Restriction map for
pF1KSDA0976
(Circular) (Six-base) MAPSORT of: pF1KSDA0976.seq Check: 4673 from: 1 to: 4231
pF1KSDA0976 4231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 766 1267 766
Size: 501 3730
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4231
AclI AA'CG_TT
Cuts at: 4071 4071
Size: 4231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2486 3235 3503 2054
Size: 432 749 268 2782
Fragments arranged by size:
2782 749 432 268
AflIII A'CryG_T
Cuts at: 383 1581 2687 3664 383
Size: 1198 1106 977 950
Fragments arranged by size:
1198 1106 977 950
AgeI A'CCGG_T
Cuts at: 2619 2619
Size: 4231
AlwNI CAG_nnn'CTG
Cuts at: 1534 3103 1534
Size: 1569 2662
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4231
ApoI r'AATT_y
Cuts at: 1233 3584 3840 1233
Size: 2351 256 1624
Fragments arranged by size:
2351 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4231
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4231
BamHI G'GATC_C
Cuts at: 690 1254 690
Size: 564 3667
BanI G'GyrC_C
Cuts at: 1245 1659 1907 1942 1245
Size: 414 248 35 3534
Fragments arranged by size:
3534 414 248 35
BanII G_rGCy'C
Cuts at: 1243 2273 1243
Size: 1030 3201
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3983
BbsI GAAGACnn'nnnn_
Cuts at: 465 465
Size: 4231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 995 1029 995
Size: 34 4197
BciVI GTATCCnnnnn_n'
Cuts at: 512 861 1198 2127 2890 4231 512
Size: 349 337 929 763 1341 512
Fragments arranged by size:
1341 929 763 512 349 337
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4231
BglII A'GATC_T
Cuts at: 1744 3783 1744
Size: 2039 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4231
Bme1580I G_kGCm'C
Cuts at: 1854 1947 3005 1854
Size: 93 1058 3080
Fragments arranged by size:
3080 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1187 1441 1522 1857 4003 1187
Size: 254 81 335 2146 1415
Fragments arranged by size:
2146 1415 335 254 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 631 499
Size: 132 4099
Bpu10I CC'TnA_GC
Cuts at: 416 434 416
Size: 18 4213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2778 3076 1405
Size: 879 494 298 2560
Fragments arranged by size:
2560 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 338 3935 338
Size: 3597 634
BsaAI yAC'GTr
Cuts at: 1221 1582 1221
Size: 361 3870
BsaBI GATnn'nnATC
Cuts at: 6 1743 3782 6
Size: 1737 2039 455
Fragments arranged by size:
2039 1737 455
BsaHI Gr'CG_yC
Cuts at: 591 1660 1908 2612 4108 591
Size: 1069 248 704 1496 714
Fragments arranged by size:
1496 1069 714 704 248
BsaWI w'CCGG_w
Cuts at: 327 1418 1939 2619 2893 3040 3451 327
Size: 1091 521 680 274 147 411 1107
Fragments arranged by size:
1107 1091 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1654 2412 2991 3487 1654
Size: 758 579 496 2398
Fragments arranged by size:
2398 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3027 104
Size: 1667 46 1210 1308
Fragments arranged by size:
1667 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1082 1243 2021 2211 3005 1082
Size: 161 778 190 794 2308
Fragments arranged by size:
2308 794 778 190 161
BsmBI CGTCTCn'nnnn_
Cuts at: 349 349
Size: 4231
Bsp1286I G_dGCh'C
Cuts at: 1082 1243 1854 1947 2021 2211 2273 3005 1082
Size: 161 611 93 74 190 62 732 2308
Fragments arranged by size:
2308 732 611 190 161 93 74 62
BspEI T'CCGG_A
Cuts at: 327 3451 327
Size: 3124 1107
BspHI T'CATG_A
Cuts at: 3407 3407
Size: 4231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1279 1493 1795 2176 1279
Size: 214 302 381 3334
Fragments arranged by size:
3334 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4061 4226 50
Size: 2471 54 1486 165 55
Fragments arranged by size:
2471 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 2141 374
Size: 1767 2464
BsrFI r'CCGG_y
Cuts at: 2227 2408 2619 2227
Size: 181 211 3839
Fragments arranged by size:
3839 211 181
BssSI C'ACGA_G
Cuts at: 2500 2860 2500
Size: 360 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4231
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4231
BstYI r'GATC_y
Cuts at: 1 690 1254 1721 1744 2079 2325 3339 3425
Size: 689 564 467 23 335 246 1014 86
Cuts at: 3425 3437 3783 1
Size: 12 346 449
Fragments arranged by size:
1014 689 564 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 767 767
Size: 4231
Bsu36I CC'TnA_GG
Cuts at: 910 910
Size: 4231
BtgI C'CryG_G
Cuts at: 287 287
Size: 4231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3887
Fragments arranged by size:
3887 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1472 1172
Size: 300 3931
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4231
DrdI GACnn_nn'nnGTC
Cuts at: 1139 1935 2795 1139
Size: 796 860 2575
Fragments arranged by size:
2575 860 796
EaeI y'GGCC_r
Cuts at: 1814 1988 2379 2406 3753 1814
Size: 174 391 27 1347 2292
Fragments arranged by size:
2292 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4231
EarI CTCTTCn'nnn_
Cuts at: 743 2252 2462 743
Size: 1509 210 2512
Fragments arranged by size:
2512 1509 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2749 2895 4041 2437
Size: 312 146 1146 2627
Fragments arranged by size:
2627 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 631 2054 2486 3235 3503 499
Size: 132 1423 432 749 268 1227
Fragments arranged by size:
1423 1227 749 432 268 132
EcoO109I rG'GnC_Cy
Cuts at: 1368 1368
Size: 4231
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4231
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4231
HaeII r_GCGC'y
Cuts at: 1663 1911 2935 3914 1663
Size: 248 1024 979 1980
Fragments arranged by size:
1980 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 288 320 682 714 2071 2101 2103 2133 288
Size: 32 362 32 1357 30 2 30 2386
Fragments arranged by size:
2386 1357 362 32 32 30 30 2
HincII GTy'rAC
Cuts at: 522 1268 522
Size: 746 3485
HpaI GTT'AAC
Cuts at: 522 522
Size: 4231
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3983
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4231
MluI A'CGCG_T
Cuts at: 3664 3664
Size: 4231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2902 3086 2902
Size: 184 4047
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4231
MslI CAynn'nnrTG
Cuts at: 292 1000 1071 2218 2345 292
Size: 708 71 1147 127 2178
Fragments arranged by size:
2178 1147 708 127 71
MspA1I CmG'CkG
Cuts at: 864 1340 1654 2014 3029 3274 864
Size: 476 314 360 1015 245 1821
Fragments arranged by size:
1821 1015 476 360 314 245
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4231
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3983
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4231
NruI TCG'CGA
Cuts at: 2592 2592
Size: 4231
NspI r_CATG'y
Cuts at: 387 1070 1282 1782 2691 387
Size: 683 212 500 909 1927
Fragments arranged by size:
1927 909 683 500 212
PciI A'CATG_T
Cuts at: 383 2687 383
Size: 2304 1927
PflMI CCAn_nnn'nTGG
Cuts at: 476 937 1102 476
Size: 461 165 3605
Fragments arranged by size:
3605 461 165
PfoI T'CCnGG_A
Cuts at: 608 608
Size: 4231
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3402 3370
Size: 32 4199
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3546
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4231
PvuII CAG'CTG
Cuts at: 864 1654 2014 864
Size: 790 360 3081
Fragments arranged by size:
3081 790 360
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4231
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4231
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4231
SapI GCTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4021
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4231
ScaI AGT'ACT
Cuts at: 754 3748 754
Size: 2994 1237
SfcI C'TryA_G
Cuts at: 33 1088 1272 1957 2952 3143 33
Size: 1055 184 685 995 191 1121
Fragments arranged by size:
1121 1055 995 685 191 184
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4231
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4231
SmlI C'TyrA_G
Cuts at: 1384 2299 2793 3055 1384
Size: 915 494 262 2560
Fragments arranged by size:
2560 915 494 262
SnaBI TAC'GTA
Cuts at: 1221 1221
Size: 4231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4231
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3731
SspI AAT'ATT
Cuts at: 947 947
Size: 4231
StyI C'CwwG_G
Cuts at: 1135 1363 1717 1135
Size: 228 354 3649
Fragments arranged by size:
3649 354 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2615 1812
Size: 133 459 211 3428
Fragments arranged by size:
3428 459 211 133
TatI w'GTAC_w
Cuts at: 752 3746 752
Size: 2994 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1077 1109 3748 3780 1077
Size: 32 2639 32 1528
Fragments arranged by size:
2639 1528 32 32
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4231
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4231
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4231
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI XmnI ZraI