Restriction map for
pF1KSDA0965
(Circular) (Six-base) MAPSORT of: pF1KSDA0965.seq Check: 9049 from: 1 to: 4529
pF1KSDA0965 4529 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 507
Size: 4529
AccI GT'mk_AC
Cuts at: 1283 1567 1283
Size: 284 4245
Acc65I G'GTAC_C
Cuts at: 1167 1545 1167
Size: 378 4151
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2786 3533 3801 2354
Size: 432 747 268 3082
Fragments arranged by size:
3082 747 432 268
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AhdI GACnn_n'nnGTC
Cuts at: 396 1044 396
Size: 648 3881
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 682
Size: 32 4497
AlwNI CAG_nnn'CTG
Cuts at: 715 1834 3401 715
Size: 1119 1567 1843
Fragments arranged by size:
1843 1567 1119
ApaLI G'TGCA_C
Cuts at: 3299 3299
Size: 4529
ApoI r'AATT_y
Cuts at: 213 628 859 1353 1533 3882 4138 213
Size: 415 231 494 180 2349 256 604
Fragments arranged by size:
2349 604 494 415 256 231 180
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 176 1549 176
Size: 1373 3156
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 565 1167 1545 1959 2207 2242 565
Size: 602 378 414 248 35 2852
Fragments arranged by size:
2852 602 414 378 248 35
BanII G_rGCy'C
Cuts at: 1543 2573 1543
Size: 1030 3499
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglII A'GATC_T
Cuts at: 2044 4081 2044
Size: 2037 2492
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 2154 2247 3303 2154
Size: 93 1056 3380
Fragments arranged by size:
3380 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1296 1440 1741 1822 2157 4301 1296
Size: 144 301 81 335 2144 1524
Fragments arranged by size:
2144 1524 335 301 144 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 983 1145 659
Size: 324 162 4043
Fragments arranged by size:
4043 324 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1705 2584 3076 3374 1094
Size: 611 879 492 298 2249
Fragments arranged by size:
2249 879 611 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4233 4233
Size: 4529
BsaAI yAC'GTr
Cuts at: 1521 1882 1521
Size: 361 4168
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1960 2208 2910 4406 1960
Size: 248 702 1496 2083
Fragments arranged by size:
2083 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1410 1718 2239 2917 3191 3338 3749 1410
Size: 308 521 678 274 147 411 2190
Fragments arranged by size:
2190 678 521 411 308 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 434 434
Size: 4529
BseYI C'CCAG_C
Cuts at: 1507 1954 2712 3289 3785 1507
Size: 447 758 577 496 2251
Fragments arranged by size:
2251 758 577 496 447
BsiEI CG_ry'CG
Cuts at: 104 2071 2117 3325 104
Size: 1967 46 1208 1308
Fragments arranged by size:
1967 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1295 1543 2321 2511 3303 1295
Size: 248 778 190 792 2521
Fragments arranged by size:
2521 792 778 248 190
Bsp1286I G_dGCh'C
Cuts at: 1295 1543 2154 2247 2321 2511 2573 3303 1295
Size: 248 611 93 74 190 62 730 2521
Fragments arranged by size:
2521 730 611 248 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 507 1579 1793 2095 2476 507
Size: 1072 214 302 381 2560
Fragments arranged by size:
2560 1072 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2821 2875 4359 4524 50
Size: 2771 54 1484 165 55
Fragments arranged by size:
2771 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 137 482 655 992 2441 137
Size: 345 173 337 1449 2225
Fragments arranged by size:
2225 1449 345 337 173
BsrFI r'CCGG_y
Cuts at: 2527 2708 2917 2527
Size: 181 209 4139
Fragments arranged by size:
4139 209 181
BssSI C'ACGA_G
Cuts at: 2800 3158 2800
Size: 358 4171
BstAPI GCAn_nnn'nTGC
Cuts at: 565 730 1906 565
Size: 165 1176 3188
Fragments arranged by size:
3188 1176 165
BstBI TT'CG_AA
Cuts at: 110 2890 110
Size: 2780 1749
BstYI r'GATC_y
Cuts at: 1 1554 2021 2044 2379 2625 3637 3723 3735
Size: 1553 467 23 335 246 1012 86 12
Cuts at: 3735 4081 1
Size: 346 449
Fragments arranged by size:
1553 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 281 125
Size: 156 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 1772 1772
Size: 4529
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DraIII CAC_nnn'GTG
Cuts at: 892 892
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 278 2114 2288 2679 2706 4051 278
Size: 1836 174 391 27 1345 756
Fragments arranged by size:
1836 1345 756 391 174 27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 239 302 411 680 2552 2762 239
Size: 63 109 269 1872 210 2006
Fragments arranged by size:
2006 1872 269 210 109 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 3047 3193 4339 2737
Size: 310 146 1146 2927
Fragments arranged by size:
2927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 983 1145 2354 2786 3533 3801 659
Size: 324 162 1209 432 747 268 1387
Fragments arranged by size:
1387 1209 747 432 324 268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 1455 1455
Size: 4529
EcoO109I rG'GnC_Cy
Cuts at: 1668 1668
Size: 4529
EcoRI G'AATT_C
Cuts at: 1533 1533
Size: 4529
EcoRV GAT'ATC
Cuts at: 1084 1084
Size: 4529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 440 2548 440
Size: 2108 2421
FspI TGC'GCA
Cuts at: 2310 2310
Size: 4529
HaeII r_GCGC'y
Cuts at: 1963 2211 3233 4212 1963
Size: 248 1022 979 2280
Fragments arranged by size:
2280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 1030 1062 1405 1437 2371 2401 2403
Size: 32 616 32 343 32 934 30 2
Cuts at: 2403 2433 382
Size: 30 2478
Fragments arranged by size:
2478 934 616 343 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1284 1472 1568 1284
Size: 188 96 4245
Fragments arranged by size:
4245 188 96
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1171 1549 1171
Size: 378 4151
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3200 3384 3200
Size: 184 4345
MscI TGG'CCA
Cuts at: 280 2290 280
Size: 2010 2519
MslI CAynn'nnrTG
Cuts at: 130 470 1498 2518 2645 130
Size: 340 1028 1020 127 2014
Fragments arranged by size:
2014 1028 1020 340 127
MspA1I CmG'CkG
Cuts at: 1640 1954 2314 3327 3572 1640
Size: 314 360 1013 245 2597
Fragments arranged by size:
2597 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NcoI C'CATG_G
Cuts at: 125 281 125
Size: 156 4373
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 1582 2082 2989 1582
Size: 500 907 3122
Fragments arranged by size:
3122 907 500
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PflMI CCAn_nnn'nTGG
Cuts at: 744 1430 744
Size: 686 3843
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 901 933 3668 3700 901
Size: 32 2735 32 1730
Fragments arranged by size:
2735 1730 32 32
PstI C_TGCA'G
Cuts at: 1576 2261 1576
Size: 685 3844
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 1954 2314 1954
Size: 360 4169
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1282 1566 1282
Size: 284 4245
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 191 1322 1572 2257 3250 3441 33
Size: 158 1131 250 685 993 191 1121
Fragments arranged by size:
1131 1121 993 685 250 191 158
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 178 1551 178
Size: 1373 3156
SmlI C'TyrA_G
Cuts at: 1109 1684 2599 3091 3353 1109
Size: 575 915 492 262 2285
Fragments arranged by size:
2285 915 575 492 262
SnaBI TAC'GTA
Cuts at: 1521 1521
Size: 4529
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1582 2082 1582
Size: 500 4029
StyI C'CwwG_G
Cuts at: 125 281 794 1187 1663 2017 125
Size: 156 513 393 476 354 2637
Fragments arranged by size:
2637 513 476 393 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 561 2112 2245 2704 2913 561
Size: 1551 133 459 209 2177
Fragments arranged by size:
2177 1551 459 209 133
TatI w'GTAC_w
Cuts at: 4044 4044
Size: 4529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4046 4078 4046
Size: 32 4497
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 204 381 2024 204
Size: 177 1643 2709
Fragments arranged by size:
2709 1643 177
XmaI C'CCGG_G
Cuts at: 176 1549 176
Size: 1373 3156
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI