Restriction map for
pF1KSDA0961
(Circular) (Six-base) MAPSORT of: pF1KSDA0961.seq Check: 5532 from: 1 to: 4694
pF1KSDA0961 4694 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 164 732 1732 164
Size: 568 1000 3126
Fragments arranged by size:
3126 1000 568
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2951 3698 3966 2519
Size: 432 747 268 3247
Fragments arranged by size:
3247 747 432 268
AflIII A'CryG_T
Cuts at: 796 2046 3150 4127 796
Size: 1250 1104 977 1363
Fragments arranged by size:
1363 1250 1104 977
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AhdI GACnn_n'nnGTC
Cuts at: 1214 1214
Size: 4694
AleI CACnn'nnGTG
Cuts at: 920 1172 920
Size: 252 4442
AlwNI CAG_nnn'CTG
Cuts at: 996 1999 3566 996
Size: 1003 1567 2124
Fragments arranged by size:
2124 1567 1003
ApaLI G'TGCA_C
Cuts at: 3464 3464
Size: 4694
ApoI r'AATT_y
Cuts at: 210 659 743 827 1163 1583 1667 1698 4047
Size: 449 84 84 336 420 84 31 2349
Cuts at: 4047 4303 210
Size: 256 601
Fragments arranged by size:
2349 601 449 420 336 256 84 84
84 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1714 1714
Size: 4694
BamHI G'GATC_C
Cuts at: 1719 1719
Size: 4694
BanI G'GyrC_C
Cuts at: 1710 2124 2372 2407 1710
Size: 414 248 35 3997
Fragments arranged by size:
3997 414 248 35
BanII G_rGCy'C
Cuts at: 320 1708 2738 320
Size: 1388 1030 2276
Fragments arranged by size:
2276 1388 1030
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 1213 1465 1213
Size: 252 4442
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 290 2214 290
Size: 1924 2770
BglII A'GATC_T
Cuts at: 788 2209 4246 788
Size: 1421 2037 1236
Fragments arranged by size:
2037 1421 1236
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 723 2319 2412 3468 723
Size: 1596 93 1056 1949
Fragments arranged by size:
1949 1596 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1906 1987 2322 4466 1906
Size: 81 335 2144 2134
Fragments arranged by size:
2144 2134 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 4694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1109
Size: 4694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2749 3241 3539 1870
Size: 879 492 298 3025
Fragments arranged by size:
3025 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4398 4398
Size: 4694
BsaAI yAC'GTr
Cuts at: 1686 2047 1686
Size: 361 4333
BsaBI GATnn'nnATC
Cuts at: 6 2208 4245 6
Size: 2202 2037 455
Fragments arranged by size:
2202 2037 455
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3356 3503 3914 1883
Size: 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 884 914 1136 1166 884
Size: 30 222 30 4412
Fragments arranged by size:
4412 222 30 30
BseYI C'CCAG_C
Cuts at: 2119 2877 3454 3950 2119
Size: 758 577 496 2863
Fragments arranged by size:
2863 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1708 2486 2676 3468 1708
Size: 778 190 792 2934
Fragments arranged by size:
2934 792 778 190
BsmI GAATG_Cn'
Cuts at: 193 193
Size: 4694
Bsp1286I G_dGCh'C
Cuts at: 320 723 1708 2319 2412 2486 2676 2738 3468
Size: 403 985 611 93 74 190 62 730
Cuts at: 3468 320
Size: 1546
Fragments arranged by size:
1546 985 730 611 403 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3914 3914
Size: 4694
BspHI T'CATG_A
Cuts at: 486 3870 486
Size: 3384 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1001 2606 1001
Size: 1605 3089
BsrFI r'CCGG_y
Cuts at: 2692 2873 3082 2692
Size: 181 209 4304
Fragments arranged by size:
4304 209 181
BsrGI T'GTAC_A
Cuts at: 215 215
Size: 4694
BssSI C'ACGA_G
Cuts at: 131 2965 3323 131
Size: 2834 358 1502
Fragments arranged by size:
2834 1502 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2071
Size: 4694
BstBI TT'CG_AA
Cuts at: 110 3055 110
Size: 2945 1749
BstYI r'GATC_y
Cuts at: 1 788 1719 2186 2209 2544 2790 3802 3888
Size: 787 931 467 23 335 246 1012 86
Cuts at: 3888 3900 4246 1
Size: 12 346 449
Fragments arranged by size:
1012 931 787 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2644 2825 2988 2644
Size: 181 163 4350
Fragments arranged by size:
4350 181 163
BtsI GCAGTG_nn'
Cuts at: 1937 1937
Size: 4694
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 2279 2453 2844 2871 4216 2279
Size: 174 391 27 1345 2757
Fragments arranged by size:
2757 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 563 2717 2927 563
Size: 2154 210 2330
Fragments arranged by size:
2330 2154 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1706 1706
Size: 4694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 2519 2951 3698 3966 1109
Size: 1410 432 747 268 1837
Fragments arranged by size:
1837 1410 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1833 1833
Size: 4694
EcoRI G'AATT_C
Cuts at: 659 743 827 1163 1583 1667 1698 659
Size: 84 84 336 420 84 31 3655
Fragments arranged by size:
3655 420 336 84 84 84 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4694
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3398 4377 2128
Size: 248 1022 979 2445
Fragments arranged by size:
2445 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 1200 1232 1560 1592 2536 2566 2568
Size: 32 816 32 328 32 944 30 2
Cuts at: 2568 2598 352
Size: 30 2448
Fragments arranged by size:
2448 944 816 328 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 733 1733 733
Size: 1000 3694
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3365 3549 3365
Size: 184 4510
MscI TGG'CCA
Cuts at: 2455 2455
Size: 4694
MslI CAynn'nnrTG
Cuts at: 668 836 920 1172 1256 1424 1508 1592 1608
Size: 168 84 252 84 168 84 84 16
Cuts at: 1608 2683 2810 668
Size: 1075 127 2552
Fragments arranged by size:
2552 1075 252 168 168 127 84 84
84 84 16
MspA1I CmG'CkG
Cuts at: 1805 2119 2479 3492 3737 1805
Size: 314 360 1013 245 2762
Fragments arranged by size:
2762 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NcoI C'CATG_G
Cuts at: 125 125
Size: 4694
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NspI r_CATG'y
Cuts at: 800 1747 2247 3154 800
Size: 947 500 907 2340
Fragments arranged by size:
2340 947 907 500
PciI A'CATG_T
Cuts at: 796 3150 796
Size: 2354 2340
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 3833 3865 114
Size: 32 3687 32 943
Fragments arranged by size:
3687 943 32 32
PstI C_TGCA'G
Cuts at: 1741 2426 1741
Size: 685 4009
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 2119 2479 2119
Size: 360 4334
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1708 1708
Size: 4694
SalI G'TCGA_C
Cuts at: 731 1731 731
Size: 1000 3694
SapI GCTCTTCn'nnn_
Cuts at: 2717 2927 2717
Size: 210 4484
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 161 240 1737 2422 3415 3606 33
Size: 128 79 1497 685 993 191 1121
Fragments arranged by size:
1497 1121 993 685 191 128 79
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 1849 2764 3256 3518 1849
Size: 915 492 262 3025
Fragments arranged by size:
3025 915 492 262
SnaBI TAC'GTA
Cuts at: 1686 1686
Size: 4694
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1747 2247 1747
Size: 500 4194
StuI AGG'CCT
Cuts at: 958 1042 1061 1126 1546 958
Size: 84 19 65 420 4106
Fragments arranged by size:
4106 420 84 65 19
StyI C'CwwG_G
Cuts at: 125 1828 2182 125
Size: 1703 354 2637
Fragments arranged by size:
2637 1703 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2277 2410 2869 3078 2277
Size: 133 459 209 3893
Fragments arranged by size:
3893 459 209 133
TatI w'GTAC_w
Cuts at: 215 816 1054 4209 215
Size: 601 238 3155 700
Fragments arranged by size:
3155 700 601 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 543 575 4211 4243 543
Size: 32 3636 32 994
Fragments arranged by size:
3636 994 32 32
Tth111I GACn'n_nGTC
Cuts at: 2491 2491
Size: 4694
XbaI T'CTAG_A
Cuts at: 357 1725 357
Size: 1368 3326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
XmnI GAAnn'nnTTC
Cuts at: 1210 1587 1210
Size: 377 4317
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI