Restriction map for
pF1KSDA0988
(Circular) (Six-base) MAPSORT of: pF1KSDA0988.seq Check: 7104 from: 1 to: 6715
pF1KSDA0988 6715 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 1187 3551 1187
Size: 2364 4351
AccI GT'mk_AC
Cuts at: 3112 3751 3112
Size: 639 6076
Acc65I G'GTAC_C
Cuts at: 1076 3729 1076
Size: 2653 4062
AclI AA'CG_TT
Cuts at: 6555 6555
Size: 6715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1252 1329 1480 1594 2110 3174 3277 4538
Size: 180 77 151 114 516 1064 103 1261
Cuts at: 4538 4970 5719 5987 1072
Size: 432 749 268 1800
Fragments arranged by size:
1800 1261 1064 749 516 432 268 180
151 114 103 77
AflIII A'CryG_T
Cuts at: 772 1532 4065 5171 6148 772
Size: 760 2533 1106 977 1339
Fragments arranged by size:
2533 1339 1106 977 760
AgeI A'CCGG_T
Cuts at: 5103 5103
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 1958 1958
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1249 1249
Size: 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3426 3426
Size: 6715
AlwNI CAG_nnn'CTG
Cuts at: 748 894 999 1035 1221 1572 1657 1847 2088
Size: 146 105 36 186 351 85 190 241
Cuts at: 2088 2406 3180 4018 5587 748
Size: 318 774 838 1569 1876
Fragments arranged by size:
1876 1569 838 774 351 318 241 190
186 146 105 85 36
ApaLI G'TGCA_C
Cuts at: 262 1601 3005 5485 262
Size: 1339 1404 2480 1492
Fragments arranged by size:
2480 1492 1404 1339
ApoI r'AATT_y
Cuts at: 436 3366 3717 6068 6324 436
Size: 2930 351 2351 256 827
Fragments arranged by size:
2930 2351 827 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6715
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6715
AvaI C'yCGr_G
Cuts at: 160 233 734 1596 1909 1935 2425 2542 3733
Size: 73 501 862 313 26 490 117 1191
Cuts at: 3733 160
Size: 3142
Fragments arranged by size:
3142 1191 862 501 490 313 117 73
26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1068 1101 1068
Size: 33 6682
BamHI G'GATC_C
Cuts at: 3738 3738
Size: 6715
BanI G'GyrC_C
Cuts at: 959 1076 1489 1504 1905 2464 2728 3681 3694
Size: 117 413 15 401 559 264 953 13
Cuts at: 3694 3729 4143 4391 4426 959
Size: 35 414 248 35 3248
Fragments arranged by size:
3248 953 559 414 413 401 264 248
117 35 35 15 13
BanII G_rGCy'C
Cuts at: 1308 2631 3727 4757 1308
Size: 1323 1096 1030 3266
Fragments arranged by size:
3266 1323 1096 1030
BbeI G_GCGC'C
Cuts at: 2468 4147 4395 2468
Size: 1679 248 4788
Fragments arranged by size:
4788 1679 248
BbsI GAAGACnn'nnnn_
Cuts at: 932 1923 3265 3508 932
Size: 991 1342 243 4139
Fragments arranged by size:
4139 1342 991 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1866 1900 2297 2331 1866
Size: 34 397 34 6250
Fragments arranged by size:
6250 397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4611 5374 6715 4611
Size: 763 1341 4611
Fragments arranged by size:
4611 1341 763
BclI T'GATC_A
Cuts at: 2393 4233 2393
Size: 1840 4875
BglI GCCn_nnn'nGGC
Cuts at: 968 1903 2851 968
Size: 935 948 4832
Fragments arranged by size:
4832 948 935
BglII A'GATC_T
Cuts at: 1965 3209 4228 6267 1965
Size: 1244 1019 2039 2413
Fragments arranged by size:
2413 2039 1244 1019
BlpI GC'TnA_GC
Cuts at: 2411 3825 2411
Size: 1414 5301
Bme1580I G_kGCm'C
Cuts at: 266 1507 1605 1693 2731 2945 3009 3686 4338
Size: 1241 98 88 1038 214 64 677 652
Cuts at: 4338 4431 5489 266
Size: 93 1058 1492
Fragments arranged by size:
1492 1241 1058 1038 677 652 214 98
93 88 64
BmgBI CAC'GTC
Cuts at: 775 2724 3069 3438 775
Size: 1949 345 369 4052
Fragments arranged by size:
4052 1949 369 345
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1857 3925 4006 4341 6487 1045
Size: 812 2068 81 335 2146 1273
Fragments arranged by size:
2146 2068 1273 812 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 5080 2164
Size: 2916 3799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 392 837 2482 3021 316
Size: 76 445 1645 539 4010
Fragments arranged by size:
4010 1645 539 445 76
Bpu10I CC'TnA_GC
Cuts at: 133 2798 133
Size: 2665 4050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2262 3889 4768 5262 5560 2262
Size: 1627 879 494 298 3417
Fragments arranged by size:
3417 1627 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 223 6419 223
Size: 6196 519
BsaAI yAC'GTr
Cuts at: 1533 2940 3273 3705 4066 1533
Size: 1407 333 432 361 4182
Fragments arranged by size:
4182 1407 432 361 333
BsaBI GATnn'nnATC
Cuts at: 6 4227 6266 6
Size: 4221 2039 455
Fragments arranged by size:
4221 2039 455
BsaHI Gr'CG_yC
Cuts at: 1184 1520 2465 3548 4144 4392 5096 6592 1184
Size: 336 945 1083 596 248 704 1496 1307
Fragments arranged by size:
1496 1307 1083 945 704 596 336 248
BsaWI w'CCGG_w
Cuts at: 3497 3902 4423 5103 5377 5524 5935 3497
Size: 405 521 680 274 147 411 4277
Fragments arranged by size:
4277 680 521 411 405 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2642 2672 2642
Size: 30 6685
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 181 637 1108 2401 2443 2966 3325 3456
Size: 3 456 471 1293 42 523 359 131
Cuts at: 3456 3463 178
Size: 7 3430
Fragments arranged by size:
3430 1293 523 471 456 359 131 42
7 3
BseYI C'CCAG_C
Cuts at: 243 653 1011 1401 1898 2682 2846 3035 3075
Size: 410 358 390 497 784 164 189 40
Cuts at: 3075 3674 3686 4138 4896 5475 5971 243
Size: 599 12 452 758 579 496 987
Fragments arranged by size:
987 784 758 599 579 497 496 452
410 390 358 189 164 40 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1870 2101 2896 3442 1048
Size: 822 231 795 546 4321
Fragments arranged by size:
4321 822 795 546 231
BsiEI CG_ry'CG
Cuts at: 104 149 1250 4255 4301 5511 104
Size: 45 1101 3005 46 1210 1308
Fragments arranged by size:
3005 1308 1210 1101 46 45
BsiHKAI G_wGCw'C
Cuts at: 266 1605 2883 3009 3278 3590 3727 4505 4695
Size: 1339 1278 126 269 312 137 778 190
Cuts at: 4695 5489 266
Size: 794 1492
Fragments arranged by size:
1492 1339 1278 794 778 312 269 190
137 126
BsmI GAATG_Cn'
Cuts at: 3158 3158
Size: 6715
BsmBI CGTCTCn'nnnn_
Cuts at: 1433 2567 1433
Size: 1134 5581
Bsp1286I G_dGCh'C
Cuts at: 266 1308 1507 1605 1693 2631 2731 2883 2945
Size: 1042 199 98 88 938 100 152 62
Cuts at: 2945 3009 3278 3590 3686 3727 4338 4431 4505
Size: 64 269 312 96 41 611 93 74
Cuts at: 4505 4695 4757 5489 266
Size: 190 62 732 1492
Fragments arranged by size:
1492 1042 938 732 611 312 269 199
190 152 100 98 96 93 88 74
64 62 62 41
BspEI T'CCGG_A
Cuts at: 3497 5935 3497
Size: 2438 4277
BspHI T'CATG_A
Cuts at: 495 2150 5891 495
Size: 1655 3741 1319
Fragments arranged by size:
3741 1655 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 613 2493 3763 3977 4279 4660 613
Size: 1880 1270 214 302 381 2668
Fragments arranged by size:
2668 1880 1270 381 302 214
BsrBI CCG'CTC
Cuts at: 50 300 5005 5059 6545 6710 50
Size: 250 4705 54 1486 165 55
Fragments arranged by size:
4705 1486 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2242 2588 3529 4625 2242
Size: 346 941 1096 4332
Fragments arranged by size:
4332 1096 941 346
BsrFI r'CCGG_y
Cuts at: 144 1786 2492 4711 4892 5103 144
Size: 1642 706 2219 181 211 1756
Fragments arranged by size:
2219 1756 1642 706 211 181
BsrGI T'GTAC_A
Cuts at: 420 2021 420
Size: 1601 5114
BssSI C'ACGA_G
Cuts at: 1435 4984 5344 1435
Size: 3549 360 2806
Fragments arranged by size:
3549 2806 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1657 2008 2079 2834 4090 1657
Size: 351 71 755 1256 4282
Fragments arranged by size:
4282 1256 755 351 71
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1827 1827
Size: 6715
BstXI CCAn_nnnn'nTGG
Cuts at: 1308 1852 2611 2763 3043 1308
Size: 544 759 152 280 4980
Fragments arranged by size:
4980 759 544 280 152
BstYI r'GATC_y
Cuts at: 1 1750 1965 2370 2766 3209 3738 4205 4228
Size: 1749 215 405 396 443 529 467 23
Cuts at: 4228 4563 4809 5823 5909 5921 6267 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1749 1014 529 467 449 443 405 396
346 335 246 215 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 955 1565 955
Size: 610 6105
BtgI C'CryG_G
Cuts at: 125 149 872 2705 3282 3305 3506 125
Size: 24 723 1833 577 23 201 3334
Fragments arranged by size:
3334 1833 723 577 201 24 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3384 4663 4844 5007 3384
Size: 1279 181 163 5092
Fragments arranged by size:
5092 1279 181 163
BtsI GCAGTG_nn'
Cuts at: 2084 2168 2343 3410 3596 3626 3956 2084
Size: 84 175 1067 186 30 330 4843
Fragments arranged by size:
4843 1067 330 186 175 84 30
DraI TTT'AAA
Cuts at: 435 909 3712 435
Size: 474 2803 3438
Fragments arranged by size:
3438 2803 474
DraIII CAC_nnn'GTG
Cuts at: 3602 3602
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 4419 5279 4419
Size: 860 5855
EaeI y'GGCC_r
Cuts at: 146 3509 4298 4472 4863 4890 6237 146
Size: 3363 789 174 391 27 1347 624
Fragments arranged by size:
3363 1347 789 624 391 174 27
EagI C'GGCC_G
Cuts at: 146 4298 146
Size: 4152 2563
EarI CTCTTCn'nnn_
Cuts at: 1641 4736 4946 1641
Size: 3095 210 3410
Fragments arranged by size:
3410 3095 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 1327 4921 5233 5379 6525 187
Size: 1140 3594 312 146 1146 377
Fragments arranged by size:
3594 1146 1140 377 312 146
EcoICRI GAG'CTC
Cuts at: 3725 3725
Size: 6715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 392 837 1072 1252 1329 1480 1594 2110
Size: 76 445 235 180 77 151 114 516
Cuts at: 2110 2482 3021 3174 3277 4538 4970 5719 5987
Size: 372 539 153 103 1261 432 749 268
Cuts at: 5987 316
Size: 1044
Fragments arranged by size:
1261 1044 749 539 516 445 432 372
268 235 180 153 151 114 103 77
76
EcoNI CCTnn'n_nnAGG
Cuts at: 256 1123 256
Size: 867 5848
EcoO109I rG'GnC_Cy
Cuts at: 891 2085 3177 3197 3450 3671 3852 891
Size: 1194 1092 20 253 221 181 3754
Fragments arranged by size:
3754 1194 1092 253 221 181 20
EcoRI G'AATT_C
Cuts at: 3366 3717 3366
Size: 351 6364
EcoRV GAT'ATC
Cuts at: 3378 3378
Size: 6715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4732 4732
Size: 6715
FspI TGC'GCA
Cuts at: 2001 4494 2001
Size: 2493 4222
HaeII r_GCGC'y
Cuts at: 205 241 1393 1468 1885 1922 2468 4147 4395
Size: 36 1152 75 417 37 546 1679 248
Cuts at: 4395 5419 6398 205
Size: 1024 979 522
Fragments arranged by size:
1679 1152 1024 979 546 522 417 248
75 37 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 1944 1950 1976 1982 2974 3006 3226
Size: 32 1524 6 26 6 992 32 220
Cuts at: 3226 3258 4555 4585 4587 4617 388
Size: 32 1297 30 2 30 2486
Fragments arranged by size:
2486 1524 1297 992 220 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 3113 3752 3113
Size: 639 6076
HindIII A'AGCT_T
Cuts at: 560 1021 560
Size: 461 6254
KasI G'GCGC_C
Cuts at: 2464 4143 4391 2464
Size: 1679 248 4788
Fragments arranged by size:
4788 1679 248
KpnI G_GTAC'C
Cuts at: 1080 3733 1080
Size: 2653 4062
MluI A'CGCG_T
Cuts at: 6148 6148
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 362 1778 5386 5570 326
Size: 36 1416 3608 184 1471
Fragments arranged by size:
3608 1471 1416 184 36
MscI TGG'CCA
Cuts at: 4474 4474
Size: 6715
MslI CAynn'nnrTG
Cuts at: 871 1249 1850 2819 3403 4702 4829 871
Size: 378 601 969 584 1299 127 2757
Fragments arranged by size:
2757 1299 969 601 584 378 127
MspA1I CmG'CkG
Cuts at: 151 745 1218 1726 3194 3252 3462 3492 3678
Size: 594 473 508 1468 58 210 30 186
Cuts at: 3678 3824 4138 4498 5513 5758 151
Size: 146 314 360 1015 245 1108
Fragments arranged by size:
1468 1108 1015 594 508 473 360 314
245 210 186 146 58 30
NaeI GCC'GGC
Cuts at: 1788 2494 4894 1788
Size: 706 2400 3609
Fragments arranged by size:
3609 2400 706
NarI GG'CG_CC
Cuts at: 2465 4144 4392 2465
Size: 1679 248 4788
Fragments arranged by size:
4788 1679 248
NcoI C'CATG_G
Cuts at: 125 872 125
Size: 747 5968
NgoMIV G'CCGG_C
Cuts at: 1786 2492 4892 1786
Size: 706 2400 3609
Fragments arranged by size:
3609 2400 706
NruI TCG'CGA
Cuts at: 5076 5076
Size: 6715
NspI r_CATG'y
Cuts at: 582 3766 4266 5175 582
Size: 3184 500 909 2122
Fragments arranged by size:
3184 2122 909 500
PasI CC'CwG_GG
Cuts at: 3093 3180 3093
Size: 87 6628
PciI A'CATG_T
Cuts at: 5171 5171
Size: 6715
PfoI T'CCnGG_A
Cuts at: 809 1352 1667 2566 809
Size: 543 315 899 4958
Fragments arranged by size:
4958 899 543 315
PmeI GTTT'AAAC
Cuts at: 3712 3712
Size: 6715
PmlI CAC'GTG
Cuts at: 2940 3273 2940
Size: 333 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5854 5886 5854
Size: 32 6683
PpuMI rG'GwC_Cy
Cuts at: 3197 3197
Size: 6715
PsiI TTA'TAA
Cuts at: 1771 1771
Size: 6715
PstI C_TGCA'G
Cuts at: 890 976 1115 1266 1498 1645 3209 3461 3760
Size: 86 139 151 232 147 1564 252 299
Cuts at: 3760 4445 890
Size: 685 3160
Fragments arranged by size:
3160 1564 685 299 252 232 151 147
139 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6715
PvuII CAG'CTG
Cuts at: 745 1218 1726 3462 3678 4138 4498 745
Size: 473 508 1736 216 460 360 2962
Fragments arranged by size:
2962 1736 508 473 460 360 216
RsrII CG'GwC_CG
Cuts at: 4908 4908
Size: 6715
SacI G_AGCT'C
Cuts at: 3727 3727
Size: 6715
SacII CC_GC'GG
Cuts at: 152 152
Size: 6715
SalI G'TCGA_C
Cuts at: 3111 3750 3111
Size: 639 6076
SanDI GG'GwC_CC
Cuts at: 3197 3197
Size: 6715
SapI GCTCTTCn'nnn_
Cuts at: 1641 4736 4946 1641
Size: 3095 210 3410
Fragments arranged by size:
3410 3095 210
SbfI CC_TGCA'GG
Cuts at: 3760 3760
Size: 6715
ScaI AGT'ACT
Cuts at: 6232 6232
Size: 6715
SexAI A'CCwGG_T
Cuts at: 1823 1823
Size: 6715
SfcI C'TryA_G
Cuts at: 33 886 972 1111 1262 1494 1641 3205 3457
Size: 853 86 139 151 232 147 1564 252
Cuts at: 3457 3540 3756 4441 5436 5627 33
Size: 83 216 685 995 191 1121
Fragments arranged by size:
1564 1121 995 853 685 252 232 216
191 151 147 139 86 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 2851 2851
Size: 6715
SfoI GGC'GCC
Cuts at: 2466 4145 4393 2466
Size: 1679 248 4788
Fragments arranged by size:
4788 1679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6715
SmaI CCC'GGG
Cuts at: 235 3735 235
Size: 3500 3215
SmlI C'TyrA_G
Cuts at: 1596 2277 3868 4783 5277 5539 1596
Size: 681 1591 915 494 262 2772
Fragments arranged by size:
2772 1591 915 681 494 262
SnaBI TAC'GTA
Cuts at: 3705 3705
Size: 6715
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6715
SphI G_CATG'C
Cuts at: 3766 4266 3766
Size: 500 6215
SspI AAT'ATT
Cuts at: 905 2344 905
Size: 1439 5276
StuI AGG'CCT
Cuts at: 1415 3019 1415
Size: 1604 5111
StyI C'CwwG_G
Cuts at: 125 455 548 872 1268 2459 3042 3847 4201
Size: 330 93 324 396 1191 583 805 354
Cuts at: 4201 125
Size: 2639
Fragments arranged by size:
2639 1191 805 583 396 354 330 324
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 1174 1643 3049 3145 3295 4296 4429 4888
Size: 507 469 1406 96 150 1001 133 459
Cuts at: 4888 5099 667
Size: 211 2283
Fragments arranged by size:
2283 1406 1001 507 469 459 211 150
133 96
TatI w'GTAC_w
Cuts at: 420 1799 2021 3146 6230 420
Size: 1379 222 1125 3084 905
Fragments arranged by size:
3084 1379 1125 905 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6232 6264 6232
Size: 32 6683
Tth111I GACn'n_nGTC
Cuts at: 1183 1246 3108 4510 1183
Size: 63 1862 1402 3388
Fragments arranged by size:
3388 1862 1402 63
XbaI T'CTAG_A
Cuts at: 3744 3744
Size: 6715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1761 2982 3675 4208 1761
Size: 1221 693 533 4268
Fragments arranged by size:
4268 1221 693 533
XhoI C'TCGA_G
Cuts at: 1596 1596
Size: 6715
XmaI C'CCGG_G
Cuts at: 233 3733 233
Size: 3500 3215
ZraI GAC'GTC
Cuts at: 1185 3549 1185
Size: 2364 4351
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI ApaI AscI AvrII BbvCI
BfrBI BmtI BsiWI BssHII BstZ17I ClaI CspCI FseI
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PflMI PshAI PspOMI PspXI PsrI SgrAI SrfI SwaI
XmnI