Restriction map for
pF1KSDA0990
(Circular) (Six-base) MAPSORT of: pF1KSDA0990.seq Check: 4021 from: 1 to: 5545
pF1KSDA0990 5545 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 2021 2021
Size: 5545
AccI GT'mk_AC
Cuts at: 2021 2581 2021
Size: 560 4985
Acc65I G'GTAC_C
Cuts at: 2559 2559
Size: 5545
AclI AA'CG_TT
Cuts at: 5385 5385
Size: 5545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1678 2032 2088 3368 3800 4549 4817 247
Size: 1431 354 56 1280 432 749 268 975
Fragments arranged by size:
1431 1280 975 749 432 354 268 56
AflIII A'CryG_T
Cuts at: 644 1535 1760 1795 2212 2895 4001 4978 644
Size: 891 225 35 417 683 1106 977 1211
Fragments arranged by size:
1211 1106 977 891 683 417 225 35
AgeI A'CCGG_T
Cuts at: 3933 3933
Size: 5545
AlwNI CAG_nnn'CTG
Cuts at: 2454 2848 4417 2454
Size: 394 1569 3582
Fragments arranged by size:
3582 1569 394
ApaI G_GGCC'C
Cuts at: 241 241
Size: 5545
ApaLI G'TGCA_C
Cuts at: 4315 4315
Size: 5545
ApoI r'AATT_y
Cuts at: 1013 1169 2041 2547 4898 5154 1013
Size: 156 872 506 2351 256 1404
Fragments arranged by size:
2351 1404 872 506 256 156
AscI GG'CGCG_CC
Cuts at: 144 144
Size: 5545
AseI AT'TA_AT
Cuts at: 20 20
Size: 5545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5545
AvaI C'yCGr_G
Cuts at: 166 212 259 425 2563 166
Size: 46 47 166 2138 3148
Fragments arranged by size:
3148 2138 166 47 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 494 527 494
Size: 33 5512
BamHI G'GATC_C
Cuts at: 1630 1975 2568 1630
Size: 345 593 4607
Fragments arranged by size:
4607 593 345
BanI G'GyrC_C
Cuts at: 245 730 831 2559 2973 3221 3256 245
Size: 485 101 1728 414 248 35 2534
Fragments arranged by size:
2534 1728 485 414 248 101 35
BanII G_rGCy'C
Cuts at: 173 241 704 1106 1959 2557 3587 173
Size: 68 463 402 853 598 1030 2131
Fragments arranged by size:
2131 1030 853 598 463 402 68
BbeI G_GCGC'C
Cuts at: 249 2977 3225 249
Size: 2728 248 2569
Fragments arranged by size:
2728 2569 248
BbsI GAAGACnn'nnnn_
Cuts at: 1798 2329 1798
Size: 531 5014
BciVI GTATCCnnnnn_n'
Cuts at: 3441 4204 5545 3441
Size: 763 1341 3441
Fragments arranged by size:
3441 1341 763
BclI T'GATC_A
Cuts at: 800 1370 3063 800
Size: 570 1693 3282
Fragments arranged by size:
3282 1693 570
BglI GCCn_nnn'nGGC
Cuts at: 263 304 263
Size: 41 5504
BglII A'GATC_T
Cuts at: 996 2229 3058 5097 996
Size: 1233 829 2039 1444
Fragments arranged by size:
2039 1444 1233 829
BlpI GC'TnA_GC
Cuts at: 154 1084 2655 154
Size: 930 1571 3044
Fragments arranged by size:
3044 1571 930
Bme1580I G_kGCm'C
Cuts at: 241 733 3168 3261 4319 241
Size: 492 2435 93 1058 1467
Fragments arranged by size:
2435 1467 1058 492 93
BmgBI CAC'GTC
Cuts at: 645 882 1796 2358 645
Size: 237 914 562 3832
Fragments arranged by size:
3832 914 562 237
BmrI ACTGGGnnnn_n'
Cuts at: 1977 2141 2755 2836 3171 5317 1977
Size: 164 614 81 335 2146 2205
Fragments arranged by size:
2205 2146 614 335 164 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3910 3910
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 781 1171 1961 2208 2299 688
Size: 93 390 790 247 91 3934
Fragments arranged by size:
3934 790 390 247 93 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 568 2719 3598 4092 4390 471
Size: 97 2151 879 494 298 1626
Fragments arranged by size:
2151 1626 879 494 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 653 5249 653
Size: 4596 949
BsaAI yAC'GTr
Cuts at: 2213 2535 2896 2213
Size: 322 361 4862
Fragments arranged by size:
4862 361 322
BsaBI GATnn'nnATC
Cuts at: 6 2384 3057 5096 6
Size: 2378 673 2039 455
Fragments arranged by size:
2378 2039 673 455
BsaHI Gr'CG_yC
Cuts at: 246 2018 2974 3222 3926 5422 246
Size: 1772 956 248 704 1496 369
Fragments arranged by size:
1772 1496 956 704 369 248
BsaWI w'CCGG_w
Cuts at: 902 2732 3253 3933 4207 4354 4765 902
Size: 1830 521 680 274 147 411 1682
Fragments arranged by size:
1830 1682 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2219 2249 2219
Size: 30 5515
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 696 1252 1542 1597 696
Size: 556 290 55 4644
Fragments arranged by size:
4644 556 290 55
BseYI C'CCAG_C
Cuts at: 1191 2270 2968 3726 4305 4801 1191
Size: 1079 698 758 579 496 1935
Fragments arranged by size:
1935 1079 758 698 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1111 940
Size: 171 5374
BsiEI CG_ry'CG
Cuts at: 104 141 3085 3131 4341 104
Size: 37 2944 46 1210 1308
Fragments arranged by size:
2944 1308 1210 46 37
BsiHKAI G_wGCw'C
Cuts at: 1106 1703 2557 3335 3525 4319 1106
Size: 597 854 778 190 794 2332
Fragments arranged by size:
2332 854 794 778 597 190
Bsp1286I G_dGCh'C
Cuts at: 173 241 704 733 1106 1703 1959 2557 3168
Size: 68 463 29 373 597 256 598 611
Cuts at: 3168 3261 3335 3525 3587 4319 173
Size: 93 74 190 62 732 1399
Fragments arranged by size:
1399 732 611 598 597 463 373 256
190 93 74 68 62 29
BspEI T'CCGG_A
Cuts at: 902 4765 902
Size: 3863 1682
BspHI T'CATG_A
Cuts at: 398 575 803 1211 4721 398
Size: 177 228 408 3510 1222
Fragments arranged by size:
3510 1222 408 228 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 1347 1484 2305 2593 2807 3109 3490 425
Size: 922 137 821 288 214 302 381 2480
Fragments arranged by size:
2480 922 821 381 302 288 214 137
BsrBI CCG'CTC
Cuts at: 50 235 3835 3889 5375 5540 50
Size: 185 3600 54 1486 165 55
Fragments arranged by size:
3600 1486 185 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3455 3455
Size: 5545
BsrFI r'CCGG_y
Cuts at: 140 269 298 3541 3722 3933 140
Size: 129 29 3243 181 211 1752
Fragments arranged by size:
3243 1752 211 181 129 29
BsrGI T'GTAC_A
Cuts at: 647 863 1487 647
Size: 216 624 4705
Fragments arranged by size:
4705 624 216
BssHII G'CGCG_C
Cuts at: 133 144 307 319 133
Size: 11 163 12 5359
Fragments arranged by size:
5359 163 12 11
BssSI C'ACGA_G
Cuts at: 1825 3814 4174 1825
Size: 1989 360 3196
Fragments arranged by size:
3196 1989 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1929 2920 1929
Size: 991 4554
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5545
BstYI r'GATC_y
Cuts at: 1 996 1418 1630 1975 2229 2568 3035 3058
Size: 995 422 212 345 254 339 467 23
Cuts at: 3058 3393 3639 4653 4739 4751 5097 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 995 467 449 422 346 345 339
335 254 246 212 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 679 679
Size: 5545
BtgI C'CryG_G
Cuts at: 125 241 431 734 125
Size: 116 190 303 4936
Fragments arranged by size:
4936 303 190 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 329 1096 2067 3493 3674 3837 329
Size: 767 971 1426 181 163 2037
Fragments arranged by size:
2037 1426 971 767 181 163
BtsI GCAGTG_nn'
Cuts at: 928 2786 928
Size: 1858 3687
DraI TTT'AAA
Cuts at: 2542 2542
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 1348 2439 3249 4109 1348
Size: 1091 810 860 2784
Fragments arranged by size:
2784 1091 860 810
EaeI y'GGCC_r
Cuts at: 128 138 337 434 1293 1604 3128 3302 3693
Size: 10 199 97 859 311 1524 174 391
Cuts at: 3693 3720 5067 128
Size: 27 1347 606
Fragments arranged by size:
1524 1347 859 606 391 311 199 174
97 27 10
EagI C'GGCC_G
Cuts at: 138 3128 138
Size: 2990 2555
EarI CTCTTCn'nnn_
Cuts at: 391 1302 1687 2011 3566 3776 391
Size: 911 385 324 1555 210 2160
Fragments arranged by size:
2160 1555 911 385 324 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1096 1311 3751 4063 4209 5355 1096
Size: 215 2440 312 146 1146 1286
Fragments arranged by size:
2440 1286 1146 312 215 146
EcoICRI GAG'CTC
Cuts at: 1104 2555 1104
Size: 1451 4094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 688 781 1171 1678 1961 2032 2088 2208
Size: 441 93 390 507 283 71 56 120
Cuts at: 2208 2299 3368 3800 4549 4817 247
Size: 91 1069 432 749 268 975
Fragments arranged by size:
1069 975 749 507 441 432 390 283
268 120 93 91 71 56
EcoNI CCTnn'n_nnAGG
Cuts at: 1212 1307 1212
Size: 95 5450
EcoO109I rG'GnC_Cy
Cuts at: 790 1182 2682 790
Size: 392 1500 3653
Fragments arranged by size:
3653 1500 392
EcoRI G'AATT_C
Cuts at: 2041 2547 2041
Size: 506 5039
EcoRV GAT'ATC
Cuts at: 1097 1097
Size: 5545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 588 3562 588
Size: 2974 2571
FspI TGC'GCA
Cuts at: 3324 3324
Size: 5545
HaeII r_GCGC'y
Cuts at: 249 2977 3225 4249 5228 249
Size: 2728 248 1024 979 566
Fragments arranged by size:
2728 1024 979 566 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1385 1417 1602 1634 2219 2251 3385 3415 3417
Size: 32 185 32 585 32 1134 30 2
Cuts at: 3417 3447 1385
Size: 30 3483
Fragments arranged by size:
3483 1134 585 185 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1290 2022 2582 1290
Size: 732 560 4253
Fragments arranged by size:
4253 732 560
KasI G'GCGC_C
Cuts at: 245 2973 3221 245
Size: 2728 248 2569
Fragments arranged by size:
2728 2569 248
KpnI G_GTAC'C
Cuts at: 2563 2563
Size: 5545
MluI A'CGCG_T
Cuts at: 1535 4978 1535
Size: 3443 2102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1877 4216 4400 1877
Size: 2339 184 3022
Fragments arranged by size:
3022 2339 184
MscI TGG'CCA
Cuts at: 1606 3304 1606
Size: 1698 3847
MslI CAynn'nnrTG
Cuts at: 1351 1366 1765 3532 3659 1351
Size: 15 399 1767 127 3237
Fragments arranged by size:
3237 1767 399 127 15
MspA1I CmG'CkG
Cuts at: 1125 1191 2454 2654 2968 3328 4343 4588 1125
Size: 66 1263 200 314 360 1015 245 2082
Fragments arranged by size:
2082 1263 1015 360 314 245 200 66
NaeI GCC'GGC
Cuts at: 142 300 3724 142
Size: 158 3424 1963
Fragments arranged by size:
3424 1963 158
NarI GG'CG_CC
Cuts at: 246 2974 3222 246
Size: 2728 248 2569
Fragments arranged by size:
2728 2569 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5545
NgoMIV G'CCGG_C
Cuts at: 140 298 3722 140
Size: 158 3424 1963
Fragments arranged by size:
3424 1963 158
NruI TCG'CGA
Cuts at: 3906 3906
Size: 5545
NspI r_CATG'y
Cuts at: 594 1083 1764 1923 2596 3096 4005 594
Size: 489 681 159 673 500 909 2134
Fragments arranged by size:
2134 909 681 673 500 489 159
PasI CC'CwG_GG
Cuts at: 1684 1684
Size: 5545
PciI A'CATG_T
Cuts at: 1760 4001 1760
Size: 2241 3304
PflMI CCAn_nnn'nTGG
Cuts at: 606 1572 606
Size: 966 4579
PfoI T'CCnGG_A
Cuts at: 854 1469 854
Size: 615 4930
PmeI GTTT'AAAC
Cuts at: 2542 2542
Size: 5545
PmlI CAC'GTG
Cuts at: 2213 2213
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4684 4716 4684
Size: 32 5513
PpuMI rG'GwC_Cy
Cuts at: 1182 1182
Size: 5545
PsiI TTA'TAA
Cuts at: 2222 2222
Size: 5545
PspOMI G'GGCC_C
Cuts at: 237 237
Size: 5545
PstI C_TGCA'G
Cuts at: 422 2590 3275 422
Size: 2168 685 2692
Fragments arranged by size:
2692 2168 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5545
PvuII CAG'CTG
Cuts at: 1125 1191 2454 2968 3328 1125
Size: 66 1263 514 360 3342
Fragments arranged by size:
3342 1263 514 360 66
RsrII CG'GwC_CG
Cuts at: 272 3738 272
Size: 3466 2079
SacI G_AGCT'C
Cuts at: 1106 2557 1106
Size: 1451 4094
SalI G'TCGA_C
Cuts at: 2020 2580 2020
Size: 560 4985
SapI GCTCTTCn'nnn_
Cuts at: 2011 3566 3776 2011
Size: 1555 210 3780
Fragments arranged by size:
3780 1555 210
SbfI CC_TGCA'GG
Cuts at: 2590 2590
Size: 5545
ScaI AGT'ACT
Cuts at: 5062 5062
Size: 5545
SfcI C'TryA_G
Cuts at: 33 418 441 2036 2586 3271 4266 4457 33
Size: 385 23 1595 550 685 995 191 1121
Fragments arranged by size:
1595 1121 995 685 550 385 191 23
SfoI GGC'GCC
Cuts at: 247 2975 3223 247
Size: 2728 248 2569
Fragments arranged by size:
2728 2569 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5545
SmaI CCC'GGG
Cuts at: 214 2565 214
Size: 2351 3194
SmlI C'TyrA_G
Cuts at: 486 583 2698 3613 4107 4369 486
Size: 97 2115 915 494 262 1662
Fragments arranged by size:
2115 1662 915 494 262 97
SnaBI TAC'GTA
Cuts at: 2535 2535
Size: 5545
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5545
SphI G_CATG'C
Cuts at: 2596 3096 2596
Size: 500 5045
StuI AGG'CCT
Cuts at: 725 725
Size: 5545
StyI C'CwwG_G
Cuts at: 125 1337 2265 2677 3031 125
Size: 1212 928 412 354 2639
Fragments arranged by size:
2639 1212 928 412 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 886 2233 3126 3259 3718 3929 886
Size: 1347 893 133 459 211 2502
Fragments arranged by size:
2502 1347 893 459 211 133
TatI w'GTAC_w
Cuts at: 587 647 863 1061 1463 1487 2402 5060 587
Size: 60 216 198 402 24 915 2658 1072
Fragments arranged by size:
2658 1072 915 402 216 198 60 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5062 5094 5062
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1348 2026 3340 1348
Size: 678 1314 3553
Fragments arranged by size:
3553 1314 678
XbaI T'CTAG_A
Cuts at: 2574 2574
Size: 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 2451 3038 741
Size: 1710 587 3248
Fragments arranged by size:
3248 1710 587
XmaI C'CCGG_G
Cuts at: 212 2563 212
Size: 2351 3194
XmnI GAAnn'nnTTC
Cuts at: 571 2045 571
Size: 1474 4071
ZraI GAC'GTC
Cuts at: 2019 2019
Size: 5545
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AvrII
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI
BstEII BstXI BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI