'CwwG_G
Cuts at: 579 870 965 1564 1918 579
Size: 291 95 599 354 3093
Fragments arranged by size:
3093 599 354 291 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2013 2146 2605 2816 2013
Size: 133 459 211 3629
Fragments arranged by size:
3629 459 211 133
TatI w'GTAC_w
Cuts at: 3947 3947
Size: 4432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 556 588 711 743 3949 3981 556
Size: 32 123 32 3206 32 1007
Fragments arranged by size:
3206 1007 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4432
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4432
XmaI C'CCGG_G
Cuts at: 653 1450 653
Size: 797 3635
XmnI GAAnn'nnTTC
Cuts at: 1046 1046
Size: 4432
ZraI GAC'GTC
Cuts at: 594 594
Size: 4432
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PmlI
PshAI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SrfI SspI SwaI XhoI