Restriction map for
pF1KSDA0967
(Circular) (Six-base) MAPSORT of: pF1KSDA0967.seq Check: 6290 from: 1 to: 7873
pF1KSDA0967 7873 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 842 852 1858 1990 842
Size: 10 1006 132 6725
Fragments arranged by size:
6725 1006 132 10
AatII G_ACGT'C
Cuts at: 1523 1523
Size: 7873
AccI GT'mk_AC
Cuts at: 2714 4909 2714
Size: 2195 5678
Acc65I G'GTAC_C
Cuts at: 4887 4887
Size: 7873
AclI AA'CG_TT
Cuts at: 7713 7713
Size: 7873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 601 632 888 1498 4058 5696 6128 6877
Size: 131 31 256 610 2560 1638 432 749
Cuts at: 6877 7145 470
Size: 268 1198
Fragments arranged by size:
2560 1638 1198 749 610 432 268 256
131 31
AfeI AGC'GCT
Cuts at: 1009 1009
Size: 7873
AflIII A'CryG_T
Cuts at: 2720 5223 6329 7306 2720
Size: 2503 1106 977 3287
Fragments arranged by size:
3287 2503 1106 977
AgeI A'CCGG_T
Cuts at: 6261 6261
Size: 7873
AleI CACnn'nnGTG
Cuts at: 730 730
Size: 7873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1012 1012
Size: 7873
AlwNI CAG_nnn'CTG
Cuts at: 911 1691 1850 3067 3200 4614 5176 6745 911
Size: 780 159 1217 133 1414 562 1569 2039
Fragments arranged by size:
2039 1569 1414 1217 780 562 159 133
ApaI G_GGCC'C
Cuts at: 254 1843 2486 254
Size: 1589 643 5641
Fragments arranged by size:
5641 1589 643
ApaLI G'TGCA_C
Cuts at: 601 2759 4476 4797 6643 601
Size: 2158 1717 321 1846 1831
Fragments arranged by size:
2158 1846 1831 1717 321
ApoI r'AATT_y
Cuts at: 1224 2988 4875 7226 7482 1224
Size: 1764 1887 2351 256 1615
Fragments arranged by size:
2351 1887 1764 1615 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7873
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7873
AvaI C'yCGr_G
Cuts at: 209 224 1938 2899 2933 3534 4274 4891 209
Size: 15 1714 961 34 601 740 617 3191
Fragments arranged by size:
3191 1714 961 740 617 601 34 15
AvrII C'CTAG_G
Cuts at: 3847 3847
Size: 7873
BamHI G'GATC_C
Cuts at: 3921 4896 3921
Size: 975 6898
BanI G'GyrC_C
Cuts at: 1751 1767 2181 2688 3487 3813 4887 5301 5549
Size: 16 414 507 799 326 1074 414 248
Cuts at: 5549 5584 1751
Size: 35 4040
Fragments arranged by size:
4040 1074 799 507 414 414 326 248
35 16
BanII G_rGCy'C
Cuts at: 254 1843 2325 2486 2744 2900 3257 3269 4281
Size: 1589 482 161 258 156 357 12 1012
Cuts at: 4281 4522 4885 5915 254
Size: 241 363 1030 2212
Fragments arranged by size:
2212 1589 1030 1012 482 363 357 258
241 161 156 12
BbeI G_GCGC'C
Cuts at: 1755 5305 5553 1755
Size: 3550 248 4075
Fragments arranged by size:
4075 3550 248
BbsI GAAGACnn'nnnn_
Cuts at: 458 589 956 2143 2589 2933 4193 458
Size: 131 367 1187 446 344 1260 4138
Fragments arranged by size:
4138 1260 1187 446 367 344 131
BbvCI CC'TCA_GC
Cuts at: 3018 3018
Size: 7873
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 772 806 2140 2174 4286 4320 772
Size: 34 1334 34 2112 34 4325
Fragments arranged by size:
4325 2112 1334 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5769 6532 7873 5769
Size: 763 1341 5769
Fragments arranged by size:
5769 1341 763
BclI T'GATC_A
Cuts at: 417 4553 5391 417
Size: 4136 838 2899
Fragments arranged by size:
4136 2899 838
BglI GCCn_nnn'nGGC
Cuts at: 2571 3205 4266 4810 2571
Size: 634 1061 544 5634
Fragments arranged by size:
5634 1061 634 544
BglII A'GATC_T
Cuts at: 1100 5386 7425 1100
Size: 4286 2039 1548
Fragments arranged by size:
4286 2039 1548
BlpI GC'TnA_GC
Cuts at: 4983 4983
Size: 7873
Bme1580I G_kGCm'C
Cuts at: 254 605 1089 1843 2184 2486 2763 4347 4480
Size: 351 484 754 341 302 277 1584 133
Cuts at: 4480 4801 5496 5589 6647 254
Size: 321 695 93 1058 1480
Fragments arranged by size:
1584 1480 1058 754 695 484 351 341
321 302 277 133 93
BmgBI CAC'GTC
Cuts at: 530 2721 530
Size: 2191 5682
BmrI ACTGGGnnnn_n'
Cuts at: 1137 1603 2121 2360 2504 5083 5164 5499 7645
Size: 466 518 239 144 2579 81 335 2146
Cuts at: 7645 1137
Size: 1365
Fragments arranged by size:
2579 2146 1365 518 466 335 239 144
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1728 4353 6238 1728
Size: 2625 1885 3363
Fragments arranged by size:
3363 2625 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 307 985 1974 2725 2836 2862 3156 3241
Size: 150 678 989 751 111 26 294 85
Cuts at: 3241 3566 3776 4284 4323 4435 157
Size: 325 210 508 39 112 3595
Fragments arranged by size:
3595 989 751 678 508 325 294 210
150 112 111 85 39 26
Bpu10I CC'TnA_GC
Cuts at: 3018 3071 3881 3018
Size: 53 810 7010
Fragments arranged by size:
7010 810 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1288 3089 3221 3653 3695 5047 5926 6420
Size: 630 1801 132 432 42 1352 879 494
Cuts at: 6420 6718 658
Size: 298 1813
Fragments arranged by size:
1813 1801 1352 879 630 494 432 298
132 42
BsaI GGTCTCn'nnnn_
Cuts at: 133 2679 2812 2848 2896 3656 7577 133
Size: 2546 133 36 48 760 3921 429
Fragments arranged by size:
3921 2546 760 429 133 48 36
BsaAI yAC'GTr
Cuts at: 4863 5224 4863
Size: 361 7512
BsaBI GATnn'nnATC
Cuts at: 6 3294 5385 7424 6
Size: 3288 2091 2039 455
Fragments arranged by size:
3288 2091 2039 455
BsaHI Gr'CG_yC
Cuts at: 1520 1752 1891 5302 5550 6254 7750 1520
Size: 232 139 3411 248 704 1496 1643
Fragments arranged by size:
3411 1643 1496 704 248 232 139
BsaWI w'CCGG_w
Cuts at: 2963 5060 5581 6261 6535 6682 7093 2963
Size: 2097 521 680 274 147 411 3743
Fragments arranged by size:
3743 2097 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 751 781 805 835 1969 1999 2860 2890 3239
Size: 30 24 30 1134 30 861 30 349
Cuts at: 3239 3269 751
Size: 30 5355
Fragments arranged by size:
5355 1134 861 349 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 577 892 1720 1807 1977 2074 2834 3006
Size: 267 315 828 87 170 97 760 172
Cuts at: 3006 3020 3520 310
Size: 14 500 4663
Fragments arranged by size:
4663 828 760 500 315 267 172 170
97 87 14
BseYI C'CCAG_C
Cuts at: 1088 1265 1582 1843 2177 2360 2871 5296 6054
Size: 177 317 261 334 183 511 2425 758
Cuts at: 6054 6633 7129 1088
Size: 579 496 1832
Fragments arranged by size:
2425 1832 758 579 511 496 334 317
261 183 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 814 823 1022 1042 2377 4146 4272 510
Size: 304 9 199 20 1335 1769 126 4111
Fragments arranged by size:
4111 1769 1335 304 199 126 20 9
BsiEI CG_ry'CG
Cuts at: 104 4814 5413 5459 6669 104
Size: 4710 599 46 1210 1308
Fragments arranged by size:
4710 1308 1210 599 46
BsiHKAI G_wGCw'C
Cuts at: 605 623 674 1001 2197 2763 2866 3257 4480
Size: 18 51 327 1196 566 103 391 1223
Cuts at: 4480 4801 4885 5663 5853 6647 605
Size: 321 84 778 190 794 1831
Fragments arranged by size:
1831 1223 1196 794 778 566 391 327
321 190 103 84 51 18
Bsp1286I G_dGCh'C
Cuts at: 254 605 623 674 1001 1089 1843 2184 2197
Size: 351 18 51 327 88 754 341 13
Cuts at: 2197 2325 2486 2744 2763 2866 2900 3257 3269
Size: 128 161 258 19 103 34 357 12
Cuts at: 3269 4281 4347 4480 4522 4801 4885 5496 5589
Size: 1012 66 133 42 279 84 611 93
Cuts at: 5589 5663 5853 5915 6647 254
Size: 74 190 62 732 1480
Fragments arranged by size:
1480 1012 754 732 611 357 351 341
327 279 258 190 161 133 128 103
93 88 84 74 66 62 51 42
34 19 18 13 12
BspEI T'CCGG_A
Cuts at: 2963 7093 2963
Size: 4130 3743
BspHI T'CATG_A
Cuts at: 7049 7049
Size: 7873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 842 852 1858 1985 1990 4756 4921 5135 5437
Size: 10 1006 127 5 2766 165 214 302
Cuts at: 5437 5818 842
Size: 381 2897
Fragments arranged by size:
2897 2766 1006 381 302 214 165 127
10 5
BsrBI CCG'CTC
Cuts at: 50 6163 6217 7703 7868 50
Size: 6113 54 1486 165 55
Fragments arranged by size:
6113 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 3945 5783 1364
Size: 2581 1838 3454
Fragments arranged by size:
3454 2581 1838
BsrFI r'CCGG_y
Cuts at: 4627 5869 6050 6261 4627
Size: 1242 181 211 6239
Fragments arranged by size:
6239 1242 211 181
BsrGI T'GTAC_A
Cuts at: 3304 4709 3304
Size: 1405 6468
BssSI C'ACGA_G
Cuts at: 6142 6502 6142
Size: 360 7513
BstAPI GCAn_nnn'nTGC
Cuts at: 837 1447 2793 5248 837
Size: 610 1346 2455 3462
Fragments arranged by size:
3462 2455 1346 610
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7873
BstEII G'GTnAC_C
Cuts at: 3178 3178
Size: 7873
BstXI CCAn_nnnn'nTGG
Cuts at: 331 2829 2886 4071 331
Size: 2498 57 1185 4133
Fragments arranged by size:
4133 2498 1185 57
BstYI r'GATC_y
Cuts at: 1 422 1073 1100 1289 3641 3921 4896 5363
Size: 421 651 27 189 2352 280 975 467
Cuts at: 5363 5386 5721 5967 6981 7067 7079 7425 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
2352 1014 975 651 467 449 421 346
335 280 246 189 86 27 23 12
Bsu36I CC'TnA_GG
Cuts at: 1823 2629 4382 4696 1823
Size: 806 1753 314 5000
Fragments arranged by size:
5000 1753 806 314
BtgI C'CryG_G
Cuts at: 125 956 1917 1949 2906 4158 4211 4850 125
Size: 831 961 32 957 1252 53 639 3148
Fragments arranged by size:
3148 1252 961 957 831 639 53 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1007 5821 6002 6165 1007
Size: 4814 181 163 2715
Fragments arranged by size:
4814 2715 181 163
BtsI GCAGTG_nn'
Cuts at: 1701 5114 1701
Size: 3413 4460
DraI TTT'AAA
Cuts at: 1109 1277 4870 1109
Size: 168 3593 4112
Fragments arranged by size:
4112 3593 168
DrdI GACnn_nn'nnGTC
Cuts at: 5577 6437 5577
Size: 860 7013
EaeI y'GGCC_r
Cuts at: 2270 3206 4061 4324 4625 4811 5456 5630 6021
Size: 936 855 263 301 186 645 174 391
Cuts at: 6021 6048 7395 2270
Size: 27 1347 2748
Fragments arranged by size:
2748 1347 936 855 645 391 301 263
186 174 27
EagI C'GGCC_G
Cuts at: 4811 5456 4811
Size: 645 7228
EarI CTCTTCn'nnn_
Cuts at: 125 562 800 839 907 1207 1472 1958 3425
Size: 437 238 39 68 300 265 486 1467
Cuts at: 3425 4571 5894 6104 125
Size: 1146 1323 210 1894
Fragments arranged by size:
1894 1467 1323 1146 486 437 300 265
238 210 68 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4656 6079 6391 6537 7683 4656
Size: 1423 312 146 1146 4846
Fragments arranged by size:
4846 1423 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3255 4883 3255
Size: 1628 6245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 307 470 601 632 888 985 1498 1974
Size: 150 163 131 31 256 97 513 476
Cuts at: 1974 2725 2836 2862 3156 3241 3566 3776 4058
Size: 751 111 26 294 85 325 210 282
Cuts at: 4058 4284 4323 4435 5696 6128 6877 7145 157
Size: 226 39 112 1261 432 749 268 885
Fragments arranged by size:
1261 885 751 749 513 476 432 325
294 282 268 256 226 210 163 150
131 112 111 97 85 39 31 26
EcoNI CCTnn'n_nnAGG
Cuts at: 322 559 1992 3626 322
Size: 237 1433 1634 4569
Fragments arranged by size:
4569 1634 1433 237
EcoO109I rG'GnC_Cy
Cuts at: 927 1688 1839 1840 2470 2482 5010 927
Size: 761 151 1 630 12 2528 3790
Fragments arranged by size:
3790 2528 761 630 151 12 1
EcoRI G'AATT_C
Cuts at: 2988 4875 2988
Size: 1887 5986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 621 699 1528 2660 5890 520
Size: 101 78 829 1132 3230 2503
Fragments arranged by size:
3230 2503 1132 829 101 78
FseI GG_CCGG'CC
Cuts at: 4631 4631
Size: 7873
FspI TGC'GCA
Cuts at: 5652 5652
Size: 7873
HaeII r_GCGC'y
Cuts at: 207 1011 1755 5305 5553 6577 7556 207
Size: 804 744 3550 248 1024 979 524
Fragments arranged by size:
3550 1024 979 804 744 524 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 1342 1374 1969 2001 2407 2439 2650
Size: 32 559 32 595 32 406 32 211
Cuts at: 2650 2682 3562 3594 4086 4118 5713 5743 5745
Size: 32 880 32 492 32 1595 30 2
Cuts at: 5745 5775 751
Size: 30 2849
Fragments arranged by size:
2849 1595 880 595 559 492 406 211
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1605 4910 1605
Size: 3305 4568
HindIII A'AGCT_T
Cuts at: 403 704 2520 403
Size: 301 1816 5756
Fragments arranged by size:
5756 1816 301
KasI G'GCGC_C
Cuts at: 1751 5301 5549 1751
Size: 3550 248 4075
Fragments arranged by size:
4075 3550 248
KpnI G_GTAC'C
Cuts at: 4891 4891
Size: 7873
MluI A'CGCG_T
Cuts at: 7306 7306
Size: 7873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1068 1177 1359 1574 1919 3242 3260 3716 4419
Size: 109 182 215 345 1323 18 456 703
Cuts at: 4419 4431 6544 6728 1068
Size: 12 2113 184 2213
Fragments arranged by size:
2213 2113 1323 703 456 345 215 184
182 109 18 12
MscI TGG'CCA
Cuts at: 3208 4063 4326 5632 3208
Size: 855 263 1306 5449
Fragments arranged by size:
5449 1306 855 263
MslI CAynn'nnrTG
Cuts at: 283 730 2890 3906 5860 5987 283
Size: 447 2160 1016 1954 127 2169
Fragments arranged by size:
2169 2160 1954 1016 447 127
MspA1I CmG'CkG
Cuts at: 244 896 1919 3586 4130 4286 4356 4623 4661
Size: 652 1023 1667 544 156 70 267 38
Cuts at: 4661 4687 4982 5296 5656 6671 6916 244
Size: 26 295 314 360 1015 245 1201
Fragments arranged by size:
1667 1201 1023 1015 652 544 360 314
295 267 245 156 70 38 26
NaeI GCC'GGC
Cuts at: 4629 6052 4629
Size: 1423 6450
NarI GG'CG_CC
Cuts at: 1752 5302 5550 1752
Size: 3550 248 4075
Fragments arranged by size:
4075 3550 248
NcoI C'CATG_G
Cuts at: 125 1949 2906 4211 125
Size: 1824 957 1305 3787
Fragments arranged by size:
3787 1824 1305 957
NdeI CA'TA_TG
Cuts at: 1311 1311
Size: 7873
NgoMIV G'CCGG_C
Cuts at: 4627 6050 4627
Size: 1423 6450
NruI TCG'CGA
Cuts at: 6234 6234
Size: 7873
NspI r_CATG'y
Cuts at: 2796 4154 4552 4924 5424 6333 2796
Size: 1358 398 372 500 909 4336
Fragments arranged by size:
4336 1358 909 500 398 372
PasI CC'CwG_GG
Cuts at: 3691 3691
Size: 7873
PciI A'CATG_T
Cuts at: 6329 6329
Size: 7873
PflMI CCAn_nnn'nTGG
Cuts at: 864 4401 4614 864
Size: 3537 213 4123
Fragments arranged by size:
4123 3537 213
PfoI T'CCnGG_A
Cuts at: 323 1001 1064 2099 3396 323
Size: 678 63 1035 1297 4800
Fragments arranged by size:
4800 1297 1035 678 63
PmeI GTTT'AAAC
Cuts at: 4870 4870
Size: 7873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2274 2306 7012 7044 2274
Size: 32 4706 32 3103
Fragments arranged by size:
4706 3103 32 32
PpuMI rG'GwC_Cy
Cuts at: 927 927
Size: 7873
PspOMI G'GGCC_C
Cuts at: 250 1839 2482 250
Size: 1589 643 5641
Fragments arranged by size:
5641 1589 643
PspXI vC'TCGA_Gb
Cuts at: 1938 1938
Size: 7873
PstI C_TGCA'G
Cuts at: 243 983 991 1668 1696 1777 2505 2789 4918
Size: 740 8 677 28 81 728 284 2129
Cuts at: 4918 5603 243
Size: 685 2513
Fragments arranged by size:
2513 2129 740 728 685 677 284 81
28 8
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7873
PvuII CAG'CTG
Cuts at: 244 3586 4130 4286 4356 4623 5296 5656 244
Size: 3342 544 156 70 267 673 360 2461
Fragments arranged by size:
3342 2461 673 544 360 267 156 70
RsrII CG'GwC_CG
Cuts at: 6066 6066
Size: 7873
SacI G_AGCT'C
Cuts at: 3257 4885 3257
Size: 1628 6245
SacII CC_GC'GG
Cuts at: 1920 1920
Size: 7873
SalI G'TCGA_C
Cuts at: 4908 4908
Size: 7873
SapI GCTCTTCn'nnn_
Cuts at: 125 800 3425 5894 6104 125
Size: 675 2625 2469 210 1894
Fragments arranged by size:
2625 2469 1894 675 210
SbfI CC_TGCA'GG
Cuts at: 4918 4918
Size: 7873
ScaI AGT'ACT
Cuts at: 787 4333 7390 787
Size: 3546 3057 1270
Fragments arranged by size:
3546 3057 1270
SexAI A'CCwGG_T
Cuts at: 4613 4613
Size: 7873
SfcI C'TryA_G
Cuts at: 33 239 979 987 1016 1285 1590 1664 1692
Size: 206 740 8 29 269 305 74 28
Cuts at: 1692 1773 1923 2244 2501 2650 2785 4653 4914
Size: 81 150 321 257 149 135 1868 261
Cuts at: 4914 5599 6594 6785 33
Size: 685 995 191 1121
Fragments arranged by size:
1868 1121 995 740 685 321 305 269
261 257 206 191 150 149 135 81
74 29 28 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 4810 4810
Size: 7873
SfoI GGC'GCC
Cuts at: 1753 5303 5551 1753
Size: 3550 248 4075
Fragments arranged by size:
4075 3550 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7873
SmaI CCC'GGG
Cuts at: 211 226 3536 4893 211
Size: 15 3310 1357 3191
Fragments arranged by size:
3310 3191 1357 15
SmlI C'TyrA_G
Cuts at: 673 1303 1938 2933 3104 3236 3668 3710 5026
Size: 630 635 995 171 132 432 42 1316
Cuts at: 5026 5941 6435 6697 673
Size: 915 494 262 1849
Fragments arranged by size:
1849 1316 995 915 635 630 494 432
262 171 132 42
SnaBI TAC'GTA
Cuts at: 4863 4863
Size: 7873
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7873
SphI G_CATG'C
Cuts at: 4924 5424 4924
Size: 500 7373
SrfI GCCC'GGGC
Cuts at: 226 226
Size: 7873
StuI AGG'CCT
Cuts at: 2027 2827 3081 4269 2027
Size: 800 254 1188 5631
Fragments arranged by size:
5631 1188 800 254
StyI C'CwwG_G
Cuts at: 125 923 1949 2754 2822 2828 2906 3847 4211
Size: 798 1026 805 68 6 78 941 364
Cuts at: 4211 5005 5359 125
Size: 794 354 2639
Fragments arranged by size:
2639 1026 941 805 798 794 364 354
78 68 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 2411 2671 3649 5454 5587 6046 6257 574
Size: 1837 260 978 1805 133 459 211 2190
Fragments arranged by size:
2190 1837 1805 978 459 260 211 133
TatI w'GTAC_w
Cuts at: 785 812 2299 3304 4331 4709 7388 785
Size: 27 1487 1005 1027 378 2679 1270
Fragments arranged by size:
2679 1487 1270 1027 1005 378 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3770 3802 4235 4267 7390 7422 3239
Size: 32 499 32 433 32 3123 32 3690
Fragments arranged by size:
3690 3123 499 433 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5668 5668
Size: 7873
XbaI T'CTAG_A
Cuts at: 4902 4902
Size: 7873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 930 1824 1956 2373 4056 4113 5366 132
Size: 798 894 132 417 1683 57 1253 2639
Fragments arranged by size:
2639 1683 1253 894 798 417 132 57
XhoI C'TCGA_G
Cuts at: 1938 2933 1938
Size: 995 6878
XmaI C'CCGG_G
Cuts at: 209 224 3534 4891 209
Size: 15 3310 1357 3191
Fragments arranged by size:
3310 3191 1357 15
ZraI GAC'GTC
Cuts at: 1521 1521
Size: 7873
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI BaeI BfrBI BmtI BsiWI
BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FspAI HpaI MfeI NheI NotI NsiI PacI PmlI
PshAI PsiI PsrI SanDI SgrAI SspI SwaI XmnI