Restriction map for
pF1KSDA0989
(Circular) (Six-base) MAPSORT of: pF1KSDA0989.seq Check: 4726 from: 1 to: 5479
pF1KSDA0989 5479 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1005 1699 2353 1005
Size: 694 654 4131
Fragments arranged by size:
4131 694 654
AccI GT'mk_AC
Cuts at: 2515 2515
Size: 5479
Acc65I G'GTAC_C
Cuts at: 815 2493 815
Size: 1678 3801
AclI AA'CG_TT
Cuts at: 5319 5319
Size: 5479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 872 2047 3302 3734 4483 4751 598
Size: 274 1175 1255 432 749 268 1326
Fragments arranged by size:
1326 1255 1175 749 432 274 268
AfeI AGC'GCT
Cuts at: 2071 2071
Size: 5479
AflIII A'CryG_T
Cuts at: 2829 3935 4912 2829
Size: 1106 977 3396
Fragments arranged by size:
3396 1106 977
AgeI A'CCGG_T
Cuts at: 3867 3867
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 240 321 918 1509 1803 2034 2782 4351 240
Size: 81 597 591 294 231 748 1569 1368
Fragments arranged by size:
1569 1368 748 597 591 294 231 81
ApaI G_GGCC'C
Cuts at: 534 648 706 721 736 534
Size: 114 58 15 15 5277
Fragments arranged by size:
5277 114 58 15 15
ApaLI G'TGCA_C
Cuts at: 1691 4249 1691
Size: 2558 2921
ApoI r'AATT_y
Cuts at: 2481 4832 5088 2481
Size: 2351 256 2872
Fragments arranged by size:
2872 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5479
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5479
AvaI C'yCGr_G
Cuts at: 147 534 641 743 1041 1535 1603 2497 147
Size: 387 107 102 298 494 68 894 3129
Fragments arranged by size:
3129 894 494 387 298 107 102 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 745 778 745
Size: 33 5446
BamHI G'GATC_C
Cuts at: 860 2502 860
Size: 1642 3837
BanI G'GyrC_C
Cuts at: 637 815 876 1462 1711 2127 2493 2907 3155
Size: 178 61 586 249 416 366 414 248
Cuts at: 3155 3190 637
Size: 35 2926
Fragments arranged by size:
2926 586 416 414 366 249 248 178
61 35
BanII G_rGCy'C
Cuts at: 277 347 534 648 664 706 721 736 985
Size: 70 187 114 16 42 15 15 249
Cuts at: 985 1138 1754 2107 2180 2491 3521 277
Size: 153 616 353 73 311 1030 2235
Fragments arranged by size:
2235 1030 616 353 311 249 187 153
114 73 70 42 16 15 15
BbeI G_GCGC'C
Cuts at: 641 1466 2911 3159 641
Size: 825 1445 248 2961
Fragments arranged by size:
2961 1445 825 248
BbsI GAAGACnn'nnnn_
Cuts at: 146 1252 146
Size: 1106 4373
BbvCI CC'TCA_GC
Cuts at: 574 728 1032 1078 574
Size: 154 304 46 4975
Fragments arranged by size:
4975 304 154 46
BciVI GTATCCnnnnn_n'
Cuts at: 3375 4138 5479 3375
Size: 763 1341 3375
Fragments arranged by size:
3375 1341 763
BclI T'GATC_A
Cuts at: 2051 2997 2051
Size: 946 4533
BglI GCCn_nnn'nGGC
Cuts at: 487 1057 487
Size: 570 4909
BglII A'GATC_T
Cuts at: 2992 5031 2992
Size: 2039 3440
BlpI GC'TnA_GC
Cuts at: 1753 1901 2589 1753
Size: 148 688 4643
Fragments arranged by size:
4643 688 148
Bme1580I G_kGCm'C
Cuts at: 534 587 648 706 721 736 825 1023 1053
Size: 53 61 58 15 15 89 198 30
Cuts at: 1053 1695 1716 3102 3195 4253 534
Size: 642 21 1386 93 1058 1760
Fragments arranged by size:
1760 1386 1058 642 198 93 89 61
58 53 30 21 15 15
BmrI ACTGGGnnnn_n'
Cuts at: 269 2689 2770 3105 5251 269
Size: 2420 81 335 2146 497
Fragments arranged by size:
2420 2146 497 335 81
BmtI G_CTAG'C
Cuts at: 223 223
Size: 5479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3844 3844
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 646 1150 1261 1456 1519 1663 1687 1730
Size: 336 504 111 195 63 144 24 43
Cuts at: 1730 1831 2056 2058 310
Size: 101 225 2 3731
Fragments arranged by size:
3731 504 336 225 195 144 111 101
63 43 24 2
Bpu10I CC'TnA_GC
Cuts at: 574 728 979 1032 1078 574
Size: 154 251 53 46 4975
Fragments arranged by size:
4975 251 154 53 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2653 3532 4026 4324 1663
Size: 990 879 494 298 2818
Fragments arranged by size:
2818 990 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 781 1748 2075 5183 781
Size: 967 327 3108 1077
Fragments arranged by size:
3108 1077 967 327
BsaAI yAC'GTr
Cuts at: 588 2469 2830 588
Size: 1881 361 3237
Fragments arranged by size:
3237 1881 361
BsaBI GATnn'nnATC
Cuts at: 6 1320 2461 2991 5030 6
Size: 1314 1141 530 2039 455
Fragments arranged by size:
2039 1314 1141 530 455
BsaHI Gr'CG_yC
Cuts at: 638 1463 2207 2908 3156 3860 5356 638
Size: 825 744 701 248 704 1496 761
Fragments arranged by size:
1496 825 761 744 704 701 248
BsaWI w'CCGG_w
Cuts at: 2355 2666 3187 3867 4141 4288 4699 2355
Size: 311 521 680 274 147 411 3135
Fragments arranged by size:
3135 680 521 411 311 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 1825 1855 2323 2353 646
Size: 30 1149 30 468 30 3772
Fragments arranged by size:
3772 1149 468 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 445 463 654 1020 1212 1293 1561 1807
Size: 310 18 191 366 192 81 268 246
Cuts at: 1807 2263 135
Size: 456 3351
Fragments arranged by size:
3351 456 366 310 268 246 192 191
81 18
BseYI C'CCAG_C
Cuts at: 418 482 649 1934 1945 2902 3660 4239 4735
Size: 64 167 1285 11 957 758 579 496
Cuts at: 4735 418
Size: 1162
Fragments arranged by size:
1285 1162 957 758 579 496 167 64
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 2400 145
Size: 2255 3224
BsiEI CG_ry'CG
Cuts at: 104 3019 3065 4275 104
Size: 2915 46 1210 1308
Fragments arranged by size:
2915 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 362 664 938 985 1138 1695 1754 2021 2241
Size: 302 274 47 153 557 59 267 220
Cuts at: 2241 2311 2491 3269 3459 4253 362
Size: 70 180 778 190 794 1588
Fragments arranged by size:
1588 794 778 557 302 274 267 220
190 180 153 70 59 47
BsmI GAATG_Cn'
Cuts at: 1092 1092
Size: 5479
BsmBI CGTCTCn'nnnn_
Cuts at: 199 199
Size: 5479
Bsp1286I G_dGCh'C
Cuts at: 277 347 362 534 587 648 664 706 721
Size: 70 15 172 53 61 16 42 15
Cuts at: 721 736 825 938 985 1023 1053 1138 1695
Size: 15 89 113 47 38 30 85 557
Cuts at: 1695 1716 1754 2021 2107 2180 2241 2311 2491
Size: 21 38 267 86 73 61 70 180
Cuts at: 2491 3102 3195 3269 3459 3521 4253 277
Size: 611 93 74 190 62 732 1503
Fragments arranged by size:
1503 732 611 557 267 190 180 172
113 93 89 86 85 74 73 70
70 62 61 61 53 47 42 38
38 30 21 16 15 15 15
BspEI T'CCGG_A
Cuts at: 4699 4699
Size: 5479
BspHI T'CATG_A
Cuts at: 783 4655 783
Size: 3872 1607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1005 1699 1997 2353 2527 2741 3043 3424
Size: 79 694 298 356 174 214 302 381
Cuts at: 3424 926
Size: 2981
Fragments arranged by size:
2981 694 381 356 302 298 214 174
79
BsrBI CCG'CTC
Cuts at: 50 1113 1587 3769 3823 5309 5474 50
Size: 1063 474 2182 54 1486 165 55
Fragments arranged by size:
2182 1486 1063 474 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1521 3389 1521
Size: 1868 3611
BsrFI r'CCGG_y
Cuts at: 401 1366 3475 3656 3867 401
Size: 965 2109 181 211 2013
Fragments arranged by size:
2109 2013 965 211 181
BssSI C'ACGA_G
Cuts at: 3748 4108 3748
Size: 360 5119
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 2854
Size: 5479
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5479
BstYI r'GATC_y
Cuts at: 1 293 519 860 1302 1799 2030 2502 2969
Size: 292 226 341 442 497 231 472 467
Cuts at: 2969 2992 3327 3573 4587 4673 4685 5031 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 497 472 467 449 442 346 341
335 292 246 231 226 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 241 241
Size: 5479
BtgI C'CryG_G
Cuts at: 525 811 825 969 1874 1889 525
Size: 286 14 144 905 15 4115
Fragments arranged by size:
4115 905 286 144 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3427 3608 3771 3427
Size: 181 163 5135
Fragments arranged by size:
5135 181 163
BtsI GCAGTG_nn'
Cuts at: 794 1053 1450 1734 1740 2054 2720 794
Size: 259 397 284 6 314 666 3553
Fragments arranged by size:
3553 666 397 314 284 259 6
DraI TTT'AAA
Cuts at: 2476 2476
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 3183 4043 3183
Size: 860 4619
EaeI y'GGCC_r
Cuts at: 499 966 1152 1385 1980 3062 3236 3627 3654
Size: 467 186 233 595 1082 174 391 27
Cuts at: 3654 5001 499
Size: 1347 977
Fragments arranged by size:
1347 1082 977 595 467 391 233 186
174 27
EagI C'GGCC_G
Cuts at: 3062 3062
Size: 5479
EarI CTCTTCn'nnn_
Cuts at: 1538 2187 3500 3710 1538
Size: 649 1313 210 3307
Fragments arranged by size:
3307 1313 649 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3685 3997 4143 5289 3685
Size: 312 146 1146 3875
Fragments arranged by size:
3875 1146 312 146
EcoICRI GAG'CTC
Cuts at: 662 983 1136 1752 2489 662
Size: 321 153 616 737 3652
Fragments arranged by size:
3652 737 616 321 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 598 646 872 1150 1261 1456 1519 1663
Size: 288 48 226 278 111 195 63 144
Cuts at: 1663 1687 1730 1831 2047 2056 2058 3302 3734
Size: 24 43 101 216 9 2 1244 432
Cuts at: 3734 4483 4751 310
Size: 749 268 1038
Fragments arranged by size:
1244 1038 749 432 288 278 268 226
216 195 144 111 101 63 48 43
24 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 172 1669 2038 172
Size: 1497 369 3613
Fragments arranged by size:
3613 1497 369
EcoO109I rG'GnC_Cy
Cuts at: 237 494 530 531 561 645 702 703 717
Size: 257 36 1 30 84 57 1 14
Cuts at: 717 718 732 748 1236 1683 2058 2616 237
Size: 1 14 16 488 447 375 558 3100
Fragments arranged by size:
3100 558 488 447 375 257 84 57
36 30 16 14 14 1 1 1
EcoRI G'AATT_C
Cuts at: 2481 2481
Size: 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5479
FspI TGC'GCA
Cuts at: 1520 1555 3258 1520
Size: 35 1703 3741
Fragments arranged by size:
3741 1703 35
HaeII r_GCGC'y
Cuts at: 641 1466 1603 1760 1810 2073 2911 3159 4183
Size: 825 137 157 50 263 838 248 1024
Cuts at: 4183 5162 641
Size: 979 958
Fragments arranged by size:
1024 979 958 838 825 263 248 157
137 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2323 2355 3319 3349 3351 3381 2323
Size: 32 964 30 2 30 4421
Fragments arranged by size:
4421 964 32 30 30 2
HincII GTy'rAC
Cuts at: 2516 2516
Size: 5479
KasI G'GCGC_C
Cuts at: 637 1462 2907 3155 637
Size: 825 1445 248 2961
Fragments arranged by size:
2961 1445 825 248
KpnI G_GTAC'C
Cuts at: 819 2497 819
Size: 1678 3801
MluI A'CGCG_T
Cuts at: 4912 4912
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 526 918 1947 4150 4334 526
Size: 392 1029 2203 184 1671
Fragments arranged by size:
2203 1671 1029 392 184
MscI TGG'CCA
Cuts at: 1387 1982 3238 1387
Size: 595 1256 3628
Fragments arranged by size:
3628 1256 595
MslI CAynn'nnrTG
Cuts at: 3466 3593 3466
Size: 127 5352
MspA1I CmG'CkG
Cuts at: 183 684 827 958 1185 1479 1611 1641 2588
Size: 501 143 131 227 294 132 30 947
Cuts at: 2588 2902 3262 4277 4522 183
Size: 314 360 1015 245 1140
Fragments arranged by size:
1140 1015 947 501 360 314 294 245
227 143 132 131 30
NaeI GCC'GGC
Cuts at: 1368 3658 1368
Size: 2290 3189
NarI GG'CG_CC
Cuts at: 638 1463 2908 3156 638
Size: 825 1445 248 2961
Fragments arranged by size:
2961 1445 825 248
NcoI C'CATG_G
Cuts at: 969 1874 969
Size: 905 4574
NgoMIV G'CCGG_C
Cuts at: 1366 3656 1366
Size: 2290 3189
NheI G'CTAG_C
Cuts at: 219 219
Size: 5479
NruI TCG'CGA
Cuts at: 3840 3840
Size: 5479
NspI r_CATG'y
Cuts at: 508 850 2530 3030 3939 508
Size: 342 1680 500 909 2048
Fragments arranged by size:
2048 1680 909 500 342
PasI CC'CwG_GG
Cuts at: 348 2163 348
Size: 1815 3664
PciI A'CATG_T
Cuts at: 3935 3935
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 2034 2034
Size: 5479
PfoI T'CCnGG_A
Cuts at: 173 2033 2074 2432 173
Size: 1860 41 358 3220
Fragments arranged by size:
3220 1860 358 41
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5479
PmlI CAC'GTG
Cuts at: 588 588
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4618 4650 4618
Size: 32 5447
PpuMI rG'GwC_Cy
Cuts at: 494 748 2058 494
Size: 254 1310 3915
Fragments arranged by size:
3915 1310 254
PspOMI G'GGCC_C
Cuts at: 530 644 702 717 732 530
Size: 114 58 15 15 5277
Fragments arranged by size:
5277 114 58 15 15
PspXI vC'TCGA_Gb
Cuts at: 1535 1535
Size: 5479
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 249 281 382 414 249
Size: 32 101 32 5314
Fragments arranged by size:
5314 101 32 32
PstI C_TGCA'G
Cuts at: 326 869 923 1100 1121 1595 1616 2524 3209
Size: 543 54 177 21 474 21 908 685
Cuts at: 3209 326
Size: 2596
Fragments arranged by size:
2596 908 685 543 474 177 54 21
21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5479
PvuII CAG'CTG
Cuts at: 183 684 958 1611 1641 2902 3262 183
Size: 501 274 653 30 1261 360 2400
Fragments arranged by size:
2400 1261 653 501 360 274 30
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5479
SacI G_AGCT'C
Cuts at: 664 985 1138 1754 2491 664
Size: 321 153 616 737 3652
Fragments arranged by size:
3652 737 616 321 153
SacII CC_GC'GG
Cuts at: 828 828
Size: 5479
SalI G'TCGA_C
Cuts at: 2514 2514
Size: 5479
SanDI GG'GwC_CC
Cuts at: 494 494
Size: 5479
SapI GCTCTTCn'nnn_
Cuts at: 3500 3710 3500
Size: 210 5269
SbfI CC_TGCA'GG
Cuts at: 869 2524 869
Size: 1655 3824
ScaI AGT'ACT
Cuts at: 472 4996 472
Size: 4524 955
SfcI C'TryA_G
Cuts at: 33 322 865 919 1096 1117 1591 1612 2225
Size: 289 543 54 177 21 474 21 613
Cuts at: 2225 2414 2520 3205 4200 4391 33
Size: 189 106 685 995 191 1121
Fragments arranged by size:
1121 995 685 613 543 474 289 191
189 177 106 54 21 21
SfoI GGC'GCC
Cuts at: 639 1464 2909 3157 639
Size: 825 1445 248 2961
Fragments arranged by size:
2961 1445 825 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5479
SmaI CCC'GGG
Cuts at: 536 643 2499 536
Size: 107 1856 3516
Fragments arranged by size:
3516 1856 107
SmlI C'TyrA_G
Cuts at: 1535 1678 2632 3547 4041 4303 1535
Size: 143 954 915 494 262 2711
Fragments arranged by size:
2711 954 915 494 262 143
SnaBI TAC'GTA
Cuts at: 2469 2469
Size: 5479
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5479
SphI G_CATG'C
Cuts at: 850 2530 3030 850
Size: 1680 500 3299
Fragments arranged by size:
3299 1680 500
SrfI GCCC'GGGC
Cuts at: 643 643
Size: 5479
StuI AGG'CCT
Cuts at: 283 1027 1567 283
Size: 744 540 4195
Fragments arranged by size:
4195 744 540
StyI C'CwwG_G
Cuts at: 969 1874 2611 2965 969
Size: 905 737 354 3483
Fragments arranged by size:
3483 905 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 530 1959 2256 2326 3060 3193 3652 3863 530
Size: 1429 297 70 734 133 459 211 2146
Fragments arranged by size:
2146 1429 734 459 297 211 133 70
TatI w'GTAC_w
Cuts at: 470 774 4994 470
Size: 304 4220 955
Fragments arranged by size:
4220 955 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 4996 5028 646
Size: 32 4318 32 1097
Fragments arranged by size:
4318 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 3274 3274
Size: 5479
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 2972 132
Size: 2840 2639
XhoI C'TCGA_G
Cuts at: 1535 1535
Size: 5479
XmaI C'CCGG_G
Cuts at: 534 641 2497 534
Size: 107 1856 3516
Fragments arranged by size:
3516 1856 107
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BcgI
BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NsiI PacI PshAI PsiI SexAI
SfiI SgrAI SspI SwaI XmnI ZraI