Restriction map for
pF1KSDA0957
(Circular) (Six-base) MAPSORT of: pF1KSDA0957.seq Check: 2241 from: 1 to: 5215
pF1KSDA0957 5215 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2251 2251
Size: 5215
Acc65I G'GTAC_C
Cuts at: 2229 2229
Size: 5215
AclI AA'CG_TT
Cuts at: 5055 5055
Size: 5215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 3038 3470 4219 4487 331
Size: 2707 432 749 268 1059
Fragments arranged by size:
2707 1059 749 432 268
AflIII A'CryG_T
Cuts at: 2565 3671 4648 2565
Size: 1106 977 3132
Fragments arranged by size:
3132 1106 977
AgeI A'CCGG_T
Cuts at: 3603 3603
Size: 5215
AhdI GACnn_n'nnGTC
Cuts at: 1658 1658
Size: 5215
AleI CACnn'nnGTG
Cuts at: 376 376
Size: 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 287 683 287
Size: 396 4819
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 5183
AlwNI CAG_nnn'CTG
Cuts at: 1557 1752 1943 2518 4087 1557
Size: 195 191 575 1569 2685
Fragments arranged by size:
2685 1569 575 195 191
ApaI G_GGCC'C
Cuts at: 1856 1856
Size: 5215
ApaLI G'TGCA_C
Cuts at: 3985 3985
Size: 5215
ApoI r'AATT_y
Cuts at: 2217 4568 4824 2217
Size: 2351 256 2608
Fragments arranged by size:
2608 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5215
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5215
AvaI C'yCGr_G
Cuts at: 2233 2233
Size: 5215
BamHI G'GATC_C
Cuts at: 2238 2238
Size: 5215
BanI G'GyrC_C
Cuts at: 223 1239 2229 2643 2891 2926 223
Size: 1016 990 414 248 35 2512
Fragments arranged by size:
2512 1016 990 414 248 35
BanII G_rGCy'C
Cuts at: 1856 2227 3257 1856
Size: 371 1030 3814
Fragments arranged by size:
3814 1030 371
BbeI G_GCGC'C
Cuts at: 227 2647 2895 227
Size: 2420 248 2547
Fragments arranged by size:
2547 2420 248
BbsI GAAGACnn'nnnn_
Cuts at: 1751 1751
Size: 5215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 437 403
Size: 34 5181
BciVI GTATCCnnnnn_n'
Cuts at: 3111 3874 5215 3111
Size: 763 1341 3111
Fragments arranged by size:
3111 1341 763
BclI T'GATC_A
Cuts at: 1839 2733 1839
Size: 894 4321
BglI GCCn_nnn'nGGC
Cuts at: 1908 1908
Size: 5215
BglII A'GATC_T
Cuts at: 302 1834 2728 4767 302
Size: 1532 894 2039 750
Fragments arranged by size:
2039 1532 894 750
BlpI GC'TnA_GC
Cuts at: 2325 2325
Size: 5215
Bme1580I G_kGCm'C
Cuts at: 426 1856 1920 2838 2931 3989 426
Size: 1430 64 918 93 1058 1652
Fragments arranged by size:
1652 1430 1058 918 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 2019 2425 2506 2841 4987 2019
Size: 406 81 335 2146 2247
Fragments arranged by size:
2247 2146 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 877 1303 1579 1595 1673 1726 2035 764
Size: 113 426 276 16 78 53 309 3944
Fragments arranged by size:
3944 426 309 276 113 78 53 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 3268 3762 4060 2389
Size: 879 494 298 3544
Fragments arranged by size:
3544 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4919 4919
Size: 5215
BsaAI yAC'GTr
Cuts at: 2205 2566 2205
Size: 361 4854
BsaBI GATnn'nnATC
Cuts at: 6 2727 4766 6
Size: 2721 2039 455
Fragments arranged by size:
2721 2039 455
BsaHI Gr'CG_yC
Cuts at: 224 2644 2892 3596 5092 224
Size: 2420 248 704 1496 347
Fragments arranged by size:
2420 1496 704 347 248
BsaWI w'CCGG_w
Cuts at: 2402 2923 3603 3877 4024 4435 2402
Size: 521 680 274 147 411 3182
Fragments arranged by size:
3182 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 568 598 894 924 1276 1306 1741
Size: 30 29 30 296 30 352 30 435
Cuts at: 1741 1771 509
Size: 30 3953
Fragments arranged by size:
3953 435 352 296 30 30 30 30
30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 694 1047 1324 691
Size: 3 353 277 4582
Fragments arranged by size:
4582 353 277 3
BseYI C'CCAG_C
Cuts at: 943 1326 1359 1382 1849 1919 2114 2638 3396
Size: 383 33 23 467 70 195 524 758
Cuts at: 3396 3975 4471 943
Size: 579 496 1687
Fragments arranged by size:
1687 758 579 524 496 467 383 195
70 33 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 544 2209 134
Size: 410 1665 3140
Fragments arranged by size:
3140 1665 410
BsiEI CG_ry'CG
Cuts at: 104 146 2755 2801 4011 104
Size: 42 2609 46 1210 1308
Fragments arranged by size:
2609 1308 1210 46 42
BsiHKAI G_wGCw'C
Cuts at: 595 2227 3005 3195 3989 595
Size: 1632 778 190 794 1821
Fragments arranged by size:
1821 1632 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 426 595 1856 1920 2227 2838 2931 3005 3195
Size: 169 1261 64 307 611 93 74 190
Cuts at: 3195 3257 3989 426
Size: 62 732 1652
Fragments arranged by size:
1652 1261 732 611 307 190 169 93
74 64 62
BspEI T'CCGG_A
Cuts at: 4435 4435
Size: 5215
BspHI T'CATG_A
Cuts at: 4391 4391
Size: 5215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1121 1648 2263 2477 2779 3160 1121
Size: 527 615 214 302 381 3176
Fragments arranged by size:
3176 615 527 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3505 3559 5045 5210 50
Size: 3455 54 1486 165 55
Fragments arranged by size:
3455 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3125 3125
Size: 5215
BsrFI r'CCGG_y
Cuts at: 3211 3392 3603 3211
Size: 181 211 4823
Fragments arranged by size:
4823 211 181
BsrGI T'GTAC_A
Cuts at: 1768 1768
Size: 5215
BssHII G'CGCG_C
Cuts at: 670 670
Size: 5215
BssSI C'ACGA_G
Cuts at: 3484 3844 3484
Size: 360 4855
BstAPI GCAn_nnn'nTGC
Cuts at: 1392 2590 1392
Size: 1198 4017
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5215
BstEII G'GTnAC_C
Cuts at: 237 237
Size: 5215
BstXI CCAn_nnnn'nTGG
Cuts at: 343 378 1096 343
Size: 35 718 4462
Fragments arranged by size:
4462 718 35
BstYI r'GATC_y
Cuts at: 1 302 1834 2238 2705 2728 3063 3309 4323
Size: 301 1532 404 467 23 335 246 1014
Cuts at: 4323 4409 4421 4767 1
Size: 86 12 346 449
Fragments arranged by size:
1532 1014 467 449 404 346 335 301
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 381 3163 3344 3507 381
Size: 2782 181 163 2089
Fragments arranged by size:
2782 2089 181 163
BtsI GCAGTG_nn'
Cuts at: 707 927 954 1724 2456 707
Size: 220 27 770 732 3466
Fragments arranged by size:
3466 770 732 220 27
DraI TTT'AAA
Cuts at: 2212 2212
Size: 5215
DrdI GACnn_nn'nnGTC
Cuts at: 331 2919 3779 331
Size: 2588 860 1767
Fragments arranged by size:
2588 1767 860
EaeI y'GGCC_r
Cuts at: 249 611 791 962 2798 2972 3363 3390 4737
Size: 362 180 171 1836 174 391 27 1347
Cuts at: 4737 249
Size: 727
Fragments arranged by size:
1836 1347 727 391 362 180 174 171
27
EagI C'GGCC_G
Cuts at: 2798 2798
Size: 5215
EarI CTCTTCn'nnn_
Cuts at: 899 980 1888 2153 3236 3446 899
Size: 81 908 265 1083 210 2668
Fragments arranged by size:
2668 1083 908 265 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3421 3733 3879 5025 3421
Size: 312 146 1146 3611
Fragments arranged by size:
3611 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2225 2225
Size: 5215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 764 877 1303 1579 1595 1673 1726 2035
Size: 433 113 426 276 16 78 53 309
Cuts at: 2035 3038 3470 4219 4487 331
Size: 1003 432 749 268 1059
Fragments arranged by size:
1059 1003 749 433 432 426 309 276
268 113 78 53 16
EcoO109I rG'GnC_Cy
Cuts at: 617 1586 1713 1853 2352 617
Size: 969 127 140 499 3480
Fragments arranged by size:
3480 969 499 140 127
EcoRI G'AATT_C
Cuts at: 2217 2217
Size: 5215
EcoRV GAT'ATC
Cuts at: 1508 1508
Size: 5215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 910 2182 3232 910
Size: 1272 1050 2893
Fragments arranged by size:
2893 1272 1050
FspI TGC'GCA
Cuts at: 2994 2994
Size: 5215
HaeII r_GCGC'y
Cuts at: 165 227 406 2647 2895 3919 4898 165
Size: 62 179 2241 248 1024 979 482
Fragments arranged by size:
2241 1024 979 482 248 179 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 979 1011 1315 1347 1539 1571 1644 1676 1741
Size: 32 304 32 192 32 73 32 65
Cuts at: 1741 1773 1971 2003 3055 3085 3087 3117 979
Size: 32 198 32 1052 30 2 30 3077
Fragments arranged by size:
3077 1052 304 198 192 73 65 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 834 1863 2252 834
Size: 1029 389 3797
Fragments arranged by size:
3797 1029 389
HindIII A'AGCT_T
Cuts at: 1567 1567
Size: 5215
HpaI GTT'AAC
Cuts at: 834 834
Size: 5215
KasI G'GCGC_C
Cuts at: 223 2643 2891 223
Size: 2420 248 2547
Fragments arranged by size:
2547 2420 248
KpnI G_GTAC'C
Cuts at: 2233 2233
Size: 5215
MluI A'CGCG_T
Cuts at: 4648 4648
Size: 5215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1700 3886 4070 1274
Size: 426 2186 184 2419
Fragments arranged by size:
2419 2186 426 184
MscI TGG'CCA
Cuts at: 793 964 2974 793
Size: 171 2010 3034
Fragments arranged by size:
3034 2010 171
MslI CAynn'nnrTG
Cuts at: 376 3202 3329 376
Size: 2826 127 2262
Fragments arranged by size:
2826 2262 127
MspA1I CmG'CkG
Cuts at: 855 2324 2638 2998 4013 4258 855
Size: 1469 314 360 1015 245 1812
Fragments arranged by size:
1812 1469 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3394 3394
Size: 5215
NarI GG'CG_CC
Cuts at: 224 2644 2892 224
Size: 2420 248 2547
Fragments arranged by size:
2547 2420 248
NgoMIV G'CCGG_C
Cuts at: 3392 3392
Size: 5215
NruI TCG'CGA
Cuts at: 3576 3576
Size: 5215
NspI r_CATG'y
Cuts at: 1120 1542 2266 2766 3675 1120
Size: 422 724 500 909 2660
Fragments arranged by size:
2660 909 724 500 422
PasI CC'CwG_GG
Cuts at: 915 915
Size: 5215
PciI A'CATG_T
Cuts at: 3671 3671
Size: 5215
PflMI CCAn_nnn'nTGG
Cuts at: 1249 1647 2013 1249
Size: 398 366 4451
Fragments arranged by size:
4451 398 366
PfoI T'CCnGG_A
Cuts at: 335 335
Size: 5215
PmeI GTTT'AAAC
Cuts at: 2212 2212
Size: 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 568 600 4354 4386 568
Size: 32 3754 32 1397
Fragments arranged by size:
3754 1397 32 32
PpuMI rG'GwC_Cy
Cuts at: 617 617
Size: 5215
PspOMI G'GGCC_C
Cuts at: 1852 1852
Size: 5215
PstI C_TGCA'G
Cuts at: 1434 1594 1659 2260 2945 1434
Size: 160 65 601 685 3704
Fragments arranged by size:
3704 685 601 160 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5215
PvuII CAG'CTG
Cuts at: 2638 2998 2638
Size: 360 4855
RsrII CG'GwC_CG
Cuts at: 3408 3408
Size: 5215
SacI G_AGCT'C
Cuts at: 2227 2227
Size: 5215
SalI G'TCGA_C
Cuts at: 2250 2250
Size: 5215
SanDI GG'GwC_CC
Cuts at: 617 617
Size: 5215
SapI GCTCTTCn'nnn_
Cuts at: 899 3236 3446 899
Size: 2337 210 2668
Fragments arranged by size:
2668 2337 210
SbfI CC_TGCA'GG
Cuts at: 2260 2260
Size: 5215
ScaI AGT'ACT
Cuts at: 4732 4732
Size: 5215
SexAI A'CCwGG_T
Cuts at: 1531 1531
Size: 5215
SfcI C'TryA_G
Cuts at: 33 1430 1590 1628 1655 2256 2941 3936 4127
Size: 1397 160 38 27 601 685 995 191
Cuts at: 4127 33
Size: 1121
Fragments arranged by size:
1397 1121 995 685 601 191 160 38
27
SfoI GGC'GCC
Cuts at: 225 2645 2893 225
Size: 2420 248 2547
Fragments arranged by size:
2547 2420 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5215
SmaI CCC'GGG
Cuts at: 2235 2235
Size: 5215
SmlI C'TyrA_G
Cuts at: 2368 3283 3777 4039 2368
Size: 915 494 262 3544
Fragments arranged by size:
3544 915 494 262
SnaBI TAC'GTA
Cuts at: 2205 2205
Size: 5215
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5215
SphI G_CATG'C
Cuts at: 1120 2266 2766 1120
Size: 1146 500 3569
Fragments arranged by size:
3569 1146 500
StyI C'CwwG_G
Cuts at: 1049 1511 2347 2701 1049
Size: 462 836 354 3563
Fragments arranged by size:
3563 836 462 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2796 2929 3388 3599 2796
Size: 133 459 211 4412
Fragments arranged by size:
4412 459 211 133
TatI w'GTAC_w
Cuts at: 1768 4730 1768
Size: 2962 2253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 4732 4764 509
Size: 32 4191 32 960
Fragments arranged by size:
4191 960 32 32
Tth111I GACn'n_nGTC
Cuts at: 3010 3010
Size: 5215
XbaI T'CTAG_A
Cuts at: 2244 2244
Size: 5215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 801 2708 801
Size: 1907 3308
XmaI C'CCGG_G
Cuts at: 2233 2233
Size: 5215
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
MfeI NcoI NdeI NheI NotI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI