Restriction map for
pF1KSDA0975
(Circular) (Six-base) MAPSORT of: pF1KSDA0975.seq Check: 7777 from: 1 to: 7649
pF1KSDA0975 7649 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1906 3873 4524 1906
Size: 1967 651 5031
Fragments arranged by size:
5031 1967 651
AccI GT'mk_AC
Cuts at: 407 2699 2708 3116 4687 407
Size: 2292 9 408 1571 3369
Fragments arranged by size:
3369 2292 1571 408 9
Acc65I G'GTAC_C
Cuts at: 3056 4337 4665 3056
Size: 1281 328 6040
Fragments arranged by size:
6040 1281 328
AclI AA'CG_TT
Cuts at: 7489 7489
Size: 7649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 926 956 3169 3356 5474 5906 6653 6921
Size: 390 30 2213 187 2118 432 747 268
Cuts at: 6921 536
Size: 1264
Fragments arranged by size:
2213 2118 1264 747 432 390 268 187
30
AflII C'TTAA_G
Cuts at: 685 694 685
Size: 9 7640
AflIII A'CryG_T
Cuts at: 1343 5001 6105 7082 1343
Size: 3658 1104 977 1910
Fragments arranged by size:
3658 1910 1104 977
AgeI A'CCGG_T
Cuts at: 6037 6037
Size: 7649
AhdI GACnn_n'nnGTC
Cuts at: 3822 3822
Size: 7649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 605 1997 3518 605
Size: 1392 1521 4736
Fragments arranged by size:
4736 1521 1392
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4096 4128 4096
Size: 32 7617
AlwNI CAG_nnn'CTG
Cuts at: 546 750 1116 1813 1941 3147 3313 4954 6521
Size: 204 366 697 128 1206 166 1641 1567
Cuts at: 6521 546
Size: 1674
Fragments arranged by size:
1674 1641 1567 1206 697 366 204 166
128
ApaI G_GGCC'C
Cuts at: 152 2102 2205 4225 152
Size: 1950 103 2020 3576
Fragments arranged by size:
3576 2020 1950 103
ApaLI G'TGCA_C
Cuts at: 6419 6419
Size: 7649
ApoI r'AATT_y
Cuts at: 916 4653 7002 7258 916
Size: 3737 2349 256 1307
Fragments arranged by size:
3737 2349 1307 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7649
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7649
AvaI C'yCGr_G
Cuts at: 151 655 1717 2363 3011 3175 3444 4303 4669
Size: 504 1062 646 648 164 269 859 366
Cuts at: 4669 151
Size: 3131
Fragments arranged by size:
3131 1062 859 648 646 504 366 269
164
BamHI G'GATC_C
Cuts at: 4674 4674
Size: 7649
BanI G'GyrC_C
Cuts at: 1860 2917 3016 3056 3602 4337 4485 4665 5079
Size: 1057 99 40 546 735 148 180 414
Cuts at: 5079 5327 5362 1860
Size: 248 35 4147
Fragments arranged by size:
4147 1057 735 546 414 248 180 148
99 40 35
BanII G_rGCy'C
Cuts at: 152 196 524 2102 2205 3202 3560 4225 4631
Size: 44 328 1578 103 997 358 665 406
Cuts at: 4631 4663 5693 152
Size: 32 1030 2108
Fragments arranged by size:
2108 1578 1030 997 665 406 358 328
103 44 32
BbeI G_GCGC'C
Cuts at: 3020 5083 5331 3020
Size: 2063 248 5338
Fragments arranged by size:
5338 2063 248
BbsI GAAGACnn'nnnn_
Cuts at: 1556 2331 3157 3349 4416 1556
Size: 775 826 192 1067 4789
Fragments arranged by size:
4789 1067 826 775 192
BbvCI CC'TCA_GC
Cuts at: 3324 3578 3598 4159 3324
Size: 254 20 561 6814
Fragments arranged by size:
6814 561 254 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 547 581 3415 3449 547
Size: 34 2834 34 4747
Fragments arranged by size:
4747 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2299 5547 6308 7649 2299
Size: 3248 761 1341 2299
Fragments arranged by size:
3248 2299 1341 761
BclI T'GATC_A
Cuts at: 5169 5169
Size: 7649
BglI GCCn_nnn'nGGC
Cuts at: 169 3539 169
Size: 3370 4279
BglII A'GATC_T
Cuts at: 4262 5164 7201 4262
Size: 902 2037 4710
Fragments arranged by size:
4710 2037 902
BlpI GC'TnA_GC
Cuts at: 812 2953 4136 4761 812
Size: 2141 1183 625 3700
Fragments arranged by size:
3700 2141 1183 625
Bme1580I G_kGCm'C
Cuts at: 152 662 2102 2205 4225 5274 5367 6423 152
Size: 510 1440 103 2020 1049 93 1056 1378
Fragments arranged by size:
2020 1440 1378 1056 1049 510 103 93
BmgBI CAC'GTC
Cuts at: 1803 2070 2109 3525 4434 1803
Size: 267 39 1416 909 5018
Fragments arranged by size:
5018 1416 909 267 39
BmrI ACTGGGnnnn_n'
Cuts at: 3283 3432 3786 4079 4541 4861 4942 5277 7421
Size: 149 354 293 462 320 81 335 2144
Cuts at: 7421 3283
Size: 3511
Fragments arranged by size:
3511 2144 462 354 335 320 293 149
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 559 1437 4585 6016 559
Size: 878 3148 1431 2192
Fragments arranged by size:
3148 2192 1431 878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 421 1357 2251 3290 3392 3667 3916 397
Size: 24 936 894 1039 102 275 249 4130
Fragments arranged by size:
4130 1039 936 894 275 249 102 24
Bpu10I CC'TnA_GC
Cuts at: 849 1006 1942 3324 3578 3598 4159 849
Size: 157 936 1382 254 20 561 4339
Fragments arranged by size:
4339 1382 936 561 254 157 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 2233 2455 4825 5704 6196 6494 351
Size: 1882 222 2370 879 492 298 1506
Fragments arranged by size:
2370 1882 1506 879 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 7353 7353
Size: 7649
BsaAI yAC'GTr
Cuts at: 626 1344 3036 3893 4176 4641 5002 626
Size: 718 1692 857 283 465 361 3273
Fragments arranged by size:
3273 1692 857 718 465 361 283
BsaBI GATnn'nnATC
Cuts at: 6 5163 7200 6
Size: 5157 2037 455
Fragments arranged by size:
5157 2037 455
BsaHI Gr'CG_yC
Cuts at: 2513 3017 3394 5080 5328 6030 7526 2513
Size: 504 377 1686 248 702 1496 2636
Fragments arranged by size:
2636 1686 1496 702 504 377 248
BsaWI w'CCGG_w
Cuts at: 1310 1385 4838 5359 6037 6311 6458 6869 1310
Size: 75 3453 521 678 274 147 411 2090
Fragments arranged by size:
3453 2090 678 521 411 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1006 1036 1141 1171 1330 1360 1498 1528 1593
Size: 30 105 30 159 30 138 30 65
Cuts at: 1593 1623 2371 2401 3988 4018 4588 4618 1006
Size: 30 748 30 1587 30 570 30 4037
Fragments arranged by size:
4037 1587 748 570 159 138 105 65
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1149 1318 1687 2026 2050 2053 2062 2065 2077
Size: 169 369 339 24 3 9 3 12
Cuts at: 2077 2128 2131 2134 2141 2161 2164 2173 2191
Size: 51 3 3 7 20 3 9 18
Cuts at: 2191 2194 2379 3403 3490 3607 4105 4108 1149
Size: 3 185 1024 87 117 498 3 4690
Fragments arranged by size:
4690 1024 498 369 339 185 169 117
87 51 24 20 18 12 9 9
7 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1185 1585 1631 2564 2867 3005 3264 3300 3336
Size: 400 46 933 303 138 259 36 36
Cuts at: 3336 3354 3420 4152 5074 5832 6409 6905 1185
Size: 18 66 732 922 758 577 496 1929
Fragments arranged by size:
1929 933 922 758 732 577 496 400
303 259 138 66 46 36 36 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2299 2509 2749 2760 4198 1228
Size: 1071 210 240 11 1438 4679
Fragments arranged by size:
4679 1438 1071 240 210 11
BsiEI CG_ry'CG
Cuts at: 104 134 2738 5191 5237 6445 104
Size: 30 2604 2453 46 1208 1308
Fragments arranged by size:
2604 2453 1308 1208 46 30
BsiHKAI G_wGCw'C
Cuts at: 524 1343 1724 1948 2237 2318 2471 3455 3560
Size: 819 381 224 289 81 153 984 105
Cuts at: 3560 3698 4385 4631 4663 5441 5631 6423 524
Size: 138 687 246 32 778 190 792 1750
Fragments arranged by size:
1750 984 819 792 778 687 381 289
246 224 190 153 138 105 81 32
BsmBI CGTCTCn'nnnn_
Cuts at: 3385 3385
Size: 7649
Bsp1286I G_dGCh'C
Cuts at: 152 196 524 662 1343 1724 1948 2102 2205
Size: 44 328 138 681 381 224 154 103
Cuts at: 2205 2237 2318 2471 3202 3455 3560 3698 4225
Size: 32 81 153 731 253 105 138 527
Cuts at: 4225 4385 4631 4663 5274 5367 5441 5631 5693
Size: 160 246 32 611 93 74 190 62
Cuts at: 5693 6423 152
Size: 730 1378
Fragments arranged by size:
1378 731 730 681 611 527 381 328
253 246 224 190 160 154 153 138
138 105 103 93 81 74 62 44
32 32
BspEI T'CCGG_A
Cuts at: 1310 6869 1310
Size: 5559 2090
BspHI T'CATG_A
Cuts at: 4443 6825 4443
Size: 2382 5267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 341 1906 2531 2552 3827 3873 4524 4699
Size: 42 1565 625 21 1275 46 651 175
Cuts at: 4699 4913 5215 5596 299
Size: 214 302 381 2352
Fragments arranged by size:
2352 1565 1275 651 625 381 302 214
175 46 42 21
BsrBI CCG'CTC
Cuts at: 50 156 1971 2967 5941 5995 7479 7644 50
Size: 106 1815 996 2974 54 1484 165 55
Fragments arranged by size:
2974 1815 1484 996 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 3246 3572 5561 1730
Size: 1516 326 1989 3818
Fragments arranged by size:
3818 1989 1516 326
BsrFI r'CCGG_y
Cuts at: 168 553 4340 4633 5647 5828 6037 168
Size: 385 3787 293 1014 181 209 1780
Fragments arranged by size:
3787 1780 1014 385 293 209 181
BssHII G'CGCG_C
Cuts at: 136 181 2810 136
Size: 45 2629 4975
Fragments arranged by size:
4975 2629 45
BssSI C'ACGA_G
Cuts at: 2527 3049 3091 5920 6278 2527
Size: 522 42 2829 358 3898
Fragments arranged by size:
3898 2829 522 358 42
BstAPI GCAn_nnn'nTGC
Cuts at: 750 5026 750
Size: 4276 3373
BstBI TT'CG_AA
Cuts at: 110 6010 110
Size: 5900 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 456 1398 456
Size: 942 6707
BstYI r'GATC_y
Cuts at: 1 396 1272 2345 3485 4262 4674 5141 5164
Size: 395 876 1073 1140 777 412 467 23
Cuts at: 5164 5499 5745 6757 6843 6855 7201 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1140 1073 1012 876 777 467 449 412
395 346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3330 3330
Size: 7649
BtgI C'CryG_G
Cuts at: 125 503 2913 125
Size: 378 2410 4861
Fragments arranged by size:
4861 2410 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2316 3147 5599 5780 5943 2316
Size: 831 2452 181 163 4022
Fragments arranged by size:
4022 2452 831 181 163
BtsI GCAGTG_nn'
Cuts at: 3068 3730 3921 4297 4464 4892 3068
Size: 662 191 376 167 428 5825
Fragments arranged by size:
5825 662 428 376 191 167
DraI TTT'AAA
Cuts at: 4648 4648
Size: 7649
DrdI GACnn_nn'nnGTC
Cuts at: 5355 6213 5355
Size: 858 6791
EaeI y'GGCC_r
Cuts at: 170 1682 1872 1913 2573 2735 3030 3213 4353
Size: 1512 190 41 660 162 295 183 1140
Cuts at: 4353 4608 5234 5408 5799 5826 7171 170
Size: 255 626 174 391 27 1345 648
Fragments arranged by size:
1512 1345 1140 660 648 626 391 295
255 190 183 174 162 41 27
EagI C'GGCC_G
Cuts at: 2735 5234 2735
Size: 2499 5150
EarI CTCTTCn'nnn_
Cuts at: 379 512 2054 2138 2150 2162 2503 3565 5672
Size: 133 1542 84 12 12 341 1062 2107
Cuts at: 5672 5882 379
Size: 210 2146
Fragments arranged by size:
2146 2107 1542 1062 341 210 133 84
12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 329 2281 2823 3700 5857 6167 6313 7459 329
Size: 1952 542 877 2157 310 146 1146 519
Fragments arranged by size:
2157 1952 1146 877 542 519 310 146
EcoICRI GAG'CTC
Cuts at: 522 3558 4629 4661 522
Size: 3036 1071 32 3510
Fragments arranged by size:
3510 3036 1071 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 421 536 926 956 1357 2251 3169 3290
Size: 24 115 390 30 401 894 918 121
Cuts at: 3290 3356 3392 3667 3916 5474 5906 6653 6921
Size: 66 36 275 249 1558 432 747 268
Cuts at: 6921 397
Size: 1125
Fragments arranged by size:
1558 1125 918 894 747 432 401 390
275 268 249 121 115 66 36 30
24
EcoNI CCTnn'n_nnAGG
Cuts at: 784 3460 4276 784
Size: 2676 816 4157
Fragments arranged by size:
4157 2676 816
EcoO109I rG'GnC_Cy
Cuts at: 715 955 1418 1677 2098 2099 2202 3144 3261
Size: 240 463 259 421 1 103 942 117
Cuts at: 3261 3429 4104 4149 4221 4222 4368 4410 4599
Size: 168 675 45 72 1 146 42 189
Cuts at: 4599 4788 715
Size: 189 3576
Fragments arranged by size:
3576 942 675 463 421 259 240 189
189 168 146 117 103 72 45 42
1 1
EcoRI G'AATT_C
Cuts at: 4653 4653
Size: 7649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 864 2910 3120 5668 864
Size: 2046 210 2548 2845
Fragments arranged by size:
2845 2548 2046 210
FspI TGC'GCA
Cuts at: 5430 5430
Size: 7649
HaeII r_GCGC'y
Cuts at: 1941 2187 2802 3020 3194 3548 5083 5331 6353
Size: 246 615 218 174 354 1535 248 1022
Cuts at: 6353 7332 1941
Size: 979 2258
Fragments arranged by size:
2258 1535 1022 979 615 354 248 246
218 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 815 847 1330 1362 1498 1530 2533 2565 3808
Size: 32 483 32 136 32 1003 32 1243
Cuts at: 3808 3840 3988 4020 5491 5521 5523 5553 815
Size: 32 148 32 1471 30 2 30 2911
Fragments arranged by size:
2911 1471 1243 1003 483 148 136 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1266 3990 4688 1266
Size: 2724 698 4227
Fragments arranged by size:
4227 2724 698
HindIII A'AGCT_T
Cuts at: 372 3808 372
Size: 3436 4213
KasI G'GCGC_C
Cuts at: 3016 5079 5327 3016
Size: 2063 248 5338
Fragments arranged by size:
5338 2063 248
KpnI G_GTAC'C
Cuts at: 3060 4341 4669 3060
Size: 1281 328 6040
Fragments arranged by size:
6040 1281 328
MluI A'CGCG_T
Cuts at: 7082 7082
Size: 7649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6320 6504 6320
Size: 184 7465
MscI TGG'CCA
Cuts at: 1684 1874 2575 3032 3215 5410 1684
Size: 190 701 457 183 2195 3923
Fragments arranged by size:
3923 2195 701 457 190 183
MslI CAynn'nnrTG
Cuts at: 482 806 2575 3079 3877 3946 5638 5765 482
Size: 324 1769 504 798 69 1692 127 2366
Fragments arranged by size:
2366 1769 1692 798 504 324 127 69
MspA1I CmG'CkG
Cuts at: 505 585 606 1567 1977 2007 2637 2774 2999
Size: 80 21 961 410 30 630 137 225
Cuts at: 2999 3126 4760 5074 5434 6447 6692 505
Size: 127 1634 314 360 1013 245 1462
Fragments arranged by size:
1634 1462 1013 961 630 410 360 314
245 225 137 127 80 30 21
NaeI GCC'GGC
Cuts at: 170 555 5830 170
Size: 385 5275 1989
Fragments arranged by size:
5275 1989 385
NarI GG'CG_CC
Cuts at: 3017 5080 5328 3017
Size: 2063 248 5338
Fragments arranged by size:
5338 2063 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 7649
NgoMIV G'CCGG_C
Cuts at: 168 553 5828 168
Size: 385 5275 1989
Fragments arranged by size:
5275 1989 385
NspI r_CATG'y
Cuts at: 1840 4200 4702 5202 6109 1840
Size: 2360 502 500 907 3380
Fragments arranged by size:
3380 2360 907 502 500
PasI CC'CwG_GG
Cuts at: 1737 2205 3195 3640 4207 1737
Size: 468 990 445 567 5179
Fragments arranged by size:
5179 990 567 468 445
PciI A'CATG_T
Cuts at: 6105 6105
Size: 7649
PflMI CCAn_nnn'nTGG
Cuts at: 2571 4131 4207 2571
Size: 1560 76 6013
Fragments arranged by size:
6013 1560 76
PfoI T'CCnGG_A
Cuts at: 665 1067 1277 4271 4364 665
Size: 402 210 2994 93 3950
Fragments arranged by size:
3950 2994 402 210 93
PmeI GTTT'AAAC
Cuts at: 4648 4648
Size: 7649
PmlI CAC'GTG
Cuts at: 626 1344 3036 3893 626
Size: 718 1692 857 4382
Fragments arranged by size:
4382 1692 857 718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6788 6820 6788
Size: 32 7617
PpuMI rG'GwC_Cy
Cuts at: 715 3144 3261 4104 4368 715
Size: 2429 117 843 264 3996
Fragments arranged by size:
3996 2429 843 264 117
PshAI GACnn'nnGTC
Cuts at: 967 1076 3157 3248 967
Size: 109 2081 91 5368
Fragments arranged by size:
5368 2081 109 91
PspOMI G'GGCC_C
Cuts at: 148 2098 2201 4221 148
Size: 1950 103 2020 3576
Fragments arranged by size:
3576 2020 1950 103
PstI C_TGCA'G
Cuts at: 584 611 1112 2621 3143 3318 3737 3824 4219
Size: 27 501 1509 522 175 419 87 395
Cuts at: 4219 4696 5381 584
Size: 477 685 2852
Fragments arranged by size:
2852 1509 685 522 501 477 419 395
175 87 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7649
PvuII CAG'CTG
Cuts at: 585 606 1567 1977 2637 2774 2999 3126 5074
Size: 21 961 410 660 137 225 127 1948
Cuts at: 5074 5434 585
Size: 360 2800
Fragments arranged by size:
2800 1948 961 660 410 360 225 137
127 21
RsrII CG'GwC_CG
Cuts at: 5844 5844
Size: 7649
SacI G_AGCT'C
Cuts at: 524 3560 4631 4663 524
Size: 3036 1071 32 3510
Fragments arranged by size:
3510 3036 1071 32
SacII CC_GC'GG
Cuts at: 506 506
Size: 7649
SalI G'TCGA_C
Cuts at: 4686 4686
Size: 7649
SapI GCTCTTCn'nnn_
Cuts at: 512 2503 3565 5672 5882 512
Size: 1991 1062 2107 210 2279
Fragments arranged by size:
2279 2107 1991 1062 210
SbfI CC_TGCA'GG
Cuts at: 2621 4696 2621
Size: 2075 5574
ScaI AGT'ACT
Cuts at: 455 7166 455
Size: 6711 938
SexAI A'CCwGG_T
Cuts at: 2702 2702
Size: 7649
SfcI C'TryA_G
Cuts at: 33 273 580 607 1108 2617 2709 3139 3314
Size: 240 307 27 501 1509 92 430 175
Cuts at: 3314 3733 3820 4215 4692 5377 6370 6561 33
Size: 419 87 395 477 685 993 191 1121
Fragments arranged by size:
1509 1121 993 685 501 477 430 419
395 307 240 191 175 92 87 27
SfoI GGC'GCC
Cuts at: 3018 5081 5329 3018
Size: 2063 248 5338
Fragments arranged by size:
5338 2063 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7649
SmaI CCC'GGG
Cuts at: 3177 4671 3177
Size: 1494 6155
SmlI C'TyrA_G
Cuts at: 366 685 694 2248 2470 4804 5719 6211 6473
Size: 319 9 1554 222 2334 915 492 262
Cuts at: 6473 366
Size: 1542
Fragments arranged by size:
2334 1554 1542 915 492 319 262 222
9
SnaBI TAC'GTA
Cuts at: 4641 4641
Size: 7649
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7649
SphI G_CATG'C
Cuts at: 4200 4702 5202 4200
Size: 502 500 6647
Fragments arranged by size:
6647 502 500
SspI AAT'ATT
Cuts at: 770 770
Size: 7649
StuI AGG'CCT
Cuts at: 3364 4183 3364
Size: 819 6830
StyI C'CwwG_G
Cuts at: 125 173 1163 1391 1502 1650 1754 3677 4783
Size: 48 990 228 111 148 104 1923 1106
Cuts at: 4783 5137 125
Size: 354 2637
Fragments arranged by size:
2637 1923 1106 990 354 228 148 111
104 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 2935 4199 4391 5232 5365 5824 6033 664
Size: 2271 1264 192 841 133 459 209 2280
Fragments arranged by size:
2280 2271 1264 841 459 209 192 133
TatI w'GTAC_w
Cuts at: 453 3686 7164 453
Size: 3233 3478 938
Fragments arranged by size:
3478 3233 938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1593 1625 2371 2403 4588 4620 7166 7198 1593
Size: 32 746 32 2185 32 2546 32 2044
Fragments arranged by size:
2546 2185 2044 746 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4285 5446 4285
Size: 1161 6488
XbaI T'CTAG_A
Cuts at: 4680 4680
Size: 7649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 238 2979 4032 5144 238
Size: 2741 1053 1112 2743
Fragments arranged by size:
2743 2741 1112 1053
XmaI C'CCGG_G
Cuts at: 3175 4669 3175
Size: 1494 6155
XmnI GAAnn'nnTTC
Cuts at: 1172 1172
Size: 7649
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SanDI SfiI
SgrAI SrfI SwaI XhoI ZraI