Restriction map for
pF1KSDA0959
(Circular) (Six-base) MAPSORT of: pF1KSDA0959.seq Check: 5989 from: 1 to: 5441
pF1KSDA0959 5441 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2080 2080
Size: 5441
AatII G_ACGT'C
Cuts at: 2020 2020
Size: 5441
AccI GT'mk_AC
Cuts at: 1083 2479 1083
Size: 1396 4045
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5441
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 1111 1411 2197 3266 3698 4445 4713 744
Size: 367 300 786 1069 432 747 268 1472
Fragments arranged by size:
1472 1069 786 747 432 367 300 268
AfeI AGC'GCT
Cuts at: 2002 2002
Size: 5441
AflIII A'CryG_T
Cuts at: 2111 2793 3897 4874 2111
Size: 682 1104 977 2678
Fragments arranged by size:
2678 1104 977 682
AgeI A'CCGG_T
Cuts at: 3829 3829
Size: 5441
AhdI GACnn_n'nnGTC
Cuts at: 295 295
Size: 5441
AlwNI CAG_nnn'CTG
Cuts at: 636 1581 1630 1822 2746 4313 636
Size: 945 49 192 924 1567 1764
Fragments arranged by size:
1764 1567 945 924 192 49
ApaLI G'TGCA_C
Cuts at: 4211 4211
Size: 5441
ApoI r'AATT_y
Cuts at: 805 1095 1279 1477 1923 2445 4794 5050 805
Size: 290 184 198 446 522 2349 256 1196
Fragments arranged by size:
2349 1196 522 446 290 256 198 184
AseI AT'TA_AT
Cuts at: 20 20
Size: 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 2461 2461
Size: 5441
BamHI G'GATC_C
Cuts at: 2466 2466
Size: 5441
BanI G'GyrC_C
Cuts at: 340 1387 2457 2871 3119 3154 340
Size: 1047 1070 414 248 35 2627
Fragments arranged by size:
2627 1070 1047 414 248 35
BanII G_rGCy'C
Cuts at: 157 611 670 1765 1834 2455 3485 157
Size: 454 59 1095 69 621 1030 2113
Fragments arranged by size:
2113 1095 1030 621 454 69 59
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5193
BbsI GAAGACnn'nnnn_
Cuts at: 602 1931 2072 2102 602
Size: 1329 141 30 3941
Fragments arranged by size:
3941 1329 141 30
BbvCI CC'TCA_GC
Cuts at: 1823 1823
Size: 5441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2019 2053 2019
Size: 34 5407
BciVI GTATCCnnnnn_n'
Cuts at: 3339 4100 5441 3339
Size: 761 1341 3339
Fragments arranged by size:
3339 1341 761
BclI T'GATC_A
Cuts at: 542 1709 2961 542
Size: 1167 1252 3022
Fragments arranged by size:
3022 1252 1167
BglII A'GATC_T
Cuts at: 825 2956 4993 825
Size: 2131 2037 1273
Fragments arranged by size:
2131 2037 1273
BlpI GC'TnA_GC
Cuts at: 2553 2553
Size: 5441
Bme1580I G_kGCm'C
Cuts at: 1379 1390 3066 3159 4215 1379
Size: 11 1676 93 1056 2605
Fragments arranged by size:
2605 1676 1056 93 11
BmrI ACTGGGnnnn_n'
Cuts at: 177 2653 2734 3069 5213 177
Size: 2476 81 335 2144 405
Fragments arranged by size:
2476 2144 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1848 1905 3808 1848
Size: 57 1903 3481
Fragments arranged by size:
3481 1903 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 805 1327 2421 269
Size: 536 522 1094 3289
Fragments arranged by size:
3289 1094 536 522
Bpu10I CC'TnA_GC
Cuts at: 1823 1823
Size: 5441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1128 2617 3496 3988 4286 721
Size: 407 1489 879 492 298 1876
Fragments arranged by size:
1876 1489 879 492 407 298
BsaI GGTCTCn'nnnn_
Cuts at: 914 5145 914
Size: 4231 1210
BsaAI yAC'GTr
Cuts at: 812 2433 2794 812
Size: 1621 361 3459
Fragments arranged by size:
3459 1621 361
BsaBI GATnn'nnATC
Cuts at: 6 1066 2955 4992 6
Size: 1060 1889 2037 455
Fragments arranged by size:
2037 1889 1060 455
BsaHI Gr'CG_yC
Cuts at: 1634 2017 2872 3120 3822 5318 1634
Size: 383 855 248 702 1496 1757
Fragments arranged by size:
1757 1496 855 702 383 248
BsaWI w'CCGG_w
Cuts at: 2630 3151 3829 4103 4250 4661 2630
Size: 521 678 274 147 411 3410
Fragments arranged by size:
3410 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 739 769 1752 1782 1942 1972 739
Size: 30 983 30 160 30 4208
Fragments arranged by size:
4208 983 160 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1603 1606 1840 1878 1950 2221 1603
Size: 3 234 38 72 271 4823
Fragments arranged by size:
4823 271 234 72 38 3
BseYI C'CCAG_C
Cuts at: 2866 3624 4201 4697 2866
Size: 758 577 496 3610
Fragments arranged by size:
3610 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 2242 611
Size: 1631 3810
BsiEI CG_ry'CG
Cuts at: 104 2983 3029 4237 104
Size: 2879 46 1208 1308
Fragments arranged by size:
2879 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 157 690 1765 2455 3233 3423 4215 157
Size: 533 1075 690 778 190 792 1383
Fragments arranged by size:
1383 1075 792 778 690 533 190
BsmI GAATG_Cn'
Cuts at: 555 1193 555
Size: 638 4803
Bsp1286I G_dGCh'C
Cuts at: 157 611 670 690 1379 1390 1765 1834 2455
Size: 454 59 20 689 11 375 69 621
Cuts at: 2455 3066 3159 3233 3423 3485 4215 157
Size: 611 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 689 621 611 454 375 190
93 74 69 62 59 20 11
BspEI T'CCGG_A
Cuts at: 4661 4661
Size: 5441
BspHI T'CATG_A
Cuts at: 1074 4617 1074
Size: 3543 1898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 2080 2214 2491 2705 3007 3388 374
Size: 1706 134 277 214 302 381 2427
Fragments arranged by size:
2427 1706 381 302 277 214 134
BsrBI CCG'CTC
Cuts at: 50 3733 3787 5271 5436 50
Size: 3683 54 1484 165 55
Fragments arranged by size:
3683 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3353 3353
Size: 5441
BsrFI r'CCGG_y
Cuts at: 3439 3620 3829 3439
Size: 181 209 5051
Fragments arranged by size:
5051 209 181
BsrGI T'GTAC_A
Cuts at: 2114 2114
Size: 5441
BssSI C'ACGA_G
Cuts at: 829 3712 4070 829
Size: 2883 358 2200
Fragments arranged by size:
2883 2200 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2818 2818
Size: 5441
BstBI TT'CG_AA
Cuts at: 110 3802 110
Size: 3692 1749
BstYI r'GATC_y
Cuts at: 1 825 2466 2933 2956 3291 3537 4549 4635
Size: 824 1641 467 23 335 246 1012 86
Cuts at: 4635 4647 4993 1
Size: 12 346 449
Fragments arranged by size:
1641 1012 824 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1929 1929
Size: 5441
BtgI C'CryG_G
Cuts at: 1844 1913 2330 1844
Size: 69 417 4955
Fragments arranged by size:
4955 417 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1053 3391 3572 3735 1053
Size: 2338 181 163 2759
Fragments arranged by size:
2759 2338 181 163
BtsI GCAGTG_nn'
Cuts at: 888 1126 1305 1418 1748 2684 888
Size: 238 179 113 330 936 3645
Fragments arranged by size:
3645 936 330 238 179 113
DraI TTT'AAA
Cuts at: 2440 2440
Size: 5441
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4005 3147
Size: 858 4583
EaeI y'GGCC_r
Cuts at: 3026 3200 3591 3618 4963 3026
Size: 174 391 27 1345 3504
Fragments arranged by size:
3504 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3026 3026
Size: 5441
EarI CTCTTCn'nnn_
Cuts at: 170 678 764 770 871 1657 1669 3464 3674
Size: 508 86 6 101 786 12 1795 210
Cuts at: 3674 170
Size: 1937
Fragments arranged by size:
1937 1795 786 508 210 101 86 12
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3959 4105 5251 3649
Size: 310 146 1146 3839
Fragments arranged by size:
3839 1146 310 146
EcoICRI GAG'CTC
Cuts at: 155 1763 2453 155
Size: 1608 690 3143
Fragments arranged by size:
3143 1608 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 744 805 1111 1327 1411 2197 2421 3266
Size: 475 61 306 216 84 786 224 845
Cuts at: 3266 3698 4445 4713 269
Size: 432 747 268 997
Fragments arranged by size:
997 845 786 747 475 432 306 268
224 216 84 61
EcoNI CCTnn'n_nnAGG
Cuts at: 769 1107 1585 769
Size: 338 478 4625
Fragments arranged by size:
4625 478 338
EcoO109I rG'GnC_Cy
Cuts at: 572 2580 572
Size: 2008 3433
EcoRI G'AATT_C
Cuts at: 805 2445 805
Size: 1640 3801
EcoRV GAT'ATC
Cuts at: 1215 1215
Size: 5441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5441
FspI TGC'GCA
Cuts at: 2317 3222 2317
Size: 905 4536
HaeII r_GCGC'y
Cuts at: 2004 2875 3123 4145 5124 2004
Size: 871 248 1022 979 2321
Fragments arranged by size:
2321 1022 979 871 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 1031 1063 1204 1236 1701 1733 3283
Size: 32 718 32 141 32 465 32 1550
Cuts at: 3283 3313 3315 3345 281
Size: 30 2 30 2377
Fragments arranged by size:
2377 1550 718 465 141 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2132 2480 2132
Size: 348 5093
HindIII A'AGCT_T
Cuts at: 352 352
Size: 5441
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5193
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5441
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4112 4296 4112
Size: 184 5257
MscI TGG'CCA
Cuts at: 3202 3202
Size: 5441
MslI CAynn'nnrTG
Cuts at: 655 1357 2089 3430 3557 655
Size: 702 732 1341 127 2539
Fragments arranged by size:
2539 1341 732 702 127
MspA1I CmG'CkG
Cuts at: 279 843 1627 1757 2155 2552 2866 3226 4239
Size: 564 784 130 398 397 314 360 1013
Cuts at: 4239 4484 279
Size: 245 1236
Fragments arranged by size:
1236 1013 784 564 398 397 360 314
245 130
NaeI GCC'GGC
Cuts at: 3622 3622
Size: 5441
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5193
NcoI C'CATG_G
Cuts at: 1844 1913 2330 1844
Size: 69 417 4955
Fragments arranged by size:
4955 417 69
NgoMIV G'CCGG_C
Cuts at: 3620 3620
Size: 5441
NspI r_CATG'y
Cuts at: 2115 2494 2994 3901 2115
Size: 379 500 907 3655
Fragments arranged by size:
3655 907 500 379
PciI A'CATG_T
Cuts at: 2111 3897 2111
Size: 1786 3655
PflMI CCAn_nnn'nTGG
Cuts at: 894 1914 894
Size: 1020 4421
PfoI T'CCnGG_A
Cuts at: 285 285
Size: 5441
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PmlI CAC'GTG
Cuts at: 812 812
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 4580
Size: 32 5409
PstI C_TGCA'G
Cuts at: 1133 1340 2488 3173 1133
Size: 207 1148 685 3401
Fragments arranged by size:
3401 1148 685 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 279 843 1627 2866 3226 279
Size: 564 784 1239 360 2494
Fragments arranged by size:
2494 1239 784 564 360
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5441
SacI G_AGCT'C
Cuts at: 157 1765 2455 157
Size: 1608 690 3143
Fragments arranged by size:
3143 1608 690
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5441
SapI GCTCTTCn'nnn_
Cuts at: 678 1657 3464 3674 678
Size: 979 1807 210 2445
Fragments arranged by size:
2445 1807 979 210
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5441
ScaI AGT'ACT
Cuts at: 4958 4958
Size: 5441
SfcI C'TryA_G
Cuts at: 33 1129 1336 2484 3169 4162 4353 33
Size: 1096 207 1148 685 993 191 1121
Fragments arranged by size:
1148 1121 1096 993 685 207 191
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5193
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SmaI CCC'GGG
Cuts at: 2463 2463
Size: 5441
SmlI C'TyrA_G
Cuts at: 700 1107 2596 3511 4003 4265 700
Size: 407 1489 915 492 262 1876
Fragments arranged by size:
1876 1489 915 492 407 262
SnaBI TAC'GTA
Cuts at: 2433 2433
Size: 5441
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5441
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4941
StyI C'CwwG_G
Cuts at: 344 1177 1844 1913 2330 2575 2929 344
Size: 833 667 69 417 245 354 2856
Fragments arranged by size:
2856 833 667 417 354 245 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1199 1345 3024 3157 3616 3825 1199
Size: 146 1679 133 459 209 2815
Fragments arranged by size:
2815 1679 459 209 146 133
TatI w'GTAC_w
Cuts at: 2114 4956 2114
Size: 2842 2599
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1752 1784 4958 4990 1752
Size: 32 3174 32 2203
Fragments arranged by size:
3174 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1783 3238 1783
Size: 1455 3986
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1842 2936 1842
Size: 1094 4347
XmaI C'CCGG_G
Cuts at: 2461 2461
Size: 5441
ZraI GAC'GTC
Cuts at: 2018 2018
Size: 5441
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AleI AlfI AloI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI