Restriction map for
pF1KSDA0958
(Circular) (Six-base) MAPSORT of: pF1KSDA0958.seq Check: 6356 from: 1 to: 4411
pF1KSDA0958 4411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 869 869
Size: 4411
AatII G_ACGT'C
Cuts at: 275 275
Size: 4411
AccI GT'mk_AC
Cuts at: 1397 1447 1397
Size: 50 4361
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4411
AclI AA'CG_TT
Cuts at: 4251 4251
Size: 4411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 367 2234 2666 3415 3683 355
Size: 12 1867 432 749 268 1083
Fragments arranged by size:
1867 1083 749 432 268 12
AflIII A'CryG_T
Cuts at: 1761 2867 3844 1761
Size: 1106 977 2328
Fragments arranged by size:
2328 1106 977
AgeI A'CCGG_T
Cuts at: 2799 2799
Size: 4411
AhdI GACnn_n'nnGTC
Cuts at: 683 683
Size: 4411
AlwNI CAG_nnn'CTG
Cuts at: 511 621 1714 3283 511
Size: 110 1093 1569 1639
Fragments arranged by size:
1639 1569 1093 110
ApaI G_GGCC'C
Cuts at: 521 985 521
Size: 464 3947
ApaLI G'TGCA_C
Cuts at: 3181 3181
Size: 4411
ApoI r'AATT_y
Cuts at: 1413 3764 4020 1413
Size: 2351 256 1804
Fragments arranged by size:
2351 1804 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4411
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4411
AvaI C'yCGr_G
Cuts at: 1165 1429 1165
Size: 264 4147
BamHI G'GATC_C
Cuts at: 923 1434 923
Size: 511 3900
BanI G'GyrC_C
Cuts at: 732 857 1262 1425 1839 2087 2122 732
Size: 125 405 163 414 248 35 3021
Fragments arranged by size:
3021 414 405 248 163 125 35
BanII G_rGCy'C
Cuts at: 521 718 970 985 1208 1423 2453 521
Size: 197 252 15 223 215 1030 2479
Fragments arranged by size:
2479 1030 252 223 215 197 15
BbeI G_GCGC'C
Cuts at: 736 1843 2091 736
Size: 1107 248 3056
Fragments arranged by size:
3056 1107 248
BbsI GAAGACnn'nnnn_
Cuts at: 138 343 355 1093 138
Size: 205 12 738 3456
Fragments arranged by size:
3456 738 205 12
BciVI GTATCCnnnnn_n'
Cuts at: 822 2307 3070 4411 822
Size: 1485 763 1341 822
Fragments arranged by size:
1485 1341 822 763
BclI T'GATC_A
Cuts at: 612 1929 612
Size: 1317 3094
BglII A'GATC_T
Cuts at: 1924 3963 1924
Size: 2039 2372
BlpI GC'TnA_GC
Cuts at: 717 1521 717
Size: 804 3607
Bme1580I G_kGCm'C
Cuts at: 521 985 2034 2127 3185 521
Size: 464 1049 93 1058 1747
Fragments arranged by size:
1747 1058 1049 464 93
BmgBI CAC'GTC
Cuts at: 1274 1274
Size: 4411
BmrI ACTGGGnnnn_n'
Cuts at: 834 1040 1621 1702 2037 4183 834
Size: 206 581 81 335 2146 1062
Fragments arranged by size:
2146 1062 581 335 206 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 1222
Size: 4411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2958 3256 1585
Size: 879 494 298 2740
Fragments arranged by size:
2740 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 670 4115 670
Size: 3445 966
BsaAI yAC'GTr
Cuts at: 1190 1401 1762 1190
Size: 211 361 3839
Fragments arranged by size:
3839 361 211
BsaBI GATnn'nnATC
Cuts at: 6 1923 3962 6
Size: 1917 2039 455
Fragments arranged by size:
2039 1917 455
BsaHI Gr'CG_yC
Cuts at: 272 733 1840 2088 2792 4288 272
Size: 461 1107 248 704 1496 395
Fragments arranged by size:
1496 1107 704 461 395 248
BsaWI w'CCGG_w
Cuts at: 425 1598 2119 2799 3073 3220 3631 425
Size: 1173 521 680 274 147 411 1205
Fragments arranged by size:
1205 1173 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 688 1210 1381 370
Size: 318 522 171 3400
Fragments arranged by size:
3400 522 318 171
BseYI C'CCAG_C
Cuts at: 156 963 1834 2592 3171 3667 156
Size: 807 871 758 579 496 900
Fragments arranged by size:
900 871 807 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 224 604 1286 1951 1997 3207 104
Size: 120 380 682 665 46 1210 1308
Fragments arranged by size:
1308 1210 682 665 380 120 46
BsiHKAI G_wGCw'C
Cuts at: 1208 1423 2201 2391 3185 1208
Size: 215 778 190 794 2434
Fragments arranged by size:
2434 794 778 215 190
BsmBI CGTCTCn'nnnn_
Cuts at: 699 869 699
Size: 170 4241
Bsp1286I G_dGCh'C
Cuts at: 521 718 970 985 1208 1423 2034 2127 2201
Size: 197 252 15 223 215 611 93 74
Cuts at: 2201 2391 2453 3185 521
Size: 190 62 732 1747
Fragments arranged by size:
1747 732 611 252 223 215 197 190
93 74 62 15
BspEI T'CCGG_A
Cuts at: 425 3631 425
Size: 3206 1205
BspHI T'CATG_A
Cuts at: 1082 3587 1082
Size: 2505 1906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 869 1459 1673 1975 2356 305
Size: 564 590 214 302 381 2360
Fragments arranged by size:
2360 590 564 381 302 214
BsrBI CCG'CTC
Cuts at: 50 600 1292 2701 2755 4241 4406 50
Size: 550 692 1409 54 1486 165 55
Fragments arranged by size:
1486 1409 692 550 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4411
BsrFI r'CCGG_y
Cuts at: 1094 2407 2588 2799 1094
Size: 1313 181 211 2706
Fragments arranged by size:
2706 1313 211 181
BssSI C'ACGA_G
Cuts at: 640 2680 3040 640
Size: 2040 360 2011
Fragments arranged by size:
2040 2011 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4411
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4411
BstYI r'GATC_y
Cuts at: 1 923 1070 1244 1434 1901 1924 2259 2505
Size: 922 147 174 190 467 23 335 246
Cuts at: 2505 3519 3605 3617 3963 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 922 467 449 346 335 246 190
174 147 86 23 12
BtgI C'CryG_G
Cuts at: 125 379 905 125
Size: 254 526 3631
Fragments arranged by size:
3631 526 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 2359 2540 2703 486
Size: 1873 181 163 2194
Fragments arranged by size:
2194 1873 181 163
BtsI GCAGTG_nn'
Cuts at: 170 1652 170
Size: 1482 2929
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4411
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2975 2115
Size: 860 3551
EaeI y'GGCC_r
Cuts at: 192 221 601 1115 1283 1994 2168 2559 2586
Size: 29 380 514 168 711 174 391 27
Cuts at: 2586 3933 192
Size: 1347 670
Fragments arranged by size:
1347 711 670 514 391 380 174 168
29 27
EagI C'GGCC_G
Cuts at: 221 601 1283 1994 221
Size: 380 682 711 2638
Fragments arranged by size:
2638 711 682 380
EarI CTCTTCn'nnn_
Cuts at: 578 1139 2432 2642 578
Size: 561 1293 210 2347
Fragments arranged by size:
2347 1293 561 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2929 3075 4221 2617
Size: 312 146 1146 2807
Fragments arranged by size:
2807 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1206 1421 1206
Size: 215 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 367 1222 2234 2666 3415 3683 355
Size: 12 855 1012 432 749 268 1083
Fragments arranged by size:
1083 1012 855 749 432 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 865 865
Size: 4411
EcoO109I rG'GnC_Cy
Cuts at: 518 575 981 982 1548 518
Size: 57 406 1 566 3381
Fragments arranged by size:
3381 566 406 57 1
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4411
FspI TGC'GCA
Cuts at: 301 1251 2190 301
Size: 950 939 2522
Fragments arranged by size:
2522 950 939
HaeII r_GCGC'y
Cuts at: 736 1843 2091 3115 4094 736
Size: 1107 248 1024 979 1053
Fragments arranged by size:
1107 1053 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 669 701 879 911 1279 1311 2251
Size: 32 420 32 178 32 368 32 940
Cuts at: 2251 2281 2283 2313 217
Size: 30 2 30 2315
Fragments arranged by size:
2315 940 420 368 178 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 276 1448 276
Size: 1172 3239
KasI G'GCGC_C
Cuts at: 732 1839 2087 732
Size: 1107 248 3056
Fragments arranged by size:
3056 1107 248
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4411
MluI A'CGCG_T
Cuts at: 3844 3844
Size: 4411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3082 3266 3082
Size: 184 4227
MscI TGG'CCA
Cuts at: 1117 2170 1117
Size: 1053 3358
MslI CAynn'nnrTG
Cuts at: 2398 2525 2398
Size: 127 4284
MspA1I CmG'CkG
Cuts at: 1520 1834 2194 3209 3454 1520
Size: 314 360 1015 245 2477
Fragments arranged by size:
2477 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4411
NarI GG'CG_CC
Cuts at: 733 1840 2088 733
Size: 1107 248 3056
Fragments arranged by size:
3056 1107 248
NcoI C'CATG_G
Cuts at: 125 379 125
Size: 254 4157
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4411
NruI TCG'CGA
Cuts at: 2772 2772
Size: 4411
NspI r_CATG'y
Cuts at: 1462 1962 2871 1462
Size: 500 909 3002
Fragments arranged by size:
3002 909 500
PciI A'CATG_T
Cuts at: 2867 2867
Size: 4411
PflMI CCAn_nnn'nTGG
Cuts at: 1098 1098
Size: 4411
PfoI T'CCnGG_A
Cuts at: 932 932
Size: 4411
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4411
PmlI CAC'GTG
Cuts at: 1190 1190
Size: 4411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3582 3550
Size: 32 4379
PpuMI rG'GwC_Cy
Cuts at: 575 575
Size: 4411
PspOMI G'GGCC_C
Cuts at: 517 981 517
Size: 464 3947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 481 513 481
Size: 32 4379
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3726
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4411
PvuII CAG'CTG
Cuts at: 1834 2194 1834
Size: 360 4051
RsrII CG'GwC_CG
Cuts at: 764 2604 764
Size: 1840 2571
SacI G_AGCT'C
Cuts at: 1208 1423 1208
Size: 215 4196
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4411
SapI GCTCTTCn'nnn_
Cuts at: 1139 2432 2642 1139
Size: 1293 210 2908
Fragments arranged by size:
2908 1293 210
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4411
ScaI AGT'ACT
Cuts at: 646 1330 3928 646
Size: 684 2598 1129
Fragments arranged by size:
2598 1129 684
SexAI A'CCwGG_T
Cuts at: 419 530 419
Size: 111 4300
SfcI C'TryA_G
Cuts at: 33 648 1452 2137 3132 3323 33
Size: 615 804 685 995 191 1121
Fragments arranged by size:
1121 995 804 685 615 191
SfoI GGC'GCC
Cuts at: 734 1841 2089 734
Size: 1107 248 3056
Fragments arranged by size:
3056 1107 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4411
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4411
SmlI C'TyrA_G
Cuts at: 1564 2479 2973 3235 1564
Size: 915 494 262 2740
Fragments arranged by size:
2740 915 494 262
SnaBI TAC'GTA
Cuts at: 1401 1401
Size: 4411
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4411
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3911
StyI C'CwwG_G
Cuts at: 125 379 1543 1897 125
Size: 254 1164 354 2639
Fragments arranged by size:
2639 1164 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 351 1992 2125 2584 2795 351
Size: 1641 133 459 211 1967
Fragments arranged by size:
1967 1641 459 211 133
TatI w'GTAC_w
Cuts at: 623 644 1328 3926 623
Size: 21 684 2598 1108
Fragments arranged by size:
2598 1108 684 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3928 3960 3928
Size: 32 4379
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4411
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4411
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4411
ZraI GAC'GTC
Cuts at: 273 273
Size: 4411
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PshAI
PsiI PspXI SacII SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI